BLASTX nr result
ID: Mentha25_contig00026789
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha25_contig00026789
(347 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAM64855.1| peptidase M48 [Beta vulgaris] 74 2e-11
dbj|BAM64817.1| metalloendopeptidase-like protein [Beta vulgaris] 68 1e-09
dbj|BAM64832.1| peptidase M48 [Beta vulgaris] 65 1e-08
dbj|BAM64816.1| metalloendopeptidase-like protein [Beta vulgaris... 65 1e-08
ref|XP_002947008.1| hypothetical protein VOLCADRAFT_116256 [Volv... 60 2e-07
gb|EGX51875.1| hypothetical protein AOL_s00043g609 [Arthrobotrys... 59 5e-07
gb|EMS64722.1| Mitochondrial metalloendopeptidase OMA1 [Triticum... 59 7e-07
ref|YP_004441279.1| peptidase M48 Ste24p [Porphyromonas asacchar... 58 2e-06
ref|WP_004330428.1| hypothetical protein [Porphyromonas asacchar... 58 2e-06
ref|WP_022129706.1| peptidase M48 Ste24p [Bacteroides sp. CAG:53... 57 2e-06
ref|WP_009124836.1| hypothetical protein [Bacteroides fluxus] gi... 57 2e-06
gb|EPE27585.1| hypothetical protein GLAREA_04376 [Glarea lozoyen... 57 3e-06
ref|WP_006927654.1| peptidase M48 [Caldithrix abyssi] gi|3717188... 57 3e-06
ref|XP_003573129.1| PREDICTED: mitochondrial metalloendopeptidas... 57 3e-06
gb|EMT05584.1| Mitochondrial metalloendopeptidase OMA1 [Aegilops... 56 4e-06
gb|EGO61038.1| hypothetical protein NEUTE1DRAFT_58041 [Neurospor... 56 4e-06
ref|XP_961653.2| hypothetical protein NCU01041 [Neurospora crass... 56 4e-06
emb|CAB88499.1| conserved hypothetical protein [Neurospora crass... 56 4e-06
ref|WP_022854284.1| hypothetical protein [Thermodesulfatator atl... 56 6e-06
ref|YP_004626071.1| peptidase M48 Ste24p [Thermodesulfatator ind... 56 6e-06
>dbj|BAM64855.1| peptidase M48 [Beta vulgaris]
Length = 434
Score = 73.9 bits (180), Expect = 2e-11
Identities = 51/128 (39%), Positives = 71/128 (55%), Gaps = 14/128 (10%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPG---LAIFMQFLDWAIRL-HLKPAISKFLTR 171
SDAE+A IAH+V H++ARH EH + L L I+M + AISK L+R
Sbjct: 254 SDAELATIIAHQVGHAVARHEAEHWTTLLWSILLVIYMTIFQYLFTAPEFANAISKLLSR 313
Query: 172 FAYSQRRFAKKVFKHYWLIPITTS----------VFLIWFPDSRRREKEADYIGLILMAV 321
Q+ + + + L+P TT VF+++F R+E EAD+IG++LMA
Sbjct: 314 HPLLQKVWKIIQARFHQLLPRTTLHLGFLGLSSLVFILYFG---RKEIEADHIGVLLMAS 370
Query: 322 AGYDPRVA 345
AGYDPRVA
Sbjct: 371 AGYDPRVA 378
>dbj|BAM64817.1| metalloendopeptidase-like protein [Beta vulgaris]
Length = 428
Score = 68.2 bits (165), Expect = 1e-09
Identities = 49/128 (38%), Positives = 70/128 (54%), Gaps = 14/128 (10%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKP----AISKFLTR 171
SDAE+A IAH+V H++ARH EH + L ++ ++ L P A SK L R
Sbjct: 248 SDAELATIIAHQVGHAVARHEAEHWTALFWWSMLGFYVTLFEILFTAPEFANARSKLLLR 307
Query: 172 FAYSQRRFAKKVFKHYWLIPITTS----------VFLIWFPDSRRREKEADYIGLILMAV 321
Q+ + + + L+P TT VF+++F R+E EAD+IG++LMA
