BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00010557
         (1754 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CDO99625.1 unnamed protein product [Coffea canephora]                 605   0.0  
XP_012849260.1 PREDICTED: sphingosine kinase 1 [Erythranthe gutt...   605   0.0  
XP_019162411.1 PREDICTED: sphingosine kinase 1 isoform X1 [Ipomo...   601   0.0  
XP_012091503.1 PREDICTED: sphingosine kinase 1 isoform X1 [Jatro...   600   0.0  
XP_011096211.1 PREDICTED: sphingosine kinase 1 isoform X1 [Sesam...   595   0.0  
XP_009589611.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   593   0.0  
XP_016482935.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   588   0.0  
XP_009793048.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   588   0.0  
XP_002317243.1 diacylglycerol kinase family protein [Populus tri...   588   0.0  
XP_010092628.1 Sphingosine kinase 1 [Morus notabilis] EXB51817.1...   588   0.0  
OAY26099.1 hypothetical protein MANES_16G021200 [Manihot esculenta]   587   0.0  
XP_010057556.1 PREDICTED: sphingosine kinase 1 [Eucalyptus grand...   585   0.0  
XP_019258869.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   584   0.0  
XP_011043284.1 PREDICTED: sphingosine kinase 1 isoform X1 [Popul...   584   0.0  
XP_018851472.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   580   0.0  
XP_017978728.1 PREDICTED: sphingosine kinase 1 isoform X1 [Theob...   579   0.0  
GAV58854.1 DAGK_cat domain-containing protein [Cephalotus follic...   579   0.0  
XP_002524674.1 PREDICTED: sphingosine kinase 1 isoform X1 [Ricin...   578   0.0  
XP_019267491.1 PREDICTED: sphingosine kinase 1 isoform X1 [Nicot...   578   0.0  
XP_016477647.1 PREDICTED: sphingosine kinase 1-like isoform X1 [...   577   0.0  

>CDO99625.1 unnamed protein product [Coffea canephora]
          Length = 487

 Score =  605 bits (1559), Expect = 0.0
 Identities = 302/488 (61%), Positives = 368/488 (75%), Gaps = 4/488 (0%)
 Frame = +3

Query: 48   EEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIE 227
            +  + E + S RVK+NGV    TL+  G+L +    L ++KEIL F    SKI I+ ++E
Sbjct: 3    QSRAPEALFSDRVKINGVVTLMTLTVNGQLRWSDRCLVVEKEILGFVVNGSKIKIRTIVE 62

Query: 228  KGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRK 407
             GAGI CG +  A VRK F F+ ++ +  ++W   L ++I SLGRPKRLFV VNP+GG++
Sbjct: 63   AGAGICCGGNKGALVRKNFTFEPLSDDLLRLWCDNLQNYIDSLGRPKRLFVLVNPYGGKR 122

Query: 408  SATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXX 587
            SA+KIF   VKPLLEDANIEITVQET ++LHAKE+  +L+L KYDGIVCVS         
Sbjct: 123  SASKIFLDFVKPLLEDANIEITVQETQYRLHAKEIALSLELPKYDGIVCVSGDGILVEVI 182

Query: 588  XXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVA 767
                   DW+TAI  PLG+VPAGTGNGM KSLLDS GEPC+ + A L+II+GH+ SLDVA
Sbjct: 183  NGLLGRGDWKTAITMPLGVVPAGTGNGMAKSLLDSAGEPCTPANAVLSIIRGHRHSLDVA 242

Query: 768  TLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVP 947
            T+ QG+T +FSVLMLAWGLV DIDIESEKYRWMGS+R DFYA+QRIF LRKY G I FVP
Sbjct: 243  TVSQGQTKFFSVLMLAWGLVGDIDIESEKYRWMGSARIDFYAIQRIFCLRKYNGCIKFVP 302

Query: 948  APGYEPYGEPVDLGDQFLGEA----DSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEG 1115
            APGYE  GE   +  +  GE     D T KL    + GQ + YQG  +D K+LNWRK++G
Sbjct: 303  APGYEACGEVTSIECESTGEVIPSEDGTDKL---LVNGQQNGYQGADIDMKNLNWRKVDG 359

Query: 1116 PFISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPH 1295
            PF+S+WLHNVPWG EDT AAPDAKFSDG LDLI++KDC+RL LL LMTELNNGGHVKSPH
Sbjct: 360  PFVSIWLHNVPWGGEDTMAAPDAKFSDGYLDLIMVKDCTRLGLLRLMTELNNGGHVKSPH 419

Query: 1296 VIYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQG 1475
            V Y+KVKAFILEPGPR+D+ +KEGIIDVDGE+LASGKGTY+ +QKTL+AY KL I ++QG
Sbjct: 420  VQYLKVKAFILEPGPRNDDPSKEGIIDVDGELLASGKGTYRCDQKTLLAYEKLHINVEQG 479

Query: 1476 LATLFTPV 1499
            LATLF P+
Sbjct: 480  LATLFCPI 487


>XP_012849260.1 PREDICTED: sphingosine kinase 1 [Erythranthe guttata] EYU27907.1
            hypothetical protein MIMGU_mgv1a005065mg [Erythranthe
            guttata]
          Length = 498

 Score =  605 bits (1560), Expect = 0.0
 Identities = 293/478 (61%), Positives = 367/478 (76%)
 Frame = +3

Query: 63   EGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEKGAGI 242
            E ++S +V+VNG    ATLS GG L +    L + K++L F+ + S+I I+A++E  AG 
Sbjct: 8    EFILSEQVRVNGRITAATLSSGGELGWGDRRLDVKKQVLGFSVEGSEIKIRAVVEAAAGS 67

Query: 243  TCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATKI 422
             C       +RK F  + ++ ++ ++W+++L  ++ SLGRPKRLFVFVNP+GG+KSA+ I
Sbjct: 68   WCSGDKTKLIRKCFTLELLSIDSLRLWSQKLQEYLDSLGRPKRLFVFVNPYGGKKSASTI 127

Query: 423  FEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXXX 602
            F  DVKPLL+DA++E TVQET +QLHAKEV R+LDL+KYDGIVCVS              
Sbjct: 128  FVSDVKPLLDDASVEYTVQETKYQLHAKEVARSLDLAKYDGIVCVSGDGILVEVLNGLLI 187

Query: 603  XXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQG 782
              DWE AI+ PLG+VPAGTGNGMVKSLLDS GEPC+AS AT++II+GHK SLDVAT+ QG
Sbjct: 188  REDWENAIKIPLGVVPAGTGNGMVKSLLDSCGEPCAASNATMSIIRGHKRSLDVATISQG 247

Query: 783  ETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGYE 962
            +T +FS+LMLAWGLVADIDIESEKYRWMGS+R DFY LQRI  LRKY G I+FVPA G+E
Sbjct: 248  DTKFFSMLMLAWGLVADIDIESEKYRWMGSARLDFYGLQRILGLRKYNGSIIFVPASGFE 307

Query: 963  PYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISVWLHN 1142
             YGEP+D+  + + E +S  K ++G +  Q   YQGP VD K LNWRK+ GPF+S+WLHN
Sbjct: 308  SYGEPIDVEKEVIFEGESKPKSDDGPVTTQDYGYQGPKVDMKSLNWRKINGPFVSIWLHN 367

Query: 1143 VPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKAF 1322
            VPWG EDT AAPDA+FSDGCLDLI++KDC +L+LL LM  LN+G HVKSPHV Y+KVKAF
Sbjct: 368  VPWGGEDTMAAPDAQFSDGCLDLIMIKDCPKLSLLMLMNGLNDGSHVKSPHVSYLKVKAF 427

Query: 1323 ILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTP 1496
            IL+PGPR D+  K GIIDVDGEVLA GKG+YKS +KTLM+Y+KL I +DQGLATLF P
Sbjct: 428  ILQPGPRVDDQTKAGIIDVDGEVLARGKGSYKSEEKTLMSYDKLLIKVDQGLATLFAP 485


>XP_019162411.1 PREDICTED: sphingosine kinase 1 isoform X1 [Ipomoea nil]
          Length = 471

