BLASTX nr result
ID: Mentha26_contig00024741
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha26_contig00024741
(348 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004235076.1| PREDICTED: vacuolar amino acid transporter 1... 165 5e-39
ref|XP_006355151.1| PREDICTED: vacuolar amino acid transporter 1... 163 3e-38
ref|XP_007145646.1| hypothetical protein PHAVU_007G256500g [Phas... 160 2e-37
ref|XP_004513746.1| PREDICTED: vacuolar amino acid transporter 1... 160 2e-37
ref|XP_006575238.1| PREDICTED: vacuolar amino acid transporter 1... 160 2e-37
ref|XP_006588913.1| PREDICTED: vacuolar amino acid transporter 1... 158 6e-37
gb|EXB38301.1| hypothetical protein L484_013934 [Morus notabilis] 157 1e-36
ref|XP_002266914.2| PREDICTED: vacuolar amino acid transporter 1... 157 1e-36
emb|CBI30124.3| unnamed protein product [Vitis vinifera] 157 1e-36
ref|XP_006421100.1| hypothetical protein CICLE_v10004676mg [Citr... 155 7e-36
ref|XP_006411385.1| hypothetical protein EUTSA_v10016504mg [Eutr... 154 1e-35
ref|XP_006477498.1| PREDICTED: vacuolar amino acid transporter 1... 154 2e-35
ref|NP_030664.1| transmembrane amino acid transporter family pro... 150 1e-34
gb|AAN31931.1| unknown protein [Arabidopsis thaliana] 150 1e-34
ref|XP_006573773.1| PREDICTED: vesicular inhibitory amino acid t... 150 2e-34
ref|XP_006573771.1| PREDICTED: vesicular inhibitory amino acid t... 150 2e-34
ref|XP_002881760.1| amino acid transporter family protein [Arabi... 150 2e-34
ref|XP_006293964.1| hypothetical protein CARUB_v10022954mg [Caps... 150 2e-34
ref|XP_004305687.1| PREDICTED: vacuolar amino acid transporter 1... 149 5e-34
gb|EPS65847.1| amino acid transporter family protein, partial [G... 147 1e-33
>ref|XP_004235076.1| PREDICTED: vacuolar amino acid transporter 1-like [Solanum
lycopersicum]
Length = 525
Score = 165 bits (418), Expect = 5e-39
Identities = 80/110 (72%), Positives = 96/110 (87%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPL R IEELLP RIS S W ++LLRTALV STV AF++PFFGLVMALIGSL SILV+
Sbjct: 416 MNPLGRCIEELLPERISSSLWCFLLLRTALVFSTVCVAFLLPFFGLVMALIGSLFSILVS 475
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
+I+PALC+LKI+GKKAT+TQ + S+S+I G++CAALGTYSSLSGIAN+Y
Sbjct: 476 IIIPALCYLKIIGKKATRTQRVSSISVIALGIVCAALGTYSSLSGIANKY 525
>ref|XP_006355151.1| PREDICTED: vacuolar amino acid transporter 1-like [Solanum
tuberosum]
Length = 523
Score = 163 bits (412), Expect = 3e-38
Identities = 81/110 (73%), Positives = 95/110 (86%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPL R IEELLP RIS S W +ILLRT LV STV AF++PFFGLVMALIGSLLSILV+
Sbjct: 414 MNPLGRCIEELLPERISSSLWCFILLRTVLVFSTVCVAFLLPFFGLVMALIGSLLSILVS 473
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VIMPALC+LKI+GKKAT+TQ + S+++I G++ AALGTYSSLSGIAN+Y
Sbjct: 474 VIMPALCYLKIIGKKATRTQRVFSITVIAVGIVSAALGTYSSLSGIANKY 523
>ref|XP_007145646.1| hypothetical protein PHAVU_007G256500g [Phaseolus vulgaris]
gi|561018836|gb|ESW17640.1| hypothetical protein
PHAVU_007G256500g [Phaseolus vulgaris]
Length = 520
Score = 160 bits (405), Expect = 2e-37
Identities = 78/110 (70%), Positives = 94/110 (85%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS ++W +ILLRTALV STV AF++PFFGLVMALIGSL S+LV+
Sbjct: 411 MNPLARSLEELLPERISTTYWCFILLRTALVASTVCVAFLVPFFGLVMALIGSLFSLLVS 470
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
IMP LCFLKI+GKKATKTQ+++S++I V G+ICA LGTYSSL IA+ Y
Sbjct: 471 SIMPTLCFLKIIGKKATKTQVVLSVAITVLGIICAVLGTYSSLMSIADSY 520
>ref|XP_004513746.1| PREDICTED: vacuolar amino acid transporter 1-like [Cicer arietinum]
Length = 530
Score = 160 bits (405), Expect = 2e-37
Identities = 80/110 (72%), Positives = 93/110 (84%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS ++W +ILLRTALV+STV AF+IPFFGLVMALIGSL S+LV+
