BLASTX nr result
ID: Lithospermum22_contig00027696
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00027696
(538 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI24150.3| unnamed protein product [Vitis vinifera] 184 6e-53
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop... 184 6e-53
ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa] g... 184 7e-53
gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima] 183 1e-52
gb|AEP82778.1| ent-kaurene synthase [Solanum lycopersicum] 175 9e-52
>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
Length = 826
Score = 184 bits (468), Expect(2) = 6e-53
Identities = 87/132 (65%), Positives = 108/132 (81%)
Frame = +2
Query: 26 RWVTDNTLDKLKFARQKSAYCYFSAAATLVSPELSDARMSWAKNCVLTTVIDDFSDVGGT 205
RW+ +N LDKLKFARQK AYCYFSAAAT+ SPE SDAR+SWAKN VLTTV+DDF D+GG+
Sbjct: 524 RWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGS 583
Query: 206 IGEMKNLIELVERWDVSNRSDCCSEQVEIMFSALKHTIFELGEKTLAWQSQDETNHIIQI 385
E+ NLI+LVE+WD+ DCCSEQVEI+FSAL TI E+G K AWQ+++ T+HII I
Sbjct: 584 EEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQARNVTSHIIDI 643
Query: 386 WLELLKSGLKRS 421
WL+LL+S L+ +
Sbjct: 644 WLKLLRSMLQEA 655
Score = 48.1 bits (113), Expect(2) = 6e-53
Identities = 22/42 (52%), Positives = 29/42 (69%), Gaps = 3/42 (7%)
Frame = +3
Query: 414 KEAEWARSMSVPTLDEYISNI---LCFRAIVLPALYFIGPKL 530
+EA+W + S PT+DEY++N IVLPALYF+GPKL
Sbjct: 653 QEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPKL 694
>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
vinifera]
Length = 793
Score = 184 bits (468), Expect(2) = 6e-53
Identities = 87/132 (65%), Positives = 108/132 (81%)
Frame = +2
Query: 26 RWVTDNTLDKLKFARQKSAYCYFSAAATLVSPELSDARMSWAKNCVLTTVIDDFSDVGGT 205
RW+ +N LDKLKFARQK AYCYFSAAAT+ SPE SDAR+SWAKN VLTTV+DDF D+GG+
Sbjct: 491 RWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGS 550
Query: 206 IGEMKNLIELVERWDVSNRSDCCSEQVEIMFSALKHTIFELGEKTLAWQSQDETNHIIQI 385
E+ NLI+LVE+WD+ DCCSEQVEI+FSAL TI E+G K AWQ+++ T+HII I
Sbjct: 551 EEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQARNVTSHIIDI 610
Query: 386 WLELLKSGLKRS 421
WL+LL+S L+ +
Sbjct: 611 WLKLLRSMLQEA 622
Score = 48.1 bits (113), Expect(2) = 6e-53
Identities = 22/42 (52%), Positives = 29/42 (69%), Gaps = 3/42 (7%)
Frame = +3
Query: 414 KEAEWARSMSVPTLDEYISNI---LCFRAIVLPALYFIGPKL 530
+EA+W + S PT+DEY++N IVLPALYF+GPKL
Sbjct: 620 QEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPKL 661
>ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa]
gi|222851106|gb|EEE88653.1| ent-kaurene synthase
[Populus trichocarpa]
Length = 743
Score = 184 bits (468), Expect(2) = 7e-53
Identities = 89/137 (64%), Positives = 111/137 (81%)
