BLASTX nr result
ID: Lithospermum23_contig00025691
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00025691
(350 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KYP70965.1 Retrovirus-related Pol polyprotein from transposon TN... 165 9e-49
KYP64905.1 Retrovirus-related Pol polyprotein from transposon TN... 173 1e-48
XP_010669529.1 PREDICTED: uncharacterized protein LOC104886740 [... 175 1e-48
XP_017415205.1 PREDICTED: retrovirus-related Pol polyprotein fro... 174 3e-48
XP_017415203.1 PREDICTED: retrovirus-related Pol polyprotein fro... 174 3e-48
XP_017415202.1 PREDICTED: retrovirus-related Pol polyprotein fro... 174 3e-48
KYP61781.1 Retrovirus-related Pol polyprotein from transposon TN... 172 2e-47
GAU11040.1 hypothetical protein TSUD_113330 [Trifolium subterran... 170 7e-47
KYP34077.1 Retrovirus-related Pol polyprotein from transposon TN... 165 9e-47
KYP57917.1 Retrovirus-related Pol polyprotein from transposon TN... 164 3e-46
GAU21017.1 hypothetical protein TSUD_201660 [Trifolium subterran... 168 3e-46
KYP56739.1 Retrovirus-related Pol polyprotein from transposon TN... 164 8e-46
GAU47808.1 hypothetical protein TSUD_404130 [Trifolium subterran... 166 2e-45
KYP66022.1 Retrovirus-related Pol polyprotein from transposon TN... 164 2e-45
GAU34891.1 hypothetical protein TSUD_144220 [Trifolium subterran... 166 3e-45
CAN73272.1 hypothetical protein VITISV_013115 [Vitis vinifera] 165 4e-45
KYP63761.1 Retrovirus-related Pol polyprotein from transposon TN... 164 7e-45
KYP43009.1 Retrovirus-related Pol polyprotein from transposon TN... 164 7e-45
KYP77073.1 Retrovirus-related Pol polyprotein from transposon TN... 164 7e-45
XP_010667912.1 PREDICTED: uncharacterized protein LOC104884908 [... 164 7e-45
>KYP70965.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 263
Score = 165 bits (417), Expect = 9e-49
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 8 LETNGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 67
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 68 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 115
>KYP64905.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 651
Score = 173 bits (439), Expect = 1e-48
Identities = 80/116 (68%), Positives = 95/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE+NGTW+L L GKKA+G +WVY+IKYNSDGS+ERFK RLV+ GN+QVE +DYN+TFA
Sbjct: 412 LENNGTWTLEPLPPGKKAIGCKWVYRIKYNSDGSIERFKARLVILGNNQVEGLDYNETFA 471
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVTVR LAVA + WELH+MDVHNAFL GDL EEVYM++PPGF+ G V
Sbjct: 472 PVAKMVTVRTLLAVAAARKWELHQMDVHNAFLHGDLEEEVYMKLPPGFRSQASGKV 527
>XP_010669529.1 PREDICTED: uncharacterized protein LOC104886740 [Beta vulgaris
subsp. vulgaris]
Length = 863
Score = 175 bits (443), Expect = 1e-48
Identities = 82/116 (70%), Positives = 94/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTW + L GKKALG++WVYKIKYNSDGS+ERFK RLVVFGNHQ E +DY +TFA
Sbjct: 593 LEKNGTWIMEPLPAGKKALGSKWVYKIKYNSDGSIERFKARLVVFGNHQTEGLDYTETFA 652
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMV VRAFLA+ K WELH+MDVHNAFL G+LSEEVYM++PPGFQ +V
Sbjct: 653 PVAKMVIVRAFLALVASKKWELHQMDVHNAFLHGELSEEVYMKLPPGFQSPSSNMV 708
>XP_017415205.1 PREDICTED: retrovirus-related Pol polyprotein from transposon TNT
1-94 isoform X3 [Vigna angularis]
Length = 982
Score = 174 bits (441), Expect = 3e-48
Identities = 80/116 (68%), Positives = 95/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
L++NGTW ++ L GKK LG +W+YKIKY+SDGS+ERFK RLVV GNHQVE IDY++TFA
Sbjct: 496 LQNNGTWDIIALPAGKKPLGCKWIYKIKYHSDGSIERFKARLVVLGNHQVEGIDYDETFA 555
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVT+R LAVAV K WELH+MDVHNAFL GDL E VYM++PPGF + GLV
Sbjct: 556 PVAKMVTIRTILAVAVAKDWELHQMDVHNAFLHGDLDEAVYMKLPPGFLVSQNGLV 611
>XP_017415203.1 PREDICTED: retrovirus-related Pol polyprotein from transposon TNT
1-94 isoform X2 [Vigna angularis]
Length = 1435
Score = 174 bits (441), Expect = 3e-48
