BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00018776
         (1874 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_011090977.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   327   e-106
XP_019227254.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   315   e-103
XP_017649963.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   316   e-103
XP_015064757.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   314   e-103
XP_012456061.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   315   e-103
XP_016677400.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   313   e-102
XP_018825732.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   306   e-102
XP_009612674.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   308   e-102
XP_018629573.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   308   e-102
XP_016542482.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   310   e-101
XP_016542489.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   310   e-101
XP_014510318.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   301   e-101
XP_004229242.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   307   e-101
CDO98863.1 unnamed protein product [Coffea canephora]                 304   e-101
XP_009612677.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   306   e-101
XP_010039753.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   308   e-100
XP_019196477.1 PREDICTED: putative ubiquitin-like-specific prote...   301   e-100
XP_009598750.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   305   e-100
XP_009773778.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   304   e-100
XP_006349816.1 PREDICTED: ubiquitin-like-specific protease ESD4 ...   301   e-100

>XP_011090977.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Sesamum indicum]
          Length = 523

 Score =  327 bits (839), Expect(2) = e-106
 Identities = 204/461 (44%), Positives = 269/461 (58%), Gaps = 22/461 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLS---NFDHISKKRRVSVS------SDNTSTRSRCSVY 1363
            MG L +NR R + +++  +    S   ++ HISKK ++SV        +N  T S   V 
Sbjct: 1    MGALTNNRKRGDDYYKSLVSFSPSIDHSYSHISKKPKLSVPMSEKTPENNRPTSSISVVS 60

Query: 1362 RFNLYPEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYA 1183
            R + YP  K    R VHAPV+N+RF     GLF S K            NT     SK +
Sbjct: 61   RISQYPAGKSGFLREVHAPVRNSRF-----GLFSSAK------------NTESTASSKES 103

Query: 1182 KISREALGVIRHGVFRDVEDEVRVGVPNWR----DKEKGKEVIVLDD-EATEEDVTGGSS 1018
                      R G F    +   V + + R     K+K +EVI +D  +   +DV   SS
Sbjct: 104  PSDNMG----RFGFFHRYNEARDVAIRSLRYTSIGKQKEQEVIEVDSGDENRDDVYDDSS 159

Query: 1017 IEEVDMRIEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVIN----MEDLSL 850
            IEEV++   ++  +   H     + +  N   G++G+G+  +S+ + V N    +ED+  
Sbjct: 160  IEEVEIANFEQSKLKGEHGVGVESSLELNIKLGEKGLGSLDSSVITDVSNATAKVEDVEK 219

Query: 849  KGL-PLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEK---RLEMSWSRXXXXX 682
             GL  L   P+    P +  L D   ++   L  + F I+L+ K   RL++  S+     
Sbjct: 220  MGLMQLDQVPDGPGLPKYKRLYDEIDRKYSKLPVIKFSIELYIKFLQRLQLFRSQKKEDQ 279

Query: 681  XXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLN 502
                   E F+PL+ E+  E++RA + +N RRK+LV+HE SNIDITGEILQCLRPGAWLN
Sbjct: 280  IKKNVSEECFVPLTDEEEREVSRALSNSN-RRKVLVTHENSNIDITGEILQCLRPGAWLN 338

Query: 501  NEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYC 322
            +EVINVYLELLKERE REP KFLKCHF +TFFYKKLI G GGYNFQ+VRRWTTQRKLGY 
Sbjct: 339  DEVINVYLELLKEREKREPQKFLKCHFFSTFFYKKLISGRGGYNFQSVRRWTTQRKLGYS 398

Query: 321  LFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            L +CDKIFVPIHKEVHWCLA+INKKD+KFQYLDSL G D +
Sbjct: 399  LIECDKIFVPIHKEVHWCLAIINKKDEKFQYLDSLKGGDTR 439



 Score = 88.6 bits (218), Expect(2) = e-106
 Identities = 40/57 (70%), Positives = 46/57 (80%)
 Frame = -2

Query: 172 YYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           Y+VDEV +K G  I+VSSW+ EFV DLPEQ NG+DCG+FMIKYADFYSR  GL F Q
Sbjct: 447 YFVDEVKDKCGKDINVSSWEQEFVTDLPEQANGFDCGMFMIKYADFYSRDIGLCFSQ 503


>XP_019227254.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Nicotiana
            attenuata] OIT31517.1 ubiquitin-like-specific protease
            esd4 [Nicotiana attenuata]
          Length = 503

 Score =  315 bits (806), Expect(2) = e-103
 Identities = 199/457 (43%), Positives = 259/457 (56%), Gaps = 20/457 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSN---FDHISKKRRVSVSSDNTSTRSRCSVYRFNLYP 1345
            MG L  NR R    F  +     SN   FDHISKK ++S+    T    + +V RF  YP
Sbjct: 1    MGALTLNRKRGNDIFSSNYKTQFSNPSNFDHISKKPKLSIYQ--TLNPPKSTVQRFFKYP 58

Query: 1344 EAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKISREA 1165
            +    + R VHAP + +RF             +SA +M N +S  +D  K K A  S   
Sbjct: 59   DPINPIRRQVHAPCRQSRFGSK------GNSANSAPEMGNFVSRVYDEAK-KSAFYSLRC 111

Query: 1164 LGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGG-SSIEEVDMRIEK 988
            L  ++  +F D                        DD+A + D+  G     +V   +E 
Sbjct: 112  LKKVKEVIFID------------------------DDDAKKGDLEAGVCKKSDVFCNLEG 147

Query: 987  ---ERPVFYGHQWNTSTGIVE--NAVDGKRGV--------GNCKASMSSPVINMEDLSLK 847
               E+  F G +W  + G++E  + VDGK           GN K   +  ++  + L+L 
Sbjct: 148  GVCEKSEFLGQKWKETNGLLEILDGVDGKDSSVVTTGLDDGNLKEESAGKML--DTLALN 205

Query: 846  GLPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE---MSWSRXXXXXXX 676
              P     + +  P +  LL S  K +  L+ L FQI  +EK LE   +   +       
Sbjct: 206  PTP----DSAFYVPLYKKLLGSVGKINDKLKRLQFQIGYNEKCLETYSLLRPQKKGEQVK 261

Query: 675  XXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNE 496
               + E F+PL+ ED +E++RA +K+N RR++LV+H+ SNIDITGEILQCLRP  WLN+E
Sbjct: 262  EDVISEPFVPLTEEDEAEVSRALSKSN-RRRVLVTHKNSNIDITGEILQCLRPREWLNDE 320

Query: 495  VINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLF 316
            VINVYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGYCL 
Sbjct: 321  VINVYLELLKEREKREPKKFLKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLL 380

Query: 315  DCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            +CDKIFVPIHKE+HWCLAVINKKD+KFQYLDSL GRD
Sbjct: 381  ECDKIFVPIHKEIHWCLAVINKKDKKFQYLDSLRGRD 417



 Score = 92.8 bits (229), Expect(2) = e-103
 Identities = 43/59 (72%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NG+DCGVFMIK ADFYSR  GL F Q
Sbjct: 425 ARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVFMIKNADFYSRDIGLCFNQ 483


>XP_017649963.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Gossypium arboreum]
            KHG04279.1 Ubiquitin-like-specific protease ESD4
            [Gossypium arboreum]
          Length = 495

 Score =  316 bits (810), Expect(2) = e-103
 Identities = 206/459 (44%), Positives = 273/459 (59%), Gaps = 22/459 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDH-------------ISKKRRVSV---SSDNTST 1384
            MG L SNR RY     G  C + SNF H             +SKKRR+S+   S +  + 
Sbjct: 1    MGALASNRKRY-----GDECFN-SNFCHKKKSPYSNSPEFQLSKKRRLSLMNESPEKANL 54

Query: 1383 RSRCSVYRFNLYPEAKGELPRAVHAPVKNNRF--SFSKRGLFGSGKVSSADKMLNSLSNT 1210
             S  +V R + YPE+K   PR VHAPV+  +F  S SK G          D + + + N 
Sbjct: 55   SSNSTVLRISRYPESKFSFPREVHAPVRRLKFWLSASKPG---------QDSVESYMGN- 104

