BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum23_contig00004265
         (2466 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

GAV66835.1 MS_channel domain-containing protein [Cephalotus foll...   346   0.0  
XP_002514042.1 PREDICTED: mechanosensitive ion channel protein 3...   347   0.0  
XP_012068009.1 PREDICTED: mechanosensitive ion channel protein 3...   349   0.0  
CDP11503.1 unnamed protein product [Coffea canephora]                 337   0.0  
XP_018633012.1 PREDICTED: mechanosensitive ion channel protein 3...   342   0.0  
XP_009624658.1 PREDICTED: mechanosensitive ion channel protein 3...   342   0.0  
XP_011016935.1 PREDICTED: mechanosensitive ion channel protein 3...   350   0.0  
XP_006338061.1 PREDICTED: mechanosensitive ion channel protein 3...   341   0.0  
XP_016460352.1 PREDICTED: mechanosensitive ion channel protein 3...   342   0.0  
XP_009785624.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_009785640.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_010254532.1 PREDICTED: mechanosensitive ion channel protein 2...   347   0.0  
XP_016481428.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_016481429.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_002302344.2 hypothetical protein POPTR_0002s10610g [Populus t...   350   0.0  
XP_018633013.1 PREDICTED: mechanosensitive ion channel protein 2...   342   0.0  
XP_015073682.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_016460353.1 PREDICTED: mechanosensitive ion channel protein 2...   342   0.0  
XP_009785633.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  
XP_019266973.1 PREDICTED: mechanosensitive ion channel protein 3...   340   0.0  

>GAV66835.1 MS_channel domain-containing protein [Cephalotus follicularis]
          Length = 712

 Score =  346 bits (887), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 186/214 (86%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+YTAVWVAA SLFMELLGFSTQ+W               REIFTNFLSS+M
Sbjct: 231  MGFSFAGKAIYTAVWVAAVSLFMELLGFSTQRWLTAGGLGTVLLTLAGREIFTNFLSSVM 290

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF++N+WIQTKIEGYEVSGTVEHVGWWSPT+IRGDDREAVHIPNHKFTV+VVRNL
Sbjct: 291  IHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVSVVRNL 350

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKT+LA+SHLDVNK+N IVADMRKVL+KNPQ+EQ++LHRRVFL+NI+PENQ+L
Sbjct: 351  SQKTHWRIKTYLAISHLDVNKVNNIVADMRKVLAKNPQVEQQRLHRRVFLENINPENQSL 410

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             ILVSCFVKTS FEEYLCVKEA+   +  ++  H
Sbjct: 411  MILVSCFVKTSHFEEYLCVKEAILLDLLRVISHH 444



 Score =  234 bits (597), Expect(3) = 0.0
 Identities = 118/209 (56%), Positives = 146/209 (69%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKGMDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAHG 411
           M     + L REL+I   +GC      + K  LH +NI LSS  LRQD+WS +LLS   G
Sbjct: 1   MTLVGFMRLSRELRIHDKHGCNNHENSVLKGRLHFVNINLSSQALRQDAWSFHLLSRLRG 60

Query: 412 PLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVGLIV 591
           P+C VP R +V  CRS+L PG   EIP+LK A+ +L R    L  +PI+L+L+PAV +I 
Sbjct: 61  PICPVPTRCNVFFCRSVLLPGGGNEIPLLKSAAVVLTRLYDVLSRNPIVLKLIPAVSIIA 120

Query: 592 FAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPVILP 771
           F  WG+ PL+  G +IFL+K D+NW+ S    +L  YLQP+LLW GATLICRALDPV+LP
Sbjct: 121 FVAWGLGPLMRLGMTIFLHKTDSNWKRSRAHYVLTSYLQPLLLWTGATLICRALDPVVLP 180

Query: 772 SAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           S ASQAVKQRLLNFVRSLSTV+AFAYCLS
Sbjct: 181 SEASQAVKQRLLNFVRSLSTVMAFAYCLS 209



 Score =  171 bits (434), Expect(3) = 0.0
 Identities = 119/272 (43%), Positives = 159/272 (58%), Gaps = 21/272 (7%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSDIFTRSRAAANHPFLLIEPSYKINGDEKSKV 1654
            SHHRARLATPIRTVQ++Y E ++D+ PF+D+FTRSRAAA+ P+LLIE S K N D K+K 
Sbjct: 442  SHHRARLATPIRTVQKIYNEADLDNIPFADMFTRSRAAASRPYLLIESSNKTNVDGKAKA 501

Query: 1655 SPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADG---TLGSGSAGVANTMAKVAHS 1825
            S RSV++++EK+  ++A   PDA A++ + V T   D       S S+      A+++ S
Sbjct: 502  SNRSVRTNEEKEGKVEATSTPDAKADAKV-VPTSIPDSIRDKTSSNSSTNPKVSARLSDS 560

Query: 1826 -SAAELPATGSVNAS---QGRS----EKQLNELVAEE-PPVGESLRHSK--DSISGGEST 1972
             S   +P     N S   Q  S    +KQ+ EL +EE  PVG     S+  +  SG E T
Sbjct: 561  QSRTAVPDKSVQNKSDMMQSESIVDAQKQMMELNSEEITPVGAISEKSEVTNRESGCEQT 620

Query: 1973 ------SQTRPDSEKISPSPAV-RPVLEDNIVLGVALEGSKRTLPIXXXXXXXXXXTETK 2131
                  +Q + D E+   SP+V RP LE+NIVLGVALEGSKRTLP+          TE K
Sbjct: 621  DTPSAVTQAKQDGERSVASPSVARPPLEENIVLGVALEGSKRTLPMEEEMTQSPLPTELK 680

Query: 2132 ELATSRNGSVPTAITVDKEDQGGSIPNTSSGD 2227
            ELA SRNG   +     K  Q  ++P T+  D
Sbjct: 681  ELAASRNGGGSSVTEDKKNGQMPAVPGTTQSD 712


>XP_002514042.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic
            [Ricinus communis] EEF48625.1 conserved hypothetical
            protein [Ricinus communis]
          Length = 705

 Score =  347 bits (891), Expect(3) = 0.0
 Identities = 165/203 (81%), Positives = 180/203 (88%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+Y+AVW+AA SLFMELLGFSTQKW               REI TNFLSS+M
Sbjct: 234  MGFSFAGKAVYSAVWIAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF++N+WIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTV VVRNL
Sbjct: 294  IHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVNVVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNK+N IVADMRKVL+KNPQ+EQ++LHRRVFLDNI+PENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKVNNIVADMRKVLAKNPQVEQQRLHRRVFLDNINPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAV 1469
             ILVSCFVKTS FEEYLCVKEAV
Sbjct: 414  MILVSCFVKTSHFEEYLCVKEAV 436



 Score =  224 bits (570), Expect(3) = 0.0
 Identities = 114/212 (53%), Positives = 149/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSK---GMDKCHLHPLNIPLSSNYLRQDSWSLYLLSS 402
           M    S+ L +ELKI  + GC  Q     G  K  L   N+ LSS+ LRQD+W+L+ LSS
Sbjct: 1   MTCTGSIQLSQELKIYTSRGCGSQQNTVLGKGKSRLFLANVTLSSHGLRQDAWTLHFLSS 60

Query: 403 AHGPLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
            H P+   P R +V +C+SLL      EIP++K A+ +L RS  +L GSP++L+L+PA+ 
Sbjct: 61  MHQPIRPAPSRCNVLVCQSLLTSQGGTEIPLVKSAAVVLTRSYNALSGSPLVLKLIPAIS 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           +I FA WG+ PL+  GR IFL+ +DNNW+ S+T  +L  YLQP+LLW GA L+CRALDPV
Sbjct: 121 IIAFASWGLGPLLRLGRIIFLHGSDNNWKKSSTHYVLTSYLQPLLLWTGAILVCRALDPV 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           +L S A+QAVKQRLLNFVRSLSTV+AFAYCLS
Sbjct: 181 VLQSEANQAVKQRLLNFVRSLSTVMAFAYCLS 212



