BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00011408
         (1122 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001311814.1 myb-related protein Myb4-like [Capsicum annuum] A...   266   4e-84
NP_001312740.1 myb-related protein Myb4-like [Nicotiana tabacum]...   265   1e-83
XP_016743564.1 PREDICTED: myb-related protein Myb4-like [Gossypi...   264   1e-83
XP_019261805.1 PREDICTED: myb-related protein Myb4-like [Nicotia...   263   5e-83
XP_016745702.1 PREDICTED: myb-related protein Myb4-like [Gossypi...   262   8e-83
ABU53925.1 myb-related transcription factor [Datura metel]            262   9e-83
XP_012483245.1 PREDICTED: myb-related protein Myb4-like [Gossypi...   262   1e-82
XP_017640194.1 PREDICTED: myb-related protein Myb4-like [Gossypi...   262   1e-82
XP_009629052.1 PREDICTED: myb-related protein Myb4-like [Nicotia...   261   4e-82
XP_007050304.2 PREDICTED: myb-related protein Myb4 [Theobroma ca...   258   3e-81
XP_004247733.1 PREDICTED: myb-related protein Myb4-like [Solanum...   258   4e-81
XP_015086701.1 PREDICTED: myb-related protein Myb4-like [Solanum...   258   4e-81
EOX94461.1 Myb domain protein 13, putative [Theobroma cacao]          257   6e-81
OMP03097.1 hypothetical protein CCACVL1_02568 [Corchorus capsula...   257   2e-80
XP_002302045.1 GmMYB29 family protein [Populus trichocarpa] EEE8...   255   4e-80
CAA78387.1 protein 2 [Petunia x hybrida]                              255   5e-80
XP_006354484.1 PREDICTED: myb-related protein Myb4-like [Solanum...   254   7e-80
CDY35519.1 BnaA01g23930D [Brassica napus]                             253   2e-79
NP_001268132.1 R2R3 Myb14 transcription factor [Vitis vinifera] ...   252   6e-79
AKV71950.1 R2R3-MYB protein [Rehmannia glutinosa]                     251   6e-79

>NP_001311814.1 myb-related protein Myb4-like [Capsicum annuum] ACB30359.1
           myb-related transcription factor [Capsicum annuum]
          Length = 278

 Score =  266 bits (679), Expect = 4e-84
 Identities = 152/284 (53%), Positives = 186/284 (65%), Gaps = 20/284 (7%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+L+++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILVSYIQANGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTD+EIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDDEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP----PQEAKSEN 398
              PE          KNI++K  ++   Q TL+   N  N K+  NGP    PQ + SE 
Sbjct: 119 YQAPE---NSKRHCKKNIDSKAPSTS--QITLKSSHNFSNIKEGINGPDPNSPQLSSSEV 173

Query: 397 SAMTTMTTSGCSASTT------ESMEKFV--IDDEFWSD--VFSGDCPNLDVNHQFPES- 251
           S +T  + S  +   T      +S E F+  ID+ FW+D  + + D  N D+     E  
Sbjct: 174 STVTADSESVMTMGVTNGGDQMDSYENFIPEIDESFWTDDLLSTADNSNFDMAISGGEEL 233

Query: 250 QFALPSY---MDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
           Q   P Y    + VE D  +  + +MDFWYNVF K GDL EL E
Sbjct: 234 QGQFPFYDMKQESVELDVGAKLEDDMDFWYNVFIKSGDLLELPE 277


>NP_001312740.1 myb-related protein Myb4-like [Nicotiana tabacum] XP_009762172.1
           PREDICTED: myb-related protein Myb4-like [Nicotiana
           sylvestris] BAA88224.1 myb-related transcription factor
           LBM4 [Nicotiana tabacum]
          Length = 281

