BLASTX nr result
ID: Mentha28_contig00017252
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha28_contig00017252
(2004 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|J7LMP2.1|TPS5_PHYDL RecName: Full=Bicyclogermacrene synthase;... 253 7e-91
sp|J7LP58.1|TPS1_PHYDL RecName: Full=Bifunctional sesquiterpene ... 253 5e-89
sp|Q49SP3.1|TPSPS_POGCB RecName: Full=Patchoulol synthase; Short... 232 5e-80
sp|Q49SP6.1|TPGD2_POGCB RecName: Full=Germacrene D synthase 2; A... 229 2e-77
sp|Q49SP4.1|TPGD1_POGCB RecName: Full=Germacrene D synthase 1; A... 215 2e-76
gb|AHL24448.1| patchoulol synthase variant [Pogostemon cablin] 221 5e-76
ref|XP_002282488.1| PREDICTED: (-)-germacrene D synthase [Vitis ... 206 2e-75
emb|CAN81129.1| hypothetical protein VITISV_002911 [Vitis vinifera] 204 1e-74
emb|CBI20475.3| unnamed protein product [Vitis vinifera] 202 2e-74
ref|XP_002283076.1| PREDICTED: (-)-germacrene D synthase [Vitis ... 202 2e-74
emb|CBI20288.3| unnamed protein product [Vitis vinifera] 207 2e-74
ref|XP_003634645.1| PREDICTED: (-)-germacrene D synthase-like [V... 206 4e-74
emb|CBI20287.3| unnamed protein product [Vitis vinifera] 206 4e-74
ref|XP_007021122.1| Delta-cadinene synthase isozyme A, putative ... 209 6e-74
ref|XP_002283070.1| PREDICTED: (-)-germacrene D synthase [Vitis ... 200 6e-74
ref|XP_007021115.1| Delta-cadinene synthase isozyme A, putative ... 208 2e-73
gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa] 203 2e-72
ref|XP_004288818.1| PREDICTED: (-)-germacrene D synthase-like [F... 201 5e-72
ref|XP_002283344.1| PREDICTED: (-)-germacrene D synthase [Vitis ... 196 1e-71
emb|CBI31305.3| unnamed protein product [Vitis vinifera] 196 5e-71
>sp|J7LMP2.1|TPS5_PHYDL RecName: Full=Bicyclogermacrene synthase; AltName: Full=Terpene
synthase 5; Short=LdTPS5 gi|403084370|gb|AFR23369.1|
bicyclogermacrene synthase [Phyla dulcis]
Length = 565
Score = 253 bits (645), Expect(2) = 7e-91
Identities = 117/220 (53%), Positives = 164/220 (74%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L + P+YMK+IYK M +QG SY Y IEAIK + R+Y +E +WRE+KY
Sbjct: 344 LDQLPDYMKLIYKTVLEVYDEIEEEMIKQGTSYRTAYGIEAIKSLTRNYFMEAEWREKKY 403
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDI 1503
PT++E+MR+ +C Y SLI+ISF+GMG++ +EA +WVLS+PD VK++L I+RL +DI
Sbjct: 404 TPTTDEHMRLALKTCGYTSLIIISFLGMGEVVKREAFDWVLSEPDFVKASLTINRLVDDI 463
Query: 1504 AGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLL 1683
GHE E++R+H+ S VECY++E ++E+AV E ++E WKD+NEGFLKPT+FP+PLL
Sbjct: 464 VGHEDEQKRNHVVSSVECYVQESKTSREDAVYELNSRVESTWKDLNEGFLKPTKFPSPLL 523
Query: 1684 YCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
Y +LN++R+IE +Y K DWYT+VGPEMQ ++R LLIDPV
Sbjct: 524 YRVLNYSRVIEVMYTKG-DWYTNVGPEMQDYIRQLLIDPV 562
Score = 110 bits (276), Expect(2) = 7e-91
Identities = 46/75 (61%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+SRWWKG ++ L +ARDR+VETY+W+L V+FEP+Y +R I KVQ+L SI+DD FDA
Sbjct: 263 LSRWWKGIDVARNLPYARDRIVETYFWILGVYFEPEYAAARKILVKVQSLFSIIDDTFDA 322
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL +FTQA+E
Sbjct: 323 YGTFEELQIFTQALE 337
Score = 150 bits (378), Expect(2) = 1e-40
Identities = 77/156 (49%), Positives = 107/156 (68%), Gaps = 3/156 (1%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVE---EAKGLLEFFEAT 360
YC +NHD+Y +L F LLRQ GYRVSCEIF+KF+D + G++ + E G+LEF+EAT
Sbjct: 113 YCKDNHDLYANSLSFRLLRQQGYRVSCEIFEKFKDVK--GNFMLPNNGEVMGVLEFYEAT 170
Query: 361 YMRVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKY 540
++RV GE++L+ ++ L +LPSLT+ PLA++++HALH HS RRGL+RLEAR Y
Sbjct: 171 HLRVHGEDLLDHDFVLSREYLESVLPSLTN---PLAEQVDHALHQHSNRRGLSRLEARHY 227
Query: 541 IAVYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+ VYEQY SH + QS+H ++LSEL
Sbjct: 228 MPVYEQYASHDQYLLKLAKLDFNMLQSLHKEELSEL 263
Score = 45.8 bits (107), Expect(2) = 1e-40
Identities = 22/41 (53%), Positives = 31/41 (75%)
Frame = +3
Query: 63 ASNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
ASND+ + ++++DAIQRLG YHFEEEI++AL L + H
Sbjct: 72 ASNDY-MRLIQMVDAIQRLGFAYHFEEEIDQALQCLFERHH 111
>sp|J7LP58.1|TPS1_PHYDL RecName: Full=Bifunctional sesquiterpene synthase 1; AltName:
Full=Alpha-copaene synthase; AltName: Full=Delta-cadinene
synthase; AltName: Full=Terpene synthase 1; Short=LdTPS1
gi|403084368|gb|AFR23368.1| alpha-copaene/delta-cadinene
synthase [Phyla dulcis]
Length = 564
Score = 253 bits (646), Expect(2) = 5e-89
Identities = 119/220 (54%), Positives = 153/220 (69%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L + PEYMK++YK M + G + Y IEAIK R Y +E KWREEKY
Sbjct: 344 LDQLPEYMKLLYKTMLEVSDEIEEEMTKLGTPFRIAYGIEAIKTFARSYFLEAKWREEKY 403
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDI 1503
KPT+EEYM + T +C Y SLI+ SF+ MGD+ +E +WVLS PD V ++ +I RL++DI
Sbjct: 404 KPTTEEYMGLATKTCGYKSLIITSFLAMGDIPKREHFDWVLSDPDFVMASCIICRLADDI 463
Query: 1504 AGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLL 1683
GHE E+ RDHIPS VECY +EH +KE+AV E ++E WKD+NEGFL+PT+ PA LL
Sbjct: 464 VGHEFEQTRDHIPSSVECYTQEHKTSKEDAVNELYDRLESAWKDLNEGFLRPTKIPAALL 523
Query: 1684 YCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
Y +LN+ R+IE +Y + DWYTHVGPEMQ FVR LL+DPV
Sbjct: 524 YRVLNYCRIIEVMYSRG-DWYTHVGPEMQGFVRQLLVDPV 562
Score = 104 bits (259), Expect(2) = 5e-89
Identities = 43/75 (57%), Positives = 59/75 (78%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+SRWWKG ++ +ARDR+VE Y+WVL V+FEP+Y V R I+TKV A+ S++DD FDA
Sbjct: 263 ISRWWKGVDIARNFPYARDRIVELYFWVLGVYFEPQYAVGRKITTKVIAIASLLDDTFDA 322
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL +F +A+E
Sbjct: 323 YGTFEELRIFAEAVE 337
Score = 139 bits (351), Expect(2) = 3e-38
Identities = 69/154 (44%), Positives = 104/154 (67%), Gaps = 1/154 (0%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDW-RVEEAKGLLEFFEATYM 366
