BLASTX nr result
ID: Ophiopogon21_contig00024138
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon21_contig00024138
(515 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AJF38260.1| glycosyltransferase family 47 IRX10 [Asparagus of... 198 2e-48
ref|XP_010066467.1| PREDICTED: probable beta-1,4-xylosyltransfer... 192 8e-47
gb|KMZ68407.1| pectin beta-glucuronyltransferase, family GT47 [Z... 191 2e-46
ref|XP_008777353.1| PREDICTED: probable beta-1,4-xylosyltransfer... 190 4e-46
ref|XP_002511079.1| catalytic, putative [Ricinus communis] gi|22... 190 4e-46
ref|XP_007038168.1| Exostosin family protein [Theobroma cacao] g... 190 4e-46
ref|XP_010909287.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 5e-46
ref|XP_006346627.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 5e-46
gb|KMZ68405.1| pectin beta-glucuronyltransferase, family GT47 [Z... 189 7e-46
ref|XP_010250506.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 7e-46
ref|XP_010036844.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 7e-46
gb|KJB53296.1| hypothetical protein B456_008G211000 [Gossypium r... 189 9e-46
ref|XP_012439067.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 9e-46
ref|XP_010663815.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 9e-46
ref|XP_009109804.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 9e-46
ref|XP_002284685.1| PREDICTED: probable beta-1,4-xylosyltransfer... 189 9e-46
ref|XP_012479636.1| PREDICTED: probable beta-1,4-xylosyltransfer... 188 1e-45
gb|KHG25925.1| putative beta-1,4-xylosyltransferase IRX10 -like ... 188 1e-45
ref|XP_010249251.1| PREDICTED: probable beta-1,4-xylosyltransfer... 188 1e-45
ref|XP_007043772.1| Exostosin family protein [Theobroma cacao] g... 188 1e-45
>gb|AJF38260.1| glycosyltransferase family 47 IRX10 [Asparagus officinalis]
Length = 416
Score = 198 bits (503), Expect = 2e-48
Identities = 95/100 (95%), Positives = 98/100 (98%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWE+IGVFVAEKDVP LDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA
Sbjct: 317 ADAIPWEDIGVFVAEKDVPNLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 376
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH ESLYL+PGEK+LNWTAGPVGDLKPW
Sbjct: 377 FHQVLNGLARKLPHDESLYLKPGEKLLNWTAGPVGDLKPW 416
>ref|XP_010066467.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10 [Eucalyptus
grandis] gi|629098585|gb|KCW64350.1| hypothetical
protein EUGRSUZ_G01977 [Eucalyptus grandis]
Length = 413
Score = 192 bits (488), Expect = 8e-47
Identities = 91/100 (91%), Positives = 97/100 (97%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVP LDTILTSISP+VILRKQRLLANPSMK+AMLFPQPAQSGDA
Sbjct: 314 ADAIPWEEIGVFVAEEDVPNLDTILTSISPEVILRKQRLLANPSMKRAMLFPQPAQSGDA 373
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH S+YL+PGEKVLNWTAGPVGDLKPW
Sbjct: 374 FHQILNGLARKLPHHRSVYLKPGEKVLNWTAGPVGDLKPW 413
>gb|KMZ68407.1| pectin beta-glucuronyltransferase, family GT47 [Zostera marina]
Length = 417
Score = 191 bits (485), Expect = 2e-46
Identities = 90/100 (90%), Positives = 96/100 (96%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV+E+DVPKLDTILTSISP+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 318 ADAIPWEEIGVFVSEEDVPKLDTILTSISPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 377
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH +YL+PGEKVLNWTAGPVGDLKPW
Sbjct: 378 FHQILNGLARKLPHGRDVYLKPGEKVLNWTAGPVGDLKPW 417
>ref|XP_008777353.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L [Phoenix
dactylifera]
Length = 416
Score = 190 bits (482), Expect = 4e-46
Identities = 88/100 (88%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWE+IGVFVAEKDVPKLDTILTSI P+V+LRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 317 ADAIPWEDIGVFVAEKDVPKLDTILTSIPPEVVLRKQRLLANPSMKQAMLFPQPAQPGDA 376
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH + YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 377 FHQILNGLARKLPHPRNAYLKPGEKILNWTAGPVGDLKPW 416
>ref|XP_002511079.1| catalytic, putative [Ricinus communis] gi|223550194|gb|EEF51681.1|
catalytic, putative [Ricinus communis]
Length = 417
Score = 190 bits (482), Expect = 4e-46
Identities = 90/100 (90%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV EKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ+GDA
