BLASTX nr result
ID: Mentha26_contig00035901
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha26_contig00035901
(314 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EPS70903.1| hypothetical protein M569_03854, partial [Genlise... 194 1e-47
ref|XP_004135530.1| PREDICTED: protein translocase subunit SECA1... 193 2e-47
ref|NP_192089.1| protein translocase subunit secA [Arabidopsis ... 192 4e-47
ref|XP_002872876.1| protein translocase subunit secA chloroplast... 192 4e-47
ref|XP_006349142.1| PREDICTED: protein translocase subunit SECA1... 191 9e-47
ref|XP_006492424.1| PREDICTED: protein translocase subunit SECA1... 191 1e-46
ref|XP_002320935.1| preprotein translocase secA subunit [Populus... 191 1e-46
ref|XP_006589081.1| PREDICTED: protein translocase subunit SecA,... 189 4e-46
ref|XP_006589079.1| PREDICTED: protein translocase subunit SecA,... 189 4e-46
ref|XP_006589080.1| PREDICTED: protein translocase subunit SecA,... 189 4e-46
gb|AAD22642.1|AC007138_6 putative SecA-type chloroplast protein ... 187 1e-45
ref|XP_004968649.1| PREDICTED: LOW QUALITY PROTEIN: protein tran... 187 1e-45
ref|XP_002457762.1| hypothetical protein SORBIDRAFT_03g013090 [S... 186 2e-45
ref|XP_004495859.1| PREDICTED: protein translocase subunit SecA,... 186 4e-45
ref|XP_004495858.1| PREDICTED: protein translocase subunit SecA,... 186 4e-45
gb|AAB09598.1| cp-SecA [Zea mays] 185 6e-45
dbj|BAD44978.1| putative SecA [Oryza sativa Japonica Group] 185 6e-45
gb|EAZ11664.1| hypothetical protein OsJ_01526 [Oryza sativa Japo... 185 6e-45
gb|EAY73759.1| hypothetical protein OsI_01632 [Oryza sativa Indi... 185 6e-45
ref|XP_003567619.1| PREDICTED: protein translocase subunit SecA,... 184 8e-45
>gb|EPS70903.1| hypothetical protein M569_03854, partial [Genlisea aurea]
Length = 753
Score = 194 bits (492), Expect = 1e-47
Identities = 94/104 (90%), Positives = 101/104 (97%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAMS ELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK
Sbjct: 20 NSELGFDYLRDNLAMSTSELVLRSFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 79
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAK+A+AFERDIHYTVDEKQK+VL++EQGYADTEEIL+VKDLYD
Sbjct: 80 AAKVAAAFERDIHYTVDEKQKTVLLSEQGYADTEEILDVKDLYD 123
>ref|XP_004135530.1| PREDICTED: protein translocase subunit SECA1, chloroplastic-like
[Cucumis sativus]
Length = 1025
Score = 193 bits (490), Expect = 2e-47
Identities = 95/104 (91%), Positives = 100/104 (96%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVEELVLR F+YCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK
Sbjct: 250 NSELGFDYLRDNLATSVEELVLRDFSYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 309
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAK+ASAFERDIHYTVDEKQK+VL+TEQGY D EEILNVKDLYD
Sbjct: 310 AAKLASAFERDIHYTVDEKQKTVLLTEQGYEDAEEILNVKDLYD 353
>ref|NP_192089.1| protein translocase subunit secA [Arabidopsis thaliana]
gi|322510072|sp|Q9SYI0.2|SECA1_ARATH RecName:
Full=Protein translocase subunit SECA1, chloroplastic;
Short=AtcpSecA; AltName: Full=Protein ALBINO OR GLASSY
YELLOW 1; Flags: Precursor gi|332656678|gb|AEE82078.1|
protein translocase subunit secA [Arabidopsis thaliana]
