BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00005764
         (2154 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_011099760.1 PREDICTED: putative tRNA pseudouridine synthase P...   592   0.0  
XP_015161917.1 PREDICTED: putative tRNA pseudouridine synthase P...   585   0.0  
XP_004238405.1 PREDICTED: putative tRNA pseudouridine synthase P...   583   0.0  
XP_015074305.1 PREDICTED: putative tRNA pseudouridine synthase P...   583   0.0  
XP_006342101.1 PREDICTED: putative tRNA pseudouridine synthase P...   580   0.0  
XP_010320448.1 PREDICTED: putative tRNA pseudouridine synthase P...   578   0.0  
XP_015074304.1 PREDICTED: putative tRNA pseudouridine synthase P...   577   0.0  
XP_009595594.1 PREDICTED: putative tRNA pseudouridine synthase P...   573   0.0  
XP_012839211.1 PREDICTED: putative tRNA pseudouridine synthase P...   572   0.0  
XP_009775535.1 PREDICTED: putative tRNA pseudouridine synthase P...   572   0.0  
XP_019260000.1 PREDICTED: putative tRNA pseudouridine synthase P...   572   0.0  
XP_009595593.1 PREDICTED: putative tRNA pseudouridine synthase P...   570   0.0  
XP_016432383.1 PREDICTED: putative tRNA pseudouridine synthase P...   569   0.0  
XP_019186230.1 PREDICTED: putative tRNA pseudouridine synthase P...   566   0.0  
XP_009775534.1 PREDICTED: putative tRNA pseudouridine synthase P...   566   0.0  
XP_019259999.1 PREDICTED: putative tRNA pseudouridine synthase P...   566   0.0  
XP_016432382.1 PREDICTED: putative tRNA pseudouridine synthase P...   566   0.0  
CBI18893.3 unnamed protein product, partial [Vitis vinifera]          566   0.0  
EOY15171.1 Pseudouridine synthase family protein, putative isofo...   563   0.0  
XP_011041278.1 PREDICTED: putative tRNA pseudouridine synthase P...   561   0.0  

>XP_011099760.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 [Sesamum
            indicum]
          Length = 558

 Score =  592 bits (1527), Expect = 0.0
 Identities = 305/532 (57%), Positives = 383/532 (71%), Gaps = 32/532 (6%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLP-QGDYAD 299
            +V ++P   +KDL+S GVC RCI  LF  ++ ICS PS+ T  L+    +V   +GD   
Sbjct: 34   SVRAVPAQPLKDLLSNGVCVRCIFRLFSLHEQICSRPSLVTPILSTILREVTGCEGDGTS 93

Query: 300  ----------------------QVCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-- 407
                                  +VCSVCLGILQF Y D+NG  VK +SAS+ AA +    
Sbjct: 94   SSPRETKDLNDLESLREVKEEIKVCSVCLGILQFLYNDDNGTLVKKDSASDFAAAMEEVV 153

Query: 408  ---DHQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSKS----TKDVFKMAI 566
                HQ D+F+L+VS+P  V++N++AVWLYI+ K+SS+  FLE   S    TKD  K+++
Sbjct: 154  KQEGHQSDSFSLEVSLPQDVVENEQAVWLYIQEKYSSESWFLEKLPSECFATKDFLKLSV 213

Query: 567  TNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDTSDTNGFSKRRKTGNVEGIRTVQSCT 746
            TN LET LG+K   +SF++RLTYTL+  S   +   + NGF KRRKT + +  +      
Sbjct: 214  TNPLETLLGIKCNVSSFRVRLTYTLSDASTRDRHIKEKNGFCKRRKTDDSDNFKK----- 268

Query: 747  AAGEASADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYL 926
               +     ++ +  +   S  L+   +   K PLEKVNQ C L + CYR  +++GGRYL
Sbjct: 269  ---DGGFHRVEGHDLTRKSSTALEDGRVGLSKFPLEKVNQCCSLNFQCYRTSVYVGGRYL 325

Query: 927  KFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRP 1106
            K+SRNVSQ+RWIIDDERMGEAS+EEI+G  +LPM +GDTYKFHAAGREDIDVRMLGTGRP
Sbjct: 326  KYSRNVSQTRWIIDDERMGEASIEEIVGGNILPMCQGDTYKFHAAGREDIDVRMLGTGRP 385

Query: 1107 FLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYA 1286
            FL+EIQNARQVPS   +K +E +IN+LESK +KVK+LK LG+ GW+LMREGEAEKQKQYA
Sbjct: 386  FLVEIQNARQVPSDALVKDIENKINSLESKFVKVKNLKVLGNQGWELMREGEAEKQKQYA 445

Query: 1287 ALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQY 1466
            ALVWI RP+ND+D+  + S++DM+I+QKTP+RVLHRRSPL REK IHWMR+E+IAGS+QY
Sbjct: 446  ALVWIPRPLNDDDVQTLASVRDMQILQKTPIRVLHRRSPLEREKTIHWMRIERIAGSSQY 505

Query: 1467 FLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            FLLHLCTQAGTYIKEFVHGDLGRTHPSIGSIL CRAEILQLDVTDVKMDCFQ
Sbjct: 506  FLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILKCRAEILQLDVTDVKMDCFQ 557


>XP_015161917.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum tuberosum]
          Length = 557

 Score =  585 bits (1508), Expect = 0.0
 Identities = 304/532 (57%), Positives = 384/532 (72%), Gaps = 29/532 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV +LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +  G++
Sbjct: 33   EAVHALPVNAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRKKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P  V +N +AVWLY+K K+  +L F E ++    STKD+ K+ + N L+  LGV
Sbjct: 153  SLEVSLPSSVTENDQAVWLYMKNKFRHELWFQEKAECQFISTKDILKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKTGNVEGIRTV-QSCTAAGEAS-- 764
            KS+++SF++RLTY+  H+ A T+D   D NG +KRRKTG    ++T  +S T   E+S  
Sbjct: 213  KSSQSSFRVRLTYS--HSDALTRDDVQDGNGGNKRRKTGFTGDLKTTGESVTPHVESSDN 270

Query: 765  ----ADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKF 932
                 DGL K    E+     Q    NN    L+KV +PCCLT  C R+  +IGGRYLK+
Sbjct: 271  HIVDGDGLTKVCPKES-----QDLSCNNLMAQLDKVGKPCCLTIHCCRSSTYIGGRYLKY 325

Query: 933  SRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFL 1112
            SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGRPFL
Sbjct: 326  SRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGRPFL 385

Query: 1113 IEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAAL 1292
            +EIQNARQVPS   +K++E +IN+LES  ++VK+L+ +G  GWDLMREGEAEKQKQYAAL
Sbjct: 386  VEIQNARQVPSEALIKEIERKINSLESHYVRVKNLRAVGIQGWDLMREGEAEKQKQYAAL 445

Query: 1293 VWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFL 1472
            VWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+ E+++GS+QYFL
Sbjct: 446  VWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQAERVSGSSQYFL 505

Query: 1473 LHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            LHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  LHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 557


>XP_004238405.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum lycopersicum]
          Length = 557