Query: 1209 FDILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVT 1030
               L S+ +   R+A G +RH  F+  E EV + V +  +KEKG   +V DD + EE   
Sbjct: 105  --FLSSRLSDAKRQAWGALRH--FKK-EKEV-IFVEDDEEKEKG---LVSDDVSVEEV-- 153

Query: 1029 GGSSIEEVDMRIEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSL 850
                IE+    +++ER      Q ++S+ + E               +++  + ME  SL
Sbjct: 154  --ELIEKGKNNVKEERHF----QPSSSSAVTE---------------LNNGSLRMES-SL 191

Query: 849  KGLPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE----MSWSRXXXXX 682
              L LR   N +    +  L++SA +R   L++L FQI+L+EKR+     +   +     
Sbjct: 192  DMLSLREVSNGYDLEAYRKLIESAERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEE 251

Query: 681  XXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLN 502
                   E FIPL+ E+ + ++RA + A + RK+LVSHE S+IDI GEILQCL+PGAWLN
Sbjct: 252  QHEVLPTEPFIPLTEEEMALVSRALS-AKNWRKVLVSHENSSIDIRGEILQCLKPGAWLN 310

Query: 501  NEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYC 322
            +EVIN+YLELLKERENREP KFLKCHF NTFFYKKL+    GYN++AV+RWT+QRKLGYC
Sbjct: 311  DEVINLYLELLKERENREPKKFLKCHFFNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYC 370

Query: 321  LFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            L DCDKIFVPIHK++HWCLAVINKKDQKFQYLDSL GRD
Sbjct: 371  LLDCDKIFVPIHKDIHWCLAVINKKDQKFQYLDSLKGRD 409



 Score = 90.9 bits (224), Expect(2) = e-103
 Identities = 39/59 (66%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+V+EV +K+G  ID+SSW+ EFVEDLP QENG+DCG+FM+KY DFYSRG  L F+Q
Sbjct: 417 AKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCGMFMLKYIDFYSRGLSLCFEQ 475


>XP_015064757.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum pennellii]
          Length = 515

 Score =  314 bits (804), Expect(2) = e-103
 Identities = 205/468 (43%), Positives = 261/468 (55%), Gaps = 29/468 (6%)
 Frame = -3

Query: 1515 MGVLI-SNRNRYEAHFRGSLCLDLS---NFDHISKKRRVSVSSDNTSTR--SRCSVY-RF 1357
            MG L  S+R R +  F  +    LS   N DHISKK R+S S +       SR S+  R 
Sbjct: 1    MGALTCSSRKRGDDFFNSNYKTPLSVSSNVDHISKKPRLSPSMNQIQDHPFSRKSIAARI 60

Query: 1356 NLYPEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKI 1177
              YP     + R +HAP +  R           G    +DKM N L+  +D  K      
Sbjct: 61   FKYPSNITPIKREIHAPCRRLR----------RGSNLKSDKMGNFLTQQYDRAK------ 104

Query: 1176 SREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDD-EATEEDVTGGSSIEEVDM 1000
             R A   +R+                    +K KEVI +DD E  EE V+  SS++E+  
Sbjct: 105  -RSAFETLRY-------------------VKKDKEVINIDDDEICEEGVSEDSSVKELGT 144

Query: 999  RIEKERPVFYGH--QWNTSTGIVE--NAVDGKRGVGNCKASMSSPVINMED--------- 859
             ++ +  +  G   QW  S G+VE  +  D  + +      +SS V+ + D         
Sbjct: 145  GVDAKMGLASGSGLQWKESNGVVEIMDNPDAVKDMDRNFQVLSSSVVTVSDGGMLKVENA 204

Query: 858  ---LSLKGLPLRLEPNKWCK--PPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSW--- 703
               L    L  + +        PP+  LLDSA KR+  L+ L F I+  EKR E      
Sbjct: 205  EKMLDTLSLSSKFDSASTSSSVPPYKKLLDSAEKRNDHLKRLQFHIEYTEKRRETQHLLR 264

Query: 702  SRXXXXXXXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCL 523
             +          +RE F+ L  E+ +E++RA + + SRRK+L  HE SNIDITGEILQCL
Sbjct: 265  PQKKEEYVKEDVIREPFMLLDEEEEAEVSRALSNS-SRRKVLAKHENSNIDITGEILQCL 323

Query: 522  RPGAWLNNEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTT 343
            RPGAWLN+EVINVYLELLKERE REP KFLKCHF NTFFYKKLI   GGYN+Q+V+RWT+
Sbjct: 324  RPGAWLNDEVINVYLELLKERERREPKKFLKCHFFNTFFYKKLISNKGGYNYQSVKRWTS 383

Query: 342  QRKLGYCLFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            QRKLGYCLF+CDKIFVPIHK+VHWCLAVINKKD+KFQYLDSLGG D +
Sbjct: 384  QRKLGYCLFECDKIFVPIHKQVHWCLAVINKKDEKFQYLDSLGGTDQQ 431



 Score = 92.4 bits (228), Expect(2) = e-103
 Identities = 40/59 (67%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y++DEV +K G  ID++SW+ EFVEDLPEQENG+DCG+FM+KYADFYSR  GL F Q
Sbjct: 437 ARYFIDEVKDKNGKYIDINSWKLEFVEDLPEQENGFDCGMFMLKYADFYSRDIGLCFSQ 495


>XP_012456061.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1
            [Gossypium raimondii] KJB72687.1 hypothetical protein
            B456_011G190600 [Gossypium raimondii]
          Length = 495

 Score =  315 bits (807), Expect(2) = e-103
 Identities = 207/457 (45%), Positives = 269/457 (58%), Gaps = 20/457 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDH-------------ISKKRRVSV---SSDNTST 1384
            MG L SNR RY     G  C + SNF H             +SKK R+S+   S +  + 
Sbjct: 1    MGALASNRKRY-----GDECFN-SNFCHKKKSPYSNSPEFQLSKKPRLSLMNESPEKANL 54

Query: 1383 RSRCSVYRFNLYPEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFD 1204
             S  +V R + YPEAK   PR VHAPV+  +F  S       G+ S    M N LS    
Sbjct: 55   SSNSTVLRISRYPEAKVSFPREVHAPVRRLKFGLSASK---PGQDSVESYMGNFLS---- 107

Query: 1203 ILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGG 1024
               S+ +   R+A G +RH  F+  E EV + V +  +KEKG   +V DD + EE     
Sbjct: 108  ---SRLSDARRQAWGALRH--FKK-EKEV-IFVEDDEEKEKG---LVSDDVSVEEV---- 153

Query: 1023 SSIEEVDMRIEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLKG 844
              IE+    +E+ER      Q ++S+ + E               +++  + ME  SL  
Sbjct: 154  ELIEKGKNNVEEERHF----QPSSSSAVTE---------------LNNGSLRMES-SLDM 193

Query: 843  LPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE----MSWSRXXXXXXX 676
            L LR   N +    +  L++SA +R   L++L FQI+L+EKR+     +   +       
Sbjct: 194  LSLREVSNGYDLEAYRKLIESAERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEEQH 253

Query: 675  XXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNE 496
                 E FIPL+ E+ + ++RA +  N R K+LVSHE S+IDI GEILQCL+PGAWLN+E
Sbjct: 254  EVLPTEPFIPLTEEEMALVSRALSVKNWR-KVLVSHENSSIDIRGEILQCLKPGAWLNDE 312

Query: 495  VINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLF 316
            VIN+YLELLKERENREP KFLKCHF NTFFYKKL+    GYN++AV+RWT+QRKLGYCL 
Sbjct: 313  VINLYLELLKERENREPKKFLKCHFCNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYCLL 372

Query: 315  DCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            DCDKIFVPIHK++HWCLAVINKKDQKFQYLDSL GRD
Sbjct: 373  DCDKIFVPIHKDIHWCLAVINKKDQKFQYLDSLKGRD 409



 Score = 90.9 bits (224), Expect(2) = e-103
 Identities = 39/59 (66%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+V+EV +K+G  ID+SSW+ EFVEDLP QENG+DCG+FM+KY DFYSRG  L F+Q
Sbjct: 417 AKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCGMFMLKYIDFYSRGLSLCFEQ 475


>XP_016677400.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Gossypium hirsutum]
          Length = 495

