BLASTX nr result
ID: Lithospermum23_contig00017252
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00017252
(1829 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
GAV70450.1 zf-RVT domain-containing protein [Cephalotus follicul... 100 1e-26
GAV68715.1 zf-RVT domain-containing protein [Cephalotus follicul... 101 2e-26
GAV61706.1 zf-RVT domain-containing protein [Cephalotus follicul... 102 3e-26
GAV66493.1 zf-RVT domain-containing protein [Cephalotus follicul... 101 2e-25
GAV92989.1 zf-RVT domain-containing protein, partial [Cephalotus... 98 2e-25
GAV75138.1 zf-RVT domain-containing protein [Cephalotus follicul... 114 3e-25
GAV74389.1 zf-RVT domain-containing protein [Cephalotus follicul... 100 4e-25
GAV77512.1 zf-RVT domain-containing protein [Cephalotus follicul... 100 4e-25
XP_018473660.1 PREDICTED: uncharacterized protein LOC108844871 [... 86 5e-25
GAV93027.1 zf-RVT domain-containing protein, partial [Cephalotus... 95 5e-25
XP_013588944.1 PREDICTED: uncharacterized protein LOC106297205 [... 82 8e-25
GAV91388.1 zf-RVT domain-containing protein [Cephalotus follicul... 101 8e-25
XP_013639204.1 PREDICTED: uncharacterized protein LOC106344352 [... 77 1e-24
GAV81240.1 zf-RVT domain-containing protein, partial [Cephalotus... 109 1e-24
BAB09379.1 non-LTR retroelement reverse transcriptase-like prote... 87 2e-24
GAV83549.1 zf-RVT domain-containing protein [Cephalotus follicul... 100 2e-24
GAV89202.1 zf-RVT domain-containing protein [Cephalotus follicul... 100 2e-24
XP_013617955.1 PREDICTED: uncharacterized protein LOC106324550 [... 86 3e-24
GAV85439.1 zf-RVT domain-containing protein [Cephalotus follicul... 99 3e-24
GAV67837.1 zf-RVT domain-containing protein, partial [Cephalotus... 98 4e-24
>GAV70450.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 497
Score = 100 bits (249), Expect(2) = 1e-26
Identities = 47/126 (37%), Positives = 72/126 (57%), Gaps = 1/126 (0%)
Frame = +2
Query: 1274 VIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLFF 1450
V W K +W +P+HSFC W+ H+ T+D+LL WG+++S+ C+F C G E++DHLFF
Sbjct: 326 VPWWKMVWCSKRIPKHSFCLWLTIHRAHKTKDKLLNWGLISSARCVFHCGGTESMDHLFF 385
Query: 1451 GCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNE 1630
C ++ I L + R + W EE + + G F + L +L + VY +W E
Sbjct: 386 ECPYTRNIWRDALRRCNIHRNILPWVEEEEWLEAYAKGDYFPAILMRLAVGATVYHIWLE 445
Query: 1631 RNKRAF 1648
RN+R F
Sbjct: 446 RNRRCF 451
Score = 50.1 bits (118), Expect(2) = 1e-26
Identities = 33/125 (26%), Positives = 54/125 (43%), Gaps = 2/125 (1%)
Frame = +3
Query: 807 RRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNGM 986
+RSLW+ + I + + W + + SW W+ LK ++ R H+ G G
Sbjct: 176 QRSLWAKWCNLHLIRKHNLWSLHNWGS------HSWVWRHMLKLRDIARTHMVYECGRGE 229
Query: 987 QTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEIQEVV 1160
+ W+D W +H + Y R+ K EVI R WP +E Q+ V
Sbjct: 230 LFSLWYDPWFHGNCIHALYGHRVIYDERMNGNEKLKEVIVNGRWSWPQTSWELIETQQRV 289
Query: 1161 RDLPV 1175
+++PV
Sbjct: 290 QNIPV 294
>GAV68715.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 380
Score = 101 bits (252), Expect(2) = 2e-26
Identities = 49/127 (38%), Positives = 70/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W +W +P+H+FC W+ + TRD+LL WGV+ S+SC+F C E+++HLF
Sbjct: 222 EVGWHSLVWHPARIPKHAFCLWLTMRRAHRTRDKLLDWGVINSASCVFNCGEAESLEHLF 281
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I L + R W +E+Q + GK F S + KL VY +W
