BLASTX nr result
ID: Mentha27_contig00020667
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha27_contig00020667
(1595 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU23312.1| hypothetical protein MIMGU_mgv1a026412mg [Mimulus... 766 0.0
gb|EPS72161.1| hypothetical protein M569_02597 [Genlisea aurea] 694 0.0
ref|XP_006365966.1| PREDICTED: diphthamide biosynthesis protein ... 643 0.0
ref|XP_004241760.1| PREDICTED: diphthamide biosynthesis protein ... 640 0.0
ref|XP_007041598.1| Diphthamide synthesis DPH2 family protein [T... 617 e-174
ref|XP_007157033.1| hypothetical protein PHAVU_002G038000g [Phas... 597 e-168
ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein ... 592 e-166
ref|XP_004511457.1| PREDICTED: diphthamide biosynthesis protein ... 592 e-166
ref|XP_006280451.1| hypothetical protein CARUB_v10026383mg [Caps... 589 e-165
ref|XP_002325444.1| diphthamide synthesis DPH2 family protein [P... 588 e-165
dbj|BAB10164.1| unnamed protein product [Arabidopsis thaliana] 586 e-165
ref|NP_201009.1| diphthamide synthesis DPH2 family protein [Arab... 586 e-165
ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago t... 586 e-164
ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [... 585 e-164
ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein ... 583 e-164
ref|XP_002864766.1| diphthamide synthesis DPH2 family protein [A... 581 e-163
ref|XP_006493302.1| PREDICTED: diphthamide biosynthesis protein ... 580 e-163
ref|XP_006394442.1| hypothetical protein EUTSA_v10004160mg [Eutr... 580 e-163
ref|XP_006592079.1| PREDICTED: diphthamide biosynthesis protein ... 580 e-163
ref|XP_003533490.1| PREDICTED: diphthamide biosynthesis protein ... 576 e-162
>gb|EYU23312.1| hypothetical protein MIMGU_mgv1a026412mg [Mimulus guttatus]
Length = 459
Score = 766 bits (1979), Expect = 0.0
Identities = 381/458 (83%), Positives = 410/458 (89%), Gaps = 11/458 (2%)
Frame = +2
Query: 44 MAETSA--ANNQLVEQ------PPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYN 199
MAET+ +++ LV+Q PPPSKPRPKRFV++QIPDSILNDAALNAA+SLLPANY+
Sbjct: 1 MAETTVKPSSDLLVQQQQQQQPPPPSKPRPKRFVKNQIPDSILNDAALNAAISLLPANYS 60
Query: 200 FEIHKIIWRVRSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVD 379
FEIHKIIWR RS NATRVALQFPEGLLMYSLVISDILTTFAAVTHCF+LGDVTYGACCVD
Sbjct: 61 FEIHKIIWRARSTNATRVALQFPEGLLMYSLVISDILTTFAAVTHCFVLGDVTYGACCVD 120
Query: 380 DLSARALDVHLLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNF 559
DLSARALD HLLVHFGHSCLVPIDSTT+PVLYIFVEIAINTRKLLHEL +NF+PD+I++F
Sbjct: 121 DLSARALDAHLLVHFGHSCLVPIDSTTIPVLYIFVEIAINTRKLLHELNHNFTPDQINSF 180
Query: 560 VMAGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVII 739
VMAGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQ C GKDDVII
Sbjct: 181 VMAGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQTCPGKDDVII 240
Query: 740 FVADGRFHLEAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGI 919
FVADGRFHLEAFMIANP+I+ FRFDPYLGKLFLEEYDH+GMK +RR AIERA GAKTWGI
Sbjct: 241 FVADGRFHLEAFMIANPEIRAFRFDPYLGKLFLEEYDHEGMKGDRRKAIERAMGAKTWGI 300
Query: 920 VLGTLGRQGNPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLS 1099
VLGTLGRQGN +++ RLE KMKDKGLDYMVVLISELSPKKIE+F DAVDAWVQIACPRLS
Sbjct: 301 VLGTLGRQGNTMIMKRLEEKMKDKGLDYMVVLISELSPKKIELFGDAVDAWVQIACPRLS 360
Query: 1100 IDWGDAFKKPLLTTFEAEIALGDLPGWWERK-LVCNDSSECAKKEGCCGNQN--VQKGVD 1270
IDWGDAFKKPLLT FEAEIALGDL GWW+RK CN SE + E CCGN N V++GVD
Sbjct: 361 IDWGDAFKKPLLTAFEAEIALGDLDGWWQRKSRCCNGGSESTRNEECCGNGNGDVREGVD 420
Query: 1271 YPMDYYAQDGGEWNSSYSKKSARDRVKKDQCCNGSSVK 1384
YPMDYYAQDGG WNS YSKK AR K +QCCN S K
Sbjct: 421 YPMDYYAQDGGGWNSCYSKKPARVSRKNNQCCNNSCEK 458
>gb|EPS72161.1| hypothetical protein M569_02597 [Genlisea aurea]
Length = 440
Score = 694 bits (1791), Expect = 0.0
Identities = 335/431 (77%), Positives = 379/431 (87%), Gaps = 1/431 (0%)
Frame = +2
Query: 98 KPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPEGL 277
+P PKRFV+SQIP+SILNDAALNAAV++LPANYNFEIHKI+WR RS+NATRVALQFPEGL
Sbjct: 18 RPPPKRFVKSQIPESILNDAALNAAVAVLPANYNFEIHKIVWRARSSNATRVALQFPEGL 77
Query: 278 LMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDST 457
LMYSLVISDILT FA VTHCF+LGDVTYGACCVDDLS RAL VHLLVHFGHSCLVPI +T
Sbjct: 78 LMYSLVISDILTAFAGVTHCFVLGDVTYGACCVDDLSCRALAVHLLVHFGHSCLVPIGNT 137
Query: 458 TVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGFRV 637
TVPVLYIFVEI INTR LL EL YNF+P+K+SNF+MAGTIQF NAIRAVKPELENLGFRV
Sbjct: 138 TVPVLYIFVEIGINTRNLLQELDYNFNPEKVSNFIMAGTIQFCNAIRAVKPELENLGFRV 197
Query: 638 LIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRFDP 817
L+PQSKPLSAGEVLGCTAP+VKLQDC GK+D+IIFV+DGRFHLEAFMIANP +K FR+DP
Sbjct: 198 LMPQSKPLSAGEVLGCTAPTVKLQDCPGKEDIIIFVSDGRFHLEAFMIANPGVKAFRYDP 257
Query: 818 YLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKMKDKGL 997
YLGKLFLEEYD++GMK++RR AIERA GAK+WGIVLGTLGRQG+P+++ RLE KM++KGL
Sbjct: 258 YLGKLFLEEYDYEGMKQDRRKAIERAMGAKSWGIVLGTLGRQGSPMLVERLEKKMQEKGL 317
Query: 998 DYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGDLPG 1177
