BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Mentha23_contig00020935
         (313 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EYU38248.1| hypothetical protein MIMGU_mgv1a006691mg [Mimulus...   115   8e-24
gb|EYU38247.1| hypothetical protein MIMGU_mgv1a006691mg [Mimulus...   115   8e-24
ref|XP_007219455.1| hypothetical protein PRUPE_ppa021176mg [Prun...    97   2e-18
gb|EXC10465.1| Protein IQ-DOMAIN 14 [Morus notabilis]                  96   4e-18
gb|EPS69586.1| hypothetical protein M569_05181, partial [Genlise...    94   1e-17
ref|XP_003605876.1| IQ domain-containing protein [Medicago trunc...    92   7e-17
ref|XP_004303073.1| PREDICTED: protein IQ-DOMAIN 14-like [Fragar...    92   1e-16
ref|XP_006587682.1| PREDICTED: protein IQ-DOMAIN 14-like isoform...    89   5e-16
ref|XP_003534374.1| PREDICTED: protein IQ-DOMAIN 14-like isoform...    89   5e-16
ref|XP_007149021.1| hypothetical protein PHAVU_005G033800g [Phas...    86   4e-15
ref|XP_002513117.1| conserved hypothetical protein [Ricinus comm...    86   4e-15
ref|XP_004513265.1| PREDICTED: protein IQ-DOMAIN 14-like isoform...    84   2e-14
ref|XP_004513264.1| PREDICTED: protein IQ-DOMAIN 14-like isoform...    84   2e-14
ref|XP_003539928.1| PREDICTED: protein IQ-DOMAIN 14-like [Glycin...    84   2e-14
ref|XP_004515017.1| PREDICTED: protein IQ-DOMAIN 14-like [Cicer ...    82   6e-14
ref|XP_006345487.1| PREDICTED: protein IQ-DOMAIN 14-like [Solanu...    82   8e-14
ref|XP_004229688.1| PREDICTED: protein IQ-DOMAIN 14-like [Solanu...    82   1e-13
ref|XP_007131445.1| hypothetical protein PHAVU_011G014100g [Phas...    80   3e-13
ref|XP_006368583.1| calmodulin-binding family protein [Populus t...    80   4e-13
emb|CBI17753.3| unnamed protein product [Vitis vinifera]               78   1e-12

>gb|EYU38248.1| hypothetical protein MIMGU_mgv1a006691mg [Mimulus guttatus]
          Length = 434

 Score =  115 bits (287), Expect = 8e-24
 Identities = 59/104 (56%), Positives = 68/104 (65%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           DLNSQK WDDS                  IKRER +EYYL+HRRSAETERDK YGRRRYW
Sbjct: 196 DLNSQKRWDDSTLTKEELKALLLTKRDASIKRERVKEYYLNHRRSAETERDKAYGRRRYW 255

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGRLRNFQKQYQ 312
           LEQWVD+QLAKS++P   +  LSAN RN +     +R+  KQ Q
Sbjct: 256 LEQWVDAQLAKSDTPRNLDRNLSANDRNRE-----IRHLPKQNQ 294


>gb|EYU38247.1| hypothetical protein MIMGU_mgv1a006691mg [Mimulus guttatus]
          Length = 435

 Score =  115 bits (287), Expect = 8e-24
 Identities = 59/104 (56%), Positives = 68/104 (65%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           DLNSQK WDDS                  IKRER +EYYL+HRRSAETERDK YGRRRYW
Sbjct: 197 DLNSQKRWDDSTLTKEELKALLLTKRDASIKRERVKEYYLNHRRSAETERDKAYGRRRYW 256

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGRLRNFQKQYQ 312
           LEQWVD+QLAKS++P   +  LSAN RN +     +R+  KQ Q
Sbjct: 257 LEQWVDAQLAKSDTPRNLDRNLSANDRNRE-----IRHLPKQNQ 295