 Score =  265 bits (676), Expect = 1e-83
 Identities = 148/290 (51%), Positives = 184/290 (63%), Gaps = 26/290 (8%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+L+++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQVLVSYIQTNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP------PQEAKS 404
              P+           N+++K  ++     T    DN  N +++ NGP      PQ + S
Sbjct: 119 YQPPQ---NSKKHSKNNLDSKAPST---SQTFNNSDNFSNIQEDINGPVTGPNSPQRSSS 172

Query: 403 E-------NSAMTTMTTSGCS----ASTTESMEKFV--IDDEFW-SDVFSGDCPNLDVNH 266
           E       ++AMTT+T    +         S E F+  ID+ FW +D+ +GD  N D+  
Sbjct: 173 EMSTVTADSTAMTTITIDDQNMFKQLDEMNSSESFIPEIDESFWTNDLSTGDNSNFDMEG 232

Query: 265 QFPESQFALP------SYMDVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
              E Q   P        MD+V      + +MDFWYNVF K GDL +L E
Sbjct: 233 TGEELQVQFPFSSVKQESMDMV--GTKLEDDMDFWYNVFIKSGDLLDLPE 280


>XP_016743564.1 PREDICTED: myb-related protein Myb4-like [Gossypium hirsutum]
          Length = 275

 Score =  264 bits (675), Expect = 1e-83
 Identities = 139/279 (49%), Positives = 172/279 (61%), Gaps = 15/279 (5%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LIN+I   GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILINYINLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFTREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKPNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
            ++             N+++ KD  +  Q  +       N  D     P E+ SE S +T
Sbjct: 121 GSIDN-----KGQPKSNVDSSKDIKKEQQEPMNFLSPAVNAADNRPASPPESSSEVSTVT 175

Query: 385 TMTTSGCSASTTESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFPESQFAL-PSYM---- 227
           T   +          +++V  ID+ FWS+V S D  ++  + Q   S   L P Y+    
Sbjct: 176 TSENNSNMCMNKIETQEYVSEIDENFWSEVLSADNSSMASDFQVAGSDQQLHPQYLPSSP 235

Query: 226 --------DVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
                   D       +D  MDFWYN+F + GDL EL E
Sbjct: 236 LPKLETTNDYGSNLYDTDTNMDFWYNLFARVGDLSELPE 274


>XP_019261805.1 PREDICTED: myb-related protein Myb4-like [Nicotiana attenuata]
           OIT38235.1 myb-related protein myb4 [Nicotiana
           attenuata]
          Length = 281

 Score =  263 bits (672), Expect = 5e-83
 Identities = 148/290 (51%), Positives = 183/290 (63%), Gaps = 26/290 (8%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+L+++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILVSYIQTNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP------PQEAKS 404
              P+           N+++K  ++     T     N  N +++ NGP      PQ + S
Sbjct: 119 YQPPQ---NSKKHSKNNLDSKAPST---SRTFNNSGNFSNIQEDINGPVTGPNSPQRSSS 172

Query: 403 E-------NSAMTTMTTSGCS----ASTTESMEKFV--IDDEFWSDVFS-GDCPNLDVNH 266
           E       ++AMTT+T    +        +S E F+  ID+ FW+D  S GD  N D+  
Sbjct: 173 EMSTVTADSTAMTTITIDNQNMFKQLDEMDSCESFIPEIDESFWTDDLSTGDNSNFDMEG 232

Query: 265 QFPESQFALP------SYMDVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
              E Q   P        MD+V      + +MDFWYNVF K GDL +L E
Sbjct: 233 TGEELQVQFPFSSVKQESMDMV--GTKLEDDMDFWYNVFIKSGDLLDLPE 280


>XP_016745702.1 PREDICTED: myb-related protein Myb4-like [Gossypium hirsutum]
          Length = 275

 Score =  262 bits (670), Expect = 8e-83
 Identities = 139/279 (49%), Positives = 176/279 (63%), Gaps = 15/279 (5%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LIN+I   GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILINYINLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNF++EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFSREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKPNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
            ++             N+++ KD  +  Q  +       N  D     P E+ SE S +T
Sbjct: 121 GSIEN-----KRQPKSNVDSSKDIKKEQQEPMNFLSPAVNAADNRPASPPESSSEVSTVT 175