DY+ VL+SELSPKKI++F D+VDAW+QIACPRLSIDWGDAFKKPLLT+FEAEIALGD PG
Sbjct: 318 DYITVLLSELSPKKIDLFGDSVDAWIQIACPRLSIDWGDAFKKPLLTSFEAEIALGDRPG 377
Query: 1178 WWERKLVCNDSSECAKKEGCCGNQNVQKGVDYPMDYYAQDGGEWNSSY-SKKSARDRVKK 1354
WW+ K + K C N+N ++ VDYPMDYY+QDGGEWNSSY KKS R K
Sbjct: 378 WWDMKPM--------KSSNGCRNRNEEECVDYPMDYYSQDGGEWNSSYLKKKSGRASGKI 429
Query: 1355 DQCCNGSSVKS 1387
QCC S S
Sbjct: 430 GQCCTSSCTGS 440
>ref|XP_006365966.1| PREDICTED: diphthamide biosynthesis protein 1-like [Solanum
tuberosum]
Length = 481
Score = 643 bits (1659), Expect = 0.0
Identities = 316/463 (68%), Positives = 376/463 (81%), Gaps = 15/463 (3%)
Frame = +2
Query: 35 PPTMAETSAANNQLVEQP--PPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEI 208
P A T+ A + +QP ++ RPKRFV++Q+PDSILNDAALNAA+SLLP NYNFEI
Sbjct: 13 PTPAAATTTAVDAGDQQPLQQSTRSRPKRFVKNQMPDSILNDAALNAAISLLPQNYNFEI 72
Query: 209 HKIIWRVRSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLS 388
HK +WRVR++ A RVALQFPEGLLMYSL+ISDIL+TF + THCFILGDVT+GACCVDDLS
Sbjct: 73 HKCVWRVRTSGAKRVALQFPEGLLMYSLIISDILSTFTSATHCFILGDVTFGACCVDDLS 132
Query: 389 ARALDVHLLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNF-SPDKISNFVM 565
A AL LL+HFGHSCLVPIDSTT+P LYIFVEI+I+ +LL+ELK NF + D N +M
Sbjct: 133 AGALSADLLIHFGHSCLVPIDSTTIPCLYIFVEISIDVHRLLNELKLNFYNSDTYDNIIM 192
Query: 566 AGTIQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFV 745
AGTIQF++AIRAVKPELE +GF +LIPQ+KPLSAGEVLGCTAPSVK + G++ V++FV
Sbjct: 193 AGTIQFASAIRAVKPELEKMGFSILIPQAKPLSAGEVLGCTAPSVKNRFSDGENVVLVFV 252
Query: 746 ADGRFHLEAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVL 925
ADGRFHLEAFMIANP IKT+R+DP++GKLF+EEYDHKGMKE R+ AIE+A+G K WGIVL
Sbjct: 253 ADGRFHLEAFMIANPGIKTYRYDPFIGKLFVEEYDHKGMKEERKRAIEKARGVKNWGIVL 312
Query: 926 GTLGRQGNPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSID 1105
GTLGRQGNP +L+RLE KM +KG+ + VVL+SE+SP +I +F DAVDAW+QIACPRLSID
Sbjct: 313 GTLGRQGNPRILDRLEKKMSEKGMTWTVVLMSEISPTRIALFEDAVDAWIQIACPRLSID 372
Query: 1106 WGDAFKKPLLTTFEAEIALGDLPGWWERK------LVCNDSSECAKKEGC--CGNQ---- 1249
WGDAFKKPLLT FEAEIALGDL GWWERK + C++ +C+K E C C N
Sbjct: 373 WGDAFKKPLLTPFEAEIALGDLSGWWERKTSVNSDICCDEDMKCSKNESCGACDNDGEKV 432
Query: 1250 NVQKGVDYPMDYYAQDGGEWNSSYSKKSARDRVKKDQCCNGSS 1378
+ VDYPMDYYAQDGGEWNS YSKK AR + Q CNG+S
Sbjct: 433 KEESQVDYPMDYYAQDGGEWNSCYSKKPARLSHRNSQSCNGAS 475
>ref|XP_004241760.1| PREDICTED: diphthamide biosynthesis protein 1-like [Solanum
lycopersicum]
Length = 483
Score = 640 bits (1652), Expect = 0.0
Identities = 316/453 (69%), Positives = 373/453 (82%), Gaps = 13/453 (2%)
Frame = +2
Query: 59 AANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSA 238
A + Q ++Q ++PRPKRFV++QIPDSILNDAALNAA+SLLP NYNFEIHK +WRVR++
Sbjct: 27 AGDQQSLQQS--TRPRPKRFVKNQIPDSILNDAALNAAISLLPQNYNFEIHKCVWRVRTS 84
Query: 239 NATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLV 418
+A RVALQFPEGLLMYSL+ISDIL+TF + THCFILGDVT+GACCVDDLSA AL LL+
Sbjct: 85 SAKRVALQFPEGLLMYSLIISDILSTFTSATHCFILGDVTFGACCVDDLSAGALSADLLI 144
Query: 419 HFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNF-SPDKISNFVMAGTIQFSNAI 595
HFGHSCLVPIDSTT+P LYIFVEI+I+ +LL+ELK NF + D N +MAGTIQF++AI
Sbjct: 145 HFGHSCLVPIDSTTIPCLYIFVEISIDVHRLLNELKLNFYNSDTYDNIIMAGTIQFASAI 204
Query: 596 RAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAF 775
RAVKPELE LGF +LIPQ+KPLSAGEVLGCTAPSVK + G++ V+IFVADGRFHLEAF
Sbjct: 205 RAVKPELEKLGFSILIPQAKPLSAGEVLGCTAPSVKNRFSDGENVVLIFVADGRFHLEAF 264
Query: 776 MIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPV 955
MIANP IKT+R+DP++GKLF+EEYDHKGMKE R+ AIE+A+GAK WGIVLGTLGRQGNP
Sbjct: 265 MIANPGIKTYRYDPFIGKLFVEEYDHKGMKEERKRAIEKARGAKNWGIVLGTLGRQGNPR 324
Query: 956 VLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLL 1135
+L+RLE KM +KG+ + VVL+SE+SP +I +F DAVDAW+QIACPRLSIDWGDAFKKPLL
Sbjct: 325 ILDRLEKKMSEKGMTWTVVLMSEISPTRIALFEDAVDAWIQIACPRLSIDWGDAFKKPLL 384
Query: 1136 TTFEAEIALGDLPGWWERKL-----VCNDS-SECAKKEGC--CGNQ----NVQKGVDYPM 1279
T FEAEIALGDL GWWER +C D +C+K E C C + VDYPM
Sbjct: 385 TPFEAEIALGDLSGWWERTTSKNSDICRDEVMKCSKNESCGACDKSGEEVKEETQVDYPM 444
Query: 1280 DYYAQDGGEWNSSYSKKSARDRVKKDQCCNGSS 1378
DYYAQDGGEWNS YSKK AR + Q CNG++
Sbjct: 445 DYYAQDGGEWNSCYSKKPARLSQRNSQSCNGAT 477
>ref|XP_007041598.1| Diphthamide synthesis DPH2 family protein [Theobroma cacao]
gi|508705533|gb|EOX97429.1| Diphthamide synthesis DPH2
family protein [Theobroma cacao]
Length = 458
Score = 617 bits (1590), Expect = e-174
Identities = 303/417 (72%), Positives = 349/417 (83%), Gaps = 2/417 (0%)
Frame = +2
Query: 95 SKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPEG 274
SKP PKRFV++QIPDSILNDA+LNAA+SLLP+NYNFEIHK +WRVRS A RVALQFPEG
Sbjct: 30 SKPAPKRFVKNQIPDSILNDASLNAAISLLPSNYNFEIHKCVWRVRSTGAKRVALQFPEG 89
Query: 275 LLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDS 454
LLMYSLVISDILT+FA VTHCFILGDVTYGACC+DDLSA AL LL+H+GHSCLVPIDS
Sbjct: 90 LLMYSLVISDILTSFADVTHCFILGDVTYGACCIDDLSATALGADLLIHYGHSCLVPIDS 149