>ref|XP_007219455.1| hypothetical protein PRUPE_ppa021176mg [Prunus persica]
           gi|462415917|gb|EMJ20654.1| hypothetical protein
           PRUPE_ppa021176mg [Prunus persica]
          Length = 459

 Score = 97.4 bits (241), Expect = 2e-18
 Identities = 50/105 (47%), Positives = 65/105 (61%), Gaps = 3/105 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NSQ+ WD S                  +KRER REY  +HR+SAE+ER+KV GR RYW
Sbjct: 217 DSNSQRRWDGSLISKEEAEALFLSKKEAMLKRERIREYSYTHRKSAESERNKVNGRWRYW 276

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGG---RLRNFQKQ 306
           L+QWVD+QL+KS+     +T L  N++  +E GG   RLRNFQ+Q
Sbjct: 277 LDQWVDTQLSKSKELEDLDTVLDLNAKRKEEFGGKQLRLRNFQRQ 321


>gb|EXC10465.1| Protein IQ-DOMAIN 14 [Morus notabilis]
          Length = 469

 Score = 96.3 bits (238), Expect = 4e-18
 Identities = 52/107 (48%), Positives = 66/107 (61%), Gaps = 3/107 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D  +Q+ WDDS                  IKRER +EY+ SHR+SAE+ER+KV GR RYW
Sbjct: 226 DSFNQRRWDDSLLSKEEADAMFLSKKEAAIKRERIKEYWHSHRKSAESERNKVNGRWRYW 285

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR---LRNFQKQYQ 312
           LEQWVD+QL KS+     +T L +N+RN DE G +   LRN Q Q+Q
Sbjct: 286 LEQWVDTQLNKSKELEDLDTVLGSNTRNKDEFGVKKLGLRNVQTQHQ 332


>gb|EPS69586.1| hypothetical protein M569_05181, partial [Genlisea aurea]
          Length = 313

 Score = 94.4 bits (233), Expect = 1e-17
 Identities = 51/107 (47%), Positives = 68/107 (63%), Gaps = 3/107 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           DLNSQ+ WD+S                  +K+ER +EYY+SHRRSAETER++V+ R+RYW
Sbjct: 99  DLNSQQNWDNSTLTKEEMKALVLAKRESSLKKERIQEYYISHRRSAETERNRVHERQRYW 158

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR---LRNFQKQYQ 312
           L+QWVD  L+K+E+  T NT     +RN DE G R   LR +Q+Q Q
Sbjct: 159 LKQWVDIHLSKNEA--TVNTE-RVKTRNRDEAGNRVPNLRQYQRQQQ 202


>ref|XP_003605876.1| IQ domain-containing protein [Medicago truncatula]
           gi|358344582|ref|XP_003636367.1| IQ domain-containing
           protein [Medicago truncatula]
           gi|355502302|gb|AES83505.1| IQ domain-containing protein
           [Medicago truncatula] gi|355506931|gb|AES88073.1| IQ
           domain-containing protein [Medicago truncatula]
          Length = 436

 Score = 92.0 bits (227), Expect = 7e-17
 Identities = 49/105 (46%), Positives = 64/105 (60%), Gaps = 3/105 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WDDS                  IKRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 190 DSNSERKWDDSTLLKEEVDASCMIKKEGIIKRERIKEYTFNHRRSAESERSKVNGRWRYW 249

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR---LRNFQKQ 306
           LEQWVD+QL+KS+     ++  S++S+  DE GGR   LR+  +Q
Sbjct: 250 LEQWVDTQLSKSKELEDLDSVYSSHSKIGDEFGGRQLKLRSTNRQ 294


>ref|XP_004303073.1| PREDICTED: protein IQ-DOMAIN 14-like [Fragaria vesca subsp. vesca]
          Length = 454

