BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00020644
         (1786 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_016542489.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   443   e-147
XP_018629573.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   435   e-144
XP_015059351.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   435   e-144
XP_006349816.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   435   e-144
XP_009612674.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   434   e-143
XP_019227254.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   434   e-143
XP_004252904.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   432   e-143
XP_012456061.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   430   e-142
XP_015064757.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   431   e-142
XP_009612677.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   430   e-142
XP_008390655.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   429   e-141
XP_017649963.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   429   e-141
XP_012083947.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   428   e-141
XP_009785316.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   428   e-141
CDO98863.1 unnamed protein product [Coffea canephora]                 427   e-141
XP_009773778.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   427   e-141
XP_004229242.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   426   e-140
XP_016677400.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   425   e-140
XP_019075564.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   425   e-140
XP_009352267.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   423   e-139

>XP_016542489.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X2 [Capsicum
            annuum]
          Length = 502

 Score =  443 bits (1139), Expect = e-147
 Identities = 262/520 (50%), Positives = 327/520 (62%), Gaps = 21/520 (4%)
 Frame = -2

Query: 1695 MGGLT-SNRKRVEDPFCCSPSLDLL---NFGHRSKKLKVSEVGDSNRAARVALRSSVYRS 1528
            MG LT +NRKR +D F  +    L    NF H SKK ++      +   R   +S+V R 
Sbjct: 1    MGALTCTNRKRGDDFFTSNYKTPLSVSSNFDHISKKPRLMNQIHDHPIPR---KSTVTRI 57

Query: 1527 CLYPE-VKELPRVVHAPTKNNRFALRKRSLIDKMGNFSSSLVGLRSKYAKASRESLETFR 1351
              YP  +  + R +HAP + ++      S  DKMGNF      L  +Y +A R + ET R
Sbjct: 58   FKYPSTITPIKREIHAPCRRSKIGFY--SNFDKMGNF------LAQQYDRAKRSAYETLR 109

Query: 1350 NDDVRKGEDVT-VFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLGLGVVDK 1174
               V+K ++V  + DD+                    R  G   +     R    GVV+ 
Sbjct: 110  Y--VKKDKEVIKIDDDDDEKNCGKELELENVFFAKRGRASGSGQK-----REESNGVVEI 162

Query: 1173 LIGRKRGQTVSS-----SVMNMEDLG-------LKALSLSMESDKRGK---PLHEQLLHS 1039
            +    R   VSS     +V+N  +L        L +LSLS + D       P +++LL S
Sbjct: 163  MDNSDRNFQVSSFSAVTTVLNDGNLKAENTEKMLDSLSLSGKFDDDSSSSVPSYKKLLDS 222

Query: 1038 ARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEVKRA 859
            A KR   L+ L +QI   EK+     LLR Q               V L++E+ +EV RA
Sbjct: 223  AEKRNDNLKRLQFQIEYTEKLRETQHLLRSQQKEEYVKEDVIIEPFVRLAEEEEAEVSRA 282

Query: 858  FAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKC 679
             + SNRR++LV+HE SNIDITGE LQCLRPGAWLNDEVIN+YLELLKERE R P+KFLKC
Sbjct: 283  LSNSNRRKVLVTHENSNIDITGEKLQCLRPGAWLNDEVINVYLELLKEREKREPEKFLKC 342

Query: 678  HFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKK 499
            HF NTFFYKKLI GKGGYN+Q V+RWT+QRKLGY+L +CDKIFVPIH+++HWCLAVINKK
Sbjct: 343  HFFNTFFYKKLISGKGGYNYQPVKRWTSQRKLGYSLFECDKIFVPIHKQVHWCLAVINKK 402

Query: 498  DQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCG 319
            D+KFQYLDSLGGRD+ V+KVLA Y+ DEVKDK GK+IDV SW+ EF+EDLP QENG+DCG
Sbjct: 403  DEKFQYLDSLGGRDHQVLKVLARYFIDEVKDKNGKDIDVRSWKLEFVEDLPEQENGFDCG 462

Query: 318  VFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +FM+KY DFYSR +GL FNQEHM YFR RTAKEILRLKAE
Sbjct: 463  MFMLKYTDFYSRDIGLCFNQEHMRYFRTRTAKEILRLKAE 502


>XP_018629573.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X3
            [Nicotiana tomentosiformis]
          Length = 503

 Score =  435 bits (1118), Expect = e-144
 Identities = 256/517 (49%), Positives = 322/517 (62%), Gaps = 18/517 (3%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLN---FGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LT NRKR  D F  +      N   F H SKK K+S     N       +S+V R  
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQTLNPP-----KSTVQRFL 55

Query: 1524 LYPE-VKELPRVVHAPTKNNRFALR--KRSLIDKMGNFSSSLVGLRSKYAKASRESLETF 1354
             YP+ +  + R VHAP + +RF  +    +   +MGNF S +      Y +A + +  + 
Sbjct: 56   KYPDPINPIRRQVHAPCRKSRFGSKCISANSAPEMGNFVSRV------YDEAKKSAFYSL 109

Query: 1353 RNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERD--IGKENRVCDHWRNLGLGVV 1180
            R   ++K ++V   DD+                     +  +G+++ +         G V
Sbjct: 110  RC--LKKVKEVIFIDDDDAKKGDLEAGVCEKNNFFGNLEGGVGEKSVILSQEGKETNGAV 167

Query: 1179 DKLIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRGK-PLHEQLLHSARK 1030
            + L G   G+  S     ++D  LK          L+L+  SD     PL+++LL S  K
Sbjct: 168  EILDGVD-GKDSSVVTAGLDDGNLKEESVGKMLDTLALNPTSDSAFYVPLYKKLLGSVGK 226

Query: 1029 REPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEVKRAFAI 850
               KL+ L +QI   EK L  + LLRPQ               VPL++ED +EV RA + 
Sbjct: 227  MNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDVISEPFVPLTEEDEAEVLRALSK 286

Query: 849  SNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKCHFL 670
            SNRR +LV+H+ SNIDITGE LQCLRP  WLNDEVIN+YLELLKERE R P KFLKCHF 
Sbjct: 287  SNRRRVLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKREPQKFLKCHFF 346

Query: 669  NTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKKDQK 490
            NTFFYKKLI G+GGYN+Q+VRRWT+QRKLGY L +CDKIFVPIH+EIHWCLAVINKKD+K
Sbjct: 347  NTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECDKIFVPIHKEIHWCLAVINKKDEK 406

Query: 489  FQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCGVFM 310
            FQYLDSL GRD  V+KVLA Y+ DEVKDK+GK+IDV SW++EF+EDLP Q+NG+DCGVFM
Sbjct: 407  FQYLDSLRGRDTQVLKVLARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVFM 466

Query: 309  IKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            IK ADFYSR +GL FNQE MPYFR RTAKEILRLKA+
Sbjct: 467  IKNADFYSRDIGLCFNQEDMPYFRLRTAKEILRLKAD 503


>XP_015059351.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum pennellii]
          Length = 522

 Score =  435 bits (1119), Expect = e-144
 Identities = 261/530 (49%), Positives = 325/530 (61%), Gaps = 31/530 (5%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSCLYP 1516
            MG LTSNRKR  D F  +    L N    SKKLK+S +        +A +S+V R   YP
Sbjct: 1    MGALTSNRKRGNDFFSSNSKTPLSNL---SKKLKLSVLNPP-----LASKSTVERFFKYP 52

Query: 1515 E-VKELPRVVHAPTKNNRFALR--KRSLIDKMG--NFSSSLVG--LRSKYAKASRESLET 1357
            + +  + R VHAP +  RF  +  K ++++  G   +S S +G  L  K+ +  R     
Sbjct: 53   DPINPIGREVHAPCRKLRFGSKQTKSNILNYKGVLEYSGSEMGNCLSRKHEETKRSDFYG 112

Query: 1356 FRND---------DVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIG---KENRVC 1213
             R D         D ++G       +EV                    D      E R C
Sbjct: 113  LRKDIEVIDIDGDDAKEGGSEDSSIEEVVAWGCKKSDLDIAAAANDLEDSSIEELERRGC 172

