BLASTX nr result
ID: Ophiopogon21_contig00038277
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon21_contig00038277
(620 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_009781494.1| PREDICTED: uncharacterized protein LOC104230... 69 3e-09
ref|XP_013726119.1| PREDICTED: uncharacterized protein LOC106429... 68 5e-09
ref|XP_010424837.1| PREDICTED: uncharacterized protein LOC104710... 67 8e-09
ref|XP_010667661.1| PREDICTED: uncharacterized protein LOC104884... 66 1e-08
ref|XP_009766185.1| PREDICTED: uncharacterized protein LOC104217... 66 1e-08
ref|XP_009781619.1| PREDICTED: uncharacterized protein LOC104230... 66 2e-08
ref|XP_009587473.1| PREDICTED: uncharacterized protein LOC104085... 65 2e-08
ref|XP_010507511.1| PREDICTED: uncharacterized protein LOC104784... 65 3e-08
ref|XP_010420757.1| PREDICTED: uncharacterized protein LOC104706... 65 3e-08
ref|XP_009769461.1| PREDICTED: uncharacterized protein LOC104220... 65 4e-08
ref|XP_009803597.1| PREDICTED: uncharacterized protein LOC104248... 65 4e-08
ref|XP_013601198.1| PREDICTED: uncharacterized protein LOC106308... 64 5e-08
ref|XP_013745885.1| PREDICTED: uncharacterized protein LOC106448... 64 7e-08
ref|XP_010541748.1| PREDICTED: uncharacterized protein LOC104815... 64 7e-08
ref|XP_013704886.1| PREDICTED: uncharacterized protein LOC106408... 64 9e-08
ref|XP_013706904.1| PREDICTED: uncharacterized protein LOC106410... 63 1e-07
ref|XP_008391159.1| PREDICTED: uncharacterized protein LOC103453... 63 1e-07
ref|XP_010521406.1| PREDICTED: LOW QUALITY PROTEIN: uncharacteri... 62 2e-07
ref|XP_013704720.1| PREDICTED: uncharacterized protein LOC106408... 62 3e-07
ref|XP_013650404.1| PREDICTED: uncharacterized protein LOC106354... 61 4e-07
>ref|XP_009781494.1| PREDICTED: uncharacterized protein LOC104230401 [Nicotiana
sylvestris]
Length = 149
Score = 68.6 bits (166), Expect = 3e-09
Identities = 48/129 (37%), Positives = 61/129 (47%), Gaps = 2/129 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIE-TRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLL 178
L T RL ++I +CVLCE IE T DHLFFECP+S L L +Q V+N
Sbjct: 7 LATVDRLAKWEIQVPQSCVLCERDIEETHDHLFFECPYSQSLWKGMLGWLRYQRSVANWE 66
Query: 179 CEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIHI 358
E + N +N + V+HIW ERN R FQNQ + I +HI
Sbjct: 67 AEVKWLSANANNRNPRKTILGVVFAAVVYHIWMERNDRRFQNQKREAKDRAKDITIQVHI 126
Query: 359 L-LRTCMWK 382
+ C WK
Sbjct: 127 TGQQKCKWK 135
>ref|XP_013726119.1| PREDICTED: uncharacterized protein LOC106429897 [Brassica napus]
Length = 267
Score = 67.8 bits (164), Expect = 5e-09
Identities = 37/100 (37%), Positives = 57/100 (57%), Gaps = 1/100 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*T-LCKLKLHFQTPVSNLL 178
LPT RL + +N A CVLC S +E+ DHLFF+C ++S + + C + PVS L
Sbjct: 121 LPTRDRLASWGMNIPAHCVLCSSGLESHDHLFFQCTYASSVWSRFCVGRYLSAPPVSTLA 180