Sbjct: 308 HPLLQKVWKIIQARFHQLLPRTTLRLGFVGLSSLVFILYFG---RKEIEADHIGVLLMAS 364
Query: 322 AGYDPRVA 345
AGYDPRVA
Sbjct: 365 AGYDPRVA 372
>dbj|BAM64832.1| peptidase M48 [Beta vulgaris]
Length = 429
Score = 65.1 bits (157), Expect = 1e-08
Identities = 49/127 (38%), Positives = 71/127 (55%), Gaps = 13/127 (10%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKP----AISKFLTR 171
SDAE+A IAH+V H++ARH E + L I + + + I L +P A SK L R
Sbjct: 251 SDAELATIIAHQVGHAVARHEAEDSTAFFWLLISLNVILFKI-LFTEPESANARSKLLLR 309
Query: 172 FAYSQRRFAKKVFKHYWLIPIT---------TSVFLIWFPDSRRREKEADYIGLILMAVA 324
Q+ + + L+P T +SVF++++ R+E EAD+IG++LMA A
Sbjct: 310 HPLLQKVWKIIQARAPQLLPRTICLSLVGLFSSVFILYYG---RKEIEADHIGVLLMASA 366
Query: 325 GYDPRVA 345
GYDPRVA
Sbjct: 367 GYDPRVA 373
>dbj|BAM64816.1| metalloendopeptidase-like protein [Beta vulgaris]
gi|410129755|dbj|BAM64833.1| peptidase M48 [Beta
vulgaris]
Length = 431
Score = 65.1 bits (157), Expect = 1e-08
Identities = 49/127 (38%), Positives = 71/127 (55%), Gaps = 13/127 (10%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKP----AISKFLTR 171
SDAE+A IAH+V H++ARH E + L I + + + I L +P A SK L R
Sbjct: 253 SDAELATIIAHQVGHAVARHEAEDSTAFFWLLISLNVILFKI-LFTEPESANARSKLLLR 311
Query: 172 FAYSQRRFAKKVFKHYWLIPIT---------TSVFLIWFPDSRRREKEADYIGLILMAVA 324
Q+ + + L+P T +SVF++++ R+E EAD+IG++LMA A
Sbjct: 312 HPLLQKVWKIIQARAPQLLPRTICLSLVGLFSSVFILYYG---RKEIEADHIGVLLMASA 368
Query: 325 GYDPRVA 345
GYDPRVA
Sbjct: 369 GYDPRVA 375
>ref|XP_002947008.1| hypothetical protein VOLCADRAFT_116256 [Volvox carteri f.
nagariensis] gi|300268052|gb|EFJ52234.1| hypothetical
protein VOLCADRAFT_116256 [Volvox carteri f.
nagariensis]
Length = 422
Score = 60.5 bits (145), Expect = 2e-07
Identities = 44/114 (38%), Positives = 55/114 (48%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
SDAE+AA +AHEV H +ARH E+LSRL +F + A+RL L A+
Sbjct: 257 SDAELAAVLAHEVGHVLARHPAENLSRLNVWLLF----NTALRLTLGFAL---------- 302
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
P + P+SRR E EAD IGL LMA A +DP A
Sbjct: 303 ---------------PDVAMYLALMLPNSRRNEHEADVIGLHLMAQACFDPSAA 341
>gb|EGX51875.1| hypothetical protein AOL_s00043g609 [Arthrobotrys oligospora ATCC
24927]
Length = 362
Score = 59.3 bits (142), Expect = 5e-07
Identities = 38/117 (32%), Positives = 55/117 (47%), Gaps = 4/117 (3%)
Frame = +1
Query: 7 DAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYSQ 186
D +AA + HE+ H++ARH E +SR IF+ W + L
Sbjct: 206 DDGLAAVLGHEIGHNVARHLGEQISR----GIFLIAAAWVVEL----------------- 244
Query: 187 RRFAKKVFKHYWLIPITTSVFLIWF----PDSRRREKEADYIGLILMAVAGYDPRVA 345
+W +P S+ L+ F P SR +E EAD+IGL+LMA + YDP+ A
Sbjct: 245 ----------FWGVPGDMSLHLLQFAIDMPKSRAQESEADHIGLLLMAKSCYDPKAA 291
>gb|EMS64722.1| Mitochondrial metalloendopeptidase OMA1 [Triticum urartu]
Length = 409
Score = 58.9 bits (141), Expect = 7e-07
