BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon23_contig00035014
         (847 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020245410.1| cytochrome P450 CYP736A12-like, partial [Asp...   327   e-106
ref|XP_015867271.1| PREDICTED: cytochrome P450 CYP736A12-like [Z...   254   4e-78
ref|XP_002310011.2| hypothetical protein POPTR_0007s06310g [Popu...   252   2e-77
ref|XP_002310007.2| cytochrome P450 family protein [Populus tric...   252   3e-77
gb|PNT27812.1| hypothetical protein POPTR_007G085000v3 [Populus ...   248   1e-75
ref|XP_002310008.2| hypothetical protein POPTR_0007s06270g [Popu...   248   1e-75
ref|XP_002310005.2| hypothetical protein POPTR_0007s06240g [Popu...   248   1e-75
ref|XP_021298187.1| cytochrome P450 CYP736A12-like [Herrania umb...   248   2e-75
gb|EXB56947.1| Cytochrome P450 71A1 [Morus notabilis]                 247   3e-75
ref|XP_010094781.1| cytochrome P450 CYP736A12 isoform X1 [Morus ...   247   3e-75
ref|XP_010094782.2| cytochrome P450 CYP736A12 [Morus notabilis]       247   3e-75
ref|XP_011025648.1| PREDICTED: cytochrome P450 CYP736A12-like [P...   241   3e-75
gb|PNT27798.1| hypothetical protein POPTR_007G083600v3 [Populus ...   247   3e-75
gb|PNT27790.1| hypothetical protein POPTR_007G082900v3 [Populus ...   247   3e-75
ref|XP_002310015.1| cytochrome P450 family protein [Populus tric...   247   3e-75
ref|XP_002310016.2| hypothetical protein POPTR_0007s06370g [Popu...   247   3e-75
ref|XP_011013422.1| PREDICTED: cytochrome P450 CYP736A12-like [P...   246   7e-75
ref|XP_002310014.1| cytochrome P450 family protein [Populus tric...   245   1e-74
ref|XP_022978478.1| cytochrome P450 CYP736A12-like [Cucurbita ma...   244   3e-74
gb|ACM89788.1| cytochrome P450 monooxygenase CYP736B [Vitis ariz...   244   3e-74

>ref|XP_020245410.1| cytochrome P450 CYP736A12-like, partial [Asparagus officinalis]
          Length = 467

 Score =  327 bits (837), Expect = e-106
 Identities = 161/255 (63%), Positives = 204/255 (80%), Gaps = 4/255 (1%)
 Frame = -3

Query: 755 RPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPTVFISSPSLAEQVLKSHDIVF 576
           RP PIIG+LH LSP PHRDV+ L+KTYGPI++LRLGS PTVFISSPSL E++ K+HD+  
Sbjct: 1   RPLPIIGNLHSLSPFPHRDVSNLTKTYGPIIHLRLGSTPTVFISSPSLVERLFKNHDVAI 60

Query: 575 ANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFLNPAKLESYEFIRKEEMADFI 396
           ++R  AEA E LSYG K   F+EYG +WR  R++CV+  L+ + LES+E +R+EEM  F+
Sbjct: 61  SDRSPAEAQEILSYGRKGFGFSEYGSYWRKVRRICVNSLLSKSGLESFEPVRREEMRIFV 120

Query: 395 ESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEENMDGAKFSEHIHKVMRLLGSF 216
           +S+K+AA  RA VDVTAKVRLLVAD+VCR+VMGRKCMEENMDG K SEH  +VMR+LGSF
Sbjct: 121 DSLKEAAADRAVVDVTAKVRLLVADIVCRVVMGRKCMEENMDGGKLSEHFDEVMRILGSF 180

Query: 215 NLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHERRREEGKNQ---NDFVGLML 45
           NLND LP L+ FDLQG+R++T  V+E IDRFLEKI+ EH + REEG  +   NDFV ++L
Sbjct: 181 NLNDTLPFLRPFDLQGIRRRTMTVREKIDRFLEKIIHEHLQSREEGMKEHKKNDFVDVLL 240

