BLASTX nr result
ID: Lithospermum23_contig00019971
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00019971
(1652 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_006358915.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 509 e-174
XP_015085140.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 505 e-172
XP_015085141.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 504 e-172
XP_006358870.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 504 e-172
XP_004245646.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 503 e-171
XP_016502026.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 496 e-168
XP_006358914.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 496 e-168
XP_019179544.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 494 e-167
XP_019070768.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 492 e-167
XP_016567613.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 492 e-167
XP_016567623.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 491 e-167
XP_016567604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 488 e-165
XP_009609252.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 485 e-164
XP_019238293.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 485 e-164
XP_016469425.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 480 e-163
OIT21830.1 cytochrome p450 76c2 [Nicotiana attenuata] 479 e-163
XP_010325430.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 478 e-162
XP_004245647.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 478 e-161
XP_010053332.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 469 e-158
XP_015896285.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase ... 465 e-156
>XP_006358915.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum tuberosum]
Length = 495
Score = 509 bits (1311), Expect = e-174
Identities = 242/450 (53%), Positives = 341/450 (75%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI +GK+PH ++ N+A+ YGP+M+ KLG K L+VGSSP A+EIL D ILSGRD P
Sbjct: 46 NIFQMGKMPHITLTNFAKTYGPLMSLKLGTKWLVVGSSPLAAVEILKTHDKILSGRDVPS 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P +L+ S+GW +CN W+ LR LC++ELF+GK LESQ LREKK+ ++V+FL+
Sbjct: 106 AAPSKGSDLDKTSLGWISECNSGWRYLRTLCRTELFAGKVLESQACLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT+ NMLSN+L+S+D+++L+E+IE +K+L+RGIVEV+SAPN+SD
Sbjct: 166 SKEGQVVNIGELVFATVLNMLSNVLISKDMVNLEEEIEDGVIKSLVRGIVEVLSAPNISD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHE--ANQRDFLDTLIDQKFSD 727
+YPI +LD QGL+ K+ L +L S PI+EERR + + ++Q+DFL+TL+D F++
Sbjct: 226 LYPILGKLDLQGLQKKSIDLVTKLCSNWEPILEERRNSREKGSSSQQDFLETLLDNGFTN 285
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++LMEL +AG+DTSTSTI+W MAEL+KN E MKK++EEL+IE+ + +KES ++
Sbjct: 286 DRIHQLLMELLSAGSDTSTSTIEWAMAELMKNVESMKKVQEELEIELSESDYLKESQLLQ 345
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KET +E C+VM+YTIPK+AQ++VN+WAI RDP++WE P
Sbjct: 346 MSYIQACVKETLRLHPPGPLLLPHRAIESCQVMSYTIPKDAQILVNIWAITRDPLIWEEP 405
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF++S +DF G DF +LPFGAGRR+CP PMAA +I LV+ASLVH FDW LP G
Sbjct: 406 DMFRPQRFLSSDMDFKGNDFEYLPFGAGRRICPALPMAAIKIPLVLASLVHFFDWELPCG 465
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ P LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 466 KCPVELDMTEKFGVTLQKKEPLLLIPKARK 495
>XP_015085140.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum pennellii]
Length = 495
Score = 505 bits (1301), Expect = e-172
Identities = 238/450 (52%), Positives = 340/450 (75%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+IYGP+M+ +LG + L+VGSS + A+EIL D ILSGR P
Sbjct: 46 NIFHMGKMPHITLTNFAKIYGPLMSLRLGSQCLVVGSSSSTAIEILKTHDRILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P R +L+ IS+GWT +C+ W+ LR LC+++LFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AVPAKRSDLDKISMGWTFECHNEWRYLRTLCRTDLFSGKVLESQAYLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT+ NMLSN+L+S+D+++L+++IE ++NL+RG+VE +SAPN+SD
Sbjct: 166 SKEGQVVNIGELVFATVLNMLSNVLISKDMVNLEKEIEDGGIRNLIRGLVEAISAPNVSD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIH--EANQRDFLDTLIDQKFSD 727
YP+ +LD QGLR +A + ++ S I+EERRKN ++Q DFL+ L+D ++
Sbjct: 226 FYPVLGKLDLQGLRKRARYVMTEIRSNWELILEERRKNKESGSSSQHDFLEALLDNGLTN 285
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++ +EL +AG+DTSTSTI+W MAELIKN E MKK++EEL+IE+ + KES ++
Sbjct: 286 DCIHQLFVELVSAGSDTSTSTIEWAMAELIKNVESMKKVQEELEIELSESDYPKESQLLQ 345
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KET +E C+VMN+TIPK+AQ++VN+WAI RDP++WE+P