 Score =  583 bits (1503), Expect = 0.0
 Identities = 303/532 (56%), Positives = 384/532 (72%), Gaps = 29/532 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVL--PQGDY 293
            +AV +LP++AVKDL S+G C RCI  LFG ++ +CS PS+  S        V+    G++
Sbjct: 33   EAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRNKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P +V +N +AVWLY+K K+  +L F E ++    STKD+FK+ + N L+  LGV
Sbjct: 153  SLEVSLPSVVTENDQAVWLYMKNKYRHELWFQEKAECQFISTKDIFKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKTGNVEGIRTV-QSCTAAGEA--- 761
            KST++SF++RLTY+  H+ A T+D   + NG +KRRKTG    ++T  +S T   E+   
Sbjct: 213  KSTQSSFRVRLTYS--HSDALTRDDVQEGNGGNKRRKTGFTGDLKTTGESVTPHVESFDS 270

Query: 762  ---SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKF 932
                 DGL K    EA     Q    NN    L+KV +PC L   C R+  +IGGRYLK+
Sbjct: 271  NIVDGDGLTKVCPKEA-----QDLSCNNLMAKLDKVGKPCSLAIHCCRSSTYIGGRYLKY 325

Query: 933  SRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFL 1112
            SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGRPFL
Sbjct: 326  SRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGRPFL 385

Query: 1113 IEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAAL 1292
            +EIQNARQVPS   +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGEAEKQKQYAAL
Sbjct: 386  VEIQNARQVPSEALIKEIEKKINSLESQYVRVKNLRAVGSQGWDLMREGEAEKQKQYAAL 445

Query: 1293 VWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFL 1472
            VWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE+++GS+QY L
Sbjct: 446  VWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQVERVSGSSQYLL 505

Query: 1473 LHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            LHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  LHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 557


>XP_015074305.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Solanum pennellii]
          Length = 557

 Score =  583 bits (1502), Expect = 0.0
 Identities = 302/532 (56%), Positives = 385/532 (72%), Gaps = 29/532 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV +LP++AVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +  G++
Sbjct: 33   EAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRKKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P +V +N +AVWLY+K K+  +L F E ++    STKD+FK+ + N L+  LGV
Sbjct: 153  SLEVSLPSVVTENDQAVWLYMKNKYRHELWFQEKAECQFISTKDIFKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKTGNVEGIRTV-QSCTAAGEA--- 761
            KST++SF++RLTY+  H+ A T+D   + NG +KRRKTG    ++T  +S T   E+   
Sbjct: 213  KSTQSSFRVRLTYS--HSDALTRDDVQEGNGGNKRRKTGFTGDLKTTGESVTPHVESFDS 270

Query: 762  ---SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKF 932
                 DGL K    EA     Q    NN    L+K+ +PC L   C R+  +IGGRYLK+
Sbjct: 271  NIVDGDGLTKVCPKEA-----QDLSCNNLMAKLDKIGKPCSLAIHCCRSSTYIGGRYLKY 325

Query: 933  SRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFL 1112
            SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGRPFL
Sbjct: 326  SRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGRPFL 385

Query: 1113 IEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAAL 1292
            +EIQNARQVPS   +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGEAEKQKQYAAL
Sbjct: 386  VEIQNARQVPSEALIKEIEKKINSLESQYVRVKNLRAVGSQGWDLMREGEAEKQKQYAAL 445

Query: 1293 VWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFL 1472
            VWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE+++GS+QY L
Sbjct: 446  VWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQVERVSGSSQYLL 505

Query: 1473 LHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            LHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  LHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 557


>XP_006342101.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum tuberosum]
          Length = 560

 Score =  580 bits (1494), Expect = 0.0
 Identities = 304/535 (56%), Positives = 384/535 (71%), Gaps = 32/535 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV +LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +  G++
Sbjct: 33   EAVHALPVNAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRKKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P  V +N +AVWLY+K K+  +L F E ++    STKD+ K+ + N L+  LGV
Sbjct: 153  SLEVSLPSSVTENDQAVWLYMKNKFRHELWFQEKAECQFISTKDILKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKT---GNVEGIRTV-QSCTAAGEA 761
            KS+++SF++RLTY+  H+ A T+D   D NG +KRRKT   G    ++T  +S T   E+
Sbjct: 213  KSSQSSFRVRLTYS--HSDALTRDDVQDGNGGNKRRKTDKAGFTGDLKTTGESVTPHVES 270

Query: 762  S------ADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRY 923
            S       DGL K    E+     Q    NN    L+KV +PCCLT  C R+  +IGGRY
Sbjct: 271  SDNHIVDGDGLTKVCPKES-----QDLSCNNLMAQLDKVGKPCCLTIHCCRSSTYIGGRY 325

Query: 924  LKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGR 1103
            LK+SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGR
Sbjct: 326  LKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGR 385

Query: 1104 PFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQY 1283
            PFL+EIQNARQVPS   +K++E +IN+LES  ++VK+L+ +G  GWDLMREGEAEKQKQY
Sbjct: 386  PFLVEIQNARQVPSEALIKEIERKINSLESHYVRVKNLRAVGIQGWDLMREGEAEKQKQY 445

Query: 1284 AALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQ 1463
            AALVWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+ E+++GS+Q
Sbjct: 446  AALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQAERVSGSSQ 505

Query: 1464 YFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            YFLLHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  YFLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 560


>XP_010320448.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum lycopersicum]
          Length = 560

 Score =  578 bits (1489), Expect = 0.0
 Identities = 303/535 (56%), Positives = 384/535 (71%), Gaps = 32/535 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVL--PQGDY 293
            +AV +LP++AVKDL S+G C RCI  LFG ++ +CS PS+  S        V+    G++
Sbjct: 33   EAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRNKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P +V +N +AVWLY+K K+  +L F E ++    STKD+FK+ + N L+  LGV
Sbjct: 153  SLEVSLPSVVTENDQAVWLYMKNKYRHELWFQEKAECQFISTKDIFKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKT---GNVEGIRTV-QSCTAAGEA 761
            KST++SF++RLTY+  H+ A T+D   + NG +KRRKT   G    ++T  +S T   E+
Sbjct: 213  KSTQSSFRVRLTYS--HSDALTRDDVQEGNGGNKRRKTDKAGFTGDLKTTGESVTPHVES 270

Query: 762  ------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRY 923
                    DGL K    EA     Q    NN    L+KV +PC L   C R+  +IGGRY
Sbjct: 271  FDSNIVDGDGLTKVCPKEA-----QDLSCNNLMAKLDKVGKPCSLAIHCCRSSTYIGGRY 325

Query: 924  LKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGR 1103
            LK+SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGR
Sbjct: 326  LKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGR 385

Query: 1104 PFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQY 1283
            PFL+EIQNARQVPS   +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGEAEKQKQY
Sbjct: 386  PFLVEIQNARQVPSEALIKEIEKKINSLESQYVRVKNLRAVGSQGWDLMREGEAEKQKQY 445

Query: 1284 AALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQ 1463
            AALVWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE+++GS+Q
Sbjct: 446  AALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQVERVSGSSQ 505

Query: 1464 YFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            Y LLHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  YLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 560


>XP_015074304.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Solanum pennellii]
          Length = 560

 Score =  577 bits (1488), Expect = 0.0
 Identities = 302/535 (56%), Positives = 385/535 (71%), Gaps = 32/535 (5%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV +LP++AVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +  G++
Sbjct: 33   EAVHALPVSAVKDLWSIGACVRCIFRLFGLHELMCSHPSLSISKWCDILEDVMRKKDGEF 92

Query: 294  ADQ----------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTF 428
             +Q           C +CLGILQF Y D+ G  +K  SA + A  I         Q+D+F
Sbjct: 93   NEQSHQERSRETNFCKICLGILQFLYYDDTGTLLKKYSADDFAVVISELVKQQYQQIDSF 152

