BLASTX nr result
ID: Mentha23_contig00047424
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha23_contig00047424
(347 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004229601.1| PREDICTED: disease resistance protein RPM1-l... 92 6e-17
ref|XP_007210392.1| hypothetical protein PRUPE_ppa000961mg [Prun... 90 4e-16
ref|XP_006368520.1| hypothetical protein POPTR_0001s03710g [Popu... 89 5e-16
ref|XP_006368330.1| hypothetical protein POPTR_0001s01670g [Popu... 89 5e-16
ref|XP_006366513.1| PREDICTED: disease resistance protein RPM1-l... 89 8e-16
ref|XP_003631469.1| PREDICTED: disease resistance protein RPM1-l... 86 4e-15
emb|CAN74463.1| hypothetical protein VITISV_032893 [Vitis vinifera] 86 4e-15
gb|EYU27097.1| hypothetical protein MIMGU_mgv1a025766mg, partial... 86 7e-15
ref|XP_004300176.1| PREDICTED: disease resistance protein RPM1-l... 86 7e-15
ref|XP_004301665.1| PREDICTED: disease resistance protein RPM1-l... 84 2e-14
ref|XP_004301648.1| PREDICTED: disease resistance protein RPM1-l... 84 3e-14
ref|XP_007040756.1| Nbs-lrr resistance protein [Theobroma cacao]... 83 3e-14
ref|XP_004239116.1| PREDICTED: putative late blight resistance p... 83 3e-14
gb|AAC49408.1| PRF [Solanum lycopersicum] 83 3e-14
ref|XP_003534302.1| PREDICTED: disease resistance protein RPM1-l... 83 3e-14
gb|AAF76312.1|AF220603_4 Prf [Solanum lycopersicum] 83 3e-14
gb|AAF76308.1| Prf [Solanum pimpinellifolium] 83 3e-14
emb|CAN75824.1| hypothetical protein VITISV_004157 [Vitis vinifera] 83 4e-14
gb|EXC26229.1| Disease resistance protein RPM1 [Morus notabilis] 82 6e-14
ref|XP_003612691.1| Disease resistance protein RPP8 [Medicago tr... 82 8e-14
>ref|XP_004229601.1| PREDICTED: disease resistance protein RPM1-like [Solanum
lycopersicum]
Length = 909
Score = 92.4 bits (228), Expect = 6e-17
Identities = 51/116 (43%), Positives = 68/116 (58%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
S+E G + E++PL +ESW LF + F G SCP LE + + C GLPLAIV VGG
Sbjct: 279 SIETNGYVYEVKPLSTEESWILFCQKAFHGFSCPSHLESISRNILKKCGGLPLAIVAVGG 338
Query: 155 VLMDAGQ---KDWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL + ++W + SLG DCND +E + V+LLS+ LP Y K C LY+
Sbjct: 339 VLATKNRNNIREWGMLNHSLGPELDCNDKFESM-RIVLLLSFNDLPYYLKPCFLYL 393
>ref|XP_007210392.1| hypothetical protein PRUPE_ppa000961mg [Prunus persica]
gi|462406127|gb|EMJ11591.1| hypothetical protein
PRUPE_ppa000961mg [Prunus persica]
Length = 949
Score = 89.7 bits (221), Expect = 4e-16
Identities = 49/116 (42%), Positives = 67/116 (57%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
SVE GG + + PL + ESW L + F+G+SCP LEE C+ C GLPLAIV V G
Sbjct: 319 SVESGGKVYNMEPLPQLESWELLCKKTFQGSSCPPYLEEIGNCILRKCEGLPLAIVAVSG 378
Query: 155 VLMDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL ++ +W + SLG + ND ++L V+ LS+ LP Y K+C LY+