Sbjct: 346 MSYVQACVKETLRLHPPAPFLLPHRAIETCQVMNHTIPKDAQILVNIWAITRDPLIWEDP 405
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF++S +DF G DF FLPFGAGRR+CPG PMAA I L++ASLVH FDW LP+G
Sbjct: 406 EMFRPQRFLSSDMDFKGNDFEFLPFGAGRRICPGLPMAAIIIPLLLASLVHFFDWELPHG 465
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
E P LDM E+FG+TLQKK+P++LIP+PRK
Sbjct: 466 EHPTELDMTEKFGVTLQKKEPLLLIPKPRK 495
>XP_015085141.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum pennellii]
Length = 488
Score = 504 bits (1298), Expect = e-172
Identities = 242/450 (53%), Positives = 336/450 (74%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI +GK+PH ++ N A+ YGP+M+ KLG K L+VGSSP A+EIL D ILSGRD P
Sbjct: 39 NIFQMGKMPHITLTNLAKTYGPLMSLKLGTKWLVVGSSPLAAIEILKTHDRILSGRDVPN 98
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P +L+ S+GW +CN W+ LR LC++ELF+GK LESQ LREKK+ ++V+FL+
Sbjct: 99 AAPSKGLDLDKTSLGWRSECNSGWRYLRTLCRTELFAGKVLESQACLREKKVMELVEFLR 158
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
EG+ VNIG++ FAT+ NMLSN+L+S+D+++L+E+IE +K+L+RGIVEV+SAPN+SD
Sbjct: 159 TKEGQVVNIGELVFATVMNMLSNVLISKDMVNLEEEIEDGEIKSLVRGIVEVLSAPNISD 218
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHE--ANQRDFLDTLIDQKFSD 727
+YP+ +LD QGL+ K+ L +L S PI+EERR + + ++Q+DFL+TL+D F +
Sbjct: 219 LYPVLGKLDLQGLQKKSIDLVTKLCSNWEPILEERRNSREKGSSSQQDFLETLLDNGFIN 278
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++LMEL AG+DT+TSTI+W MAELIKN E MKK++EEL+IE+ + +KES ++
Sbjct: 279 DSIHQLLMELLLAGSDTTTSTIEWAMAELIKNVESMKKVQEELEIELSESDYLKESQLLQ 338
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KET ++ C+VM+YTIPK+AQ+ VNVWAI RDP++WE P
Sbjct: 339 MSYIQACVKETLRLHPPGPLLLPHRAIKTCQVMSYTIPKDAQIFVNVWAITRDPLIWEEP 398
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF+NS +DF G DF +LPFGAGRR+CP PMAA I LV+ASLVH FDW LP+G
Sbjct: 399 DMFRPQRFLNSDMDFKGNDFEYLPFGAGRRICPALPMAAIIIPLVLASLVHFFDWELPHG 458
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ P LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 459 KCPVELDMTEQFGVTLQKKEPLLLIPKARK 488
>XP_006358870.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum tuberosum]
Length = 495
Score = 504 bits (1298), Expect = e-172
Identities = 239/450 (53%), Positives = 338/450 (75%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+IYGP+M+ +LG + L+VGSS + A+EIL D ILSGR P
Sbjct: 46 NIFHMGKMPHITLTNFAKIYGPLMSLRLGTQCLVVGSSSSAAIEILKTHDRILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P R +L+ IS+GW +C+ W+ LR LCK+ELFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AMPAKRSDLDKISMGWNSECHNEWRCLRTLCKTELFSGKVLESQAYLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT+ NMLSN+L+S+D+++L+++ E ++NL+RG+VE VSAPNLSD
Sbjct: 166 SKEGQVVNIGELVFATVLNMLSNVLISKDMVNLEKETEDGGIRNLIRGLVEAVSAPNLSD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKN--IHEANQRDFLDTLIDQKFSD 727
YP+ +LD QGLR +A + ++ S PI+EERRKN ++Q+DFL+ L+D ++
Sbjct: 226 FYPVLGKLDLQGLRKRARYVMTKIRSNWEPILEERRKNKVSGSSSQQDFLEALLDNGLTN 285
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++ +EL AG+DTSTSTI+W MAELIKN E MKK++EEL+IE+ + ES ++
Sbjct: 286 DCIHQLFVELVAAGSDTSTSTIEWAMAELIKNVESMKKVQEELEIELSESDYPNESQLLQ 345
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KET +E C+VM+YTIPK+AQ++VNVWAI RDP++WE+P
Sbjct: 346 MSYVQACMKETLRLHPPAPFLLPHRAIETCQVMSYTIPKDAQILVNVWAITRDPLIWEDP 405
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF++S +DF G DF FLPFGAGRR+CPG PMAA ++ LV+ASLVH FDW LP+G
Sbjct: 406 EMFRPQRFLSSDMDFKGNDFEFLPFGAGRRICPGLPMAAIKVPLVLASLVHFFDWELPHG 465
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ P LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 466 KRPTELDMTEKFGVTLQKKEPLLLIPKARK 495
>XP_004245646.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X1 [Solanum lycopersicum]
Length = 496
Score = 503 bits (1294), Expect = e-171
Identities = 241/451 (53%), Positives = 339/451 (75%), Gaps = 3/451 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+IYGP+M+ +LG + L+VGSS + A+EIL D ILSGR P
Sbjct: 46 NIFHMGKMPHITLTNFAKIYGPLMSLRLGSQCLVVGSSTSTAIEILKTHDRILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P R +L+ IS+GWT +C+ W+ LR LC++ELFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AVPAKRSDLDKISMGWTSECHNEWRYLRTLCRAELFSGKVLESQAYLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQ-VAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLS 904
+ EG+ VNIG+ V FAT+ NMLSN+L+S+D+++L+++IE ++NL+RG+VE +SAPN+S
Sbjct: 166 SKEGQVVNIGELVVFATVLNMLSNVLISKDMVNLEKEIEDGGIRNLIRGLVEAISAPNVS 225
Query: 903 DIYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHE--ANQRDFLDTLIDQKFS 730