Query: 455 TTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGFR 634
T +P LY+FVEI IN L++ +++N + K S V+AGTIQFS+AIR+ KPELE GFR
Sbjct: 150 TKIPCLYVFVEIKINVDGLINTIEHNLTSSKES-VVLAGTIQFSSAIRSAKPELEKRGFR 208
Query: 635 VLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRFD 814
VLIPQSKPLSAGEVLGCTAP + A + V +FVADGRFHLEAFMIANP IK FR+D
Sbjct: 209 VLIPQSKPLSAGEVLGCTAPKIGTVANAN-ETVAVFVADGRFHLEAFMIANPGIKAFRYD 267
Query: 815 PYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKMKDKG 994
PYLGKLFLEEYDHKGM+E RR AI++AK AK+WGIVLGTLGRQGNP VL RLEGKM++KG
Sbjct: 268 PYLGKLFLEEYDHKGMRETRRRAIQKAKRAKSWGIVLGTLGRQGNPRVLERLEGKMREKG 327
Query: 995 LDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGDLP 1174
DY ++L+SE+SP +I +F DAVDAW+QIACPRLSIDWGDAF KPLLTTFEAEIALG++
Sbjct: 328 FDYTIILMSEISPTRIALFEDAVDAWIQIACPRLSIDWGDAFVKPLLTTFEAEIALGEIS 387
Query: 1175 GWWERKLVCNDSSECAKKEGCCGNQNVQ--KGVDYPMDYYAQDGGEWNSSYSKKSAR 1339
GWWER C + C + + CCG + + +G DYPMDYYAQDGGEWNSSY KK AR
Sbjct: 388 GWWERD-GCGNGLSCGESKSCCGCRGREDFRG-DYPMDYYAQDGGEWNSSYVKKLAR 442
>ref|XP_007157033.1| hypothetical protein PHAVU_002G038000g [Phaseolus vulgaris]
gi|561030448|gb|ESW29027.1| hypothetical protein
PHAVU_002G038000g [Phaseolus vulgaris]
Length = 461
Score = 597 bits (1539), Expect = e-168
Identities = 281/420 (66%), Positives = 342/420 (81%), Gaps = 3/420 (0%)
Frame = +2
Query: 89 PPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFP 268
P KP+PKRFV++Q+PDSILND LNAA+S+LP+NYNFEIHK +WRV S A RVALQFP
Sbjct: 24 PKPKPKPKRFVKNQVPDSILNDPLLNAAISVLPSNYNFEIHKSVWRVLSTGAKRVALQFP 83
Query: 269 EGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPI 448
EGLLMYSL +SDI TTFAAVTHC+ILGDVTYGACCVDD +A ALD LL+H+GHSCLVPI
Sbjct: 84 EGLLMYSLSLSDIFTTFAAVTHCYILGDVTYGACCVDDFAAAALDADLLIHYGHSCLVPI 143
Query: 449 DSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLG 628
D+TT+P LY+FV+I I+ + ++ N + V+AGTIQF++AIRA KP+LE LG
Sbjct: 144 DATTIPCLYVFVDIKIDVPHFVDTVRLNLE-SRPQTLVVAGTIQFASAIRAAKPQLEELG 202
Query: 629 FRVLIPQSKPLSAGEVLGCTAPSVKLQD--CAGKDDVIIFVADGRFHLEAFMIANPDIKT 802
FRVL+PQSKPLSAGEVLGCTAP V C + V++FVADGRFHLEAFMIANP I+
Sbjct: 203 FRVLVPQSKPLSAGEVLGCTAPRVSSNSLGCGVGESVLVFVADGRFHLEAFMIANPGIRA 262
Query: 803 FRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKM 982
FR+DPY+GKLFLEEYDH GMK +R+NAI +A+ A++WG+VLGTLGRQGNP +L RLE KM
Sbjct: 263 FRYDPYMGKLFLEEYDHVGMKRSRKNAILKAREARSWGVVLGTLGRQGNPRILERLEKKM 322
Query: 983 KDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIAL 1162
+++GLDY VVL+SELSP ++ +F D+VD+W+QIACPRLSIDWG+AF KP+LT+FEA++AL
Sbjct: 323 RERGLDYTVVLMSELSPGRVALFEDSVDSWIQIACPRLSIDWGEAFVKPVLTSFEADVAL 382
Query: 1163 GDLPGWWERKLVCNDSSECAKKEG-CCGNQNVQKGVDYPMDYYAQDGGEWNSSYSKKSAR 1339
G +PGWWE+ VC++S C K+ G CC G DYPMDYYAQDGGEWNSSY K S R
Sbjct: 383 GLIPGWWEKNEVCDESVGCGKRSGSCCEGDAKDFGGDYPMDYYAQDGGEWNSSYMKNSTR 442
>ref|XP_004136856.1| PREDICTED: diphthamide biosynthesis protein 1-like [Cucumis sativus]
gi|449478949|ref|XP_004155462.1| PREDICTED: diphthamide
biosynthesis protein 1-like [Cucumis sativus]
Length = 462
Score = 592 bits (1527), Expect = e-166
Identities = 291/448 (64%), Positives = 351/448 (78%), Gaps = 8/448 (1%)
Frame = +2
Query: 47 AETSAANNQLVEQPPP-SKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIW 223
+E + + ++PPP S+P PKRFV++QIPDSILND ALNAA+SLLP+NYNFE+HK +W
Sbjct: 10 SELAVLSQSTEDKPPPRSRPPPKRFVKNQIPDSILNDPALNAAISLLPSNYNFEVHKCVW 69
Query: 224 RVRSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALD 403
R+ S++A+RVALQ PEGLLMYSL++SDILTTF +HCF+LGDVT+GACCVDDLSA AL
Sbjct: 70 RILSSSASRVALQLPEGLLMYSLILSDILTTFGKASHCFVLGDVTFGACCVDDLSASALH 129
Query: 404 VHLLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQF 583
LLVH+GHSCLVPIDSTT+P LY+FV+IAI+ +L++ ++ N +N V+AGTIQF
Sbjct: 130 ADLLVHYGHSCLVPIDSTTIPCLYVFVDIAIDVPRLVNTVRLNVDCLDSTNLVLAGTIQF 189
Query: 584 SNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGK-DDVIIFVADGRF 760
++AIRA K ELE G RVLIPQS+PLSAGEVLGCTAP V + V IFVADGRF
Sbjct: 190 ASAIRASKMELERHGIRVLIPQSRPLSAGEVLGCTAPKVSTAAFGNNGETVAIFVADGRF 249
Query: 761 HLEAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGR 940
HLEAFMIANP I+ FR+DPY+GKL LEEYDH+GMK +R+ AI +AK A WGIVLGTLGR
Sbjct: 250 HLEAFMIANPGIRVFRYDPYIGKLLLEEYDHEGMKASRKGAILKAKEATNWGIVLGTLGR 309
Query: 941 QGNPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAF 1120
QGNP +L RLE KMK KG DY VVL+SE+SP ++ +F D+VDAW+QIACPRLSIDWGDAF
Sbjct: 310 QGNPKILERLEEKMKSKGFDYTVVLMSEISPYRVALFEDSVDAWIQIACPRLSIDWGDAF 369
Query: 1121 KKPLLTTFEAEIALGDLPGWWER----KLVCNDSSECAKKEGCCGNQ--NVQKGVDYPMD 1282
KPLLT FEAE+ALG +PGWWER KL CND EC GC G++ + +YPMD
Sbjct: 370 AKPLLTPFEAEVALGLIPGWWERTSSAKLSCNDCREC----GCNGDEINKAEAEGEYPMD 425
Query: 1283 YYAQDGGEWNSSYSKKSARDRVKKDQCC 1366
YYAQDGGEWNSSY KK V++D C
Sbjct: 426 YYAQDGGEWNSSYVKKKVSRPVRRDVSC 453
>ref|XP_004511457.1| PREDICTED: diphthamide biosynthesis protein 1-like [Cicer arietinum]
Length = 465
Score = 592 bits (1525), Expect = e-166
Identities = 294/450 (65%), Positives = 345/450 (76%), Gaps = 13/450 (2%)
