BLASTX nr result

ID: Ophiopogon21_contig00042717 seq
BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon21_contig00042717
         (451 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EXX68115.1| oxidoreductase [Rhizophagus irregularis DAOM 1971...   148   2e-33
gb|KNC99224.1| hypothetical protein SPPG_05480 [Spizellomyces pu...   107   5e-21
ref|XP_004357175.1| 11beta-hydroxysteroid dehydrogenase-like, pu...    86   1e-14
ref|WP_047511469.1| short-chain dehydrogenase [Methylibium sp. C...    78   2e-12
ref|WP_056811307.1| MULTISPECIES: short-chain dehydrogenase [Rhi...    77   7e-12
ref|WP_015029469.1| short-chain dehydrogenase [Emticicia oligotr...    75   2e-11
ref|WP_013453982.1| short-chain dehydrogenase [Marivirga tractuo...    75   2e-11
gb|KRO79147.1| short-chain dehydrogenase, partial [Polaribacter ...    74   3e-11
ref|WP_047588718.1| short-chain dehydrogenase [Methylibium sp. Y...    74   3e-11
ref|WP_008868051.1| short-chain dehydrogenase [Flavobacteria bac...    74   3e-11
ref|WP_047485874.1| short-chain dehydrogenase [Methylibium sp. C...    74   6e-11
ref|WP_052233781.1| short-chain dehydrogenase [Massilia sp. WF1]...    73   9e-11
ref|WP_008444711.1| 11-beta-hydroxysteroid dehydrogenase [Janthi...    72   1e-10
ref|WP_019560422.1| SDR family oxidoreductase [Caldimonas mangan...    72   2e-10
ref|WP_043379493.1| short-chain dehydrogenase [Comamonas aquatica]     72   2e-10
gb|EXU81407.1| short-chain dehydrogenase [Comamonas aquatica DA1...    72   2e-10
ref|WP_057677240.1| short-chain dehydrogenase [Curvibacter sp. P...    71   3e-10
gb|KPK85018.1| short-chain dehydrogenase [Bacteroides sp. SM23_6...    71   3e-10
ref|WP_051933208.1| short-chain dehydrogenase [Massilia sp. BSC265]    71   3e-10
ref|WP_048439303.1| short-chain dehydrogenase [Caenimonas sp. SL...    71   3e-10

>gb|EXX68115.1| oxidoreductase [Rhizophagus irregularis DAOM 197198w]
          Length = 320

 Score =  148 bits (373), Expect = 2e-33
 Identities = 73/120 (60%), Positives = 89/120 (74%)
 Frame = -3

Query: 449 KKAEKXXXXXXXXXXXXXXXVPHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIV 270
           KKAEK               VP +TGYAASK AL+GFFESLQ+E+L  NIF+TIAYPGIV
Sbjct: 180 KKAEKPRIVVVSSLASVLPFVPMRTGYAASKCALRGFFESLQTELLKDNIFVTIAYPGIV 239

Query: 269 KTDIGQNRLGPNPVDMDYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFPD 90
           KT+I  +RLG NP  +D+  AMP EECAK+IVDGTI+A +EI+FT+L K GKI++G FPD
Sbjct: 240 KTEINDSRLGKNPKHLDFNKAMPAEECAKVIVDGTIRADREIVFTHLGKLGKILDGVFPD 299


>gb|KNC99224.1| hypothetical protein SPPG_05480 [Spizellomyces punctatus DAOM
           BR117]
          Length = 302

 Score =  107 bits (266), Expect = 5e-21
 Identities = 47/99 (47%), Positives = 68/99 (68%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPNPVDMDYTNA 207
           P +TGY A+K+A++GF+E+LQSE+ DQ +F+T+ YPG V T+I   RLG  P D+D   A
Sbjct: 185 PSRTGYCATKFAVRGFYEALQSELYDQGVFVTLVYPGAVVTNINATRLGSGPADLDMKGA 244