Sbjct: 379 VLATKDKRRIDEWDMVGHSLGAEIEGNDKLKDL-KKVLSLSFNDLPYYLKSCFLYL 433
>ref|XP_006368520.1| hypothetical protein POPTR_0001s03710g [Populus trichocarpa]
gi|550346442|gb|ERP65089.1| hypothetical protein
POPTR_0001s03710g [Populus trichocarpa]
Length = 617
Score = 89.4 bits (220), Expect = 5e-16
Identities = 46/114 (40%), Positives = 68/114 (59%), Gaps = 5/114 (4%)
Frame = -1
Query: 332 VEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGV 153
+E G + + PL+ +ESW+LF R FRGNSCP LE+ + C GLPLAIV + GV
Sbjct: 318 IESEGKVYNLEPLLPEESWTLFCRKTFRGNSCPHHLEDICKNILRKCEGLPLAIVAISGV 377
Query: 152 LMDAGQK---DWKIAASSLGDCNDDYEELID--AVMLLSYKRLPQYAKACLLYM 6
L ++ +W++ SLG +D +L++ V+ LS+ LP Y K+C LY+
Sbjct: 378 LAAKDKRRIDEWEMVRRSLGAEIEDNNKLLNLKKVLSLSFNDLPYYLKSCFLYV 431
>ref|XP_006368330.1| hypothetical protein POPTR_0001s01670g [Populus trichocarpa]
gi|550346235|gb|ERP64899.1| hypothetical protein
POPTR_0001s01670g [Populus trichocarpa]
Length = 686
Score = 89.4 bits (220), Expect = 5e-16
Identities = 46/114 (40%), Positives = 68/114 (59%), Gaps = 5/114 (4%)
Frame = -1
Query: 332 VEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGV 153
+E G + + PL+ +ESW+LF R FRGNSCP LE+ + C GLPLAIV + GV
Sbjct: 282 IESEGKVYNLEPLLPEESWTLFCRKTFRGNSCPHHLEDICKNILRKCEGLPLAIVAISGV 341
Query: 152 LMDAGQK---DWKIAASSLGDCNDDYEELID--AVMLLSYKRLPQYAKACLLYM 6
L ++ +W++ SLG +D +L++ V+ LS+ LP Y K+C LY+
Sbjct: 342 LAAKDKRRIDEWEMVRRSLGAEIEDNNKLLNLKKVLSLSFNDLPYYLKSCFLYV 395
>ref|XP_006366513.1| PREDICTED: disease resistance protein RPM1-like [Solanum tuberosum]
Length = 949
Score = 88.6 bits (218), Expect = 8e-16
Identities = 49/116 (42%), Positives = 67/116 (57%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
S+E G + E++PL +ESW LF + F G SCP LE + + C GLPLA+V VGG
Sbjct: 319 SIETNGYVYEVKPLSTEESWILFCQKAFHGYSCPSHLESISRNILKKCGGLPLAVVAVGG 378
Query: 155 VLMDAGQ---KDWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL + ++W + SLG D ND +E + V+LLS+ LP Y K C LY+
Sbjct: 379 VLATKNRNNIREWGMLNHSLGPELDSNDKFESM-RIVLLLSFNDLPYYLKPCFLYL 433
>ref|XP_003631469.1| PREDICTED: disease resistance protein RPM1-like [Vitis vinifera]
Length = 931
Score = 86.3 bits (212), Expect = 4e-15
Identities = 47/112 (41%), Positives = 66/112 (58%), Gaps = 4/112 (3%)
Frame = -1
Query: 329 EGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGVL 150
E G + ++PL ++ESW+LF + F SCP LE + C+ C GLPLAIV V GVL
Sbjct: 332 ESHGNVYTLKPLSQEESWTLFCKKTFPAESCPSYLEGISKCILQRCEGLPLAIVAVSGVL 391
Query: 149 MDA-GQKDWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
G +W+ SLG + N+ ++ L + ++LLSY LP Y K+C LYM
Sbjct: 392 STKDGIDEWESVYRSLGAELEGNNKFDSLKE-ILLLSYNDLPYYLKSCFLYM 442
>emb|CAN74463.1| hypothetical protein VITISV_032893 [Vitis vinifera]
Length = 931
Score = 86.3 bits (212), Expect = 4e-15