 Score =  601 bits (1549), Expect = 0.0
 Identities = 296/480 (61%), Positives = 371/480 (77%), Gaps = 1/480 (0%)
 Frame = +3

Query: 63   EGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEKGAGI 242
            E V+S RV++NG+E   TL+ GG+L +P   L I+K++L F+ + S I I+A++E  AGI
Sbjct: 5    ETVLSDRVRINGIETPVTLTAGGQLRWPARCLWIEKQVLGFSVEGSLIKIRAVVESEAGI 64

Query: 243  TCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATKI 422
             C  +  A +RKTF  +  ++E+ ++WT++L  FI SLGRPKRLF+F+NP+GG+KSA+KI
Sbjct: 65   CCCENKTALLRKTFTLEPFSEESFRLWTQKLQEFIDSLGRPKRLFIFLNPYGGKKSASKI 124

Query: 423  FEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXXX 602
            F  DVKPLLEDAN+E  +QET +QLHAKE++R LDLSKYDG+VCVS              
Sbjct: 125  FLDDVKPLLEDANVEYALQETKYQLHAKEIIRNLDLSKYDGVVCVSGDGILVEVVNGLLE 184

Query: 603  XXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQG 782
              DW+TAI+  LG++PAGTGNGMVKSLLDSVGEPCSAS ATLAII+GHK +LDVAT+ QG
Sbjct: 185  REDWKTAIKISLGVIPAGTGNGMVKSLLDSVGEPCSASNATLAIIRGHKRALDVATISQG 244

Query: 783  ETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGYE 962
            +T +FSVLMLAWGL+ADIDIESEKYRWMGS+R DFYALQR+F LR+Y G I FVPAPG E
Sbjct: 245  QTKFFSVLMLAWGLIADIDIESEKYRWMGSARIDFYALQRMFCLRRYNGCIRFVPAPGDE 304

Query: 963  PYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPI-VDPKDLNWRKLEGPFISVWLH 1139
             +GEP             T+ +   S  G  S Y GP  VD +  NWRK++GPF+SVWLH
Sbjct: 305  VHGEP-------------TEPMEVYSNGGPESGYSGPSDVDVQKSNWRKIDGPFVSVWLH 351

Query: 1140 NVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKA 1319
            NVPWGSEDT AAPDAKFSDG LDLI++KDC +LALLSLMT LNNGGHVKSP+V Y KVK 
Sbjct: 352  NVPWGSEDTMAAPDAKFSDGYLDLIVVKDCPKLALLSLMTGLNNGGHVKSPYVFYFKVKE 411

Query: 1320 FILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTPV 1499
            F+LEPG R+++ +K+GIIDVDGEV+A G+GTYK  ++TLMAY+KL I +DQGLAT+F+P+
Sbjct: 412  FVLEPGARAEDPSKDGIIDVDGEVVARGRGTYKCEERTLMAYDKLHIKMDQGLATIFSPI 471


>XP_012091503.1 PREDICTED: sphingosine kinase 1 isoform X1 [Jatropha curcas]
            KDP20892.1 hypothetical protein JCGZ_21363 [Jatropha
            curcas]
          Length = 490

 Score =  600 bits (1546), Expect = 0.0
 Identities = 301/485 (62%), Positives = 360/485 (74%), Gaps = 3/485 (0%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWS---LAIDKEILSFTTQASKIIIKAL 221
            E++ E ++S RV VNG     TL+  G+L +       L I+KE+L F  + +KI IKA+
Sbjct: 5    ESTPELIMSDRVTVNGTITELTLTADGKLQWTERGQRCLTIEKEVLGFVIEGAKIRIKAI 64

Query: 222  IEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGG 401
            ++ G GI CG S  A VRK FVF  + +++ ++W  RL  +I SLGRPKRLF+FVNPFGG
Sbjct: 65   VDSGDGICCGGSKGALVRKDFVFQSLHEDSPRVWCHRLRDYIDSLGRPKRLFIFVNPFGG 124

Query: 402  RKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXX 581
             KSATK+F   VKPLLEDA+I+ITVQET HQLHAKEV  TLDL+KYDGIVCVS       
Sbjct: 125  NKSATKVFLDVVKPLLEDADIQITVQETKHQLHAKEVASTLDLTKYDGIVCVSGDGILVE 184

Query: 582  XXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLD 761
                     DW  AI+ PLG+VPAGTGNGMVKSLLDSVGEPC  S A LAII+GHK SLD
Sbjct: 185  VVNGLLAREDWSDAIKVPLGMVPAGTGNGMVKSLLDSVGEPCKPSNAILAIIRGHKCSLD 244

Query: 762  VATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILF 941
            VAT+ QGET +FSVLML+WGLVAD+DIESEKYRWMGS R DFYALQRIF LR Y G I F
Sbjct: 245  VATILQGETKFFSVLMLSWGLVADVDIESEKYRWMGSIRLDFYALQRIFHLRHYNGSISF 304

Query: 942  VPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPF 1121
            VP PG+E YGEP +   +F G+ +      E  +K Q   YQG   D   L+WR + GPF
Sbjct: 305  VPGPGFETYGEPTNYNAEFAGKQNICNPSQEQPVKAQQRGYQGSDADLISLDWRTISGPF 364

Query: 1122 ISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVI 1301
            +S+WLHNVPWG ED  AAPDAKFSDG LDLIL+K+C +L LL LMTE+++GGHVKSP+VI
Sbjct: 365  VSIWLHNVPWGGEDIMAAPDAKFSDGYLDLILIKECPKLPLLKLMTEMSSGGHVKSPYVI 424

Query: 1302 YIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLA 1481
            YIKVKAFILEPGPR+++  K GIIDVDGEVLA G G+YK  +KTLM Y+KLQIT+D+GLA
Sbjct: 425  YIKVKAFILEPGPRAEDPTKGGIIDVDGEVLARGNGSYKCEEKTLMVYDKLQITVDKGLA 484

Query: 1482 TLFTP 1496
            TLF+P
Sbjct: 485  TLFSP 489


>XP_011096211.1 PREDICTED: sphingosine kinase 1 isoform X1 [Sesamum indicum]
          Length = 496

 Score =  595 bits (1535), Expect = 0.0
 Identities = 286/476 (60%), Positives = 361/476 (75%)
 Frame = +3

Query: 69   VVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEKGAGITC 248
            ++S +V+VNG   TAT S GG+L +PG  L + K++L F+ +  KI I+ ++E   G+ C
Sbjct: 20   LLSDQVRVNGRITTATFSAGGQLTWPGRRLEMGKQVLGFSVEGFKIKIRVIVEASPGLCC 79

Query: 249  GVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATKIFE 428
                   VRKTF  + ++ +  ++WT++   ++ SLGRPKRLF+FVNP+GG+KSA+KIF 
Sbjct: 80   FSCKTTLVRKTFTLEFLSDDCLRVWTQKFQEYLESLGRPKRLFIFVNPYGGKKSASKIFV 139

Query: 429  QDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXXXXX 608
             DVKPLL+DAN+E T+QET H+ HAKEVV++LDL+KYDGIVCVS                
Sbjct: 140  SDVKPLLDDANVEYTIQETKHRFHAKEVVQSLDLAKYDGIVCVSGDGILVEVLNGLLVRG 199

Query: 609  DWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQGET 788
            DW+ AIR PLG+VPAGTGNGMVKSLLDS G+PCS   ATLAII+GHK SLDVAT+ QG+T
Sbjct: 200  DWDIAIRIPLGVVPAGTGNGMVKSLLDSRGQPCSPFNATLAIIRGHKRSLDVATISQGDT 259

Query: 789  IYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGYEPY 968
             +FS+LMLAWGLVADIDIESE YRWMGS+R DFYALQRIF LR Y G I FVPAPGYE Y
Sbjct: 260  RFFSLLMLAWGLVADIDIESEIYRWMGSARLDFYALQRIFGLRNYNGSICFVPAPGYESY 319