Y +++ D+Y +L F LLRQHGYR+SCEIF+KF+D G +E G+LEF+EAT++
Sbjct: 113 YSEDSQDMYANSLSFRLLRQHGYRISCEIFEKFKDANGGFKIPNIEGVMGMLEFYEATHL 172
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
RVRGE++L+ ++++ L +LPSL++ PLA +++ AL+ +S RRGL RLEAR +++
Sbjct: 173 RVRGEDILDHGFVFSRNYLKSVLPSLSN---PLAAQVDRALNQNSNRRGLPRLEARHFMS 229
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
VYEQY SH + QS+H +LSE+
Sbjct: 230 VYEQYASHDQALLKLAKLNFNILQSLHKVELSEI 263
Score = 48.5 bits (114), Expect(2) = 3e-38
Identities = 24/41 (58%), Positives = 31/41 (75%)
Frame = +3
Query: 63 ASNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
ASND+I L+ +D IQRLG+ YHFEEEI++AL L + FH
Sbjct: 72 ASNDYIR-LLQTVDVIQRLGLAYHFEEEIDQALRYLFETFH 111
>sp|Q49SP3.1|TPSPS_POGCB RecName: Full=Patchoulol synthase; Short=PatTps177; AltName:
Full=Alpha-guaiene synthase; AltName: Full=Delta-guaiene
synthase gi|46254783|gb|AAS86323.1| patchoulol synthase
[Pogostemon cablin] gi|86264162|gb|ABC87816.1| patchoulol
synthase [Pogostemon cablin]
Length = 552
Score = 232 bits (592), Expect(2) = 5e-80
Identities = 111/221 (50%), Positives = 152/221 (68%), Gaps = 1/221 (0%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L K P+YMKI+YK + + G Y A Y EA+K R Y+ E +WRE+K+
Sbjct: 331 LDKLPDYMKIVYKALLDVFEEVDEELIKLGAPYRAYYGKEAMKYAARAYMEEAQWREQKH 390
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGMGD-LATKEALEWVLSQPDMVKSTLLISRLSND 1500
KPT++EYM++ T +C YI+LI++S +G+ + + TKEA +WV S+P +++TL+I+RL ND
Sbjct: 391 KPTTKEYMKLATKTCGYITLIILSCLGVEEGIVTKEAFDWVFSRPPFIEATLIIARLVND 450
Query: 1501 IAGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPL 1680
I GHE E++R+H+ + VECYMEEH V K+ V EF Q+E WKDINEGFL+P EFP PL
Sbjct: 451 ITGHEFEKKREHVRTAVECYMEEHKVGKQEVVSEFYNQMESAWKDINEGFLRPVEFPIPL 510
Query: 1681 LYCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
LY ILN R +E IY K+ D YTHVGP MQ ++ L + PV
Sbjct: 511 LYLILNSVRTLEVIY-KEGDSYTHVGPAMQNIIKQLYLHPV 550
Score = 95.1 bits (235), Expect(2) = 5e-80
Identities = 42/74 (56%), Positives = 55/74 (74%)
Frame = +2
Query: 914 SRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDAY 1093
SRWWK + TKL+F RDRLVE+Y+W +FEP Y V+R I K A++S++DD +DAY
Sbjct: 251 SRWWKTLQVPTKLSFVRDRLVESYFWASGSYFEPNYSVARMILAKGLAVLSLMDDVYDAY 310
Query: 1094 GTFQELSLFTQAIE 1135
GTF+EL +FT AIE
Sbjct: 311 GTFEELQMFTDAIE 324
Score = 147 bits (372), Expect(2) = 6e-40
Identities = 74/153 (48%), Positives = 107/153 (69%), Gaps = 1/153 (0%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVEE-AKGLLEFFEATYM 366
+C NHD++ TAL F LLRQHGYRVSCE+F+KF+DG++G E+ A +LEFFEAT++
Sbjct: 100 FCKNNHDMHATALSFRLLRQHGYRVSCEVFEKFKDGKDGFKVPNEDGAVAVLEFFEATHL 159
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
RV GE+VL+ A +T++ L + +L D P A+++++AL+ S RRGL R+EARKYI+
Sbjct: 160 RVHGEDVLDNAFDFTRNYLESVYATLND---PTAKQVHNALNEFSFRRGLPRVEARKYIS 216
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSE 645
+YEQY SHH+ Q++H ++LSE
Sbjct: 217 IYEQYASHHKGLLKLAKLDFNLVQALHRRELSE 249
Score = 45.8 bits (107), Expect(2) = 6e-40
Identities = 20/39 (51%), Positives = 31/39 (79%)
Frame = +3
Query: 66 SNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
++D + QLK++DAIQRLG++Y F E+++EAL NL + F
Sbjct: 59 ASDNYMRQLKMVDAIQRLGIDYLFVEDVDEALKNLFEMF 97
>sp|Q49SP6.1|TPGD2_POGCB RecName: Full=Germacrene D synthase 2; AltName: Full=PatTpsBF2
gi|52139809|gb|AAS86320.1| germacrene D synthase
[Pogostemon cablin]
Length = 554
Score = 229 bits (584), Expect(2) = 2e-77
Identities = 112/221 (50%), Positives = 155/221 (70%), Gaps = 1/221 (0%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L + P+YMKIIYK M +QG Y +YA EA+K+++ Y VE KWREEK
Sbjct: 334 LDQLPDYMKIIYKALLGVFEVIEKLMIKQGTLYRLNYAKEAMKIVVGGYFVEAKWREEKS 393
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGM-GDLATKEALEWVLSQPDMVKSTLLISRLSND 1500
KPT++EYM+V T S Y++LI+ SF+GM ++ATKEA +WVLS+PD++K+ + ++RL+ND
Sbjct: 394 KPTTQEYMQVATKSAGYMTLIITSFLGMEANIATKEAFDWVLSEPDVMKAAITLARLTND 453
Query: 1501 IAGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPL 1680
I G ELE+ER HI + VE Y +EH ++ + A+ E K QIE GWK INE FL+PT+FP P+
Sbjct: 454 IVGIELEKERKHIATAVEVYEDEHKLSMQEAMVEIKNQIESGWKTINEAFLRPTKFPTPI 513
Query: 1681 LYCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
LY ILN+ R++E IY K D YTHV P +Q ++ L I P+
Sbjct: 514 LYRILNYCRVLEVIYDK-SDRYTHVDPALQDIIKQLYIHPI 553
Score = 89.4 bits (220), Expect(2) = 2e-77
Identities = 42/74 (56%), Positives = 53/74 (71%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+SRW++ + T L FAR RLVETY+W V FEP+ V+R I KVQ L+S +DD FDA
Sbjct: 253 LSRWYRDLEVPTMLPFARQRLVETYFWDAGVVFEPENDVARMILVKVQCLISFLDDTFDA 312
Query: 1091 YGTFQELSLFTQAI 1132
YG+F+EL LFT AI
Sbjct: 313 YGSFEELQLFTDAI 326
Score = 143 bits (361), Expect(2) = 1e-40
Identities = 71/160 (44%), Positives = 107/160 (66%), Gaps = 3/160 (1%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRV---EEAKGLLEFFEAT 360
YC + HD+Y AL F LLRQHGYR+SC++F K +DGE+G ++V +EA ++E EAT
Sbjct: 103 YCKDKHDLYAIALSFRLLRQHGYRISCDVFDKLKDGEDG--FKVPPSDEALAVVELLEAT 160
Query: 361 YMRVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKY 540
++R+ GE VL+RA + + L + +L N P+A+++++AL+G+S RRG+ ++EARKY
Sbjct: 161 HLRIHGEVVLDRAFVFARIHLESIEANL---NNPVAKQVHNALYGYSNRRGMQQVEARKY 217
Query: 541 IAVYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSELYSKY 660
I +YEQY SHH+ Q+MH ++LSEL Y
Sbjct: 218 IPIYEQYASHHQGLLKLATLNFNLQQTMHKRELSELSRWY 257
Score = 52.8 bits (125), Expect(2) = 1e-40
Identities = 26/40 (65%), Positives = 34/40 (85%)
Frame = +3
Query: 63 ASNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
ASND+ + QLK++DAIQRLG+E FEE+I+ AL NLS+NF
Sbjct: 62 ASNDY-MRQLKMVDAIQRLGIESLFEEDIDNALKNLSENF 100