Identities = 47/112 (41%), Positives = 66/112 (58%), Gaps = 4/112 (3%)
Frame = -1
Query: 329 EGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGVL 150
E G + ++PL ++ESW+LF + F SCP LE + C+ C GLPLAIV V GVL
Sbjct: 332 ESHGNVYTLKPLSQEESWTLFCKKTFPAESCPSYLEGISKCILQRCEGLPLAIVAVSGVL 391
Query: 149 MDA-GQKDWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
G +W+ SLG + N+ ++ L + ++LLSY LP Y K+C LYM
Sbjct: 392 STKDGIDEWESVYRSLGAELEGNNKFDSLKE-ILLLSYNDLPYYLKSCFLYM 442
>gb|EYU27097.1| hypothetical protein MIMGU_mgv1a025766mg, partial [Mimulus
guttatus]
Length = 938
Score = 85.5 bits (210), Expect = 7e-15
Identities = 46/116 (39%), Positives = 66/116 (56%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
SVE G + ++ PL E+ESW LF R F+ NSCP L + + + C GLPLAIV + G
Sbjct: 330 SVENNGYVYKLNPLSEEESWILFCRKAFQSNSCPNHLTDICLNILRRCGGLPLAIVAISG 389
Query: 155 VL---MDAGQKDWKIAASSLGD---CNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL + +WK+ + LG ND E + + ++LLS+ LP Y K C +Y+
Sbjct: 390 VLSTKKETNIDEWKMLNNGLGSELGSNDQLESMRN-ILLLSFNYLPYYLKPCFMYL 444
>ref|XP_004300176.1| PREDICTED: disease resistance protein RPM1-like [Fragaria vesca
subsp. vesca]
Length = 924
Score = 85.5 bits (210), Expect = 7e-15
Identities = 48/116 (41%), Positives = 62/116 (53%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
SVE GG + + PL + ESW L + F+G SCP LEE C G+PLAIV V G
Sbjct: 320 SVESGGKVYNLEPLPQSESWELLCKKTFQGKSCPPHLEEICTYALKKCEGMPLAIVAVSG 379
Query: 155 VLMDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL Q+ +W + SLG ND +L V+ LS+ LP Y K+C LY+
Sbjct: 380 VLSTKDQRRIDEWDMVCHSLGAEIQGNDKLRDL-KKVLSLSFNDLPYYLKSCFLYL 434
>ref|XP_004301665.1| PREDICTED: disease resistance protein RPM1-like [Fragaria vesca
subsp. vesca]
Length = 920
Score = 84.3 bits (207), Expect = 2e-14
Identities = 47/116 (40%), Positives = 65/116 (56%), Gaps = 6/116 (5%)
Frame = -1
Query: 335 SVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGG 156
S+E GG + + PL ESW L + F+GNSCP LE+ V C GLPLAIV + G
Sbjct: 313 SMESGGEVYTLEPLPPMESWELLCKKTFQGNSCPPHLEKICNYVLKKCEGLPLAIVAISG 372
Query: 155 VLMDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
VL ++ +W + SLG + ND ++L V+ LS+ LP Y K+C LY+
Sbjct: 373 VLAAKDKRRIDEWDMVGHSLGYEIEGNDKLQDL-KKVLSLSFNDLPYYLKSCFLYL 427
>ref|XP_004301648.1| PREDICTED: disease resistance protein RPM1-like [Fragaria vesca
subsp. vesca]
Length = 954
Score = 83.6 bits (205), Expect = 3e-14
Identities = 50/112 (44%), Positives = 61/112 (54%), Gaps = 6/112 (5%)
Frame = -1
Query: 329 EGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGVL 150
E GG + E+RPL EKESWSLF R F+ SCP LE + C GLPLAIV + GVL
Sbjct: 324 ESGGHVHEVRPLPEKESWSLFSRKAFQRKSCPSYLESFCEDILKKCEGLPLAIVAISGVL 383
Query: 149 MDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLL 12
++ +W SLG ND E+L V+ LS+ LP KAC L