Frame = +2
Query: 2 EFAYLESNRWVTDNTLDKLKFARQKSAYCYFSAAATLVSPELSDARMSWAKNCVLTTVID 181
EF ++E RWV + LDKLKFARQK AYCYFSAAATL +PELSDARMSWAKN VLTTV+D
Sbjct: 435 EFKHIE--RWVVERRLDKLKFARQKEAYCYFSAAATLFAPELSDARMSWAKNGVLTTVVD 492
Query: 182 DFSDVGGTIGEMKNLIELVERWDVSNRSDCCSEQVEIMFSALKHTIFELGEKTLAWQSQD 361
DF DVGG+ E+ NLIEL+ERWDV+ +D CSE+VEI++SA+ TI E+G+K+ WQ +D
Sbjct: 493 DFFDVGGSEEELVNLIELIERWDVNGSADFCSEEVEIIYSAIHSTISEIGDKSFGWQGRD 552
Query: 362 ETNHIIQIWLELLKSGL 412
+ +I+IWL+LLKS L
Sbjct: 553 VKSQVIKIWLDLLKSML 569
Score = 48.1 bits (113), Expect(2) = 7e-53
Identities = 23/41 (56%), Positives = 29/41 (70%), Gaps = 3/41 (7%)
Frame = +3
Query: 417 EAEWARSMSVPTLDEYISNI---LCFRAIVLPALYFIGPKL 530
EA+W+ + SVPTLDEY++ IVLPALYF+GPKL
Sbjct: 571 EAQWSSNKSVPTLDEYMTTAHVSFALGPIVLPALYFVGPKL 611
>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
Length = 784
Score = 183 bits (464), Expect(2) = 1e-52
Identities = 89/132 (67%), Positives = 106/132 (80%)
Frame = +2
Query: 26 RWVTDNTLDKLKFARQKSAYCYFSAAATLVSPELSDARMSWAKNCVLTTVIDDFSDVGGT 205
RW+T+N LDKLKFARQK AYCYFSAAATL SPELSDAR+SWAKN VLTTV+DDF DVGG+
Sbjct: 482 RWITENRLDKLKFARQKLAYCYFSAAATLFSPELSDARISWAKNGVLTTVVDDFFDVGGS 541
Query: 206 IGEMKNLIELVERWDVSNRSDCCSEQVEIMFSALKHTIFELGEKTLAWQSQDETNHIIQI 385
+ E+ NLI+LVE+WDV +DCCSE VEI+FSAL TI + +K L Q ++ +HII I
Sbjct: 542 VEELVNLIQLVEKWDVDVSTDCCSENVEIIFSALHSTICDFADKGLTLQGRNVISHIIDI 601
Query: 386 WLELLKSGLKRS 421
WL LLKS LK +
Sbjct: 602 WLNLLKSMLKEA 613
Score = 48.9 bits (115), Expect(2) = 1e-52
Identities = 24/42 (57%), Positives = 29/42 (69%), Gaps = 3/42 (7%)
Frame = +3
Query: 414 KEAEWARSMSVPTLDEYISN---ILCFRAIVLPALYFIGPKL 530
KEAEW R SVP++DEY++N IVLPALY +GPKL
Sbjct: 611 KEAEWLRDKSVPSMDEYMTNGYVSFALGPIVLPALYCVGPKL 652
>gb|AEP82778.1| ent-kaurene synthase [Solanum lycopersicum]
Length = 820
Score = 175 bits (443), Expect(2) = 9e-52
Identities = 83/132 (62%), Positives = 102/132 (77%)
Frame = +2
Query: 26 RWVTDNTLDKLKFARQKSAYCYFSAAATLVSPELSDARMSWAKNCVLTTVIDDFSDVGGT 205
RW+T N LDKLKF R++SAYCYFSAAAT+ PELSDARMSWAKN VLTTVIDDF DVGG+
Sbjct: 508 RWLTQNRLDKLKFVRERSAYCYFSAAATIFQPELSDARMSWAKNGVLTTVIDDFFDVGGS 567
Query: 206 IGEMKNLIELVERWDVSNRSDCCSEQVEIMFSALKHTIFELGEKTLAWQSQDETNHIIQI 385
+ E+ NLI L ++WDV +DCCSE+V I+FSAL TI E+G+K WQ++ T HI I
Sbjct: 568 MEELNNLILLFKKWDVDVSTDCCSERVGIIFSALHSTISEIGDKASKWQARSVTRHITDI 627
Query: 386 WLELLKSGLKRS 421
WL LL + L+ +
Sbjct: 628 WLNLLNAMLREA 639
Score = 53.9 bits (128), Expect(2) = 9e-52
Identities = 25/43 (58%), Positives = 32/43 (74%), Gaps = 3/43 (6%)
Frame = +3
Query: 414 KEAEWARSMSVPTLDEYISN---ILCFRAIVLPALYFIGPKLP 533
+EAEWA+ MSVP+LD+Y++N I LPALYF+GPKLP
Sbjct: 637 REAEWAKDMSVPSLDKYMANGYVSFALGPIFLPALYFVGPKLP 679