>sp|Q49SP4.1|TPGD1_POGCB RecName: Full=Germacrene D synthase 1; AltName: Full=PatTpsB15
gi|46254781|gb|AAS86322.1| germacrene D synthase
[Pogostemon cablin]
Length = 545
Score = 215 bits (548), Expect(2) = 2e-76
Identities = 108/221 (48%), Positives = 148/221 (66%), Gaps = 1/221 (0%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L + PEYM+IIYK M +QG SY DYA EAIK++I Y E KWREE+Y
Sbjct: 325 LDELPEYMQIIYKLVLDVFEEIESHMIKQGTSYRLDYAREAIKIVIGGYFDEAKWREEEY 384
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGM-GDLATKEALEWVLSQPDMVKSTLLISRLSND 1500
KP EEYM+V T S AY++LI++SF+GM D+AT +A +WVLS+P ++ ++L ++RLSND
Sbjct: 385 KPRMEEYMKVATKSAAYLTLIIVSFVGMKNDIATPQAFQWVLSEPQIITASLALARLSND 444
Query: 1501 IAGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPL 1680
+ G E E+ER +I + VE Y EEH V+KE AV E + + E WK+INE L+PT F P+
Sbjct: 445 LVGIEFEKERKYIATAVELYEEEHKVSKEEAVLELRHETESAWKEINEALLEPTTFATPI 504
Query: 1681 LYCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
L ILN AR++E +++ D YTHV E+Q + L I P+
Sbjct: 505 LDRILNSARVLE-VFYDKTDRYTHVDLELQNIIAQLYIHPI 544
Score = 99.8 bits (247), Expect(2) = 2e-76
Identities = 44/73 (60%), Positives = 54/73 (73%)
Frame = +2
Query: 917 RWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDAYG 1096
RWWK + TKL + RDRLVETY+W + +FEP+Y ++RNI KVQ LVSI DD FDAYG
Sbjct: 246 RWWKDLEMPTKLPYIRDRLVETYFWDMGFYFEPQYALARNILVKVQCLVSIFDDTFDAYG 305
Query: 1097 TFQELSLFTQAIE 1135
F+EL LF AI+
Sbjct: 306 AFKELQLFKDAID 318
Score = 162 bits (410), Expect(2) = 8e-47
Identities = 75/154 (48%), Positives = 113/154 (73%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVEEAKGLLEFFEATYMR 369
YC +NHD+Y TAL F LLRQHGY+VSCE+F KF+DGE+G ++VEE +LE FEAT+MR
Sbjct: 97 YCKDNHDMYATALSFRLLRQHGYKVSCEVFDKFKDGEDG--FKVEEVMAVLELFEATHMR 154
Query: 370 VRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIAV 549
+ GE+VL++A +T++ L + +L++ P+A+++++AL+G+S RRG+ R+EARKYI +
Sbjct: 155 IHGEDVLDQAFVFTRNYLQSIHATLSN---PIAKQVHNALNGYSCRRGMPRIEARKYIPI 211
Query: 550 YEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSELY 651
YE+Y HH+ QSMH ++L++LY
Sbjct: 212 YEEYGCHHKALLKLAKLDFNLLQSMHKRELTQLY 245
Score = 54.3 bits (129), Expect(2) = 8e-47
Identities = 28/40 (70%), Positives = 33/40 (82%)
Frame = +3
Query: 63 ASNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
ASND+ L QLK++DAIQRLG+EY FEEEI+EAL NL F
Sbjct: 56 ASNDY-LRQLKMVDAIQRLGIEYLFEEEIDEALRNLLAKF 94
>gb|AHL24448.1| patchoulol synthase variant [Pogostemon cablin]
Length = 552
Score = 221 bits (562), Expect(2) = 5e-76
Identities = 109/221 (49%), Positives = 147/221 (66%), Gaps = 1/221 (0%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L K PEYMKI+YK + + G Y Y EA+K R Y+ E +WRE+K+
Sbjct: 331 LDKLPEYMKIVYKALLDVFEEVDEEVIKLGAPYRVYYGKEAMKYAARAYMEEAQWREQKH 390
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGMGD-LATKEALEWVLSQPDMVKSTLLISRLSND 1500
KPT++EYM++ T +C YI+LI++SF+G+ + + TKEA +WV S+P V++TL+I+RL ND
Sbjct: 391 KPTTKEYMKLATKTCGYITLIILSFLGVEEGIVTKEAFDWVFSRPPFVEATLIIARLIND 450
Query: 1501 IAGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPL 1680
I G E E +R+H+ + VECYMEEH V K+ V EF Q+E WKDINE L+P EFP PL
Sbjct: 451 ITGCEFENKREHVRTAVECYMEEHKVGKQEVVSEFYNQMESAWKDINECLLRPAEFPIPL 510
Query: 1681 LYCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
L ILN R +E IY K+ D YTHVGP MQ ++ L + PV
Sbjct: 511 LNLILNSVRTLEVIY-KEGDSYTHVGPAMQNIIKQLYLHPV 550
Score = 93.2 bits (230), Expect(2) = 5e-76
Identities = 41/74 (55%), Positives = 55/74 (74%)
Frame = +2
Query: 914 SRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDAY 1093
SRWWK + T+L+F RDRLVE+Y+W +FEP Y V+R I K A++S++DD +DAY
Sbjct: 251 SRWWKTLQVPTELSFVRDRLVESYFWASGSYFEPNYSVARMILAKGLAVLSLMDDVYDAY 310
Query: 1094 GTFQELSLFTQAIE 1135
GTF+EL +FT AIE
Sbjct: 311 GTFEELQVFTDAIE 324
Score = 148 bits (373), Expect(2) = 5e-40
Identities = 74/153 (48%), Positives = 106/153 (69%), Gaps = 1/153 (0%)
Frame = +1
Query: 190 YCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVEE-AKGLLEFFEATYM 366
+C NHD++ TAL F LLRQHGYRVSCE+F+KF+DG++G E+ A +LEFFEAT++
Sbjct: 100 FCKNNHDMHATALSFRLLRQHGYRVSCEVFEKFKDGKDGFKVPNEDGAVAVLEFFEATHL 159
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
RV GE+VL+ A +T++ L + +L D P A ++++AL+ S RRGL R+EARKYI+
Sbjct: 160 RVHGEDVLDNAFVFTRNYLESVYATLND---PTANQVHNALNEFSFRRGLPRVEARKYIS 216
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSE 645
+YEQY SHH+ Q++H ++LSE
Sbjct: 217 IYEQYASHHKGLLKLAKLDFNLVQALHRRELSE 249
Score = 45.8 bits (107), Expect(2) = 5e-40
Identities = 20/39 (51%), Positives = 31/39 (79%)
Frame = +3
Query: 66 SNDFILPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
++D + QLK++DAIQRLG++Y F E+++EAL NL + F
Sbjct: 59 ASDNYMRQLKMVDAIQRLGIDYLFVEDVDEALKNLFEMF 97
>ref|XP_002282488.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
gi|302142994|emb|CBI20289.3| unnamed protein product
[Vitis vinifera] gi|313755468|gb|ADR74225.1|
selina-411-diene/intermedeol synthase [synthetic
construct]
Length = 561
Score = 206 bits (525), Expect(2) = 2e-75
Identities = 101/220 (45%), Positives = 142/220 (64%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ Y+ M +QGK Y YA A+K ++ YL+E KW +++Y P
Sbjct: 343 QLPDYMKVCYQALLDVYEEMEEEMTKQGKLYRVHYAQAALKRQVQAYLLEAKWLKQEYIP 402
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
+EYM S A L SF+GMGD+ TKEA +WV S P M++++ +I RL +DI
Sbjct: 403 RMDEYMSNALVSSACSMLTTTSFVGMGDIVTKEAFDWVFSDPKMIRASNVICRLMDDIVS 462
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R H+ S VECYM+++ V+KE A EFKKQ+E WKD NE FL+PT P PLL
Sbjct: 463 HEFEQKRGHVASAVECYMKQYGVSKEEAYDEFKKQVESAWKDNNEEFLQPTAVPVPLLTR 522
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LNF+R+++ +Y KD+D YT VGP M+ V +LIDPV +