Query: 179 CEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIF 298
C A F ++ ++ + + L V ++ +W+ERN RIF
Sbjct: 181 CANAMRCHQFTSRPRTIPVLKLILQVVIYCLWRERNTRIF 220
>ref|XP_010424837.1| PREDICTED: uncharacterized protein LOC104710005, partial [Camelina
sativa]
Length = 310
Score = 67.0 bits (162), Expect = 8e-09
Identities = 38/101 (37%), Positives = 53/101 (52%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
LPT RL + +N A+CVLC + IET +HLFF CPFS+ L K P++
Sbjct: 165 LPTRDRLRSWGLNIPASCVLCPNGIETHEHLFFTCPFSTNLWGFFAAKFFPNPPLTLSDI 224
Query: 182 EAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQN 304
A ++ K+ + + V+HIWKERN RIF +
Sbjct: 225 PAWILNLRQPHVAKAITIIKLLFQTVVYHIWKERNARIFSS 265
>ref|XP_010667661.1| PREDICTED: uncharacterized protein LOC104884674 [Beta vulgaris
subsp. vulgaris]
Length = 484
Score = 66.2 bits (160), Expect = 1e-08
Identities = 43/119 (36%), Positives = 62/119 (52%), Gaps = 2/119 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNL-- 175
L T R+L + +ATCVLC +A +TR HLF +C +S + C +HF T +L
Sbjct: 354 LITNDRMLQLGLLTDATCVLCNAAPKTRAHLFIQCNYSKNIWQSCMKVIHFPTSHYHLPE 413
Query: 176 LCEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYI 352
+ E A+ H + L +V+HIW +RN++IF NQ M VFR I +I
Sbjct: 414 IIEMASHHSR--GNRRKHHLYNMLFVESVYHIWLQRNKKIFDNQLMPPQQVFRVILFHI 470
>ref|XP_009766185.1| PREDICTED: uncharacterized protein LOC104217597 [Nicotiana
sylvestris]
Length = 177
Score = 66.2 bits (160), Expect = 1e-08
Identities = 46/138 (33%), Positives = 66/138 (47%), Gaps = 3/138 (2%)
Frame = +2
Query: 17 RLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQ-TPVSNLLCEAAA 193
RL + +N TC LC+ IET HLFFEC + T+ + LH+Q L +
Sbjct: 40 RLARWGVNIETTCALCQQGIETVQHLFFECEMTG---TIWQQLLHWQGIQRKKLKWQEEL 96
Query: 194 IHFNFIAKNKS--FILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIHILLR 367
+AK +S L R L V++IW+ERN IF+ + + R+I IH LR
Sbjct: 97 TWIQRVAKGRSGGAALCRMTLAAAVYYIWQERNNVIFKQEKRTAASIVRKIIQEIH--LR 154
Query: 368 TCMWKVSNQAFTLSNWGL 421
M+ + T +NW L
Sbjct: 155 ATMFPRLERTMTKANWYL 172
>ref|XP_009781619.1| PREDICTED: uncharacterized protein LOC104230493 [Nicotiana
sylvestris]
Length = 155
Score = 65.9 bits (159), Expect = 2e-08
Identities = 44/126 (34%), Positives = 60/126 (47%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
L T RL + + D C LC A ET DHLFF C +SS + + +
Sbjct: 18 LSTRERLASWGVTDTLVCPLCNVANETIDHLFFSCVYSSGIWNILLQWQGLTRKTMSWQH 77
Query: 182 EAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIHIL 361
E A + N ++ +SR A+ V+HIW+ERN RIFQN+ + V R+I I
Sbjct: 78 EMAWMEVNERGRSARAEVSRMAIAGCVYHIWQERNMRIFQNKQRQEEQVIRQIIQ--EIF 135
Query: 362 LRTCMW 379
R MW
Sbjct: 136 CRGSMW 141
>ref|XP_009587473.1| PREDICTED: uncharacterized protein LOC104085197, partial [Nicotiana
tomentosiformis]
Length = 177
Score = 65.5 bits (158), Expect = 2e-08