Query: 206 MPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFPD 90
           M  E CAK++V  T +  KE++ T+  K G++ EG  PD
Sbjct: 245 MTAETCAKLMVKATKQGRKELVMTHKFKLGRMWEGMVPD 283


>ref|XP_004357175.1| 11beta-hydroxysteroid dehydrogenase-like, putative [Acanthamoeba
           castellanii str. Neff] gi|440804142|gb|ELR25020.1|
           11beta-hydroxysteroid dehydrogenase-like, putative
           [Acanthamoeba castellanii str. Neff]
          Length = 305

 Score = 85.5 bits (210), Expect = 1e-14
 Identities = 42/100 (42%), Positives = 60/100 (60%), Gaps = 1/100 (1%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQN-IFITIAYPGIVKTDIGQNRLGPNPVDMDYTN 210
           PH++GY ASK+AL GF+E+L+ E+  +  I +T+  PG V T+I   RLGPNP   D + 
Sbjct: 186 PHRSGYCASKFALHGFYETLRQELSPKYPIGVTLVCPGFVNTNINATRLGPNPTTFDISK 245

Query: 209 AMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFPD 90
           AMP EE  +IIV       +E +FT   K G+ +    P+
Sbjct: 246 AMPVEEAGRIIVSAVRNGKREEVFTREGKIGEWVRFFLPE 285


>ref|WP_047511469.1| short-chain dehydrogenase [Methylibium sp. CF468]
          Length = 268

 Score = 78.2 bits (191), Expect = 2e-12
 Identities = 40/96 (41%), Positives = 61/96 (63%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T YAA+K+A+ GFFESL+ E+  Q I +TIAYPG+V T+I   G +  G       +
Sbjct: 148 PGRTAYAATKFAMTGFFESLRPEIGRQGISVTIAYPGVVATEIRRRGYDASGQAAGRSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
           D ++AMP E CA++I+DG     ++++ T   K G+
Sbjct: 208 DESDAMPVETCARLIIDGAEARRRDVVMTAQGKLGR 243


>ref|WP_056811307.1| MULTISPECIES: short-chain dehydrogenase [Rhizobacter]
           gi|945424487|gb|KQU80781.1| short-chain dehydrogenase
           [Rhizobacter sp. Root29] gi|945549189|gb|KQW04324.1|
           short-chain dehydrogenase [Rhizobacter sp. Root1238]
           gi|946061891|gb|KRB14544.1| short-chain dehydrogenase
           [Rhizobacter sp. Root16D2]
          Length = 268

 Score = 76.6 bits (187), Expect = 7e-12
 Identities = 39/96 (40%), Positives = 60/96 (62%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQN--RLGPNPV---DM 222
           P +T YAA+K+A+ GFFESL+ E+  Q I +TIAYPG+V T+I +     G  P     +
Sbjct: 148 PGRTAYAATKFAMTGFFESLRPEIGRQGISVTIAYPGVVATEIRRRGYDAGGQPAGRSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
           D + AMP E CA++I++G     ++++ T   K G+
Sbjct: 208 DESAAMPVETCARLIIEGAEARRRDVVMTAQGKLGR 243


>ref|WP_015029469.1| short-chain dehydrogenase [Emticicia oligotrophica]
           gi|387855676|gb|AFK03773.1| short-chain
           dehydrogenase/reductase SDR [Emticicia oligotrophica DSM
           17448]
          Length = 265

 Score = 74.7 bits (182), Expect = 2e-11
 Identities = 39/103 (37%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPN-----PVDM 222
           P ++GY ASK+AL GFF++L++EV D  I +T+  PG +KT+I  N +  +      +D 
Sbjct: 150 PMRSGYCASKHALHGFFDALRAEVFDDKIGVTLICPGYIKTNISLNAVAADGSKFGKMDE 209

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFP 93
           +  N MP EECA+ IVD   K   E+        G  ++  FP
Sbjct: 210 NQANGMPAEECARQIVDAIYKNKDEVYMGGKEVLGVYLKRFFP 252