Query: 969  GEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISVWLHNVP 1148
            GEP+DL  + + E +S    ++  +  +   Y+GP VD K LNWRK++GPF+S+WLHNVP
Sbjct: 320  GEPLDLEKEVIVEGESQMTSDDRPVSTREYGYRGPKVDIKSLNWRKIDGPFVSIWLHNVP 379

Query: 1149 WGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKAFIL 1328
            WG E+T AAPDA+FSDGCLDL+++KDC + +LL LMT LN+G HVKSPHV Y+KVKAFIL
Sbjct: 380  WGGENTLAAPDAEFSDGCLDLVMIKDCPKCSLLKLMTGLNDGSHVKSPHVSYLKVKAFIL 439

Query: 1329 EPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTP 1496
            +PG R+D+  K GIIDVDGEVLA G+G YK  +K+LM+Y KL I +DQGLATLF P
Sbjct: 440  QPGTRTDDPTKGGIIDVDGEVLARGRGAYKWEEKSLMSYGKLLIKVDQGLATLFAP 495


>XP_009589611.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Nicotiana
            tomentosiformis] XP_016445994.1 PREDICTED: sphingosine
            kinase 1-like isoform X1 [Nicotiana tabacum]
          Length = 477

 Score =  593 bits (1530), Expect = 0.0
 Identities = 293/477 (61%), Positives = 371/477 (77%)
 Frame = +3

Query: 69   VVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEKGAGITC 248
            V+S+RV++NG+ V  TL+ GG+L +P   L+I+K++L F  + S+I I A+IE GAGI C
Sbjct: 10   VLSNRVQINGISVPLTLTAGGQLRWPDRCLSIEKDVLGFIIEGSRIKINAVIESGAGICC 69

Query: 249  GVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATKIFE 428
            G    A +RKTF F+ +++++ ++W +RLH  + SLGRPKRLFV +NP+GG++SA+KIF 
Sbjct: 70   GGDGGAPLRKTFTFEPLSEDSLRLWKQRLHAVLDSLGRPKRLFVLLNPYGGKRSASKIFL 129

Query: 429  QDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXXXXX 608
             DVKPLL+DANI  T+QET ++LHAKEVV +LDL  YDG++CVS                
Sbjct: 130  DDVKPLLDDANIHYTLQETKYRLHAKEVVHSLDLLSYDGVLCVSGDGILVEVVNGLLERE 189

Query: 609  DWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQGET 788
            DW +AI+ PLG++PAGT NGM KSLLDSVGE C+A  ATLAII+GH  SLDVAT+ QG+ 
Sbjct: 190  DWSSAIKMPLGVIPAGTSNGMAKSLLDSVGESCTAFNATLAIIRGHTRSLDVATVSQGQK 249

Query: 789  IYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGYEPY 968
             +FSVLMLAWGLVADIDIESEKYRWMGS+R DFYA+QRIF LR+YTG I F+ APGYE +
Sbjct: 250  RFFSVLMLAWGLVADIDIESEKYRWMGSARMDFYAIQRIFRLRRYTGCIKFLAAPGYENF 309

Query: 969  GEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISVWLHNVP 1148
            GEP++L  +         +LN   +  Q + Y GP  D K  N RK+EGPF+S+WLHNVP
Sbjct: 310  GEPLELEGEI-------NELNSNRV--QQNCYCGPAFDMKGFN-RKIEGPFVSIWLHNVP 359

Query: 1149 WGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKAFIL 1328
            WG ED  AAPDAKFSDG LDL++MKDCS+L+LL+LM+ELN GGHV+SPHV+Y+KVKAF+L
Sbjct: 360  WGGEDVLAAPDAKFSDGYLDLVVMKDCSKLSLLTLMSELNKGGHVRSPHVLYLKVKAFVL 419

Query: 1329 EPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTPV 1499
            EPGPRSD+S KEGIIDVDGEVLA G+GTYKS Q+TLMAY+KL I +DQGLAT+F+PV
Sbjct: 420  EPGPRSDDSNKEGIIDVDGEVLARGRGTYKSEQRTLMAYDKLHIEVDQGLATVFSPV 476


>XP_016482935.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Nicotiana tabacum]
          Length = 481

 Score =  588 bits (1516), Expect = 0.0
 Identities = 291/484 (60%), Positives = 373/484 (77%), Gaps = 1/484 (0%)
 Frame = +3

Query: 48   EEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIE 227
            +EA S  V+S RV++NGV    TL+  G+L +  + L+I KE+L F  + SKI I+A+ E
Sbjct: 4    KEAGSSPVLSDRVRINGVVTPVTLTAEGKLRWREYCLSIKKEVLGFLIKGSKIKIRAVKE 63

Query: 228  KGAGITCGVSSQAHVRK-TFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGR 404
             G GI+CG ++ A VR+ +F F+ +T+++ ++W+++L  FI SLGRPKRLFVFVNP+GG+
Sbjct: 64   NGGGISCGGNTGATVRRRSFSFEPLTEDSLRLWSQKLQEFIDSLGRPKRLFVFVNPYGGK 123

Query: 405  KSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXX 584
            KSA+KIF   VKPLL+DA+I+ TVQET +QLHAKEV ++LD+ +YDG+VCVS        
Sbjct: 124  KSASKIFIDSVKPLLDDAHIDYTVQETKYQLHAKEVAKSLDILRYDGVVCVSGDGILVEV 183

Query: 585  XXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDV 764
                    DW++AI+ PLG++PAGTGNGM KSLLD+VG+ C+AS ATLAII+GHK SLDV
Sbjct: 184  VNGLLEREDWDSAIKMPLGVIPAGTGNGMAKSLLDAVGQSCTASNATLAIIRGHKQSLDV 243

Query: 765  ATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFV 944
            AT+ QG+  +FSVLMLAWGL+ADIDIESEKYRWMGS+R D+YA+QRIF LR+Y G I FV
Sbjct: 244  ATVSQGQNRFFSVLMLAWGLIADIDIESEKYRWMGSARIDYYAIQRIFRLRRYNGCIKFV 303

Query: 945  PAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFI 1124
             APGYE +GEP+D+  + + +  S    ++G        Y GP    KD N RK+EGPF+
Sbjct: 304  AAPGYETFGEPIDVEGETIDDVKSNFVQHKG--------YCGPTFHMKDFN-RKIEGPFV 354

Query: 1125 SVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIY 1304
            S+WLHNVPWG ED  AAPDAKFSDG LDL+++KDC +L LLSLMTELN GGHV+SP+V+Y
Sbjct: 355  SIWLHNVPWGGEDALAAPDAKFSDGYLDLVVIKDCPKLTLLSLMTELNKGGHVRSPYVLY 414

Query: 1305 IKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLAT 1484
             KVKAF+LEPG R D+ +KEGIIDVDGEVLA GKGTYKS+QKTLM YNKL I +DQGLAT
Sbjct: 415  FKVKAFVLEPGSRLDDPSKEGIIDVDGEVLARGKGTYKSDQKTLMTYNKLHIKVDQGLAT 474

Query: 1485 LFTP 1496
            +F+P
Sbjct: 475  VFSP 478


>XP_009793048.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Nicotiana
            sylvestris]
          Length = 481

 Score =  588 bits (1516), Expect = 0.0
 Identities = 291/484 (60%), Positives = 373/484 (77%), Gaps = 1/484 (0%)
 Frame = +3

Query: 48   EEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIE 227
            +EA S  V+S RV++NGV    TL+  G+L +  + L+I KE+L F  + SKI I+A+ E
Sbjct: 4    KEAGSSPVLSDRVRINGVVTPVTLTAEGKLRWREYCLSIKKEVLGFLIKGSKIKIRAVKE 63

Query: 228  KGAGITCGVSSQAHVRK-TFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGR 404
             G GI+CG ++ A VR+ +F F+ +T+++ ++W+++L  FI SLGRPKRLFVFVNP+GG+
Sbjct: 64   NGGGISCGGNTGATVRRRSFSFEPLTEDSLRLWSQKLQEFIDSLGRPKRLFVFVNPYGGK 123

Query: 405  KSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXX 584
            KSA+KIF   VKPLL+DA+I+ TVQET +QLHAKEV ++LD+ +YDG+VCVS        
Sbjct: 124  KSASKIFIDSVKPLLDDAHIDYTVQETKYQLHAKEVAKSLDILRYDGVVCVSGDGILVEV 183