 Score =  313 bits (801), Expect(2) = e-102
 Identities = 205/459 (44%), Positives = 272/459 (59%), Gaps = 22/459 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDH-------------ISKKRRVSV---SSDNTST 1384
            MG L SNR RY     G  C + SNF H             +SKKRR+S+   S +  + 
Sbjct: 1    MGALASNRKRY-----GDECFN-SNFCHKKKSPYSNSPEFQLSKKRRLSLMNESPEKANL 54

Query: 1383 RSRCSVYRFNLYPEAKGELPRAVHAPVKNNRF--SFSKRGLFGSGKVSSADKMLNSLSNT 1210
             S  +V R + YPE+K   PR VHAPV+  +F  S SK G          D + + + N 
Sbjct: 55   SSNSTVLRISRYPESKFSFPREVHAPVRRLKFWLSASKPG---------QDSVESYMGN- 104

Query: 1209 FDILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVT 1030
               L S+ +   R+A G +RH  F+  E EV + V +  +KEKG   +V DD + EE   
Sbjct: 105  --FLSSRLSDAKRQAWGALRH--FKK-EKEV-IFVEDDEEKEKG---LVSDDVSVEEV-- 153

Query: 1029 GGSSIEEVDMRIEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSL 850
                IE+    +++ER      Q ++S+ + E               +++  + ME  SL
Sbjct: 154  --ELIEKGKNNVKEERHF----QPSSSSAVTE---------------LNNGSLRMES-SL 191

Query: 849  KGLPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE----MSWSRXXXXX 682
              L LR   N +    +  L++SA +R   L++L FQI+L+EKR+     +   +     
Sbjct: 192  DMLSLREVSNGYDLEAYRKLIESAERRSSKLKDLGFQIELNEKRIAGLQALRPEKKPEEE 251

Query: 681  XXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLN 502
                   E FIPL+ E+ + ++RA + A + RK+LVSHE S+IDI GEILQCL+PGAWLN
Sbjct: 252  QHEVLPTEPFIPLTEEEMALVSRALS-AKNWRKVLVSHENSSIDIRGEILQCLKPGAWLN 310

Query: 501  NEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYC 322
            +EVIN+YLELLKERENREP KFLKCHF NTFFYKKL+    GYN++AV+RWT+QRKLGYC
Sbjct: 311  DEVINLYLELLKERENREPKKFLKCHFFNTFFYKKLVNPESGYNYRAVKRWTSQRKLGYC 370

Query: 321  LFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            L DCDKIFVPIHK++HWCLAVINKK QKFQYLDSL GRD
Sbjct: 371  LLDCDKIFVPIHKDIHWCLAVINKKYQKFQYLDSLKGRD 409



 Score = 90.9 bits (224), Expect(2) = e-102
 Identities = 39/59 (66%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+V+EV +K+G  ID+SSW+ EFVEDLP QENG+DCG+FM+KY DFYSRG  L F+Q
Sbjct: 417 AKYFVEEVKDKSGKDIDISSWEQEFVEDLPAQENGFDCGMFMLKYIDFYSRGLSLCFEQ 475


>XP_018825732.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Juglans regia]
          Length = 524

 Score =  306 bits (783), Expect(2) = e-102
 Identities = 203/469 (43%), Positives = 264/469 (56%), Gaps = 30/469 (6%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFD--------HISKKRRVSV---SSDNTSTRSRCS 1369
            MG   +NR R      G  CL  ++          H+SKK R S    + D     S  +
Sbjct: 1    MGARTTNRKR------GDECLSFNHTYPYQNSLDFHVSKKPRFSSIHQTPDRPIVSSNST 54

Query: 1368 VYRFNLYPEAKGELPRAVHAPVKNNRFSFSKRGLF-GSGKVSSA----DKMLNSLSNTFD 1204
            V R + YPEA   L R VHAP +   + FS   L   SG  +S     ++ L+ + +T  
Sbjct: 55   VARLSRYPEATPRLGREVHAPCRILNYGFSSTCLNRNSGPRTSGIYIKEEPLDEMGST-- 112

Query: 1203 ILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLD-DEATEEDVTG 1027
             L  KY K    AL  IR+                   KEK KEVI +D D      V+ 
Sbjct: 113  -LSYKYNKAKNIALRTIRY-----------------MTKEKEKEVIEIDTDNQKYGGVSE 154

Query: 1026 GSSIEEVDMRIEKE--RPVFYGHQWNTSTGIVENAVDGK-----RGVGNCKASMSSPVIN 868
             SSIEEV++  +    R     H+     G+V N  D       RG+    +S  S + N
Sbjct: 155  DSSIEEVEVLEDGRGGRSEVSEHR-KVDNGVVANVQDAHAKVLDRGLLPSSSSAVSDLTN 213

Query: 867  MEDLSLKGLP-----LRLEPNK-WCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMS 706
              +L +         L+L P +         LL+ A +R+P L+ L+F+I+L+E+R  M 
Sbjct: 214  GNNLRVDDAEKMLDSLQLNPERDMSVSAFRKLLEVAERRNPKLRSLSFEIELNEERRSMF 273

Query: 705  WSRXXXXXXXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQC 526
             S             E F+PL+ E+ +E+ RAF   ++RRK+LV+HE SNI+ITGEILQC
Sbjct: 274  QSLRPAKKPVEDVPGEPFLPLTKEEEAEVERAFN--SNRRKVLVTHENSNIEITGEILQC 331

Query: 525  LRPGAWLNNEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWT 346
            LRPGAWLN+EVINVYLELLKERENREP K+LKCHF NTFFYKKLI G  GY++++VRRWT
Sbjct: 332  LRPGAWLNDEVINVYLELLKERENREPQKYLKCHFFNTFFYKKLISGKNGYDYKSVRRWT 391

Query: 345  TQRKLGYCLFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            +QRKLGY L  CDKIFVPIH+E+HWCLAVIN+KD+KFQYLDSL G D+K
Sbjct: 392  SQRKLGYGLIHCDKIFVPIHREIHWCLAVINRKDEKFQYLDSLRGMDNK 440



 Score = 96.3 bits (238), Expect(2) = e-102
 Identities = 43/59 (72%), Positives = 50/59 (84%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  +DVSSW+ EFVEDLPEQENG+DCG+FMIKYADFYSR  GL F Q
Sbjct: 446 ARYFVDEVKDKSGKDVDVSSWKQEFVEDLPEQENGFDCGMFMIKYADFYSRDIGLCFNQ 504


>XP_009612674.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1
            [Nicotiana tomentosiformis]
          Length = 517

 Score =  308 bits (790), Expect(2) = e-102
 Identities = 200/454 (44%), Positives = 255/454 (56%), Gaps = 17/454 (3%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSN---FDHISKKRRVSVSSDNTSTRSRCSVYRFNLYP 1345
            MG L  NR R    F  +     SN   FDHISKK ++S+    T    + +V RF  YP
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQ--TLNPPKSTVQRFLKYP 58

Query: 1344 EAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVS--SADKMLNSLSNTFDILKSKYAKISR 1171
            +    + R VHAP + +RF        GS  +S  SA +M N +S  +D  K K A  S 
Sbjct: 59   DPINPIRRQVHAPCRKSRF--------GSKCISANSAPEMGNFVSRVYDEAK-KSAFYSL 109

Query: 1170 EALGVIRHGVFRDVED----EVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVD 1003
              L  ++  +F D +D    ++  GV          E  V +      ++ GG       
Sbjct: 110  RCLKKVKEVIFIDDDDAKKGDLEAGVYEKSGFYGNLEAGVCEKNNFFGNLEGGVG----- 164

Query: 1002 MRIEKERPVFYGHQWNTSTGIVE--NAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRL 829
                 E+ V    +   + G VE  + VDGK       A +    +  E +      L L
Sbjct: 165  -----EKSVILSQEGKETNGAVEILDGVDGKDS-SVVTAGLDDGNLKEESVGKMLDTLAL 218

Query: 828  EPNK---WCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE---MSWSRXXXXXXXXXX 667
             P     +  P +  LL S  K +  L+ L FQI  +EK LE   +   +          
Sbjct: 219  NPTSDSAFYVPLYKKLLGSVGKMNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDV 278

Query: 666  VREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVIN 487
            + E F+PL+ ED +E+ RA +K+N RR++LV+H+ SNIDITGEILQCLRP  WLN+EVIN
Sbjct: 279  ISEPFVPLTEEDEAEVLRALSKSN-RRRVLVTHQNSNIDITGEILQCLRPREWLNDEVIN 337

