BLASTX nr result
ID: Mentha22_contig00014770
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00014770
(389 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU36933.1| hypothetical protein MIMGU_mgv1a022265mg, partial... 102 4e-20
ref|XP_007040087.1| Uncharacterized protein isoform 5 [Theobroma... 93 4e-17
ref|XP_007040084.1| Uncharacterized protein isoform 2 [Theobroma... 93 4e-17
ref|XP_007040083.1| Uncharacterized protein isoform 1 [Theobroma... 93 4e-17
ref|XP_006477357.1| PREDICTED: protein FAM63B-like [Citrus sinen... 91 2e-16
ref|XP_006440500.1| hypothetical protein CICLE_v10019075mg [Citr... 90 3e-16
ref|XP_007040085.1| Uncharacterized protein isoform 3 [Theobroma... 89 6e-16
ref|XP_004245663.1| PREDICTED: uncharacterized protein LOC101249... 83 5e-14
ref|XP_004245664.1| PREDICTED: uncharacterized protein LOC101249... 81 1e-13
ref|XP_006358850.1| PREDICTED: protein FAM63B-like [Solanum tube... 81 2e-13
ref|XP_002509683.1| conserved hypothetical protein [Ricinus comm... 81 2e-13
ref|XP_003611174.1| Protein FAM63A [Medicago truncatula] gi|3555... 80 3e-13
ref|XP_002299570.2| hypothetical protein POPTR_0001s08530g [Popu... 79 7e-13
ref|XP_006573752.1| PREDICTED: protein FAM63B-like isoform X2 [G... 77 2e-12
ref|XP_003516684.1| PREDICTED: protein FAM63B-like isoform X1 [G... 77 2e-12
ref|XP_004298726.1| PREDICTED: uncharacterized protein LOC101309... 77 2e-12
ref|XP_007158448.1| hypothetical protein PHAVU_002G153200g [Phas... 77 3e-12
ref|XP_006368742.1| hypothetical protein POPTR_0001s08530g [Popu... 76 4e-12
ref|XP_003538713.1| PREDICTED: protein FAM63B-like [Glycine max] 75 9e-12
emb|CBI34769.3| unnamed protein product [Vitis vinifera] 75 9e-12
>gb|EYU36933.1| hypothetical protein MIMGU_mgv1a022265mg, partial [Mimulus
guttatus]
Length = 644
Score = 102 bits (255), Expect = 4e-20
Identities = 56/96 (58%), Positives = 69/96 (71%), Gaps = 1/96 (1%)
Frame = +1
Query: 103 DGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVDS-EPIYEGEDSILDSGTVANVNREP 279
D SS AD DSS Q+ + D+P+ PSSVD+ EP+YEGE+ I+ G V N EP
Sbjct: 383 DRDTSSLLTTAADLDSSRGQVGKIDKPQTFPSSVDNTEPMYEGEERIMSPGPVVYENPEP 442
Query: 280 MYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+YEGEVVLAEQVD GCV+DGDF+ DRISVKQGE++
Sbjct: 443 VYEGEVVLAEQVDTGCVNDGDFN--DRISVKQGEVI 476
>ref|XP_007040087.1| Uncharacterized protein isoform 5 [Theobroma cacao]
gi|508777332|gb|EOY24588.1| Uncharacterized protein
isoform 5 [Theobroma cacao]
Length = 677
Score = 92.8 bits (229), Expect = 4e-17
Identities = 51/103 (49%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D++N + + SS + ADSDSSS +++ D PE SS+D SEPIYEGE+ ILDS T
Sbjct: 444 DNVNGCDMTEVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTT 503
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+REP+YEGEV+LAEQ D V+ + SKD I+ +QGE++
