BLASTX nr result
ID: Mentha22_contig00005102
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00005102
(504 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU19755.1| hypothetical protein MIMGU_mgv1a000969mg [Mimulus... 88 1e-20
ref|XP_006347609.1| PREDICTED: alanine--tRNA ligase-like [Solanu... 86 2e-17
gb|EPS72880.1| hypothetical protein M569_01869, partial [Genlise... 86 2e-17
ref|XP_004235270.1| PREDICTED: alanine--tRNA ligase-like [Solanu... 84 2e-16
ref|NP_175439.2| Alanyl-tRNA synthetase [Arabidopsis thaliana] g... 80 8e-16
ref|NP_001185186.1| Alanyl-tRNA synthetase [Arabidopsis thaliana... 80 8e-16
gb|AAD50044.1|AC007980_9 cytosolic tRNA-Ala synthetase [Arabidop... 80 8e-16
ref|XP_006305941.1| hypothetical protein CARUB_v10011180mg [Caps... 80 1e-15
ref|XP_002894237.1| cytosolic tRNA-Ala synthetase [Arabidopsis l... 80 1e-15
emb|CAA80380.1| mitochondrial tRNA-Ala synthetase [Arabidopsis t... 78 2e-15
pir||S32671 alanine-tRNA ligase (EC 6.1.1.7) - Arabidopsis thali... 78 2e-15
emb|CAA80381.1| cytosolic tRNA-Ala synthetase [Arabidopsis thali... 78 2e-15
emb|CBI27126.3| unnamed protein product [Vitis vinifera] 77 4e-15
ref|XP_002277341.1| PREDICTED: alanyl-tRNA synthetase-like [Viti... 77 4e-15
emb|CAA73145.1| alanyl t-RNA synthetase [Arabidopsis thaliana] 80 4e-15
ref|XP_003537781.2| PREDICTED: alanine--tRNA ligase-like isoform... 77 2e-14
ref|XP_006590811.1| PREDICTED: alanine--tRNA ligase-like isoform... 77 2e-14
ref|XP_006393173.1| hypothetical protein EUTSA_v10011205mg [Eutr... 76 2e-14
ref|XP_006393172.1| hypothetical protein EUTSA_v10011205mg [Eutr... 76 2e-14
ref|XP_006393171.1| hypothetical protein EUTSA_v10011205mg [Eutr... 76 2e-14
>gb|EYU19755.1| hypothetical protein MIMGU_mgv1a000969mg [Mimulus guttatus]
Length = 927
Score = 88.2 bits (217), Expect(2) = 1e-20
Identities = 43/56 (76%), Positives = 49/56 (87%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAE 335
GKTFCI VDVG+DTTAIREAVVKVME+KGMAVMV + DE +NKAFVCAGVP + +
Sbjct: 817 GKTFCISLVDVGADTTAIREAVVKVMEKKGMAVMVFSIDEKVNKAFVCAGVPEDGK 872
Score = 37.0 bits (84), Expect(2) = 1e-20
Identities = 17/22 (77%), Positives = 20/22 (90%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKLT 240
SD+SR+E A+DVAESFA MKLT
Sbjct: 906 SDSSRVEAAMDVAESFAAMKLT 927
>ref|XP_006347609.1| PREDICTED: alanine--tRNA ligase-like [Solanum tuberosum]
Length = 1009
Score = 86.3 bits (212), Expect(2) = 2e-17
Identities = 44/74 (59%), Positives = 55/74 (74%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEEKGV 323
GK +CIL++ VG DT A+REAVVKVMEQKGMAV+V ++DEA NK VCAGVP E +K
Sbjct: 898 GKAYCILQIGVGLDTAAVREAVVKVMEQKGMAVLVFSKDEAANKVLVCAGVP-EKGDKCQ 956
Query: 322 LLKARGVMHRALKP 281
L + ++ ALKP
Sbjct: 957 QLNVKDWLNAALKP 970
Score = 28.1 bits (61), Expect(2) = 2e-17
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+D S+++ A+DVA SFA MKL
Sbjct: 988 TDISKVDEAMDVAASFAAMKL 1008
>gb|EPS72880.1| hypothetical protein M569_01869, partial [Genlisea aurea]
Length = 950
Score = 85.5 bits (210), Expect(2) = 2e-17
Identities = 40/57 (70%), Positives = 47/57 (82%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GK FCI V+VGSDTTAIREAV+KVM+ KGMAVMV ++DE NKAFVCAG+P + E