 Score =  176 bits (445), Expect(3) = 0.0
 Identities = 112/271 (41%), Positives = 150/271 (55%), Gaps = 20/271 (7%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ++Y E ++D+ PF+D IF RSRAA N P LLIEPSYKINGD+K+K
Sbjct: 445  SHHRARLATPIRTVQKIYNEADLDNVPFADTIFARSRAATNRPLLLIEPSYKINGDDKTK 504

Query: 1652 VSPRSVQSSQEKDANIDA---------------PLKPDAVAESTLAVGTEKADGTLGSGS 1786
             S +SV +++EKD N++A                LK D VA +T++  +   +   GS +
Sbjct: 505  ASTQSVSTNEEKDGNVEAASTLADAKAGSSPIVELKRDKVAATTISDSSITPE---GSAT 561

Query: 1787 AGVANTMAKVAHSSAAELPATGSVNASQGRSEKQLNE---LVAEEPPVGESLRHSKDSIS 1957
                + + K  H     +P         G  E   NE   L +E+  +G S      +IS
Sbjct: 562  TASDSQLGKSEHE--ISVPKNAETQEPSGSMEGSRNEKMGLNSEDLTLGRSTSEEYSAIS 619

Query: 1958 GGESTSQTRPDSEKISPSPAVRPVLEDNIVLGVALEGSKRTLPIXXXXXXXXXXTETKEL 2137
              +   +       ++P P  RP LE+NIVLGVALEGSKRTLPI          +E+KEL
Sbjct: 620  QAQEAVE-----RSVTPPPVSRPPLEENIVLGVALEGSKRTLPIEDEMDPSPFSSESKEL 674

Query: 2138 ATSRNGSVPTAITVDKED-QGGSIPNTSSGD 2227
            A SRNG        DK+D Q  ++P+ S  D
Sbjct: 675  AASRNGGGSLTAGKDKKDSQMPTVPSASQVD 705


>XP_012068009.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Jatropha curcas] XP_012068010.1 PREDICTED:
            mechanosensitive ion channel protein 3,
            chloroplastic-like isoform X1 [Jatropha curcas]
            XP_012068011.1 PREDICTED: mechanosensitive ion channel
            protein 3, chloroplastic-like isoform X1 [Jatropha
            curcas] KDP41480.1 hypothetical protein JCGZ_15887
            [Jatropha curcas]
          Length = 691

 Score =  349 bits (896), Expect(3) = 0.0
 Identities = 166/214 (77%), Positives = 184/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+Y+AVWVAA SLFMELLGFSTQKW               REIFTNFLSS+M
Sbjct: 234  MGFSFAGKAVYSAVWVAAVSLFMELLGFSTQKWVTAGGLGTVLLTLAGREIFTNFLSSVM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LN+WIQTKIEGYEVSGTVEHVGWWSPT+IRGDDREAVHIPNHKFT+ VVRNL
Sbjct: 294  IHATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTMNVVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQ+EQ+KLHRRVFLDNI+PENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRVFLDNINPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             + VSCFVKTS FEEYLCVKEA+   +  ++  H
Sbjct: 414  MVFVSCFVKTSHFEEYLCVKEAIILDLLRVISHH 447



 Score =  236 bits (601), Expect(3) = 0.0
 Identities = 118/212 (55%), Positives = 149/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSK---GMDKCHLHPLNIPLSSNYLRQDSWSLYLLSS 402
           M  ASS+ L  EL I  + GC+ Q+    G     +H +N+ LSS  LRQDSW+L+ LSS
Sbjct: 1   MTRASSIQLSHELWIHSSQGCYGQNTKIIGKGTNRVHVVNVNLSSRSLRQDSWTLHFLSS 60

Query: 403 AHGPLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
            H P+C  P   +  +CRSLL PG    IP+LK AS +L RS  +L GSP++L+L+PAVG
Sbjct: 61  THRPICHAPSSCNAFVCRSLLSPGGGNGIPLLKSASVVLSRSYDALRGSPVVLKLIPAVG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           +I FA WG+ PL+   R  FL+K+D NW  S++  +L  YLQP+LLW GA LICRALDPV
Sbjct: 121 IIAFAAWGLGPLMRLSRIFFLHKSDKNWSKSSSHYVLTSYLQPLLLWAGAILICRALDPV 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           +L S ASQAVKQRLLNF+RSLSTVLAFAYC+S
Sbjct: 181 VLQSEASQAVKQRLLNFIRSLSTVLAFAYCIS 212



 Score =  161 bits (407), Expect(3) = 0.0
 Identities = 107/246 (43%), Positives = 147/246 (59%), Gaps = 6/246 (2%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ++Y E ++ +   +D I TRS A AN P LLIEPSYKINGD+K+K
Sbjct: 445  SHHRARLATPIRTVQKIYNEADLANVRSADTILTRSGAVANRPLLLIEPSYKINGDDKTK 504

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
             S RSV ++ EKDA  +A    ++ A++  A  T     + G  +       +K+ H +S
Sbjct: 505  ASSRSVCTNGEKDAKAEAASTSESKADAN-AGSTTNTPESKGLATPASDRWQSKMVHDNS 563

Query: 1829 AAELPATGSVNASQGRSEKQLNELVAEEPPVGESL--RHSKDSISGGEST-SQTRPDSEK 1999
                P T   + S   + K+   L +E+  +G +    +S+   S   ST SQ + D E+
Sbjct: 564  VPNNPETKKTSESIEDTWKENMGLNSEDITLGRAAPEEYSESKRSEIPSTISQAKQDVER 623

Query: 2000 ISPSPAV-RPVLEDNIVLGVALEGSKRTLPIXXXXXXXXXXTETKELATSRNGSVPTAIT 2176
             + S +V RP LE+NIVLGVALEGSKRTLPI          +E+KELA SRNG  P ++ 
Sbjct: 624  SAASASVARPPLEENIVLGVALEGSKRTLPIEEEVAPSPFPSESKELAASRNGGGPPSVG 683

Query: 2177 VDKEDQ 2194
             DK+D+
Sbjct: 684  KDKKDE 689


>CDP11503.1 unnamed protein product [Coffea canephora]
          Length = 706

 Score =  337 bits (865), Expect(3) = 0.0
 Identities = 164/214 (76%), Positives = 181/214 (84%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSS M
Sbjct: 237  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSAM 296

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GYEVSGTVEHVGWWSPTVIRGDDREAVHIP+HKFT+ VVRNL
Sbjct: 297  IHATRPFVLNDWIQTKIDGYEVSGTVEHVGWWSPTVIRGDDREAVHIPSHKFTMNVVRNL 356

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIK+ LA+SHLDVNKIN IV+DMRKVL+KNPQIEQ+KLHRRVFLD ID ENQAL
Sbjct: 357  SQKTHWRIKSSLAISHLDVNKINNIVSDMRKVLAKNPQIEQQKLHRRVFLDYIDTENQAL 416

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I+VSCFVKTS FEEYLCVKE++   +  ++  H
Sbjct: 417  MIMVSCFVKTSWFEEYLCVKESILQDLLRVISHH 450



 Score =  230 bits (587), Expect(3) = 0.0
 Identities = 118/215 (54%), Positives = 152/215 (70%), Gaps = 6/215 (2%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCF------FQSKGMDKCHLHPLNIPLSSNYLRQDSWSLYL 393
           MAAA+SLL P E++I  + G F        S  M+K  LH  +  LSS+ +RQD+WSLY+
Sbjct: 1   MAAATSLLSPCEMRICGHYGQFKVHGGLLHSGMMEKTRLHSFSSSLSSHSVRQDAWSLYV 60