Sbjct: 282 FHCPFSQNIWGAALSMCNILRPISQWSDEVQWMLDHAKGKKFPSLVRKLAFAASVYHIWL 341
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 342 ERNRRCF 348
Score = 48.5 bits (114), Expect(2) = 2e-26
Identities = 32/129 (24%), Positives = 57/129 (44%), Gaps = 2/129 (1%)
Frame = +3
Query: 795 DYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHI 974
D V +SLW+ V I + + W ++ + SW+W+Q L + ++H+
Sbjct: 69 DIVINQSLWARWCQVYLIKQSNFW------SLPTTGLHSWSWRQILLLRPVAKEHLIYKC 122
Query: 975 GNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEI 1148
G G + + W D W +H + + + A EVI + R WP ++I
Sbjct: 123 GRGDKFSLWFDPWMHGESVHTLYGNRVIHDSHLGRLALVREVIREGRWDWPANSMDLVDI 182
Query: 1149 QEVVRDLPV 1175
Q V+D+P+
Sbjct: 183 QTSVQDIPI 191
>GAV61706.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 493
Score = 102 bits (253), Expect(2) = 3e-26
Identities = 49/127 (38%), Positives = 70/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W +W +P+H+FC W+ + TRD+LL WGV+ S+SC+F C E+++HLF
Sbjct: 321 EVGWHSLVWHPARIPKHAFCLWLTMRRAHRTRDKLLDWGVINSASCVFNCGEAESLEHLF 380
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I L + R W +E+Q + GK F S + KL VY +W
Sbjct: 381 FHCPFSQNIWGAALSMCNILRPISQWSDEVQWMLDHAKGKKFPSLVLKLAFAASVYHIWL 440
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 441 ERNRRCF 447
Score = 47.0 bits (110), Expect(2) = 3e-26
Identities = 32/129 (24%), Positives = 57/129 (44%), Gaps = 2/129 (1%)
Frame = +3
Query: 795 DYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHI 974
D V +SLW+ V I + + W ++ + SW+W+Q L + ++H+
Sbjct: 168 DIVINQSLWARWCQVYLIKQSNFW------SLPTTGLHSWSWRQILLLRPVAKEHLIYKC 221
Query: 975 GNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEI 1148
G G + + W D W +H + + + A EVI + R WP ++I
Sbjct: 222 GRGDKFSLWFDPWMHGESVHTLYGNRVIHDSHLGRLALVREVIREGRWDWPENSTDLVDI 281
Query: 1149 QEVVRDLPV 1175
Q V+D+P+
Sbjct: 282 QISVQDIPI 290
>GAV66493.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 493
Score = 101 bits (252), Expect(2) = 2e-25
Identities = 48/127 (37%), Positives = 71/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W +W +P+H+FC W+ + TRD+LL WGV+ S+SC+F C E+++HLF
Sbjct: 321 EVSWHSLVWHPARIPKHAFCLWLTMRRAHRTRDKLLDWGVINSASCVFYCGEAESLEHLF 380
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I +L + R W +E+Q + G+ F S + KL VY +W
Sbjct: 381 FHCPFSQNIWGAVLSMCNILRPISQWSDEVQWMLDHAKGQKFPSLVRKLAFAASVYHIWL 440
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 441 ERNRRCF 447
Score = 44.7 bits (104), Expect(2) = 2e-25
Identities = 31/129 (24%), Positives = 57/129 (44%), Gaps = 2/129 (1%)
Frame = +3
Query: 795 DYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHI 974
+ V +SLW+ V I + + W ++ SW+W+Q L + ++H+
Sbjct: 168 EIVINQSLWARWCHVYLIKQSNFW------SLPTIGLHSWSWRQILLLRPVAKEHLIYKC 221
Query: 975 GNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEI 1148
G G + + W D W +H + + ++ A EVI + R WP ++I
Sbjct: 222 GRGDKFSLWFDPWMHGESVHILYGNRVIHDSQLGRLALVKEVIREGRWDWPANSTDLVDI 281
Query: 1149 QEVVRDLPV 1175
Q V+D+P+
Sbjct: 282 QISVQDIPI 290
>GAV92989.1 zf-RVT domain-containing protein, partial [Cephalotus follicularis]
Length = 397
Score = 97.8 bits (242), Expect(3) = 2e-25
Identities = 46/124 (37%), Positives = 68/124 (54%), Gaps = 1/124 (0%)
Frame = +2
Query: 1280 WSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLFFGC 1456
W + +W +P+H+F W+ TRD+L+ GV S+ C+F C G E++DHLFF C