Query: 585  XXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDV 764
                    DW++AI+ PLG++PAGTGNGM KSLLD+VG+ C+AS ATLAII+GHK SLDV
Sbjct: 184  VNGLLEREDWDSAIKMPLGVIPAGTGNGMAKSLLDAVGQSCTASNATLAIIRGHKQSLDV 243

Query: 765  ATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFV 944
            AT+ QG+  +FSVLMLAWGL+ADIDIESEKYRWMGS+R D+YA+QRIF LR+Y G I FV
Sbjct: 244  ATVSQGQNRFFSVLMLAWGLIADIDIESEKYRWMGSARIDYYAIQRIFRLRRYNGCIKFV 303

Query: 945  PAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFI 1124
             APGYE +GEP+D+  + + +  S    ++G        Y GP    KD N RK+EGPF+
Sbjct: 304  AAPGYETFGEPIDVEGETIDDVKSNFVQHKG--------YCGPTFHMKDFN-RKIEGPFV 354

Query: 1125 SVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIY 1304
            S+WLHNVPWG ED  AAPDAKFSDG LDL+++KDC +L LLSLMTELN GGHV+SP+V+Y
Sbjct: 355  SIWLHNVPWGGEDALAAPDAKFSDGYLDLVVIKDCPKLTLLSLMTELNKGGHVRSPYVLY 414

Query: 1305 IKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLAT 1484
             KVKAF+LEPG R D+ +KEGIIDVDGEVLA GKGTYKS+QKTLM YNKL I +DQGLAT
Sbjct: 415  FKVKAFVLEPGSRLDDPSKEGIIDVDGEVLARGKGTYKSDQKTLMTYNKLHIRVDQGLAT 474

Query: 1485 LFTP 1496
            +F+P
Sbjct: 475  VFSP 478


>XP_002317243.1 diacylglycerol kinase family protein [Populus trichocarpa] EEE97855.1
            diacylglycerol kinase family protein [Populus
            trichocarpa]
          Length = 494

 Score =  588 bits (1517), Expect = 0.0
 Identities = 299/483 (61%), Positives = 358/483 (74%), Gaps = 6/483 (1%)
 Frame = +3

Query: 69   VVSSRVKVNGVEVTATLSPGGRLNFPG----WSLAIDKEILSFTTQASKIIIKALIEKGA 236
            ++S +VKVNG     TL+  G+L +       SL ++KE+L FT Q SKIIIKA+ EK  
Sbjct: 10   IISDQVKVNGNLTPLTLTTEGKLQWTTEKGQHSLTVEKEVLGFTIQGSKIIIKAVTEKRD 69

Query: 237  GITCGVSSQ--AHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKS 410
            GI C  S+   A  RK FVF+ ++ E+  +W + L  +I SLGRPKRLF+FVNPFGG+KS
Sbjct: 70   GIFCSASAGGGALARKDFVFEPLSDESLHLWCQHLRDYINSLGRPKRLFIFVNPFGGKKS 129

Query: 411  ATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXX 590
            A KI+   VKPLLEDA+++ITVQET +QLHAKEVVR +DL+KYDGIVCVS          
Sbjct: 130  ALKIYFDVVKPLLEDADVQITVQETKYQLHAKEVVRAMDLTKYDGIVCVSGDGILVEVVN 189

Query: 591  XXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVAT 770
                  DW  AI+ PLG VPAGTGNGMVKS LDS GEPC+AS A +AII+GHK SLDVAT
Sbjct: 190  GLLEREDWNAAIKMPLGTVPAGTGNGMVKSTLDSAGEPCTASNAVVAIIRGHKCSLDVAT 249

Query: 771  LRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPA 950
            + QG+T +FSVLM AWGLVADIDIESEKYRWMGS+R DFY LQR+  LR+Y+GRI FVPA
Sbjct: 250  ILQGDTKFFSVLMFAWGLVADIDIESEKYRWMGSARLDFYGLQRMLCLRQYSGRISFVPA 309

Query: 951  PGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISV 1130
            PG+E YGEP     +F     S     E  +K +   YQGP VD  +L WR + GPFISV
Sbjct: 310  PGFEAYGEPTRYNGEFTSTQSSINPGQEQHVKAEQYSYQGPDVDLTNLEWRTINGPFISV 369

Query: 1131 WLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIK 1310
            WLHNVPWG E T AAPDAKF+DG LDLIL+KDC +L LL+LMT L++GGHVKSPHV+Y+K
Sbjct: 370  WLHNVPWGGEGTMAAPDAKFADGNLDLILIKDCPKLGLLALMTNLSDGGHVKSPHVMYLK 429

Query: 1311 VKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLF 1490
            VKAFILEPG R+ +  + GIID+DGEVLA G GTYK +QKTLM Y+KLQIT+DQGLATLF
Sbjct: 430  VKAFILEPGQRTKDPTRGGIIDIDGEVLARGNGTYKHDQKTLMTYDKLQITMDQGLATLF 489

Query: 1491 TPV 1499
             PV
Sbjct: 490  CPV 492


>XP_010092628.1 Sphingosine kinase 1 [Morus notabilis] EXB51817.1 Sphingosine kinase
            1 [Morus notabilis]
          Length = 492

 Score =  588 bits (1515), Expect = 0.0
 Identities = 301/492 (61%), Positives = 363/492 (73%), Gaps = 9/492 (1%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGW---SLAIDKEILSFTTQASKIIIKAL 221
            E S   V S RV VNGV    TLS  G + +      SL ++KE+L F T  SK+ I+ +
Sbjct: 7    EQSRRPVFSDRVAVNGVYTPLTLSADGVIRWSEGGQRSLTVEKEVLGFATDGSKLEIRTV 66

Query: 222  IEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGG 401
            +E G GI C  S  A VRK FVF   ++++ ++   +L  FI SLGRPKRL+VFVNPFGG
Sbjct: 67   VEGGGGILCFGSRGALVRKNFVFLFSSEDSQKLLCLKLREFIDSLGRPKRLYVFVNPFGG 126

Query: 402  RKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXX 581
            +KSA+KIF   VKPLLEDA+I++T+QET +QLHAKEV +T+DLSKYDGIVCVS       
Sbjct: 127  KKSASKIFSDQVKPLLEDADIQLTLQETKYQLHAKEVAKTMDLSKYDGIVCVSGDGILVE 186

Query: 582  XXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLD 761
                     DWETAI+TPLG+VPAGTGNGM KSLLDSVGEPC+   A LAII+GHK  LD
Sbjct: 187  IVNGLLERGDWETAIKTPLGVVPAGTGNGMAKSLLDSVGEPCAVFNAVLAIIRGHKRPLD 246

Query: 762  VATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILF 941
            V T+ QGET +FSVLMLAWGL+ADIDIESEKYRWMGS+R DFY LQR+FSLRKY GRI F
Sbjct: 247  VTTILQGETKFFSVLMLAWGLIADIDIESEKYRWMGSARLDFYGLQRVFSLRKYNGRISF 306

Query: 942  VPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGS------LKGQPSHYQGPIVDPKDLNWR 1103
            VPAPG+E YGEP         ++++T K N G       +K Q   YQGP  D   ++WR
Sbjct: 307  VPAPGFEGYGEPTSY------KSETTSKQNVGGPTEVEPVKLQRHGYQGPDSDLHIMDWR 360

Query: 1104 KLEGPFISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHV 1283
             + GPF+SVWLHNVPWGSE+T AAP AKFSDG +DLI+M+DC +LALLSLM ELNNG HV
Sbjct: 361  TINGPFVSVWLHNVPWGSENTMAAPQAKFSDGYIDLIIMRDCPKLALLSLMGELNNGNHV 420

Query: 1284 KSPHVIYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQIT 1463
             SP+V Y+KVKAFILEPGP +++  +EGIID DGEVLA GKGTYK +QKTLMAY+K Q+ 
Sbjct: 421  NSPYVTYLKVKAFILEPGPLAEDPTREGIIDSDGEVLARGKGTYKCDQKTLMAYDKFQLA 480

