BLASTX nr result
ID: Magnolia22_contig00023265
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Magnolia22_contig00023265
(1047 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 476 e-164
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 471 e-162
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 441 e-150
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 438 e-149
AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum fla... 437 e-148
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 436 e-148
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 431 e-146
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 428 e-145
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 424 e-144
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik... 419 e-142
AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum] 336 e-109
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 333 e-108
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 328 e-106
XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 313 e-100
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 306 2e-97
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 306 2e-97
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 303 5e-96
XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 302 6e-96
CDP15158.1 unnamed protein product [Coffea canephora] 301 1e-95
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 300 1e-94
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 476 bits (1225), Expect = e-164
Identities = 233/352 (66%), Positives = 280/352 (79%), Gaps = 3/352 (0%)
Frame = +1
Query: 1 MEFLSIALL-FSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSY 177
ME +SIA++ FSF+ +F+LRDPRQK LPPGPRP PI+GNLLQLGD P ++ AKLAQ Y
Sbjct: 1 MEVVSIAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G LF+LKLG+QT E+LKTHD++ SGRYVF +FR++ HVE S+VWSECND
Sbjct: 61 GQLFSLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK+LR++CRTELF+PKMIE Q+ +RE K +MV L KEGEVVKI E+VFGTL NIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGN 180
Query: 538 LIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+SKDVFDL DP+GG E+K +W+L+E+GNSTNPADYFPI+G++DLF QRRAVAD L
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQ 240
Query: 712 QIYDVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEW 891
QIYDVW L+ERR K ++SN+DF +VLL A L D +INALLME+FG GTETSASTIEW
Sbjct: 241 QIYDVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEW 300
Query: 892 AMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
A+ EL K P ++SK+ EL VVG VKESDLP LPYLQA VKETLRLHPP
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPP 352
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 471 bits (1213), Expect = e-162
Identities = 232/352 (65%), Positives = 280/352 (79%), Gaps = 3/352 (0%)
Frame = +1
Query: 1 MEFLSIALL-FSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSY 177
ME LSIA++ FSF+ +F+LRD R K LPPGPRP PI+GNLLQLGD PH++ AKLAQ Y
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G LF+LKLG+QT EILKTHD++ SGRYVF +FR++ HVE S+VWSECND
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDN 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK+LR++CRTELF+PKMIE Q+ +RE K +MV L KEGEVVKI E+VFGTL NIFGN
Sbjct: 121 WKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGN 180
Query: 538 LIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+SKDVFDL DP+GG E+K +W+L+++GNSTNPADYFPI+G+ DLF QRRAVA+ L
Sbjct: 181 LIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQ 240
Query: 712 QIYDVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEW 891
QIYDVW +L+ERR K ++S +DF +VLL A L+D +INALLME+FGAGTETSASTIEW