Query: 1212 DHWRNLGLGVVD--KLIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRGK 1066
            +    LG  V D  K++    G+  S     ++D  LK         +L+++ +SD    
Sbjct: 173  EKSDLLGQEVDDDVKILDGYDGKDSSVLTTGLDDENLKEESVVKMIDSLAMNPKSDSYFY 232

Query: 1065 -PLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLS 889
             PL+ +LL S  K   KL+ L +QI L EK L  ++LLRPQ               VPL+
Sbjct: 233  VPLYRKLLGSVGKISDKLKRLQFQIELNEKSLETNRLLRPQKKEEQVKEDAISEPFVPLT 292

Query: 888  DEDVSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERE 709
            +E+  EV  A + SNRR++LV+H+ SNIDITGE LQCLRPGAWLNDEVIN+YLELL+ERE
Sbjct: 293  EEEHDEVTCALSKSNRRKVLVTHKSSNIDITGEILQCLRPGAWLNDEVINVYLELLRERE 352

Query: 708  NRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEI 529
             R P KFLKCHF NTFFYKKL  G GGYN+Q+VRRWT+QRKLGY+L +CDKIFVPIH+EI
Sbjct: 353  KREPQKFLKCHFFNTFFYKKLTSGTGGYNYQSVRRWTSQRKLGYSLLECDKIFVPIHKEI 412

Query: 528  HWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDL 349
            HWCLAVINKKDQKFQYLDSL G+D NV+KVLA Y+ DEVKDK+GK IDV SW  EF+EDL
Sbjct: 413  HWCLAVINKKDQKFQYLDSLRGKDRNVLKVLATYFVDEVKDKSGKYIDVSSWMEEFVEDL 472

Query: 348  PVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            P Q+NGYDCGVFMIK ADFYSR +GL FNQ  MPYFR RTAKE+LRLKA+
Sbjct: 473  PEQKNGYDCGVFMIKNADFYSRDIGLCFNQGDMPYFRMRTAKELLRLKAD 522


>XP_006349816.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum tuberosum]
          Length = 523

 Score =  435 bits (1119), Expect = e-144
 Identities = 259/531 (48%), Positives = 324/531 (61%), Gaps = 32/531 (6%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSCLYP 1516
            MG LTSNRKR  D F  +    L N    SKKLK+S +        ++ +S+V R   YP
Sbjct: 1    MGALTSNRKRGNDFFSSNCKTPLSNL---SKKLKLSALNPP-----LSSKSTVQRFFKYP 52

Query: 1515 E-VKELPRVVHAPTKNNRFALR--KRSLIDKMG--NFSSSLVG--LRSKYAKASRESLET 1357
            + +  + R VHAP +  RF  +  K + ++  G   +S S +G  L  K+ +  R   + 
Sbjct: 53   DPINPIRREVHAPCRKLRFGSKQTKSNTVNYKGVSEYSGSEMGNCLSRKHEETKRSDFDG 112

Query: 1356 FRND---------DVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGK----ENRV 1216
             R D         D ++G       +EV                    +       E   
Sbjct: 113  LRKDIEVIDIDGDDAKEGVSEDSSIEEVEAWGCKKSDLDIAAAAANGLEDSSIEESERWG 172

Query: 1215 CDHWRNLGLGVVD--KLIGRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGK-------- 1066
            C+    LG  V D  K++    G+  S     ++D  LK  S+    +            
Sbjct: 173  CEKSDLLGQEVDDDMKILDGYDGKDSSVLTTGLDDGNLKEESVGKMIESLAMNPTPDSYF 232

Query: 1065 --PLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPL 892
              PL+++LL S  K   KL+ L +QI L EK L  ++LLRPQ               VPL
Sbjct: 233  YVPLYKKLLGSVDKISDKLKRLQFQIGLNEKSLETNRLLRPQKKEEQVIEDAISEPFVPL 292

Query: 891  SDEDVSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKER 712
            ++E+  EV  A + SNRR++LV+H+ SNIDITGE LQCLRPGAWLNDEVIN+YLELLKER
Sbjct: 293  TEEEHDEVACALSKSNRRKVLVTHKSSNIDITGEILQCLRPGAWLNDEVINVYLELLKER 352

Query: 711  ENRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQE 532
            E R P KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGY+L +CDKIFVPIH+E
Sbjct: 353  EKREPQKFLKCHFFNTFFYKKLISGTGGYNYQSVRRWTSQRKLGYSLLECDKIFVPIHKE 412

Query: 531  IHWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLED 352
            IHWCLAVINKKDQKFQYLDSL GRD NV+KVLA Y+ DEVKDK+GK+IDV SW++EF+ED
Sbjct: 413  IHWCLAVINKKDQKFQYLDSLRGRDSNVLKVLASYFVDEVKDKSGKHIDVSSWKQEFVED 472

Query: 351  LPVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            LP Q+NGYDCGVFMIK ADFYSR +GL FNQ  MPYFR RTAKE+LRLKA+
Sbjct: 473  LPEQKNGYDCGVFMIKNADFYSRDIGLCFNQGDMPYFRMRTAKELLRLKAD 523


>XP_009612674.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1
            [Nicotiana tomentosiformis]
          Length = 517

 Score =  434 bits (1117), Expect = e-143
 Identities = 258/523 (49%), Positives = 322/523 (61%), Gaps = 24/523 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLN---FGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LT NRKR  D F  +      N   F H SKK K+S     N       +S+V R  
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQTLNPP-----KSTVQRFL 55

Query: 1524 LYPE-VKELPRVVHAPTKNNRFALR--KRSLIDKMGNFSSSLVGLRSKYAKASRESLETF 1354
             YP+ +  + R VHAP + +RF  +    +   +MGNF S +     K A  S   L+  
Sbjct: 56   KYPDPINPIRRQVHAPCRKSRFGSKCISANSAPEMGNFVSRVYDEAKKSAFYSLRCLKKV 115

Query: 1353 R------NDDVRKGE-DVTVFDDEVRMXXXXXXXXXXXXXXXXERD-IGKENRVCDHWRN 1198
            +      +DD +KG+ +  V++                         +G+++ +      
Sbjct: 116  KEVIFIDDDDAKKGDLEAGVYEKSGFYGNLEAGVCEKNNFFGNLEGGVGEKSVILSQEGK 175

Query: 1197 LGLGVVDKLIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRGK-PLHEQL 1048
               G V+ L G   G+  S     ++D  LK          L+L+  SD     PL+++L
Sbjct: 176  ETNGAVEILDGVD-GKDSSVVTAGLDDGNLKEESVGKMLDTLALNPTSDSAFYVPLYKKL 234

Query: 1047 LHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEV 868
            L S  K   KL+ L +QI   EK L  + LLRPQ               VPL++ED +EV
Sbjct: 235  LGSVGKMNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDVISEPFVPLTEEDEAEV 294

Query: 867  KRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKF 688
             RA + SNRR +LV+H+ SNIDITGE LQCLRP  WLNDEVIN+YLELLKERE R P KF
Sbjct: 295  LRALSKSNRRRVLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKREPQKF 354

Query: 687  LKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVI 508
            LKCHF NTFFYKKLI G+GGYN+Q+VRRWT+QRKLGY L +CDKIFVPIH+EIHWCLAVI
Sbjct: 355  LKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECDKIFVPIHKEIHWCLAVI 414

Query: 507  NKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGY 328
            NKKD+KFQYLDSL GRD  V+KVLA Y+ DEVKDK+GK+IDV SW++EF+EDLP Q+NG+
Sbjct: 415  NKKDEKFQYLDSLRGRDTQVLKVLARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGF 474

Query: 327  DCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            DCGVFMIK ADFYSR +GL FNQE MPYFR RTAKEILRLKA+
Sbjct: 475  DCGVFMIKNADFYSRDIGLCFNQEDMPYFRLRTAKEILRLKAD 517


>XP_019227254.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Nicotiana
            attenuata] OIT31517.1 ubiquitin-like-specific protease
            esd4 [Nicotiana attenuata]
          Length = 503