Query: 44  DCINDKD-LEVELDR 3
           D ++DK+ LE+ELDR
Sbjct: 241 DAMDDKENLELELDR 255


>ref|XP_015867271.1| PREDICTED: cytochrome P450 CYP736A12-like [Ziziphus jujuba]
          Length = 495

 Score =  254 bits (649), Expect = 4e-78
 Identities = 126/281 (44%), Positives = 189/281 (67%)
 Frame = -3

Query: 845 SVVLPLALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPI 666
           +++  L L ++L + +  +N ++ RLPPGPR FPI GSLHLL   PHRD+ +L+K YGPI
Sbjct: 6   TILALLLLAYTLLWKI--TNNNNKRLPPGPRGFPIFGSLHLLGEFPHRDLHRLAKQYGPI 63

Query: 665 LYLRLGSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRG 486
           +++RLG +PT+ +SSP  AE  LK+HD+VFA+RP  EAA+ +SY  K++AF EYG +WR 
Sbjct: 64  MHIRLGLIPTIVVSSPKAAELFLKTHDLVFASRPPHEAAKHISYNQKSMAFAEYGSYWRD 123

Query: 485 ARKLCVSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRI 306
            RK+C    L+  K+ S+  +RKEE+  FI+S++K+A  R  VD++AKV  L ADM CR+
Sbjct: 124 VRKMCTLELLSNVKINSFRAMRKEEVGLFIDSLRKSARDRVAVDLSAKVSSLSADMSCRM 183

Query: 305 VMGRKCMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDR 126
           V G+K  +   D   F   I + M+L  + NL DY+P +   DLQG+ K+ ++V +  D 
Sbjct: 184 VFGKKFADSEFDERGFKAVIQEGMQLSAAPNLGDYIPPIASLDLQGLTKKMKKVGKVFDD 243

Query: 125 FLEKIVDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           F EKI+DEH + ++E KN+ DFV +ML  +  ++ E  ++R
Sbjct: 244 FFEKIIDEHAQSKDEDKNK-DFVDVMLGFLGSEESEYRIER 283


>ref|XP_002310011.2| hypothetical protein POPTR_0007s06310g [Populus trichocarpa]
 gb|PNT27804.1| hypothetical protein POPTR_007G084200v3 [Populus trichocarpa]
          Length = 493

 Score =  252 bits (644), Expect = 2e-77
 Identities = 123/271 (45%), Positives = 184/271 (67%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           +L F LQ  +  + RLPPGPR FPI GSLHLL   PHR   +L++ YGPI++LRLG VPT
Sbjct: 12  ALIFLLQWLSTKNKRLPPGPRGFPIFGSLHLLGKFPHRAFHQLAQKYGPIMHLRLGLVPT 71

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AE  LK+HD+VFA RP  E+A ++SYG K+V F++YG +WR  RK+C    L
Sbjct: 72  IVVSSPEAAELFLKTHDLVFAGRPPHESARYISYGQKSVTFSQYGSYWRNMRKMCTVELL 131

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++  R EE+   I+ +++AA+ R  VD++AKV  L ADM CR+V G+K ++E+
Sbjct: 132 SSLKITSFKSTRMEELDLLIKYIQEAAQERVAVDLSAKVSSLSADMSCRMVFGKKYLDED 191

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   + +VM L  + NL DY+P +   DLQG+ K+ + + + +D F+ KI+DEH 
Sbjct: 192 LDERGFKSVMQEVMHLSAAPNLGDYIPQIGALDLQGLTKRMKAISKVLDLFVSKIIDEHA 251

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           + +E+GKN+ DFV +ML C+  ++ E  +D+
Sbjct: 252 QYQEKGKNK-DFVDVMLSCMKSEENEYLVDQ 281


>ref|XP_002310007.2| cytochrome P450 family protein [Populus trichocarpa]
 gb|PNT27810.1| hypothetical protein POPTR_007G084800v3 [Populus trichocarpa]
          Length = 497