Query: 486  VYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCD 307
            VYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGYCL +CD
Sbjct: 338  VYLELLKEREKREPQKFLKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECD 397

Query: 306  KIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            KIFVPIHKE+HWCLAVINKKD+KFQYLDSL GRD
Sbjct: 398  KIFVPIHKEIHWCLAVINKKDEKFQYLDSLRGRD 431



 Score = 92.8 bits (229), Expect(2) = e-102
 Identities = 43/59 (72%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NG+DCGVFMIK ADFYSR  GL F Q
Sbjct: 439 ARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVFMIKNADFYSRDIGLCFNQ 497


>XP_018629573.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X3
            [Nicotiana tomentosiformis]
          Length = 503

 Score =  308 bits (790), Expect(2) = e-102
 Identities = 201/450 (44%), Positives = 256/450 (56%), Gaps = 13/450 (2%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSN---FDHISKKRRVSVSSDNTSTRSRCSVYRFNLYP 1345
            MG L  NR R    F  +     SN   FDHISKK ++S+    T    + +V RF  YP
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQ--TLNPPKSTVQRFLKYP 58

Query: 1344 EAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVS--SADKMLNSLSNTFDILKSKYAKISR 1171
            +    + R VHAP + +RF        GS  +S  SA +M N +S  +D  K K A  S 
Sbjct: 59   DPINPIRRQVHAPCRKSRF--------GSKCISANSAPEMGNFVSRVYDEAK-KSAFYSL 109

Query: 1170 EALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMRIE 991
              L  ++  +F D +D+ + G     D E G    V +      ++ GG           
Sbjct: 110  RCLKKVKEVIFID-DDDAKKG-----DLEAG----VCEKNNFFGNLEGGVG--------- 150

Query: 990  KERPVFYGHQWNTSTGIVE--NAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRLEPNK 817
             E+ V    +   + G VE  + VDGK       A +    +  E +      L L P  
Sbjct: 151  -EKSVILSQEGKETNGAVEILDGVDGKDS-SVVTAGLDDGNLKEESVGKMLDTLALNPTS 208

Query: 816  ---WCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE---MSWSRXXXXXXXXXXVREA 655
               +  P +  LL S  K +  L+ L FQI  +EK LE   +   +          + E 
Sbjct: 209  DSAFYVPLYKKLLGSVGKMNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDVISEP 268

Query: 654  FIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVINVYLE 475
            F+PL+ ED +E+ RA +K+N RR++LV+H+ SNIDITGEILQCLRP  WLN+EVINVYLE
Sbjct: 269  FVPLTEEDEAEVLRALSKSN-RRRVLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLE 327

Query: 474  LLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCDKIFV 295
            LLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGYCL +CDKIFV
Sbjct: 328  LLKEREKREPQKFLKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECDKIFV 387

Query: 294  PIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            PIHKE+HWCLAVINKKD+KFQYLDSL GRD
Sbjct: 388  PIHKEIHWCLAVINKKDEKFQYLDSLRGRD 417



 Score = 92.8 bits (229), Expect(2) = e-102
 Identities = 43/59 (72%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NG+DCGVFMIK ADFYSR  GL F Q
Sbjct: 425 ARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVFMIKNADFYSRDIGLCFNQ 483


>XP_016542482.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1 [Capsicum
            annuum]
          Length = 509

 Score =  310 bits (794), Expect(2) = e-101
 Identities = 196/454 (43%), Positives = 253/454 (55%), Gaps = 17/454 (3%)
 Frame = -3

Query: 1515 MGVLI-SNRNRYEAHFRGSLCLDLS---NFDHISKKRRVSVSSDNTSTRSRCSVYRFNLY 1348
            MG L  +NR R +  F  +    LS   NFDHISKK R+     +     + +V R   Y
Sbjct: 1    MGALTCTNRKRGDDFFTSNYKTPLSVSSNFDHISKKPRLMNQIHDHPIPRKSTVTRIFKY 60

Query: 1347 PEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKISRE 1168
            P     + R +HAP + ++  F           S+ DKM N L+  +D  K       R 
Sbjct: 61   PSTITPIKREIHAPCRRSKIGF----------YSNFDKMGNFLAQQYDRAK-------RS 103

Query: 1167 ALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMRIEK 988
            A   +R+                    +K KEVI +DD+  E++      +E V    ++
Sbjct: 104  AYETLRY-------------------VKKDKEVIKIDDDDDEKNCGKELELENVFFA-KR 143

Query: 987  ERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRLEP----- 823
             R    G +   S G+VE   +  R       S  + V+N  +L  +     L+      
Sbjct: 144  GRASGSGQKREESNGVVEIMDNSDRNFQVSSFSAVTTVLNDGNLKAENTEKMLDSLSLSG 203

Query: 822  -----NKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSW---SRXXXXXXXXXX 667
                 +    P +  LLDSA KR+  L+ L FQI+  EK  E      S+          
Sbjct: 204  KFDDDSSSSVPSYKKLLDSAEKRNDNLKRLQFQIEYTEKLRETQHLLRSQQKEEYVKEDV 263

Query: 666  VREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVIN 487
            + E F+ L+ E+ +E++RA + +N RRK+LV+HE SNIDITGE LQCLRPGAWLN+EVIN
Sbjct: 264  IIEPFVRLAEEEEAEVSRALSNSN-RRKVLVTHENSNIDITGEKLQCLRPGAWLNDEVIN 322

Query: 486  VYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCD 307
            VYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q V+RWT+QRKLGY LF+CD
Sbjct: 323  VYLELLKEREKREPEKFLKCHFFNTFFYKKLISGKGGYNYQPVKRWTSQRKLGYSLFECD 382

Query: 306  KIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            KIFVPIHK+VHWCLAVINKKD+KFQYLDSLGGRD
Sbjct: 383  KIFVPIHKQVHWCLAVINKKDEKFQYLDSLGGRD 416



 Score = 90.5 bits (223), Expect(2) = e-101
 Identities = 40/59 (67%), Positives = 47/59 (79%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y++DEV +K G  IDV SW+ EFVEDLPEQENG+DCG+FM+KY DFYSR  GL F Q
Sbjct: 424 ARYFIDEVKDKNGKDIDVRSWKLEFVEDLPEQENGFDCGMFMLKYTDFYSRDIGLCFNQ 482


>XP_016542489.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X2 [Capsicum
            annuum]
          Length = 502

 Score =  310 bits (794), Expect(2) = e-101
 Identities = 196/454 (43%), Positives = 253/454 (55%), Gaps = 17/454 (3%)
 Frame = -3

Query: 1515 MGVLI-SNRNRYEAHFRGSLCLDLS---NFDHISKKRRVSVSSDNTSTRSRCSVYRFNLY 1348
            MG L  +NR R +  F  +    LS   NFDHISKK R+     +     + +V R   Y
Sbjct: 1    MGALTCTNRKRGDDFFTSNYKTPLSVSSNFDHISKKPRLMNQIHDHPIPRKSTVTRIFKY 60

Query: 1347 PEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKISRE 1168
            P     + R +HAP + ++  F           S+ DKM N L+  +D  K       R 
Sbjct: 61   PSTITPIKREIHAPCRRSKIGF----------YSNFDKMGNFLAQQYDRAK-------RS 103

Query: 1167 ALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMRIEK 988
            A   +R+                    +K KEVI +DD+  E++      +E V    ++
Sbjct: 104  AYETLRY-------------------VKKDKEVIKIDDDDDEKNCGKELELENVFFA-KR 143

Query: 987  ERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRLEP----- 823
             R    G +   S G+VE   +  R       S  + V+N  +L  +     L+      
Sbjct: 144  GRASGSGQKREESNGVVEIMDNSDRNFQVSSFSAVTTVLNDGNLKAENTEKMLDSLSLSG 203

Query: 822  -----NKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSW---SRXXXXXXXXXX 667
                 +    P +  LLDSA KR+  L+ L FQI+  EK  E      S+          
Sbjct: 204  KFDDDSSSSVPSYKKLLDSAEKRNDNLKRLQFQIEYTEKLRETQHLLRSQQKEEYVKEDV 263