Query: 429  ALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAITNTLETSLGV 596
            +L+VS+P +V +N +AVWLY+K K+  +L F E ++    STKD+FK+ + N L+  LGV
Sbjct: 153  SLEVSLPSVVTENDQAVWLYMKNKYRHELWFQEKAECQFISTKDIFKLLVINPLQRLLGV 212

Query: 597  KSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKT---GNVEGIRTV-QSCTAAGEA 761
            KST++SF++RLTY+  H+ A T+D   + NG +KRRKT   G    ++T  +S T   E+
Sbjct: 213  KSTQSSFRVRLTYS--HSDALTRDDVQEGNGGNKRRKTDKAGFTGDLKTTGESVTPHVES 270

Query: 762  ------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRY 923
                    DGL K    EA     Q    NN    L+K+ +PC L   C R+  +IGGRY
Sbjct: 271  FDSNIVDGDGLTKVCPKEA-----QDLSCNNLMAKLDKIGKPCSLAIHCCRSSTYIGGRY 325

Query: 924  LKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGR 1103
            LK+SRNVSQ+RW+IDDERMGEASVEEIIG  +LP+ +GD YKFHAAGREDIDVRMLGTGR
Sbjct: 326  LKYSRNVSQTRWVIDDERMGEASVEEIIGGAILPIFQGDNYKFHAAGREDIDVRMLGTGR 385

Query: 1104 PFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQY 1283
            PFL+EIQNARQVPS   +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGEAEKQKQY
Sbjct: 386  PFLVEIQNARQVPSEALIKEIEKKINSLESQYVRVKNLRAVGSQGWDLMREGEAEKQKQY 445

Query: 1284 AALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQ 1463
            AALVWISRP++D DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE+++GS+Q
Sbjct: 446  AALVWISRPVSDEDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMQVERVSGSSQ 505

Query: 1464 YFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            Y LLHLCTQAGTYIKEFVHGD GRT PSIGSILGCR EILQLDVTDVKMDCFQ+E
Sbjct: 506  YLLLHLCTQAGTYIKEFVHGDFGRTQPSIGSILGCRTEILQLDVTDVKMDCFQEE 560


>XP_009595594.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana tomentosiformis]
          Length = 564

 Score =  573 bits (1477), Expect = 0.0
 Identities = 297/542 (54%), Positives = 380/542 (70%), Gaps = 40/542 (7%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVL-------- 278
            AV  LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+        
Sbjct: 30   AVNELPVNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADVMRKEDVDTA 89

Query: 279  --PQGDYADQV-------------CSVCLGILQFAYMDNNGVFVKNESASELAATIITD- 410
              PQ D    +             C +CLGILQF Y D++G  +K +SA + A  +    
Sbjct: 90   DHPQKDGEFSIKQLHQEPLKETNFCKICLGILQFLYNDDSGTLLKKDSADDFALAVSEPV 149

Query: 411  ----HQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAI 566
                HQ+D+F+L+ S+P  + +N +AVWLY+K ++  +L F E ++     TKD+ K+ +
Sbjct: 150  KQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYHHELWFQEKAEYQFICTKDILKLLV 209

Query: 567  TNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDT-SDTNGFSKRRKTGNVEGIRTVQSC 743
            TN L++ LGVKS++ S +IRLTY+  H+ A T+D   + NG +KRRKTG    ++T +  
Sbjct: 210  TNPLQSLLGVKSSQGSLRIRLTYS--HSDALTRDDIQERNGGNKRRKTGFTGDLKTTEES 267

Query: 744  TAAGEAS-------ADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNP 902
                  S        DGL K    ++     Q    +     L KV++PCCLT  C R+P
Sbjct: 268  VTPHVESFDNHIGDGDGLTKVCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHCCRSP 322

Query: 903  IFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDV 1082
            IFIGGRYLK+SRNVSQ+ W+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGREDIDV
Sbjct: 323  IFIGGRYLKYSRNVSQTPWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAAGREDIDV 382

Query: 1083 RMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGE 1262
            RMLGTGRPFL+EIQNAR+VPS V +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGE
Sbjct: 383  RMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWDLMREGE 442

Query: 1263 AEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVE 1442
            AEKQKQY ALVWISRP++D+DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE
Sbjct: 443  AEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMKVE 502

Query: 1443 KIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            +I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCRAEILQLDVTDVKMDCFQ
Sbjct: 503  RISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRAEILQLDVTDVKMDCFQ 562

Query: 1623 DE 1628
             E
Sbjct: 563  AE 564


>XP_012839211.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 [Erythranthe
            guttata]
          Length = 556

 Score =  572 bits (1474), Expect = 0.0
 Identities = 301/530 (56%), Positives = 371/530 (70%), Gaps = 30/530 (5%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQGDYADQ 302
            AV SLP   VKDL+  GVC RCI  LF  +  ICS  S+ T  L+   +++   G+ A+ 
Sbjct: 34   AVRSLPPQPVKDLLLNGVCTRCIFRLFCLHKQICSPSSMATRNLSSVLHELREHGNGANN 93

Query: 303  ---------------------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT---- 407
                                 VC VCLGILQF Y D  GV V+  +A + AATI      
Sbjct: 94   GVRETNDLTDTGYVQEFEEEFVCRVCLGILQFVYSDGKGVLVEKNNAGDFAATIEDAVKK 153

Query: 408  -DHQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSKS----TKDVFKMAITN 572
              H++D F L+VS+PP+V++ ++AVW YI+ K+SS+  F E   S     KD  K+++ N
Sbjct: 154  EGHEIDNFCLEVSLPPIVVEKEQAVWSYIEKKYSSESWFSEKFPSERIGVKDFLKLSVAN 213

Query: 573  TLETSLGVKSTETSFQIRLTYTLAHTSASTQDTSDTNGFSKRRKTGNVEGIRTVQSCTAA 752
             LE  L VKS  +SF+I LTYTL+  S+  +   ++NG SKRRK   ++    +++CT  
Sbjct: 214  PLEKLLDVKSNGSSFRICLTYTLSDASSKIEPLPESNGCSKRRK---IDDSDVLKNCTGL 270

Query: 753  GEASADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKF 932
             E            E+V      +G      PLEKVN  C L   CY+  I++GGRYLK+
Sbjct: 271  HEVEKHNTVTNSSIESVD-----EGNGFLPFPLEKVNNCCSLISQCYKTSIYVGGRYLKY 325

Query: 933  SRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFL 1112
            SRNVSQSRWIIDDERMGEASVEEIIG  +LP+ +GD+YKFHAAGREDIDVRMLGTGRPFL
Sbjct: 326  SRNVSQSRWIIDDERMGEASVEEIIGGSILPICQGDSYKFHAAGREDIDVRMLGTGRPFL 385

Query: 1113 IEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAAL 1292
            +EIQNARQ PS V +K +EM+IN+LE   +KVK+LK LGS GW +MREGEAEKQKQYAAL
Sbjct: 386  VEIQNARQFPSDVLIKDMEMKINSLERNLVKVKNLKFLGSQGWVMMREGEAEKQKQYAAL 445

Query: 1293 VWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFL 1472
            VWISRP+ND+D+ ++ S KDM+I+QKTPVRVLHRRSPLGREK IHWM+VE++AGS+QYF+
Sbjct: 446  VWISRPLNDDDVLSLSSFKDMQILQKTPVRVLHRRSPLGREKTIHWMKVERVAGSSQYFM 505

