BLASTX nr result
ID: Lithospermum23_contig00008215
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum23_contig00008215
(312 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_011091685.1 PREDICTED: F-box protein PP2-A13 [Sesamum indicum] 166 3e-49
XP_016484921.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 164 1e-48
XP_009758836.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 164 1e-48
XP_016479248.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 164 3e-48
XP_009601679.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 164 3e-48
XP_017216058.1 PREDICTED: F-box protein PP2-A13 [Daucus carota s... 163 4e-48
XP_015900210.1 PREDICTED: F-box protein PP2-A13-like [Ziziphus j... 162 1e-47
XP_019237342.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 162 2e-47
OAY62354.1 hypothetical protein MANES_01G261700 [Manihot esculenta] 162 2e-47
XP_006341734.1 PREDICTED: F-box protein PP2-A13-like [Solanum tu... 162 2e-47
XP_019260009.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 161 4e-47
XP_012083479.1 PREDICTED: F-box protein PP2-A13 [Jatropha curcas... 160 8e-47
XP_016575813.1 PREDICTED: F-box protein PP2-A13-like [Capsicum a... 159 1e-46
XP_009758502.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 159 2e-46
KZV26723.1 hypothetical protein F511_34321 [Dorcoceras hygrometr... 158 3e-46
XP_012843987.1 PREDICTED: F-box protein PP2-A13-like [Erythranth... 159 3e-46
XP_018857829.1 PREDICTED: F-box protein PP2-A13-like [Juglans re... 158 5e-46
XP_019233650.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 158 5e-46
XP_017969786.1 PREDICTED: F-box protein PP2-A13 isoform X1 [Theo... 158 5e-46
XP_016507641.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana ... 157 7e-46
>XP_011091685.1 PREDICTED: F-box protein PP2-A13 [Sesamum indicum]
Length = 291
Score = 166 bits (421), Expect = 3e-49
Identities = 77/103 (74%), Positives = 87/103 (84%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFP G YS+FFRLQLG+V KR GRR NS+N+HGWDIKPV+FQLTT
Sbjct: 153 QIWWLEVDGDLEFQFPRGTYSLFFRLQLGRVGKRLGRRVCNSENVHGWDIKPVQFQLTTL 212
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQHAVS+ LDN+G WV++HVGDF V + TKIKFSLTQI
Sbjct: 213 DGQHAVSRCMLDNLGNWVHFHVGDFTVEYSEELTKIKFSLTQI 255
>XP_016484921.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana tabacum]
Length = 272
Score = 164 bits (416), Expect = 1e-48
Identities = 75/103 (72%), Positives = 88/103 (85%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFPAG YS+FFRL+LGKVTKRH RR N ++IHGWD+KPV+FQLTT
Sbjct: 157 QIWWLEVDGDLEFQFPAGSYSLFFRLKLGKVTKRHNRRVCNYEHIHGWDLKPVQFQLTTS 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG A+S+ L+NVG+WV++HVGDFVV V TKIK+SLTQI
Sbjct: 217 DGDRAISRCYLNNVGSWVHHHVGDFVVKDATVPTKIKYSLTQI 259
>XP_009758836.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana sylvestris]
Length = 287
Score = 164 bits (416), Expect = 1e-48
Identities = 75/103 (72%), Positives = 88/103 (85%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFPAG YS+FFRL+LGKVTKRH RR N ++IHGWD+KPV+FQLTT
Sbjct: 157 QIWWLEVDGDLEFQFPAGSYSLFFRLKLGKVTKRHNRRVCNYEHIHGWDLKPVQFQLTTS 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG A+S+ L+NVG+WV++HVGDFVV V TKIK+SLTQI