Query: 666  VREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVIN 487
            + E F+ L+ E+ +E++RA + +N RRK+LV+HE SNIDITGE LQCLRPGAWLN+EVIN
Sbjct: 264  IIEPFVRLAEEEEAEVSRALSNSN-RRKVLVTHENSNIDITGEKLQCLRPGAWLNDEVIN 322

Query: 486  VYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCD 307
            VYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q V+RWT+QRKLGY LF+CD
Sbjct: 323  VYLELLKEREKREPEKFLKCHFFNTFFYKKLISGKGGYNYQPVKRWTSQRKLGYSLFECD 382

Query: 306  KIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            KIFVPIHK+VHWCLAVINKKD+KFQYLDSLGGRD
Sbjct: 383  KIFVPIHKQVHWCLAVINKKDEKFQYLDSLGGRD 416



 Score = 90.5 bits (223), Expect(2) = e-101
 Identities = 40/59 (67%), Positives = 47/59 (79%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y++DEV +K G  IDV SW+ EFVEDLPEQENG+DCG+FM+KY DFYSR  GL F Q
Sbjct: 424 ARYFIDEVKDKNGKDIDVRSWKLEFVEDLPEQENGFDCGMFMLKYTDFYSRDIGLCFNQ 482


>XP_014510318.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1 [Vigna
            radiata var. radiata]
          Length = 518

 Score =  301 bits (772), Expect(2) = e-101
 Identities = 193/432 (44%), Positives = 246/432 (56%), Gaps = 24/432 (5%)
 Frame = -3

Query: 1428 SKKRRVSVSSDNTS---TRSRCSVYRFNLYPEAKGELPRAVHAPVKNNRFSFS-----KR 1273
            SK+ R+S +SDN +     S   V R + YPEAK  + R VHAP +  +F F      K 
Sbjct: 26   SKRLRLSATSDNHAHSVLSSSSIVSRISKYPEAKPLVRREVHAPCRPRKFDFPTFNRMKS 85

Query: 1272 GLFGSGKV-SSADKMLNSLSNTFDILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNW 1096
             L  SG      D+++N + NT   L   Y +    AL  IR G                
Sbjct: 86   SLNASGSYYDRKDEIVNDVGNT---LNENYERAKNSALASIRFG---------------- 126

Query: 1095 RDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMRIEKE--RPVFYGHQWNTSTGIVENAVD 922
               EKGKEVI +DD + +  V+  S +EEV  R +    R V   H+W     +V    D
Sbjct: 127  ---EKGKEVIEVDDGSPKGMVSEDSGVEEVHFREDGRDVRSVVTEHKWQEGDLVVTEVKD 183

Query: 921  GK----RGVGNCKASMSSPVINMEDLSLKGL-------PLRLEPNKWCKPPHG--DLLDS 781
             K    RG G  + S SS V  + +  L  +        L L P +     H    LL++
Sbjct: 184  MKAKDVRG-GPQQQSTSSVVSELTNGDLNVVNAVKMFGTLSLTPERDVSDVHAYRKLLEA 242

Query: 780  ARKRDPILQELAFQIQLHEKRLEMSWSRXXXXXXXXXXVREAFIPLSAEDRSELARAFAK 601
              KR   L+ L+ +I+L+EKR                   E FIPL+ E+  E+ RAF+ 
Sbjct: 243  VDKRSDTLKRLSAEIKLNEKRRSTFELLRPKKELVQEVPFEPFIPLTEEEEVEVTRAFSA 302

Query: 600  ANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVINVYLELLKERENREPHKFLKCHF 421
              +RRKILV+HEKS I+++GE  QCLRPGAWLN+EVIN+YLELLKERE REP KFLKCHF
Sbjct: 303  --NRRKILVAHEKSCIEVSGEKFQCLRPGAWLNDEVINLYLELLKERERREPQKFLKCHF 360

Query: 420  LNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCDKIFVPIHKEVHWCLAVINKKDQ 241
             NTFFYKKLI G  GY+F++VRRWT+Q+KLGY L +CDKIFVPIHKE+HWCLAVINKK++
Sbjct: 361  FNTFFYKKLISGKNGYDFKSVRRWTSQKKLGYGLHECDKIFVPIHKEIHWCLAVINKKEK 420

Query: 240  KFQYLDSLGGRD 205
            KFQYLDSL G D
Sbjct: 421  KFQYLDSLKGVD 432



 Score = 98.6 bits (244), Expect(2) = e-101
 Identities = 44/59 (74%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y VDEV +KTG  +D+SSW+ EFVEDLPEQ+NGYDCGVFMIKYADFYSR  GL F Q
Sbjct: 440 ASYIVDEVKDKTGKDMDISSWEKEFVEDLPEQQNGYDCGVFMIKYADFYSRNSGLCFNQ 498


>XP_004229242.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum] XP_010321351.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum] XP_010321353.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Solanum
            lycopersicum]
          Length = 515

 Score =  307 bits (787), Expect(2) = e-101
 Identities = 203/472 (43%), Positives = 260/472 (55%), Gaps = 33/472 (6%)
 Frame = -3

Query: 1515 MGVLI-SNRNRYEAHFRGSLCLDLS---NFDHISKKRRVSVSSDNTSTR--SRCSVY-RF 1357
            MG L  S+R R +  F  +    LS   N DHISKK R+S S +       SR S+  R 
Sbjct: 1    MGALTCSSRKRGDDFFNSNYKTPLSVSSNVDHISKKPRLSPSMNQIQDHPFSRKSIAARI 60

Query: 1356 NLYPEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKI 1177
              YP     + R +HAP +  R           G    +DKM N L+  +D  K      
Sbjct: 61   FKYPSNITPIKREIHAPCRRLR----------RGSNLKSDKMGNFLTQQYDRAK------ 104

Query: 1176 SREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDD-EATEEDVTGGSSIEEVDM 1000
             R A   +R+                    +K KEVI +DD E  EE V+  SS++E+  
Sbjct: 105  -RSAFETLRY-------------------VKKDKEVINIDDDEICEEGVSEDSSVKELGT 144

Query: 999  RIEKERPVFYGH--QWNTSTGIVE--NAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLR 832
             ++ +  +  G   QW  S G+VE  +  D  + +      +SS V+ + D    G+ L+
Sbjct: 145  GVDAKTGLASGSGSQWKESNGVVEIMDNPDAVKDMDRNFQVLSSSVVTVSD----GVKLK 200

Query: 831  LE------------------PNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMS 706
            +E                        PP+  LL SA KR+  L+ L F I+  EKR E  
Sbjct: 201  VENAEKMLDTLSLSSKFDSASTSSSVPPYKKLLGSAEKRNDHLKRLQFHIEYTEKRRETQ 260

Query: 705  W---SRXXXXXXXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEI 535
                 +          + E F+ L  E+ +E++RA + + SRRK+L  HE SNIDITGEI
Sbjct: 261  HLLRPQKKEEYVKEDVITEPFMLLDEEEEAEVSRALSNS-SRRKVLAKHENSNIDITGEI 319

Query: 534  LQCLRPGAWLNNEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVR 355
            LQCLRPGAWLN+EVINVYLELLKERE REP KFLKCHF NTFFYKKLI   G YN+Q+V+
Sbjct: 320  LQCLRPGAWLNDEVINVYLELLKERERREPKKFLKCHFFNTFFYKKLISNKGDYNYQSVK 379

Query: 354  RWTTQRKLGYCLFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            RWT+QRKLGYCLF+CDKIFVPIHK+VHWCLAVINKKD+KFQYLDSLGG D +
Sbjct: 380  RWTSQRKLGYCLFECDKIFVPIHKQVHWCLAVINKKDEKFQYLDSLGGTDQQ 431



 Score = 92.4 bits (228), Expect(2) = e-101
 Identities = 40/59 (67%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y++DEV +K G  ID++SW+ EFVEDLPEQENG+DCG+FM+KYADFYSR  GL F Q
Sbjct: 437 ARYFIDEVKDKNGKYIDINSWKLEFVEDLPEQENGFDCGMFMLKYADFYSRDIGLCFSQ 495


>CDO98863.1 unnamed protein product [Coffea canephora]
          Length = 511

 Score =  304 bits (779), Expect(2) = e-101
 Identities = 199/461 (43%), Positives = 257/461 (55%), Gaps = 22/461 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDHISKKRRVSVSSDNTSTRSR--CSVYRFNLYPE 1342
            MG L SNR R            L +   +SKK R+  S + T+ R+    +V++F  YP 
Sbjct: 1    MGALTSNRKRGYDFSSSVNYGSLVSNSPVSKKSRLPASINQTTGRTDRITAVFQFFRYPV 60

