BLASTX nr result
ID: Mentha23_contig00016799
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha23_contig00016799
(314 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_007044230.1| Leucine-rich repeat protein kinase family pr... 185 5e-45
ref|XP_002282768.1| PREDICTED: probable inactive leucine-rich re... 182 5e-44
emb|CAN73490.1| hypothetical protein VITISV_040575 [Vitis vinifera] 182 5e-44
ref|XP_007227346.1| hypothetical protein PRUPE_ppa002204mg [Prun... 179 3e-43
ref|XP_004299037.1| PREDICTED: probable inactive leucine-rich re... 176 4e-42
ref|XP_002526283.1| ATP binding protein, putative [Ricinus commu... 175 5e-42
ref|XP_002315920.1| leucine-rich repeat transmembrane protein ki... 175 7e-42
gb|EYU40794.1| hypothetical protein MIMGU_mgv1a024372mg, partial... 173 2e-41
ref|XP_006344171.1| PREDICTED: probable inactive leucine-rich re... 173 3e-41
ref|XP_004238893.1| PREDICTED: probable inactive leucine-rich re... 171 1e-40
ref|XP_006468755.1| PREDICTED: probable inactive leucine-rich re... 167 1e-39
ref|XP_006448399.1| hypothetical protein CICLE_v10014433mg [Citr... 167 1e-39
ref|XP_004164156.1| PREDICTED: probable inactive leucine-rich re... 167 1e-39
ref|XP_004135080.1| PREDICTED: probable inactive leucine-rich re... 167 1e-39
ref|XP_004161994.1| PREDICTED: probable inactive leucine-rich re... 165 5e-39
ref|XP_004147142.1| PREDICTED: probable inactive leucine-rich re... 165 5e-39
ref|XP_002311473.2| leucine-rich repeat transmembrane protein ki... 161 8e-38
ref|XP_004497046.1| PREDICTED: probable inactive leucine-rich re... 161 1e-37
ref|XP_003555049.1| PREDICTED: probable inactive leucine-rich re... 159 4e-37
ref|XP_006290656.1| hypothetical protein CARUB_v10016748mg [Caps... 157 2e-36
>ref|XP_007044230.1| Leucine-rich repeat protein kinase family protein [Theobroma cacao]
gi|508708165|gb|EOY00062.1| Leucine-rich repeat protein
kinase family protein [Theobroma cacao]
Length = 716
Score = 185 bits (470), Expect = 5e-45
Identities = 91/103 (88%), Positives = 97/103 (94%)
Frame = +2
Query: 5 SKSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
SK KD +GYGFEK KG K+CLCFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASA
Sbjct: 351 SKDKDDNGYGFEKGGKGKKDCLCFRKDESETLSENVEQYDLVPLDTQVAFDLDELLKASA 410
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLE+G+TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 411 FVLGKSGIGIVYKVVLEDGLTLAVRRLGEGGSQRFKEFQTEVE 453
>ref|XP_002282768.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830 [Vitis vinifera]
gi|297737773|emb|CBI26974.3| unnamed protein product
[Vitis vinifera]
Length = 713
Score = 182 bits (461), Expect = 5e-44
Identities = 90/102 (88%), Positives = 95/102 (93%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K KD++GYGFEK K KECLCFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASAF
Sbjct: 349 KGKDENGYGFEKGGKARKECLCFRKDESETLSENVEQYDLVPLDTQVAFDLDELLKASAF 408
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 409 VLGKSGIGIVYKVVLEDGSTLAVRRLGEGGSQRFKEFQTEVE 450
>emb|CAN73490.1| hypothetical protein VITISV_040575 [Vitis vinifera]
Length = 713
Score = 182 bits (461), Expect = 5e-44
Identities = 90/102 (88%), Positives = 95/102 (93%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K KD++GYGFEK K KECLCFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASAF
Sbjct: 349 KGKDENGYGFEKGGKARKECLCFRKDESETLSENVEQYDLVPLDTQVAFDLDELLKASAF 408
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 409 VLGKSGIGIVYKVVLEDGSTLAVRRLGEGGSQRFKEFQTEVE 450
>ref|XP_007227346.1| hypothetical protein PRUPE_ppa002204mg [Prunus persica]
gi|462424282|gb|EMJ28545.1| hypothetical protein
PRUPE_ppa002204mg [Prunus persica]
Length = 701
Score = 179 bits (455), Expect = 3e-43
Identities = 89/103 (86%), Positives = 96/103 (93%)
Frame = +2
Query: 5 SKSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
SK KD++GYG +K KG KECLCFR+DESETLSEN+E YDLV LD+QVAFDLDELLKASA
Sbjct: 351 SKVKDENGYGIDKGGKGRKECLCFRKDESETLSENMEQYDLVALDAQVAFDLDELLKASA 410
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLEEG+TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 411 FVLGKSGIGIVYKVVLEEGLTLAVRRLGEGGSQRFKEFQTEVE 453
>ref|XP_004299037.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Fragaria vesca subsp.