Sbjct: 241 QIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEW 300
Query: 892 AMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
A+ EL K P ++SK+ EL VVG VKESDLP LPYLQA VKETLRLHPP
Sbjct: 301 AITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPP 352
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 441 bits (1133), Expect = e-150
Identities = 213/355 (60%), Positives = 273/355 (76%), Gaps = 6/355 (1%)
Frame = +1
Query: 1 MEFLSIALLFSFIC-IAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSY 177
ME +++AL+ I I Y L K LPPGP+PWPI+GNLLQLG+ PH++ A+LAQ+Y
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G +FTLK+G +T EILKTHDR+ S RYVF +FR++ HVE S+VWS+C +
Sbjct: 61 GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK LR++CRTELF+ KMIE QAH+RE K +MV +L +K+GE VKI E++FGTL NIFGN
Sbjct: 121 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180
Query: 538 LIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+S+++F+L DP+ G E K +WR++ELGNSTNPADYFP+LG+FDLF QR+ VA+CL
Sbjct: 181 LIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240
Query: 712 QIYDVWNAILRERREKKEAD---SNHDFTNVLLEANLNDHQINALLMEMFGAGTETSAST 882
IY +W A+L+ER+ K+ D S +DF +V L++ LND+QINALLME+FGAGTETSAST
Sbjct: 241 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 300
Query: 883 IEWAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
IEWAM EL KNP I +K+ SEL VVG + VKESD P+LPYL+A VKETLRLHPP
Sbjct: 301 IEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPP 355
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 438 bits (1126), Expect = e-149
Identities = 212/355 (59%), Positives = 272/355 (76%), Gaps = 6/355 (1%)
Frame = +1
Query: 1 MEFLSIALLFSFIC-IAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSY 177
ME +++AL+ I I Y L K LPPGP+PWPI+GNLLQLG+ PH++ A+LAQ+Y
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G +FTLK+G +T EILKTHDR+ S RYVF +FR++ HVE S+VWS+C +
Sbjct: 61 GDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTET 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK LR++CRTELF+ KMIE QAH+RE K +MV +L +K+GE VKI E++FGTL NIFGN
Sbjct: 121 WKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGN 180
Query: 538 LIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+S+++F+L P+ G E K +WR++ELGNSTNPADYFP+LG+FDLF QR+ VA+CL
Sbjct: 181 LIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLK 240
Query: 712 QIYDVWNAILRERREKKEAD---SNHDFTNVLLEANLNDHQINALLMEMFGAGTETSAST 882
IY +W A+L+ER+ K+ D S +DF +V L++ LND+QINALLME+FGAGTETSAST
Sbjct: 241 GIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAST 300
Query: 883 IEWAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
IEWAM EL KNP I +K+ SEL VVG + VKESD P+LPYL+A VKETLRLHPP
Sbjct: 301 IEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPP 355
>AAU20767.1 (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 437 bits (1123), Expect = e-148
Identities = 221/353 (62%), Positives = 267/353 (75%), Gaps = 4/353 (1%)
Frame = +1
Query: 1 MEFLSIAL--LFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQS 174
ME LS A+ LF +F+L D R K LPPGPRP PI+GNLLQLG+ PH++ AKLA+
Sbjct: 1 MEVLSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEK 60
Query: 175 YGHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECND 354
YG LFTLKLG+QT EILKT D++ SGRYVF +FR+ HV S+VWSECN+
Sbjct: 61 YGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNE 120
Query: 355 YWKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFG 534
WK+LR++CRTELFSPKMIE QA++RE K DMV L +KE + VKI E+VF TL NIFG
Sbjct: 121 NWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFG 180
Query: 535 NLIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCL 708