D YP+ +LD QGLR +A + ++ S I+EERRKN ++Q DFL+ L+D +
Sbjct: 226 DFYPVLGKLDLQGLRKRARYVMTEIRSNWELILEERRKNKESGSSSQHDFLEALLDNGLT 285
Query: 729 DAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIM 550
+ +++ +EL +AG+DTSTSTI+W MAELIKN E MKK++EEL+IE+ + KES ++
Sbjct: 286 NDCIHQLFVELVSAGSDTSTSTIEWAMAELIKNVETMKKVQEELEIELSESDYPKESQLL 345
Query: 549 NLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWEN 370
+ Y+QAC+KET +E C+VMN+TIPK+AQ++VNVWAI RDP++WE+
Sbjct: 346 QMSYVQACVKETLRLHPPAPFLLPHRAIETCQVMNHTIPKDAQILVNVWAIMRDPLIWED 405
Query: 369 PLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPN 190
P F+P+RF++S +DF G DF FLPFGAGRR+CPG PMAA I LV+ASLVH FDW LP+
Sbjct: 406 PEMFRPQRFLSSDMDFKGNDFEFLPFGAGRRICPGLPMAAIIIPLVLASLVHFFDWELPH 465
Query: 189 GEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
E P LDM E+FG+TLQKK+P++LIP+PRK
Sbjct: 466 RERPTELDMTEKFGVTLQKKEPLLLIPKPRK 496
>XP_016502026.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tabacum]
Length = 496
Score = 496 bits (1276), Expect = e-168
Identities = 235/451 (52%), Positives = 334/451 (74%), Gaps = 3/451 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++A +A+ YGP+M+ KLG + L+VGSSP++A+EIL D ILSGR P
Sbjct: 46 NIIHMGKMPHITLAKFAKTYGPLMSLKLGTQCLVVGSSPSVAIEILKTHDRILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
AFP + EL+ SIGWT +CN W+ LR LC++ELFSGK LESQ REKK ++V+FL+
Sbjct: 106 AFPFKKSELDKSSIGWTSECNNGWRYLRTLCRTELFSGKVLESQACSREKKAIELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ +N+G++ FAT+ NMLSN+++S+D++ L+++ E MK+L+R +EV S PN+SD
Sbjct: 166 SKEGQVINLGELIFATVLNMLSNVMISQDIVKLEKETEDGGMKSLIREAMEVGSTPNISD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGP-IVEERRKNIHE--ANQRDFLDTLIDQKFS 730
YPI ++LD QGL K+ L ++ S + IVEERRK+ ++Q+DFL+TL+D F+
Sbjct: 226 FYPILSKLDLQGLHRKSKDLMSKIRSKMWERIVEERRKSKESGFSSQQDFLETLLDNGFT 285
Query: 729 DAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIM 550
+ +++ MELF AG DT+TSTI+W MAELIKN E M+KL++EL+ E+ + KES ++
Sbjct: 286 NDAIHQLFMELFTAGTDTTTSTIEWAMAELIKNEESMEKLQDELERELSESDFPKESKLL 345
Query: 549 NLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWEN 370
+ Y+QAC++ET +E C+VM+YTIPK+AQV+VN+WAIGRDP +W+
Sbjct: 346 QMSYIQACVRETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNIWAIGRDPSIWKE 405
Query: 369 PLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPN 190
P F+P+RF++S +DF G DF FLPFG+GRR+CPG PMAA+ + LV+ASLVH FDW LPN
Sbjct: 406 PEMFRPQRFLSSIMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPN 465
Query: 189 GEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
G+ LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 466 GKCSIELDMNEKFGVTLQKKEPLLLIPKARK 496
>XP_006358914.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum tuberosum]
Length = 496
Score = 496 bits (1276), Expect = e-168
Identities = 237/450 (52%), Positives = 330/450 (73%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI +GK+PH ++ N+A+ YGP+++ +LG + L+VGSSP++A+EI D ILSGR P
Sbjct: 47 NIFQIGKMPHITLTNFAKTYGPLISLRLGTQCLVVGSSPSVAIEIFKTHDRILSGRHVPN 106
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A +L S+GW +C+ W+ LR LC+++LF+GK LESQ LREKK+ ++V+FL+
Sbjct: 107 AVRSKGSDLEKTSLGWKSECDSGWRYLRTLCRTDLFAGKVLESQACLREKKVMELVEFLR 166
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT+ NMLSN+L+S+D+I L+E+ E S +K+L+RGIVEV SAPN+SD
Sbjct: 167 SKEGQVVNIGELVFATVLNMLSNVLISKDMIHLEEETEDSEIKSLVRGIVEVGSAPNISD 226
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERR--KNIHEANQRDFLDTLIDQKFSD 727
YPI +LD QGLR K+ L + SI PI+EERR + ++Q+DFL+TL+D F++
Sbjct: 227 FYPILGKLDLQGLRKKSTDLVTKFCSIWEPIIEERRNSRENDSSSQQDFLETLLDNGFTN 286
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
N++ MEL +AG+DTST+ I+W MAELIKN E MKK++EEL+IE+ + +KES ++
Sbjct: 287 DHINQLFMELLSAGSDTSTTAIEWAMAELIKNVETMKKVQEELEIELSESDYLKESQVLQ 346
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KE +E C+VM+YTIPK AQ++VNVWAI RDP +WE P
Sbjct: 347 MSYVQACVKEAFRLHPPAPLLLPHRAIETCQVMSYTIPKGAQILVNVWAITRDPFIWEEP 406
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF++S +DF G DF FLPF AGRRMCPG PMAA Q+ LV+ASLVH FDW LP+G
Sbjct: 407 DMFRPQRFLSSDMDFKGNDFEFLPFSAGRRMCPGMPMAAIQVPLVLASLVHFFDWELPHG 466
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ LDM E+FGITLQKK+P++LIP+ RK
Sbjct: 467 KCLIELDMTEKFGITLQKKEPLLLIPKARK 496
>XP_019179544.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Ipomoea nil]
Length = 511
Score = 494 bits (1271), Expect = e-167
Identities = 240/453 (52%), Positives = 331/453 (73%), Gaps = 5/453 (1%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI HLGK H ++ +A YGP+++ KLG ++L+VGSSP++A EIL D +LSGR P