Sbjct: 226 WHQLVWHPKRIPKHAFSLWLAIRGAHRTRDKLMAMGVTHSAQCVFHCGGTESLDHLFFQC 285
Query: 1457 AFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNERN 1636
FS ++ +L + R + WD+E+Q G+ F + KL L VY VW ERN
Sbjct: 286 PFSASVWRDVLKLCNITRPILSWDDEVQWMTDHARGRKFHHLVRKLALAAAVYHVWRERN 345
Query: 1637 KRAF 1648
+R F
Sbjct: 346 RRCF 349
Score = 46.2 bits (108), Expect(3) = 2e-25
Identities = 28/93 (30%), Positives = 41/93 (44%), Gaps = 2/93 (2%)
Frame = +3
Query: 909 SWAWKQFLKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYK--LRILEE 1082
SW+W+Q L + R+H+ GNG + + W D W + +H + Y L
Sbjct: 102 SWSWRQTLLLRPLAREHIIYQCGNGERFSLWFDPWLQGESIHARYGRRVMYDTGLGCHAR 161
Query: 1083 AKDAEVINQRRWPIGRRLTLEIQEVVRDLPV*T 1181
KD Q WP LEIQ+ V +P+ T
Sbjct: 162 VKDVLCEGQWNWPQVSGDLLEIQQRVNVIPIST 194
Score = 22.3 bits (46), Expect(3) = 2e-25
Identities = 7/16 (43%), Positives = 10/16 (62%)
Frame = +1
Query: 1732 RNSAN*KICMEWGFPM 1779
RN + +C+ WG PM
Sbjct: 379 RNDHHDSLCVNWGVPM 394
>GAV75138.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 230
Score = 114 bits (285), Expect = 3e-25
Identities = 55/127 (43%), Positives = 76/127 (59%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLF 1447
KV W+ +WF C+P+HSFC W+ CH TRD+L ++GVV S+ CIF GN ETIDH F
Sbjct: 49 KVNWANMVWFSNCIPKHSFCLWLSCHYARWTRDKLHRFGVVGSNRCIFGCGNVETIDHSF 108
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
FGC F+GA+ + L R W+ E + + + GK+F+ + K+ L +Y W
Sbjct: 109 FGCRFTGAVWKQTLAKCGFKRIVQPWNGEAKWVILRLRGKNFKIWITKIALATCIYHCWM 168
Query: 1628 ERNKRAF 1648
ERN R F
Sbjct: 169 ERNSRIF 175
>GAV74389.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 493
Score = 99.8 bits (247), Expect(2) = 4e-25
Identities = 48/127 (37%), Positives = 70/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W +W +P+H+FC W+ + TRD+LL WGV+ S+SC+F C E+++HLF
Sbjct: 321 EVGWHSLVWHPARIPKHAFCLWLAMRRAHRTRDKLLAWGVINSASCVFNCGEVESLEHLF 380
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I L + R W +E+Q + G+ F S + KL VY +W
Sbjct: 381 FHCPFSQNIWGAALSMCNILRPISQWSDEVQWMLDHARGQKFPSLVRKLAFAASVYHIWL 440
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 441 ERNRRCF 447
Score = 45.8 bits (107), Expect(2) = 4e-25
Identities = 32/129 (24%), Positives = 56/129 (43%), Gaps = 2/129 (1%)
Frame = +3
Query: 795 DYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHI 974
D V +SLW+ V I + + W ++ SW+W+Q L + ++H+
Sbjct: 168 DIVINQSLWARWCHVYLIKQSNFW------SLPTIGLHSWSWRQILLLRPVAKEHLIYKC 221
Query: 975 GNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEI 1148
G G + + W D W +H + + + A EVI + R WP ++I
Sbjct: 222 GRGDKFSLWFDPWMHGESVHTLYGNRVIHDSLLGRLALVKEVIREGRWDWPANSTDLVDI 281
Query: 1149 QEVVRDLPV 1175
Q V+D+P+
Sbjct: 282 QISVQDIPI 290
>GAV77512.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 394
Score = 100 bits (248), Expect(2) = 4e-25
Identities = 49/127 (38%), Positives = 70/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W +W +P+H+FC W+ + TRD+LL WGV+ S+SC+F C E+++HLF
Sbjct: 222 EVGWHSLVWHPVRIPKHAFCLWLTMRRAHRTRDKLLYWGVINSASCVFNCGEAESLEHLF 281
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I L + R W +E+Q + GK F S + KL VY +W
Sbjct: 282 FHCPFSQNIWGAALSMCNILRPISQWSDEVQWMLDHAKGKKFPSLVRKLAFAASVYHIWL 341
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 342 ERNRRCF 348
Score = 45.4 bits (106), Expect(2) = 4e-25