>ref|WP_013453982.1| short-chain dehydrogenase [Marivirga tractuosa]
           gi|312942649|gb|ADR21839.1| short-chain
           dehydrogenase/reductase SDR [Marivirga tractuosa DSM
           4126]
          Length = 264

 Score = 74.7 bits (182), Expect = 2e-11
 Identities = 38/103 (36%), Positives = 60/103 (58%), Gaps = 5/103 (4%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPN-----PVDM 222
           P ++ YAA+K+AL GFF+SL++E+ + NI +T+  PG +KTD+  N LG +      +D 
Sbjct: 149 PWRSSYAAAKHALHGFFDSLRTELHNDNIKVTMVCPGFIKTDVSVNALGSDGKPTGEMDN 208

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFP 93
              N M  +ECAK I+    K   E +     +FG +++  FP
Sbjct: 209 AQANGMSAQECAKRIISAVEKDKLETVVGGTERFGVLLKRLFP 251


>gb|KRO79147.1| short-chain dehydrogenase, partial [Polaribacter sp. BACL8
           MAG-120531-bin13]
          Length = 187

 Score = 74.3 bits (181), Expect = 3e-11
 Identities = 38/103 (36%), Positives = 61/103 (59%), Gaps = 5/103 (4%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPNP-----VDM 222
           P+++GYAASK+AL GFF+SL++E+ D  I +++  PG VKT +  N LGPN      +D 
Sbjct: 73  PYRSGYAASKHALHGFFDSLRAELEDSGISVSLLAPGFVKTQVSVNALGPNGEAIGIMDQ 132

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFP 93
              N +  E+CAK I++  +K  K  ++     +   ++  FP
Sbjct: 133 AQANGISAEQCAKKIIN-ALKHGKREVYIGRESYAAYVKRYFP 174


>ref|WP_047588718.1| short-chain dehydrogenase [Methylibium sp. YR605]
          Length = 268

 Score = 74.3 bits (181), Expect = 3e-11
 Identities = 38/96 (39%), Positives = 60/96 (62%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRL----GP-NPVDM 222
           P +T YAA+K+A+ GFFESL+ E+  Q I +TIAYPG+V T+I +       GP     +
Sbjct: 148 PGRTAYAATKFAMTGFFESLRPEIGRQGISVTIAYPGVVATEIRRRGYDASGGPAGRSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
           D ++AMP   CA++I++G     ++++ T   K G+
Sbjct: 208 DESDAMPVGTCARLIIEGAEARRRDVVMTAQGKLGR 243


>ref|WP_008868051.1| short-chain dehydrogenase [Flavobacteria bacterium MS024-3C]
           gi|225004790|gb|EEG42748.1| short-chain
           dehydrogenase/reductase SDR [Flavobacteria bacterium
           MS024-3C] gi|949117889|gb|KRP00711.1| short-chain
           dehydrogenase [Polaribacter sp. BACL8 MAG-120619-bin41]
           gi|949135930|gb|KRP13169.1| short-chain dehydrogenase
           [Polaribacter sp. BACL8 MAG-120419-bin8]
          Length = 264

 Score = 74.3 bits (181), Expect = 3e-11
 Identities = 38/103 (36%), Positives = 61/103 (59%), Gaps = 5/103 (4%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPNP-----VDM 222
           P+++GYAASK+AL GFF+SL++E+ D  I +++  PG VKT +  N LGPN      +D 
Sbjct: 150 PYRSGYAASKHALHGFFDSLRAELEDSGISVSLLAPGFVKTQVSVNALGPNGEAIGIMDQ 209

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIEGSFP 93
              N +  E+CAK I++  +K  K  ++     +   ++  FP
Sbjct: 210 AQANGISAEQCAKKIIN-ALKHGKREVYIGRESYAAYVKRYFP 251


>ref|WP_047485874.1| short-chain dehydrogenase [Methylibium sp. CF059]
          Length = 268