Length = 1022
Score = 192 bits (488), Expect = 4e-47
Identities = 95/104 (91%), Positives = 100/104 (96%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVEELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSD+YYK
Sbjct: 253 NSELGFDYLRDNLATSVEELVLRDFNYCVIDEVDSILIDEARTPLIISGPAEKPSDQYYK 312
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIASAFERDIHYTVDEKQK+VL+TEQGY D EEIL+VKDLYD
Sbjct: 313 AAKIASAFERDIHYTVDEKQKTVLLTEQGYEDAEEILDVKDLYD 356
>ref|XP_002872876.1| protein translocase subunit secA chloroplast precursor [Arabidopsis
lyrata subsp. lyrata] gi|297318713|gb|EFH49135.1|
protein translocase subunit secA chloroplast precursor
[Arabidopsis lyrata subsp. lyrata]
Length = 1022
Score = 192 bits (488), Expect = 4e-47
Identities = 95/104 (91%), Positives = 100/104 (96%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVEELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSD+YYK
Sbjct: 253 NSELGFDYLRDNLATSVEELVLRDFNYCVIDEVDSILIDEARTPLIISGPAEKPSDQYYK 312
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIASAFERDIHYTVDEKQK+VL+TEQGY D EEIL+VKDLYD
Sbjct: 313 AAKIASAFERDIHYTVDEKQKTVLLTEQGYEDAEEILDVKDLYD 356
>ref|XP_006349142.1| PREDICTED: protein translocase subunit SECA1, chloroplastic-like
[Solanum tuberosum]
Length = 1020
Score = 191 bits (485), Expect = 9e-47
Identities = 92/104 (88%), Positives = 101/104 (97%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SV+ELV+R FNYCVIDEVDSILIDEARTPLIISGPAEKPSD+YYK
Sbjct: 252 NSELGFDYLRDNLATSVDELVVRNFNYCVIDEVDSILIDEARTPLIISGPAEKPSDQYYK 311
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAK+A+AFERDIHYTVDEKQK+VL+TEQGYAD EEIL+VKDLYD
Sbjct: 312 AAKVAAAFERDIHYTVDEKQKNVLLTEQGYADAEEILDVKDLYD 355
>ref|XP_006492424.1| PREDICTED: protein translocase subunit SECA1, chloroplastic-like
[Citrus sinensis]
Length = 1017
Score = 191 bits (484), Expect = 1e-46
Identities = 93/104 (89%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SV+ELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSD+YYK
Sbjct: 243 NSELGFDYLRDNLATSVDELVLRNFNYCVIDEVDSILIDEARTPLIISGPAEKPSDKYYK 302
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIAS FERDIHYTVDEKQK+VL+TEQGY D EEIL+VKDLYD
Sbjct: 303 AAKIASVFERDIHYTVDEKQKTVLLTEQGYEDAEEILDVKDLYD 346
>ref|XP_002320935.1| preprotein translocase secA subunit [Populus trichocarpa]
gi|222861708|gb|EEE99250.1| preprotein translocase secA
subunit [Populus trichocarpa]
Length = 963
Score = 191 bits (484), Expect = 1e-46
Identities = 96/107 (89%), Positives = 102/107 (95%), Gaps = 3/107 (2%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAM---SVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDR 171
NSE+GFDYLRDNLAM +VEELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSDR
Sbjct: 186 NSELGFDYLRDNLAMEIQTVEELVLRDFNYCVIDEVDSILIDEARTPLIISGPAEKPSDR 245
Query: 172 YYKAAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
YYKAAKIA+AFERDIHYTVDEKQK+VL+TEQGY DTEEIL+VKDLYD
Sbjct: 246 YYKAAKIATAFERDIHYTVDEKQKTVLLTEQGYGDTEEILDVKDLYD 292
>ref|XP_006589081.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
isoform X3 [Glycine max]
Length = 860
Score = 189 bits (479), Expect = 4e-46
Identities = 92/104 (88%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVE+LV+R FNYC+IDEVDSILIDEARTPLIISGPAEKPSDRYYK