Sbjct: 504 TYEDREPIYEGEVILAEQADKKAVEGCNVRSKDEITPQQGELI 546
>ref|XP_007040084.1| Uncharacterized protein isoform 2 [Theobroma cacao]
gi|508777329|gb|EOY24585.1| Uncharacterized protein
isoform 2 [Theobroma cacao]
Length = 752
Score = 92.8 bits (229), Expect = 4e-17
Identities = 51/103 (49%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D++N + + SS + ADSDSSS +++ D PE SS+D SEPIYEGE+ ILDS T
Sbjct: 444 DNVNGCDMTEVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTT 503
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+REP+YEGEV+LAEQ D V+ + SKD I+ +QGE++
Sbjct: 504 TYEDREPIYEGEVILAEQADKKAVEGCNVRSKDEITPQQGELI 546
>ref|XP_007040083.1| Uncharacterized protein isoform 1 [Theobroma cacao]
gi|508777328|gb|EOY24584.1| Uncharacterized protein
isoform 1 [Theobroma cacao]
Length = 733
Score = 92.8 bits (229), Expect = 4e-17
Identities = 51/103 (49%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D++N + + SS + ADSDSSS +++ D PE SS+D SEPIYEGE+ ILDS T
Sbjct: 444 DNVNGCDMTEVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTT 503
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+REP+YEGEV+LAEQ D V+ + SKD I+ +QGE++
Sbjct: 504 TYEDREPIYEGEVILAEQADKKAVEGCNVRSKDEITPQQGELI 546
>ref|XP_006477357.1| PREDICTED: protein FAM63B-like [Citrus sinensis]
Length = 709
Score = 90.5 bits (223), Expect = 2e-16
Identities = 50/103 (48%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D+++ + + S ++ DSDSSS ++ +D PE SSVD SEPIYEGE+ ILDSGT
Sbjct: 419 DNLSRCSTTEVSCLSVHNGDSDSSSGRVQLSDVPENFTSSVDGSEPIYEGEECILDSGTA 478
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+REP+YEGEV+LAEQ D +D + SKD I+ +QGE++
Sbjct: 479 MYEDREPVYEGEVILAEQADKSTLDACNVWSKDEITQQQGELI 521
>ref|XP_006440500.1| hypothetical protein CICLE_v10019075mg [Citrus clementina]
gi|557542762|gb|ESR53740.1| hypothetical protein
CICLE_v10019075mg [Citrus clementina]
Length = 709
Score = 90.1 bits (222), Expect = 3e-16
Identities = 50/103 (48%), Positives = 69/103 (66%), Gaps = 1/103 (0%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D++N + + ++ DSDSSS ++ +D PE SSVD SEPIYEGE+ ILDSGT
Sbjct: 419 DNLNRCSTTEVLCLSVHNGDSDSSSGRIQLSDVPENFTSSVDGSEPIYEGEECILDSGTA 478
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
+REP+YEGEV+LAEQ D +D + SKD I+ +QGE++
Sbjct: 479 MYEDREPVYEGEVILAEQADKSTLDACNVWSKDEITQQQGELI 521
>ref|XP_007040085.1| Uncharacterized protein isoform 3 [Theobroma cacao]
gi|590677679|ref|XP_007040086.1| Uncharacterized protein
isoform 3 [Theobroma cacao]
gi|590677687|ref|XP_007040088.1| Uncharacterized protein
isoform 3 [Theobroma cacao] gi|508777330|gb|EOY24586.1|
Uncharacterized protein isoform 3 [Theobroma cacao]
gi|508777331|gb|EOY24587.1| Uncharacterized protein
isoform 3 [Theobroma cacao] gi|508777333|gb|EOY24589.1|
Uncharacterized protein isoform 3 [Theobroma cacao]
Length = 543
Score = 89.0 bits (219), Expect = 6e-16
