BLASTX nr result
ID: Lithospermum23_contig00017388
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00017388
(334 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_010558043.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 155 5e-43
XP_017240639.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 150 8e-41
CDY56848.1 BnaCnng31250D [Brassica napus] 148 8e-41
XP_015072943.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 149 9e-41
XP_018462125.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 149 9e-41
XP_015876833.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 149 9e-41
XP_018462124.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 149 1e-40
XP_002315213.1 hypothetical protein POPTR_0010s20880g [Populus t... 149 1e-40
XP_006346708.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 149 1e-40
KVI04917.1 Phospholipid/glycerol acyltransferase [Cynara cardunc... 149 2e-40
EYU19928.1 hypothetical protein MIMGU_mgv1a018205mg [Erythranthe... 148 2e-40
XP_012858231.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 148 3e-40
XP_002312108.1 hypothetical protein POPTR_0008s05840g [Populus t... 148 3e-40
XP_002531580.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 148 3e-40
XP_004236735.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 148 3e-40
XP_013640165.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 148 4e-40
XP_006407448.1 hypothetical protein EUTSA_v10020574mg [Eutrema s... 148 4e-40
XP_013584222.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 147 5e-40
XP_016574533.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 146 1e-39
XP_018488624.1 PREDICTED: glycerol-3-phosphate acyltransferase 5... 146 1e-39
>XP_010558043.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Tarenaya
hassleriana]
Length = 502
Score = 155 bits (393), Expect = 5e-43
Identities = 73/108 (67%), Positives = 85/108 (78%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
+RP+PVIFHDGRL KRPTP+ A A++RI IGF LP+WA+PY+S IFGG
Sbjct: 216 LRPKPVIFHDGRLVKRPTPVTALLILLWIPFGIVLATVRILIGFVLPLWAIPYVSSIFGG 275
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
+V+VKG PPS P+ GNSGVLFVCTHR+LMDPVVLS VL RRIPAVTYS
Sbjct: 276 RVIVKGKPPSPPAGGNSGVLFVCTHRTLMDPVVLSYVLGRRIPAVTYS 323
>XP_017240639.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Daucus
carota subsp. sativus] KZN02965.1 hypothetical protein
DCAR_011721 [Daucus carota subsp. sativus]
Length = 504
Score = 150 bits (378), Expect = 8e-41
Identities = 73/110 (66%), Positives = 81/110 (73%)
Frame = +2
Query: 5 YDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIF 184
+ IRP PVIFHDGRL KRPTP + A IRI +G LPMWA+PY+S +F
Sbjct: 209 HHIRPLPVIFHDGRLVKRPTPFTSLLIILWIPIGIMLAIIRILMGMVLPMWAIPYMSQLF 268
Query: 185 GGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
GG VVVKG PP PS NSGVLFVCTHR+LMDPVVLSTVL+RRIPAVTYS
Sbjct: 269 GGSVVVKGKPPPPPSRSNSGVLFVCTHRTLMDPVVLSTVLRRRIPAVTYS 318
>CDY56848.1 BnaCnng31250D [Brassica napus]
Length = 407
Score = 148 bits (373), Expect = 8e-41
Identities = 71/111 (63%), Positives = 82/111 (73%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q ++RP PVIFHDGRL KRPTP A A+IRIF+G LP+WA PY+S I
Sbjct: 149 QLELRPLPVIFHDGRLVKRPTPATALLILIWIPFGIILAAIRIFLGSILPLWATPYVSKI 208
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+++VKG PP PS G+SGVLFVCTHR+LMDPVVLS VL R IPAVTYS
Sbjct: 209 FGGQIIVKGKPPQAPSPGHSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYS 259