Query: 385 TMTTSGCSASTTESMEKFV--IDDEFWSDVFSGD----CPNLDV--NHQFPESQF----A 242
           T   +          +++V  ID+ FWS+V S D      +L V  + Q   SQ+     
Sbjct: 176 TSENNSNMCMNKIETQEYVSEIDENFWSEVLSADNSSIASDLQVAGSDQQLHSQYLPSSP 235

Query: 241 LPSYMDVVEFDPS---SDPEMDFWYNVFTKEGDLQELLE 134
           LP    + ++  +   +D  MDFWYN+F + GDL EL E
Sbjct: 236 LPKLETINDYGSNLYDTDTNMDFWYNLFARVGDLSELPE 274


>ABU53925.1 myb-related transcription factor [Datura metel]
          Length = 278

 Score =  262 bits (670), Expect = 9e-83
 Identities = 146/284 (51%), Positives = 183/284 (64%), Gaps = 20/284 (7%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LI++I+ NGHGNWRALPK AGLL+CGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILISYIQSNGHGNWRALPKLAGLLKCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP---PQEAKSENS 395
              P+           N ++K  ++   Q  L+   N  N +++ NGP   P   +  +S
Sbjct: 119 YQPPQ---TSKRHCKNNNDSKAPSTS--QIALKSSHNFSNIQEDINGPGPGPNSPQRSSS 173

Query: 394 AMTTMTTSGCSAST--------TESMEKFV--IDDEFWSDVFS-GDCPNLD-VNHQFPES 251
            M+T+T    + +          +S E F+  ID+ FW+D  S  D  N + V     E 
Sbjct: 174 EMSTVTADSLAVTMGITNGGGHMDSSENFIPEIDESFWTDDLSTADNSNFNMVGSGGEEL 233

Query: 250 QFALPSY---MDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
           Q   P +    + VE D  +  + +MDFWYNVF K GDL EL E
Sbjct: 234 QVQFPFHAMKQESVEMDVGAKLEDDMDFWYNVFIKSGDLLELPE 277


>XP_012483245.1 PREDICTED: myb-related protein Myb4-like [Gossypium raimondii]
           KJB09949.1 hypothetical protein B456_001G177100
           [Gossypium raimondii]
          Length = 275

 Score =  262 bits (669), Expect = 1e-82
 Identities = 138/279 (49%), Positives = 172/279 (61%), Gaps = 15/279 (5%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LIN+I   GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILINYINLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNF++EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFSREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKPNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
            ++             N+++ KD  +  Q  +       N  D     P E+ SE S +T
Sbjct: 121 GSIEN-----KRQPKSNVDSSKDIKKEQQEPMNFLSPAVNAADNRPASPPESSSEVSTVT 175

Query: 385 TMTTSGCSASTTESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFPESQFAL-PSYM---- 227
           T   +          +++V  ID+ FWS+V S D  ++  + Q   S   L P Y+    
Sbjct: 176 TSENNSNMCMNKIETQEYVSEIDENFWSEVLSADNSSIASDLQVAGSDQQLHPQYLPSSP 235

Query: 226 --------DVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
                   D       +D  MDFWYN+F + GDL EL E
Sbjct: 236 LPKLETINDYGSNLYDTDTNMDFWYNLFARVGDLSELPE 274


>XP_017640194.1 PREDICTED: myb-related protein Myb4-like [Gossypium arboreum]
           KHG21229.1 Myb-related Myb4 [Gossypium arboreum]
          Length = 275

 Score =  262 bits (669), Expect = 1e-82
 Identities = 138/279 (49%), Positives = 171/279 (61%), Gaps = 15/279 (5%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LIN+I   GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILINYINLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFTREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKPNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
            ++             N+++ KD  +  Q  +          D     P E+ SE S +T
Sbjct: 121 GSIDN-----KGQPKSNVDSSKDIKKEQQEPMNFLSPAVTAADNRPASPPESSSEVSTVT 175