 Score = 73.6 bits (179), Expect = 6e-11
 Identities = 38/99 (38%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y  +K+A+ GFFE+L+ E++   + +T+AYPG+V T+I   G N  G       +
Sbjct: 148 PGRTAYCGAKFAMTGFFEALRVELVSAGVSVTLAYPGVVATEIRYRGFNARGEAAGKSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIE 105
           D + AM  E CA++IVDGT    ++I+ T   K G+ I+
Sbjct: 208 DESGAMSVETCARLIVDGTEARTRDIVMTTKGKLGRWIK 246


>ref|WP_052233781.1| short-chain dehydrogenase [Massilia sp. WF1]
           gi|914249845|gb|KNZ67766.1| short-chain dehydrogenase
           [Massilia sp. WF1] gi|941929216|gb|ALK97655.1|
           short-chain dehydrogenase [Massilia sp. WG5]
          Length = 267

 Score = 72.8 bits (177), Expect = 9e-11
 Identities = 38/99 (38%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y+ASK+A+ GFFE+L++E+    + +TIAYPG+V T I   G N LG       +
Sbjct: 148 PGRTAYSASKFAMTGFFEALRAELKGAGVSVTIAYPGVVDTRIRYRGFNALGREAGSSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIE 105
               AM   ECA++I DG  +  +E++ T   K G++++
Sbjct: 208 REEGAMTVRECARLIADGMERRKREVVMTAKGKLGRLMK 246


>ref|WP_008444711.1| 11-beta-hydroxysteroid dehydrogenase [Janthinobacterium sp. HH01]
           gi|444790787|gb|ELX12334.1| 11-beta-hydroxysteroid
           dehydrogenase [Janthinobacterium sp. HH01]
          Length = 268

 Score = 72.4 bits (176), Expect = 1e-10
 Identities = 40/98 (40%), Positives = 59/98 (60%), Gaps = 7/98 (7%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQN----RLGPNPV 228
           P +T Y ASK+A+ GFFE+L++E+    + +TIAYPG+V T I   G N     LG + +
Sbjct: 148 PGRTAYGASKFAMSGFFEALRAELKAAGVSVTIAYPGVVATQIRYRGYNAAGGELGSSSL 207

Query: 227 DMDYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
             D   AM  EECA++IVDG     +E++ +   K G+
Sbjct: 208 KED--KAMTVEECARLIVDGMQARRREVVMSAKGKLGR 243


>ref|WP_019560422.1| SDR family oxidoreductase [Caldimonas manganoxidans]
          Length = 268

 Score = 72.0 bits (175), Expect = 2e-10
 Identities = 36/96 (37%), Positives = 62/96 (64%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG-PNPVD-M 222
           P +T Y+A+K+A+ GFFE+L+ E++   + +TIAYPG+V T+I   G N  G P  V  +
Sbjct: 148 PGRTAYSATKFAMTGFFEALRVELIGTGVSVTIAYPGVVATEIRRRGFNAAGQPAGVSGL 207

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
           D   AMP + CA++I++G  +  ++++ T   + G+
Sbjct: 208 DERGAMPVDTCARLILEGLERRRRDVVMTAQGRLGR 243


>ref|WP_043379493.1| short-chain dehydrogenase [Comamonas aquatica]
          Length = 270

 Score = 71.6 bits (174), Expect = 2e-10
 Identities = 37/96 (38%), Positives = 57/96 (59%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y+ASK+A+ GFFE+L++E+  Q + +T+AYPG+V T I   G N  G       +
Sbjct: 150 PGRTAYSASKFAMNGFFEALRAELKPQGVSVTLAYPGVVDTRIRYHGYNARGEAAGRSGL 209

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
               AMP  ECA++I+ G     +E++ T   K G+
Sbjct: 210 KEDGAMPVAECARLILAGMQHRQREVVMTTRGKLGR 245


>gb|EXU81407.1| short-chain dehydrogenase [Comamonas aquatica DA1877]
          Length = 266