 Score =  434 bits (1115), Expect = e-143
 Identities = 256/518 (49%), Positives = 319/518 (61%), Gaps = 19/518 (3%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLN---FGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LT NRKR  D F  +      N   F H SKK K+S     N       +S+V R  
Sbjct: 1    MGALTLNRKRGNDIFSSNYKTQFSNPSNFDHISKKPKLSIYQTLNPP-----KSTVQRFF 55

Query: 1524 LYPE-VKELPRVVHAPTKNNRFALRKRSL--IDKMGNFSSSLVGLRSKYAKASRESLETF 1354
             YP+ +  + R VHAP + +RF  +  S     +MGNF S +      Y +A + +  + 
Sbjct: 56   KYPDPINPIRRQVHAPCRQSRFGSKGNSANSAPEMGNFVSRV------YDEAKKSAFYSL 109

Query: 1353 RNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIG---KENRVCDHWRNLGLGV 1183
            R   ++K ++V   DD+                     + G   K   +   W+    G+
Sbjct: 110  RC--LKKVKEVIFIDDDDAKKGDLEAGVCKKSDVFCNLEGGVCEKSEFLGQKWKETN-GL 166

Query: 1182 VDKLIGRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGK----------PLHEQLLHSAR 1033
            ++ L G   G+  S     ++D  LK  S     D              PL+++LL S  
Sbjct: 167  LEILDGVD-GKDSSVVTTGLDDGNLKEESAGKMLDTLALNPTPDSAFYVPLYKKLLGSVG 225

Query: 1032 KREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEVKRAFA 853
            K   KL+ L +QI   EK L  + LLRPQ               VPL++ED +EV RA +
Sbjct: 226  KINDKLKRLQFQIGYNEKCLETYSLLRPQKKGEQVKEDVISEPFVPLTEEDEAEVSRALS 285

Query: 852  ISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKCHF 673
             SNRR +LV+H+ SNIDITGE LQCLRP  WLNDEVIN+YLELLKERE R P KFLKCHF
Sbjct: 286  KSNRRRVLVTHKNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKREPKKFLKCHF 345

Query: 672  LNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKKDQ 493
             NTFFYKKLI G+GGYN+Q+VRRWT+QRKLGY L +CDKIFVPIH+EIHWCLAVINKKD+
Sbjct: 346  FNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECDKIFVPIHKEIHWCLAVINKKDK 405

Query: 492  KFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCGVF 313
            KFQYLDSL GRD  V+KVLA Y+ DEVKDK+GK+IDV SW++EF+EDLP Q+NG+DCGVF
Sbjct: 406  KFQYLDSLRGRDTQVLKVLARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVF 465

Query: 312  MIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            MIK ADFYSR +GL FNQE+MPYFR RTAKEILRLKA+
Sbjct: 466  MIKNADFYSRDIGLCFNQENMPYFRLRTAKEILRLKAD 503


>XP_004252904.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum]
          Length = 522

 Score =  432 bits (1112), Expect = e-143
 Identities = 259/530 (48%), Positives = 325/530 (61%), Gaps = 31/530 (5%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSCLYP 1516
            MG LTSNRKR  D F  +    L N    SKKLK+S +        +A +S+V R   YP
Sbjct: 1    MGALTSNRKRGNDFFSSNSKTPLSNL---SKKLKLSALNPP-----LASKSTVERFFKYP 52

Query: 1515 E-VKELPRVVHAPTKNNRFALR--KRSLIDKMG--NFSSSLVG--LRSKYAKASRESLET 1357
            + +  + R VHAP +  RF  +  K ++++  G   +S S +G  L  K+ +  R     
Sbjct: 53   DPINPIGREVHAPCRKLRFGSKQTKSNILNYKGVLEYSGSEMGNCLSRKHEETKRGDFYG 112

Query: 1356 FRND---------DVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIG---KENRVC 1213
             + D         D ++G       +EV +                  D      E R C
Sbjct: 113  LKKDIEVIDIDGDDAKEGGSEDSSIEEVVVWGCKKSDLDIAAAANGLEDSSIEELERRGC 172

Query: 1212 DHWRNLGLGVVD--KLIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRGK 1066
            +    LG  V D  K++    G+  S     ++D  LK         +L+++ +SD    
Sbjct: 173  EKSDFLGQEVDDDVKILDGYDGKDSSVLATGLDDENLKEESVVKMIDSLAMNPKSDSYFY 232

Query: 1065 -PLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLS 889
             PL+ +LL S  K   KL+ L +QI L EK L  ++LLRPQ               VPL+
Sbjct: 233  VPLYRKLLGSVGKISDKLKRLQFQIELNEKSLETNRLLRPQKKEEQVKEDAISEPFVPLT 292

Query: 888  DEDVSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERE 709
            +++  EV  A + SNRR++LV+H+ SNIDITGE LQCLRPGAWLNDEVIN+YLELLKERE
Sbjct: 293  EKEHDEVTCALSKSNRRKVLVTHKSSNIDITGEILQCLRPGAWLNDEVINVYLELLKERE 352

Query: 708  NRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEI 529
             R P KFLKCHF NTFFYKKL  G GGYN+Q+VRRWT+QRKLGY+L +CDKIFVPIH+EI
Sbjct: 353  KREPQKFLKCHFFNTFFYKKLTSGTGGYNYQSVRRWTSQRKLGYSLLECDKIFVPIHKEI 412

Query: 528  HWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDL 349
            HWCLAVINKKDQKFQYLDSL G+D NV+KVLA Y+ DEVKDK+GK IDV SW  EF+EDL
Sbjct: 413  HWCLAVINKKDQKFQYLDSLRGKDRNVLKVLATYFVDEVKDKSGKYIDVSSWMEEFVEDL 472

Query: 348  PVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            P Q+NGYDCGVFMIK  DFYSR +GL FNQ  MPYFR RTAKE+LRLKA+
Sbjct: 473  PEQKNGYDCGVFMIKNVDFYSRDIGLCFNQGDMPYFRMRTAKELLRLKAD 522


>XP_012456061.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1
            [Gossypium raimondii] KJB72687.1 hypothetical protein
            B456_011G190600 [Gossypium raimondii]
          Length = 495

 Score =  430 bits (1106), Expect = e-142
 Identities = 251/520 (48%), Positives = 323/520 (62%), Gaps = 21/520 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVED-------------PFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARV 1555
            MG L SNRKR  D             P+  SP   L      SKK ++S + +S   A +
Sbjct: 1    MGALASNRKRYGDECFNSNFCHKKKSPYSNSPEFQL------SKKPRLSLMNESPEKANL 54

Query: 1554 ALRSSVYRSCLYPEVK-ELPRVVHAPTKNNRFALR-----KRSLIDKMGNFSSSLVGLRS 1393
            +  S+V R   YPE K   PR VHAP +  +F L      + S+   MGNF      L S
Sbjct: 55   SSNSTVLRISRYPEAKVSFPREVHAPVRRLKFGLSASKPGQDSVESYMGNF------LSS 108

Query: 1392 KYAKASRESLETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVC 1213
            + + A R++    R+    K E + V DDE +                 E+  GK N   
Sbjct: 109  RLSDARRQAWGALRHFKKEK-EVIFVEDDEEKEKGLVSDDVSVEEVELIEK--GKNNVEE 165

Query: 1212 D-HWRNLGLGVVDKLIGRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGKPLHEQLLHSA 1036
            + H++      V +L         ++  + ME   L  LSL   S+      + +L+ SA
Sbjct: 166  ERHFQPSSSSAVTEL---------NNGSLRMES-SLDMLSLREVSNGYDLEAYRKLIESA 215

Query: 1035 RKREPKLQDLSYQILLKEKMLHQHQLLRPQ-XXXXXXXXXXXXXXXVPLSDEDVSEVKRA 859
             +R  KL+DL +QI L EK +   Q LRP+                +PL++E+++ V RA
Sbjct: 216  ERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEEQHEVLPTEPFIPLTEEEMALVSRA 275