Query: 1473 LHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            LHLCTQAGTYIKEFVHGDLGRT PS+GSILGCRAEILQLDVTDVKMDCFQ
Sbjct: 506  LHLCTQAGTYIKEFVHGDLGRTQPSVGSILGCRAEILQLDVTDVKMDCFQ 555


>XP_009775535.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana sylvestris]
          Length = 564

 Score =  572 bits (1474), Expect = 0.0
 Identities = 298/543 (54%), Positives = 383/543 (70%), Gaps = 40/543 (7%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV  LP+NAVKDL S+G C RC+  L G  + +CS PS+  S        V+ +  GD 
Sbjct: 29   EAVNELPVNAVKDLWSIGACVRCVFRLLGLRELMCSHPSLSISKWCDILEDVMRKEDGDT 88

Query: 294  ADQ---------------------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT- 407
            ADQ                      C +CLGILQF Y D+NG  +K +SA++ A  I   
Sbjct: 89   ADQPQKDGELSNKQLHQEPLKETNFCKICLGILQFLYNDDNGTLLKKDSANDFALVIAEL 148

Query: 408  ----DHQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMA 563
                 HQ+D+F+L+VS+P  + +  +AV LY+K ++  +L F E ++    STKD+ K+ 
Sbjct: 149  LKQQHHQIDSFSLEVSVPSALAETDQAVRLYMKNRYHHELWFQEKAECQFISTKDILKLL 208

Query: 564  ITNTLETSLGVKSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKTGNVEGIRTV-Q 737
            + N L+  LGVKS+++SF++RLTY+  H+ A T+D   +  G +KRRKTG    ++T  +
Sbjct: 209  VINPLQKLLGVKSSQSSFRVRLTYS--HSDALTRDDVQERAGGNKRRKTGFTGDLKTTKE 266

Query: 738  SCTAAGEA------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRN 899
            S T   E+        DGL K    ++     Q    +     L KV++PCCLT  C R+
Sbjct: 267  SVTPHVESFDNHIGDGDGLMKLCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHCCRS 321

Query: 900  PIFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDID 1079
            PIFIGGRYLK+SRNVSQ+RW+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGREDID
Sbjct: 322  PIFIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAAGREDID 381

Query: 1080 VRMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREG 1259
            VRMLGTGRPFL+EIQNARQVPS V +K++E +IN++ES+ ++VK+L+ +GS GWDLMREG
Sbjct: 382  VRMLGTGRPFLVEIQNARQVPSEVLIKEIERKINSVESRLVRVKNLRAVGSQGWDLMREG 441

Query: 1260 EAEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRV 1439
            EAEKQKQY ALVWISRP++D DL  I SLK++K+ Q+TP+RVLHRRSPL REKIIHWM+V
Sbjct: 442  EAEKQKQYTALVWISRPVSDEDLQTISSLKELKVAQRTPIRVLHRRSPLEREKIIHWMKV 501

Query: 1440 EKIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCF 1619
            E+I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCR EILQLDVTDVKMDCF
Sbjct: 502  ERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRTEILQLDVTDVKMDCF 561

Query: 1620 QDE 1628
            Q E
Sbjct: 562  QAE 564


>XP_019260000.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana attenuata] OIT39489.1 hypothetical protein
            A4A49_14211 [Nicotiana attenuata]
          Length = 564

 Score =  572 bits (1473), Expect = 0.0
 Identities = 302/567 (53%), Positives = 394/567 (69%), Gaps = 41/567 (7%)
 Frame = +3

Query: 45   GRNSLELGSPSPYEKMGGRQEADVLK-AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCI 221
            G  + E+ S     ++   Q+  +L+ AV  LP+NAVKDL S+G C RC+  LFG ++ +
Sbjct: 3    GEVAAEVNSVEGNMQLSEEQKLRLLREAVNELPVNAVKDLWSIGACVRCVFRLFGLHELM 62

Query: 222  CSSPSIPTSALARAFNQVLPQGDY--ADQ---------------------VCSVCLGILQ 332
            CS PS+  S        V+ + D   ADQ                      C++CLGILQ
Sbjct: 63   CSHPSLSISKWCDILEDVMRKEDVDTADQPQKDGELSNKQLHQEPLKETNFCTICLGILQ 122

Query: 333  FAYMDNNGVFVKNESASELAATIIT-----DHQLDTFALQVSIPPLVLDNQKAVWLYIKT 497
            F Y D+NG  +K +SA++ A  I        HQ+D+F+L+VS+P  + +  +AV LY+K 
Sbjct: 123  FLYNDDNGTLLKKDSANDFALVIAELLKQQHHQIDSFSLEVSVPSALAETDQAVRLYMKN 182

Query: 498  KWSSQLEFLELSK----STKDVFKMAITNTLETSLGVKSTETSFQIRLTYTLAHTSASTQ 665
            ++  +L F E ++    STKD+ K  + N L+  LGVKS+++SF++RLTY+  H+ A T+
Sbjct: 183  RYHHELWFQEKAECQFISTKDILKSLVINPLQRLLGVKSSQSSFRVRLTYS--HSDALTR 240

Query: 666  D-TSDTNGFSKRRKTGNVEGIRTV-QSCTAAGEA------SADGLKKYIDSEAVSNELQV 821
            D   +  G +KRRKTG    ++T  +S T   E+        DGL K    ++     Q 
Sbjct: 241  DGVQERAGGNKRRKTGFSGDLKTTKESVTPHVESFDNHIGDGDGLTKVCPKDS-----QD 295

Query: 822  QGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKFSRNVSQSRWIIDDERMGEASVEE 1001
               N     L KV++PCCLT  C R+PIFIGGRYLK+SRNVSQ+RW+IDDERMGEASVEE
Sbjct: 296  LSCNYLMAQLNKVSKPCCLTIHCCRSPIFIGGRYLKYSRNVSQTRWVIDDERMGEASVEE 355

Query: 1002 IIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFLIEIQNARQVPSSVFMKQVEMRIN 1181
            I+G  +LP+ +G+ YKFHAAGREDIDVRMLGTGRPFL+EIQNARQVPS V +K++E +IN
Sbjct: 356  IVGGAILPIFQGNNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEVLIKEIERKIN 415

Query: 1182 NLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAALVWISRPINDNDLHAIVSLKDMKI 1361
            ++ES+ ++VK+L+ +GS GWDLMREGEAEKQKQY ALVWISRP++D DL  I SLK++K+
Sbjct: 416  SVESRLVRVKNLRAVGSQGWDLMREGEAEKQKQYTALVWISRPVSDEDLQTISSLKELKV 475

Query: 1362 MQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTH 1541
             Q+TP+RVLHRRSPL REKIIHWM+VE+I+GS+QY LLHLCTQAGTYIKEFVHGD GRT 
Sbjct: 476  AQRTPIRVLHRRSPLEREKIIHWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQ 535

Query: 1542 PSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            PS+GSILGCR EILQLDVTDVKMDCFQ
Sbjct: 536  PSVGSILGCRTEILQLDVTDVKMDCFQ 562


>XP_009595593.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tomentosiformis] XP_018624870.1 PREDICTED:
            putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tomentosiformis]
          Length = 567

 Score =  570 bits (1468), Expect = 0.0
 Identities = 299/545 (54%), Positives = 382/545 (70%), Gaps = 43/545 (7%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVL-------- 278
            AV  LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+        
Sbjct: 30   AVNELPVNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADVMRKEDVDTA 89

