BLASTX nr result
ID: Magnolia22_contig00010863
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Magnolia22_contig00010863
(612 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BAB71802.1 coclaurine N-methyltransferase [Coptis japonica] 307 e-102
C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNM... 295 5e-97
5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4... 296 6e-97
Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short... 293 2e-96
AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexan... 291 1e-95
5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Compl... 292 2e-95
ACO90253.1 putative N-methyltransferase [Thalictrum flavum] 286 9e-94
ANY58196.1 putative N-methyltransferase [synthetic construct] 270 3e-87
ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata] 266 8e-86
Q7XB08.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short... 262 3e-84
AKH61489.1 N-methyltransferase [Expression vector pWCD2353] 262 3e-84
XP_006843159.1 PREDICTED: (S)-coclaurine N-methyltransferase [Am... 260 2e-83
ANY58195.1 TNMT [synthetic construct] 257 2e-82
C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltran... 257 2e-82
ANY58202.1 putative N-methyltransferase [synthetic construct] 255 6e-82
ANY58201.1 putative N-methyltransferase [synthetic construct] 255 2e-81
XP_008393607.1 PREDICTED: (S)-coclaurine N-methyltransferase-lik... 255 2e-81
XP_010261992.1 PREDICTED: (S)-coclaurine N-methyltransferase-lik... 255 2e-81
ONI33134.1 hypothetical protein PRUPE_1G407700 [Prunus persica] 252 2e-81
XP_004303486.1 PREDICTED: (S)-coclaurine N-methyltransferase [Fr... 254 4e-81
>BAB71802.1 coclaurine N-methyltransferase [Coptis japonica]
Length = 358
Score = 307 bits (787), Expect = e-102
Identities = 143/204 (70%), Positives = 172/204 (84%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYKPTYEEQ+A++ +L SLR+M I TE +TLDSQ+YE+PI FLK+M G +KGSC
Sbjct: 41 RLQWGYKPTYEEQIAEIQNLTHSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSC 100
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF ++STTLDEAEIAMLDLYCERAQI+DGQSVLDLGCGQGA+TLHVAQKYKNC VT +T
Sbjct: 101 CYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVT 160
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK +IEE+ + L NVE+ LAD++ EM T+DRI+VI L EHMKNYEL LRK+S
Sbjct: 161 NSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKIS 220
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+W++ +GLLFLEH CHK FAY YE
Sbjct: 221 EWISKDGLLFLEHICHKTFAYHYE 244
>C3SBW0.1 RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT ACO90251.1
pavine N-methyltransferase [Thalictrum flavum]
ANY58204.1 PNMT [synthetic construct]
Length = 356
Score = 295 bits (754), Expect = 5e-97
Identities = 138/204 (67%), Positives = 169/204 (82%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RLKWGYKPT+E+QLAQ++ A+SL+ M + E DTLD+++YE+P+ FL +M GKT+K S
Sbjct: 39 RLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSP 98
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
YF DESTTLDE+E+ M+DLYCERAQI+DGQS+LDLGCG G++TLHVAQKY+ C VTGIT
Sbjct: 99 GYFKDESTTLDESEVYMMDLYCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGIT 158
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK FI +QCK L LSNVEIIL DV+K E E T+DRI + L+EHMKNYELFL+KVS
Sbjct: 159 NSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVS 218
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