Query: 394 LSSAHGPLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLP 573
           L + HGPLC    + +   CRSLLKPG   EIPVLK A+ ++ RS  +L GSP +L L+P
Sbjct: 61  LKNVHGPLCPNNAKFNGFTCRSLLKPGGGNEIPVLKAAAKIISRSHGTLHGSPPVLHLVP 120

Query: 574 AVGLIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRAL 753
           A+G+I FA WG+ PL+ FGR +FL+KND +W+ +   N++  YLQP+L W GA LICRAL
Sbjct: 121 AIGIIAFAAWGLGPLMRFGRILFLSKNDGSWKKTGIHNLVTSYLQPLLFWTGAMLICRAL 180

Query: 754 DPVILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           DPVIL +  SQAVK+RLL FVRSLST+LAFAYCLS
Sbjct: 181 DPVILTTVTSQAVKRRLLYFVRSLSTILAFAYCLS 215



 Score =  174 bits (442), Expect(3) = 0.0
 Identities = 113/264 (42%), Positives = 147/264 (55%), Gaps = 7/264 (2%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ+VY E +V+  PFS+ IF R+RAA+N PFLL++PSYK+N D K +
Sbjct: 448  SHHRARLATPIRTVQKVYGETDVETIPFSETIFRRTRAASNRPFLLVDPSYKVNNDNKIR 507

Query: 1652 VSPRSVQSSQEKDANIDAP----LKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVA 1819
                + Q ++EKD  ++A      K DA       V  +K D    +      N  A V 
Sbjct: 508  TKGHTEQPNEEKDEKVEATSTSETKVDAKVGPASIVDDDKVDDEGSTELISDNNQKATVE 567

Query: 1820 HSSAAE-LPATGSVNASQGRSEKQLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSE 1996
             +S ++ LP  GS++  Q  S KQ  +     P V E     +D  +   STSQ  PD E
Sbjct: 568  RASTSDALPHMGSIDPGQRNSVKQRADRA--RPSVQE---FGRDGTAVSSSTSQAMPDVE 622

Query: 1997 KISPSP-AVRPVLEDNIVLGVALEGSKRTLPIXXXXXXXXXXTETKELATSRNGSVPTAI 2173
            +  PS  A +  LEDNIVLGVALEGSK TLPI          +E KE A   NG+    +
Sbjct: 623  RHIPSASAPKTTLEDNIVLGVALEGSKMTLPIEEDMMPTPTQSEVKEAAACWNGNGLATV 682

Query: 2174 TVDKEDQGGSIPNTSSGDQREKER 2245
              DK+D+ G IPN    D +EKE+
Sbjct: 683  GKDKQDEIGEIPNGGPRDLQEKEK 706


>XP_018633012.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Nicotiana tomentosiformis]
          Length = 724

 Score =  342 bits (876), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 250  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 309

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 310  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 369

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 370  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 429

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 430  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 463



 Score =  216 bits (550), Expect(3) = 0.0
 Identities = 118/224 (52%), Positives = 150/224 (66%), Gaps = 3/224 (1%)
 Frame = +1

Query: 196 GSFFRFFCFP*NMAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQ 372
           G  F F      MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQ
Sbjct: 5   GEAFFFLTVGQTMAAAVSLLLPRELGIHGKCGFTCQPKGTMSRIQTDSCSKFLSSHSSRQ 64

Query: 373 DSWSLYLLSSAHGPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEG 546
           D+WS+YLL+S  GPL     P R +V LCRSLLKPG   E   LK A+ +  RS+  + G
Sbjct: 65  DAWSIYLLNSVRGPLFTHPSPTRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNIIHG 124

Query: 547 SPIILQLLPAVGLIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWI 726
           SP++LQL+PA+G+IV A WG+ PL+ FGR  F +++ +NW+ S   ++ + YL+P+LLW 
Sbjct: 125 SPLVLQLVPAIGVIVLAAWGLIPLMSFGRKFFFHESGSNWKQSGLHHVADSYLKPVLLWT 184

Query: 727 GATLICRALDPVILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           GA LICRA+DP+ILPSAASQAVKQR   F+RSLSTV+A AYCLS
Sbjct: 185 GAILICRAVDPLILPSAASQAVKQRFTTFIRSLSTVMALAYCLS 228



 Score =  180 bits (457), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 155/269 (57%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 461  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDRP--NRPFFLIEPSYRINNDDKTK 518

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
            VS RSVQ  +EKD   +AP    A  ++   +        +   SA  AN   KVA  SS
Sbjct: 519  VSARSVQPDEEKDGKAEAPSTSQAADDTNGLLSKPTEKEVVKVSSASNANVDLKVATLSS 578

Query: 1829 AAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-I 2002
              + P  GS++  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ I
Sbjct: 579  DGKTPKQGSISPVKSNSEKNQVASATGEAPGLTSDINIEKTDVA--SAASQAQQDAERPI 636

Query: 2003 SPSPAVRPVLEDNIVLGVALEGSKRTLPI-------XXXXXXXXXXTETKELATSRNGSV 2161
            SP    RP+LEDNIVLGVALEGSK TLPI                 +E+KELA  RNG+ 
Sbjct: 637  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEEATPPSPSPSPTFSDSESKELAACRNGNS 696

Query: 2162 PTAITVDK-EDQGGSIPNTSSGDQREKER 2245
             T+   DK +DQ    P+  S  Q+E+ER
Sbjct: 697  STSSNKDKMDDQMAEAPSAPS-VQKERER 724


>XP_009624658.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X3 [Nicotiana tomentosiformis] XP_018633014.1
            PREDICTED: mechanosensitive ion channel protein 3,
            chloroplastic-like isoform X3 [Nicotiana tomentosiformis]
            XP_018633015.1 PREDICTED: mechanosensitive ion channel
            protein 3, chloroplastic-like isoform X3 [Nicotiana
            tomentosiformis]
          Length = 708

 Score =  342 bits (876), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 414  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 447



 Score =  214 bits (545), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 147/212 (69%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIHGKCGFTCQPKGTMSRIQTDSCSKFLSSHSSRQDAWSIYLLNSVR 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P R +V LCRSLLKPG   E   LK A+ +  RS+  + GSP++LQL+PA+G
Sbjct: 61  GPLFTHPSPTRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNIIHGSPLVLQLVPAIG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           +IV A WG+ PL+ FGR  F +++ +NW+ S   ++ + YL+P+LLW GA LICRA+DP+
Sbjct: 121 VIVLAAWGLIPLMSFGRKFFFHESGSNWKQSGLHHVADSYLKPVLLWTGAILICRAVDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR   F+RSLSTV+A AYCLS
Sbjct: 181 ILPSAASQAVKQRFTTFIRSLSTVMALAYCLS 212



 Score =  180 bits (457), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 155/269 (57%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 445  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDRP--NRPFFLIEPSYRINNDDKTK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
            VS RSVQ  +EKD   +AP    A  ++   +        +   SA  AN   KVA  SS
Sbjct: 503  VSARSVQPDEEKDGKAEAPSTSQAADDTNGLLSKPTEKEVVKVSSASNANVDLKVATLSS 562

Query: 1829 AAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-I 2002
              + P  GS++  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ I
Sbjct: 563  DGKTPKQGSISPVKSNSEKNQVASATGEAPGLTSDINIEKTDVA--SAASQAQQDAERPI 620