Identities = 50/100 (50%), Positives = 67/100 (67%), Gaps = 1/100 (1%)
Frame = +1
Query: 82 DSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTV 258
D++N + + SS + ADSDSSS +++ D PE SS+D SEPIYEGE+ ILDS T
Sbjct: 444 DNVNGCDMTEVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTT 503
Query: 259 ANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQG 378
+REP+YEGEV+LAEQ D V+ + SKD I+ +QG
Sbjct: 504 TYEDREPIYEGEVILAEQADKKAVEGCNVRSKDEITPQQG 543
>ref|XP_004245663.1| PREDICTED: uncharacterized protein LOC101249947 isoform 1 [Solanum
lycopersicum]
Length = 694
Score = 82.8 bits (203), Expect = 5e-14
Identities = 47/92 (51%), Positives = 61/92 (66%), Gaps = 1/92 (1%)
Frame = +1
Query: 115 SSRPLNGADSDSSSVQMYRNDEPEPNPSSVDS-EPIYEGEDSILDSGTVANVNREPMYEG 291
SS + A +DSS + + DE E SS+D EP+YEGED IL+S T + +REPMYEG
Sbjct: 412 SSLSIPSAATDSSDGRGHDTDEAETFNSSIDGDEPMYEGEDCILESATTSYQSREPMYEG 471
Query: 292 EVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
EVVLAEQV+ G D +KD I+ K+GE+V
Sbjct: 472 EVVLAEQVNGGSRDVPAIIAKDEITQKEGELV 503
>ref|XP_004245664.1| PREDICTED: uncharacterized protein LOC101249947 isoform 2 [Solanum
lycopersicum]
Length = 656
Score = 81.3 bits (199), Expect = 1e-13
Identities = 47/88 (53%), Positives = 61/88 (69%), Gaps = 1/88 (1%)
Frame = +1
Query: 127 LNGADSDSSSVQMYRNDEPEPNPSSVDS-EPIYEGEDSILDSGTVANVNREPMYEGEVVL 303
L+GA +DSS + + DE E SS+D EP+YEGED IL+S T + +REPMYEGEVVL
Sbjct: 379 LSGA-TDSSDGRGHDTDEAETFNSSIDGDEPMYEGEDCILESATTSYQSREPMYEGEVVL 437
Query: 304 AEQVDAGCVDDGDFSSKDRISVKQGEIV 387
AEQV+ G D +KD I+ K+GE+V
Sbjct: 438 AEQVNGGSRDVPAIIAKDEITQKEGELV 465
>ref|XP_006358850.1| PREDICTED: protein FAM63B-like [Solanum tuberosum]
Length = 694
Score = 80.9 bits (198), Expect = 2e-13
Identities = 45/92 (48%), Positives = 59/92 (64%), Gaps = 1/92 (1%)
Frame = +1
Query: 115 SSRPLNGADSDSSSVQMYRNDEPEPNPSSVDS-EPIYEGEDSILDSGTVANVNREPMYEG 291
SS + A DSS + + DE E SS+D EP+YEGED IL+S T + +REPMYEG
Sbjct: 412 SSSSIPSAAPDSSGGRQHDTDEAETFNSSIDGDEPMYEGEDCILESATTSYQSREPMYEG 471
Query: 292 EVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
EV+LAEQV+ D + KD I+ K+GE+V
Sbjct: 472 EVILAEQVNGDSRDVPEIIVKDEITQKEGELV 503
>ref|XP_002509683.1| conserved hypothetical protein [Ricinus communis]
gi|223549582|gb|EEF51070.1| conserved hypothetical
protein [Ricinus communis]
Length = 730
Score = 80.9 bits (198), Expect = 2e-13
Identities = 51/114 (44%), Positives = 66/114 (57%), Gaps = 2/114 (1%)
Frame = +1
Query: 52 LDQVTTTSNRDSINSHNDGKDSSR-PLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYE 225
L+ + I+S D ++S + DSDSSS ++ D E SSVD SEPIYE
Sbjct: 438 LESIANVHEAADISSGRDPAEASGLSMPNPDSDSSSGRLQNVDVTETFTSSVDGSEPIYE 497
Query: 226 GEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
GE+ ILDSGT +REPMYEGEV+LAEQ D KD I+ +QGE++
Sbjct: 498 GEECILDSGTATFEDREPMYEGEVILAEQADK------SVRLKDEITPQQGELI 545