Query: 385 TMTTSGCSASTTESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFPESQFAL-PSYM---- 227
           T   +          +++V  ID+ FWS+V S D  ++  + Q   S   L P Y+    
Sbjct: 176 TSENNSNMCMNKIETQEYVSEIDENFWSEVLSADNSSMASDFQVAGSDQQLHPQYLPSSP 235

Query: 226 --------DVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
                   D       +D  MDFWYN+F + GDL EL E
Sbjct: 236 LPKLETTNDYGSNLYDTDTNMDFWYNLFARVGDLSELPE 274


>XP_009629052.1 PREDICTED: myb-related protein Myb4-like [Nicotiana
           tomentosiformis] XP_016490215.1 PREDICTED: myb-related
           protein Myb4-like [Nicotiana tabacum] BAA88221.1
           myb-related transcription factor LBM1 [Nicotiana
           tabacum]
          Length = 281

 Score =  261 bits (666), Expect = 4e-82
 Identities = 147/291 (50%), Positives = 184/291 (63%), Gaps = 27/291 (9%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+L+++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILVSYIQTNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP------PQEAKS 404
              P+           N+++K  ++     T    DN  N +++ NGP      PQ + S
Sbjct: 119 YQPPQ---NSKRHSKNNLDSKAPST---SQTFNNSDNFSNIQEDINGPVTGPNSPQRSSS 172

Query: 403 E-------NSAMTTMTTSGCS----ASTTESMEKFV--IDDEFWSD--------VFSGDC 287
           E       ++AMTT+T    +        +S E F+  ID+ FW+D         F  + 
Sbjct: 173 EMSTVTVDSTAMTTITIDDQNMFKQLDEMDSSENFIPEIDESFWTDDLSTSDNSTFGMEG 232

Query: 286 PNLDVNHQFPESQFALPSYMDVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
              ++  QFP S     S MD+V      + +MDFWYNVF K GDL +L E
Sbjct: 233 TGGELQVQFPFSSVKQES-MDMV--GAKLEDDMDFWYNVFIKSGDLLDLPE 280


>XP_007050304.2 PREDICTED: myb-related protein Myb4 [Theobroma cacao]
          Length = 268

 Score =  258 bits (659), Expect = 3e-81
 Identities = 141/278 (50%), Positives = 175/278 (62%), Gaps = 17/278 (6%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+LIN+IK  GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILINYIKLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFTREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKQNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNG----PPQEAKSEN 398
            +             + I++ KD  +      QE  N  +   ++ G     P E+ SE 
Sbjct: 121 GS--------NDNKRQGIDSSKDIKKE-----QEPVNFLSPVTDSPGCRPVSPPESSSEV 167

Query: 397 SAMTTMTTSGCSASTTESMEKF-VIDDEFWSDVFSGDCPNLDVNHQFPESQFALPSYMDV 221
           S +TT   +       E+ + F  ID+ FWS+V S D  ++  + Q   S    P Y+ +
Sbjct: 168 STVTTSENNSNMCMKIETHQDFSEIDENFWSEVLSADNSSMASDFQVVGSDLQ-PQYLPI 226

Query: 220 ---VEFDP---------SSDPEMDFWYNVFTKEGDLQE 143
                F+P          SD  MDFWYN+FT+ GDL E
Sbjct: 227 SPLATFEPVNDYGSNLYVSDTNMDFWYNLFTRAGDLPE 264


>XP_004247733.1 PREDICTED: myb-related protein Myb4-like [Solanum lycopersicum]
          Length = 268

