BLASTX nr result
ID: Mentha24_contig00019520
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha24_contig00019520
(316 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU44942.1| hypothetical protein MIMGU_mgv1a002188mg [Mimulus... 159 5e-37
gb|EYU26387.1| hypothetical protein MIMGU_mgv1a002272mg [Mimulus... 154 2e-35
ref|XP_004248265.1| PREDICTED: uncharacterized protein LOC101267... 151 8e-35
ref|XP_006592909.1| PREDICTED: probable ubiquitin-conjugating en... 149 3e-34
ref|XP_006592908.1| PREDICTED: probable ubiquitin-conjugating en... 149 3e-34
ref|XP_006362957.1| PREDICTED: probable ubiquitin-conjugating en... 149 3e-34
ref|XP_006594771.1| PREDICTED: probable ubiquitin-conjugating en... 149 4e-34
ref|XP_006594770.1| PREDICTED: probable ubiquitin-conjugating en... 149 4e-34
ref|XP_007211379.1| hypothetical protein PRUPE_ppa002674mg [Prun... 146 3e-33
ref|XP_004160617.1| PREDICTED: probable ubiquitin-conjugating en... 146 3e-33
ref|XP_004141363.1| PREDICTED: probable ubiquitin-conjugating en... 146 3e-33
ref|XP_004511247.1| PREDICTED: probable ubiquitin-conjugating en... 145 4e-33
ref|XP_007149464.1| hypothetical protein PHAVU_005G072300g [Phas... 145 6e-33
ref|XP_004487698.1| PREDICTED: probable ubiquitin-conjugating en... 145 6e-33
ref|XP_002317497.2| hypothetical protein POPTR_0011s11950g [Popu... 144 1e-32
ref|XP_002531713.1| ubiquitin conjugating enzyme, putative [Rici... 144 1e-32
gb|EXB51379.1| putative ubiquitin-conjugating enzyme E2 26 [Moru... 144 2e-32
ref|XP_002882934.1| ubiquitin-conjugating enzyme 23 [Arabidopsis... 144 2e-32
ref|XP_006370262.1| hypothetical protein POPTR_0001s41090g [Popu... 143 3e-32
ref|XP_007021479.1| Ubiquitin-conjugating enzyme 25, putative is... 142 5e-32
>gb|EYU44942.1| hypothetical protein MIMGU_mgv1a002188mg [Mimulus guttatus]
Length = 703
Score = 159 bits (401), Expect = 5e-37
Identities = 72/105 (68%), Positives = 85/105 (80%)
Frame = -2
Query: 315 KKVVDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAE 136
++ +DEI+ KFE F KF+TVEDYSDHYY + A K W+K +QDEWK+LEKNLP
Sbjct: 395 REKLDEILQKFERFEKFDTVEDYSDHYYGRYGYSGKQAPKGWAKSVQDEWKILEKNLPDM 454
Query: 135 IYVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
I+VR YESRMDL+RAVIIGAEGTPYHDGLFFFDI FP+NYP +PP
Sbjct: 455 IFVRVYESRMDLLRAVIIGAEGTPYHDGLFFFDIFFPANYPDVPP 499
>gb|EYU26387.1| hypothetical protein MIMGU_mgv1a002272mg [Mimulus guttatus]
Length = 692
Score = 154 bits (388), Expect = 2e-35
Identities = 69/102 (67%), Positives = 83/102 (81%)
Frame = -2
Query: 306 VDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYV 127
VD+ V FE F KF+TVED+SDH+Y + A K W+KRIQ+EWK+LEK+LPAEI+V
Sbjct: 387 VDKSVQMFEAFKKFDTVEDFSDHHYCNSGSSAKQAPKNWAKRIQEEWKILEKDLPAEIFV 446
Query: 126 RAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
R YESRMDL+RAVIIGAEGTPY DGLFFFD+ FPS+YP +PP
Sbjct: 447 RVYESRMDLLRAVIIGAEGTPYQDGLFFFDVFFPSSYPNVPP 488
>ref|XP_004248265.1| PREDICTED: uncharacterized protein LOC101267744 [Solanum
lycopersicum]
Length = 667
Score = 151 bits (382), Expect = 8e-35
Identities = 65/102 (63%), Positives = 84/102 (82%)
Frame = -2
Query: 306 VDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYV 127
VDEI+ F+ F KF+TVED+SDH+Y +Q + SK W+K+IQ+EWK+LE +LP I+V
Sbjct: 375 VDEILKNFQAFKKFDTVEDHSDHFYSRQASSGNLPSKNWAKKIQEEWKILENDLPDTIFV 434
Query: 126 RAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
R YESRMDL+RAVI+GA+GTPYHDGL+FFD+ FPSNYP +PP
Sbjct: 435 RVYESRMDLLRAVIMGADGTPYHDGLYFFDVFFPSNYPNVPP 476