Sbjct: 61 NIFHLGKKTHITLTTFANTYGPLISLKLGTQHLVVGSSPSVASEILKTHDRLLSGRHVPH 120
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
AFP +L+H SIGWT +C+E W++LRALC++E+F + LESQ LREKK +MV FL+
Sbjct: 121 AFPTETSQLDHTSIGWTSECHEGWRSLRALCRAEMFCSRALESQALLREKKAGEMVGFLR 180
Query: 1080 ANEGKT--VNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNL 907
+ G V++G++ FAT+ NMLSN++LSRD+I + D MK L+R ++EV SAPNL
Sbjct: 181 SKVGSEEGVDVGEMVFATVLNMLSNVMLSRDVIRFEGD---GVMKGLIRSVMEVASAPNL 237
Query: 906 SDIYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRK---NIHEANQRDFLDTLIDQK 736
SD YP LDPQGLR +A L ++ S+ PI+EERR+ + ++ +DFLDTL+D+
Sbjct: 238 SDFYPFLQRLDPQGLRKRALGLGMRIRSMWEPIIEERREVRCSSGGSSHQDFLDTLLDKG 297
Query: 735 FSDAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESH 556
FS+ +++ MELF AG DTSTSTI+W MAELIK+PE M+K+ +EL E I G I+ESH
Sbjct: 298 FSNERIHQLFMELFAAGTDTSTSTIEWAMAELIKSPESMEKVYKELKRE-INGGDIRESH 356
Query: 555 IMNLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILW 376
+++LPYLQAC+KET +E C+VMNYTIPK+AQ+++NVWA+ RD +W
Sbjct: 357 LVHLPYLQACVKETLRLHPPAPLLLPHRAIEVCQVMNYTIPKDAQILINVWAVQRDASIW 416
Query: 375 ENPLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSL 196
+ PL+F+PERF+ + L+F G DF FLPFGAGRR+CPG PMAA+ + LV+AS++HSFDWSL
Sbjct: 417 DEPLKFRPERFLETGLEFKGNDFEFLPFGAGRRICPGLPMAAKTVPLVLASMIHSFDWSL 476
Query: 195 PNGEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
P+G+ P L+M E+FG+TLQK+QP+ LIP+ RK
Sbjct: 477 PDGKNPKDLNMNEKFGVTLQKEQPLFLIPKTRK 509
>XP_019070768.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X2 [Solanum lycopersicum]
Length = 496
Score = 492 bits (1267), Expect = e-167
Identities = 235/451 (52%), Positives = 335/451 (74%), Gaps = 3/451 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+IYGP+M+ +LG + L+VGSS + A+EIL D ILSGR P
Sbjct: 46 NIFHMGKMPHITLTNFAKIYGPLMSLRLGSQCLVVGSSTSTAIEILKTHDRILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P R +L+ IS+GWT +C+ W+ LR LC++ELFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AVPAKRSDLDKISMGWTSECHNEWRYLRTLCRAELFSGKVLESQAYLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQ-VAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLS 904
+ EG+ VNIG+ V FAT+ NMLSN+L+S+D+++L+++IE ++NL+RG+VE +SAPN+S
Sbjct: 166 SKEGQVVNIGELVVFATVLNMLSNVLISKDMVNLEKEIEDGGIRNLIRGLVEAISAPNVS 225
Query: 903 DIYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHE--ANQRDFLDTLIDQKFS 730
D YP+ +LD QGLR +A + ++ S I+EERRKN ++Q DFL+ L+D +
Sbjct: 226 DFYPVLGKLDLQGLRKRARYVMTEIRSNWELILEERRKNKESGSSSQHDFLEALLDNGLT 285
Query: 729 DAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIM 550
+ +++ +EL AG+DT+TSTI+W MAELIKN E MKK++EEL+IE+ + +KES ++
Sbjct: 286 NDCIHQLFVELLLAGSDTTTSTIEWAMAELIKNVESMKKVQEELEIELSESDYLKESQLL 345
Query: 549 NLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWEN 370
+ Y+QAC+KET ++ C+VM+YTIPK+AQ+ VNVWAI RDP++WE
Sbjct: 346 QMSYIQACVKETLRLHPPGPLLLPHRAIKTCQVMSYTIPKDAQIFVNVWAITRDPLIWEE 405
Query: 369 PLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPN 190
P F+P+RF+NS +DF G DF +LPFGAGRR+CP PMAA +I LV+A LV FDW LP+
Sbjct: 406 PDMFRPQRFLNSDMDFKGNDFEYLPFGAGRRICPALPMAAIEIPLVLALLVRFFDWELPH 465
Query: 189 GEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
G+ P LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 466 GKCPVELDMTEQFGVTLQKKEPLLLIPKARK 496
>XP_016567613.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Capsicum annuum]
Length = 495
Score = 492 bits (1266), Expect = e-167
Identities = 239/450 (53%), Positives = 326/450 (72%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+ YGPI++ K G K L+VGSSP++A+EIL D ILS R P
Sbjct: 46 NIFHMGKMPHITLTNFAKAYGPIISLKFGTKWLVVGSSPSVAIEILKTHDRILSARHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P +L S+GW C+ W+ LR LCK+ELFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AVPSEGSDLEKTSLGWNSGCSSRWRYLRTLCKTELFSGKVLESQACLREKKVKELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT NMLSN+L+S+D+ + + E ++NL+R IVE VSAPN+SD
Sbjct: 166 SKEGQVVNIGELVFATALNMLSNVLISKDMANFDNETEDGDIRNLVREIVEAVSAPNISD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERRKNIH--EANQRDFLDTLIDQKFSD 727
Y I +LD QGLR K ++ +Q S PI+EERR + ++Q+DFL+TL+D F++
Sbjct: 226 FYQILGKLDLQGLRKKYINVAKQFRSKWEPILEERRNSRENGSSSQQDFLETLLDNDFTN 285
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++ MEL +AG+DT TSTI+W MAELIKN E MKK++EEL+IE+ K +KES ++
Sbjct: 286 DCIHQLFMELLSAGSDTITSTIEWAMAELIKNVEPMKKVQEELEIELSKSDYLKESQLLQ 345
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ Y+QAC+KET +E C+VM+YTIPK+AQ+MVNVWAI RDP++WE P