Sbjct: 421 MNPLARSLEELLPDRISSTYWCFILLRTALVISTVCAAFLIPFFGLVMALIGSLFSMLVS 480
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VIMP+LCFLKI+GKKATKTQ+ MS+ I G+IC LGTYSSL IAN Y
Sbjct: 481 VIMPSLCFLKIVGKKATKTQVTMSVIIAALGIICGLLGTYSSLLNIANSY 530
>ref|XP_006575238.1| PREDICTED: vacuolar amino acid transporter 1-like [Glycine max]
Length = 534
Score = 160 bits (404), Expect = 2e-37
Identities = 78/110 (70%), Positives = 93/110 (84%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS S+W +ILLRT LV STV AF++PFFGLVMALIGSL SILV+
Sbjct: 425 MNPLARSLEELLPDRISSSYWCFILLRTTLVASTVCVAFLVPFFGLVMALIGSLFSILVS 484
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
IMP+LCFLKI+GKKAT+TQ+++S++I FGVIC LGTYSSL IA+ Y
Sbjct: 485 AIMPSLCFLKIIGKKATRTQVVLSVAIAAFGVICGILGTYSSLLSIADSY 534
>ref|XP_006588913.1| PREDICTED: vacuolar amino acid transporter 1-like isoform X1
[Glycine max] gi|571482307|ref|XP_006588914.1|
PREDICTED: vacuolar amino acid transporter 1-like
isoform X2 [Glycine max]
Length = 548
Score = 158 bits (400), Expect = 6e-37
Identities = 78/110 (70%), Positives = 92/110 (83%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS S+W ++LLRT LV STV AF++PFFGLVMALIGSL SILV+
Sbjct: 439 MNPLARSLEELLPDRISSSYWCFMLLRTTLVASTVCVAFLVPFFGLVMALIGSLFSILVS 498
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
IMP+LCFLKI+GKKATKTQ+ +S++I FGVIC LGTYSSL IA+ Y
Sbjct: 499 AIMPSLCFLKIIGKKATKTQVALSVAIAAFGVICGILGTYSSLLSIADSY 548
>gb|EXB38301.1| hypothetical protein L484_013934 [Morus notabilis]
Length = 844
Score = 157 bits (398), Expect = 1e-36
Identities = 81/110 (73%), Positives = 95/110 (86%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP R+S+S W +ILLRTALV S+V AF++PFFGLVMALIGSLLSILVA
Sbjct: 735 MNPLARSIEELLPIRVSNSPWCFILLRTALVASSVCAAFLLPFFGLVMALIGSLLSILVA 794
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VIMPALCFLKI+GKKAT Q++ S++I+V G+I A LGTYSS+S IAN Y
Sbjct: 795 VIMPALCFLKIVGKKATTVQIVSSIAIVVLGIISAILGTYSSVSQIANSY 844
>ref|XP_002266914.2| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
Length = 537
Score = 157 bits (398), Expect = 1e-36
Identities = 78/110 (70%), Positives = 94/110 (85%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP RIS+SFW +ILLRTALV+S+V AF++PFFGLVM+LIGSLLS+LV+
Sbjct: 428 MNPLARSIEELLPVRISNSFWCFILLRTALVISSVCVAFLLPFFGLVMSLIGSLLSVLVS 487
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VI+P LC+L+I+G KATK Q+I+S + GVICA LGTYSSLS IA QY
Sbjct: 488 VIIPTLCYLRIMGNKATKAQVIVSSGVAALGVICAILGTYSSLSQIARQY 537
>emb|CBI30124.3| unnamed protein product [Vitis vinifera]
Length = 110
Score = 157 bits (398), Expect = 1e-36
Identities = 78/110 (70%), Positives = 94/110 (85%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP RIS+SFW +ILLRTALV+S+V AF++PFFGLVM+LIGSLLS+LV+
Sbjct: 1 MNPLARSIEELLPVRISNSFWCFILLRTALVISSVCVAFLLPFFGLVMSLIGSLLSVLVS 60
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VI+P LC+L+I+G KATK Q+I+S + GVICA LGTYSSLS IA QY
Sbjct: 61 VIIPTLCYLRIMGNKATKAQVIVSSGVAALGVICAILGTYSSLSQIARQY 110
>ref|XP_006421100.1| hypothetical protein CICLE_v10004676mg [Citrus clementina]
gi|557522973|gb|ESR34340.1| hypothetical protein
CICLE_v10004676mg [Citrus clementina]
Length = 551
Score = 155 bits (391), Expect = 7e-36
Identities = 77/110 (70%), Positives = 95/110 (86%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP+ IS+++W +ILLRTALV S+V AF++PFF LVMALIGSLLSILVA
Sbjct: 442 MNPLARSIEELLPAGISNNYWCFILLRTALVFSSVCAAFLLPFFSLVMALIGSLLSILVA 501
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
+IMP+LCF+KILGKKAT+TQ++ S +I V GVICA LGT+SS++ IA Y
Sbjct: 502 IIMPSLCFIKILGKKATRTQIVSSTAIAVLGVICAILGTFSSVAKIAENY 551