>ref|XP_006592909.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like isoform
X2 [Glycine max]
Length = 702
Score = 149 bits (377), Expect = 3e-34
Identities = 63/101 (62%), Positives = 84/101 (83%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
+EI+ KF +F +F+TVED SDH++F+ ++ K W+K+IQ+EW++LEK+LPA I+VR
Sbjct: 402 EEILRKFRSFKQFDTVEDISDHHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVR 461
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
+ESRMDL+RAVIIGAEGTPYHDGLFFFD+ FPS YP +PP
Sbjct: 462 VFESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSAYPNVPP 502
>ref|XP_006592908.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like isoform
X1 [Glycine max]
Length = 704
Score = 149 bits (377), Expect = 3e-34
Identities = 63/101 (62%), Positives = 84/101 (83%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
+EI+ KF +F +F+TVED SDH++F+ ++ K W+K+IQ+EW++LEK+LPA I+VR
Sbjct: 404 EEILRKFRSFKQFDTVEDISDHHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVR 463
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
+ESRMDL+RAVIIGAEGTPYHDGLFFFD+ FPS YP +PP
Sbjct: 464 VFESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSAYPNVPP 504
>ref|XP_006362957.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like
[Solanum tuberosum]
Length = 695
Score = 149 bits (377), Expect = 3e-34
Identities = 64/100 (64%), Positives = 83/100 (83%)
Frame = -2
Query: 300 EIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVRA 121
EI+ KF+ F KF+TVED+SDH+Y +Q + SK W+K+IQ+EWK+LE +LP I+VR
Sbjct: 390 EILKKFQVFKKFDTVEDHSDHFYSRQASSGNLPSKNWAKKIQEEWKILENDLPDTIFVRV 449
Query: 120 YESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDL+RAVI+GA+GTPYHDGL+FFD+ FPSNYP +PP
Sbjct: 450 YESRMDLLRAVIMGADGTPYHDGLYFFDVFFPSNYPSVPP 489
>ref|XP_006594771.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like isoform
X2 [Glycine max]
Length = 721
Score = 149 bits (376), Expect = 4e-34
Identities = 63/101 (62%), Positives = 84/101 (83%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
+EI+ K ++F +F+TVED SDH++F+ ++ K W+K+IQ+EW++LEK+LPA I+VR
Sbjct: 421 EEILRKLQSFKQFDTVEDTSDHHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVR 480
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDL+RAVIIGAEGTPYHDGLFFFD+ FPS YP +PP
Sbjct: 481 VYESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSAYPNVPP 521
>ref|XP_006594770.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like isoform
X1 [Glycine max]
Length = 723
Score = 149 bits (376), Expect = 4e-34
Identities = 63/101 (62%), Positives = 84/101 (83%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
+EI+ K ++F +F+TVED SDH++F+ ++ K W+K+IQ+EW++LEK+LPA I+VR
Sbjct: 423 EEILRKLQSFKQFDTVEDTSDHHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVR 482
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDL+RAVIIGAEGTPYHDGLFFFD+ FPS YP +PP
Sbjct: 483 VYESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSAYPNVPP 523
>ref|XP_007211379.1| hypothetical protein PRUPE_ppa002674mg [Prunus persica]
gi|462407244|gb|EMJ12578.1| hypothetical protein
PRUPE_ppa002674mg [Prunus persica]
Length = 646
Score = 146 bits (369), Expect = 3e-33
Identities = 62/102 (60%), Positives = 81/102 (79%)
Frame = -2
Query: 306 VDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYV 127
+DE + KF+ F +F+T+ED+SDH+Y + + K W+KRIQ+EWK+LEK+LP I+V
Sbjct: 339 IDEFIEKFKGFKQFDTIEDHSDHHYTSRGSSTKQPPKNWAKRIQEEWKILEKDLPDTIFV 398
Query: 126 RAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