W+ GLLF+EH CHK+FAYQYE
Sbjct: 219 TWIAQYGLLFVEHHCHKVFAYQYE 242
>5KN4_A Chain A, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KN4_B Chain
B, Pavine N-methyltransferase Apoenzyme Ph 6.0 5KOC_A
Chain A, Pavine N-methyltransferase In Complex With
S-adenosylmethionine Ph 7 5KOC_B Chain B, Pavine
N-methyltransferase In Complex With S-adenosylmethionine
Ph 7 5KOK_A Chain A, Pavine N-methyltransferase In
Complex With Tetrahydropapaverine And S-
Adenosylhomocysteine Ph 7.25 5KOK_B Chain B, Pavine
N-methyltransferase In Complex With Tetrahydropapaverine
And S- Adenosylhomocysteine Ph 7.25 5KPG_A Chain A,
Pavine N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7 5KPG_B Chain B, Pavine
N-methyltransferase In Complex With
S-adenosylhomocysteine Ph 7
Length = 397
Score = 296 bits (757), Expect = 6e-97
Identities = 138/204 (67%), Positives = 170/204 (83%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RLKWGYKPT+E+QLAQ++ A+SL+ M + E DTLD+++YE+P+ FL +M GKT+K S
Sbjct: 80 RLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSP 139
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
YF DESTTLDE+E+ M+DLYCERAQI+DGQS+LDLGCG G++TLHVAQKY+ C VTGIT
Sbjct: 140 GYFKDESTTLDESEVYMMDLYCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGIT 199
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK FI +QCK L LSNVEIIL DV+K E E T+DRI + L+EHMKNYELFL+KVS
Sbjct: 200 NSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVS 259
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
W+ +GLLF+EH CHK+FAYQYE
Sbjct: 260 TWIAQDGLLFVEHHCHKVFAYQYE 283
>Q5C9L6.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
AAU20766.1 (S)-coclaurine N-methyltransferase
[Thalictrum flavum subsp. glaucum] ANY58203.1 CNMT
[synthetic construct]
Length = 361
Score = 293 bits (751), Expect = 2e-96
Identities = 138/204 (67%), Positives = 168/204 (82%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WG K TYEEQ+AQ+++L SLR+M I TE +TLD QMYEVPI FLK+M G +KGSC
Sbjct: 43 RLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSC 102
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF ++STTLDEAEIAML+LYCERAQI+DG SVLDLGCGQGA+TL+VAQKYKN VT +T
Sbjct: 103 CYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVT 162
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK FIEE+ + LSNVE++LAD++ +M T+DRI+V+ L EHMKNYEL LRK+
Sbjct: 163 NSVSQKEFIEEESRKRNLSNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIK 222
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GLLF+EH CHK FAY YE
Sbjct: 223 EWMAKDGLLFVEHICHKTFAYHYE 246
>AJD20224.1 coclaurine N-methyltransferase [Sinopodophyllum hexandrum]
Length = 370
Score = 291 bits (746), Expect = 1e-95
Identities = 134/204 (65%), Positives = 169/204 (82%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYK T+++QLAQ++D A SLR+MNI E DTLDS +YEVPISFL++M G +KGS
Sbjct: 53 RLRWGYKSTHQQQLAQLVDFAHSLRRMNIADEVDTLDSNIYEVPISFLRIMNGPMLKGSG 112
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF +STTL EAEIAMLDLYCERAQ+ DG ++LDLGCGQGA+TLH+A+KY NC VT IT
Sbjct: 113 CYFPHDSTTLAEAEIAMLDLYCERAQVRDGHTILDLGCGQGALTLHIAKKYTNCRVTAIT 172
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQKHFI++QC L+NVE++LAD++K + +FDR+ VI LLEHMKNYEL L+K+S
Sbjct: 173 NSVSQKHFIQDQCIKSNLTNVEVLLADITKHDTSHSFDRVFVIELLEHMKNYELLLKKIS 232
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM+ +GLLF+EH CHK FAY Y+
Sbjct: 233 KWMSQDGLLFIEHICHKTFAYHYD 256