Query: 2003 SPSPAVRPVLEDNIVLGVALEGSKRTLPI-------XXXXXXXXXXTETKELATSRNGSV 2161
            SP    RP+LEDNIVLGVALEGSK TLPI                 +E+KELA  RNG+ 
Sbjct: 621  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEEATPPSPSPSPTFSDSESKELAACRNGNS 680

Query: 2162 PTAITVDK-EDQGGSIPNTSSGDQREKER 2245
             T+   DK +DQ    P+  S  Q+E+ER
Sbjct: 681  STSSNKDKMDDQMAEAPSAPS-VQKERER 708


>XP_011016935.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Populus euphratica]
          Length = 708

 Score =  350 bits (897), Expect(3) = 0.0
 Identities = 168/214 (78%), Positives = 185/214 (86%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+YTAVW+AA SLFMELLGFSTQKW               REIFTNFLSSIM
Sbjct: 229  MGFSFAGKAVYTAVWIAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIM 288

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LN+WIQTKIEGYEVSGTVEHVGWWSPT+IRGDDREAVHIPNHKF+V++VRNL
Sbjct: 289  IHATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFSVSIVRNL 348

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDV KIN IVADMRKVL+KNPQIEQ++LHRRVFLDNI+PENQAL
Sbjct: 349  SQKTHWRIKTHLAISHLDVKKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNINPENQAL 408

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             ILVSCFVKTS FEEYLCVKEAV   +  ++  H
Sbjct: 409  MILVSCFVKTSHFEEYLCVKEAVLLDLLRVISHH 442



 Score =  224 bits (571), Expect(3) = 0.0
 Identities = 114/209 (54%), Positives = 146/209 (69%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKGMDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAHG 411
           M   SS+ L  EL++   +GC  Q     +  L  +N  LSS +LRQD W L+ L+S  G
Sbjct: 1   MTRVSSMTLSHELRVRNCHGCNNQYTSASRVQL--VNANLSSQFLRQDPWGLHFLNSIRG 58

Query: 412 PLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVGLIV 591
           P   VP R +V LCRS+L  G   EIPVLK A+    RS  +L GS ++L+L+PA+G+I 
Sbjct: 59  PPSPVPSRCNVFLCRSVLTSGGGNEIPVLKSAALAFTRSYDALRGSNLVLKLIPAIGIIT 118

Query: 592 FAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPVILP 771
           FA WG+ PL+  GR+IFL+K+DN+W+ S+T  ++  YLQP+L W GATLICRALDPV+L 
Sbjct: 119 FAAWGLGPLIWLGRTIFLHKSDNSWKKSSTHYVMTSYLQPLLFWTGATLICRALDPVVLQ 178

Query: 772 SAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           S  SQAVKQRLLNFVRSLSTV+AFAYCLS
Sbjct: 179 SEVSQAVKQRLLNFVRSLSTVVAFAYCLS 207



 Score =  162 bits (409), Expect(3) = 0.0
 Identities = 109/272 (40%), Positives = 152/272 (55%), Gaps = 29/272 (10%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ++Y E ++++ PFSD IFTRS A ANHP LL+EPSYKIN ++K K
Sbjct: 440  SHHRARLATPIRTVQKIYSEADLENVPFSDSIFTRSGATANHPLLLVEPSYKINSEDKVK 499

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAES---TLAVGTEKADGTLGSGSAGVANTMAKVAH 1822
             S RS+++++EKDA ++A L  +  A++   ++ V   K D  +   ++  ++T +KV+ 
Sbjct: 500  ASNRSLRANEEKDAKVEAALVSELKADTKAGSMPVVDSKRDKVIAKSTSN-SSTNSKVSD 558

Query: 1823 SSAAE--------------LPATGSVNASQGRSEKQL----------NELVAEEPPVGES 1930
             SA++               P     N + G   ++           NE   E   V  +
Sbjct: 559  VSASDPQLISSMPDNSVKNSPGAQQSNGNMGDGWQETLGPNSECLTSNEATPEVSSVSNT 618

Query: 1931 LRHSKDSISGGESTSQTRPDSEKISPSPAV-RPVLEDNIVLGVALEGSKRTLPIXXXXXX 2107
               S    +G    SQT+ D E+   SP + RP LE+NIVLGVALEGSKRTLPI      
Sbjct: 619  --ESVGERTGSPDISQTKQDIERSVASPLMTRPSLEENIVLGVALEGSKRTLPIEEEMDS 676

Query: 2108 XXXXTETKELATSRNGSVPTAITVDKEDQGGS 2203
                 E+KELA S+N   P+    DK+D   S
Sbjct: 677  STFPLESKELAASQNAG-PSPSGKDKKDSRDS 707


>XP_006338061.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            [Solanum tuberosum] XP_015167053.1 PREDICTED:
            mechanosensitive ion channel protein 3,
            chloroplastic-like [Solanum tuberosum]
          Length = 710

 Score =  341 bits (875), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 183/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            TQ+THWRIKTHLA+SHLDVNKIN IVADMRKVL+K+P +EQ++LHRRVFLDN+DPENQAL
Sbjct: 354  TQRTHWRIKTHLAISHLDVNKINNIVADMRKVLAKHPLVEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
            +IL+SCFVKT RFEEYL VKE +   +  ++  H
Sbjct: 414  KILISCFVKTPRFEEYLRVKEVILLDLLRVISHH 447



 Score =  225 bits (574), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 153/212 (72%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAA+ SLLLPRE  I    G   QSKG M +      +I LSS+  RQD WS+YLL++ H
Sbjct: 1   MAASVSLLLPREFPIHGKCGLSGQSKGTMSRSQTDSCSIFLSSHSTRQDPWSIYLLNTVH 60

Query: 409 GPLC--RVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
            PL     P R +V LCRSLLKPG   E  VL+ A+ +  RS+ ++ GSP++LQL+PA+G
Sbjct: 61  RPLFFHPSPTRCNVLLCRSLLKPGGGYESQVLETATLIWKRSLSTIHGSPLVLQLIPAIG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++VFA WG+ PL+HFGR +FL+++D+NW+ S+   +   YL+P+LLW GA LICRA+DP+
Sbjct: 121 VLVFAAWGLTPLMHFGRKLFLHESDSNWKQSSWHYVTTSYLKPVLLWTGAILICRAIDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           +LP+  SQAVKQR LNF+RSLSTV+A AYCLS
Sbjct: 181 VLPTVPSQAVKQRFLNFIRSLSTVMALAYCLS 212



 Score =  168 bits (426), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 153/269 (56%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ+  RE +VDD PF+D IF+R+R   N   LLIEPSYKI+ D+K+K
Sbjct: 445  SHHRARLATPIRTVQKTSREIDVDDVPFADSIFSRNRP--NRQVLLIEPSYKISSDDKAK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAHS-S 1828
             S RSVQS +EKD  ++AP       ++     T      + + SA  AN   K A S S
Sbjct: 503  ASARSVQSDEEKDQKVEAPSTSRGADDTNDKPSTLVEKEVVKASSASNANGDLKAATSPS 562

Query: 1829 AAELPATGSVNASQGRSEKQLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-IS 2005
              +    GS +  +  SEK     VA +PP G +   + +      S SQ + D+E+ IS
Sbjct: 563  DGKSLKQGSASPVKSNSEKNQIASVAGDPP-GLTSDTAIEKTDMASSASQAQQDTERPIS 621

Query: 2006 PSPAV-RPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVPT 2167
              P+V RP+LEDNIVLGVALEGSK TLPI               +E+KELA  RNG+  T
Sbjct: 622  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTPPSPSPTFFDSESKELAACRNGNSST 681

Query: 2168 AITVDKEDQ---GGSIPNTSSGDQREKER 2245
                DK D    G     ++S DQ+E+ER
Sbjct: 682  NSNKDKTDDKMPGAPSTQSTSNDQKERER 710


