BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00017702
         (548 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KDO43141.1 hypothetical protein CISIN_1g0248531mg, partial [Citr...    89   7e-19
XP_019223412.1 PREDICTED: uncharacterized protein LOC109205186 [...    91   8e-19
XP_015056897.1 PREDICTED: uncharacterized protein LOC107003144 i...    91   8e-19
XP_015056896.1 PREDICTED: uncharacterized protein LOC107003144 i...    91   8e-19
XP_009794078.1 PREDICTED: uncharacterized protein LOC104240878 i...    89   3e-18
XP_009794077.1 PREDICTED: uncharacterized protein LOC104240878 i...    89   4e-18
XP_006441612.1 hypothetical protein CICLE_v10021221mg [Citrus cl...    88   2e-17
XP_004250651.1 PREDICTED: uncharacterized protein LOC101253651 i...    87   2e-17
XP_010312982.1 PREDICTED: uncharacterized protein LOC101253651 i...    87   2e-17
XP_006478269.1 PREDICTED: uncharacterized protein LOC102625451 i...    87   5e-17
XP_006478268.1 PREDICTED: uncharacterized protein LOC102625451 i...    87   5e-17
KJB81204.1 hypothetical protein B456_013G136900 [Gossypium raimo...    85   1e-16
KJB81208.1 hypothetical protein B456_013G136900 [Gossypium raimo...    85   1e-16
XP_010100101.1 hypothetical protein L484_014291 [Morus notabilis...    82   6e-16
XP_010665313.1 PREDICTED: uncharacterized protein LOC100241456 i...    83   8e-16
XP_006339352.1 PREDICTED: uncharacterized protein LOC102602767 i...    82   9e-16
XP_006339351.1 PREDICTED: uncharacterized protein LOC102602767 i...    82   9e-16
XP_017617430.1 PREDICTED: uncharacterized protein LOC108461944 i...    82   1e-15
XP_016675792.1 PREDICTED: uncharacterized protein LOC107895087 i...    82   1e-15
KCW69519.1 hypothetical protein EUGRSUZ_F02959 [Eucalyptus grandis]    82   1e-15

>KDO43141.1 hypothetical protein CISIN_1g0248531mg, partial [Citrus sinensis]
          Length = 206

 Score = 89.4 bits (220), Expect = 7e-19
 Identities = 51/108 (47%), Positives = 67/108 (62%), Gaps = 5/108 (4%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLV+ PLIAFCIRCM+ + H ++ G   HR  N    + HP   S RWKSAL D
Sbjct: 83  AKTTGYFLVVVPLIAFCIRCMIGAFHTRIAGGSGHRVANGSKGR-HPGSKSVRWKSALRD 141

Query: 371 FDPDTRDSASRPDSDDFADGDHQ----LHDEDSAKAYMELERDYQKFL 240
              +  DS SRP ++D  +   Q    +  E+S+ AY +LE DY+KFL
Sbjct: 142 IIEEDLDSGSRPRTNDPVEEQDQAQPLISFEESSHAYRKLEDDYEKFL 189


>XP_019223412.1 PREDICTED: uncharacterized protein LOC109205186 [Nicotiana
           attenuata] OIT34070.1 hypothetical protein A4A49_09274
           [Nicotiana attenuata]
          Length = 267

 Score = 90.5 bits (223), Expect = 8e-19
 Identities = 50/104 (48%), Positives = 63/104 (60%), Gaps = 2/104 (1%)
 Frame = -1

Query: 545 RTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGDF 369
           +TTGYFLVL PLIAFCIRC++ + HH+V         N L + +     S RWK AL D 
Sbjct: 155 KTTGYFLVLVPLIAFCIRCIVTAFHHRV-------ARNKLQD-VRQASGSMRWKLALRDL 206

Query: 368 -DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
            DPD   + SRPD D+  D     H ED ++AY +L+ DYQKFL
Sbjct: 207 SDPDASYTDSRPDIDNVTDDQDNFHSEDHSRAYAKLDHDYQKFL 250


>XP_015056897.1 PREDICTED: uncharacterized protein LOC107003144 isoform X2 [Solanum
           pennellii]
          Length = 267

 Score = 90.5 bits (223), Expect = 8e-19
 Identities = 51/105 (48%), Positives = 66/105 (62%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+GYFLVL PLI FCIRC+M + HH+V         N L + +H    S RWKSAL D
Sbjct: 154 AKTSGYFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQD-VHQTSGSMRWKSALRD 205

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP++D+  D    L  E+ ++AY +L+ DYQKFL
Sbjct: 206 LTDPDALYSDSRPETDNVTDDREDLQSEELSRAYAKLDGDYQKFL 250