>ref|XP_006411385.1| hypothetical protein EUTSA_v10016504mg [Eutrema salsugineum]
gi|557112554|gb|ESQ52838.1| hypothetical protein
EUTSA_v10016504mg [Eutrema salsugineum]
Length = 528
Score = 154 bits (389), Expect = 1e-35
Identities = 75/110 (68%), Positives = 93/110 (84%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP RIS++ W ++LLRTALV S+V AF+IPFFGLVMALIGSLLSILVA
Sbjct: 419 MNPLARSIEELLPERISENIWCFLLLRTALVASSVCSAFLIPFFGLVMALIGSLLSILVA 478
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
++MPALCF+KI+G KAT+TQMI+S +I+ G++ ALGTYSS++ I Y
Sbjct: 479 IVMPALCFIKIMGNKATRTQMILSSTIVAIGLVSGALGTYSSVAKIIRNY 528
>ref|XP_006477498.1| PREDICTED: vacuolar amino acid transporter 1-like isoform X1
[Citrus sinensis]
Length = 551
Score = 154 bits (388), Expect = 2e-35
Identities = 76/110 (69%), Positives = 95/110 (86%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP+ IS+++W +ILLRTALV S+V AF++PFF LVMALIGSLLSILVA
Sbjct: 442 MNPLARSIEELLPAGISNNYWCFILLRTALVFSSVCAAFLLPFFSLVMALIGSLLSILVA 501
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
+IMP+LCF+KILG+KAT+TQ++ S +I V GVICA LGT+SS++ IA Y
Sbjct: 502 IIMPSLCFIKILGRKATRTQIVSSTAIAVLGVICAILGTFSSVAKIAENY 551
>ref|NP_030664.1| transmembrane amino acid transporter family protein [Arabidopsis
thaliana] gi|14532708|gb|AAK64155.1| unknown protein
[Arabidopsis thaliana] gi|19310729|gb|AAL85095.1|
unknown protein [Arabidopsis thaliana]
gi|20197120|gb|AAD11993.2| expressed protein
[Arabidopsis thaliana] gi|330254848|gb|AEC09942.1|
transmembrane amino acid transporter family protein
[Arabidopsis thaliana]
Length = 536
Score = 150 bits (380), Expect = 1e-34
Identities = 74/110 (67%), Positives = 91/110 (82%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP R+S++ W ++LLRTALV S+V AF+IPFFGL+MALIGSLLSILVA
Sbjct: 426 MNPLARSIEELLPERMSENIWCFLLLRTALVASSVCSAFLIPFFGLMMALIGSLLSILVA 485
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
+IMPALCF+KI+G KAT+TQMI+S I+ GV+ LGTYSS++ I Y
Sbjct: 486 IIMPALCFIKIMGNKATRTQMILSSIIVAIGVVSGTLGTYSSVAKIIRNY 535
>gb|AAN31931.1| unknown protein [Arabidopsis thaliana]
Length = 407
Score = 150 bits (380), Expect = 1e-34
Identities = 74/110 (67%), Positives = 91/110 (82%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP R+S++ W ++LLRTALV S+V AF+IPFFGL+MALIGSLLSILVA
Sbjct: 297 MNPLARSIEELLPERMSENIWCFLLLRTALVASSVCSAFLIPFFGLMMALIGSLLSILVA 356
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
+IMPALCF+KI+G KAT+TQMI+S I+ GV+ LGTYSS++ I Y
Sbjct: 357 IIMPALCFIKIMGNKATRTQMILSSIIVAIGVVSGTLGTYSSVAKIIRNY 406
>ref|XP_006573773.1| PREDICTED: vesicular inhibitory amino acid transporter-like isoform
X3 [Glycine max]
Length = 502
Score = 150 bits (379), Expect = 2e-34
Identities = 75/110 (68%), Positives = 90/110 (81%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS ++ +ILLRTALV+STV AF+IPFFG VMALIGSL S+LV+
Sbjct: 393 MNPLARSLEELLPDRISSTYRCFILLRTALVVSTVCAAFLIPFFGFVMALIGSLFSVLVS 452
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VIMP+LCF+KI+GKKAT TQ+ +S+ I FGVIC LGTYSS+ I N Y
Sbjct: 453 VIMPSLCFMKIVGKKATATQVALSVVITTFGVICGILGTYSSVQNIVNSY 502
>ref|XP_006573771.1| PREDICTED: vesicular inhibitory amino acid transporter-like isoform
X1 [Glycine max]
Length = 534
Score = 150 bits (379), Expect = 2e-34
Identities = 75/110 (68%), Positives = 90/110 (81%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR +EELLP RIS ++ +ILLRTALV+STV AF+IPFFG VMALIGSL S+LV+
Sbjct: 425 MNPLARSLEELLPDRISSTYRCFILLRTALVVSTVCAAFLIPFFGFVMALIGSLFSVLVS 484
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VIMP+LCF+KI+GKKAT TQ+ +S+ I FGVIC LGTYSS+ I N Y
Sbjct: 485 VIMPSLCFMKIVGKKATATQVALSVVITTFGVICGILGTYSSVQNIVNSY 534
>ref|XP_002881760.1| amino acid transporter family protein [Arabidopsis lyrata subsp.