Sbjct: 844 GKNFCISVVEVGSDTTAIREAVIKVMDDKGMAVMVFSKDEEANKAFVCAGIPEQLSE 900
Score = 28.9 bits (63), Expect(2) = 2e-17
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
S AS IE+A+DVAE FA +KL
Sbjct: 930 SYASGIESAVDVAEKFAALKL 950
>ref|XP_004235270.1| PREDICTED: alanine--tRNA ligase-like [Solanum lycopersicum]
Length = 1010
Score = 84.0 bits (206), Expect(2) = 2e-16
Identities = 43/74 (58%), Positives = 54/74 (72%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEEKGV 323
GK +CIL++ VG DT A+REAVVKVMEQKGMAV+V ++DEA K VCAGVP E +K
Sbjct: 899 GKAYCILQIGVGLDTAAVREAVVKVMEQKGMAVLVFSKDEAAKKVLVCAGVP-EKGDKCK 957
Query: 322 LLKARGVMHRALKP 281
L + ++ ALKP
Sbjct: 958 QLNVKDWLNAALKP 971
Score = 26.9 bits (58), Expect(2) = 2e-16
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+D S+++ A+DVA SFA +KL
Sbjct: 989 TDISKVDEAMDVAASFAALKL 1009
>ref|NP_175439.2| Alanyl-tRNA synthetase [Arabidopsis thaliana]
gi|21542451|sp|P36428.3|SYA_ARATH RecName:
Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA
synthetase; Short=AlaRS; Flags: Precursor
gi|332194403|gb|AEE32524.1| Alanyl-tRNA synthetase
[Arabidopsis thaliana]
Length = 1003
Score = 79.7 bits (195), Expect(2) = 8e-16
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 892 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 948
Score = 29.3 bits (64), Expect(2) = 8e-16
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 982 TDASQVQAALDMASSFASMKL 1002
>ref|NP_001185186.1| Alanyl-tRNA synthetase [Arabidopsis thaliana]
gi|332194404|gb|AEE32525.1| Alanyl-tRNA synthetase
[Arabidopsis thaliana]
Length = 963
Score = 79.7 bits (195), Expect(2) = 8e-16
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 852 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 908
Score = 29.3 bits (64), Expect(2) = 8e-16
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 942 TDASQVQAALDMASSFASMKL 962
>gb|AAD50044.1|AC007980_9 cytosolic tRNA-Ala synthetase [Arabidopsis thaliana]
gi|27311841|gb|AAO00886.1| Unknown protein [Arabidopsis
thaliana] gi|31711780|gb|AAP68246.1| At1g50200
[Arabidopsis thaliana]
Length = 955
Score = 79.7 bits (195), Expect(2) = 8e-16
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 844 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 900
Score = 29.3 bits (64), Expect(2) = 8e-16
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 934 TDASQVQAALDMASSFASMKL 954
>ref|XP_006305941.1| hypothetical protein CARUB_v10011180mg [Capsella rubella]
gi|482574652|gb|EOA38839.1| hypothetical protein
CARUB_v10011180mg [Capsella rubella]
Length = 1003
Score = 79.7 bits (195), Expect(2) = 1e-15
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 892 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 948
Score = 28.9 bits (63), Expect(2) = 1e-15
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 982 TDASQVQAALDMAASFASMKL 1002
>ref|XP_002894237.1| cytosolic tRNA-Ala synthetase [Arabidopsis lyrata subsp. lyrata]
gi|297340079|gb|EFH70496.1| cytosolic tRNA-Ala synthetase
[Arabidopsis lyrata subsp. lyrata]
Length = 955