 Score =  258 bits (658), Expect = 4e-81
 Identities = 147/282 (52%), Positives = 177/282 (62%), Gaps = 18/282 (6%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+LI++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILISYIQSNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPL--QSTLQEGDNLQNKKDENNG------PPQEA 410
              P+                KD+  P   Q  L+  +N  N K++  G       PQ +
Sbjct: 119 YQPPQSSKRH--------SKNKDSKAPCTSQIALKSSNNFSNIKEDGPGLGSGPNSPQLS 170

Query: 409 KSENSAMT------TMTTSGCSASTTESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFPE 254
            SE S +T      TM  S  S    +S E F+  ID+ FW+D  S      ++  QFP 
Sbjct: 171 SSEMSTVTADSLAVTMDISN-SNDQIDSSENFIPEIDESFWTDGLSTSGGGEELQVQFPF 229

Query: 253 SQFALPSYMDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
                    + VE D  +  + +MDFWY+VF K GDL EL E
Sbjct: 230 HDMK----QENVEKDVGAKLEDDMDFWYSVFIKSGDLLELPE 267


>XP_015086701.1 PREDICTED: myb-related protein Myb4-like [Solanum pennellii]
          Length = 269

 Score =  258 bits (658), Expect = 4e-81
 Identities = 148/283 (52%), Positives = 177/283 (62%), Gaps = 19/283 (6%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+LI++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILISYIQSNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPL--QSTLQEGDNLQNKKDENNG--------PPQ 416
              P+                KD+  P   Q  L+  +N  N K++  G         PQ
Sbjct: 119 YQPPQSSKRH--------SKNKDSKAPCTSQIALKSSNNFSNIKEDGPGLGLGSGPNSPQ 170

Query: 415 EAKSENSAMTT---MTTSGCSAST--TESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFP 257
            + SE S +T      T G S S    +S E F+  ID+ FW+D  S      ++  QFP
Sbjct: 171 LSSSEMSTVTADSLAVTMGISNSNDQMDSSENFIPEIDESFWTDDLS-TSGGEELQVQFP 229

Query: 256 ESQFALPSYMDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
                     + VE D  +  + +MDFWYNVF K GDL EL E
Sbjct: 230 FHDMK----QEDVEMDVGAKLEDDMDFWYNVFIKSGDLLELPE 268


>EOX94461.1 Myb domain protein 13, putative [Theobroma cacao]
          Length = 268

 Score =  257 bits (657), Expect = 6e-81
 Identities = 141/278 (50%), Positives = 175/278 (62%), Gaps = 17/278 (6%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+LIN+IK  GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILINYIKLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   +
Sbjct: 61  LRPDIKRGNFTREEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKQNH 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNG----PPQEAKSEN 398
            +              N     D+S+ ++   QE  N  +   ++ G     P E+ SE 
Sbjct: 121 GS------------NDNKRQGIDSSQDIKKE-QEPVNFLSPVTDSPGCRPVSPPESSSEV 167

Query: 397 SAMTTMTTSGCSASTTESMEKF-VIDDEFWSDVFSGDCPNLDVNHQFPESQFALPSYMDV 221
           S +TT   +       E+ + F  ID+ FWS+V S D  ++  + Q   S    P Y+ +
Sbjct: 168 STVTTSENNSNMCMKIETHQDFSEIDENFWSEVLSADNSSMASDFQVVGSDLQ-PQYLPI 226

Query: 220 ---VEFDP---------SSDPEMDFWYNVFTKEGDLQE 143
                F+P          SD  MDFWYN+FT+ GDL E
Sbjct: 227 SPLATFEPVNDYGSNLYVSDTNMDFWYNLFTRAGDLPE 264


>OMP03097.1 hypothetical protein CCACVL1_02568 [Corchorus capsularis]
          Length = 306

 Score =  257 bits (657), Expect = 2e-80
 Identities = 149/308 (48%), Positives = 184/308 (59%), Gaps = 46/308 (14%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+LIN+I + GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILINYINQFGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL   N
Sbjct: 61  LRPDIKRGNFTQEEEDTIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRLKQYN 120