lyrata] gi|297327599|gb|EFH58019.1| amino acid
transporter family protein [Arabidopsis lyrata subsp.
lyrata]
Length = 534
Score = 150 bits (379), Expect = 2e-34
Identities = 72/110 (65%), Positives = 92/110 (83%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP R+S++ W ++LLRTALV S+V AF+IPFFGL+MALIGSLLSILVA
Sbjct: 424 MNPLARSIEELLPDRMSENIWCFLLLRTALVASSVCSAFLIPFFGLMMALIGSLLSILVA 483
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
++MPALCF+KI+G KAT+TQMI+S +I+ G++ LGTYSS++ I Y
Sbjct: 484 IVMPALCFIKIMGNKATRTQMILSSTIVAIGLVSGTLGTYSSVAKIIRNY 533
>ref|XP_006293964.1| hypothetical protein CARUB_v10022954mg [Capsella rubella]
gi|482562672|gb|EOA26862.1| hypothetical protein
CARUB_v10022954mg [Capsella rubella]
Length = 538
Score = 150 bits (378), Expect = 2e-34
Identities = 73/110 (66%), Positives = 91/110 (82%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP +S++ W ++LLRTALV S+V AF+IPFFGLVMALIGSLLSILVA
Sbjct: 428 MNPLARSIEELLPPSMSENIWCFLLLRTALVASSVCSAFLIPFFGLVMALIGSLLSILVA 487
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
++MPALCF+KI+G KAT+TQMI+S +I+ GV+ LGTYSS++ I Y
Sbjct: 488 IVMPALCFMKIMGNKATRTQMILSSTIVAIGVVSGTLGTYSSVAKIIRNY 537
>ref|XP_004305687.1| PREDICTED: vacuolar amino acid transporter 1-like [Fragaria vesca
subsp. vesca]
Length = 532
Score = 149 bits (375), Expect = 5e-34
Identities = 74/110 (67%), Positives = 92/110 (83%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEELLP +S+SFW +ILLRTALV+STV AF+IPFFGLVMALIGSLLS+LV+
Sbjct: 423 MNPLARSIEELLPVGLSNSFWCFILLRTALVISTVGAAFLIPFFGLVMALIGSLLSVLVS 482
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQY 332
VI+PALCFL+I G KA++ Q++ S+++ FGV+ A LGTYSS+S I Y
Sbjct: 483 VIVPALCFLRIAGMKASRIQVVSSITVAAFGVVAAILGTYSSVSKIVKSY 532
>gb|EPS65847.1| amino acid transporter family protein, partial [Genlisea aurea]
Length = 477
Score = 147 bits (371), Expect = 1e-33
Identities = 75/109 (68%), Positives = 88/109 (80%)
Frame = +3
Query: 3 MNPLARGIEELLPSRISDSFWFYILLRTALVLSTVFFAFIIPFFGLVMALIGSLLSILVA 182
MNPLAR IEEL+P +S S WFY+ LR LV STV AF+IPFF LVMALIGSLLSILVA
Sbjct: 369 MNPLARSIEELVPWGVSSSLWFYVALRFLLVASTVCIAFVIPFFALVMALIGSLLSILVA 428
Query: 183 VIMPALCFLKILGKKATKTQMIMSMSIIVFGVICAALGTYSSLSGIANQ 329
+IMPALCFLKI GKKA+++Q+IMS I+V G++ A +GTYSSL IA Q
Sbjct: 429 IIMPALCFLKIAGKKASRSQVIMSNGIVVVGILSAGVGTYSSLFKIARQ 477