R YE+RMDL+RAVI+GAEGTPYHDGLFFFD+ FPS YP PP
Sbjct: 399 RVYETRMDLLRAVIVGAEGTPYHDGLFFFDVFFPSGYPNAPP 440
>ref|XP_004160617.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like
[Cucumis sativus]
Length = 615
Score = 146 bits (369), Expect = 3e-33
Identities = 62/101 (61%), Positives = 81/101 (80%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
D+ + FE F +F+T++D+SDH+Y + + ASKKW+K+IQD+WK+L+ +LP I+VR
Sbjct: 311 DKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVR 370
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDLMRAVIIGA+GTPYHDGLFFFDI FP YP +PP
Sbjct: 371 VYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPP 411
>ref|XP_004141363.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like
[Cucumis sativus]
Length = 646
Score = 146 bits (369), Expect = 3e-33
Identities = 62/101 (61%), Positives = 81/101 (80%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
D+ + FE F +F+T++D+SDH+Y + + ASKKW+K+IQD+WK+L+ +LP I+VR
Sbjct: 342 DKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVR 401
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDLMRAVIIGA+GTPYHDGLFFFDI FP YP +PP
Sbjct: 402 VYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPP 442
>ref|XP_004511247.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like [Cicer
arietinum]
Length = 749
Score = 145 bits (367), Expect = 4e-33
Identities = 64/104 (61%), Positives = 83/104 (79%)
Frame = -2
Query: 312 KVVDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEI 133
+V DEI+ KF+ F +F+T++D SDHY+ + + SK W KRIQ+EWK+LEK+LP I
Sbjct: 452 EVRDEILKKFQNFKQFDTIDDTSDHYFTRANVSMTQHSKNWVKRIQEEWKLLEKDLPDSI 511
Query: 132 YVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
+VR YESR+DL+RAVIIGAEGTPYHDGLFFFD+ FP +YP +PP
Sbjct: 512 FVRVYESRIDLLRAVIIGAEGTPYHDGLFFFDVFFPRDYPNVPP 555
>ref|XP_007149464.1| hypothetical protein PHAVU_005G072300g [Phaseolus vulgaris]
gi|561022728|gb|ESW21458.1| hypothetical protein
PHAVU_005G072300g [Phaseolus vulgaris]
Length = 702
Score = 145 bits (366), Expect = 6e-33
Identities = 62/101 (61%), Positives = 81/101 (80%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
DEI++KF++F +F+TVED SDHY+ + + K W+K+IQ+EWK+L+K+LP I+VR
Sbjct: 397 DEIMNKFQSFKQFDTVEDTSDHYFVRNSSSMKQPPKDWAKKIQEEWKILQKDLPDSIFVR 456
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YES MDL+RAVIIGAEGTPYHDGLFFFD+ FP YP +PP
Sbjct: 457 VYESNMDLLRAVIIGAEGTPYHDGLFFFDVLFPCGYPSVPP 497
>ref|XP_004487698.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 25-like [Cicer
arietinum]
Length = 670
Score = 145 bits (366), Expect = 6e-33
Identities = 64/104 (61%), Positives = 81/104 (77%)
Frame = -2
Query: 312 KVVDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEI 133
+ V+E + KF +F F+TV D SDH++ ++ K W+K+IQ+EWK+LEK+LP I
Sbjct: 377 ETVNETLRKFRSFKHFDTVIDTSDHFFMNYNSSTKQNPKSWAKKIQEEWKILEKDLPDTI 436
Query: 132 YVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
+VR YESRMDL+RAVIIGAEGTPYHDGLFFFDIHFPS YP +PP
Sbjct: 437 FVRVYESRMDLLRAVIIGAEGTPYHDGLFFFDIHFPSGYPNVPP 480
>ref|XP_002317497.2| hypothetical protein POPTR_0011s11950g [Populus trichocarpa]
gi|550328192|gb|EEE98109.2| hypothetical protein
POPTR_0011s11950g [Populus trichocarpa]
Length = 613
Score = 144 bits (364), Expect = 1e-32
Identities = 61/101 (60%), Positives = 82/101 (81%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
D+I+ KF+ F +F+TVED+SDH+Y + + K W+KRIQ+EW++LE +LP I+VR
Sbjct: 320 DDILRKFQLFKRFDTVEDHSDHHYTSKGSLMKQPPKTWAKRIQEEWRILENDLPDSIFVR 379