>XP_015056896.1 PREDICTED: uncharacterized protein LOC107003144 isoform X1 [Solanum
           pennellii]
          Length = 268

 Score = 90.5 bits (223), Expect = 8e-19
 Identities = 51/105 (48%), Positives = 66/105 (62%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+GYFLVL PLI FCIRC+M + HH+V         N L + +H    S RWKSAL D
Sbjct: 155 AKTSGYFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQD-VHQTSGSMRWKSALRD 206

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP++D+  D    L  E+ ++AY +L+ DYQKFL
Sbjct: 207 LTDPDALYSDSRPETDNVTDDREDLQSEELSRAYAKLDGDYQKFL 251


>XP_009794078.1 PREDICTED: uncharacterized protein LOC104240878 isoform X2
           [Nicotiana sylvestris] XP_016456222.1 PREDICTED:
           uncharacterized protein LOC107780201 isoform X2
           [Nicotiana tabacum]
          Length = 268

 Score = 89.0 bits (219), Expect = 3e-18
 Identities = 48/105 (45%), Positives = 63/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLVL PLIAFCIRC+M++  H       HR   +  + +     S RWK AL D
Sbjct: 154 AKTTGYFLVLVPLIAFCIRCIMMNAFH-------HRVARNKLQDVRQASGSMRWKLALRD 206

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   + SRP+ ++  D     H ED ++AY +L+ DYQKFL
Sbjct: 207 LSDPDASYTDSRPEIENVTDDQDNFHSEDHSRAYAKLDHDYQKFL 251


>XP_009794077.1 PREDICTED: uncharacterized protein LOC104240878 isoform X1
           [Nicotiana sylvestris] XP_016456221.1 PREDICTED:
           uncharacterized protein LOC107780201 isoform X1
           [Nicotiana tabacum]
          Length = 275

 Score = 89.0 bits (219), Expect = 4e-18
 Identities = 48/105 (45%), Positives = 63/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLVL PLIAFCIRC+M++  H       HR   +  + +     S RWK AL D
Sbjct: 161 AKTTGYFLVLVPLIAFCIRCIMMNAFH-------HRVARNKLQDVRQASGSMRWKLALRD 213

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   + SRP+ ++  D     H ED ++AY +L+ DYQKFL
Sbjct: 214 LSDPDASYTDSRPEIENVTDDQDNFHSEDHSRAYAKLDHDYQKFL 258


>XP_006441612.1 hypothetical protein CICLE_v10021221mg [Citrus clementina]
           ESR54852.1 hypothetical protein CICLE_v10021221mg
           [Citrus clementina]
          Length = 318

 Score = 87.8 bits (216), Expect = 2e-17
 Identities = 50/108 (46%), Positives = 66/108 (61%), Gaps = 5/108 (4%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLV+ PLIAFCIRCM+ + H ++ G   HR  N    + HP   S RWKSAL D
Sbjct: 195 AKTTGYFLVVVPLIAFCIRCMIGAFHTRIAGGSGHRVANGSKGR-HPGSKSVRWKSALRD 253

Query: 371 FDPDTRDSASRPDSDDFADGDHQ----LHDEDSAKAYMELERDYQKFL 240
              +  DS SRP ++D  +   Q    +  E+S+  Y +LE DY+KFL
Sbjct: 254 IIEEDLDSGSRPRTNDPVEEQDQAQPLISFEESSHVYRKLEDDYEKFL 301


>XP_004250651.1 PREDICTED: uncharacterized protein LOC101253651 isoform X2 [Solanum
           lycopersicum]
          Length = 267

 Score = 86.7 bits (213), Expect = 2e-17
 Identities = 50/105 (47%), Positives = 64/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+G+FLVL PLI FCIRC+M + HH+V         N L +  H    S RWKSAL D
Sbjct: 154 AKTSGHFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQDA-HQTSGSMRWKSALRD 205

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP++D+  D    L  E+ + AY +L+ DYQKFL
Sbjct: 206 LTDPDALYSDSRPETDNVTDDREHLQSEELSHAYAKLDGDYQKFL 250


>XP_010312982.1 PREDICTED: uncharacterized protein LOC101253651 isoform X1 [Solanum
           lycopersicum]
          Length = 268

 Score = 86.7 bits (213), Expect = 2e-17
 Identities = 50/105 (47%), Positives = 64/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+G+FLVL PLI FCIRC+M + HH+V         N L +  H    S RWKSAL D
Sbjct: 155 AKTSGHFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQDA-HQTSGSMRWKSALRD 206

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP++D+  D    L  E+ + AY +L+ DYQKFL
Sbjct: 207 LTDPDALYSDSRPETDNVTDDREHLQSEELSHAYAKLDGDYQKFL 251


