BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Magnolia22_contig00019378
         (536 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

JAT61810.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Anth...   189   2e-54
JAT56798.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1, part...   184   1e-52
JAT44910.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1, part...   179   1e-50
KDP20161.1 hypothetical protein JCGZ_00026 [Jatropha curcas] KDP...   171   3e-48
XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ...   172   4e-48
XP_012076022.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd...   171   9e-48
XP_010934155.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ...   167   1e-47
OAY59868.1 hypothetical protein MANES_01G066500 [Manihot esculenta]   170   2e-47
CDP15158.1 unnamed protein product [Coffea canephora]                 169   6e-47
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd...   168   9e-47
XP_015896286.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ...   168   2e-46
XP_015878021.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd...   167   2e-46
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox...   167   3e-46
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso...   166   4e-46
I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-lik...   165   6e-46
XP_010112567.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [...   166   9e-46
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso...   165   1e-45
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from...   165   1e-45
XP_002274586.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd...   165   1e-45
XP_009610421.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ...   164   3e-45

>JAT61810.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Anthurium
           amnicola]
          Length = 503

 Score =  189 bits (479), Expect = 2e-54
 Identities = 95/184 (51%), Positives = 120/184 (65%), Gaps = 6/184 (3%)
 Frame = +2

Query: 2   WDEFIRERRAA--GYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWT 175
           WD  I ERR    G +  +AK DFL +L+ +G +DL +KAL  D+F A TDT+ TLIEWT
Sbjct: 254 WDGVIGERRKEMEGGERREAKEDFLKLLVESGCDDLQLKALFLDIFTAGTDTSATLIEWT 313

Query: 176 MSELLRNPKAMRRVRDEFEMVVGAENG----VNESHLNHLNYLDACIKESXXXXXXXXXX 343
           M+EL++NP  M RVRDE   ++G+ N     + ESHL HL+YL  CIKE+          
Sbjct: 314 MAELVKNPHVMIRVRDELNQIIGSSNKNMITIRESHLTHLSYLHVCIKETLRLHPPVPLL 373

Query: 344 XXXXXXDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFE 523
                 DT  VM+Y IPKD  VMVNVW+IGRDP +WKDPL F+PE FL+ S+D+KG  FE
Sbjct: 374 LPHRAIDTCQVMDYMIPKDSIVMVNVWSIGRDPKVWKDPLRFMPERFLDCSMDYKGMDFE 433

Query: 524 FTPF 535
           F PF
Sbjct: 434 FVPF 437


>JAT56798.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1, partial [Anthurium
           amnicola]
          Length = 521

 Score =  184 bits (467), Expect = 1e-52
 Identities = 96/185 (51%), Positives = 118/185 (63%), Gaps = 7/185 (3%)
 Frame = +2

Query: 2   WDEFIRERRA---AGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEW 172
           W   +++RR       DGG AK DFL VLL++G  D+ IKAL+ D+F A TDT+TTL EW
Sbjct: 272 WGVVLQQRRVDMERDVDGGGAKEDFLKVLLASGLGDVQIKALMLDIFTAGTDTSTTLTEW 331

Query: 173 TMSELLRNPKAMRRVRDEFEMVVG----AENGVNESHLNHLNYLDACIKESXXXXXXXXX 340
            MSEL++N + M +V DE E V+G      N + ES L HL YL ACIKE+         
Sbjct: 332 AMSELVKNSEVMNKVIDELEGVMGDPKKGNNIILESQLVHLTYLHACIKETLRLHPPAPL 391

Query: 341 XXXXXXXDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHF 520
                  D  ++MNYTIPKDC VMVN WAIGRDP IWKDPL F PE FL+ S+D+KG  +
Sbjct: 392 LLPRHAIDACEMMNYTIPKDCAVMVNAWAIGRDPKIWKDPLVFSPERFLDCSLDYKGADY 451

Query: 521 EFTPF 535
           EF PF
Sbjct: 452 EFIPF 456


>JAT44910.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1, partial [Anthurium
           amnicola]
          Length = 525

 Score =  179 bits (454), Expect = 1e-50
 Identities = 96/189 (50%), Positives = 118/189 (62%), Gaps = 11/189 (5%)
 Frame = +2

Query: 2   WDEFIRERRA---AGYDGGDAKSDFLDVLLSAGYEDLPIKALI----SDMFIAATDTTTT 160
           W   +++RR       DGG AK DFL VLL++G  D+ IKAL+     D+F A TDT+TT
Sbjct: 272 WGVVLQQRRVDMERDVDGGGAKEDFLKVLLASGLGDVQIKALMLSIWQDIFTAGTDTSTT 331