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YE+RMDL+RAVIIGAEGTPYHDGLFFFD+ FP+ YP++PP
Sbjct: 380 VYETRMDLLRAVIIGAEGTPYHDGLFFFDVFFPAGYPKVPP 420
>ref|XP_002531713.1| ubiquitin conjugating enzyme, putative [Ricinus communis]
gi|223528656|gb|EEF30672.1| ubiquitin conjugating
enzyme, putative [Ricinus communis]
Length = 647
Score = 144 bits (363), Expect = 1e-32
Identities = 62/101 (61%), Positives = 81/101 (80%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
D+I+ K++ F +F+TVED+SDH+Y + + K W+K+IQ+EW++LE +LP I+VR
Sbjct: 353 DDILSKYQNFKRFDTVEDHSDHHYASKGSSVKQPPKNWAKKIQEEWRILENDLPDGIFVR 412
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDL+RAVIIGAEGTPYHDGLFFFD+ FPS YP IPP
Sbjct: 413 IYESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPSGYPNIPP 453
>gb|EXB51379.1| putative ubiquitin-conjugating enzyme E2 26 [Morus notabilis]
Length = 730
Score = 144 bits (362), Expect = 2e-32
Identities = 64/107 (59%), Positives = 84/107 (78%), Gaps = 7/107 (6%)
Frame = -2
Query: 300 EIVHKFETFPKFETVEDYSDHYYFKQHNQFGG-------ASKKWSKRIQDEWKVLEKNLP 142
EI+ KF+ F +F+TV+D+SDH+Y + + +SK W+KRIQ+EWK+LEK+LP
Sbjct: 423 EIMRKFQLFKQFDTVDDHSDHHYTRNGSSSTSNGSSLKHSSKNWAKRIQEEWKILEKDLP 482
Query: 141 AEIYVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
I+VR YESRMDL+RAVI+GAEGTPYHDGLFFFD+ FPS+YP +PP
Sbjct: 483 DTIFVRVYESRMDLLRAVIVGAEGTPYHDGLFFFDVFFPSSYPNVPP 529
>ref|XP_002882934.1| ubiquitin-conjugating enzyme 23 [Arabidopsis lyrata subsp. lyrata]
gi|297328774|gb|EFH59193.1| ubiquitin-conjugating enzyme
23 [Arabidopsis lyrata subsp. lyrata]
Length = 605
Score = 144 bits (362), Expect = 2e-32
Identities = 64/105 (60%), Positives = 83/105 (79%)
Frame = -2
Query: 315 KKVVDEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAE 136
K+ +++ + + F +F+ VED+SDH+Y + N SK+W+KRIQDEWK+LEK+LP
Sbjct: 288 KRNMEDFLGLYLFFKRFDIVEDFSDHHYASKGNSSKQHSKEWAKRIQDEWKILEKDLPDM 347
Query: 135 IYVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
I+VRAYESRMDL+RAVIIGA+GTPYHDGLFFFDI FP YP +PP
Sbjct: 348 IFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSVPP 392
>ref|XP_006370262.1| hypothetical protein POPTR_0001s41090g [Populus trichocarpa]
gi|550349441|gb|ERP66831.1| hypothetical protein
POPTR_0001s41090g [Populus trichocarpa]
Length = 665
Score = 143 bits (360), Expect = 3e-32
Identities = 63/107 (58%), Positives = 83/107 (77%), Gaps = 6/107 (5%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQ------FGGASKKWSKRIQDEWKVLEKNLP 142
D+I+ KF+ F +F+TVED+SDH+Y + F + K W+KRIQDEW++LE +LP
Sbjct: 358 DDILRKFQQFKQFDTVEDHSDHHYTNSSSMKQVTSSFWQSPKTWAKRIQDEWRILENDLP 417
Query: 141 AEIYVRAYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
I+VR YE+RMDL+RAVIIGAEGTPYHDGLFFFD+ FP+ YP++PP
Sbjct: 418 DSIFVRVYETRMDLLRAVIIGAEGTPYHDGLFFFDVFFPAGYPKVPP 464
>ref|XP_007021479.1| Ubiquitin-conjugating enzyme 25, putative isoform 2 [Theobroma
cacao] gi|508721107|gb|EOY13004.1| Ubiquitin-conjugating
enzyme 25, putative isoform 2 [Theobroma cacao]
Length = 576
Score = 142 bits (358), Expect = 5e-32
Identities = 61/101 (60%), Positives = 81/101 (80%)
Frame = -2
Query: 303 DEIVHKFETFPKFETVEDYSDHYYFKQHNQFGGASKKWSKRIQDEWKVLEKNLPAEIYVR 124
+EI+ K + F +F+TV+D+SDH+Y K W+K+IQ+EWK+LEK+LP I+VR
Sbjct: 343 EEILSKLQLFKQFDTVDDFSDHHYASSGASTKQPPKNWAKKIQEEWKILEKDLPDTIFVR 402
Query: 123 AYESRMDLMRAVIIGAEGTPYHDGLFFFDIHFPSNYPQIPP 1
YESRMDL+RAVIIGAEGTPYHDGLFFFD+ FP++YP++PP
Sbjct: 403 VYESRMDLLRAVIIGAEGTPYHDGLFFFDVFFPASYPKVPP 443