NLI+SKDVFDL DP+GG E+K +W+L+++GNSTNPADYFPI+G+FDLF QRR VA L
Sbjct: 181 NLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVL 240
Query: 709 HQIYDVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIE 888
QIYDVW +L+ERR N DF NVLL A L+D +INALLME+FGAGTET+ASTIE
Sbjct: 241 KQIYDVWGVMLKERRSITGHREN-DFANVLLNAGLDDQKINALLMELFGAGTETTASTIE 299
Query: 889 WAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
WA+ EL N ++SK+ +EL VVG K VKES++P LPYLQA VKETLRLHPP
Sbjct: 300 WAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPP 352
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 436 bits (1122), Expect = e-148
Identities = 210/349 (60%), Positives = 269/349 (77%), Gaps = 5/349 (1%)
Frame = +1
Query: 16 IALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTL 195
IA++ S I Y L K LPPGP+PWPI+GNLLQLG+ PH++ A+LAQ+YG +FTL
Sbjct: 8 IAVIIS--SILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTL 65
Query: 196 KLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYWKMLRR 375
K+G +T EILKTHDR+ S RYVF +FR++ HVE S+VWS+C + WK LR+
Sbjct: 66 KMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRK 125
Query: 376 ICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKD 555
+CRTELF+ KMIE QAH+RE K +MV +L +K+GE VKI E++FGTL NIFGNLI+S++
Sbjct: 126 VCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQN 185
Query: 556 VFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVW 729
+F+L DP+ G E K +WR++ELGNSTNPADYFP+LG+FDLF QR+ VA+CL IY +W
Sbjct: 186 IFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 245
Query: 730 NAILRERREKKEAD---SNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMA 900
A+L+ER+ K+ D S +DF +V L++ LND+QINALLME+FGAGTETSASTIEWAM
Sbjct: 246 GAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 305
Query: 901 ELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
EL KNP I +K+ SE+ VVG + VKESD P+LPYL+A VKETLRLHPP
Sbjct: 306 ELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPP 354
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 431 bits (1107), Expect = e-146
Identities = 208/336 (61%), Positives = 259/336 (77%), Gaps = 4/336 (1%)
Frame = +1
Query: 49 YFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTLKLGAQTXXXXX 228
Y + RD KGLPPGP+PWPI+GNLLQLG+ PHS+ A+LA++YG LFTLKLG++T
Sbjct: 17 YLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVAS 76
Query: 229 XXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYWKMLRRICRTELFSPKM 408
EILKTHDRV SGRYVF +FR++ HVE S+VWSECN+ WK LR++CR ELF+ KM
Sbjct: 77 TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKM 136
Query: 409 IEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKDVFDLSDPSGG- 585
IE QA +RE+K +MV L R +G VKI E+VFGTL NIFGNLI+S+++F L D S G
Sbjct: 137 IESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 196
Query: 586 -EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVWNAILRERREKK 762
E+K +WR++ELGNSTNPADYFP LG FDLF QR+ VADCL IY VW A+L+ER+ K
Sbjct: 197 VEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAK 256
Query: 763 --EADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAELIKNPDIMSKV 936
+DF +LL++ L+D QINALLME+FGAGTETSASTIEWA++EL KNP++ + +
Sbjct: 257 LHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANM 316
Query: 937 LSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHP 1044
SEL VVG + VKESD+P++PYLQA VKETLRLHP
Sbjct: 317 RSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHP 352
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 428 bits (1100), Expect = e-145
Identities = 206/336 (61%), Positives = 263/336 (78%), Gaps = 4/336 (1%)
Frame = +1
Query: 49 YFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTLKLGAQTXXXXX 228
Y + RD KGLPPGP+PWPI+GNLLQLG+ PHS+ A+LA++YG LF+LKLG++T
Sbjct: 12 YLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVAS 71
Query: 229 XXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYWKMLRRICRTELFSPKM 408
EILKTHDRV SGRYVF +FR++ HVE S+VWSECN+ WK LR++CRTELF+ KM
Sbjct: 72 TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKM 131
Query: 409 IEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKDVFDLSDPSGG- 585
IE QA +RE+K +MV +L + G VKIAE+VFGTL NIFGNLI+S+++F L D S G
Sbjct: 132 IESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 191
Query: 586 -EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVWNAILRERREKK 762
E+K +WR++ELGNSTNPADYFP LG+FDLF Q + VADCL IY VW A+L+ER+ K
Sbjct: 192 VEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAK 251
Query: 763 EADSN--HDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAELIKNPDIMSKV 936
+ +++ +DF +LL++ L+D QINALLME+FGAGTETSASTIEWA++EL KNP + + +
Sbjct: 252 QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANM 311
Query: 937 LSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHP 1044
EL VVG + VKESD+P++PYLQA VKETLRLHP
Sbjct: 312 RLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHP 347
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 424 bits (1091), Expect = e-144
Identities = 204/336 (60%), Positives = 262/336 (77%), Gaps = 4/336 (1%)
Frame = +1
Query: 49 YFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTLKLGAQTXXXXX 228
Y + RD KGLPPGP+PWPI+GNLLQLG+ PHS+ A+LA++YG LF+LKLG++T
Sbjct: 12 YLIFRDSSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVAS 71
Query: 229 XXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYWKMLRRICRTELFSPKM 408
EILKTHDRV SGRYVF +FR++ HVE S+VWSECN+ WK LR++CRT+LF+ KM
Sbjct: 72 TPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKM 131
Query: 409 IEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKDVFDLSDPSGG- 585
IE QA +RE+K +MV +L + G VKIAE+VFGTL NIFGNLI+S+++F L D S G
Sbjct: 132 IESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGS 191
Query: 586 -EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVWNAILRERREKK 762
E+K +WR++ELGNSTNPADYFP LG+FDLF Q + VADCL IY VW A+L+E + K
Sbjct: 192 VEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAK 251
Query: 763 EADSN--HDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAELIKNPDIMSKV 936
+ +++ +DF +LL++ L+D QINALLME+FGAGTETSASTIEWA++EL KNP + + +
Sbjct: 252 QHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANM 311
Query: 937 LSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHP 1044
EL VVG + VKESD+P++PYLQA VKETLRLHP
Sbjct: 312 RLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHP 347
>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
P450 [Papaver somniferum]
Length = 440
Score = 419 bits (1078), Expect = e-142
Identities = 203/355 (57%), Positives = 263/355 (74%), Gaps = 6/355 (1%)
Frame = +1
Query: 1 MEFLSIALL-FSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSY 177
ME +++AL+ F Y ++R+ GLPPGP+PWPI+GNLLQLG+ PHS+ A+LA++Y
Sbjct: 1 MEIVTVALIAIVFTTFLYLIVRESSPNGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G LFTLKLG QT E+LK HDR GRYVF +FR++NHVE S+VW+ECN+
Sbjct: 61 GDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKNHVENSIVWNECNET 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK LR++CRT+LF+ KMIE QA +RE K +MV +L + EG VKI E++FGTL N+FGN
Sbjct: 121 WKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGN 180
Query: 538 LIYSKDVFDLSDPSGG--EIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+S+D+F L D S G E+K IWR++ELGNS NP DY P+LG DLF QR+ VADCL
Sbjct: 181 LIFSQDIFKLGDESSGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQ 240