Identities = 31/129 (24%), Positives = 56/129 (43%), Gaps = 2/129 (1%)
Frame = +3
Query: 795 DYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHI 974
D V +SLW V I + + W ++ + SW+W+Q L + ++H+
Sbjct: 69 DIVINQSLWVRWCQVYLIKQSNFW------SLPTTGLHSWSWRQILLLRPVAKEHLIYKC 122
Query: 975 GNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WPIGRRLTLEI 1148
G G + + W D W +H + + + A +VI + R WP ++I
Sbjct: 123 GRGDKFSLWFDPWMHGESVHTLYGNRVIHDSHLGRLALVRDVIREGRWDWPANSTDLVDI 182
Query: 1149 QEVVRDLPV 1175
Q V+D+P+
Sbjct: 183 QISVQDIPI 191
>XP_018473660.1 PREDICTED: uncharacterized protein LOC108844871 [Raphanus sativus]
Length = 1782
Score = 85.9 bits (211), Expect(2) = 5e-25
Identities = 53/159 (33%), Positives = 77/159 (48%), Gaps = 5/159 (3%)
Frame = +2
Query: 1175 LNEGLEDRLIWDGNGAKVRI---SHF*KVDCPVEEKVIWSK*LWFKGCVPRHSFCCWVLC 1345
LN + D +WD + + + +V P + K W +WFKG VP+H+F WV
Sbjct: 1582 LNANVSDAYVWDAGSSPLSAFSSAATWEVMRPRQGKKEWFDVVWFKGAVPKHAFTMWVAN 1641
Query: 1346 HKKLPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLFFGCAFSGAIRSKLL-MYMDDFREPM 1519
+ +LPTR RL WGV S C C + ET DHLF C +S AI S++L F
Sbjct: 1642 YNRLPTRARLANWGVPVSPLCGLCATHAETRDHLFLSCRYSEAIWSEVLCRCQSTFVLFT 1701
Query: 1520 VWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNERN 1636
W E + S + S L K+ + ++ +W +RN
Sbjct: 1702 EWSELLSWIRSAPSAQ--LSLLRKIAVQATIFHIWKQRN 1738
Score = 59.3 bits (142), Expect(2) = 5e-25
Identities = 30/85 (35%), Positives = 46/85 (54%), Gaps = 1/85 (1%)
Frame = +3
Query: 867 LIGGDF-NVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVL 1043
L+G F ++ + DSWAWK+ LK + + IGNG+ T+FW D+W +G L E +
Sbjct: 1474 LLGHSFWSIAPAQTDSWAWKRLLKLRPLASRFCVSVIGNGVTTSFWFDNWTPLGPLIEFI 1533
Query: 1044 SKDAKYKLRILEEAKDAEVINQRRW 1118
LR+ + A A+ I + RW
Sbjct: 1534 GPSGPRALRLRKGAMVADAIRETRW 1558
>GAV93027.1 zf-RVT domain-containing protein, partial [Cephalotus follicularis]
Length = 507
Score = 94.7 bits (234), Expect(2) = 5e-25
Identities = 50/154 (32%), Positives = 74/154 (48%), Gaps = 1/154 (0%)
Frame = +2
Query: 1190 EDRLIWDGNGAKVRISHF*KVDCPVEEKVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRD 1369
+D + W G S V P KV W +W G +P+H+FC W+ TRD
Sbjct: 306 QDSIYWAKVGDSFSTSRAWNVIRPRSNKVGWHALVWHPGRIPKHAFCLWLAVRGAHRTRD 365
Query: 1370 RLLKWGVVTSSSCIF-CEGNETIDHLFFGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVA 1546
+LL GV ++ C+F C E+ +HLFF C F+ + ++L + R M W E++
Sbjct: 366 KLLAMGVTQTAQCVFHCGEPESTEHLFFLCPFTAYVWIEVLKLCNITRNIMPWPYELRWM 425
Query: 1547 VSKYGGKSFRSRLYKLCLNCVVYEVWNERNKRAF 1648
+ F R+ KL VY +W ERN+R F
Sbjct: 426 SAHARDNQFHHRIRKLAFASTVYHIWRERNRRCF 459
Score = 50.4 bits (119), Expect(2) = 5e-25
Identities = 25/93 (26%), Positives = 48/93 (51%), Gaps = 2/93 (2%)
Frame = +3
Query: 909 SWAWKQFLKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAK 1088
SW+W+Q L + R+H+ GNG + + W D W + +H + + Y + +A+
Sbjct: 212 SWSWRQILLLRPLAREHLIYKCGNGERFSLWFDPWLQGESIHALYGRRVIYDSGLGSQAR 271
Query: 1089 DAEVINQRR--WPIGRRLTLEIQEVVRDLPV*T 1181
+++ + + WP +EIQ+ V D+P+ T
Sbjct: 272 VKDILREGQWCWPQTSGDLMEIQQKVDDIPIST 304
>XP_013588944.1 PREDICTED: uncharacterized protein LOC106297205 [Brassica oleracea
var. oleracea]
Length = 776
Score = 82.0 bits (201), Expect(2) = 8e-25
Identities = 53/158 (33%), Positives = 77/158 (48%), Gaps = 4/158 (2%)
Frame = +2
Query: 1181 EGLEDRLIWDG-NGAKVRIS-HF*KVDCPVEE-KVIWSK*LWFKGCVPRHSFCCWVLCHK 1351