Sbjct: 318 ADAIPWEEIGVFVDEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQAGDA 377
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH S+YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 378 FHQVLNGLARKLPHDRSVYLKPGEKILNWTAGPVGDLKPW 417
>ref|XP_007038168.1| Exostosin family protein [Theobroma cacao]
gi|508775413|gb|EOY22669.1| Exostosin family protein
[Theobroma cacao]
Length = 440
Score = 190 bits (482), Expect = 4e-46
Identities = 91/100 (91%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV EKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 341 ADAIPWEEIGVFVDEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 400
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH +S+YL+PGEKVLNWTAGPVGDLKPW
Sbjct: 401 FHQVLNGLARKLPHDKSVYLKPGEKVLNWTAGPVGDLKPW 440
>ref|XP_010909287.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L [Elaeis
guineensis]
Length = 416
Score = 189 bits (481), Expect = 5e-46
Identities = 90/100 (90%), Positives = 94/100 (94%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAEKDVPKLDTILTSI PDVILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 317 ADAIPWEEIGVFVAEKDVPKLDTILTSIPPDVILRKQRLLANPSMKQAMLFPQPAQPGDA 376
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH + YL+ GEK+LNWTAGPVGDLKPW
Sbjct: 377 FHQILNGLARKLPHPRNAYLKRGEKILNWTAGPVGDLKPW 416
>ref|XP_006346627.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L-like
[Solanum tuberosum]
Length = 416
Score = 189 bits (481), Expect = 5e-46
Identities = 90/100 (90%), Positives = 94/100 (94%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAEKDVP LDTILTSI P+ ILRKQRLLANPSMKQAMLFPQPAQSGDA
Sbjct: 317 ADAIPWEEIGVFVAEKDVPNLDTILTSIPPEEILRKQRLLANPSMKQAMLFPQPAQSGDA 376
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH S+YL PGEK+LNWTAGPVGDLKPW
Sbjct: 377 FHQILNGLARKLPHDRSVYLTPGEKILNWTAGPVGDLKPW 416
>gb|KMZ68405.1| pectin beta-glucuronyltransferase, family GT47 [Zostera marina]
Length = 417
Score = 189 bits (480), Expect = 7e-46
Identities = 88/100 (88%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV+E+DVPKLDTILTSISP+VILRKQRLLANPSMKQAMLFPQP Q GDA
Sbjct: 318 ADAIPWEEIGVFVSEEDVPKLDTILTSISPEVILRKQRLLANPSMKQAMLFPQPVQPGDA 377
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH +YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 378 FHQILNGLARKLPHGRDVYLKPGEKILNWTAGPVGDLKPW 417
>ref|XP_010250506.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L [Nelumbo
nucifera]
Length = 418
Score = 189 bits (480), Expect = 7e-46
Identities = 89/100 (89%), Positives = 94/100 (94%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAEKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 319 ADAIPWEEIGVFVAEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 378
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH + YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 379 FHQILNGLARKLPHGKGTYLKPGEKILNWTAGPVGDLKPW 418
>ref|XP_010036844.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L [Eucalyptus
grandis] gi|629082061|gb|KCW48506.1| hypothetical
protein EUGRSUZ_K02191 [Eucalyptus grandis]
Length = 419
Score = 189 bits (480), Expect = 7e-46
Identities = 90/100 (90%), Positives = 94/100 (94%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV EKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 320 ADAIPWEEIGVFVDEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 379
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH S+YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 380 FHQVLNGLARKLPHGRSVYLKPGEKILNWTAGPVGDLKPW 419
>gb|KJB53296.1| hypothetical protein B456_008G211000 [Gossypium raimondii]
Length = 255
Score = 189 bits (479), Expect = 9e-46
Identities = 90/100 (90%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV EKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 156 ADAIPWEEIGVFVDEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 215
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH +S+YL+PGE+VLNWTAGPVGDLKPW
Sbjct: 216 FHQVLNGLARKLPHDKSVYLKPGERVLNWTAGPVGDLKPW 255
>ref|XP_012439067.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L [Gossypium
raimondii] gi|763786224|gb|KJB53295.1| hypothetical
protein B456_008G211000 [Gossypium raimondii]
Length = 417
Score = 189 bits (479), Expect = 9e-46