Sbjct: 217 DGDRAISRCYLNNVGSWVHHHVGDFVVKDATVPTKIKYSLTQI 259
>XP_016479248.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana tabacum]
Length = 294
Score = 164 bits (414), Expect = 3e-48
Identities = 74/103 (71%), Positives = 87/103 (84%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q+WWLEVDGDLEFQFPAG YS+FFRLQLG+V KR GRR N+D++HGWD+KPV+FQLTT
Sbjct: 157 QMWWLEVDGDLEFQFPAGTYSLFFRLQLGRVAKRLGRRVCNTDHVHGWDLKPVQFQLTTP 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
+GQ A+S+ LDN+GTW N+HVGDFVV V TKIKFSL QI
Sbjct: 217 NGQRAISRCYLDNIGTWTNHHVGDFVVEDPTVVTKIKFSLIQI 259
>XP_009601679.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana tomentosiformis]
Length = 294
Score = 164 bits (414), Expect = 3e-48
Identities = 74/103 (71%), Positives = 87/103 (84%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q+WWLEVDGDLEFQFPAG YS+FFRLQLG+V KR GRR N+D++HGWD+KPV+FQLTT
Sbjct: 157 QMWWLEVDGDLEFQFPAGTYSLFFRLQLGRVAKRLGRRVCNTDHVHGWDLKPVQFQLTTP 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
+GQ A+S+ LDN+GTW N+HVGDFVV V TKIKFSL QI
Sbjct: 217 NGQRAISRCYLDNIGTWTNHHVGDFVVEDPTVVTKIKFSLIQI 259
>XP_017216058.1 PREDICTED: F-box protein PP2-A13 [Daucus carota subsp. sativus]
KZM88090.1 hypothetical protein DCAR_025165 [Daucus
carota subsp. sativus]
Length = 286
Score = 163 bits (413), Expect = 4e-48
Identities = 75/103 (72%), Positives = 83/103 (80%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGD++F+FP G YS+ FRLQLGK+TKR GRR N NIHGWDIKPV+FQLTT
Sbjct: 155 QIWWLEVDGDIDFEFPTGTYSLLFRLQLGKITKRLGRRVCNPGNIHGWDIKPVQFQLTTL 214
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQHAVS+ LDN+G W YH GDFVV K N T IKFSLTQI
Sbjct: 215 DGQHAVSRCFLDNLGNWAYYHAGDFVVEKPNALTNIKFSLTQI 257
>XP_015900210.1 PREDICTED: F-box protein PP2-A13-like [Ziziphus jujuba]
Length = 274
Score = 162 bits (409), Expect = 1e-47
Identities = 74/103 (71%), Positives = 82/103 (79%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q WW EVDG+LEFQFPAG+YS+FFRLQLGK +KR GRR NS+++HGWDIKPV FQLTT
Sbjct: 143 QTWWFEVDGELEFQFPAGKYSLFFRLQLGKSSKRLGRRVCNSEHVHGWDIKPVRFQLTTS 202
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQ AVSQ LDN G W+NYH GDFVV N KIKFS TQI
Sbjct: 203 DGQQAVSQSYLDNPGNWINYHAGDFVVEDPNALMKIKFSATQI 245
>XP_019237342.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana attenuata]
OIT22482.1 f-box protein pp2-a13 [Nicotiana attenuata]
Length = 287
Score = 162 bits (409), Expect = 2e-47
Identities = 73/103 (70%), Positives = 87/103 (84%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFPAG YS+FFRL+LGKVTKRH RR N +++HGWD+KPV+FQLTT
Sbjct: 157 QIWWLEVDGDLEFQFPAGAYSLFFRLKLGKVTKRHNRRVCNYEHVHGWDLKPVQFQLTTA 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG A+S+ L+NVG+WV++HVGDFVV TKIK+SLTQI
Sbjct: 217 DGDRAISRCYLNNVGSWVHHHVGDFVVKDATFPTKIKYSLTQI 259
>OAY62354.1 hypothetical protein MANES_01G261700 [Manihot esculenta]
Length = 290
Score = 162 bits (409), Expect = 2e-47
Identities = 74/102 (72%), Positives = 86/102 (84%)
Frame = -3
Query: 307 IWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQD 128
IWWLEVDG+LEFQFPAG+YS+FFR+QLGK TK+ GRR N+++IHGWDIKPV+F+LTT D
Sbjct: 158 IWWLEVDGELEFQFPAGKYSLFFRVQLGKPTKKMGRRVCNTEHIHGWDIKPVKFELTTSD 217