>5KPC_A Chain A, Pavine N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6 5KPC_B Chain B, Pavine
N-methyltransferase H206a Mutant In Complex With S-
Adenosylmethionine Ph 6
Length = 397
Score = 292 bits (747), Expect = 2e-95
Identities = 137/204 (67%), Positives = 169/204 (82%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RLKWGYKPT+E+QLAQ++ A+SL+ M + E DTLD+++YE+P+ FL +M GKT+K S
Sbjct: 80 RLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSP 139
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
YF DESTTLDE+E+ M+DLYCERAQI+DGQS+LDLGCG G++TLHVAQKY+ C VTGIT
Sbjct: 140 GYFKDESTTLDESEVYMMDLYCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGIT 199
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK FI +QCK L LSNVEIIL DV+K E E T+DRI + L+E MKNYELFL+KVS
Sbjct: 200 NSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDRIFAVALIEAMKNYELFLKKVS 259
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
W+ +GLLF+EH CHK+FAYQYE
Sbjct: 260 TWIAQDGLLFVEHHCHKVFAYQYE 283
>ACO90253.1 putative N-methyltransferase [Thalictrum flavum]
Length = 351
Score = 286 bits (732), Expect = 9e-94
Identities = 134/204 (65%), Positives = 164/204 (80%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYK TYEEQ+AQVLDL SLR MNI E DTLDS MYEVP+ FLK+M G +K S
Sbjct: 34 RLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPLDFLKIMNGSALKMSS 93
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF D+STTLDEAEIAM+DL+CERAQI+DG SVLDLGCG G++T +VAQKYK HVT +T
Sbjct: 94 CYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTFYVAQKYKKSHVTAVT 153
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SVSQK +IEE+ + LSNVE++LAD++ EM T+DRI+V+GL EHMKNY L L+K+S
Sbjct: 154 NSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGLFEHMKNYGLLLKKIS 213
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GLLF++H CHK FA+ E
Sbjct: 214 EWMAKDGLLFVQHVCHKTFAFHCE 237
>ANY58196.1 putative N-methyltransferase [synthetic construct]
Length = 357
Score = 270 bits (689), Expect = 3e-87
Identities = 121/204 (59%), Positives = 167/204 (81%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RLKWGYK T+ +QL LD KSL++M++ + + ++S+ YE+P++FL+ MFGKT+K SC
Sbjct: 40 RLKWGYKSTHVQQLEFNLDFVKSLKQMDMSGKMEVINSESYELPVAFLRAMFGKTLKQSC 99
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF DES TLDEAE A +LYCERAQI+DGQ VLD+GCGQG++ +H+AQKYKNCHVTGIT
Sbjct: 100 CYFEDESMTLDEAETAAHELYCERAQIKDGQRVLDIGCGQGSLIIHIAQKYKNCHVTGIT 159
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK+ IEE+C+NL+LSNVE+ILADV++ + + TFDRI++I +EHMKN +LFL+K+S
Sbjct: 160 NSNAQKNIIEEECRNLKLSNVEVILADVTQYDTKDTFDRIILIEAVEHMKNIDLFLKKIS 219
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GL+F++ CHK F + +E
Sbjct: 220 KWMKDDGLMFIDCICHKTFGHHFE 243
>ADP76529.1 coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 266 bits (680), Expect = 8e-86
Identities = 129/208 (62%), Positives = 166/208 (79%), Gaps = 4/208 (1%)
Frame = -1
Query: 612 RLKW--GYKPTYEEQLAQVLDLAKSLRKMNI-GTETDTLDSQMYEVPISFLKLMFGKTVK 442
RL+W KPTYEEQ A+++ L K+LR+M I G ++D L+S +Y++P+SFLK+ FGK +K
Sbjct: 36 RLRWYCQKKPTYEEQTAEIVALVKALRQMGITGDQSDQLNSDLYDMPMSFLKITFGKLLK 95
Query: 441 GSCCYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVT 262
S YF D+S TLDEAE AMLDLYCERAQ++DGQ +LDLGCGQGA TLH AQKYK HVT
Sbjct: 96 ESGSYFKDDSMTLDEAEEAMLDLYCERAQLKDGQKILDLGCGQGAFTLHAAQKYKKSHVT 155
Query: 261 GITYSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLR 82