Query: 1341 AKGELPRAVHAPVKNNRFSFSKRGLFGSG-----KVSSADKMLNSLSNTFDILKSKYAKI 1177
             K    R  HAPV+ +R   S   L  S      KV SAD+M N LS        +Y   
Sbjct: 61   EKTLFKREPHAPVRRHR-GVSSVNLGNSASKVSYKVGSADEMGNLLSG-------QYKST 112

Query: 1176 SREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMR 997
             R AL  +R                     E+ K+V  +  E  +E V+  SSIEEV++ 
Sbjct: 113  KRSALDTLRFN-------------------EEDKKVTEVAKEEVQE-VSEDSSIEEVEIL 152

Query: 996  IEKERPVFYGHQWNTSTGIVEN---AVDGKRGVGNCKASMSSPVINMEDLSLK------- 847
              +E       +W    G+V+      DG     + + S SS + N+ D  LK       
Sbjct: 153  EVRE-----DQKWKDGNGVVDEDSRKFDGIAVDKDSRPSSSSAMTNVSDGILKVETTEKL 207

Query: 846  --GLPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSW---SRXXXXX 682
               L L  E     +  H  LL  A +R+  +  L FQI+  EK+L++      +     
Sbjct: 208  LESLSLSQELGVPQESVHKKLLYVAERRNDKINSLNFQIEYTEKQLQLQQLLRPQKKEEA 267

Query: 681  XXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLN 502
                   EAF PL+ E+ +E+ RA + + SRRK+LV+HE SNI ITGE+LQCLRP AWLN
Sbjct: 268  AKKDVTAEAFKPLTEEEETEVNRALSNS-SRRKLLVTHENSNISITGEVLQCLRPRAWLN 326

Query: 501  NEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYC 322
            +EVIN+YLELLKERE REP KFL CHF NTFF+KKL+ G GGYN+Q+VRRWT+Q+KLGYC
Sbjct: 327  DEVINLYLELLKERERREPKKFLNCHFFNTFFFKKLVSGKGGYNYQSVRRWTSQKKLGYC 386

Query: 321  LFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            +FDCDKIFVPIHKEVHWCLAVINKKD+KFQYLDSLGG D +
Sbjct: 387  IFDCDKIFVPIHKEVHWCLAVINKKDEKFQYLDSLGGVDSQ 427



 Score = 95.5 bits (236), Expect(2) = e-101
 Identities = 45/59 (76%), Positives = 47/59 (79%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y VDEV +K G  IDVSSWQ EFV DLPEQENG+DCGVFMIKYADFYSR  GL F Q
Sbjct: 433 ARYIVDEVKDKCGKGIDVSSWQQEFVADLPEQENGFDCGVFMIKYADFYSRDIGLCFNQ 491


>XP_009612677.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X2
            [Nicotiana tomentosiformis]
          Length = 516

 Score =  306 bits (784), Expect(2) = e-101
 Identities = 199/454 (43%), Positives = 254/454 (55%), Gaps = 17/454 (3%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSN---FDHISKKRRVSVSSDNTSTRSRCSVYRFNLYP 1345
            MG L  NR R    F  +     SN   FDHISKK ++S+    T    + +V RF  YP
Sbjct: 1    MGALTFNRKRGNDFFSSNYKTQFSNPSNFDHISKKPKLSIYQ--TLNPPKSTVQRFLKYP 58

Query: 1344 EAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVS--SADKMLNSLSNTFDILKSKYAKISR 1171
            +    + R VHAP + +RF        GS  +S  SA +M N +S  +D  K K A  S 
Sbjct: 59   DPINPIRRQVHAPCRKSRF--------GSKCISANSAPEMGNFVSRVYDEAK-KSAFYSL 109

Query: 1170 EALGVIRHGVFRDVED----EVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVD 1003
              L  ++  +F D +D    ++  GV          E  V +      ++ GG       
Sbjct: 110  RCLKKVKEVIFIDDDDAKKGDLEAGVYEKSGFYGNLEAGVCEKNNFFGNLEGGVG----- 164

Query: 1002 MRIEKERPVFYGHQWNTSTGIVE--NAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRL 829
                 E+ V    +   + G VE  + VDGK       A +    +  E +      L L
Sbjct: 165  -----EKSVILSQEGKETNGAVEILDGVDGKDS-SVVTAGLDDGNLKEESVGKMLDTLAL 218

Query: 828  EPNK---WCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE---MSWSRXXXXXXXXXX 667
             P     +  P +  LL S  K +  L+ L FQI  +EK LE   +   +          
Sbjct: 219  NPTSDSAFYVPLYKKLLGSVGKMNDKLKRLQFQIGYNEKCLETYSLLRPQKKGEHVKQDV 278

Query: 666  VREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVIN 487
            + E F+PL+ ED +E+ RA +K+N  R++LV+H+ SNIDITGEILQCLRP  WLN+EVIN
Sbjct: 279  ISEPFVPLTEEDEAEVLRALSKSN--RRVLVTHQNSNIDITGEILQCLRPREWLNDEVIN 336

Query: 486  VYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCD 307
            VYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGYCL +CD
Sbjct: 337  VYLELLKEREKREPQKFLKCHFFNTFFYKKLISGRGGYNYQSVRRWTSQRKLGYCLLECD 396

Query: 306  KIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            KIFVPIHKE+HWCLAVINKKD+KFQYLDSL GRD
Sbjct: 397  KIFVPIHKEIHWCLAVINKKDEKFQYLDSLRGRD 430



 Score = 92.8 bits (229), Expect(2) = e-101
 Identities = 43/59 (72%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NG+DCGVFMIK ADFYSR  GL F Q
Sbjct: 438 ARYFVDEVKDKSGKDIDVSSWKQEFVEDLPEQKNGFDCGVFMIKNADFYSRDIGLCFNQ 496


>XP_010039753.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Eucalyptus grandis]
            XP_010039756.1 PREDICTED: ubiquitin-like-specific
            protease ESD4 [Eucalyptus grandis] KCW84789.1
            hypothetical protein EUGRSUZ_B01592 [Eucalyptus grandis]
            KCW84790.1 hypothetical protein EUGRSUZ_B01592
            [Eucalyptus grandis]
          Length = 502

 Score =  308 bits (788), Expect(2) = e-100
 Identities = 197/452 (43%), Positives = 254/452 (56%), Gaps = 13/452 (2%)
 Frame = -3

Query: 1515 MGVLISNRNR---YEAHFRGSLCLDLSNFDHISKKRRV---SVSSDNTSTRSRCSVYRFN 1354
            MG L  NR R   + +       L  S   H SK+ R+      S   +  S+ +V R  
Sbjct: 1    MGALTGNRKRGDEFSSLSNRETKLHGSPGSHASKRPRLFSPMQQSPGRAVPSKSAVSRIA 60

Query: 1353 LYPEAKGELPRAVHAPVKNNRFSFSKRGL----FGSGKVSSADKMLNSLSNTFDILKSKY 1186
             YPEA  +L R VHAPV+ ++F  S   L    FG G  SS D M N        +  KY
Sbjct: 61   RYPEAASKLRREVHAPVRTSKFGSSASALGRISFGKGDYSS-DYMGN--------IMYKY 111

Query: 1185 AKISREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVI-VLDDEATEEDVTGGSSIEE 1009
             K    ALG +R+                     + KEVI ++DDE  +E V+  SSIEE
Sbjct: 112  QKAKAGALGSLRYFT-------------------REKEVIDLVDDEPRKEIVSLDSSIEE 152

Query: 1008 VD-MRIEKERPVFYGHQWNTSTGIVENAVDG-KRGVGNCKASMSSPVINMEDLSLKGLPL 835
            +D     +E  V  G +      +  NA +     + N  A + +P   M  LSL     
Sbjct: 153  IDGFEDGRETSVASGSRSKDENQVATNAQESIVSELSNGNAGVETPEKMMYRLSLNAD-- 210