Query: 279  --PQGDYADQV-------------CSVCLGILQFAYMDNNGVFVKNESASELAATIITD- 410
              PQ D    +             C +CLGILQF Y D++G  +K +SA + A  +    
Sbjct: 90   DHPQKDGEFSIKQLHQEPLKETNFCKICLGILQFLYNDDSGTLLKKDSADDFALAVSEPV 149

Query: 411  ----HQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAI 566
                HQ+D+F+L+ S+P  + +N +AVWLY+K ++  +L F E ++     TKD+ K+ +
Sbjct: 150  KQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYHHELWFQEKAEYQFICTKDILKLLV 209

Query: 567  TNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDT-SDTNGFSKRRKTGNV----EGIRT 731
            TN L++ LGVKS++ S +IRLTY+  H+ A T+D   + NG +KRRKTG      +   T
Sbjct: 210  TNPLQSLLGVKSSQGSLRIRLTYS--HSDALTRDDIQERNGGNKRRKTGKAGFTGDLKTT 267

Query: 732  VQSCTAAGEA------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCY 893
             +S T   E+        DGL K    ++     Q    +     L KV++PCCLT  C 
Sbjct: 268  EESVTPHVESFDNHIGDGDGLTKVCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHCC 322

Query: 894  RNPIFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGRED 1073
            R+PIFIGGRYLK+SRNVSQ+ W+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGRED
Sbjct: 323  RSPIFIGGRYLKYSRNVSQTPWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAAGRED 382

Query: 1074 IDVRMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMR 1253
            IDVRMLGTGRPFL+EIQNAR+VPS V +K++E +IN+LES+ ++VK+L+ +GS GWDLMR
Sbjct: 383  IDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWDLMR 442

Query: 1254 EGEAEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWM 1433
            EGEAEKQKQY ALVWISRP++D+DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM
Sbjct: 443  EGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWM 502

Query: 1434 RVEKIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMD 1613
            +VE+I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCRAEILQLDVTDVKMD
Sbjct: 503  KVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRAEILQLDVTDVKMD 562

Query: 1614 CFQDE 1628
            CFQ E
Sbjct: 563  CFQAE 567


>XP_016432383.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Nicotiana tabacum]
          Length = 564

 Score =  569 bits (1467), Expect = 0.0
 Identities = 296/542 (54%), Positives = 379/542 (69%), Gaps = 40/542 (7%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDYA 296
            AV  LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +   D A
Sbjct: 30   AVNELPLNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADVMRKEDADTA 89

Query: 297  DQ---------------------VCSVCLGILQFAYMDNNGVFVKNESASELAATIITD- 410
            D                       C +CLGILQF Y D++G  +K +SA + A  +    
Sbjct: 90   DHPQKDGEFSIKQLHQEPLKETNFCKICLGILQFLYNDDSGTLLKKDSADDFALAVSEPV 149

Query: 411  ----HQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAI 566
                HQ+D+F+L+ S+P  + +N +AVWLY+K ++  +L F E ++     TKD+ K+ +
Sbjct: 150  KQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYRHELWFQEKAEYQFICTKDILKLLV 209

Query: 567  TNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDT-SDTNGFSKRRKTGNVEGIRTVQSC 743
            TN L++ LGVKS++ S +IRLTY+  H+ A T+D   + NG +KRRKTG    ++T +  
Sbjct: 210  TNPLQSLLGVKSSQGSLRIRLTYS--HSDALTRDDIQERNGGNKRRKTGFTGDLKTTEES 267

Query: 744  TAAGEAS-------ADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNP 902
                  S        DGL K    ++     Q    +     L KV++PCCLT  C R+P
Sbjct: 268  VTPHVESFDNHIGDGDGLTKVCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHCCRSP 322

Query: 903  IFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDV 1082
            IFIGGRY K+SRNVSQ+RW+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGREDIDV
Sbjct: 323  IFIGGRYFKYSRNVSQTRWVIDDERMGEASVEEIVGRAILPIFQGDNYKFHAAGREDIDV 382

Query: 1083 RMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGE 1262
            RMLGTGRPFL+EIQNAR+VPS V +K++E +IN+LES+ ++VK+L+ +GS GWDLMREGE
Sbjct: 383  RMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWDLMREGE 442

Query: 1263 AEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVE 1442
            AEKQKQY ALVWISRP++D+DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM+VE
Sbjct: 443  AEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWMKVE 502

Query: 1443 KIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            +I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILG RAEILQLDVTDVKMDCFQ
Sbjct: 503  RISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGRRAEILQLDVTDVKMDCFQ 562

Query: 1623 DE 1628
             E
Sbjct: 563  AE 564


>XP_019186230.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X2
            [Ipomoea nil]
          Length = 553

 Score =  567 bits (1460), Expect = 0.0
 Identities = 297/538 (55%), Positives = 382/538 (71%), Gaps = 32/538 (5%)
 Frame = +3

Query: 102  QEADVLK----AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFN 269
            ++ DVLK    AV  +P NA+KDL S+GVC RCI  + G ++ ICS PS+  S L     
Sbjct: 29   KDKDVLKLISDAVREVPANAIKDLWSIGVCARCIFRVLGLHELICSHPSLSYSVLITVLG 88

Query: 270  QV-LPQGDYADQ-----------------VCSVCLGILQFAYMDNNGVFVKNESASELAA 395
             V +  GD  D                  VC +CLG+LQF + D NG  +K +SA + A+
Sbjct: 89   VVRIEGGDGVDSSAKHELLNIQELTTEANVCKICLGVLQFVFSDENGTLLKKDSAYDFAS 148

Query: 396  TIIT------DHQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSKS----TK 545
             I         HQ+ +F+L+VS+PPLV +N +A+WLY++ K+ S+L F + + S    TK
Sbjct: 149  KIAELVKQQQHHQIGSFSLEVSLPPLVTENDQAIWLYVQKKYESELWFQQKAASQCFSTK 208

Query: 546  DVFKMAITNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDTSDTNGFSKRRKTGNVEGI 725
            DV K+ +   L+T LG+K  E++F IRLTY+    S   Q+ ++ N  +KRRKT +    
Sbjct: 209  DVLKLLVIKPLQTLLGLKHMESTFHIRLTYS---GSLRQQNANERNVGNKRRKTDSAY-T 264

Query: 726  RTVQSCTAAGEASADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPI 905
            ++V      GEA          +   S + Q    +  K+ +EKV QPC LT  CYRNPI
Sbjct: 265  QSVDIQKVEGEAF---------TRTSSLKSQKDCFDYVKLLIEKVIQPCHLTTQCYRNPI 315

Query: 906  FIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVR 1085
            +IGGRYLK+SRNVSQ+RW+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGREDIDVR
Sbjct: 316  YIGGRYLKYSRNVSQTRWVIDDERMGEASVEEILGGCILPLCQGDGYKFHAAGREDIDVR 375

Query: 1086 MLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEA 1265
            MLGTGRPFL+EIQNARQVPS  F+K +E++IN+LE++ +KV++LK LGS GW ++ EGEA
Sbjct: 376  MLGTGRPFLVEIQNARQVPSEGFIKDIEIKINSLENQLVKVRNLKSLGSQGWAMINEGEA 435

Query: 1266 EKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEK 1445
            EKQKQY+ALVWISR +N+ DL  I S+KDMKI+QKTP+RVLHRRSP+ REKIIHWM++E+
Sbjct: 436  EKQKQYSALVWISRDVNNEDLQTISSVKDMKIIQKTPIRVLHRRSPMEREKIIHWMKLER 495