Query: 161 LIEWTMSELLRNPKAMRRVRDEFEMVVG----AENGVNESHLNHLNYLDACIKESXXXXX 328
           L EW MSEL++N + M +V DE E V+G      N + ES L HL YL ACIKE+     
Sbjct: 332 LTEWAMSELVKNSEVMNKVIDELEGVMGDPKKGNNIILESQLVHLTYLHACIKETLRLHP 391

Query: 329 XXXXXXXXXXXDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFK 508
                      D  ++MNYTIPKDC VMVN WAIGRDP IWKDPL F PE FL+ S+D+K
Sbjct: 392 PAPLLLPRHAIDACEMMNYTIPKDCAVMVNAWAIGRDPKIWKDPLVFSPERFLDCSLDYK 451

Query: 509 GNHFEFTPF 535
           G  +EF PF
Sbjct: 452 GADYEFIPF 460


>KDP20161.1 hypothetical protein JCGZ_00026 [Jatropha curcas] KDP34503.1
           hypothetical protein JCGZ_11053 [Jatropha curcas]
          Length = 436

 Score =  171 bits (433), Expect = 3e-48
 Identities = 86/178 (48%), Positives = 115/178 (64%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W+ FI+ERR      G  K DFLDV +S G++D  I  LI ++F A TDTTTT +EW M+
Sbjct: 196 WEIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELFSAGTDTTTTTVEWAMA 255

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           ELL+N +AM +V  E E  +  +N +NES ++   YL ACIKE+                
Sbjct: 256 ELLKNKEAMNKVCQELEREIN-KNSINESQVSQFPYLYACIKETFRLHPPAPFLGHQPL- 313

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T +VMNYTIPKD +++VN+WA+GRDP  W+DPL F PE FLE+S+D KG+ FE  PF
Sbjct: 314 ETCEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLESSLDLKGHDFELLPF 371


>XP_002510830.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Ricinus
           communis] EEF51432.1 (S)-N-methylcoclaurine
           3'-hydroxylase isozyme, putative [Ricinus communis]
          Length = 496

 Score =  172 bits (435), Expect = 4e-48
 Identities = 82/179 (45%), Positives = 120/179 (67%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAA-GYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W+ +I+ERR    +D    K+DFLD+ LS G++D  I  L+ ++  A TDTT+T +EW M
Sbjct: 253 WEIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSVEWAM 312

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +ELL+N +AM++VR+E +  +  +N + ESH++ L YL+AC+KE+               
Sbjct: 313 AELLKNKEAMKKVREELDREIN-KNPIKESHVSQLPYLNACVKETLRLHPPAPFLIPRRA 371

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +  +VMNYTIPKD +V+VNVWAIGRDP +W+DP +F PE FL +S+D KG+ FE  PF
Sbjct: 372 TENCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELIPF 430


>XP_012076022.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Jatropha curcas] XP_012092884.1 PREDICTED: probable
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Jatropha curcas]
          Length = 502

 Score =  171 bits (433), Expect = 9e-48
 Identities = 86/178 (48%), Positives = 115/178 (64%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W+ FI+ERR      G  K DFLDV +S G++D  I  LI ++F A TDTTTT +EW M+
Sbjct: 262 WEIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELFSAGTDTTTTTVEWAMA 321

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           ELL+N +AM +V  E E  +  +N +NES ++   YL ACIKE+                
Sbjct: 322 ELLKNKEAMNKVCQELEREIN-KNSINESQVSQFPYLYACIKETFRLHPPAPFLGHQPL- 379

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T +VMNYTIPKD +++VN+WA+GRDP  W+DPL F PE FLE+S+D KG+ FE  PF
Sbjct: 380 ETCEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLESSLDLKGHDFELLPF 437


>XP_010934155.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Elaeis guineensis]
          Length = 338

 Score =  167 bits (422), Expect = 1e-47
 Identities = 87/180 (48%), Positives = 115/180 (63%), Gaps = 2/180 (1%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKS--DFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWT 175
           W+  I ERR +  +   AKS  DFL VLLSA + D  + AL+ D+F+A TDT TT IEW 
Sbjct: 96  WEGMINERRESKGESIGAKSPHDFLSVLLSAEFSDPQVNALLLDIFVAGTDTATTTIEWV 155