>XP_015072943.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Solanum
pennellii]
Length = 491
Score = 149 bits (377), Expect = 9e-41
Identities = 71/108 (65%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
I+P PVIFHDGRL KRPTP +A A+IRI IG LP+W +PY++P+FGG
Sbjct: 202 IKPLPVIFHDGRLVKRPTPSIALLILLWIPFGIILATIRIIIGLMLPLWIVPYLAPLFGG 261
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV+VKG PP S NSGVLFVCTHR+L+DPVVLSTVLQRRIPAVTYS
Sbjct: 262 KVIVKGKPPPPASTTNSGVLFVCTHRTLLDPVVLSTVLQRRIPAVTYS 309
>XP_018462125.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like isoform X2
[Raphanus sativus]
Length = 492
Score = 149 bits (377), Expect = 9e-41
Identities = 71/111 (63%), Positives = 83/111 (74%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q+++RP PVIFHDGRL KRPTP A A+IRIF+G LP+WA PY+S I
Sbjct: 212 QFELRPLPVIFHDGRLVKRPTPPTALLILTWIPLGIILAAIRIFLGSVLPLWATPYVSKI 271
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+V+VKG PP P+ GNSGVLFVCTHR+LMDPVVLS VL R +PAVTYS
Sbjct: 272 FGGQVIVKGKPPQAPTAGNSGVLFVCTHRTLMDPVVLSYVLGRSVPAVTYS 322
>XP_015876833.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Ziziphus
jujuba]
Length = 496
Score = 149 bits (377), Expect = 9e-41
Identities = 72/108 (66%), Positives = 80/108 (74%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
+RP PVIFHDGRL KRPTP A A +RI IG LPMWA+PY+S +FGG
Sbjct: 210 LRPLPVIFHDGRLVKRPTPSTALLILLWIPLGIILAFVRIIIGLILPMWAIPYVSRLFGG 269
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
V+VKG PP SNGN GVLFVCTHR+LMDPVVLSTVL+RRIPAVTYS
Sbjct: 270 NVIVKGKPPPPASNGNPGVLFVCTHRTLMDPVVLSTVLRRRIPAVTYS 317
>XP_018462124.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like isoform X1
[Raphanus sativus]
Length = 501
Score = 149 bits (377), Expect = 1e-40
Identities = 71/111 (63%), Positives = 83/111 (74%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q+++RP PVIFHDGRL KRPTP A A+IRIF+G LP+WA PY+S I
Sbjct: 212 QFELRPLPVIFHDGRLVKRPTPPTALLILTWIPLGIILAAIRIFLGSVLPLWATPYVSKI 271
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+V+VKG PP P+ GNSGVLFVCTHR+LMDPVVLS VL R +PAVTYS
Sbjct: 272 FGGQVIVKGKPPQAPTAGNSGVLFVCTHRTLMDPVVLSYVLGRSVPAVTYS 322
>XP_002315213.1 hypothetical protein POPTR_0010s20880g [Populus trichocarpa]
EEF01384.1 hypothetical protein POPTR_0010s20880g
[Populus trichocarpa]
Length = 488
Score = 149 bits (376), Expect = 1e-40
Identities = 74/114 (64%), Positives = 83/114 (72%), Gaps = 3/114 (2%)
Frame = +2
Query: 2 QYD---IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYI 172
QYD +RP PVIFHDGRL KRPTP A A+IRI +G LPMWA PY+
Sbjct: 196 QYDHQLVRPLPVIFHDGRLVKRPTPSTALLIILWMPLGIILATIRILVGVMLPMWAKPYL 255
Query: 173 SPIFGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
S + GGKV+VKG PP S GNSGVLFVCTHR+LMDPVVLSTVL+R+IPAVTYS
Sbjct: 256 SRVLGGKVIVKGKPPPPASGGNSGVLFVCTHRTLMDPVVLSTVLRRKIPAVTYS 309
>XP_006346708.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Solanum
tuberosum]
Length = 488
Score = 149 bits (376), Expect = 1e-40
Identities = 71/108 (65%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
I+P PVIFHDGRL KRPTP +A A+IRI IG LP+W +PY++P+FGG
Sbjct: 202 IKPLPVIFHDGRLVKRPTPSIALLILLWIPFGVILATIRIIIGLVLPLWIVPYLAPLFGG 261
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV+VKG PP S NSGVLFVCTHR+L+DPVVLSTVLQRRIPAVTYS
Sbjct: 262 KVIVKGKPPPPASTTNSGVLFVCTHRTLLDPVVLSTVLQRRIPAVTYS 309
>KVI04917.1 Phospholipid/glycerol acyltransferase [Cynara cardunculus var.