Query: 1446 IAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCF 1619
            + GS+QYFLLHLCTQAGTYIKEFVHGDLGRT+PSIGS+LGCRAEILQLDVTDVKMDCF
Sbjct: 496  LEGSSQYFLLHLCTQAGTYIKEFVHGDLGRTNPSIGSMLGCRAEILQLDVTDVKMDCF 553


>XP_009775534.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana sylvestris] XP_016457703.1 PREDICTED: putative
            tRNA pseudouridine synthase Pus10 [Nicotiana tabacum]
          Length = 567

 Score =  567 bits (1460), Expect = 0.0
 Identities = 298/546 (54%), Positives = 383/546 (70%), Gaps = 43/546 (7%)
 Frame = +3

Query: 120  KAVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDY 293
            +AV  LP+NAVKDL S+G C RC+  L G  + +CS PS+  S        V+ +  GD 
Sbjct: 29   EAVNELPVNAVKDLWSIGACVRCVFRLLGLRELMCSHPSLSISKWCDILEDVMRKEDGDT 88

Query: 294  ADQ---------------------VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT- 407
            ADQ                      C +CLGILQF Y D+NG  +K +SA++ A  I   
Sbjct: 89   ADQPQKDGELSNKQLHQEPLKETNFCKICLGILQFLYNDDNGTLLKKDSANDFALVIAEL 148

Query: 408  ----DHQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMA 563
                 HQ+D+F+L+VS+P  + +  +AV LY+K ++  +L F E ++    STKD+ K+ 
Sbjct: 149  LKQQHHQIDSFSLEVSVPSALAETDQAVRLYMKNRYHHELWFQEKAECQFISTKDILKLL 208

Query: 564  ITNTLETSLGVKSTETSFQIRLTYTLAHTSASTQD-TSDTNGFSKRRKT---GNVEGIRT 731
            + N L+  LGVKS+++SF++RLTY+  H+ A T+D   +  G +KRRKT   G    ++T
Sbjct: 209  VINPLQKLLGVKSSQSSFRVRLTYS--HSDALTRDDVQERAGGNKRRKTDKAGFTGDLKT 266

Query: 732  V-QSCTAAGEA------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLC 890
              +S T   E+        DGL K    ++     Q    +     L KV++PCCLT  C
Sbjct: 267  TKESVTPHVESFDNHIGDGDGLMKLCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHC 321

Query: 891  YRNPIFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGRE 1070
             R+PIFIGGRYLK+SRNVSQ+RW+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGRE
Sbjct: 322  CRSPIFIGGRYLKYSRNVSQTRWVIDDERMGEASVEEIVGGAILPIFQGDNYKFHAAGRE 381

Query: 1071 DIDVRMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLM 1250
            DIDVRMLGTGRPFL+EIQNARQVPS V +K++E +IN++ES+ ++VK+L+ +GS GWDLM
Sbjct: 382  DIDVRMLGTGRPFLVEIQNARQVPSEVLIKEIERKINSVESRLVRVKNLRAVGSQGWDLM 441

Query: 1251 REGEAEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHW 1430
            REGEAEKQKQY ALVWISRP++D DL  I SLK++K+ Q+TP+RVLHRRSPL REKIIHW
Sbjct: 442  REGEAEKQKQYTALVWISRPVSDEDLQTISSLKELKVAQRTPIRVLHRRSPLEREKIIHW 501

Query: 1431 MRVEKIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKM 1610
            M+VE+I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILGCR EILQLDVTDVKM
Sbjct: 502  MKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGCRTEILQLDVTDVKM 561

Query: 1611 DCFQDE 1628
            DCFQ E
Sbjct: 562  DCFQAE 567


>XP_019259999.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana attenuata]
          Length = 567

 Score =  566 bits (1459), Expect = 0.0
 Identities = 301/570 (52%), Positives = 392/570 (68%), Gaps = 44/570 (7%)
 Frame = +3

Query: 45   GRNSLELGSPSPYEKMGGRQEADVLK-AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCI 221
            G  + E+ S     ++   Q+  +L+ AV  LP+NAVKDL S+G C RC+  LFG ++ +
Sbjct: 3    GEVAAEVNSVEGNMQLSEEQKLRLLREAVNELPVNAVKDLWSIGACVRCVFRLFGLHELM 62

Query: 222  CSSPSIPTSALARAFNQVLPQGDY--ADQ---------------------VCSVCLGILQ 332
            CS PS+  S        V+ + D   ADQ                      C++CLGILQ
Sbjct: 63   CSHPSLSISKWCDILEDVMRKEDVDTADQPQKDGELSNKQLHQEPLKETNFCTICLGILQ 122

Query: 333  FAYMDNNGVFVKNESASELAATIIT-----DHQLDTFALQVSIPPLVLDNQKAVWLYIKT 497
            F Y D+NG  +K +SA++ A  I        HQ+D+F+L+VS+P  + +  +AV LY+K 
Sbjct: 123  FLYNDDNGTLLKKDSANDFALVIAELLKQQHHQIDSFSLEVSVPSALAETDQAVRLYMKN 182

Query: 498  KWSSQLEFLELSK----STKDVFKMAITNTLETSLGVKSTETSFQIRLTYTLAHTSASTQ 665
            ++  +L F E ++    STKD+ K  + N L+  LGVKS+++SF++RLTY+  H+ A T+
Sbjct: 183  RYHHELWFQEKAECQFISTKDILKSLVINPLQRLLGVKSSQSSFRVRLTYS--HSDALTR 240

Query: 666  D-TSDTNGFSKRRKTGNV----EGIRTVQSCTAAGEA------SADGLKKYIDSEAVSNE 812
            D   +  G +KRRKT       +   T +S T   E+        DGL K    ++    
Sbjct: 241  DGVQERAGGNKRRKTDKAGFSGDLKTTKESVTPHVESFDNHIGDGDGLTKVCPKDS---- 296

Query: 813  LQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKFSRNVSQSRWIIDDERMGEAS 992
             Q    N     L KV++PCCLT  C R+PIFIGGRYLK+SRNVSQ+RW+IDDERMGEAS
Sbjct: 297  -QDLSCNYLMAQLNKVSKPCCLTIHCCRSPIFIGGRYLKYSRNVSQTRWVIDDERMGEAS 355

Query: 993  VEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFLIEIQNARQVPSSVFMKQVEM 1172
            VEEI+G  +LP+ +G+ YKFHAAGREDIDVRMLGTGRPFL+EIQNARQVPS V +K++E 
Sbjct: 356  VEEIVGGAILPIFQGNNYKFHAAGREDIDVRMLGTGRPFLVEIQNARQVPSEVLIKEIER 415

Query: 1173 RINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAALVWISRPINDNDLHAIVSLKD 1352
            +IN++ES+ ++VK+L+ +GS GWDLMREGEAEKQKQY ALVWISRP++D DL  I SLK+
Sbjct: 416  KINSVESRLVRVKNLRAVGSQGWDLMREGEAEKQKQYTALVWISRPVSDEDLQTISSLKE 475

Query: 1353 MKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFLLHLCTQAGTYIKEFVHGDLG 1532
            +K+ Q+TP+RVLHRRSPL REKIIHWM+VE+I+GS+QY LLHLCTQAGTYIKEFVHGD G
Sbjct: 476  LKVAQRTPIRVLHRRSPLEREKIIHWMKVERISGSSQYLLLHLCTQAGTYIKEFVHGDFG 535

Query: 1533 RTHPSIGSILGCRAEILQLDVTDVKMDCFQ 1622
            RT PS+GSILGCR EILQLDVTDVKMDCFQ
Sbjct: 536  RTQPSVGSILGCRTEILQLDVTDVKMDCFQ 565