Query: 127 GQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
GQHA S+ L+N G WVNYHVGDFVV N T IKFSLTQI
Sbjct: 218 GQHAASECFLENPGNWVNYHVGDFVVLHPNELTNIKFSLTQI 259
>XP_006341734.1 PREDICTED: F-box protein PP2-A13-like [Solanum tuberosum]
Length = 295
Score = 162 bits (409), Expect = 2e-47
Identities = 72/103 (69%), Positives = 87/103 (84%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q+WWLEVDGDLEFQFP G YS+FFRLQLG+VTKR GRR NSD++HGWDIKP +FQLTT
Sbjct: 158 QMWWLEVDGDLEFQFPVGTYSLFFRLQLGRVTKRLGRRVCNSDHVHGWDIKPAQFQLTTS 217
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
+ + A+S+ LDNVGTW+++HVGDFVV + TKI+FSLTQI
Sbjct: 218 NDERAISRCYLDNVGTWIHHHVGDFVVEDPTIMTKIRFSLTQI 260
>XP_019260009.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana attenuata]
OIT39477.1 f-box protein pp2-a13 [Nicotiana attenuata]
Length = 293
Score = 161 bits (407), Expect = 4e-47
Identities = 74/103 (71%), Positives = 86/103 (83%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFPAG YS+FFRLQLG+V KR GRR N+D++HGWD+KPV+FQLT
Sbjct: 156 QIWWLEVDGDLEFQFPAGTYSLFFRLQLGRVAKRLGRRVCNTDHVHGWDLKPVQFQLTMP 215
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
+GQ A+S+ LDNVGTW ++HVGDFVV V TKIKFSL QI
Sbjct: 216 NGQRAISRCYLDNVGTWTHHHVGDFVVEDPTVVTKIKFSLIQI 258
>XP_012083479.1 PREDICTED: F-box protein PP2-A13 [Jatropha curcas] KDP28687.1
hypothetical protein JCGZ_14458 [Jatropha curcas]
Length = 294
Score = 160 bits (405), Expect = 8e-47
Identities = 73/103 (70%), Positives = 81/103 (78%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWW E DG+ EFQFPAG YS+FFRLQLGK KR GRR N++ +HGWDIKPV F+L T
Sbjct: 156 QIWWFEADGEFEFQFPAGTYSLFFRLQLGKPLKRMGRRICNTEQVHGWDIKPVRFELMTS 215
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQHAVSQ LDN G W+ YHVGDFVV + N TKIKFSLTQI
Sbjct: 216 DGQHAVSQCFLDNTGNWIQYHVGDFVVERPNELTKIKFSLTQI 258
>XP_016575813.1 PREDICTED: F-box protein PP2-A13-like [Capsicum annuum]
Length = 287
Score = 159 bits (403), Expect = 1e-46
Identities = 72/103 (69%), Positives = 83/103 (80%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEF+FP G YS+FFRL LGK TKRH RRA N +++HGWD+KPV+FQLTT
Sbjct: 157 QIWWLEVDGDLEFKFPEGTYSLFFRLHLGKATKRHSRRACNYEHVHGWDLKPVQFQLTTP 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG S+ LDN+GTW+ +HVGDFVV V TKIKFSLTQI
Sbjct: 217 DGCRVTSRCYLDNIGTWMQHHVGDFVVKDGTVPTKIKFSLTQI 259
>XP_009758502.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana sylvestris]
XP_016510799.1 PREDICTED: F-box protein PP2-A13-like
[Nicotiana tabacum]
Length = 293
Score = 159 bits (403), Expect = 2e-46
Identities = 73/103 (70%), Positives = 86/103 (83%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q+WWLEVDGDLEFQFPAG YS+FFRLQLG+V KR GRR N+D++HGWD+KPV+FQLT
Sbjct: 156 QMWWLEVDGDLEFQFPAGTYSLFFRLQLGRVAKRLGRRICNTDHVHGWDLKPVQFQLTMP 215
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
+GQ A+S+ LDNVGTW ++HVGDFVV V TKIKFSL QI
Sbjct: 216 NGQRAISRCYLDNVGTWTHHHVGDFVVEDPTVVTKIKFSLIQI 258
>KZV26723.1 hypothetical protein F511_34321 [Dorcoceras hygrometricum]
Length = 277
Score = 158 bits (400), Expect = 3e-46
Identities = 71/103 (68%), Positives = 83/103 (80%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
Q+WWL+VDGDLEF+ PAG YSMFFRLQLG++ KR GRR NSDN+HGW KP FQLTT