Query: 712 QIYDVWNAILRERREKK---EADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSAST 882
+Y VW A+L+ERR K D+ +DF +VLL++ L+D QIN+LL++MF AGTET+AST
Sbjct: 241 GVYGVWGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTAST 300
Query: 883 IEWAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
IEWA+ EL KNP + + + SEL VVG + VKESD+ +LPYLQA VKETLRLHPP
Sbjct: 301 IEWALTELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPP 355
>AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 336 bits (861), Expect = e-109
Identities = 167/348 (47%), Positives = 238/348 (68%), Gaps = 3/348 (0%)
Frame = +1
Query: 10 LSIALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMP---HSKLAKLAQSYG 180
L+ ALL+ I ++L PR K LPPGP+PWPI+GN+ L H L +A+++G
Sbjct: 3 LTTALLYLIPTILLYILFKPRSKNLPPGPKPWPIIGNIPALFSSAYPMHVALTNMARTHG 62
Query: 181 HLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYW 360
+ L LG Q EILKTHDR FSGR V +FR+++H++YS+VW++C++YW
Sbjct: 63 PIMILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYW 122
Query: 361 KMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNL 540
K+LR+I RTE+FSPKM+++Q H+RE KV+++V L KEG+VVKI+++VFGTL NI GN+
Sbjct: 123 KLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNV 182
Query: 541 IYSKDVFDLSDPSGGEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIY 720
I+SKDVF G +++ I ++ +G + N A+++PI EFDL ++ ++
Sbjct: 183 IFSKDVFRYVGADGDKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVM 242
Query: 721 DVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMA 900
+W + ER+ K+ + D +VLL + ND QINA+ +E FG G+ETS++T EWA+A
Sbjct: 243 KMWKGTVEERKVKRNEEPK-DMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALA 301
Query: 901 ELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHP 1044
ELIKNP I++KV ELD+VVG VKESDLP LPYLQAC+KET+RLHP
Sbjct: 302 ELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRLHP 349
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 333 bits (854), Expect = e-108
Identities = 175/353 (49%), Positives = 239/353 (67%), Gaps = 9/353 (2%)
Frame = +1
Query: 16 IALLFSFI-----CIAYFLLRDPRQKGLPPGPRPWPILGNLL-QLGDMPHSKLAKLAQSY 177
+ALL SFI ++ L P LPPGP WPI+G LL +L PH +L+KLAQ +
Sbjct: 1 MALLVSFIFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRF 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G L LK G + E+LK DR+ SGR+ H+ RI+ ++E+SMVW++C DY
Sbjct: 61 GPLMLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDY 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WKM+R++ RTELFS KM+++QAH RE KVS+++ L RKEGE V A+++FG++ NI G
Sbjct: 121 WKMVRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGA 180
Query: 538 LIYSKDVFDLSDPSGGEI--KGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
LI+SKDV+D D + + KG I +LM L N AD +PILG D R+A A C+
Sbjct: 181 LIFSKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVK 240
Query: 712 QIYDVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEW 891
++ + W AI+++RR K + S +DF VLL++ +D QI+A+L+E FG G++TS STIEW
Sbjct: 241 RMNESWAAIVKQRR-KNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEW 299
Query: 892 AMAELIKNPDIMSKVLSELDKVV-GSKMVKESDLPSLPYLQACVKETLRLHPP 1047
AMAEL++NP+ + KV ELD+V+ S VKESDLP+LPYL ACVKETLRLHPP
Sbjct: 300 AMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPP 352
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 328 bits (841), Expect = e-106
Identities = 164/347 (47%), Positives = 238/347 (68%), Gaps = 7/347 (2%)
Frame = +1
Query: 25 LFSFI--CIAYFLLRDPRQKGLPPGPRPWPILGNLLQL---GDMP-HSKLAKLAQSYGHL 186
LFS I + + LL P+ K LPPGP PWP++GNL L ++P H LA +A+++G +
Sbjct: 7 LFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPI 66
Query: 187 FTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDYWKM 366