Query: 858  FAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKC 679
             ++ N R++LVSHE S+IDI GE LQCL+PGAWLNDEVINLYLELLKERENR P KFLKC
Sbjct: 276  LSVKNWRKVLVSHENSSIDIRGEILQCLKPGAWLNDEVINLYLELLKERENREPKKFLKC 335

Query: 678  HFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKK 499
            HF NTFFYKKL+  + GYN++AV+RWT+QRKLGY L DCDKIFVPIH++IHWCLAVINKK
Sbjct: 336  HFCNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYCLLDCDKIFVPIHKDIHWCLAVINKK 395

Query: 498  DQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCG 319
            DQKFQYLDSL GRD +V++ LA Y+ +EVKDK+GK+ID+ SWE+EF+EDLP QENG+DCG
Sbjct: 396  DQKFQYLDSLKGRDPHVLRALAKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCG 455

Query: 318  VFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +FM+KY DFYSRG+ L F QEHMPYFR RTAKEIL+LKA+
Sbjct: 456  MFMLKYIDFYSRGLSLCFEQEHMPYFRLRTAKEILKLKAD 495


>XP_015064757.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum pennellii]
          Length = 515

 Score =  431 bits (1107), Expect = e-142
 Identities = 254/526 (48%), Positives = 321/526 (61%), Gaps = 27/526 (5%)
 Frame = -2

Query: 1695 MGGLT-SNRKRVEDPFCCSPSLDLL---NFGHRSKKLKVSEVGDSNRAARVALRSSVYRS 1528
            MG LT S+RKR +D F  +    L    N  H SKK ++S   +  +    + +S   R 
Sbjct: 1    MGALTCSSRKRGDDFFNSNYKTPLSVSSNVDHISKKPRLSPSMNQIQDHPFSRKSIAARI 60

Query: 1527 CLYPE-VKELPRVVHAPTKNNRFALRKRSLIDKMGNFSSSLVGLRSKYAKASRESLETFR 1351
              YP  +  + R +HAP +  R     +S  DKMGNF      L  +Y +A R + ET R
Sbjct: 61   FKYPSNITPIKREIHAPCRRLRRGSNLKS--DKMGNF------LTQQYDRAKRSAFETLR 112

Query: 1350 NDDVRKGEDV-TVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLGLGVVDK 1174
               V+K ++V  + DDE+                  +  +G  +     W+    GVV+ 
Sbjct: 113  Y--VKKDKEVINIDDDEICEEGVSEDSSVKELGTGVDAKMGLASGSGLQWKESN-GVVEI 169

Query: 1173 LIGR-------KRGQTVSSSVMNMEDLG----------LKALSLSMESDKRGK----PLH 1057
            +          +  Q +SSSV+ + D G          L  LSLS + D        P +
Sbjct: 170  MDNPDAVKDMDRNFQVLSSSVVTVSDGGMLKVENAEKMLDTLSLSSKFDSASTSSSVPPY 229

Query: 1056 EQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDV 877
            ++LL SA KR   L+ L + I   EK      LLRPQ               + L +E+ 
Sbjct: 230  KKLLDSAEKRNDHLKRLQFHIEYTEKRRETQHLLRPQKKEEYVKEDVIREPFMLLDEEEE 289

Query: 876  SEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGP 697
            +EV RA + S+RR++L  HE SNIDITGE LQCLRPGAWLNDEVIN+YLELLKERE R P
Sbjct: 290  AEVSRALSNSSRRKVLAKHENSNIDITGEILQCLRPGAWLNDEVINVYLELLKERERREP 349

Query: 696  DKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCL 517
             KFLKCHF NTFFYKKLI  KGGYN+Q+V+RWT+QRKLGY L +CDKIFVPIH+++HWCL
Sbjct: 350  KKFLKCHFFNTFFYKKLISNKGGYNYQSVKRWTSQRKLGYCLFECDKIFVPIHKQVHWCL 409

Query: 516  AVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQE 337
            AVINKKD+KFQYLDSLGG D  V++VLA Y+ DEVKDK GK ID+ SW+ EF+EDLP QE
Sbjct: 410  AVINKKDEKFQYLDSLGGTDQQVLEVLARYFIDEVKDKNGKYIDINSWKLEFVEDLPEQE 469

Query: 336  NGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            NG+DCG+FM+KYADFYSR +GL F+QEHMPYFR RT KEILRLKAE
Sbjct: 470  NGFDCGMFMLKYADFYSRDIGLCFSQEHMPYFRSRTVKEILRLKAE 515


>XP_009612677.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X2
            [Nicotiana tomentosiformis]
          Length = 516

 Score =  430 bits (1105), Expect = e-142
 Identities = 258/523 (49%), Positives = 322/523 (61%), Gaps = 24/523 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLN---FGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LT NRKR  D F  +      N   F H SKK K+S     N       +S+V R  
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQTLNPP-----KSTVQRFL 55

Query: 1524 LYPE-VKELPRVVHAPTKNNRFALR--KRSLIDKMGNFSSSLVGLRSKYAKASRESLETF 1354
             YP+ +  + R VHAP + +RF  +    +   +MGNF S +     K A  S   L+  
Sbjct: 56   KYPDPINPIRRQVHAPCRKSRFGSKCISANSAPEMGNFVSRVYDEAKKSAFYSLRCLKKV 115

Query: 1353 R------NDDVRKGE-DVTVFDDEVRMXXXXXXXXXXXXXXXXERD-IGKENRVCDHWRN 1198
            +      +DD +KG+ +  V++                         +G+++ +      
Sbjct: 116  KEVIFIDDDDAKKGDLEAGVYEKSGFYGNLEAGVCEKNNFFGNLEGGVGEKSVILSQEGK 175

Query: 1197 LGLGVVDKLIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRGK-PLHEQL 1048
               G V+ L G   G+  S     ++D  LK          L+L+  SD     PL+++L
Sbjct: 176  ETNGAVEILDGVD-GKDSSVVTAGLDDGNLKEESVGKMLDTLALNPTSDSAFYVPLYKKL 234

Query: 1047 LHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEV 868
            L S  K   KL+ L +QI   EK L  + LLRPQ               VPL++ED +EV
Sbjct: 235  LGSVGKMNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDVISEPFVPLTEEDEAEV 294

Query: 867  KRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKF 688
             RA + SNRR +LV+H+ SNIDITGE LQCLRP  WLNDEVIN+YLELLKERE R P KF
Sbjct: 295  LRALSKSNRR-VLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKREPQKF 353

Query: 687  LKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVI 508
            LKCHF NTFFYKKLI G+GGYN+Q+VRRWT+QRKLGY L +CDKIFVPIH+EIHWCLAVI
Sbjct: 354  LKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECDKIFVPIHKEIHWCLAVI 413

Query: 507  NKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGY 328
            NKKD+KFQYLDSL GRD  V+KVLA Y+ DEVKDK+GK+IDV SW++EF+EDLP Q+NG+
Sbjct: 414  NKKDEKFQYLDSLRGRDTQVLKVLARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGF 473

Query: 327  DCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            DCGVFMIK ADFYSR +GL FNQE MPYFR RTAKEILRLKA+
Sbjct: 474  DCGVFMIKNADFYSRDIGLCFNQEDMPYFRLRTAKEILRLKAD 516


>XP_008390655.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Malus domestica]
          Length = 508

 Score =  429 bits (1104), Expect = e-141
 Identities = 254/518 (49%), Positives = 321/518 (61%), Gaps = 19/518 (3%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPF---CCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LTSNRKR ++     C  PSL+L NF   SK+ ++S V  S     V+ +S+  R  
Sbjct: 1    MGALTSNRKRGDERLSFNCAHPSLNLPNF-QSSKRPRLSHVNQSPNQLIVSSKSAGSRLS 59

Query: 1524 LYPEVKE-LPRVVHAPTKNNRFALRKRSLIDKMGNFSSSLVG--LRSKYAKASRESLETF 1354
             YP++K  LPRV HAP +  +F   +       G      +G  L     KA   +   F
Sbjct: 60   RYPDIKPPLPRV-HAPCRTQKFGSFRXLSPKTRGVPEERSMGNVLSYHLDKAKNVAFSAF 118