Query: 565 NA-------LPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGP----- 422
           N         P+          +   AK + S    S  +  +N++ ++D  N P     
Sbjct: 121 NGGSTSNENNPKKRQKTATTVAEAAAAKGNDS----SKDKNKNNIKKEQDPVNCPNSPAV 176

Query: 421 --PQEAKSENSAMTTMTTSGCSASTTESM-----------EKF-VIDDEFWSDVFSGDCP 284
             PQE+ S   +  T T+   S+S   +M           E+F  ID+ FWS+V S D  
Sbjct: 177 SSPQESLSSEVSTVTTTSENHSSSNNSNMMSTKMETQIEDEEFSEIDENFWSEVLSADNS 236

Query: 283 N------------LDVNHQ-FPESQFALPSYMDV-------VEFDPSSDPEMDFWYNVFT 164
           +               +H  FP S  A  +   V       + FD + D  MDFWYN+FT
Sbjct: 237 SEFQVVASSDQLQAQTDHDYFPSSPLAAATLEPVNNDFGSNILFD-NDDTNMDFWYNLFT 295

Query: 163 KEGDLQEL 140
           + GDL EL
Sbjct: 296 RAGDLPEL 303


>XP_002302045.1 GmMYB29 family protein [Populus trichocarpa] EEE81318.1 GmMYB29
           family protein [Populus trichocarpa]
          Length = 272

 Score =  255 bits (652), Expect = 4e-80
 Identities = 137/273 (50%), Positives = 177/273 (64%), Gaps = 9/273 (3%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL RGPWT EED++LIN I+  GHGNWRALPK+AGLLRCGKSCRLRW NY
Sbjct: 1   MVRAPCCEKMGLKRGPWTAEEDRILINHIQLYGHGNWRALPKQAGLLRCGKSCRLRWINY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNF++EEED II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL ++N
Sbjct: 61  LRPDIKRGNFSREEEDTIIKLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL-EQN 119

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
           +  PE           N E K    E + S+          K+     PQ+  S+ S++T
Sbjct: 120 HFAPEINGRSVDVSRFNHELKTG-PEIVSSSNVVAAGCDQSKEHRPISPQQCSSDVSSVT 178

Query: 385 TMTTSGCSASTTESMEKFV-IDDEFWSDVFSGDCPNLDVNHQF----PESQFAL-PSYMD 224
           T+ TS    +     + F+ +D+ FWS+V S D  ++  ++      P+ QF   P  +D
Sbjct: 179 TVDTSNDMCTKVAESDDFLEMDEIFWSEVLSADNSSMMSDYSAISTEPQLQFPFSPLIID 238

Query: 223 VVEFDPSSD---PEMDFWYNVFTKEGDLQELLE 134
           V +   ++      MDFW+N+FT+ G+L EL E
Sbjct: 239 VEQVQATNSNMYDGMDFWHNLFTRAGELPELTE 271


>CAA78387.1 protein 2 [Petunia x hybrida]
          Length = 280

 Score =  255 bits (652), Expect = 5e-80
 Identities = 143/284 (50%), Positives = 180/284 (63%), Gaps = 20/284 (7%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+L+++I++NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILVSYIEKNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDTIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAK-KDTSEPLQSTLQEGDNLQNKKDENNGP--PQEAKSENS 395
              P+           N ++K   TS+ L ++       +N  +   GP  PQ + SE+S
Sbjct: 119 YQPPQ---NSKRHSKNNHDSKAPSTSKMLDNSESFSTIQENINEPMTGPNSPQRSSSESS 175

Query: 394 AMTTMTTSGCSAST----------TESMEKF-VIDDEFWS-DVFSGDCPNLDVNHQFPES 251
            +T  + +    +            +S E F  ID+ FW+ D+  GD  +LD+     +S
Sbjct: 176 TVTADSLAATDVTNDDQTFIKHEEMDSYENFPEIDESFWTEDLSMGDNLDLDMEVAGEKS 235