Sbjct: 346 MSYVQACVKETLRLHPPAPLLLPHRAIESCQVMSYTIPKDAQIMVNVWAITRDPLIWEEP 405
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF+ S +DF G DF FLPFGAGRR+CPG PMAA +I LV+ASLVH FDW LP+G
Sbjct: 406 EMFRPQRFLRSDMDFKGNDFEFLPFGAGRRICPGLPMAAIKIPLVLASLVHFFDWELPHG 465
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ P LDM E+ G+TLQKK+P++LIP+ RK
Sbjct: 466 KCPAELDMTEKIGVTLQKKEPLLLIPKARK 495
>XP_016567623.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Capsicum annuum]
Length = 493
Score = 491 bits (1264), Expect = e-167
Identities = 237/453 (52%), Positives = 326/453 (71%), Gaps = 5/453 (1%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++AN+A+ YGP+M+ KLG K L+VGSS A+EIL D ILSGRD P
Sbjct: 41 NIFHMGKMPHITLANFAKTYGPLMSLKLGTKLLVVGSSTCAAVEILKTHDRILSGRDVPN 100
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P R L+ ISIGW + W+ LR +CK+ELFS K LESQ LRE+K+ ++V+FL+
Sbjct: 101 AVPLKRSVLDRISIGWASESKYGWRFLRTVCKNELFSSKVLESQACLREEKVMELVEFLR 160
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT NMLSN+++S+D+ + + E ++NL+RG+VE +S PN+SD
Sbjct: 161 SKEGQVVNIGELVFATALNMLSNVMISKDMANFDNETEDGEIRNLVRGLVEAISTPNISD 220
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERR-----KNIHEANQRDFLDTLIDQK 736
YPI +LD QGLR KA + ++ S PI+EERR K ++Q+DFL+ L+D
Sbjct: 221 FYPILGKLDLQGLREKARYVMAEIRSNWEPILEERRQRRKNKESGSSSQQDFLEALLDNG 280
Query: 735 FSDAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESH 556
+ +++ +EL +AG+DT TSTI+W MAELIKN E MKK++EEL+IE+ K +KES
Sbjct: 281 VPNDCIHQLFVELLSAGSDTITSTIEWAMAELIKNVEPMKKVQEELEIELSKSDYLKESQ 340
Query: 555 IMNLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILW 376
++ + Y+QAC+KET +E C+VM+YTIPK+AQ+MVNVWAI RDP++W
Sbjct: 341 LLQMSYVQACVKETLRLHPPAPLLLPHRAIESCQVMSYTIPKDAQIMVNVWAITRDPLIW 400
Query: 375 ENPLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSL 196
E P F+P+RF+ S +DF G DF FLPFGAGRR+CPG PMAA ++ LV+ASLVH FDW L
Sbjct: 401 EEPEMFRPQRFLRSDMDFKGNDFEFLPFGAGRRICPGLPMAAIKVPLVLASLVHFFDWEL 460
Query: 195 PNGEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
P+G+ P LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 461 PHGKCPAELDMTEKFGVTLQKKEPLLLIPKARK 493
>XP_016567604.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Capsicum annuum]
Length = 494
Score = 488 bits (1255), Expect = e-165
Identities = 236/450 (52%), Positives = 330/450 (73%), Gaps = 2/450 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ N+A+ YGP+M +LG + L+VGSSP++A+EIL D ILS R P
Sbjct: 46 NIFHMGKMPHITLTNFAKTYGPLMCLRLGTQWLVVGSSPSVAIEILKTHDRILSARHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
A P +L S+GW C+ W+ LR LCK+ELFSGK LESQ LREKK+ ++V+FL+
Sbjct: 106 AVPSKGSDLEKTSLGWNSGCSSRWRYLRTLCKTELFSGKVLESQACLREKKVMELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ VNIG++ FAT+ NMLSN+L+S+D+++L ++ E +K+L+R IVEV SAPN+SD
Sbjct: 166 SKEGQVVNIGELVFATVLNMLSNVLISKDMVNLDKETEDGGIKSLVREIVEVASAPNISD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERR--KNIHEANQRDFLDTLIDQKFSD 727
YPI +LD QGLR K+ L + S PI+EERR + ++Q+DFL+TL+D F++
Sbjct: 226 FYPILGKLDLQGLRKKSIRLVIKFCSKWEPILEERRDSRENGSSSQQDFLETLLDNGFTN 285
Query: 726 AGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMN 547
+++ MEL +AG+DTSTSTI+W MAELIK+ E MKK++EEL+IE + +KES ++N
Sbjct: 286 DRIHQLFMELLSAGSDTSTSTIEWAMAELIKDVESMKKVQEELEIE-SESDYLKESQVLN 344
Query: 546 LPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENP 367
+ ++QAC+KET +E C++M+YTIPK+AQ+MVNVWAI RDP +WE P
Sbjct: 345 MSFVQACVKETLRLHPPVPFLLPHRAIETCQIMSYTIPKDAQIMVNVWAIARDPSIWEEP 404
Query: 366 LEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNG 187
F+P+RF++S +DF G DF FLPFGAGRR+CPG PMAA ++ LV+ASLVH FDW LP+G
Sbjct: 405 EMFRPQRFLSSDMDFKGNDFEFLPFGAGRRICPGLPMAAIKVLLVVASLVHYFDWELPHG 464
Query: 186 EGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+ LDM E+FG+ LQKK+P++LIP+ RK
Sbjct: 465 KCAAELDMIEKFGVALQKKEPLLLIPKARK 494
>XP_009609252.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tomentosiformis]
Length = 495
Score = 485 bits (1248), Expect = e-164
Identities = 232/448 (51%), Positives = 329/448 (73%), Gaps = 3/448 (0%)
Frame = -3
Query: 1431 HLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPRAFP 1252
H+GK+PH ++ N+A+ YGP+M+ KLG + L+VGSSP++A+EIL D ILSGR P AFP
Sbjct: 48 HMGKMPHITLTNFAKTYGPLMSLKLGTQCLVVGSSPSVAIEILKTHDRILSGRHVPNAFP 107
Query: 1251 HSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLKANE 1072
+ EL SIGWT +CN W+ LR LC++ELFSGK LESQ LREKK+ ++++FL++ E
Sbjct: 108 FKKSELEKSSIGWTSECNNGWRYLRTLCRTELFSGKVLESQACLREKKVKELIEFLRSKE 167
Query: 1071 GKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSDIYP 892