Sbjct: 156 QMWWLQVDGDLEFKLPAGTYSMFFRLQLGRICKRLGRRVCNSDNVHGWKTKPAHFQLTTH 215
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQHA S+ LDN+G W++YH+GDFVV + TKIKFSLTQI
Sbjct: 216 DGQHAESRCILDNIGNWLHYHIGDFVVKDADSFTKIKFSLTQI 258
>XP_012843987.1 PREDICTED: F-box protein PP2-A13-like [Erythranthe guttata]
XP_012845872.1 PREDICTED: F-box protein PP2-A13-like
[Erythranthe guttata] EYU30316.1 hypothetical protein
MIMGU_mgv1a011147mg [Erythranthe guttata] EYU32063.1
hypothetical protein MIMGU_mgv1a011143mg [Erythranthe
guttata]
Length = 291
Score = 159 bits (401), Expect = 3e-46
Identities = 71/103 (68%), Positives = 84/103 (81%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDG+LEF+FP G YSMFFRL LG+V K+ RR N DN+HGWD+KP +FQLTT+
Sbjct: 153 QIWWLEVDGNLEFKFPKGTYSMFFRLHLGRVGKKSNRRIRNLDNVHGWDLKPAQFQLTTE 212
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG +VS+ LDN+G W+NYHVGDFVV +N TKIKFSLTQI
Sbjct: 213 DGHQSVSRCMLDNLGHWINYHVGDFVVEDSNALTKIKFSLTQI 255
>XP_018857829.1 PREDICTED: F-box protein PP2-A13-like [Juglans regia]
Length = 284
Score = 158 bits (399), Expect = 5e-46
Identities = 74/103 (71%), Positives = 80/103 (77%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDG+ EFQFP G YS+FFRLQLGK +KR GRR +S++IHGWDIKPV FQLTT
Sbjct: 153 QIWWLEVDGEFEFQFPPGTYSLFFRLQLGKSSKRMGRRVCDSEHIHGWDIKPVRFQLTTS 212
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQHA SQ LDN G WV YH GDFVV N KI FSLTQI
Sbjct: 213 DGQHAASQCHLDNHGNWVYYHAGDFVVRNTNALMKINFSLTQI 255
>XP_019233650.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana attenuata]
OIT27241.1 f-box protein pp2-a13 [Nicotiana attenuata]
Length = 288
Score = 158 bits (399), Expect = 5e-46
Identities = 74/104 (71%), Positives = 89/104 (85%), Gaps = 1/104 (0%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRA-SNSDNIHGWDIKPVEFQLTT 134
QIWW+EVDG+LEFQFPAG YS+FFRLQLGKVTKR GRR +N++ +HGWD+KPV+FQLTT
Sbjct: 157 QIWWVEVDGELEFQFPAGTYSVFFRLQLGKVTKRLGRRVITNTEQVHGWDLKPVQFQLTT 216
Query: 133 QDGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQ A+S+ LDNVGTWV++HVGDFVV + TK+KFSL QI
Sbjct: 217 SDGQCAMSKCYLDNVGTWVHHHVGDFVVKDSAAMTKLKFSLMQI 260
>XP_017969786.1 PREDICTED: F-box protein PP2-A13 isoform X1 [Theobroma cacao]
Length = 288
Score = 158 bits (399), Expect = 5e-46
Identities = 73/103 (70%), Positives = 82/103 (79%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWW EVDG+LEFQFPAG Y +FFRLQLGK +KR GRR N++++HGWDIKP FQLTT
Sbjct: 156 QIWWFEVDGELEFQFPAGTYCLFFRLQLGKSSKRLGRRFCNAEHVHGWDIKPARFQLTTS 215
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DGQ AVSQ LDN G WV YHVGDF+V N TKIKFS+TQI
Sbjct: 216 DGQRAVSQCYLDNPGNWVLYHVGDFIVENPNALTKIKFSMTQI 258
>XP_016507641.1 PREDICTED: F-box protein PP2-A13-like [Nicotiana tabacum]
Length = 287
Score = 157 bits (398), Expect = 7e-46
Identities = 73/103 (70%), Positives = 85/103 (82%)
Frame = -3
Query: 310 QIWWLEVDGDLEFQFPAGRYSMFFRLQLGKVTKRHGRRASNSDNIHGWDIKPVEFQLTTQ 131
QIWWLEVDGDLEFQFPAG YS+FFRL+LGKVTKRH RR N ++IHGWD+KPV+FQL T
Sbjct: 157 QIWWLEVDGDLEFQFPAGIYSLFFRLKLGKVTKRHNRRVCNYEHIHGWDLKPVQFQLATP 216
Query: 130 DGQHAVSQKTLDNVGTWVNYHVGDFVVGKNNVSTKIKFSLTQI 2
DG A S+ L+NVG+WV++H GDFVV V TKIK+SLTQI
Sbjct: 217 DGDRARSRCYLNNVGSWVHHHAGDFVVKDATVPTKIKYSLTQI 259