>XP_006478269.1 PREDICTED: uncharacterized protein LOC102625451 isoform X2 [Citrus
           sinensis]
          Length = 317

 Score = 86.7 bits (213), Expect = 5e-17
 Identities = 49/108 (45%), Positives = 66/108 (61%), Gaps = 5/108 (4%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLV+ PLIAFCIRCM+ + H ++ G   H+  N    + HP   + RWKSAL D
Sbjct: 194 AKTTGYFLVVVPLIAFCIRCMIGAFHTRIAGGSGHQVANGSKGR-HPGSKTVRWKSALRD 252

Query: 371 FDPDTRDSASRPDSDDFADGDHQLHD----EDSAKAYMELERDYQKFL 240
              +  DS SRP ++D  +   Q       E+S+ AY +LE DY+KFL
Sbjct: 253 IIEEDLDSGSRPRTNDPVEEQDQAQPLVSFEESSHAYRKLEDDYEKFL 300


>XP_006478268.1 PREDICTED: uncharacterized protein LOC102625451 isoform X1 [Citrus
           sinensis]
          Length = 318

 Score = 86.7 bits (213), Expect = 5e-17
 Identities = 49/108 (45%), Positives = 66/108 (61%), Gaps = 5/108 (4%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLV+ PLIAFCIRCM+ + H ++ G   H+  N    + HP   + RWKSAL D
Sbjct: 195 AKTTGYFLVVVPLIAFCIRCMIGAFHTRIAGGSGHQVANGSKGR-HPGSKTVRWKSALRD 253

Query: 371 FDPDTRDSASRPDSDDFADGDHQLHD----EDSAKAYMELERDYQKFL 240
              +  DS SRP ++D  +   Q       E+S+ AY +LE DY+KFL
Sbjct: 254 IIEEDLDSGSRPRTNDPVEEQDQAQPLVSFEESSHAYRKLEDDYEKFL 301


>KJB81204.1 hypothetical protein B456_013G136900 [Gossypium raimondii]
          Length = 273

 Score = 85.1 bits (209), Expect = 1e-16
 Identities = 48/105 (45%), Positives = 68/105 (64%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A++TGYFLVL PLIAFCI C++V+LH +V  + KH+   D  +  HP    +RWKSAL D
Sbjct: 152 AKSTGYFLVLVPLIAFCIGCIIVTLHTRVSRSIKHQAA-DESQAHHPGAKGRRWKSALSD 210

Query: 371 -FDPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             + D  DS    DS+  ++ +  + +E+++K Y  LE DYQKFL
Sbjct: 211 SVEGDVFDSELGLDSNSTSEDEANIQNEEASKDYGRLEHDYQKFL 255


>KJB81208.1 hypothetical protein B456_013G136900 [Gossypium raimondii]
          Length = 274

 Score = 85.1 bits (209), Expect = 1e-16
 Identities = 48/105 (45%), Positives = 68/105 (64%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A++TGYFLVL PLIAFCI C++V+LH +V  + KH+   D  +  HP    +RWKSAL D
Sbjct: 153 AKSTGYFLVLVPLIAFCIGCIIVTLHTRVSRSIKHQAA-DESQAHHPGAKGRRWKSALSD 211

Query: 371 -FDPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             + D  DS    DS+  ++ +  + +E+++K Y  LE DYQKFL
Sbjct: 212 SVEGDVFDSELGLDSNSTSEDEANIQNEEASKDYGRLEHDYQKFL 256


>XP_010100101.1 hypothetical protein L484_014291 [Morus notabilis] EXB81484.1
           hypothetical protein L484_014291 [Morus notabilis]
          Length = 223

 Score = 82.0 bits (201), Expect = 6e-16
 Identities = 45/105 (42%), Positives = 67/105 (63%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           ART G FLVL  L+AFCIRC++ + H++  G  +H+  N+   K H      RW+SALGD
Sbjct: 103 ARTVGGFLVLVSLVAFCIRCIVGAFHNRASGEFRHKNVNESDVK-HLRSQGGRWRSALGD 161

Query: 371 -FDPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
            ++P+  D  S PDS + ++   Q+  E+++ AY +LE DYQ+FL
Sbjct: 162 AWEPERTDGDSIPDSRNHSEDQPQISFEEASHAYRKLEHDYQEFL 206


>XP_010665313.1 PREDICTED: uncharacterized protein LOC100241456 isoform X1 [Vitis
           vinifera]
          Length = 279