G+ VN+G++ FAT+ NMLSN+++S+D++ L+++ E MK+L+R +EV S PN+SD YP
Sbjct: 168 GQVVNLGELIFATVLNMLSNVMISQDIVKLEKETEDGGMKSLIREAMEVGSTPNISDFYP 227
Query: 891 IFNELDPQGLRNKANSLWQQLWS-ILGPIVEERRKNIH--EANQRDFLDTLIDQKFSDAG 721
I +LD QGLR K+ L ++ S IVEERRK+ ++Q DFL+TL++ F++
Sbjct: 228 ILCKLDLQGLRRKSMVLMSKIRSKTWERIVEERRKSKESGSSSQHDFLETLLNNGFTNDA 287
Query: 720 FNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMNLP 541
+++ MELF AG DT+TSTI+W +AELIKN E M+K+R EL+ E+ + KES ++ +
Sbjct: 288 IHQLFMELFTAGTDTTTSTIEWAIAELIKNEESMEKVRAELERELRESYFPKESKLLQMS 347
Query: 540 YLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENPLE 361
Y+QAC+KET +E C+VM+YTIPK+AQV+VNVWAIGRD ++W+ P
Sbjct: 348 YIQACVKETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNVWAIGRDSLIWKEPEM 407
Query: 360 FKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNGEG 181
F+P+RF++S +DF G DF FLPFG+GRR+CPG PMAA+ + LV+ASLVH FDW LPNG+
Sbjct: 408 FRPQRFLSSNMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPNGKC 467
Query: 180 PDMLDMKEEFGITLQKKQPMILIPRPRK 97
LDM E+FG+TLQK++P++LIP+ K
Sbjct: 468 SIELDMNEKFGVTLQKREPLLLIPKATK 495
>XP_019238293.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana attenuata]
Length = 496
Score = 485 bits (1248), Expect = e-164
Identities = 233/451 (51%), Positives = 328/451 (72%), Gaps = 3/451 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI H+GK+PH ++ +A+ YGP+M+ KLG K L+VGSSP+ A+EIL D ILSGR P
Sbjct: 46 NILHMGKMPHITLTTFAKTYGPLMSLKLGTKCLVVGSSPSAAIEILKTHDKILSGRHVPN 105
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
AFP + EL SIGWT +CN W+ LR LC++ELFSGK LESQ RE+K ++V+FL+
Sbjct: 106 AFPFKKSELEKSSIGWTSECNNEWRYLRTLCRTELFSGKVLESQACSREEKAIELVEFLR 165
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG+ +N+G++ FAT+ NMLSN+++S+D++ L+++IE K+L+R +EV SAPN+SD
Sbjct: 166 SKEGQVINLGELIFATVLNMLSNVMISQDIVKLEKEIEDGGTKSLIREAMEVGSAPNISD 225
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWS-ILGPIVEERRKN--IHEANQRDFLDTLIDQKFS 730
YP ++LD QGLR K+ L ++ S IV+ERRK+ +Q+DFL+TL++ F+
Sbjct: 226 FYPFLSKLDLQGLRRKSMVLMSKIRSKTWERIVKERRKSKETGSTSQQDFLETLLNNGFT 285
Query: 729 DAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIM 550
+ +++ MELF AG DT+TSTI+W MAELIK E M+KL++EL E+I+ ES ++
Sbjct: 286 NDAIHQLFMELFTAGTDTTTSTIEWAMAELIKYEESMEKLQDELGRELIESDFPNESKLL 345
Query: 549 NLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWEN 370
+ Y+QAC+KET +E C+VM+YTIPK+AQV+VN+WAIGRDP +W+
Sbjct: 346 QMSYIQACVKETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNIWAIGRDPTIWKE 405
Query: 369 PLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPN 190
P F+P+RF++S +DF G DF FLPFG+GRR+CPG PMAA+ + LV+ASLVH FDW LPN
Sbjct: 406 PEMFRPQRFLSSNMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPN 465
Query: 189 GEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
G+ LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 466 GKCSIDLDMNEKFGVTLQKKEPLLLIPKARK 496
>XP_016469425.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tabacum]
Length = 447
Score = 480 bits (1236), Expect = e-163
Identities = 231/447 (51%), Positives = 328/447 (73%), Gaps = 3/447 (0%)
Frame = -3
Query: 1428 LGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPRAFPH 1249
+GK+PH ++ N+A+ YGP+M+ KLG + L+VGSSP+ A+EIL D ILSGR P AFP
Sbjct: 1 MGKMPHITLTNFAKTYGPLMSLKLGTQCLVVGSSPSAAIEILKTHDRILSGRHVPNAFPF 60
Query: 1248 SRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLKANEG 1069
+ EL SIGWT +CN W+ LR LC++ELFSGK LESQ LREKK+ ++++FL++ EG
Sbjct: 61 KKSELEKSSIGWTSECNNGWRYLRTLCRTELFSGKVLESQACLREKKVKELIEFLRSKEG 120
Query: 1068 KTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSDIYPI 889
+ VN+G++ FAT+ NMLSN+++S+D++ L+++ E MK+L+R +EV S PN+SD YPI
Sbjct: 121 QLVNLGELIFATVLNMLSNVMISQDIVKLEKETEDGGMKSLIREAMEVGSTPNISDFYPI 180
Query: 888 FNELDPQGLRNKANSLWQQLWS-ILGPIVEERRKN--IHEANQRDFLDTLIDQKFSDAGF 718
+LD QGLR K+ L ++ S IVEERRK+ ++Q DFL+TL++ F++
Sbjct: 181 LCKLDLQGLRRKSMVLMSKIRSKTWERIVEERRKSKVSGSSSQHDFLETLLNNGFTNDAI 240
Query: 717 NEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMNLPY 538
+++ MELF AG DT+TSTI+W +AELIKN E M+K++ EL+ E+ + KES ++ + Y
Sbjct: 241 HQLFMELFTAGTDTTTSTIEWAIAELIKNEESMEKVQAELERELRESYFPKESKLLQMSY 300
Query: 537 LQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENPLEF 358
+QAC+KET +E C+VM+YTIPK+AQV+VNVWAIGRD ++W+ P F
Sbjct: 301 IQACVKETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNVWAIGRDSLIWKEPEMF 360
Query: 357 KPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNGEGP 178
+P+RF++S +DF G DF FLPFG+GRR+CPG PMAA+ + LV+ASLVH FDW LPNG+