+T S +QK +IE+QC+ L+LSNVE++L D+++L MEATFDRI+VIGLLEHMKNY L L+
Sbjct: 156 AVTNSATQKKYIEDQCQILELSNVEVLLEDITQLTMEATFDRIIVIGLLEHMKNYGLLLQ 215
Query: 81 KVSQWMTP-NGLLFLEHACHKMFAYQYE 1
+SQWM P + LLF++H CHK + YQ E
Sbjct: 216 NISQWMAPADSLLFIDHVCHKSYFYQCE 243
>Q7XB08.1 RecName: Full=(S)-coclaurine N-methyltransferase; Short=PsCNMT
AAP45316.1 S-adenosyl-L-methionine:coclaurine
N-methyltransferase [Papaver somniferum] AHF27396.1
S-adenosyl-L-methionine:coclaurine N-methyltransferase
[synthetic construct]
Length = 351
Score = 262 bits (669), Expect = 3e-84
Identities = 123/204 (60%), Positives = 156/204 (76%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYK T+EEQL+Q+LDL SL+ M + TE + LD ++YE P+ FLK+ G +K S
Sbjct: 34 RLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPMEFLKIQHGSNMKQSA 93
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
Y+ DESTTLDEAEIAMLDLY ERAQI+DGQSVLDLGCG GAV L A K+K C TG+T
Sbjct: 94 GYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVALFGANKFKKCQFTGVT 153
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SV QK +IE +CK L+L+NV+++LAD++ E E FDRI + L+EHMKNY+L L+K+S
Sbjct: 154 SSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVELIEHMKNYQLLLKKIS 213
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GLLF+EH CHK AY YE
Sbjct: 214 EWMKDDGLLFVEHVCHKTLAYHYE 237
>AKH61489.1 N-methyltransferase [Expression vector pWCD2353]
Length = 353
Score = 262 bits (669), Expect = 3e-84
Identities = 123/204 (60%), Positives = 156/204 (76%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYK T+EEQL+Q+LDL SL+ M + TE + LD ++YE P+ FLK+ G +K S
Sbjct: 34 RLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPMEFLKIQHGSNMKQSA 93
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
Y+ DESTTLDEAEIAMLDLY ERAQI+DGQSVLDLGCG GAV L A K+K C TG+T
Sbjct: 94 GYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVALFGANKFKKCQFTGVT 153
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
SV QK +IE +CK L+L+NV+++LAD++ E E FDRI + L+EHMKNY+L L+K+S
Sbjct: 154 SSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVELIEHMKNYQLLLKKIS 213
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GLLF+EH CHK AY YE
Sbjct: 214 EWMKDDGLLFVEHVCHKTLAYHYE 237
>XP_006843159.1 PREDICTED: (S)-coclaurine N-methyltransferase [Amborella
trichopoda] ERN04834.1 hypothetical protein
AMTR_s00146p00043700 [Amborella trichopoda]
Length = 356
Score = 260 bits (664), Expect = 2e-83
Identities = 122/204 (59%), Positives = 155/204 (75%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+ GYKPT E QL+Q+L SL++M I E Q YE+P SF KL+ GK +K SC
Sbjct: 42 RLRSGYKPTSEAQLSQLLQFIHSLKEMPIAVEMGKAKDQHYELPTSFFKLVLGKNLKYSC 101
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF ++ TL+EAE AML+LYCERAQI+DG SVLD+GCG G+++L++AQKY NCHVTGI
Sbjct: 102 CYFKNKYITLEEAETAMLELYCERAQIKDGHSVLDVGCGWGSLSLYIAQKYSNCHVTGIC 161
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK FIEEQC+ +LSNVEII D+S EM+ATFDRI+ I + EHMKNY+ LRK+S
Sbjct: 162 NSTTQKEFIEEQCRERKLSNVEIIATDISNFEMDATFDRIMSIEMFEHMKNYQALLRKIS 221
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM P+GLLF+ + CHK FAY +E
Sbjct: 222 KWMKPDGLLFIHYFCHKTFAYHFE 245
>ANY58195.1 TNMT [synthetic construct]
Length = 350
Score = 257 bits (657), Expect = 2e-82
Identities = 113/204 (55%), Positives = 164/204 (80%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