>ref|XP_003611174.1| Protein FAM63A [Medicago truncatula] gi|355512509|gb|AES94132.1|
Protein FAM63A [Medicago truncatula]
Length = 737
Score = 80.1 bits (196), Expect = 3e-13
Identities = 42/84 (50%), Positives = 58/84 (69%), Gaps = 1/84 (1%)
Frame = +1
Query: 139 DSDSSSVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQV 315
DSDSS ++ ++ D E PS+VD SEP+YEGE+ +LD+ T NREP+YEGE VL EQ
Sbjct: 467 DSDSSVIRFHQTDASEAFPSTVDGSEPMYEGEECVLDTKTGNFENREPVYEGEAVLQEQA 526
Query: 316 DAGCVDDGDFSSKDRISVKQGEIV 387
D +D D +K+ I+ +QGE+V
Sbjct: 527 DKSTLDALDPRAKEEITPEQGELV 550
>ref|XP_002299570.2| hypothetical protein POPTR_0001s08530g [Populus trichocarpa]
gi|550346841|gb|EEE84375.2| hypothetical protein
POPTR_0001s08530g [Populus trichocarpa]
Length = 686
Score = 79.0 bits (193), Expect = 7e-13
Identities = 51/114 (44%), Positives = 68/114 (59%), Gaps = 1/114 (0%)
Frame = +1
Query: 49 VLDQVTTTSNRDSINSHNDGKDSSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYE 225
+ D V+ +S+ + D S+ P+ +S+SS +M D P+ SSVD SEPIYE
Sbjct: 385 IQDAVSLSSDISNGRDKEDVPGSASPV--LESNSSIGRMQNVDAPDTFTSSVDGSEPIYE 442
Query: 226 GEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
GE+ ILD+GT REPMYEGEVVLAEQ D SKD I+ +QGE++
Sbjct: 443 GEECILDTGTSNYEEREPMYEGEVVLAEQADR------TVRSKDEITPQQGELI 490
>ref|XP_006573752.1| PREDICTED: protein FAM63B-like isoform X2 [Glycine max]
Length = 707
Score = 77.4 bits (189), Expect = 2e-12
Identities = 50/119 (42%), Positives = 70/119 (58%), Gaps = 6/119 (5%)
Frame = +1
Query: 49 VLDQVTTTSNRDSINSHNDGKDS-----SRPLNGADSDSSSVQMYRNDEPEPNPSSVD-S 210
V DQ T T+ + + G D+ S P +SDSSSV+ ++ D SSV S
Sbjct: 417 VHDQSTPTTIDHEVVGESHGPDATGLSFSSP-GHTNSDSSSVRYHQTDVSGALTSSVQGS 475
Query: 211 EPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
EPIYEGE+ +LD+ T +REP+YEGEVVLAEQ D + D +KD ++ +QGE++
Sbjct: 476 EPIYEGEECVLDTRTGNFEDREPVYEGEVVLAEQADRSTLVAPDLRAKDELTPEQGELI 534
>ref|XP_003516684.1| PREDICTED: protein FAM63B-like isoform X1 [Glycine max]
Length = 726
Score = 77.4 bits (189), Expect = 2e-12
Identities = 50/119 (42%), Positives = 70/119 (58%), Gaps = 6/119 (5%)
Frame = +1
Query: 49 VLDQVTTTSNRDSINSHNDGKDS-----SRPLNGADSDSSSVQMYRNDEPEPNPSSVD-S 210
V DQ T T+ + + G D+ S P +SDSSSV+ ++ D SSV S
Sbjct: 417 VHDQSTPTTIDHEVVGESHGPDATGLSFSSP-GHTNSDSSSVRYHQTDVSGALTSSVQGS 475
Query: 211 EPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
EPIYEGE+ +LD+ T +REP+YEGEVVLAEQ D + D +KD ++ +QGE++
Sbjct: 476 EPIYEGEECVLDTRTGNFEDREPVYEGEVVLAEQADRSTLVAPDLRAKDELTPEQGELI 534
>ref|XP_004298726.1| PREDICTED: uncharacterized protein LOC101309929 [Fragaria vesca
subsp. vesca]
Length = 718
Score = 77.0 bits (188), Expect = 2e-12
Identities = 56/139 (40%), Positives = 73/139 (52%), Gaps = 16/139 (11%)
Frame = +1
Query: 19 PESNE--IQHNH---------VLDQVTTTSNRDSINSHNDGKDSSR----PLNGADSDSS 153