Query: 1353 RNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLGLGVVDK 1174
            R     K ++V   D EV                  E D  +   V D+ + L   +VD 
Sbjct: 119  RYS--TKDKEVIELDKEVE-DDRVSEDSSIEEVVAIEEDDQEGPSVVDYGQELDTKMVD- 174

Query: 1173 LIGRKRGQTVSSSVMN----------MEDLG---LKALSLSMESDKRGKPLHEQLLHSAR 1033
              G +     +SSV++           ED     L +LSL+ E     K  ++ L+ S  
Sbjct: 175  -CGTQATHPSASSVVSDLTTTTNTAFKEDSAGKMLDSLSLNREVGVPSKAAYKLLIESLE 233

Query: 1032 KREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEVKRAFA 853
            +R PKL+ L +QI    K  +  + LRPQ               VPL  E+V+E+++AF+
Sbjct: 234  RRTPKLKHLDFQIDFHWKKRYMLESLRPQ---KKPVEVVPVEPFVPLRKEEVAEIEQAFS 290

Query: 852  ISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKCHF 673
             SNR+ ILV+HE SNI+ITGE LQCLRPGAWL DEVIN+YLELLKERE R P KFLKCHF
Sbjct: 291  SSNRKRILVTHENSNIEITGELLQCLRPGAWLKDEVINVYLELLKEREKREPQKFLKCHF 350

Query: 672  LNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKKDQ 493
             NTFFY KLI GKGGY++++VRRWTT+RKLGY L DCDKIFVPIH+EIHWCLAVINK DQ
Sbjct: 351  FNTFFYTKLISGKGGYDYKSVRRWTTERKLGYYLIDCDKIFVPIHKEIHWCLAVINKVDQ 410

Query: 492  KFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCGVF 313
            K QYLDSL GRD  VM++LA YY DEVKDK+G++ID+ SWE E++++LP QENGYDCGVF
Sbjct: 411  KLQYLDSLKGRDTQVMRILAKYYVDEVKDKSGEDIDLSSWELEYVDNLPEQENGYDCGVF 470

Query: 312  MIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            M+KYADFYSR +GL F QEHMPYFR RTAKEIL+L+A+
Sbjct: 471  MVKYADFYSRDIGLCFKQEHMPYFRSRTAKEILKLRAD 508


>XP_017649963.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Gossypium arboreum]
            KHG04279.1 Ubiquitin-like-specific protease ESD4
            [Gossypium arboreum]
          Length = 495

 Score =  429 bits (1102), Expect = e-141
 Identities = 251/520 (48%), Positives = 320/520 (61%), Gaps = 21/520 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVED-------------PFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARV 1555
            MG L SNRKR  D             P+  SP   L      SKK ++S + +S   A +
Sbjct: 1    MGALASNRKRYGDECFNSNFCHKKKSPYSNSPEFQL------SKKRRLSLMNESPEKANL 54

Query: 1554 ALRSSVYRSCLYPEVK-ELPRVVHAPTKNNRFALR-----KRSLIDKMGNFSSSLVGLRS 1393
            +  S+V R   YPE K   PR VHAP +  +F L      + S+   MGNF      L S
Sbjct: 55   SSNSTVLRISRYPESKFSFPREVHAPVRRLKFWLSASKPGQDSVESYMGNF------LSS 108

Query: 1392 KYAKASRESLETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKEN-RV 1216
            + + A R++    R+    K E + V DDE +                 E+  GK N + 
Sbjct: 109  RLSDAKRQAWGALRHFKKEK-EVIFVEDDEEKEKGLVSDDVSVEEVELIEK--GKNNVKE 165

Query: 1215 CDHWRNLGLGVVDKLIGRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGKPLHEQLLHSA 1036
              H++      V +L         ++  + ME   L  LSL   S+      + +L+ SA
Sbjct: 166  ERHFQPSSSSAVTEL---------NNGSLRMES-SLDMLSLREVSNGYDLEAYRKLIESA 215

Query: 1035 RKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXV-PLSDEDVSEVKRA 859
             +R  KL+DL +QI L EK +   Q LRP+                 PL++E+++ V RA
Sbjct: 216  ERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEEQHEVLPTEPFIPLTEEEMALVSRA 275

Query: 858  FAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKC 679
             +  N R++LVSHE S+IDI GE LQCL+PGAWLNDEVINLYLELLKERENR P KFLKC
Sbjct: 276  LSAKNWRKVLVSHENSSIDIRGEILQCLKPGAWLNDEVINLYLELLKERENREPKKFLKC 335

Query: 678  HFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKK 499
            HF NTFFYKKL+  + GYN++AV+RWT+QRKLGY L DCDKIFVPIH++IHWCLAVINKK
Sbjct: 336  HFFNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYCLLDCDKIFVPIHKDIHWCLAVINKK 395

Query: 498  DQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCG 319
            DQKFQYLDSL GRD NV++ LA Y+ +EVKDK+GK+ID+ SWE+EF+EDLP QENG+DCG
Sbjct: 396  DQKFQYLDSLKGRDPNVLRALAKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCG 455

Query: 318  VFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +FM+KY DFYSRG+ L F QEHMPYFR RT KEIL+LKA+
Sbjct: 456  MFMLKYIDFYSRGLSLCFEQEHMPYFRLRTTKEILKLKAD 495


>XP_012083947.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Jatropha curcas]
            KDP27803.1 hypothetical protein JCGZ_18883 [Jatropha
            curcas]
          Length = 497

 Score =  428 bits (1101), Expect = e-141
 Identities = 251/516 (48%), Positives = 326/516 (63%), Gaps = 17/516 (3%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSS---VYRSC 1525
            MG LTSN KR  DP+  +P        H SKK ++S     ++A    L SS   V R  
Sbjct: 1    MGALTSNSKR--DPYSNAPDF------HISKKPRLSSF--MHQAPNQTLGSSNNTVSRIS 50

Query: 1524 LYPEV-KELPRVVHAPTKNNRFAL----------RKRSLIDKMGNFSSSLVGLRSKYAKA 1378
             YPE   +  R VHAP +  +F +          + +S   +MGNF S  +        A
Sbjct: 51   RYPETTSKFRREVHAPCRILKFGISRSKDIEYSEKNKSSTAEMGNFLSKQLDF------A 104

Query: 1377 SRESLETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRN 1198
             R +L  FR   +++ E + V ++  +                   + G+E+R     R 
Sbjct: 105  KRSALGAFRYL-IKEKEVIDVDNELEKSGKEVVSDDSSIQEVEAIEEDGREDRSMVFDRR 163

Query: 1197 LGLGVVD-KLIGRKRGQTVSSSV-MNMEDLGLKALSLSMESDKRGKPLHEQLLHSARKRE 1024
             G GVVD K++  +   TV  ++ +      L +L+L+ E        +++LL  A +R+
Sbjct: 164  SGEGVVDVKIVEERSVVTVDGNIGLQYASKMLDSLTLNNEVVVSSVEAYKKLLGMAERRD 223

Query: 1023 PKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSEVKRAFAISN 844
             +L++L +QI L EK L  +Q +RP                +PL++E+  ++KRA + +N
Sbjct: 224  GRLKELDFQIELNEKKLSFYQSIRP--VKKPEVQEIPDEPFIPLTEEEEYDIKRALSANN 281

Query: 843  RREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKCHFLNT 664
            RR ILV+H+ SNIDITGE LQCL PGAWLNDEVINLY ELLKERE R P+KFLKCHF NT
Sbjct: 282  RRRILVTHKNSNIDITGEILQCLAPGAWLNDEVINLYFELLKEREKREPEKFLKCHFFNT 341

Query: 663  FFYKKLIGG-KGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKKDQKF 487
            FFYKKL GG K  Y+++AVRRWTT+RKLGY L DCDKIFVP+H+EIHWCLA+INK+DQKF
Sbjct: 342  FFYKKLAGGDKKIYDYKAVRRWTTERKLGYFLIDCDKIFVPVHREIHWCLAIINKRDQKF 401