Query: 834  RLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSWSRXXXXXXXXXXVREA 655
              +P    K  +  LL++A++RD  L  L F+I+LHE++  +                E 
Sbjct: 211  --DPEILSKSAYQQLLENAKRRDAKLGSLDFEIRLHEEKRSLFQELRPAKKPDEKVPSEP 268

Query: 654  FIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVINVYLE 475
            FIPL+ E+ +E+ RA +   +RRK LV HE S I+I GE L CLRPGAWLN+EVIN+YLE
Sbjct: 269  FIPLTDEEEAEVQRALS--GNRRKALVYHEDSGIEIIGETLLCLRPGAWLNDEVINLYLE 326

Query: 474  LLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCDKIFV 295
            LLKERE R+P KFLKCHF NTFFYKKLI G  GY+++AVRRWTTQ+KLGY L DCDKIFV
Sbjct: 327  LLKEREKRDPRKFLKCHFFNTFFYKKLISGRSGYDYKAVRRWTTQKKLGYNLIDCDKIFV 386

Query: 294  PIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            PIH+E+HWCLA+INKKD+KFQYLDSLGGRD +
Sbjct: 387  PIHQEIHWCLAIINKKDEKFQYLDSLGGRDSR 418



 Score = 89.7 bits (221), Expect(2) = e-100
 Identities = 41/59 (69%), Positives = 46/59 (77%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y VDEV +K G  IDVSSW+ E+V DLP QENG+DCG+FMIKYADFYSRG  L F Q
Sbjct: 424 ARYVVDEVKDKCGKVIDVSSWEQEYVSDLPAQENGFDCGMFMIKYADFYSRGFELCFDQ 482


>XP_019196477.1 PREDICTED: putative ubiquitin-like-specific protease 1B isoform X1
            [Ipomoea nil] XP_019196478.1 PREDICTED: putative
            ubiquitin-like-specific protease 1B isoform X1 [Ipomoea
            nil] XP_019196479.1 PREDICTED: putative
            ubiquitin-like-specific protease 1B isoform X2 [Ipomoea
            nil]
          Length = 482

 Score =  301 bits (772), Expect(2) = e-100
 Identities = 188/446 (42%), Positives = 245/446 (54%), Gaps = 9/446 (2%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDHISKKRRVSVSSDNTSTRS-------RCSVYRF 1357
            M  L  NR R E  F  +      NFDH+SKK ++S S  +    S       + ++ RF
Sbjct: 1    MEALAPNRIRVEDFFTSNFDTLSPNFDHVSKKPKLSPSPAHQPPDSAAPHISAKSTISRF 60

Query: 1356 NLYPEAKGELPRAVHAPVKNNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKI 1177
              YPE   +L R VHAP +  RF FS      SGKV   ++  N  S +  +L  +Y + 
Sbjct: 61   FRYPEVTAQLKRQVHAPCRQTRFRFSANSRNFSGKVDFGEE--NPGSRSGSLLSKRYERA 118

Query: 1176 SREALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDMR 997
               A   +R+   R  ED + V        EK +      D   E ++ G S   E +  
Sbjct: 119  KESAFRCLRY--VRKEEDVIEVDNEEVEVVEKVRVEYPPKDGDEEVEILGASVRNEDE-- 174

Query: 996  IEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLKGLPLRLEPNK 817
             EKER +                          K S SS V  +++L L           
Sbjct: 175  -EKERTL--------------------------KPSSSSGVTELDNLDL----------- 196

Query: 816  WCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLE--MSWSRXXXXXXXXXXVREAFIPL 643
               P +  LLD+  K+D + + L F I+L EKR     +  +          ++E F+ L
Sbjct: 197  ---PSYKKLLDAVNKKDDMFKRLKFDIELLEKRRLHLQTQKKAGEVKEVLCLIQEPFVSL 253

Query: 642  SAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVINVYLELLKE 463
            + E+ +E+A A + +N R  +LV+HE SNIDITG++LQCL+PGAWLN+EVINVYLELLKE
Sbjct: 254  TEEEDAEVACALSNSN-RHNVLVTHENSNIDITGKLLQCLKPGAWLNDEVINVYLELLKE 312

Query: 462  RENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCDKIFVPIHK 283
            RE +EP KFL+CHF NTFFYK+L    GGYN+Q+VRRWT+QRKLGYCL +CDKIFVPIHK
Sbjct: 313  REKQEPQKFLRCHFFNTFFYKRLT--RGGYNYQSVRRWTSQRKLGYCLLECDKIFVPIHK 370

Query: 282  EVHWCLAVINKKDQKFQYLDSLGGRD 205
            EVHWCLAVINKKD KFQYLDSL GRD
Sbjct: 371  EVHWCLAVINKKDGKFQYLDSLRGRD 396



 Score = 95.5 bits (236), Expect(2) = e-100
 Identities = 42/59 (71%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NG+DCG+FMIKY DFYSR  GL F Q
Sbjct: 404 AKYFVDEVKDKSGKLIDVSSWEQEFVEDLPEQQNGFDCGMFMIKYVDFYSRDKGLCFSQ 462


>XP_009598750.1 PREDICTED: ubiquitin-like-specific protease ESD4 isoform X1
            [Nicotiana tomentosiformis]
          Length = 528

 Score =  305 bits (781), Expect(2) = e-100
 Identities = 195/477 (40%), Positives = 256/477 (53%), Gaps = 38/477 (7%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFD-HISKKRRVSVSSDNTSTRSRCSVYR-FNLYPE 1342
            MG L SNR R +  F  +     SN D HISKK R+S++          +V R F  YP 
Sbjct: 1    MGALTSNRKRGDDFFTLNYKTPASNVDPHISKKPRLSMNQTTQDHSKTSTVKRLFIKYPS 60

Query: 1341 AKGELPRAVHAPVK---NNRFSFSKRGLFGSGKVSSADKMLNSLSNTFDILKSKYAKISR 1171
            +   + R +HAP K   ++RF      + G+  V    +  +S   TF  LK K      
Sbjct: 61   SITSIKREIHAPCKRPSSSRFGGGSHSIMGNFLVQQLYRAKSSAFKTFRYLKKK------ 114

Query: 1170 EALGVIRHGVFRDVEDEVRVGVPNWRDKEKGKEVIVLDDEATEEDVTGGSSIEEVDM--- 1000
                          + +V + V               DDE +  +  G   + +  +   
Sbjct: 115  --------------DKQVVINVDG-------------DDEDSHVEELGTGFVPKTGLFDG 147

Query: 999  --RIEKERPVFYG------HQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLKG 844
              +I KE     G       +W  S G++E   +   GV       SS V+ + D  +  
Sbjct: 148  SGQIRKESNAKMGLSNGSGGRWKVSDGVIEILDNSDDGVVTDVQLQSSSVVTVSDGDVNL 207

Query: 843  LP----------LRLEPNKWCK--------PPHGDLLDSARKRDPILQELAFQIQLHEKR 718
            L           L L  +K+          P +  LLDSA KR+  L+ L F IQ  EK 
Sbjct: 208  LKVENAEKMMDSLSLSNSKFDDSSSSASFVPSYKKLLDSAEKRNDNLKSLQFHIQYTEKL 267

Query: 717  LE----MSWSRXXXXXXXXXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNID 550
            LE    +   +          + E F+ L  E+++E++ A +  +SR K+LV+HE SNID
Sbjct: 268  LETYKLLRPQKKDEQYVKEDVIIEPFVQLDKEEKAEVSHALSNYSSRSKVLVTHENSNID 327

Query: 549  ITGEILQCLRPGAWLNNEVINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYN 370
            ITGEILQCLRP AWLN+EVINVYLELLKERE REP +FL+CHF NTFFYKKLI G GGYN
Sbjct: 328  ITGEILQCLRPRAWLNDEVINVYLELLKEREKREPKRFLRCHFFNTFFYKKLISGKGGYN 387

Query: 369  FQAVRRWTTQRKLGYCLFDCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRDDK 199
            +Q+V+RWT+QRKLGYCLF+CDKIFVPIH+EVHWCLAVINKKD+KFQYLDSLGGRD++
Sbjct: 388  YQSVKRWTSQRKLGYCLFECDKIFVPIHQEVHWCLAVINKKDEKFQYLDSLGGRDNQ 444



 Score = 91.3 bits (225), Expect(2) = e-100
 Identities = 40/57 (70%), Positives = 47/57 (82%)
 Frame = -2