Query: 176 MSELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXX 355
           M+EL++N   +  +R E E  +G E+ V ESHL HL+YL ACIKE+              
Sbjct: 156 MAELIKNKPILENLRSELEREIG-EDIVRESHLPHLHYLHACIKETQRLHPAVPLLVHEA 214

Query: 356 XXDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
              T + M+ TIPK C V+VNVWAIGRDP  W+DPLTF P+ F+++S+ +KGN F+F PF
Sbjct: 215 L-QTCETMDCTIPKGCHVLVNVWAIGRDPETWEDPLTFSPQRFIDSSMGYKGNDFKFIPF 273


>OAY59868.1 hypothetical protein MANES_01G066500 [Manihot esculenta]
          Length = 498

 Score =  170 bits (430), Expect = 2e-47
 Identities = 82/178 (46%), Positives = 110/178 (61%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W+  I ERR         + DFLD L+  G  +  I  L+ ++F A TD++++ IEW M+
Sbjct: 252 WEAIINERREMNMSNSSTQRDFLDALIKNGSSNDQINMLLQEIFTAGTDSSSSTIEWMMT 311

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           EL+RNPK M++V +E E     E  V +SHL HL +L AC KE+                
Sbjct: 312 ELIRNPKYMKKVEEEIERETNQET-VKDSHLLHLPFLQACFKETLRMHPPGPLLVPHRAV 370

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T +VMNYTIPK+ +VM+N WAIGRDP IW+DPL F PE FL +S+DFKGN FE+ PF
Sbjct: 371 ETCNVMNYTIPKNAQVMINFWAIGRDPKIWEDPLVFKPERFLNSSLDFKGNDFEYIPF 428


>CDP15158.1 unnamed protein product [Coffea canephora]
          Length = 496

 Score =  169 bits (427), Expect = 6e-47
 Identities = 85/179 (47%), Positives = 105/179 (58%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W E IRERR   Y     + DFLDVLL   + D  I  L  ++F A +DT+T+ +EW M+
Sbjct: 253 WQEIIRERRGKKYQDSTRQKDFLDVLLQNDFSDDQINYLHLELFAAGSDTSTSTVEWAMA 312

Query: 182 ELLRNPKAMRRVRDEFEMV-VGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           ELLRNPK +  +R E + +    EN + ES L  L YL AC+KE                
Sbjct: 313 ELLRNPKFLDMIRQELDQIEYSVENVIKESDLTRLPYLQACVKEILRLHPPAPLLLPRRA 372

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  VMNYTIP    V+VNVWAIGRDP IW+DP +F PE FL   +DFKGN FEF PF
Sbjct: 373 TETCQVMNYTIPMGTEVLVNVWAIGRDPNIWEDPSSFNPERFLSCDLDFKGNDFEFLPF 431


>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
           3'-hydroxylase isozyme, putative [Ricinus communis]
          Length = 496

 Score =  168 bits (426), Expect = 9e-47
 Identities = 81/178 (45%), Positives = 116/178 (65%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           WD +I+ERR         K+DFLDV LS G++D  I  L+ ++F A  DTTTT +EW M+
Sbjct: 256 WDIYIKERRERHVKDA-RKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWAMA 314

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           ELL+    + +VR+E E  +G +  + ESH+  L YL+AC+KE+                
Sbjct: 315 ELLKEITVLEKVREELETEIGKDM-IRESHIPQLKYLNACVKETLRLHPPVPFLIPRRAP 373

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +  +VMNYTIPK  +++VNVWAIGRDP  W+DPL++ PE FL++++DFKG++FEF PF
Sbjct: 374 EACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPF 431


>XP_015896286.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Ziziphus jujuba]
          Length = 509

 Score =  168 bits (425), Expect = 2e-46
 Identities = 79/179 (44%), Positives = 114/179 (63%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGG-DAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W E I+ER+  G  G   ++ DFLD L+  G  +  +  L  ++FIA T T++++IEWT+
Sbjct: 262 WGEIIKERKEMGKTGNASSQKDFLDALIDTGCPEEQVNILFLELFIAGTGTSSSVIEWTL 321

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +EL+RNP+ ++ V DE   V+  E+ + E H ++L YL ACIKE+               
Sbjct: 322 AELIRNPRCLKIVEDELSRVI-YEDFITEIHTSNLTYLQACIKEAMRLYPTSPFLLPHRA 380