Query: 1464 IDQGLATLFTPV 1499
            +DQGLATLF+P+
Sbjct: 481  VDQGLATLFSPL 492


>OAY26099.1 hypothetical protein MANES_16G021200 [Manihot esculenta]
          Length = 490

 Score =  587 bits (1514), Expect = 0.0
 Identities = 293/486 (60%), Positives = 357/486 (73%), Gaps = 3/486 (0%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWS---LAIDKEILSFTTQASKIIIKAL 221
            E+S + ++S RV VNG     TL+  G+L +       L I+KE+L F  + ++I IKA+
Sbjct: 5    ESSPQSILSDRVTVNGTVTQLTLTADGKLRWTDRGQRCLTIEKEVLGFVIEGARIRIKAV 64

Query: 222  IEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGG 401
            ++    I C  S    VRK FVF  + ++  +IW + L  ++ SLGRPKRLFVFVNPFGG
Sbjct: 65   VDSEDSIFCAGSKGTLVRKDFVFHPLNEDQQRIWFQHLRDYLDSLGRPKRLFVFVNPFGG 124

Query: 402  RKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXX 581
            +KSA+K+F   VKPLLEDA+++ITVQET HQLHAKEV  TLDL+KYDGIVCVS       
Sbjct: 125  KKSASKVFRDVVKPLLEDADVQITVQETKHQLHAKEVANTLDLTKYDGIVCVSGDGILVE 184

Query: 582  XXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLD 761
                     DW  AI+ PLG+VPAGTGNGM KSLLDSVGEPC AS A LA+I+GHK SLD
Sbjct: 185  VVNGFLAREDWNDAIKMPLGMVPAGTGNGMAKSLLDSVGEPCEASNAILAVIRGHKRSLD 244

Query: 762  VATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILF 941
            VA + QGET +FSVLML+WGLVADIDIESEKYRWMG++R DFYA+QRI  LRKY GRI F
Sbjct: 245  VAAILQGETKFFSVLMLSWGLVADIDIESEKYRWMGTARLDFYAIQRIIHLRKYNGRISF 304

Query: 942  VPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPF 1121
            VPAPG+E YGEP +   +  G+   +    E  +K     YQGP VD  +L WR + GPF
Sbjct: 305  VPAPGFESYGEPSNYNGETTGKQSISISSQEQPVKIDQHGYQGPDVDLINLEWRTISGPF 364

Query: 1122 ISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVI 1301
            +S+WLHNVPWG ED  AAPDAKFSDG LDLIL+ +C +L+LL+L++EL  GGHVKSP+VI
Sbjct: 365  VSIWLHNVPWGGEDVMAAPDAKFSDGYLDLILIGECPKLSLLTLLSELKTGGHVKSPYVI 424

Query: 1302 YIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLA 1481
            Y+KVKAF+LEPGPR+D   KEGIIDVDGEVLA G G+YK  + TLMAY+KLQIT+D GLA
Sbjct: 425  YLKVKAFVLEPGPRADEPMKEGIIDVDGEVLARGNGSYKCERPTLMAYDKLQITVDPGLA 484

Query: 1482 TLFTPV 1499
            TLFTPV
Sbjct: 485  TLFTPV 490


>XP_010057556.1 PREDICTED: sphingosine kinase 1 [Eucalyptus grandis] KCW74745.1
            hypothetical protein EUGRSUZ_E03468 [Eucalyptus grandis]
          Length = 497

 Score =  585 bits (1509), Expect = 0.0
 Identities = 290/495 (58%), Positives = 361/495 (72%), Gaps = 7/495 (1%)
 Frame = +3

Query: 36   PPHMEEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGW---SLAIDKEILSFTTQASKI 206
            PP  +       +S RV + G     TL+  G L +PG    SL++++E+L    +  +I
Sbjct: 3    PPGADNPPPPPAISDRVLIGGTVDPVTLTADGSLVWPGGRHRSLSVEEEVLGVDAEGPRI 62

Query: 207  IIKALIEKGAGITC----GVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRL 374
             IKAL++ GAG       G      VRK  VF+ +++E+ ++W  +L   + SL RPK+L
Sbjct: 63   RIKALVDDGAGDRACWAGGKGGGGLVRKDVVFEPLSEESRRLWCDKLRGCLDSLDRPKKL 122

Query: 375  FVFVNPFGGRKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVC 554
            F+ +NPFGG+K A K     VKPLLEDA+I+  +QET HQ HAKEV R+LDLSKYDGIVC
Sbjct: 123  FILLNPFGGKKLAPKTLANVVKPLLEDASIQFDIQETQHQFHAKEVARSLDLSKYDGIVC 182

Query: 555  VSXXXXXXXXXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAI 734
            VS                DWE AI+ P+G+VPAGTGNGMVKSLLDSVGEPC+ASYATLAI
Sbjct: 183  VSGDGILVEVVNGLLEREDWEAAIKVPIGIVPAGTGNGMVKSLLDSVGEPCTASYATLAI 242

Query: 735  IQGHKTSLDVATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSL 914
            I+GHK SLDVA ++QG+  +FS+LML+WGLVADIDIESEKYRWMGS+R DFYALQRI  L
Sbjct: 243  IRGHKKSLDVAAIKQGDAKFFSILMLSWGLVADIDIESEKYRWMGSARLDFYALQRILHL 302

Query: 915  RKYTGRILFVPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDL 1094
            R+Y+GRI FVPAPGYE +GEP      F G+    +K    S K +   YQGP V  ++L
Sbjct: 303  RRYSGRISFVPAPGYETHGEPTSYSGNFGGKDIVFEKSERSSAKVEGQGYQGPDVKLEEL 362

Query: 1095 NWRKLEGPFISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNG 1274
            +WR ++GPF+S+WLHNVPWG ED  AAPDAKFSDGCLDLI ++DC +L+LLSLMTELNNG
Sbjct: 363  SWRSIDGPFVSIWLHNVPWGGEDIMAAPDAKFSDGCLDLIAIRDCPKLSLLSLMTELNNG 422

Query: 1275 GHVKSPHVIYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKL 1454
            GHVKSP+VIYIKVKAF+LEPGPR D+  KEGIID DGEVLA GKGT+KS  KT+MAY++L
Sbjct: 423  GHVKSPYVIYIKVKAFVLEPGPRVDDPTKEGIIDTDGEVLAKGKGTFKSEHKTIMAYDRL 482

Query: 1455 QITIDQGLATLFTPV 1499
             IT+DQGLAT+F+P+
Sbjct: 483  LITVDQGLATVFSPI 497


>XP_019258869.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Nicotiana attenuata]
            OIT40233.1 sphingosine kinase 1 [Nicotiana attenuata]
          Length = 481

 Score =  584 bits (1505), Expect = 0.0
 Identities = 291/484 (60%), Positives = 369/484 (76%), Gaps = 1/484 (0%)
 Frame = +3

Query: 48   EEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIE 227
            +EA S  V+S RV+ NGV  T TL+  G+L +    L+I KE+L F  + SKI I+A+ E
Sbjct: 4    KEAGSSPVLSDRVRFNGVVTTVTLTAEGKLRWRENCLSIKKEVLGFLIKGSKIKIRAVKE 63

Query: 228  KGAGITCGVSSQAHVRK-TFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGR 404
             G GI+CG ++ A VR+ +F F+ +T+++ ++W+++L  FI SLGRPKRLFVFVNP+GG+
Sbjct: 64   NGGGISCGGNTGATVRRRSFSFEPLTEDSLRLWSQKLQEFIDSLGRPKRLFVFVNPYGGK 123

Query: 405  KSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXX 584
            KSA+KIF   VKPLL+DANI+ TVQET +QLHAKEV ++LD+ +YDG+VCVS        
Sbjct: 124  KSASKIFIDSVKPLLDDANIDYTVQETKYQLHAKEVAKSLDILRYDGVVCVSGDGILVEV 183