 Score =  252 bits (643), Expect = 3e-77
 Identities = 128/276 (46%), Positives = 179/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL   PH+D+ +L+  YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHQDLHQLANKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK+HD+VFANRP  EAA+ +SY  K+++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTHDLVFANRPPNEAAKHISYEQKSLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD++AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSSRKEELDLLIDYIKDASRERVAVDLSAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            ME+  D   F   IH+ MRL  SFN  DY+P +   DLQG+ K+ + V +  D FLEKI
Sbjct: 191 YMEKEFDDKGFKPVIHEGMRLAASFNFGDYIPPIAPLDLQGLTKRMKAVGKVFDDFLEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDFLGSEETEYSIGR 285


>gb|PNT27812.1| hypothetical protein POPTR_007G085000v3 [Populus trichocarpa]
          Length = 493

 Score =  248 bits (633), Expect = 1e-75
 Identities = 123/271 (45%), Positives = 183/271 (67%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           +L F LQ  +  + RLPPGPR FPI GSLHLL   PHR + +L++ YGPI++LRLG VPT
Sbjct: 12  ALIFLLQWLSTKNKRLPPGPRGFPIFGSLHLLGKFPHRALHQLAQKYGPIMHLRLGLVPT 71

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AE  LK+HD+VFA RP  EAA ++SYG K +AF +YG +WR  RK+C    L
Sbjct: 72  IVVSSPEAAELFLKTHDLVFAGRPPHEAARYISYGQKGMAFAQYGSYWRNMRKMCTVELL 131

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++ +R EE+   I+ +++AA+ R  VD++AKV  L ADM CR+V G+K ++E+
Sbjct: 132 SSLKITSFKPMRMEELDLLIKYIQEAAQERVAVDMSAKVSSLSADMSCRMVFGKKYVDED 191

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   + +VM L  + +L DY+P +   DLQG+ K+   + +  D FL+KI+DEH 
Sbjct: 192 LDERGFKSVMQEVMHLTAAPHLGDYIPQIAALDLQGLTKRMNAISKVFDVFLDKIIDEHV 251

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           + +E+GKN+ DFV +ML  +  ++ E  +D+
Sbjct: 252 QYQEKGKNK-DFVDVMLSLMKSEENEYLVDQ 281


>ref|XP_002310008.2| hypothetical protein POPTR_0007s06270g [Populus trichocarpa]
 gb|PNT27809.1| hypothetical protein POPTR_007G084700v3 [Populus trichocarpa]
          Length = 493

 Score =  248 bits (633), Expect = 1e-75
 Identities = 122/271 (45%), Positives = 185/271 (68%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           +L F LQ  +  + RLPPGPR FPI GSLHLL   PHR + +L++ YGPI++LRLG VPT
Sbjct: 12  ALIFLLQWLSTKNKRLPPGPRGFPIFGSLHLLGKFPHRALHQLAQKYGPIMHLRLGLVPT 71

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AE  LK+HD+VFA RP  E+A ++SYG K +AF +YG +WR  RK+C    L
Sbjct: 72  IVVSSPEAAELFLKTHDLVFAGRPPHESARYISYGQKGMAFAQYGSYWRNIRKMCTVELL 131

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++ +R EE+   I+ +++AA+ R  VD++AKV  L ADM CR+V G+K ++E+
Sbjct: 132 SSLKITSFKPMRMEELDLLIKYIQEAAQERVAVDLSAKVSSLSADMSCRMVFGKKYLDED 191

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   + +VM L  + +L DY+P +   DLQG+ K+ + + + +D FL+KI+DEH 
Sbjct: 192 LDERGFKSVMQEVMHLSAAPHLGDYIPQIAALDLQGLTKRMKAISKVLDVFLDKIIDEHV 251

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           + +E+GKN+ DFV +ML  +  ++ E  +D+
Sbjct: 252 QYQEKGKNK-DFVDVMLSFMKSEENEYLVDQ 281