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            ++  VMNYTIPKD  ++VN+WAIGRDP  W+DPL F PE F+ +S+DFKGN F+F PF
Sbjct: 381 VESCQVMNYTIPKDSMILVNIWAIGRDPNYWEDPLVFKPERFINSSLDFKGNDFQFIPF 439


>XP_015878021.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Ziziphus jujuba]
          Length = 508

 Score =  167 bits (424), Expect = 2e-46
 Identities = 76/178 (42%), Positives = 113/178 (63%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W+  ++ER+        ++ DFLD ++   + D  I  L+ ++F A TDTTT  +EW M+
Sbjct: 266 WENIVKERKENRGKNSTSQRDFLDAMIENEFADHQINQLVMELFSAGTDTTTITVEWAMA 325

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           ELL+N +AM RV +E   V G +N ++ES+L+ L YL+AC KE+                
Sbjct: 326 ELLKNREAMERVLEELRSVFGDQNSISESNLSQLPYLEACFKETLRLHAPVPFLLPHCAP 385

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T +VMNYTIPK+ R++VNVWAIGRD   W+DPL F PE F+ + +DFKG+++E+ PF
Sbjct: 386 ETCEVMNYTIPKNARILVNVWAIGRDSAFWEDPLLFKPERFIGSELDFKGHNYEYLPF 443


>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  167 bits (422), Expect = 3e-46
 Identities = 84/178 (47%), Positives = 113/178 (63%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W   ++ERR  G  G ++K+DF+DVLL+AG +D  I AL+ ++F A T+T+ + IEW ++
Sbjct: 246 WGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAIT 303

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           EL + P  + ++R E   VVG +N V ES L HL YL A +KE+                
Sbjct: 304 ELTKKPLVVSKIRLELVNVVG-DNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRAL 362

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T  VMNYTIPK+C++MVN WAIGRDP  W DPL F PE FL + VD+KGN FE  PF
Sbjct: 363 ETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPF 420


>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1 AAC39452.1
           (S)-N-methylcoclaurine 3'-hydroxylase, partial
           [Eschscholzia californica]
          Length = 487

 Score =  166 bits (421), Expect = 4e-46
 Identities = 82/179 (45%), Positives = 111/179 (62%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAG-YDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W   ++ER+ A   DG  +K+DF+DV L +G  D  I AL+ ++F A T+T+ + IEW M
Sbjct: 245 WGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 304

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +EL +NPK   ++R E + VVG E  V ES   +L YL+A +KE+               
Sbjct: 305 TELTKNPKITAKIRSEIQTVVG-ERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 363

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  ++NYTIPKDC++MVN W IGRDP  W DPLTF PE FL +SVDF+GN F   PF
Sbjct: 364 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPF 422


>I3V6B1.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
           AltName: Full=Cytochrome P450 80BX AFK73713.1 cytochrome
           P450 [Papaver somniferum]
          Length = 440

 Score =  165 bits (417), Expect = 6e-46
 Identities = 82/179 (45%), Positives = 110/179 (61%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAGYD-GGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W   ++ERR A     GD K+DF+DVLL +G +D  I +L+ DMF A T+TT + IEW +
Sbjct: 246 WGAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWAL 305

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +EL +NP+    +R E   VVG +  V ES + +L YL A +KE+               
Sbjct: 306 TELTKNPQVTADIRSELLSVVG-KRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRA 364

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  V+NYTIPK+C++MVN W IGRDP  W DPL F P+ FL +S+DFKGN FE  PF
Sbjct: 365 LETCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPF 423


>XP_010112567.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 [Morus notabilis]
           EXC34114.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme
           1 [Morus notabilis]
          Length = 515

 Score =  166 bits (420), Expect = 9e-46
 Identities = 80/183 (43%), Positives = 113/183 (61%), Gaps = 5/183 (2%)
 Frame = +2

Query: 2   WDEFIRER---RAAGYDGGDAKS--DFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLI 166
           WD+ ++ER   R + Y     +   DFLD +L  GY D  I  L+ ++F A TD+++T +
Sbjct: 268 WDDIVKERKKMRKSYYKDASPRPQLDFLDAMLENGYPDFQINILLLELFSAGTDSSSTTV 327

Query: 167 EWTMSELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXX 346
           EW M+EL+RNPK M+ +R+E E  +G++  V E  L  L YL+ C+KE            
Sbjct: 328 EWLMAELVRNPKCMKILREELEREIGSQEMVMECDLPKLKYLECCLKEPLRLPPPAPLPI 387