Identities = 38/114 (33%), Positives = 58/114 (50%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+DAEIA + HEV+H+IARH E L++ + + FL I
Sbjct: 265 TDAEIATVLGHEVAHAIARHTAEGLTKNMWILMLTVFLGIFI-----------------D 307
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ + K+ K +L+ P SR+ E EAD+IG++L+A AG+DPR+A
Sbjct: 308 EPKMIDKLAK-----------YLLSLPFSRKMEIEADHIGILLLAAAGFDPRIA 350
>ref|YP_004441279.1| peptidase M48 Ste24p [Porphyromonas asaccharolytica DSM 20707]
gi|503525605|ref|WP_013759809.1| hypothetical protein
[Porphyromonas asaccharolytica]
gi|332176421|gb|AEE12111.1| peptidase M48 Ste24p
[Porphyromonas asaccharolytica DSM 20707]
Length = 320
Score = 57.8 bits (138), Expect = 2e-06
Identities = 40/114 (35%), Positives = 56/114 (49%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+D E+AA IAHEVSH++A+H E +SR L + LT S
Sbjct: 128 TDDELAAVIAHEVSHAVAKHANERISREM----------------LTQLGGQVLTGMVGS 171
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
Q + + + I S L+ P R++E EAD IG++ MA+AGYDP A
Sbjct: 172 QSAALGGILQQVYPIG---SQLLVSLPYGRKQEYEADKIGMVFMAMAGYDPAAA 222
>ref|WP_004330428.1| hypothetical protein [Porphyromonas asaccharolytica]
gi|312924471|gb|EFR35242.1| peptidase, M48 family
[Porphyromonas asaccharolytica PR426713P-I]
Length = 320
Score = 57.8 bits (138), Expect = 2e-06
Identities = 40/114 (35%), Positives = 56/114 (49%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+D E+AA IAHEVSH++A+H E +SR L + LT S
Sbjct: 128 TDDELAAVIAHEVSHAVAKHANERISREM----------------LTQLGGQVLTGMVGS 171
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
Q + + + I S L+ P R++E EAD IG++ MA+AGYDP A
Sbjct: 172 QSAALGGILQQVYPIG---SQLLVSLPYGRKQEYEADKIGMVFMAMAGYDPAAA 222
>ref|WP_022129706.1| peptidase M48 Ste24p [Bacteroides sp. CAG:530]
gi|524317090|emb|CDA77903.1| peptidase M48 Ste24p
[Bacteroides sp. CAG:530]
Length = 264
Score = 57.4 bits (137), Expect = 2e-06
Identities = 39/114 (34%), Positives = 59/114 (51%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
SD E+A I HEV+H++ARH E +S+ Q L S+ L++ + +
Sbjct: 125 SDDELAVVIGHEVAHAVARHSNERISQEMLAQYGAQIL------------SQSLSQKSAA 172
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ A KV+ + + + P SR+ E EADY+GLI M +AGY+P VA
Sbjct: 173 IQNMANKVYG-------IGAQYGVVLPFSRKHESEADYMGLIFMTMAGYNPDVA 219
>ref|WP_009124836.1| hypothetical protein [Bacteroides fluxus]
gi|328530612|gb|EGF57470.1| peptidase, M48 family
[Bacteroides fluxus YIT 12057]
Length = 302
Score = 57.4 bits (137), Expect = 2e-06
Identities = 37/114 (32%), Positives = 62/114 (54%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
SD E+A I HEV+H++A+H E +S+ I Q+ +++ L++ + +
Sbjct: 164 SDDELAVVIGHEVAHAVAKHSNERMSQ----QILAQYG--------AQILNQSLSQKSTA 211
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ AK+V+ + + + P SR+ E EADY+GLI M +AGY+P VA