R++WGYKPT+E+QLA LD K L++M + E DT++ + YE+P +FL+ +FGKTVK SC
Sbjct: 33 RIQWGYKPTHEKQLAFNLDFIKGLKEMVMSGEIDTMNKETYELPTAFLEAVFGKTVKQSC 92
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF DE++T+DEAE A +LYCERAQI+DGQ+VLD+GCGQG + L++A+KYKNCHVTG+T
Sbjct: 93 CYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLT 152
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +Q ++IE+Q + L+L+NV++I ADV+K + + T+DRI+V+ +EHMKN +LF++K+S
Sbjct: 153 NSKAQANYIEQQAEKLELANVDVIFADVTKFDTDKTYDRILVVETIEHMKNIQLFMKKLS 212
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
WMT + LLF++H HK F + +E
Sbjct: 213 TWMTEDSLLFVDHISHKTFNHNFE 236
>C3SBS8.1 RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT ACO90222.1 tetrahydroprotoberberine
N-methyltransferase [Eschscholzia californica]
Length = 350
Score = 257 bits (657), Expect = 2e-82
Identities = 113/204 (55%), Positives = 164/204 (80%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
R++WGYKPT+E+QLA LD K L++M + E DT++ + YE+P +FL+ +FGKTVK SC
Sbjct: 33 RIEWGYKPTHEKQLAFNLDFIKGLKEMVMSGEIDTMNKETYELPTAFLEAVFGKTVKQSC 92
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF DE++T+DEAE A +LYCERAQI+DGQ+VLD+GCGQG + L++A+KYKNCHVTG+T
Sbjct: 93 CYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLT 152
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +Q ++IE+Q + L+L+NV++I ADV+K + + T+DRI+V+ +EHMKN +LF++K+S
Sbjct: 153 NSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMKNIQLFMKKLS 212
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
WMT + LLF++H HK F + +E
Sbjct: 213 TWMTEDSLLFVDHISHKTFNHNFE 236
>ANY58202.1 putative N-methyltransferase [synthetic construct]
Length = 333
Score = 255 bits (652), Expect = 6e-82
Identities = 118/204 (57%), Positives = 159/204 (77%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYKPT+E+QLA D KSL+ M++ E DT++ + YE+P +FL+ FGKT+K S
Sbjct: 43 RLQWGYKPTHEQQLAFNFDFIKSLKHMDMSGEIDTMNEETYELPTAFLEAAFGKTIKQSG 102
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF DE+TTLDEAE A +LYCERAQI+DGQSVLD+GCGQG + LH+AQKYKNCHVTG+T
Sbjct: 103 CYFKDETTTLDEAEEASHELYCERAQIKDGQSVLDIGCGQGGLVLHIAQKYKNCHVTGLT 162
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +Q ++I Q + LQLSNV++I ADV+K + + T+DRI+VI +EHMKN +LF++K+S
Sbjct: 163 NSKAQMNYILMQAEKLQLSNVDVISADVTKFDTDKTYDRILVIEAIEHMKNIQLFMKKLS 222
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
WM + LLF++H HK F + +E
Sbjct: 223 TWMKEDSLLFVDHISHKTFNHHFE 246
>ANY58201.1 putative N-methyltransferase [synthetic construct]
Length = 365
Score = 255 bits (652), Expect = 2e-81
Identities = 115/204 (56%), Positives = 162/204 (79%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGY+PT+ +QL+ LD +SL+KM++ E +T++ + YE+P +FL+ FGKT+K S
Sbjct: 48 RLQWGYQPTHHQQLSFNLDFIRSLKKMDMSGEIETMNEETYELPTAFLEAAFGKTIKQSA 107
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF D+STTLDEAE A +LYCERAQI+DGQ+VLD+GCGQG + LH+AQKYKNC VTGIT
Sbjct: 108 CYFKDKSTTLDEAEEASHELYCERAQIKDGQTVLDVGCGQGGLVLHIAQKYKNCRVTGIT 167
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +Q ++I Q + LQLSNV++ILADV+K + + T+DRI+VI +EHMKN +LF++K+S
Sbjct: 168 NSKAQMNYIVMQAEKLQLSNVDVILADVTKFDSDQTYDRILVIETIEHMKNIQLFMKKLS 227
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
W+ + LLF++H CHK F++ +E
Sbjct: 228 TWLKKDSLLFVDHICHKTFSHHFE 251
>XP_008393607.1 PREDICTED: (S)-coclaurine N-methyltransferase-like [Malus
domestica]
Length = 355
Score = 255 bits (651), Expect = 2e-81