Query: 486  QYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCGVFMI 307
            QYLDSL GRD  V++ LA YY +EVKDK+ K+IDV +WEREFLEDLP Q+NGYDCGVFMI
Sbjct: 402  QYLDSLKGRDLRVLENLAKYYVEEVKDKSKKDIDVSNWEREFLEDLPEQQNGYDCGVFMI 461

Query: 306  KYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            KYADFYSRG+GL FNQ+HMPYFR RTAKEILRL+A+
Sbjct: 462  KYADFYSRGIGLCFNQDHMPYFRMRTAKEILRLRAD 497


>XP_009785316.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X4
            [Nicotiana sylvestris]
          Length = 491

 Score =  428 bits (1100), Expect = e-141
 Identities = 261/531 (49%), Positives = 326/531 (61%), Gaps = 32/531 (6%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFG-HRSKKLKVS---EVGDSNRAARVALRSSVYRS 1528
            MG LTS RKR +D F  +    + N   H SKK ++S      D ++ + V    + Y S
Sbjct: 1    MGALTSYRKRGDDFFTLNYKTPVSNVDPHISKKPRLSMNQTTQDHSKTSTVTRLFTKYPS 60

Query: 1527 CLYPEVKELPRVVHAPTK---NNRFALRKRSLIDKMGNFSSSLVGLRSKYAKASRESLET 1357
             + P    + R +HAP +   ++RF     S+   MGNF      +R  Y +A R + +T
Sbjct: 61   SITP----IKREIHAPCRRPSSSRFGGGSHSI---MGNFL-----VRQLY-RAKRSAFKT 107

Query: 1356 FRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLGLGVVD 1177
            FR    +  E V   DD+                     D G  N        LG GVV 
Sbjct: 108  FRYVKKKDKEVVINIDDD---------------------DDGDSNV-----EELGTGVVP 141

Query: 1176 KL-----IGRKRGQTVSSSVMNMEDLGLKAL----------SLSMESDKRGK-------- 1066
            K       G++R +  SSSV+ + D  +  L          SLS+ S K           
Sbjct: 142  KTGLFDGSGQRR-KLQSSSVVTVSDDDVNLLKVENAEKMMDSLSLSSSKFDDSSSSASFV 200

Query: 1065 PLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSD 886
            P +++LL SA KR   L+ L + I   EK+L  ++LLRPQ                   D
Sbjct: 201  PSYKKLLDSAEKRNNSLKSLQFHIEYTEKILETYKLLRPQKKDEQYVKEDVITEPFVQID 260

Query: 885  -EDVSEVKRAFA-ISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKER 712
             E+ +EV  A +  S+RR++LV+HE SNIDITGE LQCLRPGAWLNDEVIN+YLELLKER
Sbjct: 261  KEEEAEVSHALSNYSSRRKVLVTHENSNIDITGEILQCLRPGAWLNDEVINVYLELLKER 320

Query: 711  ENRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQE 532
            E R P KFL+CHF NTFFYKKLI GKGGYN+Q+V+RWT+QRKLGY+L +CDKIFVPIHQE
Sbjct: 321  EKREPQKFLRCHFFNTFFYKKLISGKGGYNYQSVKRWTSQRKLGYSLFECDKIFVPIHQE 380

Query: 531  IHWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLED 352
            +HWCLAVINKKD+KFQYLDSLGGRD  V++VL  Y+ DEVKDK GK+IDV SW++EF+ED
Sbjct: 381  VHWCLAVINKKDEKFQYLDSLGGRDNQVLRVLTRYFIDEVKDKNGKDIDVSSWKQEFVED 440

Query: 351  LPVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            LP QEN +DCG+FMIKYADFYSR +GLRF+QEHMPYFR RTAKEILRLKAE
Sbjct: 441  LPEQENWFDCGMFMIKYADFYSRDIGLRFSQEHMPYFRLRTAKEILRLKAE 491


>CDO98863.1 unnamed protein product [Coffea canephora]
          Length = 511

 Score =  427 bits (1099), Expect = e-141
 Identities = 254/530 (47%), Positives = 319/530 (60%), Gaps = 31/530 (5%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSCLYP 1516
            MG LTSNRKR  D         L++    SKK ++     +    R    ++V++   YP
Sbjct: 1    MGALTSNRKRGYDFSSSVNYGSLVSNSPVSKKSRLP-ASINQTTGRTDRITAVFQFFRYP 59

Query: 1515 EVKEL-PRVVHAPTKNNRFALR------------KRSLIDKMGNFSSSLVGLRSKYAKAS 1375
              K L  R  HAP + +R                K    D+MGN       L  +Y    
Sbjct: 60   VEKTLFKREPHAPVRRHRGVSSVNLGNSASKVSYKVGSADEMGNL------LSGQYKSTK 113

Query: 1374 RESLETFR-NDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRN 1198
            R +L+T R N++ +K  +V    +EV+                       E R    W++
Sbjct: 114  RSALDTLRFNEEDKKVTEVA--KEEVQEVSEDSSIEEVEIL---------EVREDQKWKD 162

Query: 1197 LGLGVVDK--------LIGRKRGQTVSSSVMNMEDLGLK---------ALSLSMESDKRG 1069
             G GVVD+         + +    + SS++ N+ D  LK         +LSLS E     
Sbjct: 163  -GNGVVDEDSRKFDGIAVDKDSRPSSSSAMTNVSDGILKVETTEKLLESLSLSQELGVPQ 221

Query: 1068 KPLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLS 889
            + +H++LL+ A +R  K+  L++QI   EK L   QLLRPQ                PL+
Sbjct: 222  ESVHKKLLYVAERRNDKINSLNFQIEYTEKQLQLQQLLRPQKKEEAAKKDVTAEAFKPLT 281

Query: 888  DEDVSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERE 709
            +E+ +EV RA + S+RR++LV+HE SNI ITGE LQCLRP AWLNDEVINLYLELLKERE
Sbjct: 282  EEEETEVNRALSNSSRRKLLVTHENSNISITGEVLQCLRPRAWLNDEVINLYLELLKERE 341

Query: 708  NRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEI 529
             R P KFL CHF NTFF+KKL+ GKGGYN+Q+VRRWT+Q+KLGY + DCDKIFVPIH+E+
Sbjct: 342  RREPKKFLNCHFFNTFFFKKLVSGKGGYNYQSVRRWTSQKKLGYCIFDCDKIFVPIHKEV 401

Query: 528  HWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDL 349
            HWCLAVINKKD+KFQYLDSLGG D  V+KVLA Y  DEVKDK GK IDV SW++EF+ DL
Sbjct: 402  HWCLAVINKKDEKFQYLDSLGGVDSQVIKVLARYIVDEVKDKCGKGIDVSSWQQEFVADL 461

Query: 348  PVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            P QENG+DCGVFMIKYADFYSR +GL FNQEHMPYFR RTA EILR KAE
Sbjct: 462  PEQENGFDCGVFMIKYADFYSRDIGLCFNQEHMPYFRLRTALEILRSKAE 511


>XP_009773778.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Nicotiana
            sylvestris] XP_016461419.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Nicotiana tabacum]
          Length = 503

 Score =  427 bits (1098), Expect = e-141
 Identities = 259/524 (49%), Positives = 320/524 (61%), Gaps = 25/524 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCC-------SPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSV 1537
            M  LT NRKR  D F         SPS    NF H SKK K+S     N       +S+V
Sbjct: 1    MVALTFNRKRGNDFFSSNYKTQFSSPS----NFDHISKKPKLSIYQTLNPP-----KSTV 51

Query: 1536 YRSCLYPE-VKELPRVVHAPTKNNRFALRKRSL--IDKMGNFSSSLVGLRSKYAKASRES 1366
             R  +YP+ +  + R VHAP +++RF  +  S     + GNF S +      Y +A + +
Sbjct: 52   QRFFMYPDPINPIRRQVHAPCRHSRFGSKGISANSAPETGNFVSRV------YDEAKKSA 105