Sbjct: 361 RPQRFLSSNMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPNGKCS 420
Query: 177 DMLDMKEEFGITLQKKQPMILIPRPRK 97
LDM E+FG+TLQK++P++LIP+ RK
Sbjct: 421 IELDMNEKFGVTLQKREPLLLIPKARK 447
>OIT21830.1 cytochrome p450 76c2 [Nicotiana attenuata]
Length = 447
Score = 479 bits (1233), Expect = e-163
Identities = 230/447 (51%), Positives = 325/447 (72%), Gaps = 3/447 (0%)
Frame = -3
Query: 1428 LGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPRAFPH 1249
+GK+PH ++ +A+ YGP+M+ KLG K L+VGSSP+ A+EIL D ILSGR P AFP
Sbjct: 1 MGKMPHITLTTFAKTYGPLMSLKLGTKCLVVGSSPSAAIEILKTHDKILSGRHVPNAFPF 60
Query: 1248 SRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLKANEG 1069
+ EL SIGWT +CN W+ LR LC++ELFSGK LESQ RE+K ++V+FL++ EG
Sbjct: 61 KKSELEKSSIGWTSECNNEWRYLRTLCRTELFSGKVLESQACSREEKAIELVEFLRSKEG 120
Query: 1068 KTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSDIYPI 889
+ +N+G++ FAT+ NMLSN+++S+D++ L+++IE K+L+R +EV SAPN+SD YP
Sbjct: 121 QVINLGELIFATVLNMLSNVMISQDIVKLEKEIEDGGTKSLIREAMEVGSAPNISDFYPF 180
Query: 888 FNELDPQGLRNKANSLWQQLWS-ILGPIVEERRKN--IHEANQRDFLDTLIDQKFSDAGF 718
++LD QGLR K+ L ++ S IV+ERRK+ +Q+DFL+TL++ F++
Sbjct: 181 LSKLDLQGLRRKSMVLMSKIRSKTWERIVKERRKSKETGSTSQQDFLETLLNNGFTNDAI 240
Query: 717 NEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMNLPY 538
+++ MELF AG DT+TSTI+W MAELIK E M+KL++EL E+I+ ES ++ + Y
Sbjct: 241 HQLFMELFTAGTDTTTSTIEWAMAELIKYEESMEKLQDELGRELIESDFPNESKLLQMSY 300
Query: 537 LQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENPLEF 358
+QAC+KET +E C+VM+YTIPK+AQV+VN+WAIGRDP +W+ P F
Sbjct: 301 IQACVKETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNIWAIGRDPTIWKEPEMF 360
Query: 357 KPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNGEGP 178
+P+RF++S +DF G DF FLPFG+GRR+CPG PMAA+ + LV+ASLVH FDW LPNG+
Sbjct: 361 RPQRFLSSNMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPNGKCS 420
Query: 177 DMLDMKEEFGITLQKKQPMILIPRPRK 97
LDM E+FG+TLQKK+P++LIP+ RK
Sbjct: 421 IDLDMNEKFGVTLQKKEPLLLIPKARK 447
>XP_010325430.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X4 [Solanum lycopersicum]
Length = 439
Score = 478 bits (1229), Expect = e-162
Identities = 230/442 (52%), Positives = 323/442 (73%)
Frame = -3
Query: 1422 KLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPRAFPHSR 1243
K+PH ++ N A+ YGP+M+ KLG K L+VGSS A+EIL D ILSGRD P A P
Sbjct: 3 KMPHITLTNLAKTYGPVMSLKLGTKWLVVGSSSLAAIEILKTHDRILSGRDVPNAAPSKG 62
Query: 1242 QELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLKANEGKT 1063
+L+ S+GW +CN W+ LR LC++ELF+GK LESQ LREKK+ ++V+FL++ EG+
Sbjct: 63 LDLDKTSLGWRSECNSGWRYLRTLCRAELFAGKVLESQACLREKKVMELVEFLRSKEGQV 122
Query: 1062 VNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSDIYPIFN 883
VNIG++ FAT+ NML N+L+S+D+++L+E+IE +K+L+RGIVEV+SAPN+SD+YP+
Sbjct: 123 VNIGELVFATVMNMLGNVLISKDMVNLEEEIEDGEIKSLVRGIVEVLSAPNISDLYPVLG 182
Query: 882 ELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHEANQRDFLDTLIDQKFSDAGFNEILM 703
+LD QGL+ K+ L +L S PI+EE+R ++R L+D F++ +++LM
Sbjct: 183 KLDLQGLQKKSIDLVTKLCSNWEPILEEKRN-----SRRTRFLGLLDNGFTNDSIHQLLM 237
Query: 702 ELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMNLPYLQACI 523
EL AG+DT+TSTI+W MAELIKN E MKK++EEL+IE+ + +KES ++ + Y+QAC+
Sbjct: 238 ELLLAGSDTTTSTIEWAMAELIKNVESMKKVQEELEIELSESDYLKESQLLQMSYIQACV 297
Query: 522 KETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENPLEFKPERF 343
KET ++ C+VM+YTIPK+AQ+ VNVWAI RDP++WE P F+P+RF
Sbjct: 298 KETLRLHPPGPLLLPHRAIKTCQVMSYTIPKDAQIFVNVWAITRDPLIWEEPDMFRPQRF 357
Query: 342 VNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNGEGPDMLDM 163
+NS +DF G DF +LPFGAGRR+CP PMAA +I LV+A LV FDW LP+G+ P LDM
Sbjct: 358 LNSDMDFKGNDFEYLPFGAGRRICPALPMAAIEIPLVLALLVRFFDWELPHGKCPVELDM 417
Query: 162 KEEFGITLQKKQPMILIPRPRK 97
E+FG+TLQKK+P++LIP+ RK
Sbjct: 418 TEQFGVTLQKKEPLLLIPKARK 439
>XP_004245647.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X3 [Solanum lycopersicum]
Length = 481
Score = 478 bits (1229), Expect = e-161
Identities = 230/442 (52%), Positives = 323/442 (73%)
Frame = -3
Query: 1422 KLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPRAFPHSR 1243
K+PH ++ N A+ YGP+M+ KLG K L+VGSS A+EIL D ILSGRD P A P
Sbjct: 45 KMPHITLTNLAKTYGPVMSLKLGTKWLVVGSSSLAAIEILKTHDRILSGRDVPNAAPSKG 104
Query: 1242 QELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLKANEGKT 1063
+L+ S+GW +CN W+ LR LC++ELF+GK LESQ LREKK+ ++V+FL++ EG+
Sbjct: 105 LDLDKTSLGWRSECNSGWRYLRTLCRAELFAGKVLESQACLREKKVMELVEFLRSKEGQV 164
Query: 1062 VNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSDIYPIFN 883
VNIG++ FAT+ NML N+L+S+D+++L+E+IE +K+L+RGIVEV+SAPN+SD+YP+