 Score = 82.8 bits (203), Expect = 8e-16
 Identities = 48/105 (45%), Positives = 67/105 (63%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+ TGYFLVL PLIAFCIRC++ +  ++V G  +H+  N+     H    S RWKSAL D
Sbjct: 159 AKATGYFLVLVPLIAFCIRCIIGAFQNRVVGDLRHQAVNESEVDYHG-SKSVRWKSALDD 217

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             +PDT D+  +PDS + ++   Q   E+ + AY +LE DYQ+FL
Sbjct: 218 IREPDTLDTGLQPDSINPSEVQTQGSAEEVSHAYGKLEHDYQQFL 262


>XP_006339352.1 PREDICTED: uncharacterized protein LOC102602767 isoform X2 [Solanum
           tuberosum]
          Length = 267

 Score = 82.4 bits (202), Expect = 9e-16
 Identities = 48/105 (45%), Positives = 63/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+GYFLVL PLI FCIRC+M + HH+V         N L + +     S RWK AL D
Sbjct: 154 AKTSGYFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQD-VRQASGSMRWKLALRD 205

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP+ ++  D    L  E+ ++AY +L+ DYQKFL
Sbjct: 206 LSDPDALYSDSRPEIENVTDDREHLQSEELSRAYAKLDGDYQKFL 250


>XP_006339351.1 PREDICTED: uncharacterized protein LOC102602767 isoform X1 [Solanum
           tuberosum]
          Length = 268

 Score = 82.4 bits (202), Expect = 9e-16
 Identities = 48/105 (45%), Positives = 63/105 (60%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+T+GYFLVL PLI FCIRC+M + HH+V         N L + +     S RWK AL D
Sbjct: 155 AKTSGYFLVLVPLIGFCIRCIMNAFHHRV-------ARNKLQD-VRQASGSMRWKLALRD 206

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             DPD   S SRP+ ++  D    L  E+ ++AY +L+ DYQKFL
Sbjct: 207 LSDPDALYSDSRPEIENVTDDREHLQSEELSRAYAKLDGDYQKFL 251


>XP_017617430.1 PREDICTED: uncharacterized protein LOC108461944 isoform X3
           [Gossypium arboreum]
          Length = 274

 Score = 82.0 bits (201), Expect = 1e-15
 Identities = 46/105 (43%), Positives = 67/105 (63%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A++TGYFLVL PLIAFCI C++ +LH +V  + KH+   D  +  HP    +RWKSAL D
Sbjct: 153 AKSTGYFLVLVPLIAFCIGCIISTLHTRVSRSIKHQAA-DKSQAHHPGAKGRRWKSALSD 211

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             + D  DS    DS+  ++ +  + +E++++ Y  LE DYQKFL
Sbjct: 212 LVEGDVFDSELGLDSNSTSEDEANIQNEEASEDYGRLEHDYQKFL 256


>XP_016675792.1 PREDICTED: uncharacterized protein LOC107895087 isoform X3
           [Gossypium hirsutum]
          Length = 274

 Score = 82.0 bits (201), Expect = 1e-15
 Identities = 46/105 (43%), Positives = 67/105 (63%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A++TGYFLVL PLIAFCI C++ +LH +V  + KH+   D  +  HP    +RWKSAL D
Sbjct: 153 AKSTGYFLVLVPLIAFCIGCIISTLHTRVSRSIKHQAA-DKSQAHHPGAKGRRWKSALSD 211

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             + D  DS    DS+  ++ +  + +E++++ Y  LE DYQKFL
Sbjct: 212 LVEGDVFDSELGLDSNSTSEDEANIQNEEASEDYGRLEHDYQKFL 256


>KCW69519.1 hypothetical protein EUGRSUZ_F02959 [Eucalyptus grandis]
          Length = 300

 Score = 82.4 bits (202), Expect = 1e-15
 Identities = 51/105 (48%), Positives = 66/105 (62%), Gaps = 2/105 (1%)
 Frame = -1

Query: 548 ARTTGYFLVL-PLIAFCIRCMMVSLHHKVFGAPKHRTENDLPEKLHPIPNSKRWKSALGD 372
           A+TTGYFLVL PLIAFCIRC+   L  +V G  + R +N  P++ H +P SKRWKSAL D
Sbjct: 174 AKTTGYFLVLVPLIAFCIRCITSVLCTRVAGYLR-REDNIEPKQHHQVPKSKRWKSALLD 232

Query: 371 F-DPDTRDSASRPDSDDFADGDHQLHDEDSAKAYMELERDYQKFL 240
             + D  D  S+ D    ++   Q    D+ +AY +LE DYQKFL
Sbjct: 233 AKELDVSDPESKSDIGCPSEDQAQSSSGDTFQAYGKLEEDYQKFL 277