Identities = 80/116 (68%), Positives = 95/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
L++NGTW ++ L GKK LG +W+YKIKY+SDGS+ERFK RLVV GNHQVE IDY++TFA
Sbjct: 949 LQNNGTWDIIALPAGKKPLGCKWIYKIKYHSDGSIERFKARLVVLGNHQVEGIDYDETFA 1008
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVT+R LAVAV K WELH+MDVHNAFL GDL E VYM++PPGF + GLV
Sbjct: 1009 PVAKMVTIRTILAVAVAKDWELHQMDVHNAFLHGDLDEAVYMKLPPGFLVSQNGLV 1064
>XP_017415202.1 PREDICTED: retrovirus-related Pol polyprotein from transposon TNT
1-94 isoform X1 [Vigna angularis]
Length = 1472
Score = 174 bits (441), Expect = 3e-48
Identities = 80/116 (68%), Positives = 95/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
L++NGTW ++ L GKK LG +W+YKIKY+SDGS+ERFK RLVV GNHQVE IDY++TFA
Sbjct: 986 LQNNGTWDIIALPAGKKPLGCKWIYKIKYHSDGSIERFKARLVVLGNHQVEGIDYDETFA 1045
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVT+R LAVAV K WELH+MDVHNAFL GDL E VYM++PPGF + GLV
Sbjct: 1046 PVAKMVTIRTILAVAVAKDWELHQMDVHNAFLHGDLDEAVYMKLPPGFLVSQNGLV 1101
>KYP61781.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94, partial
[Cajanus cajan]
Length = 1413
Score = 172 bits (436), Expect = 2e-47
Identities = 79/116 (68%), Positives = 95/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE+NGTW+L L GKKA+G +WVY+IKYNSDGS+ERFK RLV+ GN+QVE +DYN+TFA
Sbjct: 927 LENNGTWTLEPLPPGKKAIGCKWVYRIKYNSDGSIERFKARLVILGNNQVEGLDYNETFA 986
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVT+R LAVA + WELH+MDVHNAFL GDL EEVYM++PPGF+ G V
Sbjct: 987 PVAKMVTMRTLLAVAAARKWELHQMDVHNAFLHGDLKEEVYMKLPPGFRSQASGKV 1042
>GAU11040.1 hypothetical protein TSUD_113330 [Trifolium subterraneum]
Length = 1355
Score = 170 bits (431), Expect = 7e-47
Identities = 81/116 (69%), Positives = 94/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
L++NGTW L L GKKA+G +WVYKIKY+SDGSVER+K RLV+ GN+Q E +DYN+TFA
Sbjct: 869 LDNNGTWILETLPPGKKAIGCKWVYKIKYHSDGSVERYKARLVILGNNQTEGLDYNETFA 928
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVTVR FLAVAV K WELH+MDVHNAFL GDL+EEVYM+ PPGF G V
Sbjct: 929 PVAKMVTVRVFLAVAVAKQWELHQMDVHNAFLHGDLAEEVYMKPPPGFHPPTSGHV 984
>KYP34077.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 459
Score = 165 bits (417), Expect = 9e-47
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 254 LETNGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 313
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 314 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 361
>KYP57917.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 494
Score = 164 bits (415), Expect = 3e-46
Identities = 76/108 (70%), Positives = 89/108 (82%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE N TWS+ L GKKALG +WVY+IKYNSDG++ERFK RLVV GNHQ +DI+Y +TFA
Sbjct: 8 LERNNTWSMQPLPSGKKALGCKWVYRIKYNSDGTIERFKARLVVLGNHQEKDINYTETFA 67
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA KSWELH+MDVHNAFL GDL +EV++R+PPGF
Sbjct: 68 PVVKMVTVRTVLAVAAAKSWELHQMDVHNAFLHGDLHDEVFIRMPPGF 115
>GAU21017.1 hypothetical protein TSUD_201660 [Trifolium subterraneum]
Length = 1252
Score = 168 bits (426), Expect = 3e-46
Identities = 74/108 (68%), Positives = 93/108 (86%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE+N TW++ L GKK++G +WVYKIKY+SDGS+ER+K RLV+ GN+QVE +DYN+TFA
Sbjct: 766 LENNNTWTIETLPHGKKSIGCKWVYKIKYHSDGSIERYKARLVILGNNQVEGLDYNETFA 825
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
VAKMVTVR FLAVA ++WELH+MDVHNAFL GDL EE+YM++PPGF
Sbjct: 826 PVAKMVTVRTFLAVAAARNWELHQMDVHNAFLHGDLEEEIYMKLPPGF 873
>KYP56739.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 581
Score = 164 bits (416), Expect = 8e-46
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 95 LEANGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 154
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 155 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 202
>GAU47808.1 hypothetical protein TSUD_404130 [Trifolium subterraneum]
Length = 810