>ref|XP_002310005.2| hypothetical protein POPTR_0007s06240g [Populus trichocarpa]
          Length = 500

 Score =  248 bits (633), Expect = 1e-75
 Identities = 123/271 (45%), Positives = 183/271 (67%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           +L F LQ  +  + RLPPGPR FPI GSLHLL   PHR + +L++ YGPI++LRLG VPT
Sbjct: 12  ALIFLLQWLSTKNKRLPPGPRGFPIFGSLHLLGKFPHRALHQLAQKYGPIMHLRLGLVPT 71

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AE  LK+HD+VFA RP  EAA ++SYG K +AF +YG +WR  RK+C    L
Sbjct: 72  IVVSSPEAAELFLKTHDLVFAGRPPHEAARYISYGQKGMAFAQYGSYWRNMRKMCTVELL 131

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++ +R EE+   I+ +++AA+ R  VD++AKV  L ADM CR+V G+K ++E+
Sbjct: 132 SSLKITSFKPMRMEELDLLIKYIQEAAQERVAVDMSAKVSSLSADMSCRMVFGKKYVDED 191

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   + +VM L  + +L DY+P +   DLQG+ K+   + +  D FL+KI+DEH 
Sbjct: 192 LDERGFKSVMQEVMHLTAAPHLGDYIPQIAALDLQGLTKRMNAISKVFDVFLDKIIDEHV 251

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           + +E+GKN+ DFV +ML  +  ++ E  +D+
Sbjct: 252 QYQEKGKNK-DFVDVMLSLMKSEENEYLVDQ 281


>ref|XP_021298187.1| cytochrome P450 CYP736A12-like [Herrania umbratica]
          Length = 514

 Score =  248 bits (633), Expect = 2e-75
 Identities = 122/274 (44%), Positives = 181/274 (66%), Gaps = 4/274 (1%)
 Frame = -3

Query: 842 VVLPLALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPIL 663
           ++L   LC  ++    K  ++  +LPPGP P P+IG+LHLL  LPH+ +  L+K YGPI+
Sbjct: 10  LILLGTLCSFIYISRPKQLKNGRKLPPGPHPMPVIGNLHLLGKLPHQSLYHLAKRYGPIM 69

Query: 662 YLRLGSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGA 483
            LRLG VPT+ +SSP  AE  LK+HD+VFA+RP  +AAE+LSYG K + FTEYG +WR  
Sbjct: 70  SLRLGYVPTIVVSSPQAAELFLKTHDVVFASRPKVQAAEYLSYGTKGMGFTEYGSYWRTV 129

Query: 482 RKLCVSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIV 303
           RK C  H L+P+K+E +  +RK E+   +E+VKKAA A  TVD++ KV  L+ D++ +++
Sbjct: 130 RKWCTLHLLSPSKVEYFAPVRKAELGSMVETVKKAAAAGETVDISGKVGELIEDIMHKMI 189

Query: 302 MGRKCMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRF 123
            GR    E+ +       I ++ RL G+FN++DY+P L   DLQG+ ++ + V + +D+F
Sbjct: 190 FGRPSNSED-ENLNLKLLIKEISRLAGAFNISDYIPYLAPLDLQGLSRKLKRVSKILDKF 248

Query: 122 LEKIVDEHER----RREEGKNQNDFVGLMLDCIN 33
           LEKI+DEHE+      EE K   +FV +M+  +N
Sbjct: 249 LEKIIDEHEQAVGSNPEEQKPHRNFVHVMVSLLN 282


>gb|EXB56947.1| Cytochrome P450 71A1 [Morus notabilis]
          Length = 507

 Score =  247 bits (631), Expect = 3e-75
 Identities = 125/276 (45%), Positives = 179/276 (64%), Gaps = 5/276 (1%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           L++ FS      + N    +LPPGP P P+IG+LH L+ LPHR +  LSK YGPI+ LRL
Sbjct: 13  LSIIFSRAAAKIRRNNGRRKLPPGPTPLPVIGNLHQLTTLPHRGLQSLSKKYGPIMSLRL 72