Score = 79.7 bits (195), Expect(2) = 1e-15
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 844 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 900
Score = 28.9 bits (63), Expect(2) = 1e-15
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 934 TDASQVQAALDMAASFASMKL 954
>emb|CAA80380.1| mitochondrial tRNA-Ala synthetase [Arabidopsis thaliana]
Length = 1003
Score = 78.2 bits (191), Expect(2) = 2e-15
Identities = 34/57 (59%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A++EAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 892 GKTFCIIQLDVGLDAAAVQEAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 948
Score = 29.3 bits (64), Expect(2) = 2e-15
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 982 TDASQVQAALDMASSFASMKL 1002
>pir||S32671 alanine-tRNA ligase (EC 6.1.1.7) - Arabidopsis thaliana (fragment)
Length = 989
Score = 78.2 bits (191), Expect(2) = 2e-15
Identities = 34/57 (59%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A++EAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 878 GKTFCIIQLDVGLDAAAVQEAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 934
Score = 29.3 bits (64), Expect(2) = 2e-15
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 968 TDASQVQAALDMASSFASMKL 988
>emb|CAA80381.1| cytosolic tRNA-Ala synthetase [Arabidopsis thaliana]
Length = 955
Score = 78.2 bits (191), Expect(2) = 2e-15
Identities = 34/57 (59%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A++EAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 844 GKTFCIIQLDVGLDAAAVQEAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 900
Score = 29.3 bits (64), Expect(2) = 2e-15
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS+++ A+D+A SFA MKL
Sbjct: 934 TDASQVQAALDMASSFASMKL 954
>emb|CBI27126.3| unnamed protein product [Vitis vinifera]
Length = 1026
Score = 77.0 bits (188), Expect(2) = 4e-15
Identities = 36/57 (63%), Positives = 46/57 (80%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI VDVG DTTA+REAV+KV+EQKG++VM+ + DE NKA V AGVP + ++
Sbjct: 915 GKTFCISHVDVGLDTTAVREAVLKVIEQKGISVMIFSTDETTNKAVVYAGVPQKGDK 971
Score = 29.6 bits (65), Expect(2) = 4e-15
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS ++ A+DVAE+FA MKL
Sbjct: 1005 TDASHVKEAMDVAENFASMKL 1025
>ref|XP_002277341.1| PREDICTED: alanyl-tRNA synthetase-like [Vitis vinifera]
Length = 1002
Score = 77.0 bits (188), Expect(2) = 4e-15
Identities = 36/57 (63%), Positives = 46/57 (80%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI VDVG DTTA+REAV+KV+EQKG++VM+ + DE NKA V AGVP + ++
Sbjct: 891 GKTFCISHVDVGLDTTAVREAVLKVIEQKGISVMIFSTDETTNKAVVYAGVPQKGDK 947
Score = 29.6 bits (65), Expect(2) = 4e-15
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS ++ A+DVAE+FA MKL
Sbjct: 981 TDASHVKEAMDVAENFASMKL 1001
>emb|CAA73145.1| alanyl t-RNA synthetase [Arabidopsis thaliana]
Length = 251
Score = 79.7 bits (195), Expect(2) = 4e-15
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GKTFCI+++DVG D A+REAV KVME+KGM++MV + DE+ NKA VCAGVP ++++
Sbjct: 140 GKTFCIIQLDVGLDAAAVREAVSKVMEKKGMSIMVFSTDESTNKAVVCAGVPEKSDQ 196
Score = 26.9 bits (58), Expect(2) = 4e-15