Frame = +2
Query: 29 SRPPTMAETSAANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEI 208
S P A T N V K +PKRF++SQIPDSILND LNAA+S+LP+NYNFE+
Sbjct: 6 SDKPNNANT---NTVTVSVATKEKSKPKRFIKSQIPDSILNDPLLNAAISILPSNYNFEV 62
Query: 209 HKIIWRVRSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLS 388
HK +WRV S A RVALQFPEGLLMYSL++SDI TTFA VTHC++LGDVTYGACC+DD S
Sbjct: 63 HKCVWRVLSTGAKRVALQFPEGLLMYSLILSDIFTTFAGVTHCYVLGDVTYGACCIDDFS 122
Query: 389 ARALDVHLLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMA 568
A AL LL+H+GHSCLVPIDSTT+P LY+FV+I I+ L+ ++ N ++A
Sbjct: 123 AVALSADLLIHYGHSCLVPIDSTTIPCLYVFVDIKIDVDHLVETVRLNLE-SLGKTLIIA 181
Query: 569 GTIQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSV--KLQDCAGKDDVIIF 742
GTIQF++ IRAVKPELE LGF+V IPQSKPLSAGEVLGCTAP V L DC + V++F
Sbjct: 182 GTIQFASGIRAVKPELEKLGFKVFIPQSKPLSAGEVLGCTAPKVAKSLDDCG--ESVLVF 239
Query: 743 VADGRFHLEAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIV 922
V+DGRFHLEAFMIANP IKTFR+DPY+GKLFLEEYDH GMK +R+NAI +AK A+ WG+V
Sbjct: 240 VSDGRFHLEAFMIANPGIKTFRYDPYIGKLFLEEYDHVGMKGSRKNAILKAKEARNWGVV 299
Query: 923 LGTLGRQGNPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSI 1102
LGTLGRQGNP +L RLE KM++KG DY VVL+SELSPK+I +F D+VDAW+QIACPRLSI
Sbjct: 300 LGTLGRQGNPKILERLEKKMREKGFDYTVVLMSELSPKRISLFEDSVDAWIQIACPRLSI 359
Query: 1103 DWGDAFKKPLLTTFEAEIALGDLPGWWERKLV----CNDSSECAKKEGCCGN-------Q 1249
DWGDAF KP+LT FEAEIALG +PGWWE+ V C D C K + CC N +
Sbjct: 360 DWGDAFVKPVLTPFEAEIALGLIPGWWEKTQVQKQGCEDVIGCNKSD-CCSNGSCGDAKE 418
Query: 1250 NVQKGVDYPMDYYAQDGGEWNSSYSKKSAR 1339
G DYPMDYYAQDGGEWNSSY KKS R
Sbjct: 419 TNDFGGDYPMDYYAQDGGEWNSSYVKKSTR 448
>ref|XP_006280451.1| hypothetical protein CARUB_v10026383mg [Capsella rubella]
gi|482549155|gb|EOA13349.1| hypothetical protein
CARUB_v10026383mg [Capsella rubella]
Length = 453
Score = 589 bits (1518), Expect = e-165
Identities = 292/446 (65%), Positives = 351/446 (78%), Gaps = 16/446 (3%)
Frame = +2
Query: 50 ETSAANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRV 229
E S NN +P K PKRF+++QIPDS+LNDA+LNAA+S+LP+ Y FE+HK +WR+
Sbjct: 2 ELSDLNNGKSTKP---KQPPKRFIKNQIPDSVLNDASLNAAISILPSIYQFEVHKCVWRI 58
Query: 230 RSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVH 409
+S+NA R+ALQ PEGLLMY+L +SDI T+FA +HCF+LGDVTYGACCVDD SA AL
Sbjct: 59 KSSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGAD 118
Query: 410 LLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSN 589
LL+H+GHSCLVPIDST +P LY+FVEI I+ + LL+ + N S D N ++AGTIQF++
Sbjct: 119 LLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHLNLSSDA-KNIILAGTIQFTS 177
Query: 590 AIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVK-LQDCAGKDDVIIFVADGRFHL 766
AIRAVKPELE GF VLIPQSKPLSAGEVLGCTAP +K ++DC KD V++FVADGRFHL
Sbjct: 178 AIRAVKPELEKQGFNVLIPQSKPLSAGEVLGCTAPKIKTVEDC--KDQVLVFVADGRFHL 235
Query: 767 EAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQG 946
EAFMIANP IK FR+DPYLGKLFLEEYDHKGM+E RR AI RA+ AKTWGIVLGTLGRQG
Sbjct: 236 EAFMIANPKIKAFRYDPYLGKLFLEEYDHKGMRETRRRAIARARDAKTWGIVLGTLGRQG 295
Query: 947 NPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKK 1126
NP +L RLE KM++KG+D VVL+SELSP ++ +F D+VDAWVQIACPRLSIDWG+AF K
Sbjct: 296 NPKILERLEKKMREKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLK 355
Query: 1127 PLLTTFEAEIALGDLPGWWERKLVC--NDSSECAKKEG----CCGNQNVQKGV------- 1267
PLLTTFEAEIALG + GWWE+ + S C K++ C N+NV+
Sbjct: 356 PLLTTFEAEIALGFIRGWWEKDTSSRGDSLSGCCKEKKETSCACSNENVENDKKDDDGAL 415
Query: 1268 --DYPMDYYAQDGGEWNSSYSKKSAR 1339
DYPMDYYAQ+GGEWNSSY KKS+R
Sbjct: 416 DGDYPMDYYAQEGGEWNSSYLKKSSR 441
>ref|XP_002325444.1| diphthamide synthesis DPH2 family protein [Populus trichocarpa]
gi|222862319|gb|EEE99825.1| diphthamide synthesis DPH2
family protein [Populus trichocarpa]
Length = 428
Score = 588 bits (1516), Expect = e-165
Identities = 291/428 (67%), Positives = 340/428 (79%), Gaps = 5/428 (1%)
Frame = +2
Query: 71 QLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATR 250
Q ++ SKP PKRF++SQIPDSILND +LNA++SLLP+NYNFEIHK IWRVRS NA R
Sbjct: 2 QAIQLNQKSKPTPKRFIKSQIPDSILNDPSLNASISLLPSNYNFEIHKCIWRVRSTNAKR 61
Query: 251 VALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGH 430
+ALQ PEGLLMYSL++SDI T FA VTHCF+LGDVTYGACCVDDLSA ALD LL+H+GH
Sbjct: 62 IALQLPEGLLMYSLILSDIFTAFADVTHCFVLGDVTYGACCVDDLSALALDADLLIHYGH 121
Query: 431 SCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKP 610
SCL+PID+T +P LY+FVEI I+ +L++ + NF K N V+AGTIQF++AIR KP
Sbjct: 122 SCLIPIDATKIPCLYVFVEIKIDVSRLINTISLNFGTHK-RNIVLAGTIQFASAIREAKP 180
Query: 611 ELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAG-----KDDVIIFVADGRFHLEAF 775
ELEN GF VLIPQSKPLSAGEVLGCTAP + ++ V++FVADGRFHLEAF
Sbjct: 181 ELENNGFVVLIPQSKPLSAGEVLGCTAPKMSKNVVGNLGFSEEEMVLVFVADGRFHLEAF 240
Query: 776 MIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPV 955
MIANP IK FR+DPYLGKLFLEEYDHKGMKE R+ AIERAKGA++WG+VLGTLGRQGNP
Sbjct: 241 MIANPGIKAFRYDPYLGKLFLEEYDHKGMKEVRKKAIERAKGARSWGVVLGTLGRQGNPR 300