L LG Q EILKTHDR+FS R++ +FR+++H++YS+VWS+C DYWK+
Sbjct: 67 MILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKL 126
Query: 367 LRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIY 546
LR+I RTE+FSPKM++ Q+H+RE KV++++ L KEG+VVKI++ VFGTL NI GN+++
Sbjct: 127 LRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVF 186
Query: 547 SKDVFDLSDPSG-GEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYD 723
SKDVF D + G I+ I ++ +G N A+++P L E DL ++ + ++
Sbjct: 187 SKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMK 246
Query: 724 VWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAE 903
+W ++ER+ + +S D +VLL + ND QINAL +E FG G+ETS++TIEW +AE
Sbjct: 247 MWEGTVKERKANRNEESK-DMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAE 305
Query: 904 LIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHP 1044
LIK+P M+KV EL++VVG+ +KESDLP LPYLQAC+KE +RLHP
Sbjct: 306 LIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHP 352
>XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 313 bits (802), Expect = e-100
Identities = 163/354 (46%), Positives = 232/354 (65%), Gaps = 5/354 (1%)
Frame = +1
Query: 1 MEFLSIALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDM-PHSKLAKLAQSY 177
M L++ +LF ++ L P K LPPGP WPI+G L M P+ +L KLAQ Y
Sbjct: 1 MALLALFILFLLSILSLVLFLKPSSKKLPPGPFSWPIIGTQLPSPTMKPNLELFKLAQRY 60
Query: 178 GHLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHVEYSMVWSECNDY 357
G L + G + E+LK DRV SGR+ ++ R++ ++EYSMVW++C DY
Sbjct: 61 GPLMLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDY 120
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WKM+R+I RTELFS KM+++ AH+RE KVS+++ L RKEGE V +++FG + N+ G
Sbjct: 121 WKMVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGA 180
Query: 538 LIYSKDVFDLSDPSGGEI--KGQIWRLMELGNSTNPADYFPILGEFDLFR-QRRAVADCL 708
LIYSKDV+D D + + KG I +LM L + N AD +PI + F+ R+ A C+
Sbjct: 181 LIYSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACV 240
Query: 709 HQIYDVWNAILRERREKKEADSNHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIE 888
++ + W AI+ ERR+ + N D VLL++ +D QI+A+ +E FG G++TSASTIE
Sbjct: 241 KRMSESWAAIINERRKNNDHTKN-DLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIE 299
Query: 889 WAMAELIKNPDIMSKVLSELDKVVG-SKMVKESDLPSLPYLQACVKETLRLHPP 1047
WA+AEL++NP+ + K+ ELD+V+G + VK+SDLP+LPYL ACVKETLRLHPP
Sbjct: 300 WALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVKETLRLHPP 353
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 306 bits (785), Expect = 2e-97
Identities = 167/353 (47%), Positives = 218/353 (61%), Gaps = 6/353 (1%)
Frame = +1
Query: 7 FLSIALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHL 186
FL LL F+ I + P LPPGP PWPI+GNLLQ+G PH+KLA LA+ +G L
Sbjct: 15 FLYALLLLPFLVILKHIFLKPPP--LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPL 72
Query: 187 FTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHV--EYSMVWS-ECNDY 357
+L+LG Q E+LKTHDR SGRY+ + ++N S+V++ +CN
Sbjct: 73 MSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTN 132
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK LR ICR ELFS K +E Q LRE KV+++V L KEGEVVK+ ++VF T+ NI N
Sbjct: 133 WKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSN 192
Query: 538 LIYSKDVFDLSDPS--GGEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
+S D+ D D GG +K I + E G + N +DY+PILG D+ R +
Sbjct: 193 KFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFE 252
Query: 712 QIYDVWNAILRERREKKEADSNH-DFTNVLLEANLNDHQINALLMEMFGAGTETSASTIE 888
+I W IL+ERR ++ S+H DF LLE D QIN +++E+F AG ETS+ T+E
Sbjct: 253 RIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVE 312