>XP_016460352.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Nicotiana tabacum]
          Length = 708

 Score =  342 bits (876), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 414  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 447



 Score =  214 bits (544), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 147/212 (69%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIHGKCGFTCQPKGTMSRIQTDSCSKFLSSHSSRQDAWSIYLLNSVR 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P R +V LCRSLLKPG   E   LK A+ +  RS+  + GSP++LQL+PA+G
Sbjct: 61  GPLFTHPSPTRCNVFLCRSLLKPGGGYESQFLKTAALIWKRSLNIIHGSPLVLQLVPAIG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           +IV A WG+ PL+ FGR  F +++ +NW+ S   ++ + YL+P+LLW GA LICRA+DP+
Sbjct: 121 VIVLAAWGLIPLMSFGRKFFFHESGSNWKQSGLHHVADSYLKPVLLWTGAILICRAVDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR   F+RSLSTV+A AYCLS
Sbjct: 181 ILPSAASQAVKQRFTTFIRSLSTVMALAYCLS 212



 Score =  178 bits (452), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 154/269 (57%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 445  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDRP--NRPFFLIEPSYRINNDDKTK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
            VS RSVQ  +EKD   +AP    A  ++   +        +   SA  AN   KVA  SS
Sbjct: 503  VSARSVQPDEEKDGKAEAPSTSQAADDTNGLLSKPTEKEVVKVSSASNANVDLKVATLSS 562

Query: 1829 AAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-I 2002
              + P  GS++  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ I
Sbjct: 563  DGKTPKQGSISPVKSNSEKNQVASATGEAPGLTSDINIEKTDVA--SAASQAQQDAERPI 620

Query: 2003 SPSPAVRPVLEDNIVLGVALEGSKRTLPI-------XXXXXXXXXXTETKELATSRNGSV 2161
            SP    RP+LEDNIVLGVALEGSK TLPI                 +E+KELA  RNG+ 
Sbjct: 621  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEEATPPSPSPSPTFSDSESKELAACRNGNS 680

Query: 2162 PTAITVDK-EDQGGSIPNTSSGDQREKER 2245
             T+   DK +DQ    P+  S  Q+E ER
Sbjct: 681  STSSNKDKMDDQMAEAPSAPS-VQKECER 708


>XP_009785624.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Nicotiana sylvestris]
          Length = 723

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 251  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 310

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 311  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 370

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 371  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 430

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 431  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 464



 Score =  219 bits (557), Expect(3) = 0.0
 Identities = 116/212 (54%), Positives = 150/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 18  MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSHSSRQDAWSIYLLNSVR 77

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P+R +V LCRSLLKPG   E   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 78  GPLFSHPPPIRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 137

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 138 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 197

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 198 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 229



 Score =  173 bits (439), Expect(3) = 0.0
 Identities = 117/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 462  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 519

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 520  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 578

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK- 1999
            S  + P  GS +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 579  SDGKTPKQGSSSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVAA--AASQAQQDAERP 636

Query: 2000 ISPSPAVRPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
            ISP    RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 637  ISPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 696

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 697  TSSNKDKMDDQMAEAPSAPNVQKEREK 723


>XP_009785640.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X3 [Nicotiana sylvestris] XP_009785647.1
            PREDICTED: mechanosensitive ion channel protein 3,
            chloroplastic-like isoform X3 [Nicotiana sylvestris]
            XP_009785652.1 PREDICTED: mechanosensitive ion channel
            protein 3, chloroplastic-like isoform X3 [Nicotiana
            sylvestris]
          Length = 706

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 414  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 447



 Score =  219 bits (557), Expect(3) = 0.0
 Identities = 116/212 (54%), Positives = 150/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSHSSRQDAWSIYLLNSVR 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P+R +V LCRSLLKPG   E   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 61  GPLFSHPPPIRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 121 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 181 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 212



 Score =  173 bits (439), Expect(3) = 0.0
 Identities = 117/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 445  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 503  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 561

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK- 1999
            S  + P  GS +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 562  SDGKTPKQGSSSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVAA--AASQAQQDAERP 619

Query: 2000 ISPSPAVRPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
            ISP    RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 620  ISPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 679

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 680  TSSNKDKMDDQMAEAPSAPNVQKEREK 706


>XP_010254532.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like
            [Nelumbo nucifera] XP_010254534.1 PREDICTED:
            mechanosensitive ion channel protein 2,
            chloroplastic-like [Nelumbo nucifera] XP_010254535.1
            PREDICTED: mechanosensitive ion channel protein 2,
            chloroplastic-like [Nelumbo nucifera]
          Length = 733

 Score =  347 bits (891), Expect(3) = 0.0
 Identities = 164/214 (76%), Positives = 185/214 (86%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+Y+AVWVAA SLFMELLGFSTQKW               REIFTNFLSS+M
Sbjct: 232  MGFSFAGKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVM 291

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF++N+WIQTKIEGYEVSGTVEHVGWWSPT+IRG+DREAVHIPNHKFTV VVRNL
Sbjct: 292  IHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNL 351

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QK+HWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQ+EQ++LHRRVFLDN+DPENQAL
Sbjct: 352  SQKSHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLDNVDPENQAL 411

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             IL+SCFVKTS FEEYLCVKEA+   +  ++  H
Sbjct: 412  LILISCFVKTSHFEEYLCVKEAILLDLLRVISHH 445



 Score =  216 bits (549), Expect(3) = 0.0
 Identities = 111/210 (52%), Positives = 154/210 (73%), Gaps = 1/210 (0%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKGM-DKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MA A SL L  EL + +N+    + K M  +  LH L++ LSS+ LRQD+W+  + SS +
Sbjct: 1   MAIAGSLQLSHELGLCKNHVHGNRLKMMMGRGRLHLLSVALSSHALRQDAWTQRVSSSIN 60

Query: 409 GPLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVGLI 588
            P+  +  R +V +C+S+L PG    IP+LK A+  L RS+ +L GSP++++L PAVG+I
Sbjct: 61  RPIHSLNSRYNVFMCQSVLLPGQGNGIPILKTATMALTRSLSALNGSPVVVKLAPAVGII 120

Query: 589 VFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPVIL 768
           +F +WG+ PL+   R+  L+++D++W+ SNT  ++  YLQPMLLW+GATLICRALDPV+L
Sbjct: 121 IFTIWGLGPLMRMTRNFLLHRSDSSWRKSNTHYVMTSYLQPMLLWVGATLICRALDPVVL 180

Query: 769 PSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           PS ASQAVKQRLL+FVRSLSTVLAFAYCLS
Sbjct: 181 PSEASQAVKQRLLHFVRSLSTVLAFAYCLS 210



 Score =  169 bits (428), Expect(3) = 0.0
 Identities = 111/291 (38%), Positives = 164/291 (56%), Gaps = 34/291 (11%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ++Y + ++++ PF+D IFTR+ +AAN PFLLIEP Y+IN D+K K
Sbjct: 443  SHHRARLATPIRTVQKIYSDKDMENVPFADTIFTRTGSAANRPFLLIEPPYRINNDDKMK 502

Query: 1652 VSPRSVQSSQEKDANI----------DAPLK--PDAVAESTLAVGT-----EKADGTLGS 1780
               R V++++E+DA +          DA +   P + +++ + VGT      KA+  + +
Sbjct: 503  AQGRPVRTNEEQDAKVAGTQTSDSKVDAKVGTGPSSDSKTDVKVGTTPMSDSKAETMVAA 562

Query: 1781 GSAGVANTMAKVAHSSAAELPATGSVNASQGRSEKQLNELVAEEPPVGESLRHSKDSISG 1960
             S   +    KVA  S+++  A   V A    SE ++    ++  P     + +K +  G
Sbjct: 563  TSIPDSKVDGKVAIVSSSDSKADPKVAAMSVSSESKMQSSASDGSPQTNPDKQAKKASPG 622