scolymus]
Length = 510
Score = 149 bits (376), Expect = 2e-40
Identities = 74/109 (67%), Positives = 81/109 (74%)
Frame = +2
Query: 8 DIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFG 187
D+RP PVIFHDGRL KRPTP A A IR+ IG LPMWA+PY++ IFG
Sbjct: 215 DMRPMPVIFHDGRLVKRPTPSTALLILLWIPLGVILAIIRMTIGVLLPMWAIPYMASIFG 274
Query: 188 GKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
GKVVVKG PP PS N+GVLFVCTHR+LMDPVVLSTVLQR IPAVTYS
Sbjct: 275 GKVVVKGVPPPPPSGSNAGVLFVCTHRTLMDPVVLSTVLQRSIPAVTYS 323
>EYU19928.1 hypothetical protein MIMGU_mgv1a018205mg [Erythranthe guttata]
Length = 469
Score = 148 bits (374), Expect = 2e-40
Identities = 72/108 (66%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
IRP PVIFHDGRLA RPTP A A+IRI +G LPMWA+P+++PIFG
Sbjct: 179 IRPLPVIFHDGRLAMRPTPAAALLILLWIPIGILLAAIRISVGIILPMWAIPHVAPIFGS 238
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
+VVVKG PP PS NSGVLFVCTHR+LMDPVVLS+VL+RRIPAVTYS
Sbjct: 239 RVVVKGTPPPPPSASNSGVLFVCTHRTLMDPVVLSSVLRRRIPAVTYS 286
>XP_012858231.1 PREDICTED: glycerol-3-phosphate acyltransferase 5 [Erythranthe
guttata]
Length = 503
Score = 148 bits (374), Expect = 3e-40
Identities = 72/108 (66%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
IRP PVIFHDGRLA RPTP A A+IRI +G LPMWA+P+++PIFG
Sbjct: 213 IRPLPVIFHDGRLAMRPTPAAALLILLWIPIGILLAAIRISVGIILPMWAIPHVAPIFGS 272
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
+VVVKG PP PS NSGVLFVCTHR+LMDPVVLS+VL+RRIPAVTYS
Sbjct: 273 RVVVKGTPPPPPSASNSGVLFVCTHRTLMDPVVLSSVLRRRIPAVTYS 320
>XP_002312108.1 hypothetical protein POPTR_0008s05840g [Populus trichocarpa]
EEE89475.1 hypothetical protein POPTR_0008s05840g
[Populus trichocarpa]
Length = 489
Score = 148 bits (373), Expect = 3e-40
Identities = 70/108 (64%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
+RP PVIFHDGRL KRPTP +A A++RI +G LPMWA PY+S + GG
Sbjct: 203 VRPLPVIFHDGRLVKRPTPSMALLIILWMPIGIVLAAVRILVGIMLPMWAKPYLSKMLGG 262
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV+VKG PP S+GNSGVLFVCTHR+LMDPVVLSTVL+R+IPAVTYS
Sbjct: 263 KVIVKGKPPPPASSGNSGVLFVCTHRTLMDPVVLSTVLRRKIPAVTYS 310
>XP_002531580.1 PREDICTED: glycerol-3-phosphate acyltransferase 5 [Ricinus
communis] EEF30816.1 ER glycerol-phosphate
acyltransferase [Ricinus communis]
Length = 490
Score = 148 bits (373), Expect = 3e-40
Identities = 71/108 (65%), Positives = 80/108 (74%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
IRP PVIFHDGRL KRPTP A A IRI +G LPMWA PY+S + GG
Sbjct: 204 IRPLPVIFHDGRLVKRPTPFTALLIILWIPLGIILALIRILVGVMLPMWAKPYLSRLLGG 263
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV+VKG PP P++GN GVLFVCTHR+LMDPVVLSTVL+R+IPAVTYS
Sbjct: 264 KVIVKGKPPPPPADGNKGVLFVCTHRTLMDPVVLSTVLKRKIPAVTYS 311
>XP_004236735.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Solanum
lycopersicum]
Length = 491
Score = 148 bits (373), Expect = 3e-40
Identities = 71/108 (65%), Positives = 82/108 (75%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
I+P PVIFHDGRL KRPTP +A A+IRI IG LP+W +PY++P+FGG
Sbjct: 202 IKPLPVIFHDGRLVKRPTPSIALLILLWIPFGIILATIRIIIGLILPLWIVPYLAPLFGG 261