 Score = 91.7 bits (226), Expect = 1e-16
 Identities = 49/106 (46%), Positives = 64/106 (60%), Gaps = 4/106 (3%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NSQ+ WDD                   IKRER +EY+ SHR+SAE+ER KV GR RYW
Sbjct: 209 DSNSQRRWDDRLVSKEEAEALCLSKKEAMIKRERIKEYWYSHRKSAESERKKVNGRWRYW 268

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGG----RLRNFQKQ 306
           L+QWVD+Q++KS+     +T L+A  +  +E GG    RLRN Q+Q
Sbjct: 269 LDQWVDTQISKSKELEDLDTVLAAKRK--EEFGGGKQDRLRNLQRQ 312


>ref|XP_006587682.1| PREDICTED: protein IQ-DOMAIN 14-like isoform X2 [Glycine max]
           gi|571478838|ref|XP_006587683.1| PREDICTED: protein
           IQ-DOMAIN 14-like isoform X3 [Glycine max]
           gi|571478840|ref|XP_006587684.1| PREDICTED: protein
           IQ-DOMAIN 14-like isoform X4 [Glycine max]
          Length = 469

 Score = 89.4 bits (220), Expect = 5e-16
 Identities = 44/95 (46%), Positives = 59/95 (62%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WD+S                  +KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 218 DSNSERKWDESTVLKEEVDTSCTSKKETILKRERIKEYSFNHRRSAESERSKVNGRWRYW 277

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR 285
           LEQWVD+QL+KS+     ++  S++SR  +E GGR
Sbjct: 278 LEQWVDTQLSKSKELEDLDSVFSSHSRAGEEYGGR 312


>ref|XP_003534374.1| PREDICTED: protein IQ-DOMAIN 14-like isoform X1 [Glycine max]
          Length = 468

 Score = 89.4 bits (220), Expect = 5e-16
 Identities = 44/95 (46%), Positives = 59/95 (62%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WD+S                  +KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 217 DSNSERKWDESTVLKEEVDTSCTSKKETILKRERIKEYSFNHRRSAESERSKVNGRWRYW 276

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR 285
           LEQWVD+QL+KS+     ++  S++SR  +E GGR
Sbjct: 277 LEQWVDTQLSKSKELEDLDSVFSSHSRAGEEYGGR 311


>ref|XP_007149021.1| hypothetical protein PHAVU_005G033800g [Phaseolus vulgaris]
           gi|561022285|gb|ESW21015.1| hypothetical protein
           PHAVU_005G033800g [Phaseolus vulgaris]
          Length = 450

 Score = 86.3 bits (212), Expect = 4e-15
 Identities = 48/108 (44%), Positives = 64/108 (59%), Gaps = 4/108 (3%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WDDS                  +KRER +EY  +HR+SAE+ERDK+ GR RYW
Sbjct: 218 DSNSERRWDDSMLLKEEVDASCMSKKEAVVKRERVKEYSFNHRKSAESERDKINGRWRYW 277

Query: 181 LEQWVDSQLAKSESPGTFNTTLSA-NSRNIDEIGGR---LRNFQKQYQ 312
           +EQWVD+QL+KS+     ++  S+ +SR   E G R   LRN Q Q Q
Sbjct: 278 MEQWVDTQLSKSKELEDLDSVFSSHHSRTGKESGKRQLKLRNTQIQNQ 325


>ref|XP_002513117.1| conserved hypothetical protein [Ricinus communis]
           gi|223548128|gb|EEF49620.1| conserved hypothetical
           protein [Ricinus communis]
          Length = 510

 Score = 86.3 bits (212), Expect = 4e-15
 Identities = 47/106 (44%), Positives = 61/106 (57%), Gaps = 3/106 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NSQ+ WD S                  IKRER REY  +HR SAE+ER+KV GR RYW
Sbjct: 270 DTNSQRRWDGSIFTKEEAEALFLSKKDAAIKRERIREYAFNHRNSAESERNKVNGRWRYW 329