E ++D +W +G K R S H + C E +V W K +WF C P+ SF W+
Sbjct: 574 ENMQDTDLWRRRSGFKKRFSNHETWLLCRQERTEVDWDKGIWFSHCTPKFSFMIWLAMLN 633
Query: 1352 KLPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLFFGCAFSGAIRSKLLMYMDDFREPMVWD 1528
KL T DR+ W T+ +C+ C ET DHLFF C++S I KL+ + R W
Sbjct: 634 KLSTLDRVATWNRGTNPTCVLCSVEVETRDHLFFKCSYSTMIWEKLVKGIMKRRYSDNWS 693
Query: 1529 EEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNERNKR 1642
+ V G + + ++ C +Y VW ERN+R
Sbjct: 694 FITNMIVDS-GMEPISTFCFRYSFQCALYTVWFERNRR 730
Score = 62.4 bits (150), Expect(2) = 8e-25
Identities = 40/130 (30%), Positives = 61/130 (46%)
Frame = +3
Query: 804 DRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNG 983
++ SLW + + + S W I G K+ SW WK+ LK ++ + IGNG
Sbjct: 450 EKTSLWGQWIRMNLTKKKSFWEISG-----KTQQGSWMWKKILKLRDIAKTFHMKDIGNG 504
Query: 984 MQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRRWPIGRRLTLEIQEVVR 1163
T+ W D W MG + E+L L I E+ AE ++R P R T+ +QEV +
Sbjct: 505 RNTSLWFDQWTDMGQMSELLGDRGIIDLGIPRESNVAEAFTRQR-PRRRHRTVILQEVEK 563
Query: 1164 DLPV*TKGWR 1193
+ + T R
Sbjct: 564 QIMIYTNSRR 573
>GAV91388.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 288
Score = 101 bits (251), Expect(2) = 8e-25
Identities = 52/153 (33%), Positives = 79/153 (51%), Gaps = 1/153 (0%)
Frame = +2
Query: 1193 DRLIWDGNGAKVRISHF*KVDCPVEEKVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDR 1372
D + W G I ++ + V W K +WF +P+HSFC W + T+D+
Sbjct: 90 DCIGWHKRGGAFSIKQAWQLIRRASDIVPWWKLVWFSKRIPKHSFCLWFTILRAHKTKDK 149
Query: 1373 LLKWGVVTSSSCI-FCEGNETIDHLFFGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAV 1549
LL WG++ S+SC+ C G E++DHLFF C ++ I L + R + W EE+Q
Sbjct: 150 LLNWGLIMSASCVSHCGGAESMDHLFFECPYARDIWRDTLRICNIHRNILPWVEEVQWLE 209
Query: 1550 SKYGGKSFRSRLYKLCLNCVVYEVWNERNKRAF 1648
+ G F + L +L + VY +W ERN+R F
Sbjct: 210 AYASGDYFSAILMRLAVGATVYHIWLERNRRCF 242
Score = 43.1 bits (100), Expect(2) = 8e-25
Identities = 25/84 (29%), Positives = 40/84 (47%), Gaps = 2/84 (2%)
Frame = +3
Query: 930 LKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRI--LEEAKDAEVI 1103
LK ++ R H+ G G + W+D W +H Y +R+ E+ K+A V
Sbjct: 2 LKLRDLARTHMVYECGRGEHFSLWYDPWFHGNSIHAWYGHRVIYDVRMNGTEKLKEAFVN 61
Query: 1104 NQRRWPIGRRLTLEIQEVVRDLPV 1175
+ WP +EIQ+ V+D+PV
Sbjct: 62 GRWSWPQTSWQLIEIQQRVQDIPV 85
>XP_013639204.1 PREDICTED: uncharacterized protein LOC106344352 [Brassica oleracea
var. oleracea]
Length = 509
Score = 77.4 bits (189), Expect(2) = 1e-24
Identities = 44/123 (35%), Positives = 66/123 (53%), Gaps = 4/123 (3%)
Frame = +3
Query: 813 SLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNGMQT 992
SLW V + I +GS W I G + SW WK+ LKH++ + V + NG++
Sbjct: 235 SLWVRWVQIYLIRKGSLWSIKGTTQM-----GSWMWKKILKHRDRAKLFYMVDVRNGLKA 289
Query: 993 NFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQR----RWPIGRRLTLEIQEVV 1160
+FW++ W MG L E+L K ++ I E AK AE IN R R+ + R+ LEI+++
Sbjct: 290 SFWYEKWSPMGRLIEILGKGRYIEMGIKENAKVAECINHRKKNHRFSMLNRVELEIEKIK 349
Query: 1161 RDL 1169
L
Sbjct: 350 EKL 352
Score = 66.2 bits (160), Expect(2) = 1e-24
Identities = 34/99 (34%), Positives = 55/99 (55%), Gaps = 3/99 (3%)
Frame = +2
Query: 1280 WSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLFFGC 1456
W K +WFK P++SF WV +L T R+ +W + ++SC FC+ E+I+HLFF C
Sbjct: 391 WYKAIWFKHYTPKYSFILWVAMRDRLSTGSRMAQWDISANTSCSFCQDPLESIEHLFFQC 450
Query: 1457 AFSGAIRSKLL--MYMDDFREPMVWDEEMQVAVSKYGGK 1567