Sbjct: 212 MQTIAKQVYG-------VGAQYGVMLPFSRKHESEADYMGLIFMRMAGYNPDVA 258
>gb|EPE27585.1| hypothetical protein GLAREA_04376 [Glarea lozoyensis ATCC 20868]
Length = 380
Score = 57.0 bits (136), Expect = 3e-06
Identities = 40/115 (34%), Positives = 58/115 (50%)
Frame = +1
Query: 1 ESDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAY 180
++D +AA + HE++H++ARH E +S + I +Q + W I T +
Sbjct: 210 KNDDGLAAILGHEIAHNLARHSNEAMSSM----ILLQPVRWMF-------IFLDSTGYTG 258
Query: 181 SQRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
R +F + L+ P SRR+E EADYIGLI+MA A YDPR A
Sbjct: 259 GLGRLIGDLFLDFGLMK----------PASRRQESEADYIGLIMMAKACYDPRAA 303
>ref|WP_006927654.1| peptidase M48 [Caldithrix abyssi] gi|371718858|gb|EHO40629.1|
peptidase M48 Ste24p [Caldithrix abyssi DSM 13497]
Length = 265
Score = 56.6 bits (135), Expect = 3e-06
Identities = 40/117 (34%), Positives = 60/117 (51%), Gaps = 2/117 (1%)
Frame = +1
Query: 1 ESDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISK--FLTRF 174
+++ +A + HE++H++A H E +S+ GL + + L + L KP ++ FLT F
Sbjct: 124 QNETGLAVVMGHEIAHAVAEHGNERMSQ--GLLVQLGGLALSKALEEKPEETRNLFLTAF 181
Query: 175 AYSQRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ V L P SR EKEAD++GLI MA+AGYDPR A
Sbjct: 182 GIGSQ----------------VGVLL---PYSRLHEKEADHLGLIFMAMAGYDPREA 219
>ref|XP_003573129.1| PREDICTED: mitochondrial metalloendopeptidase OMA1-like
[Brachypodium distachyon]
Length = 314
Score = 56.6 bits (135), Expect = 3e-06
Identities = 39/115 (33%), Positives = 55/115 (47%)
Frame = +1
Query: 1 ESDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAY 180
++DAE+A + HEV H IARH E +++ W L L FL F
Sbjct: 161 KTDAELATVLGHEVGHIIARHAAEQITKNM----------WIFILEL------FLLIFCD 204
Query: 181 SQRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
K + T + ++ P SR+ E EAD+IG++L+A AGYDPR A
Sbjct: 205 DDENNPKNI--------ATLTELILKKPFSRKMELEADHIGVLLLAAAGYDPRDA 251
>gb|EMT05584.1| Mitochondrial metalloendopeptidase OMA1 [Aegilops tauschii]
Length = 397
Score = 56.2 bits (134), Expect = 4e-06
Identities = 48/136 (35%), Positives = 60/136 (44%), Gaps = 22/136 (16%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+DAEIA IAHEV H IARH E + IF +L RL + P + + F
Sbjct: 208 TDAEIATIIAHEVGHIIARHSSEMIK------IFKWWLP--TRLLVTPLLQRLSQVFGNP 259
Query: 184 QRRFAKKVFKHYWLIPITTSVFLI-----------WFP-----------DSRRREKEADY 297
Q F F +P VFL+ W P S+R E EADY
Sbjct: 260 QL-FGPLPFG----VPTKIPVFLVFVWPAPTDHLCWIPICPSAMSYCQLGSQRNELEADY 314
Query: 298 IGLILMAVAGYDPRVA 345
IG++L A AG+DP A
Sbjct: 315 IGMLLQAAAGFDPHAA 330
>gb|EGO61038.1| hypothetical protein NEUTE1DRAFT_58041 [Neurospora tetrasperma FGSC
2508] gi|350293870|gb|EGZ74955.1| hypothetical protein
NEUTE2DRAFT_82805 [Neurospora tetrasperma FGSC 2509]
Length = 435
Score = 56.2 bits (134), Expect = 4e-06