Identities = 46/129 (35%), Positives = 61/129 (47%), Gaps = 2/129 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIE-TRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLL 178
L T RL +KI + +CVLCE IE T DHLFF+CP+S L L +Q V+N
Sbjct: 7 LATVDRLAKWKIQVSQSCVLCERDIEETHDHLFFKCPYSQSLWKGMLGWLRYQRSVANWE 66
Query: 179 CEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIHI 358
+ N +N + V+HIW +RN R FQNQ + I +HI
Sbjct: 67 AGVKWLSVNANNRNPRKTILGVVFAAAVYHIWMKRNDRRFQNQKREAKDRAKDIPIQVHI 126
Query: 359 L-LRTCMWK 382
+ C WK
Sbjct: 127 TGQQKCKWK 135
>ref|XP_010507511.1| PREDICTED: uncharacterized protein LOC104784146 [Camelina sativa]
Length = 1269
Score = 65.1 bits (157), Expect = 3e-08
Identities = 40/102 (39%), Positives = 54/102 (52%), Gaps = 1/102 (0%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
LPT RL + +N +CVLC S +E+ HLFFECPF+S L K P NL
Sbjct: 1056 LPTRDRLHRWGMNVPTSCVLCSSGVESHAHLFFECPFASNLWGFLAAKF-ISNPPCNLPA 1114
Query: 182 EAAAI-HFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQN 304
A+ I N ++ + + L ++H+WKERN RIF N
Sbjct: 1115 AASWIASRNSPSQANLTAILKLLLTTILYHVWKERNSRIFAN 1156
>ref|XP_010420757.1| PREDICTED: uncharacterized protein LOC104706278 [Camelina sativa]
Length = 283
Score = 65.1 bits (157), Expect = 3e-08
Identities = 39/103 (37%), Positives = 54/103 (52%), Gaps = 2/103 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
LPT RL + +N A+CVLC + IET +HLFF CPFS+ L K P++ L
Sbjct: 138 LPTRDRLRSWGLNIPASCVLCPNGIETHEHLFFTCPFSTNLWGFFAAKFFPNPPLT--LS 195
Query: 182 EAAAIHFNFIAKN--KSFILSRAALGVTVWHIWKERNRRIFQN 304
+ A N + K+ + ++HIWKERN RIF +
Sbjct: 196 DIPAWILNLCQSHVAKAITSIKLLFQTVIYHIWKERNARIFSS 238
>ref|XP_009769461.1| PREDICTED: uncharacterized protein LOC104220316, partial [Nicotiana
sylvestris]
Length = 123
Score = 64.7 bits (156), Expect = 4e-08
Identities = 42/103 (40%), Positives = 51/103 (49%), Gaps = 1/103 (0%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIE-TRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLL 178
L T RL + I +CVLC IE T DHL+FECP+S L T L +Q V N
Sbjct: 7 LATVDRLANWGIQVTHSCVLCGGDIEETHDHLYFECPYSQSLWTGMLEWLSYQRKVENWE 66
Query: 179 CEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ 307
E + + +N L V V+HIW ERN R FQNQ
Sbjct: 67 DEVQWLSTDVNNRNPRKTLLGVVFAVVVYHIWMERNERRFQNQ 109
>ref|XP_009803597.1| PREDICTED: uncharacterized protein LOC104248941 [Nicotiana
sylvestris]
Length = 149
Score = 64.7 bits (156), Expect = 4e-08
Identities = 47/128 (36%), Positives = 59/128 (46%), Gaps = 2/128 (1%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIE-TRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLL 178
L T RL + I+ +CVLC IE T DHL+FECP+S L T L +Q V N
Sbjct: 7 LATVDRLAKWGIHVTHSCVLCGGDIEETHDHLYFECPYSQSLWTGMLEWLSYQRKVENWE 66
Query: 179 CEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIHI 358
E + N +N L V+HIW ERN R FQNQ + I +HI
Sbjct: 67 DEVQWLTTNVNNRNPRKTLLGVVFAAVVYHIWIERNERRFQNQRREVKDRAKDIVMQVHI 126
Query: 359 L-LRTCMW 379