Sbjct: 523 VLNFSRMMDVLY-KDEDEYTLVGPLMKDLVAGMLIDPVPM 561
Score = 105 bits (262), Expect(2) = 2e-75
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWKG +TKL FARDR+VE Y+W+L V+FEP+Y ++R I KV ++SIVDD +DA
Sbjct: 260 ITKWWKGLDFATKLPFARDRIVEGYFWILGVYFEPQYYLARRILMKVFGVLSIVDDIYDA 319
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL LFT+AIE
Sbjct: 320 YGTFEELKLFTEAIE 334
Score = 94.7 bits (234), Expect(2) = 7e-24
Identities = 54/154 (35%), Positives = 88/154 (57%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWR---VEEAKGLLEFFEATYM 366
D+N + +L F LLRQ GY + ++FKKF D EGG+++ V + G+L +EA ++
Sbjct: 113 DDNDHLTTVSLLFRLLRQEGYHIPSDVFKKFMD--EGGNFKESLVGDLPGMLALYEAAHL 170
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
V GE++L+ A +T + L + D++ PL +++ AL R+GL R+EAR YI
Sbjct: 171 MVHGEDILDEALGFTTAHLQSMA---IDSDNPLTKQVIRALK-RPIRKGLPRVEARHYIT 226
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+Y++ SH+ QS+H K+LSE+
Sbjct: 227 IYQEDDSHNESLLKLAKLDYNMLQSLHRKELSEI 260
Score = 45.1 bits (105), Expect(2) = 7e-24
Identities = 21/31 (67%), Positives = 25/31 (80%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
LKLID+IQRLG+ YHFE EIEEAL + Q +
Sbjct: 79 LKLIDSIQRLGLAYHFEREIEEALKAMYQTY 109
>emb|CAN81129.1| hypothetical protein VITISV_002911 [Vitis vinifera]
Length = 561
Score = 204 bits (519), Expect(2) = 1e-74
Identities = 101/220 (45%), Positives = 139/220 (63%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ Y+ M +QGK Y YA A+K ++ YL+E KW +++Y P
Sbjct: 343 QLPDYMKVCYQALLDVYEEMEEEMXKQGKLYRVHYAQAALKXQVQAYLLEAKWLKQEYIP 402
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEYM A L SF+GMGD TKEA +WV S P M++++ +I RL +DI
Sbjct: 403 TMEEYMSNALVXSACSMLTTTSFVGMGDXVTKEAFDWVFSDPKMIRASNVICRLMDDIVS 462
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R H+ S VECYM+++ V+KE A EFKKQ+E WKD NE L+PT P PLL
Sbjct: 463 HEFEQKRGHVASAVECYMKQYGVSKEEAYDEFKKQVESAWKDNNEEXLQPTAVPVPLLTR 522
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LNF+R+ + +Y KD+D YT VGP M+ V +LIDPV +
Sbjct: 523 VLNFSRMXDVLY-KDEDEYTLVGPLMKDLVAGMLIDPVPM 561
Score = 105 bits (262), Expect(2) = 1e-74
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWKG +TKL FARDR+VE Y+W+L V+FEP+Y ++R I KV ++SIVDD +DA
Sbjct: 260 ITKWWKGLDFATKLPFARDRIVEGYFWILGVYFEPQYYLARRILMKVFGVLSIVDDIYDA 319
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL LFT+AIE
Sbjct: 320 YGTFEELKLFTEAIE 334
Score = 94.7 bits (234), Expect(2) = 7e-24
Identities = 54/154 (35%), Positives = 88/154 (57%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWR---VEEAKGLLEFFEATYM 366
D+N + +L F LLRQ GY + ++FKKF D EGG+++ V + G+L +EA ++
Sbjct: 113 DDNDHLTTVSLLFRLLRQEGYHIPSDVFKKFMD--EGGNFKESLVGDLPGMLALYEAAHL 170
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
V GE++L+ A +T + L + D++ PL +++ AL R+GL R+EAR YI
Sbjct: 171 MVHGEDILDEALGFTTAHLQSMA---IDSDNPLTKQVIRALK-RPIRKGLPRVEARHYIT 226
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+Y++ SH+ QS+H K+LSE+
Sbjct: 227 IYQEDDSHNESLLKLAKLDYNMLQSLHRKELSEI 260
Score = 45.1 bits (105), Expect(2) = 7e-24
Identities = 21/31 (67%), Positives = 25/31 (80%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNF 182
LKLID+IQRLG+ YHFE EIEEAL + Q +
Sbjct: 79 LKLIDSIQRLGLAYHFEREIEEALKAMYQTY 109
>emb|CBI20475.3| unnamed protein product [Vitis vinifera]
Length = 609
Score = 202 bits (513), Expect(2) = 2e-74
Identities = 98/220 (44%), Positives = 138/220 (62%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK Y+ M ++G Y YA AI+ I+ Y VE KW ++Y P
Sbjct: 391 QLPDYMKPCYQALLDVYEEMEEEMAKEGNLYRVQYAKAAIERQIQAYFVEAKWLNQEYTP 450
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T +EYM S Y L+ SF+GMGD+ATKEA +WV + P MV+++ +I RL +DI
Sbjct: 451 TLDEYMSNALISSGYSMLMTTSFVGMGDIATKEAFDWVFNDPKMVRASSVICRLMDDIVS 510
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R H+ S VECYM++H V K+ A E KQ+ WKD+N+ LKPT+ P PLL
Sbjct: 511 HEFEQKRGHVASAVECYMKQHGVCKQEAYDELNKQVVNAWKDMNKECLKPTQVPMPLLTR 570
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LNF+R+I+ I +KD+D YTHVG M+ + +LIDPV +
Sbjct: 571 VLNFSRVID-ILYKDEDQYTHVGKLMKDLIASILIDPVPI 609
Score = 107 bits (266), Expect(2) = 2e-74
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWKG + K+ FARDRLVE Y+W+L V+FEP+Y ++R I KV A++SI+DD +DA
Sbjct: 308 VTRWWKGLNFAKKMPFARDRLVEGYFWILGVYFEPQYSLARRILIKVLAMISIIDDIYDA 367
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL LFT+AIE
Sbjct: 368 YGTFEELKLFTEAIE 382
Score = 97.4 bits (241), Expect(2) = 1e-22
Identities = 57/154 (37%), Positives = 90/154 (58%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVE---EAKGLLEFFEATYM 366
D+N D+ +L F LLRQ GY + ++F KF+D E G+++ + GLL +EAT++
Sbjct: 161 DDNDDLTNASLRFRLLRQEGYHIPPDVFNKFKDDE--GNFKESLTGDLPGLLALYEATHL 218
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
V GE++LE A A++ + L + TD+ PLA ++ AL R+ LTR+EAR YI+
Sbjct: 219 MVHGEDILEEALAFSTAHLQSMA---TDSTHPLAAQVTRALK-RPIRKCLTRVEARHYIS 274
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
VY++ H++ QS+H K+LSE+
Sbjct: 275 VYQEDGPHNKTLLKLSKLDFNLLQSLHRKELSEV 308
Score = 38.1 bits (87), Expect(2) = 1e-22
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
L ID+IQRLG+ Y FE EI+EAL ++ Q ++
Sbjct: 126 LNFIDSIQRLGLAYQFEREIKEALEDMYQTYN 157
>ref|XP_002283076.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
Length = 559
Score = 202 bits (513), Expect(2) = 2e-74
Identities = 98/220 (44%), Positives = 138/220 (62%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK Y+ M ++G Y YA AI+ I+ Y VE KW ++Y P
Sbjct: 341 QLPDYMKPCYQALLDVYEEMEEEMAKEGNLYRVQYAKAAIERQIQAYFVEAKWLNQEYTP 400
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T +EYM S Y L+ SF+GMGD+ATKEA +WV + P MV+++ +I RL +DI