Query: 250 QFALPSYMDVVE-----FDPSSDPEMDFWYNVFTKEGDLQELLE 134
           Q   P   D+ E          + +MDFWYNVF K  DL +L E
Sbjct: 236 QVQFPYSHDMKEQSVDMVGAKLEDDMDFWYNVFIKAEDLLDLPE 279


>XP_006354484.1 PREDICTED: myb-related protein Myb4-like [Solanum tuberosum]
          Length = 268

 Score =  254 bits (650), Expect = 7e-80
 Identities = 146/281 (51%), Positives = 178/281 (63%), Gaps = 17/281 (6%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPWT EEDQ+LI++I+ NGHGNWRALPK AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWTPEEDQILISYIQSNGHGNWRALPKLAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT+EEED+II LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKKRL  KN
Sbjct: 61  LRPDIKRGNFTREEEDSIIQLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKRL--KN 118

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPL--QSTLQEGDNLQNKKDENNG------PPQEA 410
              P+          ++ +   D+  P   Q  L+  +N  N + +  G       PQ +
Sbjct: 119 YQPPQ-------NSKRHSKNNNDSKAPCTSQIALKSSNNFSNIQQDGPGLGSGPNSPQLS 171

Query: 409 KSENSAMTT---MTTSGCS--ASTTESMEKFV--IDDEFWSDVFSGDCPNLDVNHQFPES 251
            SE S +T      T G S  +   +S E F+  ID+ FW+D  S      ++  QFP  
Sbjct: 172 SSEMSTVTADSLAVTMGISNGSDQMDSSENFIPEIDESFWTDDLS-TSGGEELQVQFPFH 230

Query: 250 QFALPSYMDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
                S    VE D  +  + +MDFWYNVF K GDL EL E
Sbjct: 231 DMKQES----VEMDVGAKLEDDMDFWYNVFIKSGDLLELPE 267


>CDY35519.1 BnaA01g23930D [Brassica napus]
          Length = 251

 Score =  253 bits (645), Expect = 2e-79
 Identities = 135/272 (49%), Positives = 171/272 (62%), Gaps = 6/272 (2%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GLNRGPWT EEDQ+L++FI ++GH NWRALPK+AGLLRCGKSCRLRW NY
Sbjct: 1   MGRAPCCEKMGLNRGPWTPEEDQILVSFIHQHGHSNWRALPKQAGLLRCGKSCRLRWMNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           L+PDI RGNFT+ EED IISLH+ LGN+WS IAAKLPGRTDNEIKNVWHTHLKKRL D  
Sbjct: 61  LKPDIKRGNFTQAEEDAIISLHQILGNRWSAIAAKLPGRTDNEIKNVWHTHLKKRLEDYQ 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENNGPPQEAKSENSAMT 386
              P+                  TS   +ST  +  ++  K        + A S N +  
Sbjct: 121 PTKPK------------------TSNKKKSTKPKSQSVPAKSKSTKTESELASSSNPSFE 162

Query: 385 TMTTSGCSASTTESMEKFV-----IDDEFWSD-VFSGDCPNLDVNHQFPESQFALPSYMD 224
           ++ ++  SAS   S E F      ID+ FW++ ++S D  N   N    + +F    ++D
Sbjct: 163 SLFSASPSASDEVSSETFTSHEEDIDESFWNETLYSQDEENHTSNLDELQQEF---QHLD 219

Query: 223 VVEFDPSSDPEMDFWYNVFTKEGDLQELLESL 128
            V  +   D EMDFW++V  + G  Q+LL  L
Sbjct: 220 SVGNEMIFDSEMDFWFDVLPRSGGEQDLLAGL 251


>NP_001268132.1 R2R3 Myb14 transcription factor [Vitis vinifera] ABW34392.1 R2R3
           Myb14 transcription factor [Vitis vinifera]
          Length = 272