+F+ I L + D+F W E ++ + +GG+
Sbjct: 451 SFTSLIWENLARGVLKDNFTAR--WSEIKRICII-FGGR 486
>GAV81240.1 zf-RVT domain-containing protein, partial [Cephalotus follicularis]
Length = 471
Score = 109 bits (272), Expect(2) = 1e-24
Identities = 57/153 (37%), Positives = 78/153 (50%), Gaps = 1/153 (0%)
Frame = +2
Query: 1193 DRLIWDGNGAKVRISHF*KVDCPVEEKVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDR 1372
DR+ W G I V +V W K +WF G +P+H FC W+ + T D+
Sbjct: 251 DRIHWMKKGGTFTIRDACNVINMQGSEVEWWKIVWFPGSIPKHCFCVWLTFWEARRTLDK 310
Query: 1373 LLKWGVVTSSSCIF-CEGNETIDHLFFGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAV 1549
L++WGVV++S+C F C E+IDHLFF C F+ + L R P W EE +
Sbjct: 311 LVRWGVVSTSNCCFSCGQEESIDHLFFSCPFTARVWKHFLGLCGFRRRPRGWREESVWCI 370
Query: 1550 SKYGGKSFRSRLYKLCLNCVVYEVWNERNKRAF 1648
S+ G F+ + KL L V+Y W ERN R F
Sbjct: 371 SRLKGNGFKLWITKLTLAAVLYHCWQERNNRLF 403
Score = 34.3 bits (77), Expect(2) = 1e-24
Identities = 22/104 (21%), Positives = 44/104 (42%)
Frame = +3
Query: 807 RRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNGM 986
+ S+W+ + S W ++N S++ + LK + + ++ IG+GM
Sbjct: 128 KASIWTDWCYKVFLKENSFWAAKLNYNC------SYSRRNVLKARNLLANYLHYEIGDGM 181
Query: 987 QTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRRW 1118
T+ W D W L + + + R+ A+ + VI + W
Sbjct: 182 STSLWFDPWLSGVSLVDRYGESVIQESRLQRNARLSSVIKEGEW 225
>BAB09379.1 non-LTR retroelement reverse transcriptase-like protein [Arabidopsis
thaliana]
Length = 1223
Score = 87.4 bits (215), Expect(2) = 2e-24
Identities = 50/157 (31%), Positives = 75/157 (47%), Gaps = 6/157 (3%)
Frame = +2
Query: 1190 EDRLIWDGNGAKVRISHF*KVDC-----PVEEKVIWSK*LWFKGCVPRHSFCCWVLCHKK 1354
ED+++W G R + F D +V W K +WF P++SFC W+ H +
Sbjct: 1022 EDKVLWRGKSDVFRTT-FSTRDTWHHTRSTSARVPWHKVIWFSHATPKYSFCSWLAAHGR 1080
Query: 1355 LPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLFFGCAFSGAIRSKLLMYMDDFREPMVWDE 1531
LPT DR++ W ++ CIFC+G ET DHLFF C+F+ I L + + W
Sbjct: 1081 LPTGDRMINWANGIATDCIFCQGTLETRDHLFFTCSFTSVIWVDLARGIFKTQYTSHWQS 1140
Query: 1532 EMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNERNKR 1642
++ A++ L + +Y VW ERN R
Sbjct: 1141 IIE-AITNSQHHRVEWFLRRYVFQATIYIVWRERNGR 1176
Score = 55.8 bits (133), Expect(2) = 2e-24
Identities = 33/96 (34%), Positives = 45/96 (46%)
Frame = +3
Query: 909 SWAWKQFLKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAK 1088
SW WK+ LK++E + KV +GNG QT+FW+D+W +G L E L I
Sbjct: 927 SWIWKKLLKYREVAKTLSKVEVGNGKQTSFWYDNWSDLGQLLERTGDRGLIDLGISRRMT 986
Query: 1089 DAEVINQRRWPIGRRLTLEIQEVVRDLPV*TKGWRT 1196
E RR RR ++ V+ D K W T
Sbjct: 987 VEEAWTNRR---QRRHRNDVYNVIED--ALKKSWDT 1017
Score = 43.1 bits (100), Expect(2) = 4e-07
Identities = 24/63 (38%), Positives = 37/63 (58%), Gaps = 2/63 (3%)
Frame = +3
Query: 705 FVSEQHIFCEVEPLDSKIDKFFLSAIYGANDYVDRRSLWSSLVGVEN--IVRGSPWLIGG 878
+ S Q + C V+ L+ + D+FF S +Y +N +R+ LWS L + I+R PW + G
Sbjct: 83 YKSCQLLTCSVK-LEDRQDEFFCSFVYASNYVEERKVLWSELKDHYDSPIIRHKPWTLLG 141
Query: 879 DFN 887
DFN
Sbjct: 142 DFN 144
Score = 41.2 bits (95), Expect(2) = 4e-07
Identities = 22/70 (31%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Frame = +2
Query: 479 WNVRGINCPIK*YRIKKVCCK--YDIICVIENIVRLNSFDSIFSSIFPGWGCVHNTSAEK 652
WNVRG+N K IKK + + C++E V+ + + +F W + N +
Sbjct: 6 WNVRGLNKSSKHSVIKKWIEENNFQFGCLVETRVKESKVSQLVGKLFKDWSILTNYEHNR 65
Query: 653 VRRIWVCWNK 682
RIWV W K