Sbjct: 88 NSELGFDYLRDNLATSVEDLVIRGFNYCIIDEVDSILIDEARTPLIISGPAEKPSDRYYK 147
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQKSVL++EQGY D+EEIL VKDLYD
Sbjct: 148 AAKIAEAFERDIHYTVDEKQKSVLLSEQGYEDSEEILAVKDLYD 191
>ref|XP_006589079.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
isoform X1 [Glycine max]
Length = 1016
Score = 189 bits (479), Expect = 4e-46
Identities = 92/104 (88%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVE+LV+R FNYC+IDEVDSILIDEARTPLIISGPAEKPSDRYYK
Sbjct: 244 NSELGFDYLRDNLATSVEDLVIRGFNYCIIDEVDSILIDEARTPLIISGPAEKPSDRYYK 303
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQKSVL++EQGY D+EEIL VKDLYD
Sbjct: 304 AAKIAEAFERDIHYTVDEKQKSVLLSEQGYEDSEEILAVKDLYD 347
>ref|XP_006589080.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
isoform X2 [Glycine max]
Length = 1014
Score = 189 bits (479), Expect = 4e-46
Identities = 92/104 (88%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVE+LV+R FNYC+IDEVDSILIDEARTPLIISGPAEKPSDRYYK
Sbjct: 244 NSELGFDYLRDNLATSVEDLVIRGFNYCIIDEVDSILIDEARTPLIISGPAEKPSDRYYK 303
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQKSVL++EQGY D+EEIL VKDLYD
Sbjct: 304 AAKIAEAFERDIHYTVDEKQKSVLLSEQGYEDSEEILAVKDLYD 347
>gb|AAD22642.1|AC007138_6 putative SecA-type chloroplast protein transport factor
[Arabidopsis thaliana] gi|7268223|emb|CAB77750.1|
putative SecA-type chloroplast protein transport factor
[Arabidopsis thaliana]
Length = 1021
Score = 187 bits (476), Expect = 1e-45
Identities = 95/105 (90%), Positives = 100/105 (95%), Gaps = 1/105 (0%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAM-SVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYY 177
NSE+GFDYLRDNLA SVEELVLR FNYCVIDEVDSILIDEARTPLIISGPAEKPSD+YY
Sbjct: 253 NSELGFDYLRDNLATESVEELVLRDFNYCVIDEVDSILIDEARTPLIISGPAEKPSDQYY 312
Query: 178 KAAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
KAAKIASAFERDIHYTVDEKQK+VL+TEQGY D EEIL+VKDLYD
Sbjct: 313 KAAKIASAFERDIHYTVDEKQKTVLLTEQGYEDAEEILDVKDLYD 357
>ref|XP_004968649.1| PREDICTED: LOW QUALITY PROTEIN: protein translocase subunit SECA1,
chloroplastic-like [Setaria italica]
Length = 1016
Score = 187 bits (475), Expect = 1e-45
Identities = 90/104 (86%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+++ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 248 NSELGFDYLRDNLAMTIDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 307
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQ++VL+TEQGYAD EEIL++ DLYD
Sbjct: 308 AAKIAEAFERDIHYTVDEKQRNVLLTEQGYADAEEILDINDLYD 351
>ref|XP_002457762.1| hypothetical protein SORBIDRAFT_03g013090 [Sorghum bicolor]
gi|241929737|gb|EES02882.1| hypothetical protein
SORBIDRAFT_03g013090 [Sorghum bicolor]
Length = 1008
Score = 186 bits (473), Expect = 2e-45
Identities = 90/104 (86%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 248 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 307
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQ++VL+TE+GYAD EEIL++ DLYD
Sbjct: 308 AAKIADAFERDIHYTVDEKQRNVLLTEEGYADAEEILDINDLYD 351
>ref|XP_004495859.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
isoform X3 [Cicer arietinum]
Length = 857