vesca]
Length = 714
Score = 176 bits (445), Expect = 4e-42
Identities = 88/103 (85%), Positives = 94/103 (91%)
Frame = +2
Query: 5 SKSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
SK KD++GYG K KG KECLCFR+DESETLSE +E YDLV LD+QVAFDLDELLKASA
Sbjct: 348 SKVKDENGYGVAKGGKGRKECLCFRKDESETLSETMEQYDLVALDTQVAFDLDELLKASA 407
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLEEG+TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 408 FVLGKSGIGIVYKVVLEEGLTLAVRRLGEGGSQRFKEFQTEVE 450
>ref|XP_002526283.1| ATP binding protein, putative [Ricinus communis]
gi|223534364|gb|EEF36072.1| ATP binding protein,
putative [Ricinus communis]
Length = 715
Score = 175 bits (444), Expect = 5e-42
Identities = 87/102 (85%), Positives = 94/102 (92%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K KD+S Y F+K KG KECLCFR+DESETLSE+VE YDLVPLD+QV FDLDELLKASAF
Sbjct: 351 KDKDESDYVFDKRGKGRKECLCFRKDESETLSEHVEQYDLVPLDTQVTFDLDELLKASAF 410
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G+TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 411 VLGKSGIGIVYKVVLEDGLTLAVRRLGEGGSQRFKEFQTEVE 452
>ref|XP_002315920.1| leucine-rich repeat transmembrane protein kinase [Populus
trichocarpa] gi|222864960|gb|EEF02091.1| leucine-rich
repeat transmembrane protein kinase [Populus
trichocarpa]
Length = 716
Score = 175 bits (443), Expect = 7e-42
Identities = 87/103 (84%), Positives = 93/103 (90%)
Frame = +2
Query: 5 SKSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
SK +D + YGFEK K +EC CFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASA
Sbjct: 351 SKDRDGNSYGFEKGGKKRRECFCFRKDESETLSENVEQYDLVPLDAQVAFDLDELLKASA 410
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 411 FVLGKSGIGIVYKVVLEDGHTLAVRRLGEGGSQRFKEFQTEVE 453
>gb|EYU40794.1| hypothetical protein MIMGU_mgv1a024372mg, partial [Mimulus
guttatus]
Length = 636
Score = 173 bits (439), Expect = 2e-41
Identities = 86/104 (82%), Positives = 93/104 (89%)
Frame = +2
Query: 2 GSKSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKAS 181
G K KD++ YGF K SKG KECLCF +DESETLSENVE Y LVPLD+ +AFDLDELLKAS
Sbjct: 349 GIKRKDENAYGFGKGSKGRKECLCFTKDESETLSENVEQYGLVPLDTHIAFDLDELLKAS 408
Query: 182 AFVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
AFVLGKSGIGIVYKVVLE+G+TLAVRRLGEGGSQR KEFQTEVE
Sbjct: 409 AFVLGKSGIGIVYKVVLEDGLTLAVRRLGEGGSQRLKEFQTEVE 452
>ref|XP_006344171.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Solanum tuberosum]
Length = 714
Score = 173 bits (438), Expect = 3e-41
Identities = 87/103 (84%), Positives = 95/103 (92%), Gaps = 1/103 (0%)
Frame = +2
Query: 8 KSKDKSGYGFEKAS-KGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
+ KD+SG+GF+K KG KECLCFR+DESETLSENVE YDLV LD+QVAFDLDELLKASA
Sbjct: 349 RKKDESGFGFQKGGGKGRKECLCFRKDESETLSENVEQYDLVALDNQVAFDLDELLKASA 408
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLE+G+ LAVRRLGEGGSQRFKEFQTEVE
Sbjct: 409 FVLGKSGIGIVYKVVLEDGLNLAVRRLGEGGSQRFKEFQTEVE 451
>ref|XP_004238893.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Solanum lycopersicum]
Length = 715
Score = 171 bits (433), Expect = 1e-40
Identities = 86/103 (83%), Positives = 94/103 (91%), Gaps = 1/103 (0%)
