BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00031083
         (435 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI27756.3| unnamed protein product [Vitis vinifera]              144   1e-32
ref|XP_002277473.1| PREDICTED: pectinesterase 3 [Vitis vinifera]      144   1e-32
gb|ABG46324.1| putative pectin methylesterase [Picea abies]           116   2e-24
ref|NP_189913.3| pectinesterase 32 [Arabidopsis thaliana] gi|753...   115   5e-24
gb|AAL38790.1| putative pectinesterase [Arabidopsis thaliana] gi...   114   8e-24

>emb|CBI27756.3| unnamed protein product [Vitis vinifera]
          Length = 537

 Score =  144 bits (362), Expect = 1e-32
 Identities = 73/140 (52%), Positives = 101/140 (72%), Gaps = 1/140 (0%)
 Frame = -2

Query: 434 ISNCLAIVKYTENQHMK-RAIINPRMLIARNRVEEFQDWVSNVDRKMMEIAAVMKPNVTV 258
           ISNCLAI+KY E   ++ R I+N  M       +EF  W++ +DRK++E+   ++P++ V
Sbjct: 216 ISNCLAIIKYMETIALRDRKIMNTTM-----PRDEFPAWMTAIDRKLIEMVPKIRPDIVV 270

Query: 257 ASDGCGNFTNITNAIEMAPNFSKIRYIIHVKAGNYCENVVVPRGKINIMLIGDGMGSTII 78
           ASDG G+F+ I  AI  APN S  R++I +KAG Y ENV +PR K+NIML+G+GM ST+I
Sbjct: 271 ASDGSGHFSTIGEAISTAPNKSSNRFVIKIKAGVYKENVEIPREKVNIMLVGEGMNSTVI 330

Query: 77  TGSRSFADGFSTFASATLSM 18
           TGS+SF DGFSTF SATL++
Sbjct: 331 TGSKSFVDGFSTFTSATLTV 350


>ref|XP_002277473.1| PREDICTED: pectinesterase 3 [Vitis vinifera]
          Length = 582

 Score =  144 bits (362), Expect = 1e-32
 Identities = 73/140 (52%), Positives = 101/140 (72%), Gaps = 1/140 (0%)
 Frame = -2

Query: 434 ISNCLAIVKYTENQHMK-RAIINPRMLIARNRVEEFQDWVSNVDRKMMEIAAVMKPNVTV 258
           ISNCLAI+KY E   ++ R I+N  M       +EF  W++ +DRK++E+   ++P++ V
Sbjct: 219 ISNCLAIIKYMETIALRDRKIMNTTM-----PRDEFPAWMTAIDRKLIEMVPKIRPDIVV 273

Query: 257 ASDGCGNFTNITNAIEMAPNFSKIRYIIHVKAGNYCENVVVPRGKINIMLIGDGMGSTII 78
           ASDG G+F+ I  AI  APN S  R++I +KAG Y ENV +PR K+NIML+G+GM ST+I
Sbjct: 274 ASDGSGHFSTIGEAISTAPNKSSNRFVIKIKAGVYKENVEIPREKVNIMLVGEGMNSTVI 333

Query: 77  TGSRSFADGFSTFASATLSM 18
           TGS+SF DGFSTF SATL++
Sbjct: 334 TGSKSFVDGFSTFTSATLTV 353


>gb|ABG46324.1| putative pectin methylesterase [Picea abies]
          Length = 393

 Score =  116 bits (290), Expect = 2e-24
 Identities = 64/152 (42%), Positives = 96/152 (63%), Gaps = 13/152 (8%)
 Frame = -2

Query: 434 ISNCLAIVKY--TENQHMKRAII---NPRMLIARNRV-------EEFQDWVSNVDRKMME 291
           +SN LA+VK    E  ++ R  +   N R+L  ++         + F  W+S  DR++++
Sbjct: 177 VSNSLAMVKNISAEASNLARRTVSNQNRRLLSDQSDPNFMPMDSDGFPSWMSAGDRRLLQ 236