Sbjct: 401 TLDEYMSNALISSGYSMLMTTSFVGMGDIATKEAFDWVFNDPKMVRASSVICRLMDDIVS 460
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R H+ S VECYM++H V K+ A E KQ+ WKD+N+ LKPT+ P PLL
Sbjct: 461 HEFEQKRGHVASAVECYMKQHGVCKQEAYDELNKQVVNAWKDMNKECLKPTQVPMPLLTR 520
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LNF+R+I+ I +KD+D YTHVG M+ + +LIDPV +
Sbjct: 521 VLNFSRVID-ILYKDEDQYTHVGKLMKDLIASILIDPVPI 559
Score = 107 bits (266), Expect(2) = 2e-74
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWKG + K+ FARDRLVE Y+W+L V+FEP+Y ++R I KV A++SI+DD +DA
Sbjct: 258 VTRWWKGLNFAKKMPFARDRLVEGYFWILGVYFEPQYSLARRILIKVLAMISIIDDIYDA 317
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL LFT+AIE
Sbjct: 318 YGTFEELKLFTEAIE 332
Score = 97.4 bits (241), Expect(2) = 1e-22
Identities = 57/154 (37%), Positives = 90/154 (58%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVE---EAKGLLEFFEATYM 366
D+N D+ +L F LLRQ GY + ++F KF+D E G+++ + GLL +EAT++
Sbjct: 111 DDNDDLTNASLRFRLLRQEGYHIPPDVFNKFKDDE--GNFKESLTGDLPGLLALYEATHL 168
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
V GE++LE A A++ + L + TD+ PLA ++ AL R+ LTR+EAR YI+
Sbjct: 169 MVHGEDILEEALAFSTAHLQSMA---TDSTHPLAAQVTRALK-RPIRKCLTRVEARHYIS 224
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
VY++ H++ QS+H K+LSE+
Sbjct: 225 VYQEDGPHNKTLLKLSKLDFNLLQSLHRKELSEV 258
Score = 38.1 bits (87), Expect(2) = 1e-22
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
L ID+IQRLG+ Y FE EI+EAL ++ Q ++
Sbjct: 76 LNFIDSIQRLGLAYQFEREIKEALEDMYQTYN 107
>emb|CBI20288.3| unnamed protein product [Vitis vinifera]
Length = 682
Score = 207 bits (526), Expect(2) = 2e-74
Identities = 98/220 (44%), Positives = 142/220 (64%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ Y+ M +Q K Y YA +A+K ++ Y VE KW +++Y P
Sbjct: 289 QLPDYMKLCYQALLDVFEEIEEEMTKQEKPYRVHYAKQAMKKQVQAYFVEAKWLKQEYIP 348
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEYM SC Y L ISF+GMGD+ TK+A +WV S P MV+++ +I RL +DI
Sbjct: 349 TIEEYMTNALVSCGYFQLATISFVGMGDMVTKKAFDWVFSDPKMVRASAIICRLMDDIVD 408
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R ++ S V+CYM+++ VT++ A EF +I WKD+NE LK T+ P PLL C
Sbjct: 409 HEFEQKRGYVVSRVQCYMKQYGVTEQEACEEFNNEIMNAWKDMNEECLKRTQVPMPLLTC 468
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
++NFAR+I+ +Y K+QD YTHVG M+ + +LIDPV +
Sbjct: 469 VVNFARVIDLLY-KNQDEYTHVGKLMKDLIAWMLIDPVPM 507
Score = 101 bits (252), Expect(2) = 2e-74
Identities = 43/75 (57%), Positives = 59/75 (78%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWKG +TKL FARDR+VE Y+WVL V+FEP+Y ++R I KV A+ SI+DD +D
Sbjct: 206 ITKWWKGLDFATKLPFARDRIVEGYFWVLGVYFEPQYFLARRILLKVFAMTSIIDDIYDT 265
Query: 1091 YGTFQELSLFTQAIE 1135
+GTF+EL LF +AIE
Sbjct: 266 HGTFEELKLFAEAIE 280
Score = 180 bits (456), Expect = 2e-42
Identities = 90/192 (46%), Positives = 127/192 (66%)
Frame = +1
Query: 1234 KSYCADYAIEAIKVMIRDYLVEVKWREEKYKPTSEEYMRVGTASCAYISLILISFIGMGD 1413
K A I+ + + ++ YL+E KW +++Y P EEYM S A L SF+GMGD
Sbjct: 494 KDLIAWMLIDPVPMQVQAYLLEAKWLKQEYIPRMEEYMSNALVSSACSMLTTTSFVGMGD 553
Query: 1414 LATKEALEWVLSQPDMVKSTLLISRLSNDIAGHELERERDHIPSIVECYMEEHNVTKENA 1593
+ TKEA +WV S P M++++ +I RL +DI HE ++R H+ S VECYM+++ V+KE A
Sbjct: 554 IVTKEAFDWVFSDPKMIRASNVICRLMDDIVSHE--QKRGHVASAVECYMKQYGVSKEEA 611
Query: 1594 VREFKKQIEEGWKDINEGFLKPTEFPAPLLYCILNFARLIENIYFKDQDWYTHVGPEMQT 1773
EFKKQ+E WKD NE FL+PT P PLL +LNF+R+++ +Y KD+D YT VGP M+
Sbjct: 612 YDEFKKQVESAWKDNNEEFLQPTAVPVPLLTRVLNFSRMMDVLY-KDEDEYTLVGPLMKD 670
Query: 1774 FVRLLLIDPVAL 1809
V +LIDPV +
Sbjct: 671 LVAGMLIDPVPM 682
>ref|XP_003634645.1| PREDICTED: (-)-germacrene D synthase-like [Vitis vinifera]
Length = 672
Score = 206 bits (524), Expect(2) = 4e-74
Identities = 98/220 (44%), Positives = 143/220 (65%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ Y+ M +Q K Y YA +A+K +++ Y VE KW +++Y P
Sbjct: 454 QLPDYMKLCYQALLDVFEEIEEEMTKQEKPYRVHYAKQAMKKLVQAYFVEAKWLKQEYIP 513
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEYM SC Y L ISF+GMGD+ TK+A +WV S P MV+++ +I RL +DI
Sbjct: 514 TIEEYMTNALVSCGYFQLATISFVGMGDMVTKKAFDWVFSDPKMVRASAIICRLMDDIVD 573
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R ++ S V+CYM+++ VT++ A EF +I WKD+NE LK T+ P PLL C
Sbjct: 574 HEFEQKRGYVVSGVQCYMKQYGVTEQEACEEFINEIMNAWKDMNEECLKRTQVPMPLLTC 633
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
++NFAR+I+ +Y K+QD YTHVG M+ + +LIDPV +
Sbjct: 634 VVNFARVIDLLY-KNQDEYTHVGKLMKDLIAWMLIDPVPM 672
Score = 101 bits (252), Expect(2) = 4e-74
Identities = 43/75 (57%), Positives = 59/75 (78%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWKG +TKL FARDR+VE Y+WVL V+FEP+Y ++R I KV A+ SI+DD +D
Sbjct: 371 ITKWWKGLDFATKLPFARDRIVEGYFWVLGVYFEPQYFLARRILLKVFAMTSIIDDIYDT 430
Query: 1091 YGTFQELSLFTQAIE 1135
+GTF+EL LF +AIE
Sbjct: 431 HGTFEELKLFAEAIE 445
Score = 112 bits (279), Expect = 8e-22
Identities = 62/172 (36%), Positives = 101/172 (58%), Gaps = 2/172 (1%)
Frame = +1
Query: 139 KRRLKKLCLISLKTSIFYCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGG--D 312
+R ++ + +T D+N D+ AL F LLRQ GY + ++FKKF+D EEG +
Sbjct: 205 EREIEDALKVMYETYNLVDDDNDDLASAALRFRLLRQEGYHIPSDVFKKFKD-EEGNFKE 263
Query: 313 WRVEEAKGLLEFFEATYMRVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALH 492
V + G+L +EAT++ V GE++L+ A A+T + L + P D++ PL +++ HAL
Sbjct: 264 SLVGDLPGMLALYEATHLMVHGEDILDEAQAFTTAHLQSMAP---DSDNPLKKQVIHALE 320
Query: 493 GHSKRRGLTRLEARKYIAVYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
R+ L R+EAR YI++Y++Y SH++ QS+H K+LSE+
Sbjct: 321 -RPIRKSLIRVEARHYISIYQEYDSHNKSLLKLAKLDFNLLQSLHRKELSEI 371
Score = 79.0 bits (193), Expect = 8e-12
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = +2
Query: 917 RWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFD 1087