Identities = 120/204 (58%), Positives = 157/204 (76%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+ GY+P+ E QL+ +L +SL++M I +TD +Q YEVP SF K++ GK +K SC
Sbjct: 41 RLRSGYRPSSELQLSDLLQFVQSLKEMPIAIKTDDPKAQHYEVPTSFFKMVLGKNLKYSC 100
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF DES+TL++AE AML+LYCER+QI DG +VLD+GCG G+++L++AQKY NC VTGI
Sbjct: 101 CYFNDESSTLEDAEKAMLELYCERSQIRDGHTVLDVGCGWGSLSLYIAQKYNNCKVTGIC 160
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK FIEEQC+NLQL NVEII+ D+S EMEA+FDRI I + EHMKNY+ L+K+S
Sbjct: 161 NSTTQKAFIEEQCRNLQLQNVEIIVGDISTFEMEASFDRIFSIEMFEHMKNYKDLLKKIS 220
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
WM +GLLF+ H CHK FAY +E
Sbjct: 221 GWMKDDGLLFVHHFCHKAFAYHFE 244
>XP_010261992.1 PREDICTED: (S)-coclaurine N-methyltransferase-like [Nelumbo
nucifera]
Length = 357
Score = 255 bits (651), Expect = 2e-81
Identities = 117/204 (57%), Positives = 160/204 (78%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+WGYKP+ + QL+ +L SL++M I +TD SQ YE+P SF KL+ GK +K SC
Sbjct: 41 RLRWGYKPSSQLQLSDLLQFVHSLKEMPIAIKTDLPKSQHYELPTSFFKLVLGKNLKYSC 100
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF+D+S+TL++AE AML+LYCERAQI+DGQSVLD+GCG G+++L++AQK+ +C +TGI
Sbjct: 101 CYFLDKSSTLEDAEKAMLELYCERAQIKDGQSVLDVGCGWGSLSLYIAQKFSSCRITGIC 160
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK +IEEQC+ L+L NVEII+AD+S EMEA+FDRI+ I + EHMKNY+ L K+S
Sbjct: 161 NSKTQKAYIEEQCRELKLQNVEIIVADISTFEMEASFDRILSIEMFEHMKNYKALLNKIS 220
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM + LLF+ + CHK FAY +E
Sbjct: 221 KWMKEDSLLFVNYFCHKAFAYHFE 244
>ONI33134.1 hypothetical protein PRUPE_1G407700 [Prunus persica]
Length = 271
Score = 252 bits (643), Expect = 2e-81
Identities = 120/204 (58%), Positives = 158/204 (77%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+ GYKPT E QL+ +L +SL++M I +TD +Q YEVP SF K++ GK +K SC
Sbjct: 41 RLRSGYKPTSELQLSDLLQFVQSLKEMPIAIKTDDPKAQHYEVPTSFFKIVLGKNLKYSC 100
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
YF D S+TL+EAE AML+LYCER+QI++G +VLD+GCG G+++L++AQKY NC VTGI
Sbjct: 101 YYFTDGSSTLEEAEKAMLELYCERSQIKNGHTVLDVGCGWGSLSLYIAQKYSNCKVTGIC 160
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK FIEEQC+NLQL NVEII+AD+S EMEA++DRI I + EHMKNY+ L+K+S
Sbjct: 161 NSTTQKAFIEEQCRNLQLRNVEIIVADISTFEMEASYDRIFSIEMFEHMKNYKDLLKKIS 220
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
+WM +GLLF+ H CHK FAY +E
Sbjct: 221 RWMKQDGLLFVHHFCHKAFAYHFE 244
>XP_004303486.1 PREDICTED: (S)-coclaurine N-methyltransferase [Fragaria vesca
subsp. vesca]
Length = 355
Score = 254 bits (649), Expect = 4e-81
Identities = 118/204 (57%), Positives = 159/204 (77%)
Frame = -1
Query: 612 RLKWGYKPTYEEQLAQVLDLAKSLRKMNIGTETDTLDSQMYEVPISFLKLMFGKTVKGSC 433
RL+ GY+P+ E QL+Q+L KSL++M I +TD +Q YEVP SF K++ G +K SC
Sbjct: 41 RLRTGYQPSSELQLSQLLQFVKSLKEMPIAIKTDDAKAQHYEVPTSFFKMVLGNNLKYSC 100
Query: 432 CYFMDESTTLDEAEIAMLDLYCERAQIEDGQSVLDLGCGQGAVTLHVAQKYKNCHVTGIT 253
CYF D+S++L++AE AML++YCER+QI+DG SVLD+GCG G+++L++A+KYKNC VTGI
Sbjct: 101 CYFTDKSSSLEDAEKAMLEMYCERSQIKDGDSVLDVGCGWGSLSLYIAEKYKNCKVTGIC 160
Query: 252 YSVSQKHFIEEQCKNLQLSNVEIILADVSKLEMEATFDRIVVIGLLEHMKNYELFLRKVS 73
S +QK FIEEQC+N QL NVEII+AD+S EMEA++DRI I + EHMKNY+ L+K+S
Sbjct: 161 NSATQKAFIEEQCRNRQLENVEIIVADISTFEMEASYDRIFSIEMFEHMKNYKDLLKKIS 220
Query: 72 QWMTPNGLLFLEHACHKMFAYQYE 1
WM +GLLF+ H CHK FAY +E
Sbjct: 221 GWMKQDGLLFVHHFCHKAFAYHFE 244