Sbjct: 165 VNIGELVFATVMNMLGNVLISKDMVNLEEEIEDGEIKSLVRGIVEVLSAPNISDLYPVLG 224
Query: 882 ELDPQGLRNKANSLWQQLWSILGPIVEERRKNIHEANQRDFLDTLIDQKFSDAGFNEILM 703
+LD QGL+ K+ L +L S PI+EE+R ++R L+D F++ +++LM
Sbjct: 225 KLDLQGLQKKSIDLVTKLCSNWEPILEEKRN-----SRRTRFLGLLDNGFTNDSIHQLLM 279
Query: 702 ELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIMNLPYLQACI 523
EL AG+DT+TSTI+W MAELIKN E MKK++EEL+IE+ + +KES ++ + Y+QAC+
Sbjct: 280 ELLLAGSDTTTSTIEWAMAELIKNVESMKKVQEELEIELSESDYLKESQLLQMSYIQACV 339
Query: 522 KETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWENPLEFKPERF 343
KET ++ C+VM+YTIPK+AQ+ VNVWAI RDP++WE P F+P+RF
Sbjct: 340 KETLRLHPPGPLLLPHRAIKTCQVMSYTIPKDAQIFVNVWAITRDPLIWEEPDMFRPQRF 399
Query: 342 VNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPNGEGPDMLDM 163
+NS +DF G DF +LPFGAGRR+CP PMAA +I LV+A LV FDW LP+G+ P LDM
Sbjct: 400 LNSDMDFKGNDFEYLPFGAGRRICPALPMAAIEIPLVLALLVRFFDWELPHGKCPVELDM 459
Query: 162 KEEFGITLQKKQPMILIPRPRK 97
E+FG+TLQKK+P++LIP+ RK
Sbjct: 460 TEQFGVTLQKKEPLLLIPKARK 481
>XP_010053332.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Eucalyptus grandis]
Length = 512
Score = 469 bits (1207), Expect = e-158
Identities = 233/452 (51%), Positives = 314/452 (69%), Gaps = 4/452 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
N LGK+PH +++ +A++YGP+++ KLG + +IVGSSP +A EIL ++D +LS R P
Sbjct: 62 NFMQLGKMPHVTLSEFAKVYGPLISLKLGTQLVIVGSSPAVAEEILKEKDHLLSARYVPH 121
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
P R +L++IS+GW+ C WK LR +CK+ELFS +ESQ +REKK+ +MV+ +
Sbjct: 122 VMPVERSKLSYISLGWSPKCGIGWKYLRTICKTELFSRSAIESQTHVREKKVMEMVELIC 181
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
+ EG V+IG+V FAT+ NMLSNIL+S+D I + MKNL+R ++EV + PN+SD
Sbjct: 182 SKEGDVVDIGEVVFATVLNMLSNILVSKDFIEFKNGTVDGEMKNLVRQMMEVSAMPNISD 241
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEERR----KNIHEANQRDFLDTLIDQKF 733
+YPI + LD QG++ K+ L ++ + I+EERR K EA Q DFLD LI+ F
Sbjct: 242 LYPILSGLDLQGIQRKSKELTVKMNDMWTAIIEERRHRKAKEGDEAAQSDFLDVLIENDF 301
Query: 732 SDAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHI 553
+D N++ MELF AG D+S++T++W MAELIKNPE M K+REEL E I Q K KES++
Sbjct: 302 TDEIINQLYMELFTAGTDSSSTTVEWAMAELIKNPESMDKVREELTKE-ISQDKPKESNL 360
Query: 552 MNLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWE 373
L Y++AC+KET C +MNY IPKN+QV+VN+WAIGRDP +W+
Sbjct: 361 FKLQYMEACVKETLRLHPPAPLLLPHRAPRTCSLMNYFIPKNSQVLVNIWAIGRDPTIWK 420
Query: 372 NPLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLP 193
+PLEF PERFV S LDF GK F FLPFGAGRR+CPG PMAA+Q+ LV+ASLV FDW+LP
Sbjct: 421 DPLEFNPERFVQSSLDFKGKHFEFLPFGAGRRICPGLPMAAKQVPLVLASLVKFFDWTLP 480
Query: 192 NGEGPDMLDMKEEFGITLQKKQPMILIPRPRK 97
+G LDM E+ GITLQK QP+ LIP+ RK
Sbjct: 481 HGAELSKLDMTEKLGITLQKAQPLFLIPKVRK 512
>XP_015896285.1 PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Ziziphus jujuba]
Length = 506
Score = 465 bits (1196), Expect = e-156
Identities = 228/447 (51%), Positives = 312/447 (69%), Gaps = 3/447 (0%)
Frame = -3
Query: 1440 NIPHLGKLPHSSVANYAQIYGPIMTFKLGFKNLIVGSSPTIAMEILSKQDLILSGRDTPR 1261
NI +GK PH ++ + AQ YGP+++ + G ++LIVGSSPT AMEIL QD LSGR P
Sbjct: 57 NILQIGKDPHLNLTSLAQTYGPLISLRFGNQHLIVGSSPTAAMEILKTQDRNLSGRYVPH 116
Query: 1260 AFPHSRQELNHISIGWTEDCNESWKNLRALCKSELFSGKTLESQESLREKKIFDMVDFLK 1081
P ELN S+GWT +CNE W+NLR LC+ ELFS K + +Q +REKK+ +M++F+
Sbjct: 117 VAPAKSLELNKFSMGWTSECNEYWRNLRTLCRGELFSTKAISTQACIREKKVMEMLEFIG 176
Query: 1080 ANEGKTVNIGQVAFATIFNMLSNILLSRDLISLQEDIERSTMKNLLRGIVEVVSAPNLSD 901
EGK V I + A +FNMLSN+++SRDLI+L+ + + L+R ++EV S PN+SD
Sbjct: 177 KMEGKEVKIRDLVSAVVFNMLSNVMVSRDLINLENESVNGEISRLVRSMMEVASTPNISD 236
Query: 900 IYPIFNELDPQGLRNKANSLWQQLWSILGPIVEER---RKNIHEANQRDFLDTLIDQKFS 730
I+PI LD QGLR K+ L+ + I I++ER RK+ + + Q+DFLD L+D+
Sbjct: 237 IFPILGPLDLQGLRKKSLELFDRSRKIWEAIIKERQEMRKSSNASTQQDFLDALLDENCP 296
Query: 729 DAGFNEILMELFNAGADTSTSTIDWMMAELIKNPEIMKKLREELDIEVIKQGKIKESHIM 550
+ N + +EL AG DTS+STI+W +AELI+NP MK +++EL E I+Q +KES +
Sbjct: 297 EEQVNMLFLELLTAGTDTSSSTIEWTLAELIRNPRCMKSVQDELARE-IRQDLVKESDLT 355
Query: 549 NLPYLQACIKETXXXXXXXXXXXXXXXLEDCKVMNYTIPKNAQVMVNVWAIGRDPILWEN 370
NL YLQACIKE +E C+VMNYTIPK++ V+VN WAIGRD WE+
Sbjct: 356 NLTYLQACIKEAMRLHPPAPLLLPHRAVESCQVMNYTIPKDSMVLVNFWAIGRDSDHWEH 415
Query: 369 PLEFKPERFVNSPLDFIGKDFIFLPFGAGRRMCPGYPMAARQISLVIASLVHSFDWSLPN 190
PLEFKPERF+NS LDF G DF +PFGAGRR+CPG PMAA+ ++LV+ASL+HSFDWSLP+
Sbjct: 416 PLEFKPERFLNSSLDFKGNDFQLIPFGAGRRICPGLPMAAKHVALVVASLIHSFDWSLPH 475
Query: 189 GEGPDMLDMKEEFGITLQKKQPMILIP 109
G+ P+ LDM E++ +T+ K+QP++LIP
Sbjct: 476 GQDPNNLDMAEKYDLTMMKEQPLLLIP 502