 Score = 71.6 bits (174), Expect = 2e-10
 Identities = 37/96 (38%), Positives = 57/96 (59%), Gaps = 5/96 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y+ASK+A+ GFFE+L++E+  Q + +T+AYPG+V T I   G N  G       +
Sbjct: 146 PGRTAYSASKFAMNGFFEALRAELKPQGVSVTLAYPGVVDTRIRYHGYNARGEAAGRSGL 205

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGK 114
               AMP  ECA++I+ G     +E++ T   K G+
Sbjct: 206 KEDGAMPVAECARLILAGMQHRQREVVMTTRGKLGR 241


>ref|WP_057677240.1| short-chain dehydrogenase [Curvibacter sp. PAE-UM]
           gi|948296695|gb|KRI01319.1| short-chain dehydrogenase
           [Curvibacter sp. PAE-UM]
          Length = 270

 Score = 71.2 bits (173), Expect = 3e-10
 Identities = 36/99 (36%), Positives = 59/99 (59%), Gaps = 5/99 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y+ASK+A+ GFFE+L++E+    + +T AYPG+V T I   G N  G       +
Sbjct: 150 PGRTAYSASKFAMTGFFEALRAELKPAGVSVTTAYPGVVATQIRYRGLNAAGVAAGSSGL 209

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIE 105
              +AMP E CA++I+DG     +E++ +   K G+ ++
Sbjct: 210 KEDDAMPVEVCARLILDGMNARQREVVMSTKGKLGRYLK 248


>gb|KPK85018.1| short-chain dehydrogenase [Bacteroides sp. SM23_62_1]
          Length = 264

 Score = 71.2 bits (173), Expect = 3e-10
 Identities = 34/87 (39%), Positives = 56/87 (64%), Gaps = 5/87 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDIGQNRLGPN-----PVDM 222
           P ++ YAA+K+A+KG+FE++  E+ D+NIF+TIAYPG ++T+I    L  +      +D 
Sbjct: 149 PLRSAYAAAKHAVKGYFETVSIELRDRNIFVTIAYPGRIRTNISLRALTKDGKPQGVMDK 208

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEII 141
                +P E+CAKI V+   K  +E++
Sbjct: 209 GLAGGLPVEKCAKIYVNAIYKKRREVL 235


>ref|WP_051933208.1| short-chain dehydrogenase [Massilia sp. BSC265]
          Length = 252

 Score = 71.2 bits (173), Expect = 3e-10
 Identities = 37/99 (37%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLGPN--PVDM 222
           P +T Y+A+K+A+ GFFE+L++E+    + +T AYPG+V T I   G N  G +     +
Sbjct: 132 PGRTAYSATKFAMTGFFEALRAEMKGHGVSVTTAYPGVVATRIRYRGFNAQGESMGASAL 191

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIE 105
           +   AM  EECA++IV+G     +E++ T   K G+ I+
Sbjct: 192 NEEGAMSVEECARLIVEGMNARRREVVMTTKGKLGRFIK 230


>ref|WP_048439303.1| short-chain dehydrogenase [Caenimonas sp. SL110]
          Length = 270

 Score = 71.2 bits (173), Expect = 3e-10
 Identities = 36/99 (36%), Positives = 59/99 (59%), Gaps = 5/99 (5%)
 Frame = -3

Query: 386 PHKTGYAASKYALKGFFESLQSEVLDQNIFITIAYPGIVKTDI---GQNRLG--PNPVDM 222
           P +T Y+A+K+A+ GFFESL+ E+    + +T A+PG+V T I   G N  G       +
Sbjct: 150 PGRTAYSATKFAMTGFFESLRIEMKGAGVSVTTAFPGVVATQIRYRGYNAKGEPAGTSGL 209

Query: 221 DYTNAMPPEECAKIIVDGTIKAHKEIIFTNLAKFGKIIE 105
              NAM  +ECA++I+DG     +E++ T   K G++++
Sbjct: 210 KEDNAMSVQECARLIIDGMNARQREVVMTAQGKVGRLLK 248
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