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VP V +SSP  AE  LK+ D  FA+RP  +++E++SYG K +AFTEYGP+WR  RKLC
Sbjct: 73  GQVPAVVVSSPEAAELFLKTFDTTFASRPRVQSSEYMSYGTKGMAFTEYGPYWRNVRKLC 132

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ES+  +R+E++A  +ES++ +A AR  VD++ K+  L+ADM CR+++G  
Sbjct: 133 TLQLLSALKIESFAALRREDVASVVESLRASAAAREVVDISRKIGELIADMSCRMILGIS 192

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
             + N       E +H+ M L G+FNL+DY+P L   DLQG  ++ ++  E IDR LE+I
Sbjct: 193 LEDRN----HLKELVHEAMFLSGAFNLSDYVPYLAPLDLQGYTRRMKKNSEAIDRVLEEI 248

Query: 110 VDEHER-RREEGKNQNDFVGLMLDCIND----KDLE 18
           + EHER   E+G  Q DFV ++L  +N     +DLE
Sbjct: 249 ITEHERGTSEKGHRQKDFVDVLLSLVNQPMNPQDLE 284


>ref|XP_010094781.1| cytochrome P450 CYP736A12 isoform X1 [Morus notabilis]
 ref|XP_024020269.1| cytochrome P450 CYP736A12 isoform X2 [Morus notabilis]
 gb|EXB56946.1| Cytochrome P450 71D8 [Morus notabilis]
          Length = 508

 Score =  247 bits (631), Expect = 3e-75
 Identities = 124/271 (45%), Positives = 181/271 (66%), Gaps = 2/271 (0%)
 Frame = -3

Query: 839 VLPLALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILY 660
           +L + L   + F +Q  NR+  +LPPGPRP PIIG+LH+L+PLPHR +  L+K YGPI+ 
Sbjct: 8   ILLVILGSIISFLVQIHNRNGRKLPPGPRPLPIIGNLHMLTPLPHRGLQALAKKYGPIMS 67

Query: 659 LRLGSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGAR 480
           +RLG VPTV +SSP  AE +L++HD +FA+RP  +A+ +LSYG K +AF EYGP+WR  R
Sbjct: 68  MRLGRVPTVVVSSPQAAELILQTHDAIFASRPKVQASAYLSYGSKGMAFNEYGPYWRNVR 127

Query: 479 KLCVSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVM 300
           K C    L  +K  S+  +R  E+A  + S++ AA A   VDV++KV  LVAD+ CRIV+
Sbjct: 128 KFCTLQLLTSSKTASFAALRAAEVASLVASLRAAAAAGEVVDVSSKVGRLVADISCRIVL 187

Query: 299 GRKCMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFL 120
           GR   +E  D       + ++MRL G+FNL DY+P L   DLQG  +Q ++V + +D+ L
Sbjct: 188 GRNVGDERYD---LKRLVEEIMRLTGAFNLADYVPYLAPLDLQGYTRQLKKVNKEMDKVL 244

Query: 119 EKIVDEHER--RREEGKNQNDFVGLMLDCIN 33
           E+I+ EHE   R ++G + +DF+ ++L  +N
Sbjct: 245 EEIIAEHEEVGRYKQG-HHDDFIDVLLSLMN 274


>ref|XP_010094782.2| cytochrome P450 CYP736A12 [Morus notabilis]
          Length = 509

 Score =  247 bits (631), Expect = 3e-75
 Identities = 125/276 (45%), Positives = 179/276 (64%), Gaps = 5/276 (1%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           L++ FS      + N    +LPPGP P P+IG+LH L+ LPHR +  LSK YGPI+ LRL
Sbjct: 15  LSIIFSRAAAKIRRNNGRRKLPPGPTPLPVIGNLHQLTTLPHRGLQSLSKKYGPIMSLRL 74

