BLASTX nr result
ID: Lithospermum23_contig00013264
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00013264
(315 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KVI07446.1 1-aminocyclopropane-1-carboxylate deaminase/D-cystein... 203 2e-63
KQL31727.1 hypothetical protein SETIT_017484mg [Setaria italica] 199 5e-62
OEL34276.1 putative D-cysteine desulfhydrase 1, mitochondrial [D... 197 1e-61
OAY70152.1 putative D-cysteine desulfhydrase 1, mitochondrial [A... 196 2e-61
XP_016455254.1 PREDICTED: putative D-cysteine desulfhydrase 1, m... 196 3e-61
XP_017247894.1 PREDICTED: bifunctional D-cysteine desulfhydrase/... 201 3e-61
XP_016563957.1 PREDICTED: putative D-cysteine desulfhydrase 1, m... 201 4e-61
BAD16875.1 putative 1-aminocyclopropane-1-carboxylate deaminase ... 199 4e-61
EEC74091.1 hypothetical protein OsI_09120 [Oryza sativa Indica G... 199 4e-61
ONI09474.1 hypothetical protein PRUPE_5G240500 [Prunus persica] 197 5e-61
XP_002454633.1 hypothetical protein SORBIDRAFT_04g034640 [Sorghu... 199 5e-61
KQL31726.1 hypothetical protein SETIT_017484mg [Setaria italica] 199 6e-61
EOY21568.1 D-cysteine desulfhydrase isoform 2 [Theobroma cacao] 196 7e-61
XP_006647985.2 PREDICTED: putative D-cysteine desulfhydrase 1, m... 199 1e-60
KNA15440.1 hypothetical protein SOVF_098230 [Spinacia oleracea] 198 1e-60
XP_010091758.1 Putative 1-aminocyclopropane-1-carboxylate deamin... 198 1e-60
XP_019175497.1 PREDICTED: putative D-cysteine desulfhydrase 1, m... 199 1e-60
KXG31193.1 hypothetical protein SORBI_004G314500 [Sorghum bicolor] 199 1e-60
XP_015626189.1 PREDICTED: putative D-cysteine desulfhydrase 1, m... 199 1e-60
ONI09473.1 hypothetical protein PRUPE_5G240500 [Prunus persica] 197 1e-60
>KVI07446.1 1-aminocyclopropane-1-carboxylate deaminase/D-cysteine
desulfhydrase [Cynara cardunculus var. scolymus]
Length = 309
Score = 203 bits (517), Expect = 2e-63
Identities = 98/104 (94%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFLLADAV+QGADCI+TIGGIQSNHCRA
Sbjct: 90 WNLPNLPKDTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVAQGADCIITIGGIQSNHCRA 149
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDCFLILRTSKAL+D DPGLTGNLLVERLVGAHID
Sbjct: 150 TAVAAKYLNLDCFLILRTSKALVDKDPGLTGNLLVERLVGAHID 193
>KQL31727.1 hypothetical protein SETIT_017484mg [Setaria italica]
Length = 297
Score = 199 bits (506), Expect = 5e-62
Identities = 93/104 (89%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFL+ADAV+QGADCI+T+GGIQSNHCRA
Sbjct: 53 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITVGGIQSNHCRA 112
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 113 TAVAAKYLNLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 156
>OEL34276.1 putative D-cysteine desulfhydrase 1, mitochondrial [Dichanthelium
oligosanthes]
Length = 269
Score = 197 bits (501), Expect = 1e-61
Identities = 91/104 (87%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFL+ADA++QGADC++T+GGIQSNHCRA
Sbjct: 53 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLMADALAQGADCVITVGGIQSNHCRA 112
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 113 TAVAAKYLNLDCYLILRTSKLLVDKDPGLIGNLLVERLVGAHID 156
>OAY70152.1 putative D-cysteine desulfhydrase 1, mitochondrial [Ananas comosus]
Length = 260
Score = 196 bits (499), Expect = 2e-61
Identities = 90/104 (86%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFL+ADAV+QGADC++T+GGIQSNHCRA
Sbjct: 44 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRA 103
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAA+YLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHI+
Sbjct: 104 TAVAARYLNLDCYLILRTSKVLVDKDPGLVGNLLVERLVGAHIE 147
>XP_016455254.1 PREDICTED: putative D-cysteine desulfhydrase 1, mitochondrial,
partial [Nicotiana tabacum]
Length = 265
Score = 196 bits (498), Expect = 3e-61
Identities = 94/104 (90%), Positives = 98/104 (94%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDD+SGMQLSGNKVRKLEFLL DAV+QGADCIVTIGGIQSNHCRA
Sbjct: 110 WNLPNLPKDTEVWLKRDDISGMQLSGNKVRKLEFLLGDAVAQGADCIVTIGGIQSNHCRA 169