Identities = 11/21 (52%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DA +++ A+D+A SFA MKL
Sbjct: 230 TDAFQVQAALDMASSFASMKL 250
>ref|XP_003537781.2| PREDICTED: alanine--tRNA ligase-like isoform X1 [Glycine max]
Length = 999
Score = 77.0 bits (188), Expect(2) = 2e-14
Identities = 39/59 (66%), Positives = 45/59 (76%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEEKG 326
GKTFCI VDVG D A+REAV KVM+QKG++VMV + DE+ NKA VCAGVP EKG
Sbjct: 889 GKTFCISLVDVGLDVAAVREAVTKVMDQKGLSVMVFSTDESTNKAVVCAGVP----EKG 943
Score = 27.7 bits (60), Expect(2) = 2e-14
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKLT 240
++AS + AID+AE FA MKL+
Sbjct: 978 TEASHVNEAIDLAEKFASMKLS 999
>ref|XP_006590811.1| PREDICTED: alanine--tRNA ligase-like isoform X2 [Glycine max]
Length = 824
Score = 77.0 bits (188), Expect(2) = 2e-14
Identities = 39/59 (66%), Positives = 45/59 (76%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEEKG 326
GKTFCI VDVG D A+REAV KVM+QKG++VMV + DE+ NKA VCAGVP EKG
Sbjct: 714 GKTFCISLVDVGLDVAAVREAVTKVMDQKGLSVMVFSTDESTNKAVVCAGVP----EKG 768
Score = 27.7 bits (60), Expect(2) = 2e-14
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKLT 240
++AS + AID+AE FA MKL+
Sbjct: 803 TEASHVNEAIDLAEKFASMKLS 824
>ref|XP_006393173.1| hypothetical protein EUTSA_v10011205mg [Eutrema salsugineum]
gi|557089751|gb|ESQ30459.1| hypothetical protein
EUTSA_v10011205mg [Eutrema salsugineum]
Length = 1005
Score = 76.3 bits (186), Expect(2) = 2e-14
Identities = 34/57 (59%), Positives = 47/57 (82%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GK FCI+++DVG D A+REAV KVM++KGM+VMV + DE+ NKA VCAGVP ++++
Sbjct: 894 GKMFCIVQLDVGLDAAAVREAVSKVMQKKGMSVMVFSTDESTNKAVVCAGVPEKSDQ 950
Score = 28.1 bits (61), Expect(2) = 2e-14
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS++ A+D+A SFA MKL
Sbjct: 984 TDASQVNAALDMAASFASMKL 1004
>ref|XP_006393172.1| hypothetical protein EUTSA_v10011205mg [Eutrema salsugineum]
gi|557089750|gb|ESQ30458.1| hypothetical protein
EUTSA_v10011205mg [Eutrema salsugineum]
Length = 1001
Score = 76.3 bits (186), Expect(2) = 2e-14
Identities = 34/57 (59%), Positives = 47/57 (82%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GK FCI+++DVG D A+REAV KVM++KGM+VMV + DE+ NKA VCAGVP ++++
Sbjct: 890 GKMFCIVQLDVGLDAAAVREAVSKVMQKKGMSVMVFSTDESTNKAVVCAGVPEKSDQ 946
Score = 28.1 bits (61), Expect(2) = 2e-14
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS++ A+D+A SFA MKL
Sbjct: 980 TDASQVNAALDMAASFASMKL 1000
>ref|XP_006393171.1| hypothetical protein EUTSA_v10011205mg [Eutrema salsugineum]
gi|557089749|gb|ESQ30457.1| hypothetical protein
EUTSA_v10011205mg [Eutrema salsugineum]
Length = 994
Score = 76.3 bits (186), Expect(2) = 2e-14
Identities = 34/57 (59%), Positives = 47/57 (82%)
Frame = -3
Query: 502 GKTFCILRVDVGSDTTAIREAVVKVMEQKGMAVMVLTRDEALNKAFVCAGVPXEAEE 332
GK FCI+++DVG D A+REAV KVM++KGM+VMV + DE+ NKA VCAGVP ++++
Sbjct: 883 GKMFCIVQLDVGLDAAAVREAVSKVMQKKGMSVMVFSTDESTNKAVVCAGVPEKSDQ 939
Score = 28.1 bits (61), Expect(2) = 2e-14
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -2
Query: 305 SDASRIETAIDVAESFALMKL 243
+DAS++ A+D+A SFA MKL
Sbjct: 973 TDASQVNAALDMAASFASMKL 993