Query: 889 WAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
WAMAELI+N D M K+ EL ++VG V+ES LP LPYLQACVKE LRLHPP
Sbjct: 313 WAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPP 365
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 306 bits (785), Expect = 2e-97
Identities = 167/353 (47%), Positives = 218/353 (61%), Gaps = 6/353 (1%)
Frame = +1
Query: 7 FLSIALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHL 186
FL LL F+ I + P LPPGP PWPI+GNLLQ+G PH+KLA LA+ +G L
Sbjct: 15 FLYALLLLPFLVILKHIFLKPPP--LPPGPYPWPIIGNLLQMGKNPHAKLANLAKLHGPL 72
Query: 187 FTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIENHV--EYSMVWS-ECNDY 357
+L+LG Q E+LKTHDR SGRY+ + ++N S+V++ +CN
Sbjct: 73 MSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTN 132
Query: 358 WKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGN 537
WK LR ICR ELFS K +E Q LRE KV+++V L KEGEVVK+ ++VF T+ NI N
Sbjct: 133 WKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSN 192
Query: 538 LIYSKDVFDLSDPS--GGEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLH 711
+S D+ D D GG +K I + E G + N +DY+PILG D+ R +
Sbjct: 193 KFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFE 252
Query: 712 QIYDVWNAILRERREKKEADSNH-DFTNVLLEANLNDHQINALLMEMFGAGTETSASTIE 888
+I W IL+ERR ++ S+H DF LLE D QIN +++E+F AG ETS+ T+E
Sbjct: 253 RIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVE 312
Query: 889 WAMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
WAMAELI+N D M K+ EL ++VG V+ES LP LPYLQACVKE LRLHPP
Sbjct: 313 WAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPP 365
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 303 bits (775), Expect = 5e-96
Identities = 152/331 (45%), Positives = 217/331 (65%), Gaps = 5/331 (1%)
Frame = +1
Query: 70 RQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTLKLGAQTXXXXXXXXXXXE 249
+++ LPPGPR WPILGN+LQ+G PH +A A+ +G L +L+LG + E
Sbjct: 33 KRRPLPPGPRQWPILGNILQVGKNPHISMANYAKVHGPLISLRLGTRVVVVASSPTAAAE 92
Query: 250 ILKTHDRVFSGRYVFHNFRIENHV--EYSMVWS-ECNDYWKMLRRICRTELFSPKMIEMQ 420
ILKTHDR+ SGRY+ E++V ++VW+ C+D WK LR +CR+ELFS K IE Q
Sbjct: 93 ILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKAIESQ 152
Query: 421 AHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKDVFDLSDP-SGGEIKG 597
A LRE K+++M+ L K+G++V I E+VF T FN NL++SKD+ D + GE+K
Sbjct: 153 ATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFEDQGNAGELKT 212
Query: 598 QIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVWNAILRERREKKEADSN 777
I LMEL N AD++P+L + D +R + +CL +++ VW+ ++ERRE+ D+
Sbjct: 213 LISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVKDAR 272
Query: 778 H-DFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAELIKNPDIMSKVLSELDK 954
DF +V L +DHQIN LL+E+F AG +T+ +T+EWAMAEL+K ++ KV EL+
Sbjct: 273 KTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELET 332
Query: 955 VVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
+G M++ES +P L YL ACVKETLRLHPP
Sbjct: 333 EIGKDMIRESHIPQLKYLNACVKETLRLHPP 363
>XP_011041604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Populus euphratica]
Length = 492
Score = 302 bits (774), Expect = 6e-96
Identities = 152/331 (45%), Positives = 212/331 (64%), Gaps = 5/331 (1%)
Frame = +1
Query: 70 RQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHLFTLKLGAQTXXXXXXXXXXXE 249
+ + LPPGP+PWPI+GN+L G PH A A+++G L +LKLG Q E
Sbjct: 29 KNQSLPPGPKPWPIIGNILHFGKKPHISTANFAKTHGPLISLKLGKQLLIVGSSKRAATE 88
Query: 250 ILKTHDRVFSGRYVFHNFRIENHV--EYSMVWS-ECNDYWKMLRRICRTELFSPKMIEMQ 420
ILK+HDR+ S RYVF ENHV S+VW+ +C+D WK LR +CR ELFS K IE Q
Sbjct: 89 ILKSHDRLLSARYVFKAALFENHVLDRISIVWATQCSDGWKSLRALCRNELFSAKAIESQ 148