Query: 1365 LETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIG---KENRVCDHWRNL 1195
              + R   ++K ++V   DD+                     + G   K + +   W+  
Sbjct: 106  FYSLRC--LKKVKEVIFIDDDDAKKGDLEAGVCKQSDVFCNLEGGVCEKSDFLGQKWKQT 163

Query: 1194 --------GLGVVDKLI---GRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGK-PLHEQ 1051
                    G+   D L+   G   G     SV  M D     L+L+  SD     PL+++
Sbjct: 164  NGPLEILNGVDGHDSLVVTTGLDDGNLKEESVGKMLD----TLALNPTSDSAFYVPLYKK 219

Query: 1050 LLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSE 871
            LL S  K   KL+ L +QI   EK L  + LLRPQ               VPL++E+  E
Sbjct: 220  LLGSVGKINDKLKRLQFQIGYNEKCLETYSLLRPQKKEEQVKEDVISEPFVPLTEEEEVE 279

Query: 870  VKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDK 691
            V RA + SNRR +LV+H+ SNIDITGE LQCLRP  WLNDEVIN+YLELLKERE R P K
Sbjct: 280  VSRALSKSNRRRVLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKRDPQK 339

Query: 690  FLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAV 511
            FLKCHF NTFFYKKLIGG+GGYN+Q+VRRWT+QRKL Y L +CDKIFVPIH+EIHWCLAV
Sbjct: 340  FLKCHFFNTFFYKKLIGGRGGYNYQSVRRWTSQRKLDYCLLECDKIFVPIHKEIHWCLAV 399

Query: 510  INKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENG 331
            INKKD+KFQYLDSL GRD  V+KVLA Y+ DEVKDK GK+IDV SW++EF+EDLP Q+NG
Sbjct: 400  INKKDKKFQYLDSLRGRDTQVLKVLARYFVDEVKDKNGKDIDVSSWKQEFVEDLPEQKNG 459

Query: 330  YDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +DCGVFMIK ADFYSR +GL FNQE MPYFR RTAKEILRLKA+
Sbjct: 460  FDCGVFMIKNADFYSRDIGLCFNQEDMPYFRLRTAKEILRLKAD 503


>XP_004229242.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum] XP_010321351.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum] XP_010321353.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum]
          Length = 515

 Score =  426 bits (1095), Expect = e-140
 Identities = 253/527 (48%), Positives = 320/527 (60%), Gaps = 28/527 (5%)
 Frame = -2

Query: 1695 MGGLT-SNRKRVEDPFCCSPSLDLL---NFGHRSKKLKVSEVGDSNRAARVALRSSVYRS 1528
            MG LT S+RKR +D F  +    L    N  H SKK ++S   +  +    + +S   R 
Sbjct: 1    MGALTCSSRKRGDDFFNSNYKTPLSVSSNVDHISKKPRLSPSMNQIQDHPFSRKSIAARI 60

Query: 1527 CLYPE-VKELPRVVHAPTKNNRFALRKRSLIDKMGNFSSSLVGLRSKYAKASRESLETFR 1351
              YP  +  + R +HAP +  R     +S  DKMGNF      L  +Y +A R + ET R
Sbjct: 61   FKYPSNITPIKREIHAPCRRLRRGSNLKS--DKMGNF------LTQQYDRAKRSAFETLR 112

Query: 1350 NDDVRKGEDV-TVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLGLGVVDK 1174
               V+K ++V  + DDE+                  +   G  +     W+    GVV+ 
Sbjct: 113  Y--VKKDKEVINIDDDEICEEGVSEDSSVKELGTGVDAKTGLASGSGSQWKESN-GVVEI 169

Query: 1173 LIGR-------KRGQTVSSSVMNMEDLGLK-----------ALSLSMESDKRGK----PL 1060
            +          +  Q +SSSV+ + D G+K            LSLS + D        P 
Sbjct: 170  MDNPDAVKDMDRNFQVLSSSVVTVSD-GVKLKVENAEKMLDTLSLSSKFDSASTSSSVPP 228

Query: 1059 HEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDED 880
            +++LL SA KR   L+ L + I   EK      LLRPQ               + L +E+
Sbjct: 229  YKKLLGSAEKRNDHLKRLQFHIEYTEKRRETQHLLRPQKKEEYVKEDVITEPFMLLDEEE 288

Query: 879  VSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRG 700
             +EV RA + S+RR++L  HE SNIDITGE LQCLRPGAWLNDEVIN+YLELLKERE R 
Sbjct: 289  EAEVSRALSNSSRRKVLAKHENSNIDITGEILQCLRPGAWLNDEVINVYLELLKERERRE 348

Query: 699  PDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWC 520
            P KFLKCHF NTFFYKKLI  KG YN+Q+V+RWT+QRKLGY L +CDKIFVPIH+++HWC
Sbjct: 349  PKKFLKCHFFNTFFYKKLISNKGDYNYQSVKRWTSQRKLGYCLFECDKIFVPIHKQVHWC 408

Query: 519  LAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQ 340
            LAVINKKD+KFQYLDSLGG D  V++VLA Y+ DEVKDK GK ID+ SW+ EF+EDLP Q
Sbjct: 409  LAVINKKDEKFQYLDSLGGTDQQVLEVLARYFIDEVKDKNGKYIDINSWKLEFVEDLPEQ 468

Query: 339  ENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            ENG+DCG+FM+KYADFYSR +GL F+QEHMPYFR RT KEILRLKAE
Sbjct: 469  ENGFDCGMFMLKYADFYSRDIGLCFSQEHMPYFRSRTVKEILRLKAE 515


>XP_016677400.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Gossypium hirsutum]
          Length = 495

 Score =  425 bits (1093), Expect = e-140
 Identities = 250/520 (48%), Positives = 319/520 (61%), Gaps = 21/520 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVED-------------PFCCSPSLDLLNFGHRSKKLKVSEVGDSNRAARV 1555
            MG L SNRKR  D             P+  SP   L      SKK ++S + +S   A +
Sbjct: 1    MGALASNRKRYGDECFNSNFCHKKKSPYSNSPEFQL------SKKRRLSLMNESPEKANL 54

Query: 1554 ALRSSVYRSCLYPEVK-ELPRVVHAPTKNNRFALR-----KRSLIDKMGNFSSSLVGLRS 1393
            +  S+V R   YPE K   PR VHAP +  +F L      + S+   MGNF      L S
Sbjct: 55   SSNSTVLRISRYPESKFSFPREVHAPVRRLKFWLSASKPGQDSVESYMGNF------LSS 108

Query: 1392 KYAKASRESLETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKEN-RV 1216
            + + A R++    R+    K E + V DDE +                 E+  GK N + 
Sbjct: 109  RLSDAKRQAWGALRHFKKEK-EVIFVEDDEEKEKGLVSDDVSVEEVELIEK--GKNNVKE 165

Query: 1215 CDHWRNLGLGVVDKLIGRKRGQTVSSSVMNMEDLGLKALSLSMESDKRGKPLHEQLLHSA 1036
              H++      V +L         ++  + ME   L  LSL   S+      + +L+ SA
Sbjct: 166  ERHFQPSSSSAVTEL---------NNGSLRMES-SLDMLSLREVSNGYDLEAYRKLIESA 215

Query: 1035 RKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXV-PLSDEDVSEVKRA 859
             +R  KL+DL +QI L EK +   Q LRP+                 PL++E+++ V RA
Sbjct: 216  ERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEEQHEVLPTEPFIPLTEEEMALVSRA 275

Query: 858  FAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDKFLKC 679
             +  N R++LVSHE S+IDI GE LQCL+PGAWLNDEVINLYLELLKERENR P KFLKC
Sbjct: 276  LSAKNWRKVLVSHENSSIDIRGEILQCLKPGAWLNDEVINLYLELLKERENREPKKFLKC 335

Query: 678  HFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAVINKK 499
            HF NTFFYKKL+  + GYN++AV+RWT+QRKLGY L DCDKIFVPIH++IHWCLAVINKK
Sbjct: 336  HFFNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYCLLDCDKIFVPIHKDIHWCLAVINKK 395