Query: 1961 GE-------------STSQTRPDSEK--ISPSPAVRPVLEDNIVLGVALEGSKRTLPIXX 2095
            G              +TS ++ D E+  ++P    RP LEDNIVLGVALEGSKRTLPI  
Sbjct: 623  GARQKSSKVANMPMATTSHSQQDGERPPVTPPQISRPALEDNIVLGVALEGSKRTLPIEE 682

Query: 2096 XXXXXXXXTETKELATSRNGSVPTAITVDKED-QGGSIPNTSSGDQREKER 2245
                     E KELA  RNG+  ++   DKE+ Q  + P  ++GDQR++ R
Sbjct: 683  GMDSSPTSAEAKELAACRNGNGSSSTGSDKEEGQIRAAPGATAGDQRDQGR 733


>XP_016481428.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X1 [Nicotiana tabacum]
          Length = 723

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 251  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 310

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 311  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 370

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 371  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 430

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 431  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 464



 Score =  218 bits (554), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 150/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 18  MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSHSSRQDAWSIYLLNSVR 77

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P+R +V LCRSLLKPG   +   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 78  GPLFSHPPPIRCNVLLCRSLLKPGGGYDSQFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 137

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 138 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 197

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 198 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 229



 Score =  173 bits (439), Expect(3) = 0.0
 Identities = 117/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 462  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 519

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 520  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 578

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK- 1999
            S  + P  GS +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 579  SDGKTPKQGSSSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVAA--AASQAQQDAERP 636

Query: 2000 ISPSPAVRPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
            ISP    RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 637  ISPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 696

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 697  TSSNKDKMDDQMAEAPSAPNVQKEREK 723


>XP_016481429.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X2 [Nicotiana tabacum] XP_016481430.1 PREDICTED:
            mechanosensitive ion channel protein 3,
            chloroplastic-like isoform X2 [Nicotiana tabacum]
          Length = 706

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 354  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 414  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 447



 Score =  218 bits (554), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 150/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSHSSRQDAWSIYLLNSVR 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P+R +V LCRSLLKPG   +   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 61  GPLFSHPPPIRCNVLLCRSLLKPGGGYDSQFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 121 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 181 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 212



 Score =  173 bits (439), Expect(3) = 0.0
 Identities = 117/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 445  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 503  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 561

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK- 1999
            S  + P  GS +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 562  SDGKTPKQGSSSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVAA--AASQAQQDAERP 619

Query: 2000 ISPSPAVRPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
            ISP    RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 620  ISPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 679

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 680  TSSNKDKMDDQMAEAPSAPNVQKEREK 706


>XP_002302344.2 hypothetical protein POPTR_0002s10610g [Populus trichocarpa]
            EEE81617.2 hypothetical protein POPTR_0002s10610g
            [Populus trichocarpa]
          Length = 671

 Score =  350 bits (897), Expect(3) = 0.0
 Identities = 168/214 (78%), Positives = 185/214 (86%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGF FAGKA+YTAVW+AA SLFMELLGFSTQKW               REIFTNFLSSIM
Sbjct: 229  MGFSFAGKAVYTAVWIAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIM 288

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LN+WIQTKIEGYEVSGTVEHVGWWSPT+IRGDDREAVHIPNHKF+V++VRNL
Sbjct: 289  IHATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFSVSIVRNL 348

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDV KIN IVADMRKVL+KNPQIEQ++LHRRVFLDNI+PENQAL
Sbjct: 349  SQKTHWRIKTHLAISHLDVKKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNINPENQAL 408

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             ILVSCFVKTS FEEYLCVKEAV   +  ++  H
Sbjct: 409  MILVSCFVKTSHFEEYLCVKEAVLLDLLRVISHH 442



 Score =  228 bits (580), Expect(3) = 0.0
 Identities = 116/209 (55%), Positives = 148/209 (70%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKGMDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAHG 411
           M  ASS+ L  EL++   +GC  Q     +  L  +N  LSS +LR D W L+ L+S  G
Sbjct: 1   MTRASSMKLSHELRVHNCHGCNNQYTSASRVQL--VNANLSSQFLRLDPWGLHFLNSTRG 58

Query: 412 PLCRVPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVGLIV 591
           P   VP R +V LCRS+L PG   EIPVLK A+    RS  +L GS ++L+L+PA+G+I 
Sbjct: 59  PPSPVPSRCNVFLCRSVLTPGGGNEIPVLKSAALAFTRSYDALRGSNLVLKLIPAIGIIT 118

Query: 592 FAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPVILP 771
           FA WG+ PL+  GR+IFL+K+DN+W+ S+T  ++  YLQP+LLW GATLICRALDPV+L 
Sbjct: 119 FAAWGLGPLIWLGRTIFLHKSDNSWKKSSTHYVVTSYLQPLLLWTGATLICRALDPVVLQ 178

Query: 772 SAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           S  SQAVKQRLLNFVRSLSTV+AFAYCLS
Sbjct: 179 SEVSQAVKQRLLNFVRSLSTVVAFAYCLS 207



 Score =  154 bits (388), Expect(3) = 0.0
 Identities = 109/258 (42%), Positives = 150/258 (58%), Gaps = 15/258 (5%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHH ARLATPIRTVQ++Y E ++++ PFSD IFTRS A ANHP LLIEPSYKIN ++K K
Sbjct: 440  SHHCARLATPIRTVQKIYSEADLENVPFSDSIFTRSGATANHPLLLIEPSYKINSEDKVK 499

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAHSSA 1831
             S RS+++++EKDA ++A L           V   KAD   GS           V  S  
Sbjct: 500  ASNRSLRANEEKDAKVEAAL-----------VSELKADAKAGS---------MPVVDSKR 539

Query: 1832 AELPATGSVNASQGRSEKQLNELVAEEP-----PVGESLRHSKDSISGGEST-----SQT 1981
             ++ A  + N+S   +  +++++ A +P     P G S+ +++   S GE T     SQ+
Sbjct: 540  DKVVAKSTSNSS---TNSKVSDVSASDPQLITTPEGSSVSNTE---SVGERTESPDISQS 593

Query: 1982 RPDSEKISPSPAV-RPVLEDNIVLGVALEGSKRTLP---IXXXXXXXXXXTETKELATSR 2149
            + D E+   SP + RP+LE+NIVLGVALEGSKRTLP   I           E+KELA S+
Sbjct: 594  KQDIERSVASPLMTRPLLEENIVLGVALEGSKRTLPIEEIEEEMDSSPFPLESKELAASQ 653

Query: 2150 NGSVPTAITVDKEDQGGS 2203
            N     ++  DK+D   S
Sbjct: 654  NAGQSPSVK-DKKDSRDS 670


>XP_018633013.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like
            isoform X2 [Nicotiana tomentosiformis]
          Length = 722

 Score =  342 bits (876), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 248  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 307

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 308  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 367

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 368  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 427

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 428  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 461



 Score =  208 bits (530), Expect(3) = 0.0
 Identities = 116/224 (51%), Positives = 148/224 (66%), Gaps = 3/224 (1%)
 Frame = +1

Query: 196 GSFFRFFCFP*NMAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQ 372
           G  F F      MAAA SLLLPREL I    G   Q KG M +      +  LSS+    
Sbjct: 5   GEAFFFLTVGQTMAAAVSLLLPRELGIHGKCGFTCQPKGTMSRIQTDSCSKFLSSH--SS 62

Query: 373 DSWSLYLLSSAHGPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEG 546
           D+WS+YLL+S  GPL     P R +V LCRSLLKPG   E   LK A+ +  RS+  + G
Sbjct: 63  DAWSIYLLNSVRGPLFTHPSPTRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNIIHG 122

Query: 547 SPIILQLLPAVGLIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWI 726
           SP++LQL+PA+G+IV A WG+ PL+ FGR  F +++ +NW+ S   ++ + YL+P+LLW 
Sbjct: 123 SPLVLQLVPAIGVIVLAAWGLIPLMSFGRKFFFHESGSNWKQSGLHHVADSYLKPVLLWT 182

Query: 727 GATLICRALDPVILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           GA LICRA+DP+ILPSAASQAVKQR   F+RSLSTV+A AYCLS
Sbjct: 183 GAILICRAVDPLILPSAASQAVKQRFTTFIRSLSTVMALAYCLS 226



 Score =  180 bits (457), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 155/269 (57%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 459  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDRP--NRPFFLIEPSYRINNDDKTK 516

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
            VS RSVQ  +EKD   +AP    A  ++   +        +   SA  AN   KVA  SS
Sbjct: 517  VSARSVQPDEEKDGKAEAPSTSQAADDTNGLLSKPTEKEVVKVSSASNANVDLKVATLSS 576

Query: 1829 AAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-I 2002
              + P  GS++  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ I
Sbjct: 577  DGKTPKQGSISPVKSNSEKNQVASATGEAPGLTSDINIEKTDVA--SAASQAQQDAERPI 634

Query: 2003 SPSPAVRPVLEDNIVLGVALEGSKRTLPI-------XXXXXXXXXXTETKELATSRNGSV 2161
            SP    RP+LEDNIVLGVALEGSK TLPI                 +E+KELA  RNG+ 
Sbjct: 635  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEEATPPSPSPSPTFSDSESKELAACRNGNS 694

Query: 2162 PTAITVDK-EDQGGSIPNTSSGDQREKER 2245
             T+   DK +DQ    P+  S  Q+E+ER
Sbjct: 695  STSSNKDKMDDQMAEAPSAPS-VQKERER 722


>XP_015073682.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X2 [Solanum pennellii]
          Length = 702

 Score =  340 bits (872), Expect(3) = 0.0
 Identities = 161/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 234  MGFEFAGKAIYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 293

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF++NDWIQTKI+GY+VSG VEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 294  IHATRPFVVNDWIQTKIQGYDVSGIVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 353

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            TQ+THWRIKTHLA+SHLDVNKIN IVADMRKVLSK+P +EQ++LHRRVFLDN+DPENQAL
Sbjct: 354  TQRTHWRIKTHLAISHLDVNKINNIVADMRKVLSKHPMVEQQRLHRRVFLDNVDPENQAL 413

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
            +IL+SCFVKT RFEEYL VKE +   +  ++  H
Sbjct: 414  KILISCFVKTPRFEEYLRVKEVILLDLLRVISHH 447



 Score =  221 bits (562), Expect(3) = 0.0
 Identities = 114/212 (53%), Positives = 152/212 (71%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MA + SLLLPREL I    G   QSKG M +      +I LSS+  RQD WS+YLL++ H
Sbjct: 1   MAVSVSLLLPRELGIHGKCGLSGQSKGTMSRSQTDSCSIFLSSHSTRQDPWSIYLLNTVH 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
            PL     P R +V LCRSLLKPG   E  VL+ A+ +  RS+ ++ GSP++LQL+PA+G
Sbjct: 61  RPLFSHPSPSRCNVLLCRSLLKPGGGYESQVLETATLIWKRSLSTIHGSPLVLQLIPAIG 120

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++VFA WG+ PL+ FGR +FL+++D+NW+ S+   +   YL+P+LLW GA LICRA+DP+
Sbjct: 121 VLVFAAWGLTPLMRFGRKLFLHESDSNWKQSSWHYVTASYLKPVLLWTGAILICRAIDPL 180

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           +LP+  SQAVKQR LNF+RSLSTV+A AYCLS
Sbjct: 181 VLPTVPSQAVKQRFLNFIRSLSTVMALAYCLS 212



 Score =  166 bits (420), Expect(3) = 0.0
 Identities = 115/273 (42%), Positives = 152/273 (55%), Gaps = 16/273 (5%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATPIRTVQ+  RE + DD PF+D IF+R+R   N   LLIEPSYKI+ D+K+K
Sbjct: 445  SHHRARLATPIRTVQKTSREIDADDVPFADSIFSRNRP--NRQVLLIEPSYKISSDDKAK 502

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAHSSA 1831
             S RSVQ  +EKD  ++AP+             +  AD T G  S  V   + K + +S 
Sbjct: 503  ASARSVQPDEEKDQKVEAPIT------------SRTADDTNGKPSTLVEKEVDKASSASN 550

Query: 1832 AELPATGSVNASQGRSEKQLN----------ELVAEEPPVGESLRHSKDSISGGESTSQT 1981
            A      + + S G+S KQ +            VA +PP G +   + +        SQ 
Sbjct: 551  ANGDLKAATSPSDGKSLKQGSASPVKSNSEKNQVAGDPP-GLTSDTNIEKTDAASYASQA 609

Query: 1982 RPDSEK-ISPSPAV-RPVLEDNIVLGVALEGSKRTLPIXXXXXXXXXXTETKELATSRNG 2155
            + D+E+ IS  P+V RP+LEDNIVLGVALEGSK TLPI          +E+KELA  RNG
Sbjct: 610  QQDTERPISTPPSVGRPMLEDNIVLGVALEGSKLTLPIEEDETTPPSPSESKELAACRNG 669

Query: 2156 SVPTAITVDKEDQ---GGSIPNTSSGDQREKER 2245
            +  T    DK D    G     ++S +Q+E+ER
Sbjct: 670  NSSTNSNKDKTDDKMPGAPSTQSTSNEQKERER 702


>XP_016460353.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like
            isoform X2 [Nicotiana tabacum]
          Length = 706

 Score =  342 bits (876), Expect(3) = 0.0
 Identities = 163/214 (76%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVAA SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 232  MGFEFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 291

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 292  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 351

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 352  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 411

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 412  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 445



 Score =  206 bits (524), Expect(3) = 0.0
 Identities = 113/212 (53%), Positives = 145/212 (68%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+    D+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIHGKCGFTCQPKGTMSRIQTDSCSKFLSSH--SSDAWSIYLLNSVR 58

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P R +V LCRSLLKPG   E   LK A+ +  RS+  + GSP++LQL+PA+G
Sbjct: 59  GPLFTHPSPTRCNVFLCRSLLKPGGGYESQFLKTAALIWKRSLNIIHGSPLVLQLVPAIG 118

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           +IV A WG+ PL+ FGR  F +++ +NW+ S   ++ + YL+P+LLW GA LICRA+DP+
Sbjct: 119 VIVLAAWGLIPLMSFGRKFFFHESGSNWKQSGLHHVADSYLKPVLLWTGAILICRAVDPL 178

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR   F+RSLSTV+A AYCLS
Sbjct: 179 ILPSAASQAVKQRFTTFIRSLSTVMALAYCLS 210



 Score =  178 bits (452), Expect(3) = 0.0
 Identities = 119/269 (44%), Positives = 154/269 (57%), Gaps = 12/269 (4%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 443  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDRP--NRPFFLIEPSYRINNDDKTK 500

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKADGTLGSGSAGVANTMAKVAH-SS 1828
            VS RSVQ  +EKD   +AP    A  ++   +        +   SA  AN   KVA  SS
Sbjct: 501  VSARSVQPDEEKDGKAEAPSTSQAADDTNGLLSKPTEKEVVKVSSASNANVDLKVATLSS 560

