BLASTX nr result
ID: Magnolia22_contig00030332
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Magnolia22_contig00030332
(404 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ACJ76784.1 berberine bridge enzyme 2 [Argemone mexicana] 117 3e-28
ACJ76783.1 berberine bridge enzyme 1 [Argemone mexicana] 117 4e-28
ACO90218.1 berberine bridge enzyme, partial [Eschscholzia califo... 109 4e-27
4PZF_A Chain A, Berberine Bridge Enzyme G164a Variant, A Reticul... 108 4e-25
3D2D_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ... 108 4e-25
4EC3_A Chain A, Structure Of Berberine Bridge Enzyme, H174a Vari... 108 5e-25
3GSY_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ... 108 5e-25
P30986.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberi... 108 5e-25
XP_006846477.1 PREDICTED: reticuline oxidase [Amborella trichopo... 106 2e-24
3FW9_A Chain A, Structure Of Berberine Bridge Enzyme In Complex ... 106 3e-24
3FW8_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant 106 3e-24
3FWA_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Vari... 106 3e-24
BAM44344.1 berberine bridge enzyme [Coptis japonica] 105 5e-24
XP_011626556.1 PREDICTED: reticuline oxidase [Amborella trichopoda] 105 8e-24
ERN14600.1 hypothetical protein AMTR_s00038p00162870 [Amborella ... 105 1e-23
3FW7_A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant 104 1e-23
ACO90226.1 putative berberine bridge enzyme, partial [Papaver br... 100 2e-23
AAU20769.1 berberine bridge enzyme [Thalictrum flavum subsp. gla... 101 2e-22
KCW70998.1 hypothetical protein EUGRSUZ_F04104 [Eucalyptus grandis] 101 2e-22
XP_010063741.1 PREDICTED: reticuline oxidase [Eucalyptus grandis] 101 2e-22
>ACJ76784.1 berberine bridge enzyme 2 [Argemone mexicana]
Length = 536
Score = 117 bits (293), Expect = 3e-28
Identities = 57/106 (53%), Positives = 75/106 (70%), Gaps = 3/106 (2%)
Frame = -3
Query: 309 CACGEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQ 139
CA +D+LSCL+S GV N TT S S Y +L + S +N F +S +SKP I+ P N+
Sbjct: 28 CALSDDILSCLTSNGVHNYTTPSSDSNSDYLRLFHLSIQNPLFKKSTISKPSLIVLPGNK 87
Query: 138 SQVSTAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
++S +RC GSWTIR+R GGHSYEGLS TA+TPFV+ID+MNL+
Sbjct: 88 EELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLN 133
>ACJ76783.1 berberine bridge enzyme 1 [Argemone mexicana]
Length = 554
Score = 117 bits (293), Expect = 4e-28
Identities = 57/106 (53%), Positives = 75/106 (70%), Gaps = 3/106 (2%)
Frame = -3
Query: 309 CACGEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQ 139
CA +D+LSCL+S GV N TT S S Y +L + S +N F +S +SKP I+ P N+
Sbjct: 33 CALSDDILSCLTSNGVHNYTTPSSDSNSDYLRLYHLSIQNPLFEKSTISKPSLIVLPRNK 92
Query: 138 SQVSTAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
++S +RC GSWTIR+R GGHSYEGLS TA+TPFV+ID+MNL+
Sbjct: 93 EELSNTVRCCTRGSWTIRLRSGGHSYEGLSYTADTPFVLIDLMNLN 138
>ACO90218.1 berberine bridge enzyme, partial [Eschscholzia californica]
Length = 242
Score = 109 bits (273), Expect = 4e-27
Identities = 53/103 (51%), Positives = 74/103 (71%), Gaps = 3/103 (2%)
Frame = -3
Query: 300 GEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
G DLLSCL+ GV N T + + S + +LL+ S +N F S +SKP II P ++ ++
Sbjct: 24 GNDLLSCLTFNGVRNHTVFSADSDSDFNRLLHLSIQNPLFQNSLISKPSAIILPGSKEEL 83
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
S IRC R+GSWTIR+R GGHSYEGLS T++TPF+++D+MNL+
Sbjct: 84 SNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILVDLMNLN 126