Identities = 90/100 (90%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFV EKDVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 318 ADAIPWEEIGVFVDEKDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 377
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQVLNGLARKLPH +S+YL+PGE+VLNWTAGPVGDLKPW
Sbjct: 378 FHQVLNGLARKLPHDKSVYLKPGERVLNWTAGPVGDLKPW 417
>ref|XP_010663815.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L isoform X2
[Vitis vinifera]
Length = 341
Score = 189 bits (479), Expect = 9e-46
Identities = 88/100 (88%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 242 ADAIPWEEIGVFVAEEDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 301
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH ++YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 302 FHQILNGLARKLPHGRNIYLKPGEKILNWTAGPVGDLKPW 341
>ref|XP_009109804.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10 [Brassica
rapa]
Length = 411
Score = 189 bits (479), Expect = 9e-46
Identities = 91/100 (91%), Positives = 94/100 (94%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAEKDVPKLDTILTSI +VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 312 ADAIPWEEIGVFVAEKDVPKLDTILTSIPTEVILRKQRLLANPSMKQAMLFPQPAQPGDA 371
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH S+YLR GEKVLNWTAGPVGDLKPW
Sbjct: 372 FHQILNGLARKLPHDRSIYLREGEKVLNWTAGPVGDLKPW 411
>ref|XP_002284685.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10L isoform X1
[Vitis vinifera] gi|147860849|emb|CAN83159.1|
hypothetical protein VITISV_022554 [Vitis vinifera]
gi|297734521|emb|CBI15768.3| unnamed protein product
[Vitis vinifera]
Length = 416
Score = 189 bits (479), Expect = 9e-46
Identities = 88/100 (88%), Positives = 95/100 (95%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVP LDTILTSI P+VILRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 317 ADAIPWEEIGVFVAEEDVPNLDTILTSIPPEVILRKQRLLANPSMKQAMLFPQPAQPGDA 376
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH ++YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 377 FHQILNGLARKLPHGRNIYLKPGEKILNWTAGPVGDLKPW 416
>ref|XP_012479636.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10 [Gossypium
raimondii] gi|763764356|gb|KJB31610.1| hypothetical
protein B456_005G197500 [Gossypium raimondii]
Length = 412
Score = 188 bits (478), Expect = 1e-45
Identities = 88/100 (88%), Positives = 96/100 (96%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVPKLDTILTSI P+VILRKQRLLANPSMK+AMLFPQPA GDA
Sbjct: 313 ADAIPWEEIGVFVAEEDVPKLDTILTSIPPEVILRKQRLLANPSMKRAMLFPQPALPGDA 372
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH +S+YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 373 FHQILNGLARKLPHQKSIYLKPGEKILNWTAGPVGDLKPW 412
>gb|KHG25925.1| putative beta-1,4-xylosyltransferase IRX10 -like protein [Gossypium
arboreum]
Length = 426
Score = 188 bits (478), Expect = 1e-45
Identities = 88/100 (88%), Positives = 96/100 (96%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVPKLDTILTSI P+VILRKQRLLANPSMK+AMLFPQPA GDA
Sbjct: 327 ADAIPWEEIGVFVAEEDVPKLDTILTSIPPEVILRKQRLLANPSMKRAMLFPQPALPGDA 386
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH +S+YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 387 FHQILNGLARKLPHQKSIYLKPGEKILNWTAGPVGDLKPW 426
>ref|XP_010249251.1| PREDICTED: probable beta-1,4-xylosyltransferase IRX10 [Nelumbo
nucifera]
Length = 418
Score = 188 bits (478), Expect = 1e-45
Identities = 88/100 (88%), Positives = 96/100 (96%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAEKDVPKLDTILTSI P+V+LRKQRLLANPSMKQAMLFPQPAQ GDA
Sbjct: 319 ADAIPWEEIGVFVAEKDVPKLDTILTSIPPEVVLRKQRLLANPSMKQAMLFPQPAQPGDA 378
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLP +S++L+PGEK+LNWTAGPVGDLKPW
Sbjct: 379 FHQILNGLARKLPRDKSIHLKPGEKILNWTAGPVGDLKPW 418
>ref|XP_007043772.1| Exostosin family protein [Theobroma cacao]
gi|508707707|gb|EOX99603.1| Exostosin family protein
[Theobroma cacao]
Length = 412
Score = 188 bits (478), Expect = 1e-45
Identities = 88/100 (88%), Positives = 96/100 (96%)
Frame = +1
Query: 1 ADAIPWEEIGVFVAEKDVPKLDTILTSISPDVILRKQRLLANPSMKQAMLFPQPAQSGDA 180
ADAIPWEEIGVFVAE+DVPKLD ILTSI P+VILRKQRLLANPSMK+AMLFPQPAQ GDA
Sbjct: 313 ADAIPWEEIGVFVAEEDVPKLDAILTSIPPEVILRKQRLLANPSMKRAMLFPQPAQPGDA 372
Query: 181 FHQVLNGLARKLPHVESLYLRPGEKVLNWTAGPVGDLKPW 300
FHQ+LNGLARKLPH +S+YL+PGEK+LNWTAGPVGDLKPW
Sbjct: 373 FHQILNGLARKLPHQKSIYLKPGEKMLNWTAGPVGDLKPW 412