Query: 585  XXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDV 764
                    DW++AI+ PLG++PAGTGNGM KSLLD+VG+ C+A  ATLAII+GHK SLDV
Sbjct: 184  VNGLLEREDWDSAIKMPLGVIPAGTGNGMAKSLLDAVGQSCTACNATLAIIRGHKQSLDV 243

Query: 765  ATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFV 944
            AT+ QG+  +FSVLMLAWGL+ADIDIESEKYRWMGS+R D+YA+QRIF LR+Y G I FV
Sbjct: 244  ATVSQGQNRFFSVLMLAWGLIADIDIESEKYRWMGSARIDYYAIQRIFRLRRYNGCIKFV 303

Query: 945  PAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFI 1124
              PGYE +GEP D+  + + +  S    ++G        Y GP    KD N RK+EGPF+
Sbjct: 304  AGPGYETFGEPTDVEGETIDDVKSNFVQHKG--------YCGPTFHMKDFN-RKIEGPFV 354

Query: 1125 SVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIY 1304
            S+WLHNVPWG ED  AAPDAKFSDG LDL+++KDC +L LLSLMTELN GGHV+SP+V+Y
Sbjct: 355  SIWLHNVPWGGEDALAAPDAKFSDGYLDLVVIKDCPKLTLLSLMTELNKGGHVRSPYVLY 414

Query: 1305 IKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLAT 1484
             KVKAF+LEPG R D+ +KEGIIDVDGEVLA GKGTYKS+QKTLM YNKL I +DQGLAT
Sbjct: 415  FKVKAFVLEPGSRLDDPSKEGIIDVDGEVLARGKGTYKSDQKTLMTYNKLHIKVDQGLAT 474

Query: 1485 LFTP 1496
            +F+P
Sbjct: 475  VFSP 478


>XP_011043284.1 PREDICTED: sphingosine kinase 1 isoform X1 [Populus euphratica]
          Length = 492

 Score =  584 bits (1505), Expect = 0.0
 Identities = 294/487 (60%), Positives = 355/487 (72%), Gaps = 4/487 (0%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPG----WSLAIDKEILSFTTQASKIIIKA 218
            +     ++S +VKVNG     TL+  G+L++       SL ++KE+L FT Q SKI+IKA
Sbjct: 4    QQEDSSIISDQVKVNGNLTPLTLTTEGKLHWTTEKGQHSLTVEKEVLGFTIQGSKILIKA 63

Query: 219  LIEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFG 398
            + EK  G        A  RK FVF+ ++ E+  +W + L  +I SLGRPKRLF+FVNPFG
Sbjct: 64   VTEKRDGWFASAGGGALARKNFVFEPLSDESLHLWCQHLRDYINSLGRPKRLFIFVNPFG 123

Query: 399  GRKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXX 578
            G+KSA KI+   VKPLLEDA+++ITVQET +QLHAKE+V  +DL+KYDGIVCVS      
Sbjct: 124  GKKSALKIYLDVVKPLLEDADVQITVQETKYQLHAKELVSAMDLTKYDGIVCVSGDGILV 183

Query: 579  XXXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSL 758
                      DW  AI+ PLG VPAGTGNGMVKS LDS GEPC+AS A +AII+GHK SL
Sbjct: 184  EVVNGLLEREDWNAAIKMPLGTVPAGTGNGMVKSTLDSAGEPCTASNAVVAIIRGHKCSL 243

Query: 759  DVATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRIL 938
            DVAT+ QG+T +FSVLMLAWGLVADIDIESEKYRWMGS+R DFY LQR+  LR+Y+GRI 
Sbjct: 244  DVATILQGDTKFFSVLMLAWGLVADIDIESEKYRWMGSARLDFYGLQRMLCLRQYSGRIS 303

Query: 939  FVPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGP 1118
            FVPAPG+E YGEP+    +F     S     E  +K +   YQGP VD  +L WR + GP
Sbjct: 304  FVPAPGFEGYGEPIKYNGEFTSVQSSINPGQEQHVKAEQYSYQGPDVDLTNLEWRTINGP 363

Query: 1119 FISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHV 1298
            FISVWLHNVPWG E T AAPDAKF+DG LDLIL+KDC +L LL+LMT L+NG HVKSPHV
Sbjct: 364  FISVWLHNVPWGGEGTMAAPDAKFADGNLDLILIKDCPKLGLLALMTNLSNGRHVKSPHV 423

Query: 1299 IYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGL 1478
            +Y+KVKAFILEPG R+ +  + GIID+DGEVLA G GTYK  QKTLM Y+KLQIT+DQGL
Sbjct: 424  MYLKVKAFILEPGQRTKDPTRGGIIDIDGEVLARGNGTYKHGQKTLMTYDKLQITVDQGL 483

Query: 1479 ATLFTPV 1499
            ATLF PV
Sbjct: 484  ATLFCPV 490


>XP_018851472.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Juglans regia]
          Length = 490

 Score =  580 bits (1495), Expect = 0.0
 Identities = 294/488 (60%), Positives = 364/488 (74%), Gaps = 3/488 (0%)
 Frame = +3

Query: 45   MEEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWS---LAIDKEILSFTTQASKIIIK 215
            M++  S  ++S RV VNG     TL+  GRL +   S   L ++KE+L F+ +  KI I+
Sbjct: 1    MDQPESLPILSDRVLVNGGVTPLTLTADGRLRWTERSQRSLKVEKEVLGFSAEGPKIRIR 60

Query: 216  ALIEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPF 395
            A++E G GI C  S++  VRK FVF  +++++  +W +RL  +I SLGRPK LFV +NPF
Sbjct: 61   AIVEGGGGIFCIGSARNLVRKDFVFQPLSEDSLVLWCQRLREYIDSLGRPKHLFVLLNPF 120

Query: 396  GGRKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXX 575
            GG+KSA+KIF   VK L EDA+I+IT+QET ++LHAKEV  +LDLSKYDGIVCVS     
Sbjct: 121  GGKKSASKIFVDHVKTLFEDADIQITLQETQYRLHAKEVANSLDLSKYDGIVCVSGDGIL 180

Query: 576  XXXXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTS 755
                       DW  AI+ P+G++PAGTGNGM KSLLDSVG PC+A  A LAII+GHK S
Sbjct: 181  VEVVNGLLGREDWGAAIKMPIGVIPAGTGNGMAKSLLDSVGAPCAAPNAVLAIIRGHKCS 240

Query: 756  LDVATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRI 935
            +DVAT+ QGE  +FSVLMLAWGLVADIDIESEKYR +GS+R DFYALQRI  LR+Y G I
Sbjct: 241  VDVATIVQGEAKFFSVLMLAWGLVADIDIESEKYRCLGSARLDFYALQRILHLRQYNGCI 300

Query: 936  LFVPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEG 1115
             FVP PG+E YGEP +   +  G+ +S     E   K Q   YQGP +D +DL+WR + G
Sbjct: 301  SFVPVPGFEAYGEPTNFHGKSTGKQNSHIPSEEDPAKAQQHGYQGPYIDLEDLDWRTING 360

Query: 1116 PFISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPH 1295
            PFISVWLHNVPWGSEDT AAPDAKFSDG LDLIL+++C +L LLSLMTEL +G HVKSP+
Sbjct: 361  PFISVWLHNVPWGSEDTMAAPDAKFSDGYLDLILIRNCPQLPLLSLMTELKSGNHVKSPY 420

Query: 1296 VIYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQG 1475
            V+Y+KVKAFILEPGP++ +  KEGIID DGEVLA GKGTYK +QKTLMAY+KLQIT+DQG
Sbjct: 421  VMYLKVKAFILEPGPQTQDPTKEGIIDSDGEVLARGKGTYKCDQKTLMAYDKLQITVDQG 480

Query: 1476 LATLFTPV 1499
            LATLF+P+
Sbjct: 481  LATLFSPI 488


>XP_017978728.1 PREDICTED: sphingosine kinase 1 isoform X1 [Theobroma cacao]
          Length = 489

 Score =  579 bits (1493), Expect = 0.0
 Identities = 290/480 (60%), Positives = 360/480 (75%), Gaps = 3/480 (0%)
 Frame = +3