Query: 498  DQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENGYDCG 319
             QKFQYLDSL GRD NV++ LA Y+ +EVKDK+GK+ID+ SWE+EF+EDLP QENG+DCG
Sbjct: 396  YQKFQYLDSLKGRDPNVLRALAKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCG 455

Query: 318  VFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +FM+KY DFYSRG+ L F QEHMPYFR RT KEIL+LKA+
Sbjct: 456  MFMLKYIDFYSRGLSLCFEQEHMPYFRLRTTKEILKLKAD 495


>XP_019075564.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1 [Vitis
            vinifera]
          Length = 522

 Score =  425 bits (1093), Expect = e-140
 Identities = 262/532 (49%), Positives = 331/532 (62%), Gaps = 33/532 (6%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPFCCSPS--------LDLLNFGHRSKKLKVSEVGDSNRAARVALRSS 1540
            MG LTSNRKR ++ F  + +        LD     H SKK ++S +  +   A V+ RS+
Sbjct: 1    MGALTSNRKRGDECFTLNHTFLSPYPQDLDRRIDSHISKKPRLSSMPQTPDPA-VSSRST 59

Query: 1539 VYRSCLYPE-VKELPRVVHAPTK----------NNRFALRKRSLIDKMGNFSSSLVG--L 1399
              R   YPE   +L R VHAP +          N    LR   + +K+G   S+++G  L
Sbjct: 60   ASRIQRYPEPTAKLRREVHAPCRVVKFGFAASSNRESRLRIGEVDEKVG--LSNVMGNVL 117

Query: 1398 RSKYAKASR---ESLETFRND----DV--RKGEDVTVFDDEVRMXXXXXXXXXXXXXXXX 1246
             S+Y KA     ++L  FR D    DV   +GED+   D  +                  
Sbjct: 118  SSQYEKAKNTAIQALRYFRKDKEVIDVGNERGEDLASEDSSIEEIEAVEDGREGRSVVSD 177

Query: 1245 ERDIGKENRVCDHWRNLGLGVVDKLIGRKRGQTVSSSVMNMEDLGLKAL-SLSM-ESD-K 1075
            ER  G +       + L      K   R+  Q+ SS V  + ++ LK L SL++ E+D +
Sbjct: 178  ERSRGADGAAVPDIQELET----KNFDRRLWQSSSSGVTELSNVNLKMLDSLTLRETDVE 233

Query: 1074 RGKPLHEQLLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVP 895
             G   H++ L SA KR  KL+DLS QI   E        LRP                 P
Sbjct: 234  LGVLPHKKWLDSAEKRNSKLRDLSVQIKFLEAQRSSLHALRPAKKPEEEVPREPFL---P 290

Query: 894  LSDEDVSEVKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKE 715
            L+ E+ +EV RA +  NRR++LV+HE SNI+ITGE LQCL+P AWLNDEVIN+YLELLKE
Sbjct: 291  LTQEEEAEVDRALSSINRRKVLVTHEISNIEITGEILQCLQPTAWLNDEVINVYLELLKE 350

Query: 714  RENRGPDKFLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQ 535
            RE R P KFLKCHF NTFFYKKLI G+  Y++++VRRWTTQRKLGY+LS+CDKIFVPIHQ
Sbjct: 351  REKREPKKFLKCHFFNTFFYKKLISGRNSYDYKSVRRWTTQRKLGYSLSECDKIFVPIHQ 410

Query: 534  EIHWCLAVINKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLE 355
            EIHWCLAVINK+D+KFQYLDSL G D  V+KVLA YY DEVKDK+ K+ID+ SWE+E++E
Sbjct: 411  EIHWCLAVINKQDKKFQYLDSLKGMDTRVLKVLARYYVDEVKDKSEKDIDLSSWEQEYVE 470

Query: 354  DLPVQENGYDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            DLP Q+NGYDCG+FMIKYADFYSRG+ L FNQEHMPYFR RTAKEIL+LKA+
Sbjct: 471  DLPEQKNGYDCGMFMIKYADFYSRGIELCFNQEHMPYFRLRTAKEILKLKAD 522


>XP_009352267.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Pyrus x
            bretschneideri]
          Length = 508

 Score =  423 bits (1088), Expect = e-139
 Identities = 251/524 (47%), Positives = 325/524 (62%), Gaps = 25/524 (4%)
 Frame = -2

Query: 1695 MGGLTSNRKRVEDPF---CCSPSLDLLNFGHRSKKLKVSEVGDSNRAARVALRSSVYRSC 1525
            MG LT+NRKR ++     C  PS +L NF   SK+ ++S V  S     V+ +S+  R  
Sbjct: 1    MGALTNNRKRGDECLSFSCAHPSPNLPNF-QSSKRPRLSHVNQSPNQLIVSSKSASSRLS 59

Query: 1524 LYPEVKE-LPRVVHAPTKNNRFAL------RKRSLIDK--MGNFSSSLVGLRSKYAKASR 1372
             YP++K  LPRV HAP +  +F        + R + ++  MGN  S+      +  KA  
Sbjct: 60   RYPDIKPPLPRV-HAPCRTQKFGSFRGLSPKTRGVPEERSMGNVPSN------RLDKAKN 112

Query: 1371 ESLETFRNDDVRKGEDVTVFDDEVRMXXXXXXXXXXXXXXXXERDIGKENRVCDHWRNLG 1192
             +   FR     K ++V   D EV                  E D  +   V D+ + L 
Sbjct: 113  AAFGAFRYSS--KDKEVIELDKEVE-DDRVSEDSSIEEVVAIEEDDQEGPSVVDYGQELD 169

Query: 1191 LGVVDKLIGRKRGQTVSSSVMN----------MEDLG---LKALSLSMESDKRGKPLHEQ 1051
              +VD   G +     +SSV++           ED     L +L+L+ E+    K  ++ 
Sbjct: 170  TKMVD--YGTQATHPSASSVVSDLTTTTNTAFKEDSAGKMLDSLTLNREAGVPSKAAYKL 227

Query: 1050 LLHSARKREPKLQDLSYQILLKEKMLHQHQLLRPQXXXXXXXXXXXXXXXVPLSDEDVSE 871
            L+ S  +R PKL+ L +QI    K     + LRPQ                PL  E+V+E
Sbjct: 228  LIESVERRAPKLKYLDFQIDFHWKKRSMLESLRPQKKPVEVVPVEPFV---PLRKEEVAE 284

Query: 870  VKRAFAISNRREILVSHEKSNIDITGETLQCLRPGAWLNDEVINLYLELLKERENRGPDK 691
            +++AF+ SNR+ ILV+HE SNI+ITGE LQCLRPGAWL DEVIN+YLELLKERE R P K
Sbjct: 285  IEQAFSSSNRKRILVTHENSNIEITGELLQCLRPGAWLKDEVINVYLELLKEREKREPQK 344

Query: 690  FLKCHFLNTFFYKKLIGGKGGYNFQAVRRWTTQRKLGYTLSDCDKIFVPIHQEIHWCLAV 511
            FLKCHF NTFFY KLI GKGGY++++VRRWTT+RKLGY L DCDKIFVPIH+EIHWCLAV
Sbjct: 345  FLKCHFFNTFFYTKLISGKGGYDYKSVRRWTTERKLGYYLIDCDKIFVPIHKEIHWCLAV 404

Query: 510  INKKDQKFQYLDSLGGRDYNVMKVLAMYYADEVKDKTGKNIDVGSWEREFLEDLPVQENG 331
            INK DQK QYLDSL GRD  VM++LA YY DEVKDK+G++ID+ SWE E++++LP QENG
Sbjct: 405  INKVDQKLQYLDSLKGRDTQVMRILAKYYVDEVKDKSGEDIDLSSWELEYVDNLPEQENG 464

Query: 330  YDCGVFMIKYADFYSRGVGLRFNQEHMPYFRQRTAKEILRLKAE 199
            +DCGVFM+KYADFYSR +GL F QEHMPYFR RTAKEIL+L+A+
Sbjct: 465  FDCGVFMVKYADFYSRDIGLCFKQEHMPYFRLRTAKEILKLRAD 508