Query: 956 VLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLL 1135
+L RLE KM +KG DYMVVL+SE+SP +I +F D+VDAW+QIACPRLSIDWGDAFKKPLL
Sbjct: 301 ILERLEKKMGEKGFDYMVVLMSEISPGRIALFEDSVDAWIQIACPRLSIDWGDAFKKPLL 360
Query: 1136 TTFEAEIALGDLPGWWERKLVCNDSSECAKKEGCCGNQNVQKGVDYPMDYYAQDGGEWNS 1315
T FEAEIALGDL G CN ++ +K+ G +YPMDYYAQDGGEWNS
Sbjct: 361 TPFEAEIALGDLAG-------CNGDAKGVEKD---------FGGEYPMDYYAQDGGEWNS 404
Query: 1316 SYSKKSAR 1339
SY KK R
Sbjct: 405 SYVKKPTR 412
>dbj|BAB10164.1| unnamed protein product [Arabidopsis thaliana]
Length = 468
Score = 586 bits (1511), Expect = e-165
Identities = 292/446 (65%), Positives = 350/446 (78%), Gaps = 16/446 (3%)
Frame = +2
Query: 50 ETSAANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRV 229
E S NN +P K PKRF+++QIPDSIL DA+LNAA+S+LP+ Y+FE+HK +WR+
Sbjct: 17 ELSDPNNGKTNKP---KQPPKRFIKNQIPDSILGDASLNAAISILPSIYHFEVHKCVWRI 73
Query: 230 RSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVH 409
+++NA R+ALQ PEGLLMY+L +SDI T+FA +HCF+LGDVTYGACCVDD SA AL
Sbjct: 74 KTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGAD 133
Query: 410 LLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSN 589
LL+H+GHSCLVPIDST +P LY+FVEI I+ + LL+ + N + D + N ++AGTIQF++
Sbjct: 134 LLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHLNLASD-VKNIILAGTIQFTS 192
Query: 590 AIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVK-LQDCAGKDDVIIFVADGRFHL 766
AIRAVKPELE GF VLIPQSKPLSAGEVLGCTAP VK ++DC KD V++FVADGRFHL
Sbjct: 193 AIRAVKPELEKQGFNVLIPQSKPLSAGEVLGCTAPKVKTVEDC--KDQVLVFVADGRFHL 250
Query: 767 EAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQG 946
EAFMIANP IK FR+DPYLGKLFLEEYDHKGM+E R AI RA+ AKTWGIVLGTLGRQG
Sbjct: 251 EAFMIANPKIKAFRYDPYLGKLFLEEYDHKGMRETRMRAIARAREAKTWGIVLGTLGRQG 310
Query: 947 NPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKK 1126
NP +L RLE KM +KG+D VVL+SELSP ++ +F D+VDAWVQIACPRLSIDWG+AF K
Sbjct: 311 NPKILERLEKKMMEKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLK 370
Query: 1127 PLLTTFEAEIALGDLPGWWER--KLVCNDSSECAKKEG----CCGNQNVQKGV------- 1267
PLLTTFEAEIALG + GWWE N SS C K++ C N+NV+
Sbjct: 371 PLLTTFEAEIALGFIRGWWENDSSSRVNSSSGCCKEDKETSCACKNENVKDDKKDNDGAL 430
Query: 1268 --DYPMDYYAQDGGEWNSSYSKKSAR 1339
DYPMDYYAQ+GGEWNSSY KKS+R
Sbjct: 431 DGDYPMDYYAQEGGEWNSSYLKKSSR 456
>ref|NP_201009.1| diphthamide synthesis DPH2 family protein [Arabidopsis thaliana]
gi|20259528|gb|AAM13884.1| unknown protein [Arabidopsis
thaliana] gi|21436215|gb|AAM51395.1| unknown protein
[Arabidopsis thaliana] gi|110742463|dbj|BAE99150.1|
hypothetical protein [Arabidopsis thaliana]
gi|332010170|gb|AED97553.1| diphthamide synthesis DPH2
family protein [Arabidopsis thaliana]
Length = 453
Score = 586 bits (1511), Expect = e-165
Identities = 292/446 (65%), Positives = 350/446 (78%), Gaps = 16/446 (3%)
Frame = +2
Query: 50 ETSAANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRV 229
E S NN +P K PKRF+++QIPDSIL DA+LNAA+S+LP+ Y+FE+HK +WR+
Sbjct: 2 ELSDPNNGKTNKP---KQPPKRFIKNQIPDSILGDASLNAAISILPSIYHFEVHKCVWRI 58
Query: 230 RSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVH 409
+++NA R+ALQ PEGLLMY+L +SDI T+FA +HCF+LGDVTYGACCVDD SA AL
Sbjct: 59 KTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGAD 118
Query: 410 LLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSN 589
LL+H+GHSCLVPIDST +P LY+FVEI I+ + LL+ + N + D + N ++AGTIQF++
Sbjct: 119 LLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHLNLASD-VKNIILAGTIQFTS 177
Query: 590 AIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVK-LQDCAGKDDVIIFVADGRFHL 766
AIRAVKPELE GF VLIPQSKPLSAGEVLGCTAP VK ++DC KD V++FVADGRFHL
Sbjct: 178 AIRAVKPELEKQGFNVLIPQSKPLSAGEVLGCTAPKVKTVEDC--KDQVLVFVADGRFHL 235
Query: 767 EAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQG 946
EAFMIANP IK FR+DPYLGKLFLEEYDHKGM+E R AI RA+ AKTWGIVLGTLGRQG
Sbjct: 236 EAFMIANPKIKAFRYDPYLGKLFLEEYDHKGMRETRMRAIARAREAKTWGIVLGTLGRQG 295
Query: 947 NPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKK 1126
NP +L RLE KM +KG+D VVL+SELSP ++ +F D+VDAWVQIACPRLSIDWG+AF K
Sbjct: 296 NPKILERLEKKMMEKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLK 355
Query: 1127 PLLTTFEAEIALGDLPGWWER--KLVCNDSSECAKKEG----CCGNQNVQKGV------- 1267
PLLTTFEAEIALG + GWWE N SS C K++ C N+NV+
Sbjct: 356 PLLTTFEAEIALGFIRGWWENDSSSRVNSSSGCCKEDKETSCACKNENVKDDKKDNDGAL 415
Query: 1268 --DYPMDYYAQDGGEWNSSYSKKSAR 1339
DYPMDYYAQ+GGEWNSSY KKS+R
Sbjct: 416 DGDYPMDYYAQEGGEWNSSYLKKSSR 441
>ref|XP_003610873.1| Diphthamide biosynthesis protein [Medicago truncatula]
gi|355512208|gb|AES93831.1| Diphthamide biosynthesis
protein [Medicago truncatula]
Length = 575
Score = 586 bits (1510), Expect = e-164
Identities = 282/436 (64%), Positives = 341/436 (78%), Gaps = 12/436 (2%)
Frame = +2
Query: 68 NQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANAT 247
N + KP+PKRF+++QIPDSILND LNAA+S+LP+NY+FE+HK +WRVRS A
Sbjct: 11 NNTITVSVKEKPKPKRFIKNQIPDSILNDPLLNAAISILPSNYSFEVHKCVWRVRSTAAK 70