Sbjct: 66 RGRIWVLWRK 75
>GAV83549.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 242
Score = 99.8 bits (247), Expect(2) = 2e-24
Identities = 46/126 (36%), Positives = 74/126 (58%), Gaps = 1/126 (0%)
Frame = +2
Query: 1274 VIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLFF 1450
V W K +WF +P+HSFC W+ + T+++LL WG+++S+ C+F C G+ +IDHLF
Sbjct: 117 VPWCKLVWFSKRIPKHSFCLWLTILRAHKTKNKLLNWGLISSARCVFHCGGSGSIDHLFS 176
Query: 1451 GCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWNE 1630
C ++ I L + F+ + W E++Q + G +F + L +L L VY +W E
Sbjct: 177 ECPYTRDIWKDALRRCNIFKNILPWVEQVQWLKAYARGNNFPTVLMRLALGATVYHIWLE 236
Query: 1631 RNKRAF 1648
RN+R F
Sbjct: 237 RNRRCF 242
Score = 43.5 bits (101), Expect(2) = 2e-24
Identities = 26/84 (30%), Positives = 39/84 (46%), Gaps = 2/84 (2%)
Frame = +3
Query: 930 LKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQ 1109
L ++ R H+ G G Q + W+D W +H + Y R+ K EVI
Sbjct: 2 LNLRDLARTHLVYECGRGEQFSLWYDPWFHGNSIHAWCGQRVIYNARMNGTEKLKEVIIN 61
Query: 1110 RR--WPIGRRLTLEIQEVVRDLPV 1175
R WP +EIQ++V+D+PV
Sbjct: 62 GRWSWPRTSWQLIEIQQMVQDIPV 85
>GAV89202.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 395
Score = 100 bits (249), Expect(2) = 2e-24
Identities = 48/127 (37%), Positives = 71/127 (55%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W + +W +P+H FC W+ + TRD+LL WGV+ S+SC+F C E++DHLF
Sbjct: 222 EVGWHRLVWHPAMIPKHVFCLWLAMRRAHRTRDKLLAWGVINSASCVFNCGEVESLDHLF 281
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I +L + R W +E+Q + + G F + + KL VY +W
Sbjct: 282 FLCPFSQNIWGAVLSMCNILRPISHWSDEVQRMLDRARGHKFPTLVRKLAFAASVYHIWL 341
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 342 ERNRRCF 348
Score = 42.4 bits (98), Expect(2) = 2e-24
Identities = 34/138 (24%), Positives = 59/138 (42%), Gaps = 2/138 (1%)
Frame = +3
Query: 768 FLSAIYGANDYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEG 947
FL I+ D V +SLW+ V I + + W ++ SW+W+Q L +
Sbjct: 63 FLKQIW---DIVINQSLWARWCHVYLIKQSNFW------SLPTIGLHSWSWRQILLLRPV 113
Query: 948 VRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRR--WP 1121
++H+ G + + W D W +H + + + A EVI + R WP
Sbjct: 114 AKEHLIYKCGRVDKFSLWFDPWMHGESVHTLYGHRVIHDSLLGRLALVKEVIREGRWDWP 173
Query: 1122 IGRRLTLEIQEVVRDLPV 1175
++IQ V+D+P+
Sbjct: 174 ANSTDLVDIQLSVQDIPI 191
>XP_013617955.1 PREDICTED: uncharacterized protein LOC106324550 [Brassica oleracea
var. oleracea]
Length = 1593
Score = 86.3 bits (212), Expect(2) = 3e-24
Identities = 43/125 (34%), Positives = 69/125 (55%), Gaps = 1/125 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIFC-EGNETIDHLF 1447
+V+WS+ +WF +PRH+F W+ +L T DR+ KWG++ C+ C E NET DHLF
Sbjct: 1425 EVVWSRSVWFTQGIPRHAFLVWLAIQNRLSTGDRMRKWGIL--QGCVLCGERNETRDHLF 1482
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C +S + +L + R W + ++++ + + + L +L VVY +W
Sbjct: 1483 FVCPYSYTVWDRLASRLVGRRINPDWHDMLRLSCTIFSNE-MDMILIRLVFQAVVYYIWR 1541
Query: 1628 ERNKR 1642
ERN R
Sbjct: 1542 ERNTR 1546
Score = 56.2 bits (134), Expect(2) = 3e-24
Identities = 35/140 (25%), Positives = 62/140 (44%), Gaps = 10/140 (7%)
Frame = +3
Query: 810 RSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEGVRKHVKVHIGNGMQ 989
+SLW S + + S W + D SW W++ LK ++ + ++V +GNG
Sbjct: 1269 KSLWVSWIQHYLLRHSSFWDVRDD------TLGSWIWRKLLKMRDMAYQFIRVEVGNGNC 1322
Query: 990 TNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRRWPIGRRLTLEIQEV---- 1157