+ C W
Sbjct: 127 KGQKKCKW 134
>ref|XP_013601198.1| PREDICTED: uncharacterized protein LOC106308597 [Brassica oleracea
var. oleracea]
Length = 308
Score = 64.3 bits (155), Expect = 5e-08
Identities = 42/118 (35%), Positives = 60/118 (50%), Gaps = 5/118 (4%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSS--YL*TLCKLKLHFQTPVSNL 175
LPT SRL + IN + C+LC SA+E RDHLF CP S + L +L H +
Sbjct: 169 LPTKSRLAAWAINISTACILCNSAMEDRDHLFLRCPTSEVIWACVLRRLGAHHHS----- 223
Query: 176 LCEAAAIHFNFIAKNKS---FILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRI 340
++++ N S +L R A T+++IW ERNRRI N ++F+ I
Sbjct: 224 -FHTWTAMMDWLSLNDSRTITLLKRLASQATIYNIWTERNRRIHDNVITPPAMIFKTI 280
>ref|XP_013745885.1| PREDICTED: uncharacterized protein LOC106448564 [Brassica napus]
Length = 1482
Score = 63.9 bits (154), Expect = 7e-08
Identities = 42/118 (35%), Positives = 60/118 (50%), Gaps = 5/118 (4%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSS--YL*TLCKLKLHFQTPVSNL 175
LPT SRL + IN + C+LC SA+E RDHLF CP S + L +L H +
Sbjct: 1343 LPTKSRLAAWGINISTACILCNSAMEDRDHLFLRCPTSKVIWACVLRRLGAHHHS----- 1397
Query: 176 LCEAAAIHFNFIAKNKS---FILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRI 340
++++ N S +L R A T+++IW ERNRRI N ++F+ I
Sbjct: 1398 -FHTWTAMMDWLSLNDSRTITLLKRLASQATIYNIWTERNRRIHDNVITPPAMIFKTI 1454
>ref|XP_010541748.1| PREDICTED: uncharacterized protein LOC104815125 [Tarenaya
hassleriana]
Length = 296
Score = 63.9 bits (154), Expect = 7e-08
Identities = 46/139 (33%), Positives = 63/139 (45%), Gaps = 1/139 (0%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
+PT RLL + I N+TCVLC E+ DHLFF+C FS+ K+ P + +C
Sbjct: 149 MPTRDRLLRWNIIQNSTCVLCNEEEESHDHLFFQCRFSAETWQELASKIWRNPPANLSMC 208
Query: 182 EAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIH-I 358
+ K IL + + V+ +WKERN RIF N V+ RI +
Sbjct: 209 LSWMEAIPQSDGLKRNILLKILIQHAVYEVWKERNSRIFSNVFATSHVMRSRIDQSVRTT 268
Query: 359 LLRTCMWKVSNQAFTLSNW 415
LL + S L NW
Sbjct: 269 LLSLQQVRESESNILLQNW 287
>ref|XP_013704886.1| PREDICTED: uncharacterized protein LOC106408720 [Brassica napus]
Length = 203
Score = 63.5 bits (153), Expect = 9e-08
Identities = 41/118 (34%), Positives = 60/118 (50%), Gaps = 5/118 (4%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSS--YL*TLCKLKLHFQTPVSNL 175
LPT SRL + IN + C+LC SA+E RDHLF CP S + L +L H +
Sbjct: 64 LPTKSRLAAWGINISTACILCNSAMEDRDHLFLRCPTSEVIWACVLRRLGAHHHS----- 118
Query: 176 LCEAAAIHFNFIAKNKS---FILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRI 340
++++ N S +L R A T+++IW ERNR+I N ++F+ I
Sbjct: 119 -FHTWTAMMDWLSLNDSRTIILLKRLASQATIYNIWTERNRKIHDNVITPPAMIFKTI 175
>ref|XP_013706904.1| PREDICTED: uncharacterized protein LOC106410831 [Brassica napus]
Length = 1117
Score = 63.2 bits (152), Expect = 1e-07
Identities = 36/101 (35%), Positives = 59/101 (58%)
Frame = +2
Query: 5 PT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLCE 184