Query: 248 RVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFG 427
R+ALQFPEGLLMYSL++SDI TTFA VTHC++LGDVTYGACC+DD SA AL +L+H+G
Sbjct: 71 RIALQFPEGLLMYSLILSDIFTTFAGVTHCYVLGDVTYGACCIDDFSAIALGAEILIHYG 130
Query: 428 HSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKI--SNFVMAGTIQFSNAIRA 601
HSCLVPIDST +P LY+FV+I I+ L+ +K N ++AGTIQF++ IRA
Sbjct: 131 HSCLVPIDSTKIPCLYVFVDIKIDVDHLVDTVKLNSKDLGFMGKRVILAGTIQFASGIRA 190
Query: 602 VKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMI 781
VKPELE LGFRVL+PQSKPLSAGE+LGCTAP V + ++ V++FV+DGRFHLEAFMI
Sbjct: 191 VKPELEKLGFRVLVPQSKPLSAGEILGCTAPKVGKELDDKEESVLVFVSDGRFHLEAFMI 250
Query: 782 ANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVL 961
ANP +K FR+DPY+GKLFLEEYDH GMK +R+NAI +AK A+ WG+VLGTLGRQGNP +L
Sbjct: 251 ANPGVKAFRYDPYVGKLFLEEYDHVGMKGSRKNAILKAKEARNWGVVLGTLGRQGNPKIL 310
Query: 962 NRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTT 1141
RLE KM DKG DY VVL+SELSP +I +F D+VDAW+QIACPRLSIDWGDAF KP+LT
Sbjct: 311 ERLEMKMGDKGFDYTVVLMSELSPYRISLFEDSVDAWIQIACPRLSIDWGDAFVKPVLTP 370
Query: 1142 FEAEIALGDLPGWWERKLV----CNDSSECAKKEGC----CGNQNVQK--GVDYPMDYYA 1291
FEAEIALG +PGWWE+ V C D + C K + C CGN + G DYPMDYYA
Sbjct: 371 FEAEIALGLIPGWWEKTQVQKQGCEDVTGCNKSDCCSNGSCGNAKATEDFGGDYPMDYYA 430
Query: 1292 QDGGEWNSSYSKKSAR 1339
QDGGEWNSSY KK +R
Sbjct: 431 QDGGEWNSSYMKKPSR 446
>ref|XP_002529006.1| Diphthamide biosynthesis protein, putative [Ricinus communis]
gi|223531546|gb|EEF33376.1| Diphthamide biosynthesis
protein, putative [Ricinus communis]
Length = 434
Score = 585 bits (1507), Expect = e-164
Identities = 280/418 (66%), Positives = 331/418 (79%), Gaps = 2/418 (0%)
Frame = +2
Query: 92 PSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPE 271
P+KP PKRFV++QIPDSILNDA+LNAA++LLP+NYN EIHK +WR+RS R+ALQ PE
Sbjct: 12 PAKPPPKRFVKNQIPDSILNDASLNAAIALLPSNYNLEIHKCVWRIRSTGTKRLALQLPE 71
Query: 272 GLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPID 451
GLLMYSL++SDI T FA VTHCFILGDVTYGACC+DDLSA AL LL+H+GHSCLVPID
Sbjct: 72 GLLMYSLILSDIFTAFAGVTHCFILGDVTYGACCIDDLSALALGADLLIHYGHSCLVPID 131
Query: 452 STTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGF 631
+T +P LY+FVEI I+ L+ +K N K + ++AGTIQF++AIR KPELE LG
Sbjct: 132 ATKIPCLYVFVEIKIDVECLISTIKLNLKDYK--SVILAGTIQFASAIREAKPELERLGL 189
Query: 632 RVLIPQSKPLSAGEVLGCTAPSVKLQDCAG--KDDVIIFVADGRFHLEAFMIANPDIKTF 805
LIPQSKPLSAGEVLGCTAP + G + V++FVADGRFHLEAFMIANP I TF
Sbjct: 190 YALIPQSKPLSAGEVLGCTAPKISSNSMVGGGSETVVVFVADGRFHLEAFMIANPGISTF 249
Query: 806 RFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKMK 985
R+DPY+GKLFLEEYDH+GMKE R+ AI+ AK A+ WGIVLGTLGRQGNP +L+RLE KM+
Sbjct: 250 RYDPYMGKLFLEEYDHRGMKEVRKGAIQMAKEARNWGIVLGTLGRQGNPRILDRLEKKMR 309
Query: 986 DKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALG 1165
+KG Y VVL+SE+SP +I +F D +DAW+QIACPRLSIDWG+AFKKPLLT FEAEIALG
Sbjct: 310 EKGFSYTVVLMSEISPVRIALFEDCIDAWIQIACPRLSIDWGEAFKKPLLTPFEAEIALG 369
Query: 1166 DLPGWWERKLVCNDSSECAKKEGCCGNQNVQKGVDYPMDYYAQDGGEWNSSYSKKSAR 1339
DLPGWWE+ C C+ GC G DYPMDYYAQDGGEWNS+Y KK+ R
Sbjct: 370 DLPGWWEKSAGCGSDDLCS---GC------TNGGDYPMDYYAQDGGEWNSAYVKKATR 418
>ref|XP_002265458.1| PREDICTED: diphthamide biosynthesis protein 1-like [Vitis vinifera]
Length = 461
Score = 583 bits (1502), Expect = e-164
Identities = 282/441 (63%), Positives = 340/441 (77%), Gaps = 10/441 (2%)
Frame = +2
Query: 95 SKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPEG 274
S PKRFV++QIPDSI+N+AALNAA++LLP+NYNFE+HK +WR+ S+ A RVALQFPEG
Sbjct: 26 SSSGPKRFVKNQIPDSIINNAALNAAIALLPSNYNFEVHKCVWRILSSRAKRVALQFPEG 85
Query: 275 LLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDS 454
LLMYSL +SDI T++A HC +LGDVTYGACCVDDLSA AL+ LL+H+GHSCLVPID+
Sbjct: 86 LLMYSLHLSDIFTSYAGADHCLVLGDVTYGACCVDDLSAAALEADLLIHYGHSCLVPIDA 145
Query: 455 TTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGFR 634
T++P LY+FVEIAI+ L+ +K N P V+AGTIQFS+AIRA KPELE GFR
Sbjct: 146 TSIPCLYVFVEIAIDVDLLIGTVKLNLPP--AGQIVLAGTIQFSSAIRAAKPELEKAGFR 203
Query: 635 VLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRFD 814
VLIPQSKPLSAGEVLGCTAP + D V +FVADGRFHLEAFMIANP IK +R+D
Sbjct: 204 VLIPQSKPLSAGEVLGCTAPKISKTSFISDDVVAVFVADGRFHLEAFMIANPAIKAYRYD 263
Query: 815 PYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKMKDKG 994
PY+G+L LEEYDHKGMK++R+NAI +A+ K WGIVLGTLGRQGNP +LN L+ +M++KG
Sbjct: 264 PYIGRLLLEEYDHKGMKDSRKNAILKAREGKNWGIVLGTLGRQGNPRILNMLQDRMREKG 323
Query: 995 LDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGDLP 1174
+ VVL+SE+SP ++ +F D+VDAW+QIACPRLSIDWGDAF KPLL FEAEIALG +P
Sbjct: 324 FSWTVVLMSEISPARVLLFGDSVDAWIQIACPRLSIDWGDAFGKPLLNPFEAEIALGFIP 383
Query: 1175 GWWERKLVCNDSSECAKKEGCCGNQNVQKGV----------DYPMDYYAQDGGEWNSSYS 1324
GWWE+ + N S+C K CC N N GV DYPMDYYAQDGGEWNSSY+
Sbjct: 384 GWWEKASIAN--SDCIKNGLCC-NDNCDNGVCAKDKVDVSQDYPMDYYAQDGGEWNSSYA 440
Query: 1325 KKSARDRVKKDQCCNGSSVKS 1387
KKS R + C G+ S
Sbjct: 441 KKSTRPSRRNVSSCTGNGAIS 461
>ref|XP_002864766.1| diphthamide synthesis DPH2 family protein [Arabidopsis lyrata subsp.