Query: 69   VVSSRVKVNGVEVTATLSPGGRLNFPGWS---LAIDKEILSFTTQASKIIIKALIEKGAG 239
            ++S RV VNG  V  T +  G+L + G     L ++KE+L F  + ++I +K  +EKG G
Sbjct: 13   LLSDRVLVNGTVVPLTFTGDGKLRWTGKDQRCLTMEKEVLGFALEGTRIQVKYAVEKGDG 72

Query: 240  ITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATK 419
            I C  +    VR++FVF+ ++ ++ ++W+++L  +I SLGRPKRL VFVNP+GG+KSATK
Sbjct: 73   ICCLWNRGDLVRQSFVFEPLSDDSLRLWSQKLRDYIDSLGRPKRLLVFVNPYGGKKSATK 132

Query: 420  IFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXX 599
            IF +DVKP LEDA+++ITV ET HQLHAKEV +TLDLSKYDGIVCVS             
Sbjct: 133  IFSEDVKPYLEDADVQITVIETKHQLHAKEVAKTLDLSKYDGIVCVSGDGILVEVVNGLL 192

Query: 600  XXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQ 779
               DW  AI+ P+G+VPAGTGNGMVKSLLD+VG+PCS S A LA+I+GHK SLDVAT+ Q
Sbjct: 193  EREDWAAAIKIPIGMVPAGTGNGMVKSLLDAVGQPCSTSNAILAVIRGHKCSLDVATILQ 252

Query: 780  GETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGY 959
            GET +FSVLMLAWGL+ADIDIESEKYRWMGS+R DFYALQR+  LR Y G + FVPAPG+
Sbjct: 253  GETRFFSVLMLAWGLIADIDIESEKYRWMGSARLDFYALQRLLHLRHYNGCVSFVPAPGF 312

Query: 960  EPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISVWLH 1139
            E YGEP     +   E   T+   E S+K Q   Y G     ++ +WR + GPFISVWLH
Sbjct: 313  EDYGEPTGYHGEPTSEKSPTE---ENSVKTQRHGYHGSDFKLENKHWRTISGPFISVWLH 369

Query: 1140 NVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKA 1319
            NVPWGSED  AAP AKFSDGCLDLI+++D  +L LLSLM+++N+G HVKSPHV YIKVKA
Sbjct: 370  NVPWGSEDCMAAPKAKFSDGCLDLIIVRDSPKLPLLSLMSKMNDGSHVKSPHVTYIKVKA 429

Query: 1320 FILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTPV 1499
            F+LEPGPR ++ AKEGIID DGEVLA GKGTYK +QK LMAY+KLQ+T+DQGLATLF PV
Sbjct: 430  FVLEPGPRVEDPAKEGIIDSDGEVLARGKGTYKCDQKALMAYDKLQLTLDQGLATLFAPV 489


>GAV58854.1 DAGK_cat domain-containing protein [Cephalotus follicularis]
          Length = 491

 Score =  579 bits (1492), Expect = 0.0
 Identities = 292/491 (59%), Positives = 355/491 (72%), Gaps = 6/491 (1%)
 Frame = +3

Query: 45   MEEASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGW---SLAIDKEILSFTTQASKIIIK 215
            M+   ++ ++S RV VNG   T TL+  G   +      SL ++K++L F T+  KI IK
Sbjct: 1    MDRPENQLLISDRVLVNGTVTTLTLTGDGSFQWTERGQRSLRVEKQVLGFETEGCKIKIK 60

Query: 216  ALIEKGAGITC---GVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFV 386
             ++E G GI C    V   A VRK FVF+ +++++  +W  RL  +I SLGRPKRLF+FV
Sbjct: 61   TVVEGGDGICCVGTTVGGGALVRKDFVFEPLSQDSKTLWCNRLRDYIDSLGRPKRLFIFV 120

Query: 387  NPFGGRKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXX 566
            NPFGG++SA+K+F   V+P LEDA ++ TVQET +QLHAKEV   LDLS YDGIVCVS  
Sbjct: 121  NPFGGKRSASKVFLDHVRPYLEDAGVQFTVQETEYQLHAKEVTNRLDLSMYDGIVCVSGD 180

Query: 567  XXXXXXXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGH 746
                          DW TAI+ P+G+VPAGTGNGM KSLLDSVG+PCS S A LA+I+GH
Sbjct: 181  GILVEVVNGLLERDDWSTAIKKPIGIVPAGTGNGMAKSLLDSVGDPCSPSDAILAVIRGH 240

Query: 747  KTSLDVATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYT 926
            K SLDVAT+ Q ET +FSVLMLAWGLVADIDIESEKYRWMGS+R DFYALQR+  LR Y+
Sbjct: 241  KRSLDVATIVQRETKFFSVLMLAWGLVADIDIESEKYRWMGSARIDFYALQRMIHLRHYS 300

Query: 927  GRILFVPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRK 1106
            G I FVPAPG+E YG+      +F  E +      E S+K Q   YQGP V    L+WR 
Sbjct: 301  GHISFVPAPGFEDYGDQTSYNGEFASEQNIGSPSQEHSIKVQQYGYQGPDVKLDSLDWRT 360

Query: 1107 LEGPFISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVK 1286
            + GPF+ VWLHNVPWG E T AAPDAKFSDG LDLI+++DC +  LLSLM+ELNNGGHVK
Sbjct: 361  INGPFVCVWLHNVPWGGEQTMAAPDAKFSDGYLDLIIVRDCPKFELLSLMSELNNGGHVK 420

Query: 1287 SPHVIYIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITI 1466
            SP V Y+KVKAF+LEPGPR+ +  KEGIID DGEVLA GKGTY ++QKTLMAY+KLQIT+
Sbjct: 421  SPQVKYLKVKAFVLEPGPRTKDPTKEGIIDADGEVLARGKGTYNNDQKTLMAYDKLQITV 480

Query: 1467 DQGLATLFTPV 1499
            DQGLATLF PV
Sbjct: 481  DQGLATLFAPV 491


>XP_002524674.1 PREDICTED: sphingosine kinase 1 isoform X1 [Ricinus communis]
            EEF37693.1 Sphingosine kinase, putative [Ricinus
            communis]
          Length = 479

 Score =  578 bits (1490), Expect = 0.0
 Identities = 296/486 (60%), Positives = 360/486 (74%), Gaps = 3/486 (0%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWS---LAIDKEILSFTTQASKIIIKAL 221
            ++S + ++S +V VNG     TL+ GG L +       L ++KE+L F  + SKI +K +
Sbjct: 2    DSSPQPIISDQVIVNGATTLLTLTAGGILRWTDRGQRCLTVEKEVLGFVIEGSKIRVKTI 61

Query: 222  IEKGAGITCGVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGG 401
            I+ G GI C  +S A VRK FVF  +T+++ ++   RL  ++ SLGRPKRL VFVNPFGG
Sbjct: 62   IDNGDGICCAGNSGALVRKDFVFQPLTEDSQRVLCNRLRDYLDSLGRPKRLLVFVNPFGG 121

Query: 402  RKSATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXX 581
            ++SA+KIF   VKPLLEDA+++IT+QET HQLHAKEV  TLD+SKYDGIVCVS       
Sbjct: 122  KRSASKIFFDTVKPLLEDADVQITLQETKHQLHAKEVTSTLDISKYDGIVCVSGDGILVE 181

Query: 582  XXXXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLD 761
                     DW  AI+ PLG+VPAGT NGM KSLLDSVGEPC AS A LAII+GHK SLD
Sbjct: 182  VVNGLLAREDWRDAIKLPLGMVPAGTSNGMAKSLLDSVGEPCKASNAVLAIIRGHKCSLD 241

Query: 762  VATLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILF 941
            VAT+ QGET +FSVLML+WGLVADIDIESEKYRWMGS+R DFYA+QRIF LR Y G I F
Sbjct: 242  VATILQGETKFFSVLMLSWGLVADIDIESEKYRWMGSARIDFYAVQRIFHLRHYNGCISF 301