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGG---RLRNFQKQY 309
           LEQWVD+Q++KS+     +T L++  +   E  G   +LR  Q+QY
Sbjct: 330 LEQWVDTQVSKSKELEDLDTVLTSTPKPRVEYRGKQLKLRGLQRQY 375


>ref|XP_004513265.1| PREDICTED: protein IQ-DOMAIN 14-like isoform X2 [Cicer arietinum]
          Length = 446

 Score = 84.3 bits (207), Expect = 2e-14
 Identities = 41/87 (47%), Positives = 54/87 (62%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WDDS                  +KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 199 DSNSERKWDDSTLLKEEVDAFCMIKKEAILKRERIKEYTFNHRRSAESERSKVNGRWRYW 258

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSR 261
           LEQWVD+QL+KS+     ++  S++SR
Sbjct: 259 LEQWVDTQLSKSKELEDLDSVFSSHSR 285


>ref|XP_004513264.1| PREDICTED: protein IQ-DOMAIN 14-like isoform X1 [Cicer arietinum]
          Length = 447

 Score = 84.3 bits (207), Expect = 2e-14
 Identities = 41/87 (47%), Positives = 54/87 (62%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WDDS                  +KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 200 DSNSERKWDDSTLLKEEVDAFCMIKKEAILKRERIKEYTFNHRRSAESERSKVNGRWRYW 259

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSR 261
           LEQWVD+QL+KS+     ++  S++SR
Sbjct: 260 LEQWVDTQLSKSKELEDLDSVFSSHSR 286


>ref|XP_003539928.1| PREDICTED: protein IQ-DOMAIN 14-like [Glycine max]
          Length = 460

 Score = 84.3 bits (207), Expect = 2e-14
 Identities = 49/106 (46%), Positives = 64/106 (60%), Gaps = 4/106 (3%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WD+S                   KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 217 DSNSERKWDESTVLKEEVDTSCTS------KRERTKEYSFNHRRSAESERSKVNGRWRYW 270

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR---LR-NFQKQ 306
           LEQWVD+QL+KS+     ++  S++SR  +E GGR   LR N Q+Q
Sbjct: 271 LEQWVDTQLSKSKELEDLDSVFSSHSRAGEEYGGRQLKLRSNIQRQ 316


>ref|XP_004515017.1| PREDICTED: protein IQ-DOMAIN 14-like [Cicer arietinum]
          Length = 411

 Score = 82.4 bits (202), Expect = 6e-14
 Identities = 40/105 (38%), Positives = 63/105 (60%), Gaps = 3/105 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++ WDDS                  ++RER +EY  +HR+SAE ER+K+ GR RYW
Sbjct: 211 DSNSERRWDDSLLLKKEVDSSSISKKEASLRRERVKEYSYNHRKSAEPERNKINGRWRYW 270

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR---LRNFQKQ 306
           +EQWVD+Q +KS+     ++  +++ R +++ G R   LR+ Q+Q
Sbjct: 271 MEQWVDTQHSKSKELEDLDSEFTSHCRGVEDYGRRQVKLRHIQRQ 315


>ref|XP_006345487.1| PREDICTED: protein IQ-DOMAIN 14-like [Solanum tuberosum]
          Length = 430

 Score = 82.0 bits (201), Expect = 8e-14
 Identities = 44/87 (50%), Positives = 52/87 (59%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           DLNSQK WD+                   IKRER REY+LSHRRS E++   V GRRRYW
Sbjct: 222 DLNSQKRWDNRYLSKEEANKMFSSKREAAIKRERIREYWLSHRRSTESD---VNGRRRYW 278

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSR 261
           LEQWVD+QLAK +     +T  SA +R
Sbjct: 279 LEQWVDAQLAKRDDLKNVDTLFSARNR 305


>ref|XP_004229688.1| PREDICTED: protein IQ-DOMAIN 14-like [Solanum lycopersicum]
          Length = 458