lyrata] gi|297310601|gb|EFH41025.1| diphthamide synthesis
DPH2 family protein [Arabidopsis lyrata subsp. lyrata]
Length = 453
Score = 581 bits (1498), Expect = e-163
Identities = 288/445 (64%), Positives = 346/445 (77%), Gaps = 15/445 (3%)
Frame = +2
Query: 50 ETSAANNQLVEQPPPSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRV 229
E S NN +P K PKRF+++QIP+SIL DA+LNAA+S+LP+ Y FE+HK +WR+
Sbjct: 2 ELSDLNNGKTNKP---KQPPKRFIKNQIPESILGDASLNAAISILPSIYQFEVHKCVWRI 58
Query: 230 RSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVH 409
+S+NA R+ALQ PEGLLMY+L +SDI T+FA +HCF+LGDVTYGACCVDD SA AL
Sbjct: 59 KSSNAKRIALQLPEGLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSACALGAD 118
Query: 410 LLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSN 589
LL+H+GHSCLVPIDST +P LY+FVEI I+ + LL+ + N + D N ++AGTIQF++
Sbjct: 119 LLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNTIHMNLASDA-KNIILAGTIQFTS 177
Query: 590 AIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLE 769
AIRAVKPELE GF V+IPQSKPLSAGEVLGCTAP +K + GKD V++FVADGRFHLE
Sbjct: 178 AIRAVKPELEKQGFNVMIPQSKPLSAGEVLGCTAPKIKTVE-DGKDQVLVFVADGRFHLE 236
Query: 770 AFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGN 949
AFMIANP IK FR+DPYLGKLFLEEYDHKGM+E RR AI RA+ AKTWGIVLGTLGRQGN
Sbjct: 237 AFMIANPKIKAFRYDPYLGKLFLEEYDHKGMRETRRRAIARARDAKTWGIVLGTLGRQGN 296
Query: 950 PVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKP 1129
P++L RLE KM +KG+D VVL+SELSP ++ +F D+VDAWVQIACPRLSIDWG+AF KP
Sbjct: 297 PMILERLEKKMVEKGIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKP 356
Query: 1130 LLTTFEAEIALGDLPGWWER--KLVCNDSSECAKKEG----CCGNQNVQKGV-------- 1267
LLTTFEAEIALG + GWWE SS C K++ C N+ V+
Sbjct: 357 LLTTFEAEIALGFIRGWWENGSSSRVESSSGCCKEDKERSCACKNEKVEDDKKDNDGALD 416
Query: 1268 -DYPMDYYAQDGGEWNSSYSKKSAR 1339
DYPMDYYAQ+GGEWNSSY KKS+R
Sbjct: 417 GDYPMDYYAQEGGEWNSSYLKKSSR 441
>ref|XP_006493302.1| PREDICTED: diphthamide biosynthesis protein 1-like [Citrus sinensis]
Length = 436
Score = 580 bits (1496), Expect = e-163
Identities = 279/416 (67%), Positives = 343/416 (82%), Gaps = 4/416 (0%)
Frame = +2
Query: 95 SKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPEG 274
+KP+PKRFV++QIP+SILN+A+LN+A++LLP NY+FEIHK +WR+RS A RVALQ PEG
Sbjct: 11 TKPKPKRFVKNQIPESILNNASLNSAIALLPHNYSFEIHKCVWRIRSTRAKRVALQLPEG 70
Query: 275 LLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDS 454
LLMYSL++SDI T+FA VTHCFILGDVTYGACCVDDLSA AL+ LL+H+GHSCLVP+D
Sbjct: 71 LLMYSLILSDIFTSFADVTHCFILGDVTYGACCVDDLSASALNADLLIHYGHSCLVPVDF 130
Query: 455 TTVPVLYIFVEIAINTRKLLHELKYNFS-PDKISNFVMAGTIQFSNAIRAVKPELENLGF 631
T +P LY+FVEI I+ +L+ +K N+S P K+ ++AGTIQF++AIRA KPELE GF
Sbjct: 131 TRIPCLYVFVEIKIDVNRLIDTIKVNYSDPGKL---ILAGTIQFASAIRAAKPELEKQGF 187
Query: 632 RVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRF 811
+V+IPQSKPLSAGEVLGCTAP + ++ D ++F+ADGRFHLEAFMI+NP IKTFR+
Sbjct: 188 KVMIPQSKPLSAGEVLGCTAPKIPARE---SDFNLVFIADGRFHLEAFMISNPGIKTFRY 244
Query: 812 DPYLGKLFLEEYDHKGMKENRRNAIERA-KGAKTWGIVLGTLGRQGNPVVLNRLEGKMKD 988
DPYLGKLFLEEYD+KGM+E R+ AIE+A K A+TWGIVLGTLGRQGNP +L RL+ +M+
Sbjct: 245 DPYLGKLFLEEYDNKGMRETRKRAIEKAMKEARTWGIVLGTLGRQGNPRILERLQKRMEK 304
Query: 989 KGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGD 1168
KG DY+V+++SE+SP ++ +F D+VDAW+QIACPRLSIDWGDAF KPLLT FEAEIALG
Sbjct: 305 KGFDYVVIMMSEISPARVALFEDSVDAWIQIACPRLSIDWGDAFTKPLLTPFEAEIALGV 364
Query: 1169 LPGWWERKLVCNDSSECAKKEGCCGNQ--NVQKGVDYPMDYYAQDGGEWNSSYSKK 1330
+PGWWER E +K G CGN+ N DYPMDYYAQDGGEWNSSY KK
Sbjct: 365 IPGWWERDKEREREREESKSCGGCGNEDKNCDGDGDYPMDYYAQDGGEWNSSYVKK 420
>ref|XP_006394442.1| hypothetical protein EUTSA_v10004160mg [Eutrema salsugineum]
gi|557091081|gb|ESQ31728.1| hypothetical protein
EUTSA_v10004160mg [Eutrema salsugineum]
Length = 464
Score = 580 bits (1495), Expect = e-163
Identities = 289/441 (65%), Positives = 338/441 (76%), Gaps = 25/441 (5%)
Frame = +2
Query: 92 PSKPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPE 271
P +P PKRFV++QIP+SIL DA+LNAA+SLLP+ Y FE+HK +WR++S+NA R+ALQ PE
Sbjct: 14 PKQP-PKRFVKNQIPESILGDASLNAAISLLPSIYQFEVHKCVWRIKSSNAKRIALQLPE 72
Query: 272 GLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPID 451
GLLMY+L +SDI T+FA +HCF+LGDVTYGACCVDD SA AL LL+H+GHSCLVPID
Sbjct: 73 GLLMYALTLSDIFTSFAGASHCFVLGDVTYGACCVDDFSASALGADLLIHYGHSCLVPID 132
Query: 452 STTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGF 631
ST +P LY+FVEI I+ + LL+ + N S D N ++AGTIQF++AIRAVKPELE GF
Sbjct: 133 STKIPCLYVFVEIQIDVKCLLNTIHLNLSADS-KNIILAGTIQFTSAIRAVKPELEKQGF 191