Query: 290 IAA-VMKPNVTVASDGCGNFTNITNAIEMAPNFSKIRYIIHVKAGNYCENVVVPRGKINI 114
            +   +KPN  VA DG GN+TNIT A+E AP  SK RY+IH+KAG Y ENV + + K N+
Sbjct: 237 TSTGTVKPNAVVAKDGSGNYTNITEAVEAAPEKSKTRYVIHIKAGVYAENVELHKKKTNL 296

Query: 113 MLIGDGMGSTIITGSRSFADGFSTFASATLSM 18
           M IGDGM  T++TG+R+  + F+TF SAT+++
Sbjct: 297 MFIGDGMDVTVVTGNRNVKENFTTFRSATVAV 328


>ref|NP_189913.3| pectinesterase 32 [Arabidopsis thaliana]
           gi|75335617|sp|Q9LXK7.1|PME32_ARATH RecName:
           Full=Probable pectinesterase/pectinesterase inhibitor
           32; Includes: RecName: Full=Pectinesterase inhibitor 32;
           AltName: Full=Pectin methylesterase inhibitor 32;
           Includes: RecName: Full=Pectinesterase 32; Short=PE 32;
           AltName: Full=Pectin methylesterase 32; Short=AtPME32;
           Flags: Precursor gi|7649367|emb|CAB89048.1|
           pectinesterase-like protein [Arabidopsis thaliana]
           gi|17979141|gb|AAL49828.1| putative pectinesterase
           [Arabidopsis thaliana] gi|20465719|gb|AAM20328.1|
           putative pectinesterase [Arabidopsis thaliana]
           gi|332644260|gb|AEE77781.1| pectinesterase 32
           [Arabidopsis thaliana]
          Length = 527

 Score =  115 bits (287), Expect = 5e-24
 Identities = 57/139 (41%), Positives = 88/139 (63%)
 Frame = -2

Query: 434 ISNCLAIVKYTENQHMKRAIINPRMLIARNRVEEFQDWVSNVDRKMMEIAAVMKPNVTVA 255
           + N L +V    ++   + I    M  A +   +F  WV   DRK+++   +   +  VA
Sbjct: 159 VRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVA 218

Query: 254 SDGCGNFTNITNAIEMAPNFSKIRYIIHVKAGNYCENVVVPRGKINIMLIGDGMGSTIIT 75
           +DG GNFT I++A+  AP++S  RY+IHVK G Y ENV + + K NIM++GDG+ +T+IT
Sbjct: 219 ADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVIT 278

Query: 74  GSRSFADGFSTFASATLSM 18
           G+RSF DG++TF SAT ++
Sbjct: 279 GNRSFIDGWTTFRSATFAV 297


>gb|AAL38790.1| putative pectinesterase [Arabidopsis thaliana]
           gi|20466039|gb|AAM20354.1| putative pectinesterase
           [Arabidopsis thaliana]
          Length = 305

 Score =  114 bits (285), Expect = 8e-24
 Identities = 57/136 (41%), Positives = 86/136 (63%)
 Frame = -2

Query: 434 ISNCLAIVKYTENQHMKRAIINPRMLIARNRVEEFQDWVSNVDRKMMEIAAVMKPNVTVA 255
           + N L +V    ++   + I    M  A +   +F  WV   DRK+++   +   +  VA
Sbjct: 159 VRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVA 218

Query: 254 SDGCGNFTNITNAIEMAPNFSKIRYIIHVKAGNYCENVVVPRGKINIMLIGDGMGSTIIT 75
           +DG GNFT I++A+  AP++S  RY+IHVK G Y ENV + + K NIM++GDG+ +T+IT
Sbjct: 219 ADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVIT 278

Query: 74  GSRSFADGFSTFASAT 27
           G+RSF DG++TF SAT
Sbjct: 279 GNRSFIDGWTTFRSAT 294