RWWK +TKL FARDRLVE Y+WVL V+FEP+Y+++R + TKV A SIVDD ++
Sbjct: 27 RWWKDLDFATKLPFARDRLVECYFWVLGVWFEPQYLLARRVLTKVMATTSIVDDIWN 83
>emb|CBI20287.3| unnamed protein product [Vitis vinifera]
Length = 595
Score = 206 bits (524), Expect(2) = 4e-74
Identities = 98/220 (44%), Positives = 143/220 (65%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ Y+ M +Q K Y YA +A+K +++ Y VE KW +++Y P
Sbjct: 377 QLPDYMKLCYQALLDVFEEIEEEMTKQEKPYRVHYAKQAMKKLVQAYFVEAKWLKQEYIP 436
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEYM SC Y L ISF+GMGD+ TK+A +WV S P MV+++ +I RL +DI
Sbjct: 437 TIEEYMTNALVSCGYFQLATISFVGMGDMVTKKAFDWVFSDPKMVRASAIICRLMDDIVD 496
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R ++ S V+CYM+++ VT++ A EF +I WKD+NE LK T+ P PLL C
Sbjct: 497 HEFEQKRGYVVSGVQCYMKQYGVTEQEACEEFINEIMNAWKDMNEECLKRTQVPMPLLTC 556
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
++NFAR+I+ +Y K+QD YTHVG M+ + +LIDPV +
Sbjct: 557 VVNFARVIDLLY-KNQDEYTHVGKLMKDLIAWMLIDPVPM 595
Score = 101 bits (252), Expect(2) = 4e-74
Identities = 43/75 (57%), Positives = 59/75 (78%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWKG +TKL FARDR+VE Y+WVL V+FEP+Y ++R I KV A+ SI+DD +D
Sbjct: 294 ITKWWKGLDFATKLPFARDRIVEGYFWVLGVYFEPQYFLARRILLKVFAMTSIIDDIYDT 353
Query: 1091 YGTFQELSLFTQAIE 1135
+GTF+EL LF +AIE
Sbjct: 354 HGTFEELKLFAEAIE 368
Score = 112 bits (279), Expect = 8e-22
Identities = 62/172 (36%), Positives = 101/172 (58%), Gaps = 2/172 (1%)
Frame = +1
Query: 139 KRRLKKLCLISLKTSIFYCDENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGG--D 312
+R ++ + +T D+N D+ AL F LLRQ GY + ++FKKF+D EEG +
Sbjct: 128 EREIEDALKVMYETYNLVDDDNDDLASAALRFRLLRQEGYHIPSDVFKKFKD-EEGNFKE 186
Query: 313 WRVEEAKGLLEFFEATYMRVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALH 492
V + G+L +EAT++ V GE++L+ A A+T + L + P D++ PL +++ HAL
Sbjct: 187 SLVGDLPGMLALYEATHLMVHGEDILDEAQAFTTAHLQSMAP---DSDNPLKKQVIHALE 243
Query: 493 GHSKRRGLTRLEARKYIAVYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
R+ L R+EAR YI++Y++Y SH++ QS+H K+LSE+
Sbjct: 244 -RPIRKSLIRVEARHYISIYQEYDSHNKSLLKLAKLDFNLLQSLHRKELSEI 294
>ref|XP_007021122.1| Delta-cadinene synthase isozyme A, putative [Theobroma cacao]
gi|508720750|gb|EOY12647.1| Delta-cadinene synthase
isozyme A, putative [Theobroma cacao]
Length = 716
Score = 209 bits (531), Expect(2) = 6e-74
Identities = 95/220 (43%), Positives = 140/220 (63%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK+ YK M +QGK + ++ E +K +++ Y E KW E Y P
Sbjct: 497 QLPDYMKLCYKALLDVYEEMEEVMTKQGKLFRVQHSKEEMKRLVQAYFAEAKWLHENYTP 556
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEYM + SC Y L++ S +GMGD+ KEA W + P +V+++ +I+RL ND +
Sbjct: 557 TMEEYMPIALKSCGYYMLLMTSLVGMGDIIPKEAFHWASNDPKIVRASSIINRLMNDTSA 616
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
H+ E+ER H+PS VECYM+++ V +E A EF+KQ+E+ WKDINE FLKPT P P+L
Sbjct: 617 HKFEQERRHVPSAVECYMKQYGVFEEEAYDEFRKQVEDAWKDINEQFLKPTAVPEPVLIR 676
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
LN +R+I+ +Y D YTHVG E+++ + LLIDP+ +
Sbjct: 677 ALNLSRVIQLLYKDGSDTYTHVGEELKSRITSLLIDPLPI 716
Score = 98.2 bits (243), Expect(2) = 6e-74
Identities = 43/72 (59%), Positives = 54/72 (75%)
Frame = +2
Query: 917 RWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDAYG 1096
RWWK F S KL F RDR+VE Y+WVL V+FEP+Y ++R + KV A+ SI+DD +DAYG
Sbjct: 416 RWWKDFEFSDKLPFIRDRVVEDYFWVLGVYFEPQYSLARRMMAKVIAMTSIIDDIYDAYG 475
Query: 1097 TFQELSLFTQAI 1132
TF+EL LF AI
Sbjct: 476 TFEELELFASAI 487
Score = 102 bits (253), Expect(2) = 1e-25
Identities = 58/154 (37%), Positives = 91/154 (59%), Gaps = 3/154 (1%)
Frame = +1
Query: 199 ENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWR---VEEAKGLLEFFEATYMR 369
+ +D+Y T+L F LLR+HG+ + CE F KF+D E G+++ + + +GLLE +EA ++R
Sbjct: 268 DGNDLYITSLRFRLLREHGFNIQCESFNKFKD--EKGNFKASLISDVRGLLELYEAAHLR 325
Query: 370 VRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIAV 549
V GE +LE A A+T S L +L+ ++ + PL+ E+ AL R+ L RLEAR+YI
Sbjct: 326 VHGENILEEALAFTTSHL-RLVETVVE--YPLSAEVASALKS-PIRKNLPRLEARRYIPT 381
Query: 550 YEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSELY 651
Y+ H Q +H K++SE+Y
Sbjct: 382 YQACALHDETLLKFAKLDFNLLQYLHKKEISEIY 415
Score = 43.9 bits (102), Expect(2) = 1e-25
Identities = 20/28 (71%), Positives = 25/28 (89%)
Frame = +3
Query: 87 QLKLIDAIQRLGVEYHFEEEIEEALLNL 170
+L LIDA+QRLGV YHFE+EIE+AL N+
Sbjct: 234 KLLLIDAVQRLGVAYHFEKEIEDALDNI 261
>ref|XP_002283070.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
gi|147769765|emb|CAN76781.1| hypothetical protein
VITISV_000919 [Vitis vinifera]
gi|302143181|emb|CBI20476.3| unnamed protein product
[Vitis vinifera] gi|313755470|gb|ADR74226.1|
cubebol/delta-cadinene synthase [synthetic construct]
Length = 559
Score = 200 bits (508), Expect(2) = 6e-74
Identities = 99/220 (45%), Positives = 137/220 (62%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ P+YMK Y+ + ++G Y YA AI+ I+ Y VE KW ++Y P
Sbjct: 341 QLPDYMKPCYQALLDVYEEMEEEIAKEGNLYRVQYAKAAIQRQIQAYFVEAKWLNQEYTP 400
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T +EYM S Y LI SF+GMGD+ATKEA +WV S P MV+++ +I RL +DI
Sbjct: 401 TLDEYMSNALVSSGYSMLITTSFVGMGDIATKEAFDWVFSDPKMVRASSVICRLMDDIVS 460
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
HE E++R H+ S VECYM++ V K+ A E KQ+ GWKD+NE LKPT+ P PLL
Sbjct: 461 HEFEQKRGHVASAVECYMKQRGVCKQEAYDELNKQVVNGWKDMNEECLKPTQVPMPLLTR 520
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LNF+R+I+ I +KD+D YTHVG M+ + +LID V +
Sbjct: 521 VLNFSRVID-ILYKDEDQYTHVGKLMKDLIASILIDLVPI 559
Score = 107 bits (266), Expect(2) = 6e-74
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWKG + K+ FARDRLVE Y+W+L V+FEP+Y ++R I KV A++SI+DD +DA
Sbjct: 258 VTRWWKGLNFAKKMPFARDRLVEGYFWILGVYFEPQYSLARRILIKVLAMISIIDDIYDA 317