Query: 632 RVLIPQSKPLSAGEVLGCTAPSVKLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRF 811
VLIPQSKPLSAGEVLGCTAP + D KD V++FVADGRFHLEAFMIANP IK FR+
Sbjct: 192 NVLIPQSKPLSAGEVLGCTAPKITRVD-EDKDAVLVFVADGRFHLEAFMIANPKIKAFRY 250
Query: 812 DPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGIVLGTLGRQGNPVVLNRLEGKMKDK 991
DPYLGK+FLEEYDHKGM+E RR AI RAK AKTWGIVLGTLGRQGNP +L RLE KM +K
Sbjct: 251 DPYLGKMFLEEYDHKGMRETRRRAISRAKDAKTWGIVLGTLGRQGNPKILERLEKKMMEK 310
Query: 992 GLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGDL 1171
G+D VVL+SELSP ++ +F D+VDAWVQIACPRLSIDWG+AF KPLLTTFEAEIALG +
Sbjct: 311 GIDSTVVLMSELSPTRVALFEDSVDAWVQIACPRLSIDWGEAFLKPLLTTFEAEIALGLI 370
Query: 1172 PGWWERKLVCNDSSECAKKEGC----------CGNQNVQKG---------------VDYP 1276
GWWE SS GC CGN V+ G DYP
Sbjct: 371 RGWWENGSGSVSSSRVDSPSGCCKEDKEASCACGNGKVEDGNKKDDDDDDDDGVMDGDYP 430
Query: 1277 MDYYAQDGGEWNSSYSKKSAR 1339
MDYYAQ+GGEWNSSY KKS+R
Sbjct: 431 MDYYAQEGGEWNSSYLKKSSR 451
>ref|XP_006592079.1| PREDICTED: diphthamide biosynthesis protein 1-like [Glycine max]
Length = 462
Score = 580 bits (1494), Expect = e-163
Identities = 285/443 (64%), Positives = 342/443 (77%), Gaps = 13/443 (2%)
Frame = +2
Query: 50 ETSAANNQLV---EQPPPS--KPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHK 214
E A N LV EQP + KP+PKRFV++QIPDSILN+ L+AA+S+LP+NYNFE+HK
Sbjct: 2 EKLEAENVLVLPSEQPNNNRPKPKPKRFVKNQIPDSILNNPLLSAAISVLPSNYNFEVHK 61
Query: 215 IIWRVRSANATRVALQFPEGLLMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSAR 394
+WRV S A RVALQFPEGLLMYSL +SDI TTFA VTHCF+L DVTYGACCVDDL+A
Sbjct: 62 CVWRVLSTGAKRVALQFPEGLLMYSLPLSDIFTTFAGVTHCFVLADVTYGACCVDDLAAS 121
Query: 395 ALDVHLLVHFGHSCLVPIDSTTVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGT 574
AL LL+H+GHSCLVPID+TT+P LY+FV+I I+ + L N + V+AGT
Sbjct: 122 ALGADLLIHYGHSCLVPIDATTIPCLYVFVDIKIDVPHFVDTLSLNLLA-QTKTLVVAGT 180
Query: 575 IQFSNAIRAVKPELENLGFRVLIPQSKPLSAGEVLGCTAPSVKLQDCAGK-----DDVII 739
IQF++AIRA KP+LE LGFRVLIPQSKPLSAGEVLGCTAP V G + V++
Sbjct: 181 IQFASAIRAAKPQLEELGFRVLIPQSKPLSAGEVLGCTAPKVSSSKLLGGGGDIGECVLV 240
Query: 740 FVADGRFHLEAFMIANPDIKTFRFDPYLGKLFLEEYDHKGMKENRRNAIERAKGAKTWGI 919
FVADGRFHLEAFMIANP I+ FR+DPY+GKLFLEEYDH GMK +R+NAI +A+ A++WG+
Sbjct: 241 FVADGRFHLEAFMIANPGIRAFRYDPYMGKLFLEEYDHLGMKRSRKNAIFKAREARSWGL 300
Query: 920 VLGTLGRQGNPVVLNRLEGKMKDKGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLS 1099
VLGTLGRQGNP +L RLE M+++GLDY VVL+SE+SP +I +F D++DAW+QIACPRLS
Sbjct: 301 VLGTLGRQGNPRILERLERMMRERGLDYTVVLMSEMSPTRIALFEDSLDAWIQIACPRLS 360
Query: 1100 IDWGDAFKKPLLTTFEAEIALGDLPGWWERKLVCNDSSECAKKEGCC---GNQNVQKGVD 1270
IDWG+AF KP+LT FEAE+ALG +PGWWE+ VC + C K G C G D
Sbjct: 361 IDWGEAFVKPVLTPFEAEVALGVIPGWWEKNEVCEEGEGCCNKSGSCCEDAKGGEDFGGD 420
Query: 1271 YPMDYYAQDGGEWNSSYSKKSAR 1339
YPMDYYAQDGGEWNSSY KKS R
Sbjct: 421 YPMDYYAQDGGEWNSSYVKKSTR 443
>ref|XP_003533490.1| PREDICTED: diphthamide biosynthesis protein 1-like [Glycine max]
Length = 467
Score = 576 bits (1485), Expect = e-162
Identities = 282/425 (66%), Positives = 339/425 (79%), Gaps = 6/425 (1%)
Frame = +2
Query: 98 KPRPKRFVRSQIPDSILNDAALNAAVSLLPANYNFEIHKIIWRVRSANATRVALQFPEGL 277
KP+PKRFV++QIP+SILND LNAA+S+LP+NYNFE+HK +WRV S+ A RVALQFPEGL
Sbjct: 31 KPKPKRFVKNQIPESILNDPVLNAAISVLPSNYNFEVHKCVWRVLSSGAKRVALQFPEGL 90
Query: 278 LMYSLVISDILTTFAAVTHCFILGDVTYGACCVDDLSARALDVHLLVHFGHSCLVPIDST 457
LMYSL +SDILT+FAAVTHC++LGDVTYGACCVDDL+A AL LL+H+GHSCLVPIDST
Sbjct: 91 LMYSLPLSDILTSFAAVTHCYVLGDVTYGACCVDDLAASALGADLLIHYGHSCLVPIDST 150
Query: 458 TVPVLYIFVEIAINTRKLLHELKYNFSPDKISNFVMAGTIQFSNAIRAVKPELENLGFRV 637
T+P LYIFV+I I+ + L N K V+AGTIQF++AIR KP+LE LGFRV
Sbjct: 151 TIPCLYIFVDIKIDVPHFVDTLILNL--HKAKTLVIAGTIQFASAIRTAKPQLEELGFRV 208
Query: 638 LIPQSKPLSAGEVLGCTAPSV--KLQDCAGKDDVIIFVADGRFHLEAFMIANPDIKTFRF 811
LIPQSKPLSAGEVLGCTAP V KL G+ V++FVADGRFHLEAFMIANP I+ FR+
Sbjct: 209 LIPQSKPLSAGEVLGCTAPKVSSKLLGDDGESSVLVFVADGRFHLEAFMIANPGIRAFRY 268
Query: 812 DPYLGKLFLEEYDHKGMKENRRNAIERAK-GAKTWGIVLGTLGRQGNPVVLNRLEGKMKD 988
DPY+GKLFLEEYDH GMK +R+NAI +A+ A++WG+VLGTLGRQGNP +L RLE M+D
Sbjct: 269 DPYMGKLFLEEYDHLGMKRSRKNAIFKAREEARSWGLVLGTLGRQGNPRILERLEKMMRD 328
Query: 989 KGLDYMVVLISELSPKKIEMFSDAVDAWVQIACPRLSIDWGDAFKKPLLTTFEAEIALGD 1168
+G DY VVL+SE+SP +I +F D++DAW+QIACPRLSIDWG+AF KP+LT FEA+IALG
Sbjct: 329 RGFDYTVVLMSEMSPARIALFEDSIDAWIQIACPRLSIDWGEAFVKPVLTPFEADIALGV 388
Query: 1169 LPGWWERKLVCNDSSECAKKEGCCGNQ---NVQKGVDYPMDYYAQDGGEWNSSYSKKSAR 1339
+PGWWE+ N+ C K CCG+ G DYPMDYYAQDGGEWNSSY KS R
Sbjct: 389 IPGWWEKN---NEVVCCNKSGSCCGDAKGGGEDFGGDYPMDYYAQDGGEWNSSYVIKSTR 445
Query: 1340 DRVKK 1354
++
Sbjct: 446 PPARR 450