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VP V +SSP  AE  LK+ D  FA+RP  +++E++SYG K +AFTEYGP+WR  RKLC
Sbjct: 75  GQVPAVVVSSPEAAELFLKTFDTTFASRPRVQSSEYMSYGTKGMAFTEYGPYWRNVRKLC 134

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ES+  +R+E++A  +ES++ +A AR  VD++ K+  L+ADM CR+++G  
Sbjct: 135 TLQLLSALKIESFAALRREDVASVVESLRASAAAREVVDISRKIGELIADMSCRMILGIS 194

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
             + N       E +H+ M L G+FNL+DY+P L   DLQG  ++ ++  E IDR LE+I
Sbjct: 195 LEDRN----HLKELVHEAMFLSGAFNLSDYVPYLAPLDLQGYTRRMKKNSEAIDRVLEEI 250

Query: 110 VDEHER-RREEGKNQNDFVGLMLDCIND----KDLE 18
           + EHER   E+G  Q DFV ++L  +N     +DLE
Sbjct: 251 ITEHERGTSEKGHRQKDFVDVLLSLVNQPMNPQDLE 286


>ref|XP_011025648.1| PREDICTED: cytochrome P450 CYP736A12-like [Populus euphratica]
          Length = 300

 Score =  241 bits (614), Expect = 3e-75
 Identities = 119/276 (43%), Positives = 174/276 (63%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           + L F L  +L K      +LPPGP  FPI GSLHLL   PH D+ +L+K YGPI+Y+RL
Sbjct: 11  IPLAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHHDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
             VPTV +SSP  AE +LK+HD+VFA+RP  EAA+ ++Y  K + F  YG +WR  RK+C
Sbjct: 71  SLVPTVVVSSPRAAELILKTHDLVFASRPPNEAAKHITYEQKGLTFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD+ +K+  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDIGSKISSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H++MRL  SFN+ DY+P +   DLQG+ K+ + + +  D F EKI
Sbjct: 191 YLEKEFDEMGFKPVTHELMRLAASFNMGDYIPPIAPLDLQGLTKRMKAIGKLFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDVLGSEETEYRIGR 285


>gb|PNT27798.1| hypothetical protein POPTR_007G083600v3 [Populus trichocarpa]
          Length = 497

 Score =  247 bits (630), Expect = 3e-75
 Identities = 126/276 (45%), Positives = 178/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL  LPH D+ +L+K YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKLPHHDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK++D+VFA+RP  EAA+ +SY  K ++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD+ AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H++MRL  SFNL DY+P +   DLQG+ K+ + V +  D F EKI
Sbjct: 191 YLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDFLGSEETEYRIGR 285


>gb|PNT27790.1| hypothetical protein POPTR_007G082900v3 [Populus trichocarpa]
          Length = 497

 Score =  247 bits (630), Expect = 3e-75
 Identities = 126/276 (45%), Positives = 178/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL  LPH D+ +L+K YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKLPHHDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK++D+VFA+RP  EAA+ +SY  K ++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD+ AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H++MRL  SFNL DY+P +   DLQG+ K+ + V +  D F EKI
Sbjct: 191 YLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDFLGSEETEYRIGR 285


>ref|XP_002310015.1| cytochrome P450 family protein [Populus trichocarpa]
          Length = 497

 Score =  247 bits (630), Expect = 3e-75
 Identities = 125/276 (45%), Positives = 178/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL   PH+D+ +L+K YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHQDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK++D+VFANRP  EAA+ ++Y  K ++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTNDLVFANRPPNEAAKHITYEQKNLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD++AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLSAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H+ MRL  SFNL DY+P +   DLQG+ K+ + V +  D F EKI
Sbjct: 191 YLEKEFDEKGFKPLTHEAMRLTASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQIKDENRTK-DFVDVMLDFLGSEETEYRIGR 285