Score = 166 bits (419), Expect = 2e-45
Identities = 77/116 (66%), Positives = 92/116 (79%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE+NGTW++ L GKK +G +WVYKIKYNSDG++ER K RLVV GN+QV +D+N+TFA
Sbjct: 467 LENNGTWTIEDLPPGKKTIGCKWVYKIKYNSDGTIERHKARLVVLGNNQVAGVDFNETFA 526
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMV VR FLAVAV++ WELH+MDVHNAFL GDL EEVYMR+PPG G V
Sbjct: 527 PVAKMVFVRVFLAVAVIRGWELHQMDVHNAFLHGDLDEEVYMRLPPGISGVSTGKV 582
>KYP66022.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 634
Score = 164 bits (415), Expect = 2e-45
Identities = 76/108 (70%), Positives = 89/108 (82%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE N TWS+ L GKKALG +WVY+IKYNSDG++ERFK RLVV GNHQ +DI+Y +TFA
Sbjct: 148 LERNNTWSMQPLPSGKKALGCKWVYRIKYNSDGTIERFKARLVVLGNHQEKDINYTETFA 207
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA KSWELH+MDVHNAFL GDL +EV++R+PPGF
Sbjct: 208 PVVKMVTVRTVLAVAAAKSWELHQMDVHNAFLHGDLHDEVFIRMPPGF 255
>GAU34891.1 hypothetical protein TSUD_144220 [Trifolium subterraneum]
Length = 1218
Score = 166 bits (419), Expect = 3e-45
Identities = 77/115 (66%), Positives = 92/115 (80%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LEDN TW++ L KKALG +WVYKIKY+SDG++ERFK RLV+ GNHQVE IDY +TFA
Sbjct: 852 LEDNNTWTMCPLPVNKKALGCKWVYKIKYHSDGTIERFKARLVILGNHQVEGIDYTETFA 911
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGL 4
VAKMVTVR L+VA K WELH+MDVHNAFL GDL EEV+M+ PPGF+ + G+
Sbjct: 912 PVAKMVTVRIVLSVAAAKRWELHQMDVHNAFLHGDLQEEVFMKPPPGFRPSQPGV 966
>CAN73272.1 hypothetical protein VITISV_013115 [Vitis vinifera]
Length = 1178
Score = 165 bits (418), Expect = 4e-45
Identities = 76/116 (65%), Positives = 94/116 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE+NGTW+L L +GK+ALG+QWVY+ KY S+G +ER K RLVV GNHQ IDY++TF+
Sbjct: 692 LEENGTWTLEPLPKGKRALGSQWVYRTKYFSNGDIERLKSRLVVLGNHQEAGIDYHETFS 751
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKM TVRAFLA+A K+WELH+MDVHNAFL GDL EEVYM++PPGF++ LV
Sbjct: 752 PVAKMTTVRAFLAIAASKNWELHQMDVHNAFLHGDLEEEVYMKLPPGFERSDPNLV 807
>KYP63761.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 1048
Score = 164 bits (416), Expect = 7e-45
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 562 LEANGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 621
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 622 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 669
>KYP43009.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 1063
Score = 164 bits (416), Expect = 7e-45
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 602 LEANGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 661
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 662 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 709
>KYP77073.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94
[Cajanus cajan]
Length = 1069
Score = 164 bits (416), Expect = 7e-45
Identities = 75/108 (69%), Positives = 88/108 (81%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE NGTWSL L +GKK LG +WVYKIK+NSDG++ER+K RLV+ GNHQ E +DY +TFA
Sbjct: 583 LEANGTWSLETLPDGKKPLGCKWVYKIKHNSDGTIERYKARLVILGNHQKEGVDYTETFA 642
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGF 25
V KMVTVR LAVA K WELH+MDVHNAFL GDL E+VYM++PPGF
Sbjct: 643 PVVKMVTVRLVLAVAATKKWELHQMDVHNAFLHGDLHEDVYMKLPPGF 690
>XP_010667912.1 PREDICTED: uncharacterized protein LOC104884908 [Beta vulgaris subsp.
vulgaris]
Length = 1117
Score = 164 bits (416), Expect = 7e-45
Identities = 77/116 (66%), Positives = 90/116 (77%)
Frame = -3
Query: 348 LEDNGTWSLVQLSEGKKALGTQWVYKIKYNSDGSVERFKGRLVVFGNHQVEDIDYNDTFA 169
LE N TW L L GK ALG +WVY+IKY+ DGS+ER+K LV+FGN QVE +DYN+T
Sbjct: 897 LEANATWVLTPLPPGKHALGCKWVYRIKYHVDGSIERYKAHLVIFGNRQVEGVDYNETLT 956
Query: 168 HVAKMVTVRAFLAVAVVKSWELHKMDVHNAFLDGDLSEEVYMRIPPGFQKGRLGLV 1
VAKMVTVRAFLA+A K+WELH+MDVHNAFL GDL EE+YM+ PPGFQ GLV
Sbjct: 957 PVAKMVTVRAFLAIAAAKNWELHQMDVHNAFLHGDLDEELYMKPPPGFQHTTSGLV 1012