P+SN+ I NH + D V +N D I N S+ P + SDSS
Sbjct: 392 PDSNDESIDENHTDVLKGGEKIEDHVNLATNADGIVEINKQNGSNMTEVIPASNVGSDSS 451
Query: 154 SVQMYRNDEPEPNPSSVD-SEPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCV 330
S +M E E SS + SEPIY GE+ ILDSG+ EP+YEGE VLA+Q D G +
Sbjct: 452 SGRMLGIGESEALMSSGEGSEPIYAGEECILDSGSTVFEEGEPVYEGEAVLAKQADKGML 511
Query: 331 DDGDFSSKDRISVKQGEIV 387
D SKD I+ +QGE++
Sbjct: 512 ---DARSKDEITPQQGELI 527
>ref|XP_007158448.1| hypothetical protein PHAVU_002G153200g [Phaseolus vulgaris]
gi|561031863|gb|ESW30442.1| hypothetical protein
PHAVU_002G153200g [Phaseolus vulgaris]
Length = 720
Score = 76.6 bits (187), Expect = 3e-12
Identities = 48/118 (40%), Positives = 68/118 (57%), Gaps = 5/118 (4%)
Frame = +1
Query: 49 VLDQVTTTSNRDSINSHNDGKDSSRPLN----GADSDSSSVQMYRNDEPEPNPSSVD-SE 213
V DQ T + + + G D+++ L ADSDS S++ + D SSV SE
Sbjct: 415 VHDQSTPITIDHEVVGESHGPDATKSLQLSPGHADSDSCSIRYQQTDVSGALTSSVHGSE 474
Query: 214 PIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
PIYEGE+ +LD T + REP+YEGEVVLAEQ D + D +KD ++ +QGE++
Sbjct: 475 PIYEGEECVLDRRTFED--REPVYEGEVVLAEQADKSTLASPDLRAKDELTPEQGELI 530
>ref|XP_006368742.1| hypothetical protein POPTR_0001s08530g [Populus trichocarpa]
gi|550346842|gb|ERP65311.1| hypothetical protein
POPTR_0001s08530g [Populus trichocarpa]
Length = 721
Score = 76.3 bits (186), Expect = 4e-12
Identities = 51/113 (45%), Positives = 67/113 (59%), Gaps = 4/113 (3%)
Frame = +1
Query: 61 VTTTSNRDSINSHNDGKD---SSRPLNGADSDSSSVQMYRNDEPEPNPSSVD-SEPIYEG 228
+T I++ D +D S+ P+ +S+SS +M D P+ SSVD SEPIYEG
Sbjct: 421 ITEVHEPADISNGRDKEDVPGSASPV--LESNSSIGRMQNVDAPDTFTSSVDGSEPIYEG 478
Query: 229 EDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
E+ ILD+GT REPMYEGEVVLAEQ D SKD I+ +QGE++
Sbjct: 479 EECILDTGTSNYEEREPMYEGEVVLAEQADR------TVRSKDEITPQQGELI 525
>ref|XP_003538713.1| PREDICTED: protein FAM63B-like [Glycine max]
Length = 723
Score = 75.1 bits (183), Expect = 9e-12
Identities = 49/119 (41%), Positives = 69/119 (57%), Gaps = 6/119 (5%)
Frame = +1
Query: 49 VLDQVTTTSNRDSINSHNDGKDS-----SRPLNGADSDSSSVQMYRNDEPEPNPSSVD-S 210
V DQ T T+ + + G D+ S P +SDSSSV+ ++ D S V S
Sbjct: 414 VHDQSTPTTIDHEVVDESHGPDATVLSFSSP-GHTNSDSSSVRYHQTDVSGALTSCVQGS 472
Query: 211 EPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVKQGEIV 387
EPIYEGE+ +LD+ T +REP+YEGEVVLAEQ D + D +KD ++ +QGE++
Sbjct: 473 EPIYEGEECVLDTRTGNFEDREPVYEGEVVLAEQSDKSTLAAPDLRAKDELTPEQGELI 531
>emb|CBI34769.3| unnamed protein product [Vitis vinifera]
Length = 662
Score = 75.1 bits (183), Expect = 9e-12
Identities = 38/65 (58%), Positives = 44/65 (67%)
Frame = +1
Query: 193 PSSVDSEPIYEGEDSILDSGTVANVNREPMYEGEVVLAEQVDAGCVDDGDFSSKDRISVK 372
PS D EPIYEGE+ ILDS T +REPMYEGEVVLAEQ D VD + KD ++
Sbjct: 412 PSVDDGEPIYEGEECILDSNTTIYEDREPMYEGEVVLAEQADKDSVDSCNIGFKDELTPL 471
Query: 373 QGEIV 387
QGE+V
Sbjct: 472 QGELV 476