Query: 942  VPAPGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPF 1121
            VPAPG+E YG P          A+ST K  E  LK Q   YQGP V+  +L+WR + GPF
Sbjct: 302  VPAPGFETYGVPTSYN------AESTSK-QEQPLKTQHG-YQGPDVNLVNLDWRMISGPF 353

Query: 1122 ISVWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVI 1301
            +S+WLHNVPWG ED  AAPDAKFSDG LDLIL++ C +L+LL+LMT LNNG HVKSP+VI
Sbjct: 354  VSIWLHNVPWGGEDVMAAPDAKFSDGYLDLILIQQCPKLSLLALMTALNNGDHVKSPYVI 413

Query: 1302 YIKVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLA 1481
            Y+KVKAFILEPGPR+D+  K GIIDVDGEVLA G G+YK +QK+LM Y+KL + +DQGLA
Sbjct: 414  YLKVKAFILEPGPRTDDPTKGGIIDVDGEVLARGNGSYKCDQKSLMVYDKLHMMVDQGLA 473

Query: 1482 TLFTPV 1499
            TLF+PV
Sbjct: 474  TLFSPV 479


>XP_019267491.1 PREDICTED: sphingosine kinase 1 isoform X1 [Nicotiana attenuata]
          Length = 477

 Score =  578 bits (1489), Expect = 0.0
 Identities = 286/477 (59%), Positives = 364/477 (76%)
 Frame = +3

Query: 69   VVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEKGAGITC 248
            V+S RV+++G+ V  TL+ GG+L +P   L+I+K++L F  + S+I IK +IE GAGI C
Sbjct: 10   VLSDRVQIDGIVVPLTLTAGGQLRWPDRCLSIEKDVLGFVVEGSRIKIKGVIESGAGICC 69

Query: 249  GVSSQAHVRKTFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRKSATKIFE 428
                 A +RKTF F+ +++++ ++W ++L   + SLGRPKRLFV +NP+GG++ A+KIF 
Sbjct: 70   AGDGGAPLRKTFTFEPLSEDSLRLWNQKLQAVLDSLGRPKRLFVLLNPYGGKRFASKIFL 129

Query: 429  QDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXXXXXXXXX 608
             DVKPLL+DANI  T+QET  +LHAKEV  +LDL  YDG++CVS                
Sbjct: 130  DDVKPLLDDANIHYTLQETKFRLHAKEVAHSLDLLSYDGVLCVSGDGILVEVVNGLLERE 189

Query: 609  DWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVATLRQGET 788
            DW +AI+ PLG++PAGT NGM KSLLDSVGE C+A  ATLAII+GH  SLDVAT+ QG+ 
Sbjct: 190  DWSSAIKMPLGVIPAGTSNGMAKSLLDSVGESCTAFNATLAIIRGHTRSLDVATVSQGQE 249

Query: 789  IYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVPAPGYEPY 968
             +FSVLMLAWGLVADIDIESEKYRWMGS+R DFYA+QRIF LR+Y G I F+ APGYE +
Sbjct: 250  RFFSVLMLAWGLVADIDIESEKYRWMGSARMDFYAIQRIFCLRRYHGCIKFLAAPGYENF 309

Query: 969  GEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFISVWLHNVP 1148
            GEP++L  +         +LN   +  Q + Y GP  D K  N RK+EGPF+S+WLHNVP
Sbjct: 310  GEPLELEGEI-------NELNSNRV--QQNCYCGPAFDMKGFN-RKIEGPFVSIWLHNVP 359

Query: 1149 WGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYIKVKAFIL 1328
            WG ED  AAPDAKFSDG LDL++MKDCS+L+LL+LM+ELN GGHV+SPHV+Y+KVKAF+L
Sbjct: 360  WGGEDVLAAPDAKFSDGYLDLVVMKDCSKLSLLALMSELNKGGHVRSPHVLYLKVKAFVL 419

Query: 1329 EPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATLFTPV 1499
            EPGPRSD+S KEGIIDVDGEVLA G+GTYKS Q+TLMAY+KL I +DQGLAT+F+PV
Sbjct: 420  EPGPRSDDSNKEGIIDVDGEVLARGRGTYKSEQRTLMAYDKLHIEVDQGLATVFSPV 476


>XP_016477647.1 PREDICTED: sphingosine kinase 1-like isoform X1 [Nicotiana tabacum]
          Length = 481

 Score =  577 bits (1488), Expect = 0.0
 Identities = 288/483 (59%), Positives = 366/483 (75%), Gaps = 1/483 (0%)
 Frame = +3

Query: 51   EASSEGVVSSRVKVNGVEVTATLSPGGRLNFPGWSLAIDKEILSFTTQASKIIIKALIEK 230
            EA S  V+S RV++NGV    TL+  G+L +    L I KE+L F  + SKI I+A+ E 
Sbjct: 5    EAGSSPVLSDRVRINGVVTPVTLTAEGKLRWRENCLLIKKEVLGFLIKGSKIRIRAVKEN 64

Query: 231  GAGITCGVSSQAHVRK-TFVFDHVTKEAHQIWTRRLHHFIVSLGRPKRLFVFVNPFGGRK 407
            G GI+CG ++   VR+ +F F+ +T+++ ++W+++L  FI SLGRPKRLFVFVNP+GG+K
Sbjct: 65   GGGISCGGNTGVTVRRRSFTFEPLTQDSLRLWSQKLQEFIDSLGRPKRLFVFVNPYGGKK 124

Query: 408  SATKIFEQDVKPLLEDANIEITVQETAHQLHAKEVVRTLDLSKYDGIVCVSXXXXXXXXX 587
            SA+KIF   VKPLL+DANI+ TVQ T +QLHAKEV ++LD+ +YDG+VCVS         
Sbjct: 125  SASKIFIDSVKPLLDDANIDYTVQATKYQLHAKEVAKSLDILRYDGVVCVSGDGILVEVV 184

Query: 588  XXXXXXXDWETAIRTPLGLVPAGTGNGMVKSLLDSVGEPCSASYATLAIIQGHKTSLDVA 767
                   DW++AI+ PLG++PAGTGNGM KSLLD+VG+ C+AS ATLAII+GHK  LDVA
Sbjct: 185  NGLLEREDWDSAIKMPLGVIPAGTGNGMAKSLLDAVGQSCNASNATLAIIRGHKQLLDVA 244

Query: 768  TLRQGETIYFSVLMLAWGLVADIDIESEKYRWMGSSRFDFYALQRIFSLRKYTGRILFVP 947
            T+ QG+  +FSVLMLAWGL+ADIDIESEKYRWMGS+R ++YA+QRIF LR+Y G I FV 
Sbjct: 245  TVSQGQNRFFSVLMLAWGLIADIDIESEKYRWMGSARINYYAIQRIFRLRRYDGCIKFVA 304

Query: 948  APGYEPYGEPVDLGDQFLGEADSTQKLNEGSLKGQPSHYQGPIVDPKDLNWRKLEGPFIS 1127
            APGYE +GEP D+  + + +  S    ++G        Y GP    KD N RK+EGPF+S
Sbjct: 305  APGYETFGEPTDMEVETIDDVKSNFVQHKG--------YCGPTFHMKDFN-RKIEGPFVS 355

Query: 1128 VWLHNVPWGSEDTKAAPDAKFSDGCLDLILMKDCSRLALLSLMTELNNGGHVKSPHVIYI 1307
            +WLHNVPWG ED  AAPDAKFSDG LDL+++KDC +L LLSLMTELN GGHV+SP+V+Y 
Sbjct: 356  IWLHNVPWGGEDALAAPDAKFSDGYLDLVVIKDCPKLTLLSLMTELNKGGHVRSPYVLYF 415

Query: 1308 KVKAFILEPGPRSDNSAKEGIIDVDGEVLASGKGTYKSNQKTLMAYNKLQITIDQGLATL 1487
            KVKAF+LEPG R D+ +KEGIIDVDGEVLA GKGTYKS+QKTLM YNKL I +DQGLAT+
Sbjct: 416  KVKAFVLEPGSRLDDPSKEGIIDVDGEVLARGKGTYKSDQKTLMTYNKLHIKVDQGLATV 475

Query: 1488 FTP 1496
            F+P
Sbjct: 476  FSP 478