Identities = 37/114 (32%), Positives = 59/114 (51%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+D+++A + HE++H++A H E +S+ GL I + L + L + + +FA +
Sbjct: 270 TDSQLATVLGHEIAHNVADHVGERMSQTLGLNILLYSL---VVLCGALGLGAIIAQFAGA 326
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
W + S P SR++E EADYIGL++MA A YDPR A
Sbjct: 327 ------------WALDTAISN-----PMSRKQESEADYIGLMMMAEACYDPREA 363
>ref|XP_961653.2| hypothetical protein NCU01041 [Neurospora crassa OR74A]
Length = 410
Score = 56.2 bits (134), Expect = 4e-06
Identities = 37/114 (32%), Positives = 59/114 (51%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+D+++A + HE++H++A H E +S+ GL I + L + L + + +FA +
Sbjct: 275 TDSQLATVLGHEIAHNVADHVGERMSQTLGLNILLYSL---VVLCGALGLGAIIAQFAGA 331
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
W + S P SR++E EADYIGL++MA A YDPR A
Sbjct: 332 ------------WALDTAISN-----PMSRKQESEADYIGLMMMAEACYDPREA 368
>emb|CAB88499.1| conserved hypothetical protein [Neurospora crassa]
gi|553135734|gb|EAA32417.3| mitochondrial
metalloendopeptidase OMA1 [Neurospora crassa OR74A]
Length = 440
Score = 56.2 bits (134), Expect = 4e-06
Identities = 37/114 (32%), Positives = 59/114 (51%)
Frame = +1
Query: 4 SDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAYS 183
+D+++A + HE++H++A H E +S+ GL I + L + L + + +FA +
Sbjct: 275 TDSQLATVLGHEIAHNVADHVGERMSQTLGLNILLYSL---VVLCGALGLGAIIAQFAGA 331
Query: 184 QRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
W + S P SR++E EADYIGL++MA A YDPR A
Sbjct: 332 ------------WALDTAISN-----PMSRKQESEADYIGLMMMAEACYDPREA 368
>ref|WP_022854284.1| hypothetical protein [Thermodesulfatator atlanticus]
Length = 279
Score = 55.8 bits (133), Expect = 6e-06
Identities = 44/115 (38%), Positives = 61/115 (53%)
Frame = +1
Query: 1 ESDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAY 180
+++A +A ++HE++H+IARH E +S + +A F + L A L LK +K + AY
Sbjct: 120 KNEAGLATVLSHEIAHAIARHGAERMS-IAMVATFGEAL-LASMLDLKNPETKRIFLTAY 177
Query: 181 SQRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ T V L P SR +E EAD IGL LMA AGYDPR A
Sbjct: 178 G--------------LGATVGVLL---PYSREQEYEADTIGLYLMAKAGYDPREA 215
>ref|YP_004626071.1| peptidase M48 Ste24p [Thermodesulfatator indicus DSM 15286]
gi|503673773|ref|WP_013907849.1| hypothetical protein
[Thermodesulfatator indicus] gi|335359426|gb|AEH45107.1|
peptidase M48 Ste24p [Thermodesulfatator indicus DSM
15286]
Length = 279
Score = 55.8 bits (133), Expect = 6e-06
Identities = 44/115 (38%), Positives = 63/115 (54%)
Frame = +1
Query: 1 ESDAEIAAAIAHEVSHSIARHYPEHLSRLPGLAIFMQFLDWAIRLHLKPAISKFLTRFAY 180
+++A +A +AHE++H+IARH E +S + +A F + L A L L + ++ L AY
Sbjct: 120 QNEAGLATVLAHEIAHAIARHGAERMS-IAMVAAFGEVLA-AELLDLNNSRTRELFMAAY 177
Query: 181 SQRRFAKKVFKHYWLIPITTSVFLIWFPDSRRREKEADYIGLILMAVAGYDPRVA 345
+ +V LI P SR++E EAD IGL LMA AGYDPR A
Sbjct: 178 G----------------LGATVGLI-LPYSRKQEYEADTIGLYLMAKAGYDPREA 215