>ref|XP_002310016.2| hypothetical protein POPTR_0007s06370g [Populus trichocarpa]
 gb|PNT27793.1| hypothetical protein POPTR_007G083200v3 [Populus trichocarpa]
 gb|PNT27797.1| hypothetical protein POPTR_007G083500v3 [Populus trichocarpa]
          Length = 497

 Score =  247 bits (630), Expect = 3e-75
 Identities = 126/276 (45%), Positives = 178/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL  LPH D+ +L+K YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKLPHHDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK++D+VFA+RP  EAA+ +SY  K ++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD+ AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H++MRL  SFNL DY+P +   DLQG+ K+ + V +  D F EKI
Sbjct: 191 YLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDFLGSEETEYRIGR 285


>ref|XP_011013422.1| PREDICTED: cytochrome P450 CYP736A12-like [Populus euphratica]
          Length = 493

 Score =  246 bits (627), Expect = 7e-75
 Identities = 121/271 (44%), Positives = 181/271 (66%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           +L F LQ  +  + RLPPGPR FPI GSLH+L   PHR + +L++ YGPI++LRLG VPT
Sbjct: 12  ALIFLLQWLSTKNKRLPPGPRGFPIFGSLHMLGKFPHRALHQLAQKYGPIMHLRLGLVPT 71

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AE  LK+HD+VFA RP  EAA + SYG K + F +YGP+WR  RK+C    L
Sbjct: 72  IVVSSPEAAELFLKTHDLVFAGRPPHEAARYFSYGQKGMTFAQYGPYWRNMRKMCTIELL 131

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++  R EE+   I+ +++AA  R  VD++AKV  L ADM CR+V G+K ++E+
Sbjct: 132 SSLKITSFKSTRMEELDLLIKYIQEAARERVAVDLSAKVSSLNADMSCRMVFGKKYLDED 191

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   + +VM L  + +L DY+P +   DLQG+ K+ + + + +D +L KI+DEH 
Sbjct: 192 LDERGFKSVMQEVMHLSAAPHLGDYIPQIAALDLQGLTKRMKAISKVLDVYLNKIIDEHA 251

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           + +E+GKN+ DFV +ML  +  ++ E  +D+
Sbjct: 252 QYQEKGKNK-DFVDVMLSFMKSEENEHLVDQ 281


>ref|XP_002310014.1| cytochrome P450 family protein [Populus trichocarpa]
          Length = 497

 Score =  245 bits (626), Expect = 1e-74
 Identities = 125/276 (45%), Positives = 177/276 (64%)
 Frame = -3

Query: 830 LALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRL 651
           +AL F L  +L K      +LPPGP  FPI GSLHLL   PH D+ +L+K YGPI+Y+RL
Sbjct: 11  IALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHHDLHQLAKKYGPIMYMRL 70

Query: 650 GSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLC 471
           G VPTV +SSP  AE +LK++D+VFA+RP  EAA+ +SY  K ++F  YG +WR  RK+C
Sbjct: 71  GLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPYGSYWRNVRKMC 130

Query: 470 VSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRK 291
               L+  K+ S+   RKEE+   I+ +K A+  R  VD+ AKV  L AD+ CR+V G+K
Sbjct: 131 TLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSADISCRMVFGKK 190

Query: 290 CMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKI 111
            +E+  D   F    H++MRL  SFNL DY+P +   DLQG+ K+ + V +  D F EKI
Sbjct: 191 YLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAVGKVFDDFFEKI 250

Query: 110 VDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           +DEH + ++E + + DFV +MLD +  ++ E  + R
Sbjct: 251 IDEHIQFKDENRTK-DFVDVMLDFLGSEETEYSIGR 285


>ref|XP_022978478.1| cytochrome P450 CYP736A12-like [Cucurbita maxima]
          Length = 494

 Score =  244 bits (623), Expect = 3e-74
 Identities = 116/280 (41%), Positives = 184/280 (65%)
 Frame = -3

Query: 842 VVLPLALCFSLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPIL 663
           ++L +AL     F+    +R   +LPPGPR  PI+GSLHLL  LPHRD+  LS+ YG I+
Sbjct: 4   ILLAIALLGIALFFTSWLHRGPNQLPPGPRGLPILGSLHLLGKLPHRDLRALSQKYGSIM 63