Frame = +2
Query: 8 KSKDKSGYGFEKAS-KGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASA 184
+ KD+ G+GF+K KG KECLCFR+DESETLSENVE YDLV LD+QVAFDLDELLKASA
Sbjct: 350 RKKDEPGFGFQKGGGKGRKECLCFRKDESETLSENVEQYDLVALDNQVAFDLDELLKASA 409
Query: 185 FVLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
FVLGKSGIGIVYKVVLE+G+ LAVRRLGEGGSQRFKEFQTEVE
Sbjct: 410 FVLGKSGIGIVYKVVLEDGLNLAVRRLGEGGSQRFKEFQTEVE 452
>ref|XP_006468755.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Citrus sinensis]
Length = 714
Score = 167 bits (423), Expect = 1e-39
Identities = 86/102 (84%), Positives = 92/102 (90%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
+ KD++ Y K KG KECLCFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASAF
Sbjct: 352 EGKDENCYA--KGGKGRKECLCFRKDESETLSENVEQYDLVPLDTQVAFDLDELLKASAF 409
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 410 VLGKSGIGIVYKVVLEDGHTLAVRRLGEGGSQRFKEFQTEVE 451
>ref|XP_006448399.1| hypothetical protein CICLE_v10014433mg [Citrus clementina]
gi|557551010|gb|ESR61639.1| hypothetical protein
CICLE_v10014433mg [Citrus clementina]
Length = 714
Score = 167 bits (423), Expect = 1e-39
Identities = 86/102 (84%), Positives = 92/102 (90%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
+ KD++ Y K KG KECLCFR+DESETLSENVE YDLVPLD+QVAFDLDELLKASAF
Sbjct: 352 EGKDENCYA--KGGKGRKECLCFRKDESETLSENVEQYDLVPLDTQVAFDLDELLKASAF 409
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 410 VLGKSGIGIVYKVVLEDGHTLAVRRLGEGGSQRFKEFQTEVE 451
>ref|XP_004164156.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Cucumis sativus]
Length = 718
Score = 167 bits (423), Expect = 1e-39
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K D+S YGFEK KG K+CLCF++ ESE +SE++E +DLVPLDSQV FDLDELLKASAF
Sbjct: 356 KKADQSSYGFEKGEKGRKDCLCFQKSESENVSEHIEQFDLVPLDSQVTFDLDELLKASAF 415
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G+TLAVRRLGEGGSQR KEFQTEVE
Sbjct: 416 VLGKSGIGIVYKVVLEDGLTLAVRRLGEGGSQRLKEFQTEVE 457
>ref|XP_004135080.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Cucumis sativus]
Length = 718
Score = 167 bits (423), Expect = 1e-39
Identities = 81/102 (79%), Positives = 91/102 (89%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K D+S YGFEK KG K+CLCF++ ESE +SE++E +DLVPLDSQV FDLDELLKASAF
Sbjct: 356 KKADQSSYGFEKGEKGRKDCLCFQKSESENVSEHIEQFDLVPLDSQVTFDLDELLKASAF 415
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGKSGIGIVYKVVLE+G+TLAVRRLGEGGSQR KEFQTEVE
Sbjct: 416 VLGKSGIGIVYKVVLEDGLTLAVRRLGEGGSQRLKEFQTEVE 457
>ref|XP_004161994.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Cucumis sativus]
Length = 717
Score = 165 bits (418), Expect = 5e-39
Identities = 82/102 (80%), Positives = 93/102 (91%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
++KDK G+ +K KG ECLCFR+DESE++S+NVE YDLVPLD+QV FDLDELLKASAF
Sbjct: 349 RTKDKMGHNSDKG-KGRNECLCFRKDESESVSQNVEQYDLVPLDAQVGFDLDELLKASAF 407
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
V+GKSGIGIVYKVVLE+GVTLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 408 VIGKSGIGIVYKVVLEDGVTLAVRRLGEGGSQRFKEFQTEVE 449