>XP_016432382.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Nicotiana tabacum]
          Length = 567

 Score =  566 bits (1458), Expect = 0.0
 Identities = 298/545 (54%), Positives = 381/545 (69%), Gaps = 43/545 (7%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQ--GDYA 296
            AV  LP+NAVKDL S+G C RCI  LFG ++ +CS PS+  S        V+ +   D A
Sbjct: 30   AVNELPLNAVKDLWSIGACARCIFRLFGLHELMCSHPSLSISKWYDILADVMRKEDADTA 89

Query: 297  DQ---------------------VCSVCLGILQFAYMDNNGVFVKNESASELAATIITD- 410
            D                       C +CLGILQF Y D++G  +K +SA + A  +    
Sbjct: 90   DHPQKDGEFSIKQLHQEPLKETNFCKICLGILQFLYNDDSGTLLKKDSADDFALAVSEPV 149

Query: 411  ----HQLDTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLELSK----STKDVFKMAI 566
                HQ+D+F+L+ S+P  + +N +AVWLY+K ++  +L F E ++     TKD+ K+ +
Sbjct: 150  KQQHHQIDSFSLEFSLPSALTENDQAVWLYMKNRYRHELWFQEKAEYQFICTKDILKLLV 209

Query: 567  TNTLETSLGVKSTETSFQIRLTYTLAHTSASTQDT-SDTNGFSKRRKTGNV----EGIRT 731
            TN L++ LGVKS++ S +IRLTY+  H+ A T+D   + NG +KRRKTG      +   T
Sbjct: 210  TNPLQSLLGVKSSQGSLRIRLTYS--HSDALTRDDIQERNGGNKRRKTGKAGFTGDLKTT 267

Query: 732  VQSCTAAGEA------SADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCY 893
             +S T   E+        DGL K    ++     Q    +     L KV++PCCLT  C 
Sbjct: 268  EESVTPHVESFDNHIGDGDGLTKVCPKDS-----QDLSCSYLMAQLNKVSKPCCLTIHCC 322

Query: 894  RNPIFIGGRYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGRED 1073
            R+PIFIGGRY K+SRNVSQ+RW+IDDERMGEASVEEI+G  +LP+ +GD YKFHAAGRED
Sbjct: 323  RSPIFIGGRYFKYSRNVSQTRWVIDDERMGEASVEEIVGRAILPIFQGDNYKFHAAGRED 382

Query: 1074 IDVRMLGTGRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMR 1253
            IDVRMLGTGRPFL+EIQNAR+VPS V +K++E +IN+LES+ ++VK+L+ +GS GWDLMR
Sbjct: 383  IDVRMLGTGRPFLVEIQNARKVPSEVLIKEIERKINSLESQLVRVKNLRAVGSQGWDLMR 442

Query: 1254 EGEAEKQKQYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWM 1433
            EGEAEKQKQY ALVWISRP++D+DL  I SLK++K+ QKTP+RVLHRRSPL REKIIHWM
Sbjct: 443  EGEAEKQKQYTALVWISRPVSDDDLQTISSLKELKVAQKTPIRVLHRRSPLEREKIIHWM 502

Query: 1434 RVEKIAGSTQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMD 1613
            +VE+I+GS+QY LLHLCTQAGTYIKEFVHGD GRT PS+GSILG RAEILQLDVTDVKMD
Sbjct: 503  KVERISGSSQYLLLHLCTQAGTYIKEFVHGDFGRTQPSVGSILGRRAEILQLDVTDVKMD 562

Query: 1614 CFQDE 1628
            CFQ E
Sbjct: 563  CFQAE 567


>CBI18893.3 unnamed protein product, partial [Vitis vinifera]
          Length = 591

 Score =  567 bits (1460), Expect = 0.0
 Identities = 300/531 (56%), Positives = 373/531 (70%), Gaps = 18/531 (3%)
 Frame = +3

Query: 90   MGGRQEADVLK-AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAF 266
            + G  +  +L  AV  +  +AVKDL+SLG+C  CI  L G    I S PS+  S L    
Sbjct: 61   IAGEDDMRILNDAVSKVSPHAVKDLLSLGICIGCIFRLSGVRGHIFSCPSLLLSILHPVI 120

Query: 267  NQVLPQGDYADQ----VCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQL 419
            +          +    +CS+CLG+LQF Y D+  + VK +SA ELA +I        H+ 
Sbjct: 121  DSGAAHSSQESECEPVICSICLGVLQFTYFDDKKMLVKKDSADELAVSIANLVKKEGHEF 180

Query: 420  DTFALQVSIPPLVLDNQKAVWLYIKTKWSSQLEFLE--LSK--STKDVFKMAITNTLETS 587
            D F+L+VSIPP++ +N+  V LY+K K+ S+L F E  LS+  S KD  K+++ N LE  
Sbjct: 181  DNFSLEVSIPPVIAENEHVVLLYMKKKYGSELWFQEKFLSERISAKDALKLSLCNPLEKL 240

Query: 588  LGVKSTETS-FQIRLTYTLAHTSASTQDTSDTNGFSKRRKTGNVEGIRTVQSCTAAGEAS 764
            L VKS+ +S F+IRLTY     S   Q+ ++ +   KRR+TG      TV      G   
Sbjct: 241  LDVKSSGSSTFRIRLTYAQPRASGKNQNITERSQGCKRRRTGTENIFSTVHGSVVDGIEC 300

Query: 765  ADGLKKYIDSEAVSNELQVQGINN---FKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKFS 935
            +D      D+        +Q   N   FK PLEKVNQPC L +LCYR  I+IGGRYLK+S
Sbjct: 301  SDISTSEADAGIRKPSDGLQDNENSDCFKFPLEKVNQPCHLAFLCYRTHIYIGGRYLKYS 360

Query: 936  RNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFLI 1115
            RNVSQ+RWIIDDERMGEASVEEIIG  +LPM  GD YKFHAAGREDI+VRMLG+GRPFL+
Sbjct: 361  RNVSQTRWIIDDERMGEASVEEIIGGNILPMCLGDNYKFHAAGREDINVRMLGSGRPFLV 420

Query: 1116 EIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAALV 1295
            EIQN+R VPS   +K++E++INNLE+K ++VK+LK + S+GW LM+EGE EKQKQYAALV
Sbjct: 421  EIQNSRHVPSEALIKEIEVKINNLENKLVRVKNLKLVDSHGWSLMQEGEEEKQKQYAALV 480

Query: 1296 WISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFLL 1475
            WI RP+ D D+ AI SLKD KI+Q+TP+RVLHRRSPL REKIIHWM++EKIAGS+QYFLL
Sbjct: 481  WIDRPLKDEDMQAISSLKDKKILQRTPIRVLHRRSPLEREKIIHWMKIEKIAGSSQYFLL 540

Query: 1476 HLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            HLCTQAGTYIKEFVHGDLGRTHPS+GSILGCRAEILQLDVT+VKMDCF DE
Sbjct: 541  HLCTQAGTYIKEFVHGDLGRTHPSVGSILGCRAEILQLDVTEVKMDCFLDE 591


>EOY15171.1 Pseudouridine synthase family protein, putative isoform 1 [Theobroma
            cacao]
          Length = 548