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 170 TAVAAKYLNLDCYLILRTSKLLVDKDPGLIGNLLVERLVGAHID 213
>XP_017247894.1 PREDICTED: bifunctional D-cysteine
desulfhydrase/1-aminocyclopropane-1-carboxylate
deaminase, mitochondrial [Daucus carota subsp. sativus]
Length = 421
Score = 201 bits (510), Expect = 3e-61
Identities = 96/104 (92%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFLLADAV+QGADCIVTIGGIQSNHCRA
Sbjct: 86 WNLPNLPKNTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVAQGADCIVTIGGIQSNHCRA 145
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAH+D
Sbjct: 146 TAVAAKYLNLDCYLILRTSKVLLDKDPGLTGNLLVERLVGAHVD 189
>XP_016563957.1 PREDICTED: putative D-cysteine desulfhydrase 1, mitochondrial
[Capsicum annuum]
Length = 425
Score = 201 bits (510), Expect = 4e-61
Identities = 97/104 (93%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFLLADAV+QGADCIVTIGGIQSNHCRA
Sbjct: 90 WNLPNLPKDTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVAQGADCIVTIGGIQSNHCRA 149
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAHID
Sbjct: 150 TAVAAKYLNLDCYLILRTSKLLVDKDPGLTGNLLVERLVGAHID 193
>BAD16875.1 putative 1-aminocyclopropane-1-carboxylate deaminase [Oryza sativa
Japonica Group] BAF10181.1 Os02g0773300 [Oryza sativa
Japonica Group] EEE57887.1 hypothetical protein
OsJ_08555 [Oryza sativa Japonica Group] BAS81139.1
Os02g0773300 [Oryza sativa Japonica Group]
Length = 385
Score = 199 bits (507), Expect = 4e-61
Identities = 91/104 (87%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLPNGTEVW+KRDD+SGMQLSGNKVRKLEFL+ADAV+QGADC++T+GGIQSNHCRA
Sbjct: 51 WNLPNLPNGTEVWIKRDDISGMQLSGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRA 110
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKY+NLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 111 TAVAAKYINLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 154
>EEC74091.1 hypothetical protein OsI_09120 [Oryza sativa Indica Group]
Length = 385
Score = 199 bits (507), Expect = 4e-61
Identities = 91/104 (87%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLPNGTEVW+KRDD+SGMQLSGNKVRKLEFL+ADAV+QGADC++T+GGIQSNHCRA
Sbjct: 51 WNLPNLPNGTEVWIKRDDISGMQLSGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRA 110
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKY+NLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 111 TAVAAKYINLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 154
>ONI09474.1 hypothetical protein PRUPE_5G240500 [Prunus persica]
Length = 302
Score = 197 bits (500), Expect = 5e-61
Identities = 92/104 (88%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFL+ADAV++GADCI+TIGGIQSNHCRA
Sbjct: 97 WNLPNLPTNTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAKGADCIITIGGIQSNHCRA 156
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAH++
Sbjct: 157 TAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHVE 200
>XP_002454633.1 hypothetical protein SORBIDRAFT_04g034640 [Sorghum bicolor]
Length = 395
Score = 199 bits (507), Expect = 5e-61
Identities = 93/104 (89%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFLLADAV+QGADC++T+GGIQSNHCRA
Sbjct: 61 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLLADAVAQGADCVITVGGIQSNHCRA 120
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 121 TAVAAKYLNLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 164
>KQL31726.1 hypothetical protein SETIT_017484mg [Setaria italica]
Length = 387
Score = 199 bits (506), Expect = 6e-61
Identities = 93/104 (89%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFL+ADAV+QGADCI+T+GGIQSNHCRA
Sbjct: 53 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITVGGIQSNHCRA 112
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 113 TAVAAKYLNLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 156
>EOY21568.1 D-cysteine desulfhydrase isoform 2 [Theobroma cacao]
Length = 305
Score = 196 bits (499), Expect = 7e-61
Identities = 93/103 (90%), Positives = 99/103 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFL+A+AV+QGADCI+TIGGIQSNHCRA