Query: 1091 YGTFQELSLFTQAIE 1135
YGTF+EL LFT+AIE
Sbjct: 318 YGTFEELKLFTEAIE 332
Score = 92.8 bits (229), Expect(2) = 3e-21
Identities = 55/154 (35%), Positives = 87/154 (56%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRVE---EAKGLLEFFEATYM 366
D+N D+ +L F LLRQ GY + ++F KF+D E G+++ + GLL +EAT++
Sbjct: 111 DDNDDLTNASLRFRLLRQEGYHIPPDVFNKFKDDE--GNFKESLTGDLPGLLALYEATHL 168
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
GE +LE A A++ + L + TD+ PL ++ AL R+ LTR+EAR YI+
Sbjct: 169 MAHGEAILEEALAFSTAHLQSMA---TDSTHPLPAQVTRALK-RPIRKCLTRVEARHYIS 224
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
VY++ H++ QS+H K+LSE+
Sbjct: 225 VYQEDGPHNKTLLKLSKLDFNLLQSLHRKELSEV 258
Score = 38.1 bits (87), Expect(2) = 3e-21
Identities = 17/32 (53%), Positives = 24/32 (75%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
L ID+IQRLG+ Y FE EI+EAL ++ Q ++
Sbjct: 76 LNFIDSIQRLGLAYQFEREIKEALEDMYQTYN 107
>ref|XP_007021115.1| Delta-cadinene synthase isozyme A, putative [Theobroma cacao]
gi|508720743|gb|EOY12640.1| Delta-cadinene synthase
isozyme A, putative [Theobroma cacao]
Length = 566
Score = 208 bits (530), Expect(2) = 2e-73
Identities = 103/218 (47%), Positives = 137/218 (62%)
Frame = +1
Query: 1156 PEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKPTS 1335
PEYM+I Y+ M +GK Y DYAIEA+K +R Y E +W + PT
Sbjct: 350 PEYMQICYQALLDVYKETEETMTEEGKLYRLDYAIEAMKNQVRAYFREAEWFHNHHVPTM 409
Query: 1336 EEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAGHE 1515
EEYM V + AY L SF+GMGD+ATKE EW+ S P MV ++ ++ RL +DI H+
Sbjct: 410 EEYMAVALVTSAYAMLATTSFVGMGDVATKEVFEWLFSDPKMVTASSIVCRLMDDIVSHK 469
Query: 1516 LERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYCIL 1695
E++R H+ S +ECY ++H T+E AV+EF+KQI + WKDINE L PT P PLL IL
Sbjct: 470 FEQKRGHVASAIECYTKQHGATEEEAVKEFRKQIADAWKDINEECLYPTTVPMPLLTRIL 529
Query: 1696 NFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
N AR+I+ +Y K +D YTH G ++ FV LLIDPV +
Sbjct: 530 NLARVIDVVY-KTEDGYTHAGVMLKDFVASLLIDPVPI 566
Score = 97.1 bits (240), Expect(2) = 2e-73
Identities = 39/75 (52%), Positives = 57/75 (76%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+++WWK S KL F RDR++E Y+W+L V+FEP+Y ++R I TKV A+ S++DD +D
Sbjct: 265 IAKWWKDLDFSRKLPFIRDRVIECYFWILGVYFEPEYRLARRILTKVIAMTSVIDDIYDV 324
Query: 1091 YGTFQELSLFTQAIE 1135
YGT +EL+LFT A+E
Sbjct: 325 YGTLEELALFTSAVE 339
Score = 102 bits (254), Expect(2) = 9e-25
Identities = 61/168 (36%), Positives = 96/168 (57%), Gaps = 4/168 (2%)
Frame = +1
Query: 157 LCLISLKTSIFYCD---ENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGE-EGGDWRVE 324
L L + +S CD + D+Y +L F LLRQ+GY++S + F KF D E + + +
Sbjct: 102 LVLQQMHSSHDDCDIGVNDEDLYTISLKFRLLRQNGYKMSSDAFNKFRDSEGKFKECLIN 161
Query: 325 EAKGLLEFFEATYMRVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSK 504
+ +G+L +EAT++RV GE++L++A A+T + L + L N LA +I+ AL
Sbjct: 162 DVQGMLSLYEATHLRVHGEDILDQALAFTTTHLETMATCL---NSSLAAQISQALR-QPI 217
Query: 505 RRGLTRLEARKYIAVYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
R+GL R+EAR YI VYE+ PSH Q +H +++SE+
Sbjct: 218 RKGLPRIEARHYIPVYEEDPSHSEAVLTFAKLDFNMLQRLHREEVSEI 265
Score = 40.4 bits (93), Expect(2) = 9e-25
Identities = 19/27 (70%), Positives = 22/27 (81%)
Frame = +3
Query: 81 LPQLKLIDAIQRLGVEYHFEEEIEEAL 161
L +LKLIDAIQRLG YHFE+EI+ L
Sbjct: 78 LEKLKLIDAIQRLGFSYHFEKEIDLVL 104
>gb|AAX16121.1| germacrene-D synthase [Actinidia deliciosa]
Length = 565
Score = 203 bits (517), Expect(2) = 2e-72
Identities = 98/222 (44%), Positives = 140/222 (63%)
Frame = +1
Query: 1144 LFKFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKY 1323
L + PEYMK+ Y+ M +QG+SYC DYA ++K+++R Y E KW + Y
Sbjct: 344 LDQLPEYMKLCYQALLDVYSMIDEEMAKQGRSYCVDYAKSSMKILVRAYFEEAKWFHQGY 403
Query: 1324 KPTSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDI 1503
PT EEYM+V + Y L SF+GMGDLATKEA +WV + P +V++ +I RL +DI
Sbjct: 404 VPTMEEYMQVALVTAGYKMLATSSFVGMGDLATKEAFDWVSNDPLIVQAASVIGRLKDDI 463
Query: 1504 AGHELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLL 1683
GH+ E++R H+ S VECY ++H T+E A+ E KQ+ WKDIN L P + P PLL
Sbjct: 464 VGHKFEQKRGHVASAVECYSKQHGTTEEEAIIELDKQVTHSWKDINAECLCPIKVPMPLL 523
Query: 1684 YCILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
+LN AR++ IY +D+D YTH G +++ FV +LID + +
Sbjct: 524 ARVLNLARVLYVIY-QDEDGYTHPGTKVENFVTSVLIDSMPI 564
Score = 99.0 bits (245), Expect(2) = 2e-72
Identities = 41/75 (54%), Positives = 58/75 (77%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWK + L FARDR+VE Y+W+L V+FEP+Y+++R + TKV A++SI+DD +D
Sbjct: 263 ITRWWKDLNFAKTLPFARDRMVECYFWILGVYFEPQYLLARRMLTKVIAMISIIDDIYDV 322
Query: 1091 YGTFQELSLFTQAIE 1135
YGT +EL LFT AIE
Sbjct: 323 YGTLEELVLFTDAIE 337
Score = 97.8 bits (242), Expect(2) = 1e-23
Identities = 55/154 (35%), Positives = 90/154 (58%), Gaps = 2/154 (1%)
Frame = +1
Query: 193 CDENHD-IYPTALGFHLLRQHGYRVSCEIFKKFEDGE-EGGDWRVEEAKGLLEFFEATYM 366
CD + D ++ AL F LLRQ GY VSC++F KF++ + E + + + +G+L +EAT +
Sbjct: 114 CDYHFDDLHTAALSFRLLRQQGYPVSCDMFDKFKNSKGEFQESIISDVQGMLSLYEATCL 173
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
R+ GE++L+ A A+T ++L LP+L + P ++I HAL+ +GLTRL AR +I
Sbjct: 174 RIHGEDILDEALAFTITQLRSALPNL---STPFKEQIIHALN-QPIHKGLTRLNARSHIL 229
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+EQ H + Q +H ++L E+
Sbjct: 230 FFEQNDCHSKDLLNFAKLDFNLLQKLHQRELCEI 263
Score = 41.2 bits (95), Expect(2) = 1e-23
Identities = 20/35 (57%), Positives = 26/35 (74%), Gaps = 2/35 (5%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQ--NFHF 188
L L+D IQRLGV YHFE EIE AL ++ + ++HF
Sbjct: 84 LNLVDKIQRLGVSYHFETEIETALRHIYKTCDYHF 118
>ref|XP_004288818.1| PREDICTED: (-)-germacrene D synthase-like [Fragaria vesca subsp.