Sbjct: 384 ATKDERRIDEWDAICRSLGAEIHGNDKLEDL-KKVLSLSFNDLPYNLKACFL 434
>ref|XP_007040756.1| Nbs-lrr resistance protein [Theobroma cacao]
gi|508778001|gb|EOY25257.1| Nbs-lrr resistance protein
[Theobroma cacao]
Length = 947
Score = 83.2 bits (204), Expect = 3e-14
Identities = 44/114 (38%), Positives = 65/114 (57%), Gaps = 5/114 (4%)
Frame = -1
Query: 332 VEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGV 153
+E G + + PL + SW+LF + FRGN CP LEE + C GLPLAIV + GV
Sbjct: 319 IESEGEVYNLEPLPPEVSWTLFCKKTFRGNYCPPYLEEICKQILKKCEGLPLAIVAISGV 378
Query: 152 LMDAGQK---DWKIAASSLGDCNDDYEELID--AVMLLSYKRLPQYAKACLLYM 6
L ++ +W++ SLG D ++L++ V+ LS+ LP Y K+C LY+
Sbjct: 379 LATKSKRRIDEWEMVGRSLGAEIDGNDKLMNLKKVLSLSFNDLPYYLKSCFLYL 432
>ref|XP_004239116.1| PREDICTED: putative late blight resistance protein homolog
R1B-17-like [Solanum lycopersicum]
Length = 1825
Score = 83.2 bits (204), Expect = 3e-14
Identities = 46/117 (39%), Positives = 63/117 (53%), Gaps = 2/117 (1%)
Frame = -1
Query: 347 EVAESVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIV 168
+VAE V+ +R + ESW+L Q+ VF+G SCP LE+ +S +CRGLPL++V
Sbjct: 1235 DVAEYVKCESDPHHLRLFRDDESWTLLQKEVFQGESCPPELEDVGFEISKSCRGLPLSVV 1294
Query: 167 TVGGVLMDAGQ--KDWKIAASSLGDCNDDYEELIDAVMLLSYKRLPQYAKACLLYMG 3
V GVL + WK+ SL E +++ SYK LP Y K C LY G
Sbjct: 1295 LVAGVLKQKKKTLDSWKVVEQSLSSQRIGSLEESISIIGFSYKNLPHYLKPCFLYFG 1351
>gb|AAC49408.1| PRF [Solanum lycopersicum]
Length = 1824
Score = 83.2 bits (204), Expect = 3e-14
Identities = 46/117 (39%), Positives = 63/117 (53%), Gaps = 2/117 (1%)
Frame = -1
Query: 347 EVAESVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIV 168
+VAE V+ +R + ESW+L Q+ VF+G SCP LE+ +S +CRGLPL++V
Sbjct: 1234 DVAEYVKCESDPHHLRLFRDDESWTLLQKEVFQGESCPPELEDVGFEISKSCRGLPLSVV 1293
Query: 167 TVGGVLMDAGQ--KDWKIAASSLGDCNDDYEELIDAVMLLSYKRLPQYAKACLLYMG 3
V GVL + WK+ SL E +++ SYK LP Y K C LY G
Sbjct: 1294 LVAGVLKQKKKTLDSWKVVEQSLSSQRIGSLEESISIIGFSYKNLPHYLKPCFLYFG 1350
>ref|XP_003534302.1| PREDICTED: disease resistance protein RPM1-like [Glycine max]
Length = 937
Score = 83.2 bits (204), Expect = 3e-14
Identities = 46/111 (41%), Positives = 66/111 (59%), Gaps = 6/111 (5%)
Frame = -1
Query: 320 GPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGVLMDA 141
G ++ L E+E+W LF + F+GNSCP LEE + C GLPLAIV +GG L
Sbjct: 311 GKDFDLEFLPEEEAWYLFCKKTFQGNSCPPHLEEVCRKILKMCGGLPLAIVGIGGALATK 370
Query: 140 GQ---KDWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
G+ ++W++ SLG + ND E++ V+ LS+ LP Y K+CLLY+
Sbjct: 371 GRANIEEWQMVCRSLGSEIEGNDKLEDM-KKVLSLSFNELPYYLKSCLLYL 420
>gb|AAF76312.1|AF220603_4 Prf [Solanum lycopersicum]
Length = 1825
Score = 83.2 bits (204), Expect = 3e-14
Identities = 46/117 (39%), Positives = 63/117 (53%), Gaps = 2/117 (1%)
Frame = -1