 Score =  252 bits (644), Expect = 6e-79
 Identities = 144/287 (50%), Positives = 181/287 (63%), Gaps = 23/287 (8%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           MGRAPCCEK+GL +GPWT EEDQ+L+N+I   GHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MGRAPCCEKMGLKKGPWTPEEDQILVNYIHLYGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNFT EEE+ II LH+ LGN+WS IAAKLPGRTDNEIKNVWHTHLKKRL   N
Sbjct: 61  LRPDIKRGNFTSEEEETIIELHERLGNRWSAIAAKLPGRTDNEIKNVWHTHLKKRL-KHN 119

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEG--DNLQNKKDENNG-----PPQEAK 407
           +A P                K+ + +  Q   Q+   ++  N + E+ G      PQ + 
Sbjct: 120 HATP--------------PPKRHSLDASQVEKQQNPINSATNSRSESLGYGPVLSPQPSF 165

Query: 406 SENSAMTTMTTSGCSASTT---------ESMEKF-VIDDEFWSDVF----SGDCPNLDVN 269
           S+ S+  T TT+  +A+ +         +S E F  +D+ FWS+V     SG   +L   
Sbjct: 166 SDISSAATTTTTTTTATMSDITTPCIKVDSPEDFPEMDENFWSEVLSSNKSGAAGDLPGA 225

Query: 268 HQFPESQFALPSYMDVVEFDPSS--DPEMDFWYNVFTKEGDLQELLE 134
              P+ QF   S   V+   P S  D +M+FWYN+FT+ G+L EL E
Sbjct: 226 ASGPQLQFPF-SPRAVIGSSPYSTYDMDMEFWYNIFTRSGELHELSE 271


>AKV71950.1 R2R3-MYB protein [Rehmannia glutinosa]
          Length = 251

 Score =  251 bits (642), Expect = 6e-79
 Identities = 137/273 (50%), Positives = 176/273 (64%), Gaps = 9/273 (3%)
 Frame = -3

Query: 925 MGRAPCCEKVGLNRGPWTKEEDQMLINFIKENGHGNWRALPKKAGLLRCGKSCRLRWTNY 746
           M RAPCCEK+GL +GPW+ EEDQ+L++FI++NGHGNWRALPK+AGLLRCGKSCRLRWTNY
Sbjct: 1   MVRAPCCEKMGLKKGPWSPEEDQILVSFIQQNGHGNWRALPKQAGLLRCGKSCRLRWTNY 60

Query: 745 LRPDIIRGNFTKEEEDNIISLHKELGNKWSIIAAKLPGRTDNEIKNVWHTHLKKRLVDKN 566
           LRPDI RGNF+KEEE+ II+LH+ LGN+WS IAA+LPGRTDNEIKNVWHTHLKK+L    
Sbjct: 61  LRPDIKRGNFSKEEEETIINLHEMLGNRWSAIAARLPGRTDNEIKNVWHTHLKKKLKIHQ 120

Query: 565 NALPEXXXXXXXXXXKNIEAKKDTSEPLQSTLQEGDNLQNKKDENN--GPPQEAKSENSA 392
           ++                ++K+ +  P+     +   L+N   + N    PQ+  SE S+
Sbjct: 121 SSQ---------------DSKRHSINPIPKC--DPKVLENSNSDQNIVTSPQQCSSEMSS 163

Query: 391 MTTMTTSGCSASTTE---SMEKF-VIDDEFWSDVFSGD---CPNLDVNHQFPESQFALPS 233
           +T  +         E   S E F  ID+ FWS+  S D   CP+ D      E +F    
Sbjct: 164 VTDSSIEKKEVKNEEMDYSSEYFPQIDESFWSEELSKDDVYCPSADKTED--EDRF---P 218

Query: 232 YMDVVEFDPSSDPEMDFWYNVFTKEGDLQELLE 134
           +M  VE D  SD  MDFWYN+F + GD+ +L E
Sbjct: 219 FMQNVE-DARSDDSMDFWYNLFIRAGDMPDLPE 250