>4PZF_A Chain A, Berberine Bridge Enzyme G164a Variant, A Reticuline
Dehydrogenase 4PZF_B Chain B, Berberine Bridge Enzyme
G164a Variant, A Reticuline Dehydrogenase 4PZF_C Chain
C, Berberine Bridge Enzyme G164a Variant, A Reticuline
Dehydrogenase 4PZF_D Chain D, Berberine Bridge Enzyme
G164a Variant, A Reticuline Dehydrogenase
Length = 538
Score = 108 bits (271), Expect = 4e-25
Identities = 55/107 (51%), Positives = 75/107 (70%), Gaps = 4/107 (3%)
Frame = -3
Query: 309 CA-CGEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTN 142
CA G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P +
Sbjct: 20 CAEAGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGS 79
Query: 141 QSQVSTAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
+ ++S IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 80 KEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 126
>3D2D_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Reticuline 3D2H_A Chain A, Structure Of Berberine
Bridge Enzyme From Eschscholzia Californica, Monoclinic
Crystal Form 3D2J_A Chain A, Structure Of Berberine
Bridge Enzyme From Eschscholzia Californica, Tetragonal
Crystal Form
Length = 538
Score = 108 bits (271), Expect = 4e-25
Identities = 55/107 (51%), Positives = 75/107 (70%), Gaps = 4/107 (3%)
Frame = -3
Query: 309 CA-CGEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTN 142
CA G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P +
Sbjct: 20 CAEAGNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGS 79
Query: 141 QSQVSTAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
+ ++S IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 80 KEELSNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 126
>4EC3_A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In
Complex With (S)-Reticuline
Length = 519
Score = 108 bits (270), Expect = 5e-25
Identities = 53/103 (51%), Positives = 73/103 (70%), Gaps = 3/103 (2%)
Frame = -3
Query: 300 GEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++
Sbjct: 5 GNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEEL 64
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
S IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 65 SNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 107
>3GSY_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
Dehydroscoulerine
Length = 519
Score = 108 bits (270), Expect = 5e-25
Identities = 53/103 (51%), Positives = 73/103 (70%), Gaps = 3/103 (2%)
Frame = -3
Query: 300 GEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++
Sbjct: 5 GNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEEL 64
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
S IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 65 SNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 107
>P30986.1 RecName: Full=Reticuline oxidase; AltName: Full=Berberine
bridge-forming enzyme; Short=BBE; AltName:
Full=Tetrahydroprotoberberine synthase; Flags: Precursor
AAB20352.1 (S)-reticuline:oxygen oxidoreductase
(methylene-bridge-forming) [Eschscholzia californica]
AAC39358.1 berberine bridge enzyme [Eschscholzia
californica]
Length = 538
Score = 108 bits (270), Expect = 5e-25
Identities = 53/103 (51%), Positives = 73/103 (70%), Gaps = 3/103 (2%)
Frame = -3
Query: 300 GEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++
Sbjct: 24 GNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEEL 83
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
S IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 84 SNTIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 126
>XP_006846477.1 PREDICTED: reticuline oxidase [Amborella trichopoda] ERN08152.1
hypothetical protein AMTR_s00018p00124850 [Amborella
trichopoda]
Length = 526
Score = 106 bits (265), Expect = 2e-24
Identities = 54/105 (51%), Positives = 70/105 (66%), Gaps = 3/105 (2%)
Frame = -3
Query: 306 ACGEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQS 136
A GEDL SCL S GV N T+ S Y L+ S +NLRFT+ + KP +I P +
Sbjct: 19 ALGEDLQSCLLSNGVLNFTSRSQSSDAAYNHFLSFSIQNLRFTKPTMEKPVMVILPETHA 78
Query: 135 QVSTAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
Q+S+ I C++E S IR+RCGGHSYEGLSST + F+IID+MNL+
Sbjct: 79 QLSSTILCSKETSHEIRIRCGGHSYEGLSSTGDRSFIIIDLMNLN 123
>3FW9_A Chain A, Structure Of Berberine Bridge Enzyme In Complex With
(S)-Scoulerine
Length = 495
Score = 106 bits (264), Expect = 3e-24
Identities = 52/101 (51%), Positives = 72/101 (71%), Gaps = 3/101 (2%)
Frame = -3
Query: 294 DLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVST 124
DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++S
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 123 AIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 101
>3FW8_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant
Length = 495
Score = 106 bits (264), Expect = 3e-24
Identities = 52/101 (51%), Positives = 72/101 (71%), Gaps = 3/101 (2%)
Frame = -3
Query: 294 DLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVST 124
DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++S
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 123 AIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 101
>3FWA_A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In
Complex With (S)-Reticuline
Length = 497
Score = 106 bits (264), Expect = 3e-24
Identities = 52/101 (51%), Positives = 72/101 (71%), Gaps = 3/101 (2%)
Frame = -3
Query: 294 DLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVST 124
DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++S
Sbjct: 1 DLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEELSN 60
Query: 123 AIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
IRC R+GSWTIR+R GGHSYEGLS T++TPF++ID+MNL+
Sbjct: 61 TIRCIRKGSWTIRLRSGGHSYEGLSYTSDTPFILIDLMNLN 101
>BAM44344.1 berberine bridge enzyme [Coptis japonica]
Length = 533
Score = 105 bits (263), Expect = 5e-24
Identities = 55/101 (54%), Positives = 68/101 (67%), Gaps = 2/101 (1%)
Frame = -3
Query: 297 EDLLSCLSSGGVANVTT--YCSPSYPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVST 124
+DL SCL GV+N TT + Y +LL S +N FTRS +P II P ++ Q+
Sbjct: 26 DDLPSCLILHGVSNYTTPFISNSDYDRLLYVSMQNQIFTRSTFPRPSVIILPESKDQLVN 85
Query: 123 AIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
I C R GSWTIR+R GGHSYEGLS TA++PFVIIDMMNL+
Sbjct: 86 TISCCRRGSWTIRLRSGGHSYEGLSHTADSPFVIIDMMNLN 126
>XP_011626556.1 PREDICTED: reticuline oxidase [Amborella trichopoda]
Length = 524
Score = 105 bits (261), Expect = 8e-24
Identities = 50/99 (50%), Positives = 66/99 (66%), Gaps = 2/99 (2%)
Frame = -3
Query: 291 LLSCLSSGGVANVTTYCSPS--YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVSTAI 118
LL C G+ N TT+ S Y +LLN S +NLRF + S P+ I+ P++ QV A+
Sbjct: 35 LLHCFKGNGLTNYTTFTSTPQIYYKLLNFSLQNLRFAEPSTSMPYVIVLPSSNIQVQRAV 94
Query: 117 RCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
C+R+ W IR RCGGHSYEG SST ++PFVIID+MNL+
Sbjct: 95 VCSRQHWWLIRTRCGGHSYEGFSSTGDSPFVIIDLMNLN 133