Query: 347 XXXXXDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEF 526
                +T  VMNYT+PK+  ++VNVWAIGRDP  W+DPL F PE FL +S+DFKG +FE+
Sbjct: 388 PHRAAETCQVMNYTVPKNSEILVNVWAIGRDPNHWEDPLEFKPERFLNSSLDFKGTNFEY 447

Query: 527 TPF 535
            PF
Sbjct: 448 LPF 450


>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2 AAC39453.1
           (S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
           californica]
          Length = 488

 Score =  165 bits (418), Expect = 1e-45
 Identities = 82/179 (45%), Positives = 111/179 (62%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAG-YDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W   ++ER+ A   DG  +K+DF+DV L +G  D  I AL+ ++F A T+T+ + IEW M
Sbjct: 246 WGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 305

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +EL +NPK   ++R E + VVG E  V ES   +L YL+A +KE+               
Sbjct: 306 TELTKNPKITAKLRSELQTVVG-ERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 364

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  ++NYTIPKDC++MVN W IGRDP  W DPLTF PE FL +SVDF+GN F   PF
Sbjct: 365 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPF 423


>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
           californica [Expression vector pWCD2353]
          Length = 490

 Score =  165 bits (418), Expect = 1e-45
 Identities = 82/179 (45%), Positives = 111/179 (62%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAG-YDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W   ++ER+ A   DG  +K+DF+DV L +G  D  I AL+ ++F A T+T+ + IEW M
Sbjct: 246 WGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAM 305

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +EL +NPK   ++R E + VVG E  V ES   +L YL+A +KE+               
Sbjct: 306 TELTKNPKITAKLRSELQTVVG-ERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 364

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  ++NYTIPKDC++MVN W IGRDP  W DPLTF PE FL +SVDF+GN F   PF
Sbjct: 365 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPF 423


>XP_002274586.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  165 bits (418), Expect = 1e-45
 Identities = 77/178 (43%), Positives = 113/178 (63%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKSDFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTMS 181
           W++ ++ERR     G  +  DFLD LL  G+ED  I  +I ++F A  +T++  +EW M+
Sbjct: 257 WEDILKERRKQRIHGS-SHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMA 315

Query: 182 ELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXXX 361
           EL++N  AM ++ +E   ++G E+ V ESHL HL YL AC+KE+                
Sbjct: 316 ELIKNQDAMHKLCNELTQIIG-ESPVRESHLPHLPYLQACVKETLRLHPTGPLLLPHRAT 374

Query: 362 DTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
           +T  +M YTIPKD  + VN+WA+GRDPG W+DPL+F PE FL++ ++FKGN FE+ PF
Sbjct: 375 ETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFLDSKLEFKGNDFEYIPF 432


>XP_009610421.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
           [Nicotiana tomentosiformis]
          Length = 489

 Score =  164 bits (415), Expect = 3e-45
 Identities = 78/179 (43%), Positives = 114/179 (63%), Gaps = 1/179 (0%)
 Frame = +2

Query: 2   WDEFIRERRAAGYDGGDAKS-DFLDVLLSAGYEDLPIKALISDMFIAATDTTTTLIEWTM 178
           W E ++E+R A   G   K+ DFLD+++ +G+ DL I  ++ ++  A TDTTT+ IEW M
Sbjct: 249 WAEVVKEKRQAISCGSSNKNQDFLDIMIDSGFSDLQINIILMELIAAGTDTTTSTIEWAM 308

Query: 179 SELLRNPKAMRRVRDEFEMVVGAENGVNESHLNHLNYLDACIKESXXXXXXXXXXXXXXX 358
           +ELLRN K M +++ E    +G+   + ES+++ L YL AC+KE+               
Sbjct: 309 AELLRNKKVMHKLQAEIRSKIGSNANIRESNISELPYLAACVKETLRIHPPTPFLVPRRA 368

Query: 359 XDTGDVMNYTIPKDCRVMVNVWAIGRDPGIWKDPLTFLPETFLETSVDFKGNHFEFTPF 535
            +T  +MN+TIPK+ R+ VNVWAIGRD   W+D L+F PE FL++SVDF+G  FEF PF
Sbjct: 369 PETCKLMNFTIPKNSRLFVNVWAIGRDSKTWEDALSFRPERFLDSSVDFRGQDFEFIPF 427