Sbjct: 90 WNLPNLPQNTEVWLKRDDLSGMQLSGNKVRKLEFLMAEAVAQGADCIITIGGIQSNHCRA 149
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHI 311
TAVAAKYLNLDC+LILRTSKAL+D DPGLTGNLLVER VGAHI
Sbjct: 150 TAVAAKYLNLDCYLILRTSKALVDQDPGLTGNLLVERFVGAHI 192
>XP_006647985.2 PREDICTED: putative D-cysteine desulfhydrase 1, mitochondrial,
partial [Oryza brachyantha]
Length = 407
Score = 199 bits (506), Expect = 1e-60
Identities = 92/104 (88%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP+GTEVW+KRDDLSGMQLSGNKVRKLEFL+ADAV+QGADC++T+GGIQSNHCRA
Sbjct: 73 WNLPNLPSGTEVWIKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRA 132
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 133 TAVAAKYLNLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 176
>KNA15440.1 hypothetical protein SOVF_098230 [Spinacia oleracea]
Length = 381
Score = 198 bits (504), Expect = 1e-60
Identities = 95/104 (91%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLPN TEVWLKRDDLSGMQLSGNKVRKLEFL+ADAV+QGAD I+TIGGIQSNHCRA
Sbjct: 46 WNLPNLPNKTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADSIITIGGIQSNHCRA 105
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDCFLILRTSKAL+D DPGLTGNLLVERLVGAH++
Sbjct: 106 TAVAAKYLNLDCFLILRTSKALLDKDPGLTGNLLVERLVGAHVE 149
>XP_010091758.1 Putative 1-aminocyclopropane-1-carboxylate deaminase [Morus
notabilis] EXB45121.1 Putative
1-aminocyclopropane-1-carboxylate deaminase [Morus
notabilis]
Length = 381
Score = 198 bits (504), Expect = 1e-60
Identities = 92/104 (88%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLPN TEVWLKRDD+SGMQLSGNKVRKLEFL+ADAV+QGADCI+TIGGIQSNHCRA
Sbjct: 48 WNLPNLPNNTEVWLKRDDVSGMQLSGNKVRKLEFLMADAVAQGADCIITIGGIQSNHCRA 107
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAA+YLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAH++
Sbjct: 108 TAVAARYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHVE 151
>XP_019175497.1 PREDICTED: putative D-cysteine desulfhydrase 1, mitochondrial
[Ipomoea nil]
Length = 424
Score = 199 bits (507), Expect = 1e-60
Identities = 94/104 (90%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFL+ADAV+QGADCI+T+GGIQSNHCRA
Sbjct: 89 WNLPNLPTNTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAQGADCIITLGGIQSNHCRA 148
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAHID
Sbjct: 149 TAVAAKYLNLDCYLILRTSKVLVDKDPGLTGNLLVERLVGAHID 192
>KXG31193.1 hypothetical protein SORBI_004G314500 [Sorghum bicolor]
Length = 426
Score = 199 bits (507), Expect = 1e-60
Identities = 93/104 (89%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP GTEVW+KRDDLSGMQLSGNKVRKLEFLLADAV+QGADC++T+GGIQSNHCRA
Sbjct: 92 WNLPNLPEGTEVWIKRDDLSGMQLSGNKVRKLEFLLADAVAQGADCVITVGGIQSNHCRA 151
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 152 TAVAAKYLNLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 195
>XP_015626189.1 PREDICTED: putative D-cysteine desulfhydrase 1, mitochondrial
[Oryza sativa Japonica Group] Q6ZHE5.2 RecName:
Full=Putative D-cysteine desulfhydrase 1, mitochondrial;
AltName: Full=OsD-CDes1; Short=D-CDes1; Flags: Precursor
Length = 426
Score = 199 bits (507), Expect = 1e-60
Identities = 91/104 (87%), Positives = 101/104 (97%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLPNGTEVW+KRDD+SGMQLSGNKVRKLEFL+ADAV+QGADC++T+GGIQSNHCRA
Sbjct: 92 WNLPNLPNGTEVWIKRDDISGMQLSGNKVRKLEFLMADAVAQGADCVITVGGIQSNHCRA 151
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKY+NLDC+LILRTSK L+D DPGL GNLLVERLVGAHID
Sbjct: 152 TAVAAKYINLDCYLILRTSKLLVDKDPGLVGNLLVERLVGAHID 195
>ONI09473.1 hypothetical protein PRUPE_5G240500 [Prunus persica]
Length = 339
Score = 197 bits (500), Expect = 1e-60
Identities = 92/104 (88%), Positives = 100/104 (96%)
Frame = +3
Query: 3 WNLPNLPNGTEVWLKRDDLSGMQLSGNKVRKLEFLLADAVSQGADCIVTIGGIQSNHCRA 182
WNLPNLP TEVWLKRDDLSGMQLSGNKVRKLEFL+ADAV++GADCI+TIGGIQSNHCRA
Sbjct: 97 WNLPNLPTNTEVWLKRDDLSGMQLSGNKVRKLEFLMADAVAKGADCIITIGGIQSNHCRA 156
Query: 183 TAVAAKYLNLDCFLILRTSKALIDNDPGLTGNLLVERLVGAHID 314
TAVAAKYLNLDC+LILRTSK L+D DPGLTGNLLVERLVGAH++
Sbjct: 157 TAVAAKYLNLDCYLILRTSKVLVDQDPGLTGNLLVERLVGAHVE 200