 Score = 81.6 bits (200), Expect = 1e-13
 Identities = 43/87 (49%), Positives = 52/87 (59%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           DLNSQK WD+                   IKRER REY+LSHRRS E++   + GRRRYW
Sbjct: 216 DLNSQKRWDNRYLSKEEANKMFSSKREAAIKRERIREYWLSHRRSTESD---INGRRRYW 272

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSR 261
           LEQWVD+QLAK +     +T  SA +R
Sbjct: 273 LEQWVDAQLAKRDDLKNVDTLFSARNR 299


>ref|XP_007131445.1| hypothetical protein PHAVU_011G014100g [Phaseolus vulgaris]
           gi|593101560|ref|XP_007131446.1| hypothetical protein
           PHAVU_011G014100g [Phaseolus vulgaris]
           gi|593101656|ref|XP_007131447.1| hypothetical protein
           PHAVU_011G014100g [Phaseolus vulgaris]
           gi|561004445|gb|ESW03439.1| hypothetical protein
           PHAVU_011G014100g [Phaseolus vulgaris]
           gi|561004446|gb|ESW03440.1| hypothetical protein
           PHAVU_011G014100g [Phaseolus vulgaris]
           gi|561004447|gb|ESW03441.1| hypothetical protein
           PHAVU_011G014100g [Phaseolus vulgaris]
          Length = 468

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 41/95 (43%), Positives = 56/95 (58%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D NS++  DDS                  +KRER +EY  +HRRSAE+ER KV GR RYW
Sbjct: 222 DSNSERKCDDSTPSKEELDTSCISMKETVLKRERIKEYSFNHRRSAESERSKVNGRWRYW 281

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGR 285
           LEQWVD+QL+KS+     ++  S+++   +E G R
Sbjct: 282 LEQWVDTQLSKSKELEDLDSVFSSHAGGGEEYGSR 316


>ref|XP_006368583.1| calmodulin-binding family protein [Populus trichocarpa]
           gi|550346599|gb|ERP65152.1| calmodulin-binding family
           protein [Populus trichocarpa]
          Length = 464

 Score = 79.7 bits (195), Expect = 4e-13
 Identities = 43/106 (40%), Positives = 59/106 (55%), Gaps = 3/106 (2%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHRRSAETERDKVYGRRRYW 180
           D+NS++ WD S                  IKRER REY+ + R SAE+ER K  GR RYW
Sbjct: 219 DMNSERRWDSSLLTKEEAVASFLSKKEAAIKRERIREYWFNRRNSAESERSKPSGRWRYW 278

Query: 181 LEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGG---RLRNFQKQY 309
           L+QWVD+QL KS+     ++ L++N +   E  G   +LR  Q+ Y
Sbjct: 279 LDQWVDTQLVKSKELEDLDSVLTSNPKPGVEYRGKQIKLRGLQRLY 324


>emb|CBI17753.3| unnamed protein product [Vitis vinifera]
          Length = 354

 Score = 78.2 bits (191), Expect = 1e-12
 Identities = 44/112 (39%), Positives = 63/112 (56%), Gaps = 9/112 (8%)
 Frame = +1

Query: 1   DLNSQKGWDDSXXXXXXXXXXXXXXXXXFIKRERAREYYLSHR------RSAETERDKVY 162
           D NSQ+ WDDS                  +KRER +EY    R      + AE+E++K+ 
Sbjct: 223 DSNSQRRWDDSLLSKEEGNALFLSKKEAVMKRERIKEYTFGQRERKSIHKPAESEQNKLN 282

Query: 163 GRRRYWLEQWVDSQLAKSESPGTFNTTLSANSRNIDEIGGRL---RNFQKQY 309
           GR RYWLE+WVD+Q+AK E   + +T  S+N+R+ +E  G+    RN Q+QY
Sbjct: 283 GRWRYWLEKWVDTQVAKREELPSLDTVWSSNARSREEFPGKQHTPRNNQRQY 334