 Score =  563 bits (1452), Expect = 0.0
 Identities = 298/518 (57%), Positives = 377/518 (72%), Gaps = 16/518 (3%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLP-QGDYAD 299
            AV SLP + VK+L+S+GVC RC+L LFG  + I    S   S L     +    + +   
Sbjct: 32   AVRSLPYHVVKELLSVGVCARCVLRLFGIEEHIYCHSSFSQSMLGSILEEETSFKSEVEF 91

Query: 300  QVCSVCLGILQFAYMDNNG-VFVKNESASELAATIIT-----DHQLDTFALQVSIPPLVL 461
            Q CSVCLG+LQF+Y D+     VK ESA ++A +I        HQ+D F+L+VS+PPL+L
Sbjct: 92   QFCSVCLGVLQFSYCDDGEETVVKKESAKDMALSIAELVKQEGHQIDGFSLEVSVPPLIL 151

Query: 462  DNQKAVWLYIKTKWSSQLEFLE--LSK--STKDVFKMAITNTLETSLGVKSTETSFQIRL 629
            +N+ ++  ++K K+ S+  F E  LSK  S KDV K AIT  LET LGVK++ +SF+IRL
Sbjct: 152  ENESSLLSHMKKKYESEPWFQERLLSKCHSVKDVLKFAITKPLETLLGVKASASSFRIRL 211

Query: 630  TYTLAHTSASTQDTSDTNGFSKRRKTGNVEGIRTVQSCTAAGEASADGLKKYIDSEAV-- 803
            TYT    S   Q++ + N   KRRKTG    +  V   +   +    G    I++ +V  
Sbjct: 212  TYTHKRASKKVQNSVERNQECKRRKTGTENALHNVNDESVIADKDCSGYST-IETNSVTG 270

Query: 804  --SNELQVQGIN-NFKIPLEKVNQPCCLTYLCYRNPIFIGGRYLKFSRNVSQSRWIIDDE 974
              SN +Q Q  + +F+ P+EKV++PC L Y+C R  I++ GRYLK+SRNVSQ+RWIID+E
Sbjct: 271  IFSNGVQDQETSEHFESPIEKVSEPCHLVYVCCRTHIYLCGRYLKYSRNVSQTRWIIDEE 330

Query: 975  RMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGTGRPFLIEIQNARQVPSSVF 1154
            RMGEASVEEIIG+ +LP+ +GD YKFHAAGREDIDVRMLG+GRPFL+EIQNARQVPS + 
Sbjct: 331  RMGEASVEEIIGSNILPICQGDNYKFHAAGREDIDVRMLGSGRPFLLEIQNARQVPSELI 390

Query: 1155 MKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQKQYAALVWISRPINDNDLHA 1334
            +K++E +IN LE+K + VK+LK +GS GW LM EGEAEKQKQY ALVWISRP+ D D+  
Sbjct: 391  VKEMESKINGLENKSVGVKNLKVVGSQGWALMHEGEAEKQKQYCALVWISRPLEDEDMQT 450

Query: 1335 IVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGSTQYFLLHLCTQAGTYIKEF 1514
            I  L DMKI+Q+TP+RVLHRRSPL REKIIHWM++E+IAGS+QYFLLHLCTQAGTYIKEF
Sbjct: 451  ISLLNDMKILQRTPIRVLHRRSPLEREKIIHWMKIERIAGSSQYFLLHLCTQAGTYIKEF 510

Query: 1515 VHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            VHGDLGRT PS+GSILGCRAEILQLDVTDVKMDCF  E
Sbjct: 511  VHGDLGRTQPSVGSILGCRAEILQLDVTDVKMDCFVTE 548


>XP_011041278.1 PREDICTED: putative tRNA pseudouridine synthase Pus10 isoform X1
            [Populus euphratica]
          Length = 560

 Score =  561 bits (1447), Expect = 0.0
 Identities = 305/537 (56%), Positives = 373/537 (69%), Gaps = 35/537 (6%)
 Frame = +3

Query: 123  AVGSLPINAVKDLVSLGVCHRCILSLFGCYDCICSSPSIPTSALARAFNQVLPQGDYAD- 299
            AV SLP +AVKDL+S GVC RCI  LFG  D      S+  S +      +L +GD +  
Sbjct: 41   AVRSLPSHAVKDLLSAGVCMRCIFRLFGISDHAYFRSSLSPSVMCN----ILGEGDSSSS 96

Query: 300  ---------QVCSVCLGILQFAYMDNNGVFVKNESASELAATIIT-----DHQLDTFALQ 437
                     +VC +CLGILQF Y D   + VK ++  ELAA+I        HQ++ F+L+
Sbjct: 97   ESMESELKPEVCRICLGILQFIYRDEKEMLVKRKNGDELAASIAELIRQEGHQINGFSLE 156

Query: 438  VSIPPLVLDNQKAVWLYIKTKWSSQLEFLE-LSK--STKDVFKMAITNTLETSLGVKSTE 608
            VSIPP++ +N++AV LY+K K+ S+L F E LS+  STKD  K AI N LE  L VKS  
Sbjct: 157  VSIPPIIFENEQAVRLYMKKKYGSELWFQENLSECISTKDALKFAIVNPLEIILDVKSGP 216

Query: 609  TSFQIRLTYTLAHTSASTQDTSDTNGFSKRRKTGNVEGIRTV-----------------Q 737
            +  +IRLTYT    S     + D NG S+RRKTG   G+ TV                 Q
Sbjct: 217  SYVRIRLTYTETKPSIIPLKSVDRNGGSQRRKTGTNNGLDTVHVELVGNGTGCCDLSLGQ 276

Query: 738  SCTAAGEASADGLKKYIDSEAVSNELQVQGINNFKIPLEKVNQPCCLTYLCYRNPIFIGG 917
               A+G +S  GL ++  SE  +            + LE+VN+PC L +LCYR PI+ GG
Sbjct: 277  GSPASGRSST-GLAEHESSECSN------------LHLERVNEPCHLVFLCYRTPIYFGG 323

Query: 918  RYLKFSRNVSQSRWIIDDERMGEASVEEIIGNIVLPMSKGDTYKFHAAGREDIDVRMLGT 1097
            RYLKFSRNVSQ+RWIIDDERMGEAS+EEIIG  +LPM +GD+YKFHAAGREDIDVRMLG+
Sbjct: 324  RYLKFSRNVSQTRWIIDDERMGEASIEEIIGGNILPMCQGDSYKFHAAGREDIDVRMLGS 383

Query: 1098 GRPFLIEIQNARQVPSSVFMKQVEMRINNLESKRIKVKDLKCLGSNGWDLMREGEAEKQK 1277
            GRPFL+E+QNAR+VPS   +K++E R+NNL +K + VK+L  + S+ WDLMREGEAEKQK
Sbjct: 384  GRPFLVEVQNARKVPSEALVKEIETRMNNLGNKLVGVKNLSLVTSHSWDLMREGEAEKQK 443

Query: 1278 QYAALVWISRPINDNDLHAIVSLKDMKIMQKTPVRVLHRRSPLGREKIIHWMRVEKIAGS 1457
            QY ALVWISRP+ D DL  I S KD++I+Q+TPVRVLHRRSPL R KIIHWM++E+I GS
Sbjct: 444  QYCALVWISRPLEDEDLQCIASHKDLQILQRTPVRVLHRRSPLERRKIIHWMKIEQITGS 503

Query: 1458 TQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILGCRAEILQLDVTDVKMDCFQDE 1628
            +QYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSIL CRAEILQLDVTDVKMDCF  E
Sbjct: 504  SQYFLLHLCTQAGTYIKEFVHGDLGRTHPSIGSILRCRAEILQLDVTDVKMDCFLAE 560