>ref|XP_004147142.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Cucumis sativus]
Length = 717
Score = 165 bits (418), Expect = 5e-39
Identities = 82/102 (80%), Positives = 93/102 (91%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
++KDK G+ +K KG ECLCFR+DESE++S+NVE YDLVPLD+QV FDLDELLKASAF
Sbjct: 349 RTKDKMGHNSDKG-KGRNECLCFRKDESESVSQNVEQYDLVPLDAQVGFDLDELLKASAF 407
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
V+GKSGIGIVYKVVLE+GVTLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 408 VIGKSGIGIVYKVVLEDGVTLAVRRLGEGGSQRFKEFQTEVE 449
>ref|XP_002311473.2| leucine-rich repeat transmembrane protein kinase [Populus
trichocarpa] gi|550332911|gb|EEE88840.2| leucine-rich
repeat transmembrane protein kinase [Populus
trichocarpa]
Length = 724
Score = 161 bits (408), Expect = 8e-38
Identities = 83/102 (81%), Positives = 88/102 (86%)
Frame = +2
Query: 8 KSKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAF 187
K KD++ GFEK K K CL FR+DESETLSENVE DLVPLD+QVAFDLDELLKASAF
Sbjct: 359 KDKDENDNGFEKGGKRRKGCLRFRKDESETLSENVEQCDLVPLDAQVAFDLDELLKASAF 418
Query: 188 VLGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
VLGK GIGI YKVVLE+G TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 419 VLGKGGIGIAYKVVLEDGYTLAVRRLGEGGSQRFKEFQTEVE 460
>ref|XP_004497046.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Cicer arietinum]
Length = 711
Score = 161 bits (407), Expect = 1e-37
Identities = 81/101 (80%), Positives = 88/101 (87%)
Frame = +2
Query: 11 SKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAFV 190
++D+ K K KECLCFR+DESE LS+NVE YDLVPLDSQVAFDLDELLKASAFV
Sbjct: 353 NQDQDDNDVNKGRKRRKECLCFRKDESEALSDNVEQYDLVPLDSQVAFDLDELLKASAFV 412
Query: 191 LGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
LGKSGIGI+YKVVLEEG+ LAVRRLGEGGSQRFKEFQTEVE
Sbjct: 413 LGKSGIGIMYKVVLEEGLALAVRRLGEGGSQRFKEFQTEVE 453
>ref|XP_003555049.1| PREDICTED: probable inactive leucine-rich repeat receptor-like
protein kinase At1g66830-like [Glycine max]
Length = 710
Score = 159 bits (402), Expect = 4e-37
Identities = 80/101 (79%), Positives = 90/101 (89%)
Frame = +2
Query: 11 SKDKSGYGFEKASKGSKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAFV 190
++D+ GF+K + KECLCFR+DESETLS++ E YDLVPLD+QVAFDLDELLKASAFV
Sbjct: 352 TQDQEEKGFDKGRRLRKECLCFRKDESETLSDHDEQYDLVPLDAQVAFDLDELLKASAFV 411
Query: 191 LGKSGIGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
LGKS IGIVYKVVLEEG+ LAVRRLGEGGSQRFKEFQTEVE
Sbjct: 412 LGKSEIGIVYKVVLEEGLNLAVRRLGEGGSQRFKEFQTEVE 452
>ref|XP_006290656.1| hypothetical protein CARUB_v10016748mg [Capsella rubella]
gi|482559363|gb|EOA23554.1| hypothetical protein
CARUB_v10016748mg [Capsella rubella]
Length = 720
Score = 157 bits (396), Expect = 2e-36
Identities = 80/96 (83%), Positives = 88/96 (91%), Gaps = 1/96 (1%)
Frame = +2
Query: 29 YGFEKASKG-SKECLCFRRDESETLSENVEHYDLVPLDSQVAFDLDELLKASAFVLGKSG 205
+GFEK SK + ECLCFR+DESETLSENVEH D+V LD+QV F+L+ELLKASAFVLGKSG
Sbjct: 359 FGFEKESKKRASECLCFRKDESETLSENVEHCDIVALDAQVVFNLEELLKASAFVLGKSG 418
Query: 206 IGIVYKVVLEEGVTLAVRRLGEGGSQRFKEFQTEVE 313
IGIVYKVVLE G+TLAVRRLGEGGSQRFKEFQTEVE
Sbjct: 419 IGIVYKVVLENGLTLAVRRLGEGGSQRFKEFQTEVE 454