PT R++ + I +++C+LC + +E+RDHL+FECP++ L T K +++ P N L E
Sbjct: 979 PTKDRMISWGIQIDSSCILCNAPLESRDHLYFECPYAWSLWTELSRKANWR-PSRNWLTE 1037
Query: 185 AAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ 307
+ + K+ +L A VT++ IW ERN RI + Q
Sbjct: 1038 LGLMQAMTLPKHDR-LLVLLAWQVTIYLIWTERNSRIHRQQ 1077
>ref|XP_008391159.1| PREDICTED: uncharacterized protein LOC103453396 [Malus domestica]
Length = 1219
Score = 63.2 bits (152), Expect = 1e-07
Identities = 45/143 (31%), Positives = 67/143 (46%), Gaps = 3/143 (2%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL--*TLCKLKLHFQTPVSNL 175
L T R+ F N CVLC+S +E+ HLFF+CPF+ L L + + + P
Sbjct: 1076 LTTLDRIGQFSSRANLNCVLCDSDLESHSHLFFDCPFTEGLWQHVLMRCGVPWLHPPWPS 1135
Query: 176 LCEAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRIYGYIH 355
++ A H+ K+ ++ + +L VTV+ IW ERN R+F N + + I I
Sbjct: 1136 FVDSVATHWR--GKSLPVVVKKLSLAVTVYCIWCERNNRMFNNCRKTSAALLQSITNMIW 1193
Query: 356 ILLRTCMWKVSN-QAFTLSNWGL 421
L T K S L NW +
Sbjct: 1194 CRLCTLHVKPSTINMMILYNWNV 1216
>ref|XP_010521406.1| PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC104800308
[Tarenaya hassleriana]
Length = 1816
Score = 62.4 bits (150), Expect = 2e-07
Identities = 37/102 (36%), Positives = 54/102 (52%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
LPT RLL + I +A C+LCE A E+ HLFF CP++S L + P LC
Sbjct: 1218 LPTKDRLLNWGIQVDAICILCEEAQESHTHLFFACPYASALWNHYVGRCWPSPPADIQLC 1277
Query: 182 EAAAIHFNFIAKNKSFILSRAALGVTVWHIWKERNRRIFQNQ 307
+ ++N+ ++ R + V V+ +W+ERN RIF Q
Sbjct: 1278 TSWLKGQADTSRNRC-LIGRLIIQVIVYEVWRERNARIFTGQ 1318
>ref|XP_013704720.1| PREDICTED: uncharacterized protein LOC106408510 [Brassica napus]
Length = 232
Score = 62.0 bits (149), Expect = 3e-07
Identities = 40/118 (33%), Positives = 61/118 (51%), Gaps = 5/118 (4%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSS--YL*TLCKLKLHFQTPVSNL 175
LPT SRL + IN + C+LC SA+E R+H+F CP S + L +L H +
Sbjct: 93 LPTKSRLAAWGINISTACILCNSAMEDRNHMFLRCPTSEVIWACVLRRLGAHHHS----- 147
Query: 176 LCEAAAIHFNFIAKNKS---FILSRAALGVTVWHIWKERNRRIFQNQ*MHKVVVFRRI 340
++++ N S +L R A T+++IW ERNRRI N + ++F+ I
Sbjct: 148 -FHTWTTMMDWLSLNDSRTITLLKRLASQATIYNIWTERNRRIHDNVIIPPAMIFKTI 204
>ref|XP_013650404.1| PREDICTED: uncharacterized protein LOC106354909 [Brassica napus]
gi|923823111|ref|XP_013694977.1| PREDICTED:
uncharacterized protein LOC106399045 [Brassica napus]
Length = 1651
Score = 61.2 bits (147), Expect = 4e-07
Identities = 37/103 (35%), Positives = 59/103 (57%), Gaps = 1/103 (0%)
Frame = +2
Query: 2 LPT*SRLL*FKINDNATCVLCESAIETRDHLFFECPFSSYL*TLCKLKLHFQTPVSNLLC 181
LPT +RL + + +C LC + E+RDHLF ECPFS + KL +P+ +
Sbjct: 1514 LPTRNRLAAWGMLTPTSCCLCSLSDESRDHLFIECPFSKDIWRRMLQKLFPTSPMFD--S 1571
Query: 182 EAAAIHFNFIAKNKSF-ILSRAALGVTVWHIWKERNRRIFQNQ 307
A +++ +A N+S L A+ V ++++W ERN R+F+NQ
Sbjct: 1572 WAQMLNWPSLAPNQSMRTLRGIAVQVIIYNLWTERNNRVFRNQ 1614