Score = 186 bits (471), Expect = 4e-45
Identities = 91/104 (87%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVEELV+R F+YCVIDEVDSILIDEARTPLIISGPA+KPSDRYYK
Sbjct: 88 NSELGFDYLRDNLATSVEELVIRGFSYCVIDEVDSILIDEARTPLIISGPADKPSDRYYK 147
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQK+VL++EQGY D EEIL VKDLYD
Sbjct: 148 AAKIAQAFERDIHYTVDEKQKTVLLSEQGYEDAEEILAVKDLYD 191
>ref|XP_004495858.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
isoform X2 [Cicer arietinum]
Length = 972
Score = 186 bits (471), Expect = 4e-45
Identities = 91/104 (87%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLA SVEELV+R F+YCVIDEVDSILIDEARTPLIISGPA+KPSDRYYK
Sbjct: 241 NSELGFDYLRDNLATSVEELVIRGFSYCVIDEVDSILIDEARTPLIISGPADKPSDRYYK 300
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQK+VL++EQGY D EEIL VKDLYD
Sbjct: 301 AAKIAQAFERDIHYTVDEKQKTVLLSEQGYEDAEEILAVKDLYD 344
>gb|AAB09598.1| cp-SecA [Zea mays]
Length = 291
Score = 185 bits (469), Expect = 6e-45
Identities = 89/104 (85%), Positives = 99/104 (95%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AE+PSDRYYK
Sbjct: 147 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAERPSDRYYK 206
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFERDIHYTVDEKQ++VL+TE+GYAD EEIL++ DLYD
Sbjct: 207 AAKIAEAFERDIHYTVDEKQRNVLLTEEGYADAEEILDIDDLYD 250
>dbj|BAD44978.1| putative SecA [Oryza sativa Japonica Group]
Length = 1021
Score = 185 bits (469), Expect = 6e-45
Identities = 89/104 (85%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 255 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 314
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA FERDIHYTVDEKQ++VL+TE+GYAD EEIL++ DLYD
Sbjct: 315 AAKIAEVFERDIHYTVDEKQRNVLLTEEGYADAEEILDINDLYD 358
>gb|EAZ11664.1| hypothetical protein OsJ_01526 [Oryza sativa Japonica Group]
Length = 1004
Score = 185 bits (469), Expect = 6e-45
Identities = 89/104 (85%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 175 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 234
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA FERDIHYTVDEKQ++VL+TE+GYAD EEIL++ DLYD
Sbjct: 235 AAKIAEVFERDIHYTVDEKQRNVLLTEEGYADAEEILDINDLYD 278
>gb|EAY73759.1| hypothetical protein OsI_01632 [Oryza sativa Indica Group]
Length = 978
Score = 185 bits (469), Expect = 6e-45
Identities = 89/104 (85%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 149 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 208
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA FERDIHYTVDEKQ++VL+TE+GYAD EEIL++ DLYD
Sbjct: 209 AAKIAEVFERDIHYTVDEKQRNVLLTEEGYADAEEILDINDLYD 252
>ref|XP_003567619.1| PREDICTED: protein translocase subunit SecA, chloroplastic-like
[Brachypodium distachyon]
Length = 935
Score = 184 bits (468), Expect = 8e-45
Identities = 89/104 (85%), Positives = 98/104 (94%)
Frame = +1
Query: 1 NSEMGFDYLRDNLAMSVEELVLRRFNYCVIDEVDSILIDEARTPLIISGPAEKPSDRYYK 180
NSE+GFDYLRDNLAM+V+ELVLR FNYCVIDEVDSILIDEARTPLIISG AEKPSDRYYK
Sbjct: 175 NSELGFDYLRDNLAMTVDELVLRNFNYCVIDEVDSILIDEARTPLIISGLAEKPSDRYYK 234
Query: 181 AAKIASAFERDIHYTVDEKQKSVLITEQGYADTEEILNVKDLYD 312
AAKIA AFE+DIHYT DEKQ++VL+TEQGYAD EEIL++ DLYD
Sbjct: 235 AAKIAEAFEQDIHYTADEKQRNVLLTEQGYADAEEILDINDLYD 278