Query: 662 YLRLGSVPTVFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGA 483
           Y+RLG VPT+ +SSP  AE  LK+HDIVFA+RP+ +A++++SYG K +AF  YG +WR  
Sbjct: 64  YMRLGLVPTIVVSSPQAAELFLKTHDIVFASRPFVQASKYMSYGQKNLAFAPYGSYWRNM 123

Query: 482 RKLCVSHFLNPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIV 303
           RK+C    L+  K+ S+  +R+EE+   ++ ++ AA+ RA V++++KV  ++ DM C +V
Sbjct: 124 RKMCTLELLSNVKVNSFRSMRREELGLLVDYLRDAAKNRAVVNLSSKVCGVITDMTCLMV 183

Query: 302 MGRKCMEENMDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRF 123
            G+K  ++  D   F   I + M L  + N+ D++P +   DLQG+ ++++ + +  D F
Sbjct: 184 FGKKYADQEFDERGFKSVIQEAMHLAATPNMGDFIPQIAALDLQGLDRRSKAISKIFDGF 243

Query: 122 LEKIVDEHERRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
           LE+IVD+H   + E K + DFV +MLD +N K+ E +++R
Sbjct: 244 LERIVDDHLESKHENKTK-DFVDVMLDIMNSKETEYQIER 282


>gb|ACM89788.1| cytochrome P450 monooxygenase CYP736B [Vitis arizonica x Vitis
           rupestris]
 gb|ACN89833.1| cytochrome P450 monooxygenase CYP736B [Vitis arizonica x Vitis
           rupestris]
          Length = 495

 Score =  244 bits (623), Expect = 3e-74
 Identities = 119/271 (43%), Positives = 175/271 (64%)
 Frame = -3

Query: 815 SLFFYLQKSNRSSYRLPPGPRPFPIIGSLHLLSPLPHRDVAKLSKTYGPILYLRLGSVPT 636
           ++ F +        RLPPGPR  PI+G++H+L  LPHR +  LSK YGPI+Y+RLG VP 
Sbjct: 13  AIIFLINIVKNKHKRLPPGPRGIPILGNMHMLGSLPHRALQALSKKYGPIMYMRLGFVPA 72

Query: 635 VFISSPSLAEQVLKSHDIVFANRPYAEAAEFLSYGFKAVAFTEYGPHWRGARKLCVSHFL 456
           + +SSP  AEQ LK+HD+VFANRP  E +  + Y  K ++F+ YGP+WR  RKLC+   L
Sbjct: 73  IVVSSPQAAEQFLKTHDLVFANRPPHECSRHMLYDGKGISFSGYGPYWRSMRKLCILELL 132

Query: 455 NPAKLESYEFIRKEEMADFIESVKKAAEARATVDVTAKVRLLVADMVCRIVMGRKCMEEN 276
           +  K+ S++ +R+EE+   I+S ++AA A A VDV+AKV LL ADM CR+V G+K M+++
Sbjct: 133 SSHKINSFKPMRREEVGLLIKSFEEAARAGAAVDVSAKVALLSADMSCRMVFGKKYMDKD 192

Query: 275 MDGAKFSEHIHKVMRLLGSFNLNDYLPVLKLFDLQGVRKQTREVKEPIDRFLEKIVDEHE 96
           +D   F   I +VM+L  + N+ DY+P L   DLQG+ ++ +   +  D F EKI+DEH 
Sbjct: 193 LDERGFKAVIKEVMQLAATPNIGDYIPCLLGLDLQGLTRRIKATAKVFDDFFEKIIDEHI 252

Query: 95  RRREEGKNQNDFVGLMLDCINDKDLEVELDR 3
            + +E     D V +MLD +  ++ E  + R
Sbjct: 253 HKPKEEGQTKDLVDVMLDLMGSEETEYNIQR 283