Query: 1829 AAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK-I 2002
              + P  GS++  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ I
Sbjct: 561  DGKTPKQGSISPVKSNSEKNQVASATGEAPGLTSDINIEKTDVA--SAASQAQQDAERPI 618

Query: 2003 SPSPAVRPVLEDNIVLGVALEGSKRTLPI-------XXXXXXXXXXTETKELATSRNGSV 2161
            SP    RP+LEDNIVLGVALEGSK TLPI                 +E+KELA  RNG+ 
Sbjct: 619  SPPSVGRPMLEDNIVLGVALEGSKLTLPIEEEATPPSPSPSPTFSDSESKELAACRNGNS 678

Query: 2162 PTAITVDK-EDQGGSIPNTSSGDQREKER 2245
             T+   DK +DQ    P+  S  Q+E ER
Sbjct: 679  STSSNKDKMDDQMAEAPSAPS-VQKECER 706


>XP_009785633.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            isoform X2 [Nicotiana sylvestris]
          Length = 721

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 249  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 308

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 309  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 368

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 369  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 428

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 429  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 462



 Score =  211 bits (537), Expect(3) = 0.0
 Identities = 114/212 (53%), Positives = 148/212 (69%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+    D+WS+YLL+S  
Sbjct: 18  MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSH--SSDAWSIYLLNSVR 75

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P+R +V LCRSLLKPG   E   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 76  GPLFSHPPPIRCNVLLCRSLLKPGGGYESQFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 135

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 136 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 195

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 196 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 227



 Score =  173 bits (439), Expect(3) = 0.0
 Identities = 117/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 460  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 517

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 518  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 576

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEK- 1999
            S  + P  GS +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 577  SDGKTPKQGSSSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVAA--AASQAQQDAERP 634

Query: 2000 ISPSPAVRPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
            ISP    RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 635  ISPPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 694

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 695  TSSNKDKMDDQMAEAPSAPNVQKEREK 721


>XP_019266973.1 PREDICTED: mechanosensitive ion channel protein 3, chloroplastic-like
            [Nicotiana attenuata] OIT34708.1 mechanosensitive ion
            channel protein 2, chloroplastic [Nicotiana attenuata]
          Length = 705

 Score =  340 bits (873), Expect(3) = 0.0
 Identities = 162/214 (75%), Positives = 182/214 (85%)
 Frame = +3

Query: 861  MGFEFAGKALYTAVWVAAFSLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 1040
            MGFEFAGKA+YTAVWVA+ SLFMELLGFSTQKW               REI TNFLSSIM
Sbjct: 233  MGFEFAGKAVYTAVWVASVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREILTNFLSSIM 292

Query: 1041 IHTTRPFILNDWIQTKIEGYEVSGTVEHVGWWSPTVIRGDDREAVHIPNHKFTVTVVRNL 1220
            IH TRPF+LNDWIQTKI+GY+VSGTVEHVGWWSPTVIRGDDREA+HIPNHKF+V +VRNL
Sbjct: 293  IHATRPFVLNDWIQTKIQGYDVSGTVEHVGWWSPTVIRGDDREAIHIPNHKFSVNIVRNL 352

Query: 1221 TQKTHWRIKTHLAVSHLDVNKINVIVADMRKVLSKNPQIEQKKLHRRVFLDNIDPENQAL 1400
            +QKTHWRIKTHLA+SHLDVNKIN IVADMRKVL+KNPQIEQ++LHRRVFLDN+DPENQAL
Sbjct: 353  SQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQIEQQRLHRRVFLDNVDPENQAL 412

Query: 1401 QILVSCFVKTSRFEEYLCVKEAVXSVITGLVLPH 1502
             I++SCFVKTS FEEY  VKE +   +  ++  H
Sbjct: 413  MIMISCFVKTSYFEEYSRVKEVILLDLLRVISHH 446



 Score =  213 bits (541), Expect(3) = 0.0
 Identities = 115/212 (54%), Positives = 149/212 (70%), Gaps = 3/212 (1%)
 Frame = +1

Query: 232 MAAASSLLLPRELKIDRNNGCFFQSKG-MDKCHLHPLNIPLSSNYLRQDSWSLYLLSSAH 408
           MAAA SLLLPREL I    G   Q KG M +      +  LSS+  RQD+WS+YLL+S  
Sbjct: 1   MAAAVSLLLPRELGIQGKCGFTCQPKGTMSRSQTDSCSKFLSSHSSRQDAWSIYLLNSVR 60

Query: 409 GPLCR--VPVRLSVCLCRSLLKPGVVQEIPVLKGASTMLMRSVKSLEGSPIILQLLPAVG 582
           GPL     P R +V LCR+LLKPG   E   LK A+ +  RS+ ++ GSP++LQL+PAVG
Sbjct: 61  GPLFSHPPPTRCNVLLCRALLKPGGGYE-QFLKTAALIWKRSLNTIHGSPLVLQLVPAVG 119

Query: 583 LIVFAVWGVQPLVHFGRSIFLNKNDNNWQSSNTFNILNKYLQPMLLWIGATLICRALDPV 762
           ++V A WG+ PL+ FGR  F +++++NW+ S    + + YL+P+LLW GA LICRA+DP+
Sbjct: 120 VLVLAAWGLIPLMRFGRKFFFHESESNWKQSGLHYVADSYLKPVLLWTGAILICRAVDPL 179

Query: 763 ILPSAASQAVKQRLLNFVRSLSTVLAFAYCLS 858
           ILPSAASQAVKQR L F+RSLSTV+A AYCLS
Sbjct: 180 ILPSAASQAVKQRFLTFIRSLSTVMALAYCLS 211



 Score =  171 bits (432), Expect(3) = 0.0
 Identities = 115/267 (43%), Positives = 155/267 (58%), Gaps = 10/267 (3%)
 Frame = +2

Query: 1475 SHHRARLATPIRTVQRVYREPEVDDAPFSD-IFTRSRAAANHPFLLIEPSYKINGDEKSK 1651
            SHHRARLATP+RTVQ+ YREP+ DD PF+D IF+R R   N PF LIEPSY+IN D+K+K
Sbjct: 444  SHHRARLATPLRTVQKTYREPDADDVPFADSIFSRDR--PNRPFFLIEPSYRINNDDKTK 501

Query: 1652 VSPRSVQSSQEKDANIDAPLKPDAVAESTLAVGTEKAD-GTLGSGSAGVANTMAKVAH-S 1825
            VS  SVQ  +EKD   +AP    A A+ T  + ++ A+   +   SA  AN   KVA  S
Sbjct: 502  VSAGSVQPDEEKDGKAEAPSTSQA-ADDTNGLPSKPAEKEVVKVSSASNANVDLKVATLS 560

Query: 1826 SAAELPATGSVNASQGRSEK-QLNELVAEEPPVGESLRHSKDSISGGESTSQTRPDSEKI 2002
            S  + P  G  +  +  SEK Q+     E P +   +   K  ++   + SQ + D+E+ 
Sbjct: 561  SDGKTPKQGLTSPVKSNSEKNQVASATGEPPGLTSDINIEKTDVA--SAASQAQQDAERP 618

Query: 2003 SPSPAV-RPVLEDNIVLGVALEGSKRTLPI-----XXXXXXXXXXTETKELATSRNGSVP 2164
              +P+V RP+LEDNIVLGVALEGSK TLPI               +E KELA  RNG+  
Sbjct: 619  ISTPSVGRPMLEDNIVLGVALEGSKLTLPIEEETTSPSPSPAFSDSEPKELAACRNGNSS 678

Query: 2165 TAITVDKEDQGGSIPNTSSGDQREKER 2245
            T+   DK D   +   ++   Q+E+E+
Sbjct: 679  TSSNKDKMDDQMAEAPSAPNVQKEREK 705