Query: 347 EVAESVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIV 168
+VAE V+ +R + ESW+L Q+ VF+G SCP LE+ +S +CRGLPL++V
Sbjct: 1235 DVAEYVKCESDPHHLRLFRDDESWTLLQKEVFQGESCPPELEDVGFEISKSCRGLPLSVV 1294
Query: 167 TVGGVLMDAGQ--KDWKIAASSLGDCNDDYEELIDAVMLLSYKRLPQYAKACLLYMG 3
V GVL + WK+ SL E +++ SYK LP Y K C LY G
Sbjct: 1295 LVAGVLKQKKKTLDSWKVVEQSLSSQRIGSLEESISIIGFSYKNLPHYLKPCFLYFG 1351
>gb|AAF76308.1| Prf [Solanum pimpinellifolium]
Length = 1824
Score = 83.2 bits (204), Expect = 3e-14
Identities = 46/117 (39%), Positives = 63/117 (53%), Gaps = 2/117 (1%)
Frame = -1
Query: 347 EVAESVEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIV 168
+VAE V+ +R + ESW+L Q+ VF+G SCP LE+ +S +CRGLPL++V
Sbjct: 1234 DVAEYVKCESDPHHLRLFRDDESWTLLQKEVFQGESCPPELEDVGFEISKSCRGLPLSVV 1293
Query: 167 TVGGVLMDAGQ--KDWKIAASSLGDCNDDYEELIDAVMLLSYKRLPQYAKACLLYMG 3
V GVL + WK+ SL E +++ SYK LP Y K C LY G
Sbjct: 1294 LVAGVLKQKKKTLDSWKVVEQSLSSQRIGSLEESISIIGFSYKNLPHYLKPCFLYFG 1350
>emb|CAN75824.1| hypothetical protein VITISV_004157 [Vitis vinifera]
Length = 1512
Score = 82.8 bits (203), Expect = 4e-14
Identities = 46/115 (40%), Positives = 61/115 (53%), Gaps = 6/115 (5%)
Frame = -1
Query: 332 VEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGV 153
VE G + + PL ESW+LF R FR N+CP L + + + C GLPLAIV +GGV
Sbjct: 275 VESDGDVFPLNPLPPTESWTLFCRKAFRRNACPPHLNKLSQGILKRCEGLPLAIVAIGGV 334
Query: 152 LMDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
L Q +W I SL + ND E ++ ++ L Y LP Y K C LY+
Sbjct: 335 LATKDQNRMDEWDIVDRSLSSELESNDKLER-VNKILSLGYNDLPYYLKHCFLYL 388
>gb|EXC26229.1| Disease resistance protein RPM1 [Morus notabilis]
Length = 949
Score = 82.4 bits (202), Expect = 6e-14
Identities = 46/115 (40%), Positives = 61/115 (53%), Gaps = 6/115 (5%)
Frame = -1
Query: 332 VEGGGPLLEIRPLVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGV 153
+E G + PL +SW L R F+GN CP LEE + C GLPLAIV + GV
Sbjct: 320 IESEGKTYNLEPLPPSDSWDLLCRKTFQGNPCPPHLEEICKYILKRCEGLPLAIVAISGV 379
Query: 152 LMDAGQK---DWKIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
L + +W + A SLG D ND ++L V+ LS+ LP Y K+C LY+
Sbjct: 380 LATKDTRRIDEWDMVARSLGAEIDGNDKLKDL-KKVLSLSFNDLPYYLKSCFLYL 433
>ref|XP_003612691.1| Disease resistance protein RPP8 [Medicago truncatula]
gi|355514026|gb|AES95649.1| Disease resistance protein
RPP8 [Medicago truncatula]
Length = 944
Score = 82.0 bits (201), Expect = 8e-14
Identities = 45/103 (43%), Positives = 62/103 (60%), Gaps = 6/103 (5%)
Frame = -1
Query: 296 LVEKESWSLFQRVVFRGNSCPEGLEETAMCVSANCRGLPLAIVTVGGVLMDAGQ---KDW 126
L E+E+WSLF R F+GNSCP LEE + C GLPLAIV + G L G+ ++W
Sbjct: 322 LPEQEAWSLFCRKTFQGNSCPPHLEEVCRNILKLCGGLPLAIVAISGALATRGRTNIEEW 381
Query: 125 KIAASSLG---DCNDDYEELIDAVMLLSYKRLPQYAKACLLYM 6
+I S G + ND E++ V+ LS+ LP + K+CLLY+
Sbjct: 382 QIVCRSFGSEIEGNDKLEDM-KKVLSLSFNELPYHLKSCLLYL 423