Query: 172 YYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           Y++DEV +K G  IDVSSW+ EFV DLPEQENG+DCG+FM+KYADFYSR  GL F Q
Sbjct: 452 YFIDEVKDKNGKDIDVSSWKQEFVVDLPEQENGFDCGMFMLKYADFYSRDIGLCFNQ 508


>XP_009773778.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Nicotiana
            sylvestris] XP_016461419.1 PREDICTED:
            ubiquitin-like-specific protease ESD4 [Nicotiana tabacum]
          Length = 503

 Score =  304 bits (778), Expect(2) = e-100
 Identities = 190/434 (43%), Positives = 251/434 (57%), Gaps = 20/434 (4%)
 Frame = -3

Query: 1446 SNFDHISKKRRVSVSSDNTSTRSRCSVYRFNLYPEAKGELPRAVHAPVKNNRFSFSKRGL 1267
            SNFDHISKK ++S+    T    + +V RF +YP+    + R VHAP +++RF    +G+
Sbjct: 27   SNFDHISKKPKLSIYQ--TLNPPKSTVQRFFMYPDPINPIRRQVHAPCRHSRFG--SKGI 82

Query: 1266 FGSGKVSSADKMLNSLSNTFDILKSKYAKISREALGVIRHGVFRDVEDEVRVGVPNWRDK 1087
                  +SA +  N +S  +D  K K A  S   L  ++  +F D               
Sbjct: 83   ----SANSAPETGNFVSRVYDEAK-KSAFYSLRCLKKVKEVIFID--------------- 122

Query: 1086 EKGKEVIVLDDEATEEDVTGGSSIE-EVDMRIEK---ERPVFYGHQWNTSTGIVE--NAV 925
                     DD+A + D+  G   + +V   +E    E+  F G +W  + G +E  N V
Sbjct: 123  ---------DDDAKKGDLEAGVCKQSDVFCNLEGGVCEKSDFLGQKWKQTNGPLEILNGV 173

Query: 924  DGKRGV--------GNCKASMSSPVINMEDLSLKGLPLRLEPNK---WCKPPHGDLLDSA 778
            DG   +        GN K      +++          L L P     +  P +  LL S 
Sbjct: 174  DGHDSLVVTTGLDDGNLKEESVGKMLDT---------LALNPTSDSAFYVPLYKKLLGSV 224

Query: 777  RKRDPILQELAFQIQLHEKRLE---MSWSRXXXXXXXXXXVREAFIPLSAEDRSELARAF 607
             K +  L+ L FQI  +EK LE   +   +          + E F+PL+ E+  E++RA 
Sbjct: 225  GKINDKLKRLQFQIGYNEKCLETYSLLRPQKKEEQVKEDVISEPFVPLTEEEEVEVSRAL 284

Query: 606  AKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNEVINVYLELLKERENREPHKFLKC 427
            +K+N RR++LV+H+ SNIDITGEILQCLRP  WLN+EVINVYLELLKERE R+P KFLKC
Sbjct: 285  SKSN-RRRVLVTHQNSNIDITGEILQCLRPREWLNDEVINVYLELLKEREKRDPQKFLKC 343

Query: 426  HFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLFDCDKIFVPIHKEVHWCLAVINKK 247
            HF NTFFYKKLIGG GGYN+Q+VRRWT+QRKL YCL +CDKIFVPIHKE+HWCLAVINKK
Sbjct: 344  HFFNTFFYKKLIGGRGGYNYQSVRRWTSQRKLDYCLLECDKIFVPIHKEIHWCLAVINKK 403

Query: 246  DQKFQYLDSLGGRD 205
            D+KFQYLDSL GRD
Sbjct: 404  DKKFQYLDSLRGRD 417



 Score = 92.4 bits (228), Expect(2) = e-100
 Identities = 43/59 (72%), Positives = 48/59 (81%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K G  IDVSSW+ EFVEDLPEQ+NG+DCGVFMIK ADFYSR  GL F Q
Sbjct: 425 ARYFVDEVKDKNGKDIDVSSWKQEFVEDLPEQKNGFDCGVFMIKNADFYSRDIGLCFNQ 483


>XP_006349816.1 PREDICTED: ubiquitin-like-specific protease ESD4 [Solanum tuberosum]
          Length = 523

 Score =  301 bits (771), Expect(2) = e-100
 Identities = 199/457 (43%), Positives = 256/457 (56%), Gaps = 20/457 (4%)
 Frame = -3

Query: 1515 MGVLISNRNRYEAHFRGSLCLDLSNFDHISKKRRVSVSSDNTSTRSRCSVYRFNLYPEAK 1336
            MG L SNR R    F  +    LSN   +SKK ++S  +   S++S  +V RF  YP+  
Sbjct: 1    MGALTSNRKRGNDFFSSNCKTPLSN---LSKKLKLSALNPPLSSKS--TVQRFFKYPDPI 55

Query: 1335 GELPRAVHAPVKNNRFSF--SKRGLFGSGKVS--SADKMLNSLSNTFDILKSKYAKISRE 1168
              + R VHAP +  RF    +K        VS  S  +M N LS   +  K       R+
Sbjct: 56   NPIRREVHAPCRKLRFGSKQTKSNTVNYKGVSEYSGSEMGNCLSRKHEETKRSDFDGLRK 115

Query: 1167 ALGVI-------RHGVFRDVEDEVRVGVPNWRDKEKGKEVIV-----LDDEATEEDVTGG 1024
             + VI       + GV  D   E    V  W  K+   ++       L+D + EE    G
Sbjct: 116  DIEVIDIDGDDAKEGVSEDSSIEE---VEAWGCKKSDLDIAAAAANGLEDSSIEESERWG 172

Query: 1023 SSIEEV-DMRIEKERPVFYGHQWNTSTGIVENAVDGKRGVGNCKASMSSPVINMEDLSLK 847
                ++    ++ +  +  G+    S+ +     DG     N K      +I  E L++ 
Sbjct: 173  CEKSDLLGQEVDDDMKILDGYDGKDSSVLTTGLDDG-----NLKEESVGKMI--ESLAMN 225

Query: 846  GLPLRLEPNKWCKPPHGDLLDSARKRDPILQELAFQIQLHEKRLEMSW---SRXXXXXXX 676
              P     + +  P +  LL S  K    L+ L FQI L+EK LE +     +       
Sbjct: 226  PTP----DSYFYVPLYKKLLGSVDKISDKLKRLQFQIGLNEKSLETNRLLRPQKKEEQVI 281

Query: 675  XXXVREAFIPLSAEDRSELARAFAKANSRRKILVSHEKSNIDITGEILQCLRPGAWLNNE 496
               + E F+PL+ E+  E+A A +K+N RRK+LV+H+ SNIDITGEILQCLRPGAWLN+E
Sbjct: 282  EDAISEPFVPLTEEEHDEVACALSKSN-RRKVLVTHKSSNIDITGEILQCLRPGAWLNDE 340

Query: 495  VINVYLELLKERENREPHKFLKCHFLNTFFYKKLIGGNGGYNFQAVRRWTTQRKLGYCLF 316
            VINVYLELLKERE REP KFLKCHF NTFFYKKLI G GGYN+Q+VRRWT+QRKLGY L 
Sbjct: 341  VINVYLELLKEREKREPQKFLKCHFFNTFFYKKLISGTGGYNYQSVRRWTSQRKLGYSLL 400

Query: 315  DCDKIFVPIHKEVHWCLAVINKKDQKFQYLDSLGGRD 205
            +CDKIFVPIHKE+HWCLAVINKKDQKFQYLDSL GRD
Sbjct: 401  ECDKIFVPIHKEIHWCLAVINKKDQKFQYLDSLRGRD 437



 Score = 94.4 bits (233), Expect(2) = e-100
 Identities = 44/59 (74%), Positives = 49/59 (83%)
 Frame = -2

Query: 178 AMYYVDEVNEKTGNAIDVSSWQHEFVEDLPEQENGYDCGVFMIKYADFYSRGGGLHFKQ 2
           A Y+VDEV +K+G  IDVSSW+ EFVEDLPEQ+NGYDCGVFMIK ADFYSR  GL F Q
Sbjct: 445 ASYFVDEVKDKSGKHIDVSSWKQEFVEDLPEQKNGYDCGVFMIKNADFYSRDIGLCFNQ 503