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV+VKG PP S NSGVLFVCTHR+L+DPVVLSTVLQRRIPAVTYS
Sbjct: 262 KVIVKGKPPPPASITNSGVLFVCTHRTLLDPVVLSTVLQRRIPAVTYS 309
>XP_013640165.1 PREDICTED: glycerol-3-phosphate acyltransferase 5 [Brassica napus]
Length = 500
Score = 148 bits (373), Expect = 4e-40
Identities = 71/111 (63%), Positives = 82/111 (73%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q ++RP PVIFHDGRL KRPTP A A+IRIF+G LP+WA PY+S I
Sbjct: 211 QLELRPLPVIFHDGRLVKRPTPATALLILIWIPFGIILAAIRIFLGSILPLWATPYVSKI 270
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+++VKG PP PS G+SGVLFVCTHR+LMDPVVLS VL R IPAVTYS
Sbjct: 271 FGGQIIVKGKPPQAPSPGHSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYS 321
>XP_006407448.1 hypothetical protein EUTSA_v10020574mg [Eutrema salsugineum]
ESQ48901.1 hypothetical protein EUTSA_v10020574mg
[Eutrema salsugineum]
Length = 500
Score = 148 bits (373), Expect = 4e-40
Identities = 70/111 (63%), Positives = 82/111 (73%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q ++RP PVIFHDGRL KRPTP A A++RIF+G LP+WA PY+S I
Sbjct: 211 QLELRPLPVIFHDGRLVKRPTPATALLILIWIPFGIILAAVRIFLGSVLPLWATPYVSQI 270
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+++VKG PP P+ GNSGVLFVCTHR+LMDPVVLS VL R IPAVTYS
Sbjct: 271 FGGQIIVKGKPPQPPAAGNSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYS 321
>XP_013584222.1 PREDICTED: glycerol-3-phosphate acyltransferase 5 [Brassica
oleracea var. oleracea]
Length = 500
Score = 147 bits (372), Expect = 5e-40
Identities = 70/111 (63%), Positives = 82/111 (73%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q ++RP PVIFHDGRL KRPTP A A++RIF+G LP+WA PY+S I
Sbjct: 211 QLELRPLPVIFHDGRLVKRPTPATALLILIWIPFGIILAALRIFLGSILPLWATPYVSKI 270
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+++VKG PP P+ GNSGVLFVCTHR+LMDPVVLS VL R IPAVTYS
Sbjct: 271 FGGQIIVKGKPPQAPALGNSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYS 321
>XP_016574533.1 PREDICTED: glycerol-3-phosphate acyltransferase 5-like [Capsicum
annuum]
Length = 495
Score = 146 bits (369), Expect = 1e-39
Identities = 69/108 (63%), Positives = 80/108 (74%)
Frame = +2
Query: 11 IRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPIFGG 190
I+P PVIFHDGRL KRPTP +A A+IRI IG LP+W +PY++P+FGG
Sbjct: 205 IKPLPVIFHDGRLVKRPTPSIALLIILWIPFGVILATIRIVIGLMLPLWTVPYLAPLFGG 264
Query: 191 KVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
KV VKG PP S NSGV FVCTHR+L+DPVVLSTVLQRR+PAVTYS
Sbjct: 265 KVTVKGKPPPPASTTNSGVFFVCTHRTLLDPVVLSTVLQRRVPAVTYS 312
>XP_018488624.1 PREDICTED: glycerol-3-phosphate acyltransferase 5 [Raphanus
sativus]
Length = 500
Score = 146 bits (369), Expect = 1e-39
Identities = 71/111 (63%), Positives = 81/111 (72%)
Frame = +2
Query: 2 QYDIRPQPVIFHDGRLAKRPTPLVAXXXXXXXXXXXXXASIRIFIGFSLPMWALPYISPI 181
Q ++RP PVIFHDGRL KRPTP A A+IRIF+G LP+WA PY+S I
Sbjct: 211 QLELRPLPVIFHDGRLVKRPTPATALLILIWIPFGIILAAIRIFLGSILPLWATPYVSKI 270
Query: 182 FGGKVVVKGNPPSTPSNGNSGVLFVCTHRSLMDPVVLSTVLQRRIPAVTYS 334
FGG+++VKG P PS GNSGVLFVCTHR+LMDPVVLS VL R IPAVTYS
Sbjct: 271 FGGQIIVKGKPTQPPSPGNSGVLFVCTHRTLMDPVVLSYVLGRSIPAVTYS 321