FWH W +MG L + L + AK ++ +++ +W I + + E+
Sbjct: 1323 AFFWHGDWLRMGKLINITGATGTRYLGVPRNAKVSDAVSEGQWKIRGQRSRHYHELHHRV 1382
Query: 1158 -VRDLPV*TKG-----WRTD 1199
+ +P +KG WR D
Sbjct: 1383 QIEPVPNVSKGPDTFLWRYD 1402
Score = 45.8 bits (107), Expect(2) = 3e-07
Identities = 29/82 (35%), Positives = 43/82 (52%), Gaps = 9/82 (10%)
Frame = +3
Query: 681 KLVFMWEGFV-------SEQHIFCEVEPLDSKIDKFFLSAIYGANDYVDRRSLWSSLVGV 839
K+ F W V S Q I C V+ ++K ++F SA+Y +N V+RR LW + G
Sbjct: 658 KIWFCWSDRVQVTMLHKSSQVITCAVQIPETK-EQFICSAVYASNFEVERRELWEDMRGT 716
Query: 840 ENIVR--GSPWLIGGDFNVVKS 899
+ PW++ GDFNV+ S
Sbjct: 717 LAAYKHLDMPWILLGDFNVMLS 738
Score = 38.9 bits (89), Expect(2) = 3e-07
Identities = 18/69 (26%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Frame = +2
Query: 479 WNVRGINCPIK*YRIKKVCCKYDII--CVIENIVRLNSFDSIFSSIFPGWGCVHNTSAEK 652
WN+R N K + + K + C++E VR + + PGW + N +
Sbjct: 596 WNMRVFNMSRKHVAVHRWIMKEKPLFGCLLETRVREENHSKCMKAALPGWSSIINYDHHR 655
Query: 653 VRRIWVCWN 679
+ +IW CW+
Sbjct: 656 LGKIWFCWS 664
>GAV85439.1 zf-RVT domain-containing protein [Cephalotus follicularis]
Length = 315
Score = 99.4 bits (246), Expect(2) = 3e-24
Identities = 47/127 (37%), Positives = 72/127 (56%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIFCEGN-ETIDHLF 1447
+V W + +W +P+H+FC W+ + TRD+LL WGV++S+SC+F G E+++HLF
Sbjct: 140 EVGWHRLVWHPARIPKHAFCLWLAMRRAHRTRDKLLAWGVISSASCVFNYGEVESLEHLF 199
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I +L + R W +E+Q + G F + + KL VY +W
Sbjct: 200 FHCPFSHNIWGAVLSMCNILRPISQWSDEVQWMLDHARGHKFPTLVRKLAFAASVYHIWL 259
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 260 ERNRRCF 266
Score = 43.1 bits (100), Expect(2) = 3e-24
Identities = 24/91 (26%), Positives = 42/91 (46%), Gaps = 2/91 (2%)
Frame = +3
Query: 909 SWAWKQFLKHKEGVRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAK 1088
SW+W+Q L + ++H+ G G + + W D W +H + + + A
Sbjct: 19 SWSWRQILLLRPVAKEHLIYKCGRGDKFSLWFDPWMHGESVHTLYGHRVIHDPLLGRLAL 78
Query: 1089 DAEVINQRR--WPIGRRLTLEIQEVVRDLPV 1175
EVI + R WP ++IQ V+D+P+
Sbjct: 79 VKEVIKEGRWDWPANSTDLVDIQLTVQDIPI 109
>GAV67837.1 zf-RVT domain-containing protein, partial [Cephalotus follicularis]
Length = 454
Score = 98.2 bits (243), Expect(2) = 4e-24
Identities = 48/127 (37%), Positives = 69/127 (54%), Gaps = 1/127 (0%)
Frame = +2
Query: 1271 KVIWSK*LWFKGCVPRHSFCCWVLCHKKLPTRDRLLKWGVVTSSSCIF-CEGNETIDHLF 1447
+V W + +W +P+H+FC W+ + TRD LL W V+ S SC+F C E++DHLF
Sbjct: 281 EVGWHRLVWHPAMIPKHAFCLWLAMRRAHRTRDNLLAWRVINSVSCVFNCGEVESLDHLF 340
Query: 1448 FGCAFSGAIRSKLLMYMDDFREPMVWDEEMQVAVSKYGGKSFRSRLYKLCLNCVVYEVWN 1627
F C FS I +L + R W +E+Q + G F +R+ KL VY +W
Sbjct: 341 FLCPFSQNIWGAVLSMCNILRPISHWSDEVQWMLDHARGHKFPTRVRKLAFAASVYHIWL 400
Query: 1628 ERNKRAF 1648
ERN+R F
Sbjct: 401 ERNRRCF 407
Score = 43.9 bits (102), Expect(2) = 4e-24
Identities = 35/138 (25%), Positives = 60/138 (43%), Gaps = 2/138 (1%)
Frame = +3
Query: 768 FLSAIYGANDYVDRRSLWSSLVGVENIVRGSPWLIGGDFNVVKSCFDSWAWKQFLKHKEG 947
FL I+ D V +SLW+ V I + + W ++ SW+W+Q L +
Sbjct: 122 FLKQIW---DIVINQSLWARWCHVYLIKQSNFW------SLPTIGLHSWSWRQILLLRPV 172
Query: 948 VRKHVKVHIGNGMQTNFWHDSWHKMGVLHEVLSKDAKYKLRILEEAKDAEVINQRRWPIG 1127
++H+ G G + + W D W +H + + + A EVI + RW
Sbjct: 173 AKEHLIYKCGRGDKFSLWFDPWMHGESVHTLYGHRVIHDSLLGRLALVKEVIREGRWDWL 232
Query: 1128 RRLT--LEIQEVVRDLPV 1175
T ++IQ V+D+P+
Sbjct: 233 ANSTDLVDIQLSVQDIPI 250