Query: 421 AHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFGNLIYSKDVFDLSDPSGGE-IKG 597
A LRE K+ +MV +GR++GEVV I E+V +FN NL++S D+ L D +K
Sbjct: 149 AVLREKKMGEMVEFIGRRKGEVVGIGEVVLAIVFNTIANLLFSVDLIGLEDDGAATGLKS 208
Query: 598 QIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQIYDVWNAILRERREKKEADS- 774
+WR+M+LG + N +D++PILG D +R +A C++Q++ +W ++ERREK D
Sbjct: 209 LMWRMMKLGATPNISDFYPILGGIDPQGLKRKMAVCVNQMFGIWGKYIKERREKHVHDGP 268
Query: 775 NHDFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEWAMAELIKNPDIMSKVLSELDK 954
DF +V L D QIN L +E+ AGT+T+A+T+EWA+AEL+KN +++ KV E+ +
Sbjct: 269 RSDFLDVFLANGFEDLQINWLALELLSAGTDTTATTVEWAIAELLKNKEVLKKVSEEIKR 328
Query: 955 VVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
+ +KES + LPYL ACV ETLRLHPP
Sbjct: 329 ETDTNSLKESHVSQLPYLNACVNETLRLHPP 359
>CDP15158.1 unnamed protein product [Coffea canephora]
Length = 496
Score = 301 bits (772), Expect = 1e-95
Identities = 165/359 (45%), Positives = 226/359 (62%), Gaps = 20/359 (5%)
Frame = +1
Query: 31 SFICIAYFLLRD----------PRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYG 180
SFI + FLL R + LPPGP+PWPI+GNL Q+G PH LA+LAQ YG
Sbjct: 7 SFIFLPLFLLPPLFWLILNYIKSRNQSLPPGPKPWPIVGNLPQIGSKPHVALAQLAQDYG 66
Query: 181 HLFTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFH----NFRIENHVEYSMVWS-E 345
L +L+LG+Q EILKTHDR+ SGR+V H + N+V S+ W+ E
Sbjct: 67 PLISLRLGSQLVVVGSTPAAATEILKTHDRILSGRHVPHVSYAKSPLMNYV--SVGWTYE 124
Query: 346 CNDYWKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFN 525
C D WK LR +C++E+ K+IE Q+HLRE K +++V L KEG+ +KIAE+VF ++FN
Sbjct: 125 CTDQWKFLRTLCKSEILGAKVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFN 184
Query: 526 IFGNLIYSKDVFDLSD-PSGGEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVAD 702
G + +SKD + +GG + I +MEL + N +D +P+LG DL R + +
Sbjct: 185 FLGQIFFSKDFLSYDEVENGGGMSELIREVMELWTAPNISDLYPVLGGLDLQRLSKKASV 244
Query: 703 CLHQIYDVWNAILRERREKKEADSNH--DFTNVLLEANLNDHQINALLMEMFGAGTETSA 876
C ++I W I+RERR KK DS DF +VLL+ + +D QIN L +E+F AG++TS
Sbjct: 245 CHNKICSAWQEIIRERRGKKYQDSTRQKDFLDVLLQNDFSDDQINYLHLELFAAGSDTST 304
Query: 877 STIEWAMAELIKNPDIMSKVLSELDKVVGS--KMVKESDLPSLPYLQACVKETLRLHPP 1047
ST+EWAMAEL++NP + + ELD++ S ++KESDL LPYLQACVKE LRLHPP
Sbjct: 305 STVEWAMAELLRNPKFLDMIRQELDQIEYSVENVIKESDLTRLPYLQACVKEILRLHPP 363
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 300 bits (767), Expect = 1e-94
Identities = 160/352 (45%), Positives = 213/352 (60%), Gaps = 5/352 (1%)
Frame = +1
Query: 7 FLSIALLFSFICIAYFLLRDPRQKGLPPGPRPWPILGNLLQLGDMPHSKLAKLAQSYGHL 186
F+ LL F+ I + P LPPGP PWPI+GNLLQLG PH KLA LA+ +G L
Sbjct: 15 FVYALLLLPFLLILKHIFLKPPP--LPPGPYPWPIIGNLLQLGKNPHVKLASLAKLHGPL 72
Query: 187 FTLKLGAQTXXXXXXXXXXXEILKTHDRVFSGRYVFHNFRIE----NHVEYSMVWSECND 354
+L+LG Q E+LKTHDR SGRYV + ++ NH+ + EC +
Sbjct: 73 MSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFA-KECTN 131
Query: 355 YWKMLRRICRTELFSPKMIEMQAHLRENKVSDMVGHLGRKEGEVVKIAEIVFGTLFNIFG 534
WK LR ICRTE+FS K +E LRE KV ++V L KEGEVVK+ ++VF T+ NI
Sbjct: 132 NWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILS 191
Query: 535 NLIYSKDVFDLSDPSGGEIKGQIWRLMELGNSTNPADYFPILGEFDLFRQRRAVADCLHQ 714
N +S D+ D +K I+R ELG + N +D++PIL +L ++ + L +
Sbjct: 192 NTFFSMDLCDFEREG---LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGR 248
Query: 715 IYDVWNAILRERREKKEADSNH-DFTNVLLEANLNDHQINALLMEMFGAGTETSASTIEW 891
I W L+ERR++K S+H D LE D QIN +++E+F AG +TS TIEW
Sbjct: 249 ILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEW 308
Query: 892 AMAELIKNPDIMSKVLSELDKVVGSKMVKESDLPSLPYLQACVKETLRLHPP 1047
A+ +LI+NPD+M K+ EL K++G V+ES LP LPYLQACVKETLRLHPP
Sbjct: 309 AITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPP 360