>ERN14600.1 hypothetical protein AMTR_s00038p00162870 [Amborella trichopoda]
Length = 568
Score = 105 bits (261), Expect = 1e-23
Identities = 50/99 (50%), Positives = 66/99 (66%), Gaps = 2/99 (2%)
Frame = -3
Query: 291 LLSCLSSGGVANVTTYCSPS--YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVSTAI 118
LL C G+ N TT+ S Y +LLN S +NLRF + S P+ I+ P++ QV A+
Sbjct: 46 LLHCFKGNGLTNYTTFTSTPQIYYKLLNFSLQNLRFAEPSTSMPYVIVLPSSNIQVQRAV 105
Query: 117 RCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
C+R+ W IR RCGGHSYEG SST ++PFVIID+MNL+
Sbjct: 106 VCSRQHWWLIRTRCGGHSYEGFSSTGDSPFVIIDLMNLN 144
>3FW7_A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant
Length = 498
Score = 104 bits (260), Expect = 1e-23
Identities = 52/103 (50%), Positives = 72/103 (69%), Gaps = 3/103 (2%)
Frame = -3
Query: 300 GEDLLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
G DLLSCL+ GV N T + + S + + L+ S +N F S +SKP II P ++ ++
Sbjct: 2 GNDLLSCLTFNGVRNHTVFSADSDSDFNRFLHLSIQNPLFQNSLISKPSAIILPGSKEEL 61
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
S IRC R+GSWTIR+R GG SYEGLS T++TPF++ID+MNL+
Sbjct: 62 SNTIRCIRKGSWTIRLRSGGASYEGLSYTSDTPFILIDLMNLN 104
>ACO90226.1 putative berberine bridge enzyme, partial [Papaver bracteatum]
Length = 259
Score = 100 bits (250), Expect = 2e-23
Identities = 52/99 (52%), Positives = 68/99 (68%), Gaps = 3/99 (3%)
Frame = -3
Query: 291 LLSCLSSGGVANVTTYCSPS---YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQVSTA 121
LLSCL+S V N TT + + Y +LL+ S +N F + VSKP I+ P ++ ++S+
Sbjct: 22 LLSCLNSHDVHNFTTLSTDTNSEYLKLLHASIQNPLFAKPTVSKPSFIVIPGSKEELSST 81
Query: 120 IRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNL 4
I C SWTIR+R GGHSYEGLS TA+TPFVI+DMMNL
Sbjct: 82 IHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNL 120
>AAU20769.1 berberine bridge enzyme [Thalictrum flavum subsp. glaucum]
Length = 535
Score = 101 bits (252), Expect = 2e-22
Identities = 54/103 (52%), Positives = 67/103 (65%), Gaps = 4/103 (3%)
Frame = -3
Query: 297 EDLLSCLSSGGVANVTTYCSPS----YPQLLNTSTKNLRFTRSNVSKPFTIIFPTNQSQV 130
+DL SCL+ GV N TT+ S S Y +LL S +N FTRS +P II P + Q+
Sbjct: 21 DDLPSCLTIHGVHNYTTHQSTSNSDAYHRLLYVSMQNQIFTRSTFPQPRVIILPESMDQL 80
Query: 129 STAIRCAREGSWTIRVRCGGHSYEGLSSTANTPFVIIDMMNLD 1
+ I C GSWTIR+R GGHSYEGLS A+ PFVIID+MNL+
Sbjct: 81 ANVISCCTRGSWTIRLRSGGHSYEGLSHIADNPFVIIDLMNLN 123
>KCW70998.1 hypothetical protein EUGRSUZ_F04104 [Eucalyptus grandis]
Length = 493
Score = 101 bits (251), Expect = 2e-22
Identities = 53/110 (48%), Positives = 70/110 (63%), Gaps = 7/110 (6%)
Frame = -3
Query: 309 CACGEDLLSCLSSGGVANVTTYC-----SPSYPQLLNTSTKNLRFTRSNVSKPFTIIFPT 145
C+ + + SCL+ V N TT+ S Y +LLN+S NLRF+ V KP IIFP
Sbjct: 9 CSSADSIASCLTQHNVNNFTTFPATENESARYYELLNSSIHNLRFSGPAVPKPVAIIFPG 68
Query: 144 NQSQVSTAIRCAREGSWTIRVRCGGHSYEGLS--STANTPFVIIDMMNLD 1
+ Q+ ++I C REG+W IRVR GGHSYEG S +T PFV++DMMNL+
Sbjct: 69 SVEQLKSSIVCCREGAWEIRVRSGGHSYEGTSWVATDGAPFVVVDMMNLN 118
>XP_010063741.1 PREDICTED: reticuline oxidase [Eucalyptus grandis]
Length = 551
Score = 101 bits (251), Expect = 2e-22
Identities = 53/110 (48%), Positives = 70/110 (63%), Gaps = 7/110 (6%)
Frame = -3
Query: 309 CACGEDLLSCLSSGGVANVTTYC-----SPSYPQLLNTSTKNLRFTRSNVSKPFTIIFPT 145
C+ + + SCL+ V N TT+ S Y +LLN+S NLRF+ V KP IIFP
Sbjct: 27 CSSADSIASCLTQHNVNNFTTFPATENESARYYELLNSSIHNLRFSGPAVPKPVAIIFPG 86
Query: 144 NQSQVSTAIRCAREGSWTIRVRCGGHSYEGLS--STANTPFVIIDMMNLD 1
+ Q+ ++I C REG+W IRVR GGHSYEG S +T PFV++DMMNL+
Sbjct: 87 SVEQLKSSIVCCREGAWEIRVRSGGHSYEGTSWVATDGAPFVVVDMMNLN 136