vesca]
Length = 560
Score = 201 bits (511), Expect(2) = 5e-72
Identities = 96/218 (44%), Positives = 133/218 (61%)
Frame = +1
Query: 1156 PEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKPTS 1335
PEYMK Y+ + +GKSY DYA EA+K +R Y E KW Y PT
Sbjct: 344 PEYMKFCYEALLDVYAEAEQGLASEGKSYRIDYAKEAMKRQVRAYHAEAKWFHNNYTPTM 403
Query: 1336 EEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAGHE 1515
+EYM V + AY L SF+GMGD+ TK++ EW+ S P MVK++ ++ RL +DI H+
Sbjct: 404 DEYMEVALVTSAYSMLATTSFVGMGDIVTKDSFEWIFSDPKMVKASAVVCRLMDDIVSHK 463
Query: 1516 LERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYCIL 1695
E++R H+ S VECYM ++ T+E + EF+KQ+ + WKDINE L PT PLL +L
Sbjct: 464 FEQKRGHVASAVECYMIQYGATEEETIIEFRKQVSDAWKDINEECLHPTSVNMPLLMRVL 523
Query: 1696 NFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
N R+I+ +Y K +D YTH G ++ FV LL+DPV +
Sbjct: 524 NLTRVIDVLY-KSEDGYTHAGTILKDFVASLLVDPVTV 560
Score = 99.8 bits (247), Expect(2) = 5e-72
Identities = 42/75 (56%), Positives = 57/75 (76%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWK ++KL FARDR++E Y+W+L V+FEPKY +R TKV A+ SI+DD +D
Sbjct: 259 IARWWKKLDFASKLPFARDRVIECYFWILGVYFEPKYYFARRTLTKVIAMTSIIDDIYDV 318
Query: 1091 YGTFQELSLFTQAIE 1135
YGT +EL LFT+AIE
Sbjct: 319 YGTLEELDLFTRAIE 333
Score = 97.8 bits (242), Expect(2) = 1e-23
Identities = 56/152 (36%), Positives = 91/152 (59%), Gaps = 2/152 (1%)
Frame = +1
Query: 199 ENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWRV-EEAKGLLEFFEATYMRVR 375
++ D++ AL F LLRQHGY VSC++F KF+D + D + + GL +EAT++RVR
Sbjct: 112 DDDDLHTCALRFRLLRQHGYNVSCDMFNKFKDCDGKFDESLHHDVAGLQSLYEATHLRVR 171
Query: 376 GEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIAVYE 555
GE+ LE A A+T + L L+ PL++++ HAL+ R+GL RLEAR Y+++++
Sbjct: 172 GEDFLEEALAFTTTHLQSAANRLSP---PLSKQVRHALN-QPLRKGLPRLEARHYMSLHQ 227
Query: 556 Q-YPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+ + S + Q +H K+LS++
Sbjct: 228 ELHGSRNHVLLRFAKLDFNLLQQVHQKELSDI 259
Score = 41.2 bits (95), Expect(2) = 1e-23
Identities = 18/35 (51%), Positives = 26/35 (74%)
Frame = +3
Query: 81 LPQLKLIDAIQRLGVEYHFEEEIEEALLNLSQNFH 185
L +L+LID IQRLGV YHFE EI++ + + +N +
Sbjct: 74 LGKLELIDDIQRLGVSYHFEYEIDQTMQQIHENLN 108
>ref|XP_002283344.1| PREDICTED: (-)-germacrene D synthase [Vitis vinifera]
gi|302142880|emb|CBI20175.3| unnamed protein product
[Vitis vinifera] gi|313755474|gb|ADR74228.1|
(E)-beta-caryophyllene/2-epi-(E)-beta-caryophyllene
synthase [synthetic construct]
Length = 560
Score = 196 bits (499), Expect(2) = 1e-71
Identities = 95/217 (43%), Positives = 137/217 (63%)
Frame = +1
Query: 1159 EYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKPTSE 1338
+YMK Y+ M ++GK Y YA EA+K I+ Y +E KW ++Y PT +
Sbjct: 345 DYMKWFYRALLDVYEEMEEEMAKEGKLYRVHYAKEAMKRQIQAYFIEAKWLNQRYVPTFD 404
Query: 1339 EYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAGHEL 1518
EY+ SC Y LI SF+GMGD+ T+EA +WVLS M++++ ISRL +D+ HE
Sbjct: 405 EYISNTLVSCGYTLLIATSFVGMGDVVTEEAFQWVLSHAKMIRASETISRLMDDLVSHEF 464
Query: 1519 ERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYCILN 1698
E+ R H PS +ECYM+++ VTK+ A E KQI WKDIN+ L+PT+ P PL+ +LN
Sbjct: 465 EQTRMHAPSSIECYMKQYEVTKQEACDELNKQIVRAWKDINQECLRPTQVPMPLVTRVLN 524
Query: 1699 FARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPVAL 1809
F+R+I+ I +KD+D YTH G ++ + +LIDPV +
Sbjct: 525 FSRVID-ILYKDEDEYTHNGKLIKDLITWMLIDPVPM 560
Score = 102 bits (255), Expect(2) = 1e-71
Identities = 43/75 (57%), Positives = 60/75 (80%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
+SRWWKG ++TKL FARDRLVE+Y+W L V+FEP+Y +R TK+ A+++I+DD +DA
Sbjct: 258 ISRWWKGLDVATKLPFARDRLVESYFWTLGVYFEPQYFPARRFLTKMTAMLTIMDDIYDA 317
Query: 1091 YGTFQELSLFTQAIE 1135
YGT +EL L T+A+E
Sbjct: 318 YGTIEELELLTEAVE 332
Score = 105 bits (261), Expect(2) = 3e-25
Identities = 58/152 (38%), Positives = 91/152 (59%), Gaps = 1/152 (0%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEG-GDWRVEEAKGLLEFFEATYMRV 372
D+N D +L F LLRQ GY + ++F KF+D E ++ + ++ G+L +EAT++ V
Sbjct: 111 DDNDDFTNASLRFRLLRQEGYPIPSDVFNKFKDKEGNFKEYLIGDSLGMLALYEATHLMV 170
Query: 373 RGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIAVY 552
GE++LE A A+T + L + TD N PLA+++ AL R+GLTR+EA YI++Y
Sbjct: 171 HGEDILEEALAFTTTHLQSMA---TDPNNPLAKQVIRALK-RPIRKGLTRVEATHYISIY 226
Query: 553 EQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
+Q SH++ QS+H K+LSE+
Sbjct: 227 QQDGSHNKSLLKLAKLDFNLLQSLHRKELSEI 258
Score = 39.3 bits (90), Expect(2) = 3e-25
Identities = 18/27 (66%), Positives = 23/27 (85%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNL 170
LKLID+IQ LG+ +HFE EIEEAL ++
Sbjct: 77 LKLIDSIQLLGLAHHFEREIEEALKDM 103
>emb|CBI31305.3| unnamed protein product [Vitis vinifera]
Length = 645
Score = 196 bits (499), Expect(2) = 5e-71
Identities = 94/218 (43%), Positives = 134/218 (61%)
Frame = +1
Query: 1150 KFPEYMKIIYKXXXXXXXXXXXXMKRQGKSYCADYAIEAIKVMIRDYLVEVKWREEKYKP 1329
+ PEYMK+ Y M+++G Y YA E +K +R Y E KW E++ P
Sbjct: 428 QLPEYMKLCYAALLDVYKEIEEEMEKEGNQYRVHYAKEVMKNQVRAYFAEAKWLHEEHVP 487
Query: 1330 TSEEYMRVGTASCAYISLILISFIGMGDLATKEALEWVLSQPDMVKSTLLISRLSNDIAG 1509
T EEY+RV S Y L S +GMG++ATKEA +WV S P ++ S+ I+RL +DI+
Sbjct: 488 TIEEYLRVALVSSGYCMLATTSLVGMGEIATKEAFDWVTSDPKIMSSSNFIARLMDDISS 547
Query: 1510 HELERERDHIPSIVECYMEEHNVTKENAVREFKKQIEEGWKDINEGFLKPTEFPAPLLYC 1689
H+ E++R+H+ S +ECYM+++ V++E A EF+KQIE W DIN+ LKPT P PLL
Sbjct: 548 HKFEQKREHVASAIECYMKQYGVSEEQAYSEFRKQIENAWMDINQECLKPTAVPMPLLAR 607
Query: 1690 ILNFARLIENIYFKDQDWYTHVGPEMQTFVRLLLIDPV 1803
+LN R + IY K+QD YTHVG M+ + I+P+
Sbjct: 608 VLNLTRAADVIY-KEQDSYTHVGKVMKNNIAAFFINPI 644
Score = 100 bits (250), Expect(2) = 5e-71
Identities = 45/75 (60%), Positives = 59/75 (78%)
Frame = +2
Query: 911 MSRWWKGFGLSTKLTFARDRLVETYYWVLAVFFEPKYVVSRNISTKVQALVSIVDDAFDA 1090
++RWWK +TKL FARDRLVE Y+W+L V+FEP+Y+ +R I TKV A+ SI+DD +DA
Sbjct: 345 LARWWKELDFATKLPFARDRLVEGYFWILGVYFEPQYLRARRILTKVIAMTSILDDIYDA 404
Query: 1091 YGTFQELSLFTQAIE 1135
YG +EL LFT+AIE
Sbjct: 405 YGNPEELKLFTEAIE 419
Score = 103 bits (257), Expect(2) = 9e-28
Identities = 61/154 (39%), Positives = 89/154 (57%), Gaps = 3/154 (1%)
Frame = +1
Query: 196 DENHDIYPTALGFHLLRQHGYRVSCEIFKKFEDGEEGGDWR---VEEAKGLLEFFEATYM 366
D + D+Y ALGF LLRQ GY +SC I KKF D E G ++ + +GLL +EA ++
Sbjct: 198 DIDGDLYNVALGFRLLRQQGYSISCGILKKFTD--ERGRFKEVLITNVRGLLGLYEAAHL 255
Query: 367 RVRGEEVLERASAYTKSELTKLLPSLTDTNIPLAQEINHALHGHSKRRGLTRLEARKYIA 546
RV GE++L A +T + L ++ SL PLA+++ HAL+ R+GL R+EAR YI+
Sbjct: 256 RVHGEDILAEALTFTTTHLKAMVESL---GYPLAEQVVHALN-RPIRKGLERIEARWYIS 311
Query: 547 VYEQYPSHHRCXXXXXXXXXXXXQSMHMKQLSEL 648
VY+ H + QS+H ++LS L
Sbjct: 312 VYQDEAFHDKTLLELAKLDFNLVQSLHREELSNL 345
Score = 49.3 bits (116), Expect(2) = 9e-28
Identities = 22/34 (64%), Positives = 28/34 (82%)
Frame = +3
Query: 90 LKLIDAIQRLGVEYHFEEEIEEALLNLSQNFHFL 191
L IDA+QRLGV YHFE+EIEEAL ++ ++FH L
Sbjct: 163 LNFIDAVQRLGVAYHFEQEIEEALQHIYESFHDL 196