BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00022724
         (1383 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAC31698.1| delta-12 fatty acid desaturase [Borago officinalis]    723   0.0  
gb|ADK47520.1| microsomal oleic acid desaturase [Wrightia tincto...   689   0.0  
gb|AFK31315.1| fatty acid desaturase [Camellia oleifera]              686   0.0  
gb|AAW63041.1| microsomal delta-12 oleate desaturase [Olea europ...   682   0.0  
gb|AAN87573.1| delta 12 oleic acid desaturase FAD2 [Vernicia for...   675   0.0  

>gb|AAC31698.1| delta-12 fatty acid desaturase [Borago officinalis]
          Length = 383

 Score =  723 bits (1867), Expect = 0.0
 Identities = 334/383 (87%), Positives = 356/383 (92%), Gaps = 1/383 (0%)
 Frame = -2

Query: 1319 MGAGGRMSVPSKGKKSKSDVFQRVPCEKPPFTVGDIKKAIPPHCFQRSVLHSFSYVVYDL 1140
            MG GGRM VP+KGKKSKSDVFQRVP EKPPFTVGD+KK IPPHCFQRSVLHSFSYVVYDL
Sbjct: 1    MGGGGRMPVPTKGKKSKSDVFQRVPSEKPPFTVGDLKKVIPPHCFQRSVLHSFSYVVYDL 60

Query: 1139 TIAFLFYYAASNYIHLLPCPLSYLAWPIYWFCQGCVLTGVWVIAHECGHHAFSDYQWLDD 960
             IA LF+Y AS YIHL P PLSY+AWP+YWFCQG VLTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61   VIAALFFYTASRYIHLQPHPLSYVAWPLYWFCQGSVLTGVWVIAHECGHHAFSDYQWLDD 120

Query: 959  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKRKSGISWFLKYLNNPPGRIL 780
            TVGL+LHS+LLVPYFSWKYSHRRHHSNTGSLERDEVFVPK++SGISW  +YLNNPPGR+L
Sbjct: 121  TVGLLLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKRSGISWSSEYLNNPPGRVL 180

Query: 779  ILLVQLILGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIFISDAGITAMTYLLY 600
            +LLVQL LGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQI+ISDAGI A+ Y LY
Sbjct: 181  VLLVQLTLGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIYISDAGIVAVMYGLY 240

Query: 599  RLVAAKGLSWVFCYYGAPLLIVNGYLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 420
            RLVAAKG++WV CYYG PLL+VNG+LVLITYLQHT P+LPHYDSSEWDWL+GALATVDRD
Sbjct: 241  RLVAAKGVAWVVCYYGVPLLVVNGFLVLITYLQHTQPSLPHYDSSEWDWLKGALATVDRD 300

Query: 419  YGFLNKVFHNITNTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVFKAMYREVK 240
            YGFLNKV HNIT+THVAHHLFSTMPHYHAMEATKAIKPILG+YYQ D TPVFKAMYREVK
Sbjct: 301  YGFLNKVLHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQCDRTPVFKAMYREVK 360

Query: 239  ECIYVEPDETDQKKGVFWY-NKL 174
            ECIYVE DE D KKGVFWY NKL
Sbjct: 361  ECIYVEADEGDNKKGVFWYKNKL 383


>gb|ADK47520.1| microsomal oleic acid desaturase [Wrightia tinctoria]
          Length = 383

 Score =  689 bits (1779), Expect = 0.0
 Identities = 314/383 (81%), Positives = 348/383 (90%), Gaps = 1/383 (0%)
 Frame = -2

Query: 1319 MGAGGRMSVPSKGKKSKSDVFQRVPCEKPPFTVGDIKKAIPPHCFQRSVLHSFSYVVYDL 1140
            MGAGGRM++P +GKK+KS V  RVP  KPPF++G+IKKAIPPHCFQRSVL SFSYV++DL
Sbjct: 1    MGAGGRMNIPHEGKKTKSGVPGRVPYSKPPFSLGEIKKAIPPHCFQRSVLTSFSYVIFDL 60

Query: 1139 TIAFLFYYAASNYIHLLPCPLSYLAWPIYWFCQGCVLTGVWVIAHECGHHAFSDYQWLDD 960
             IA LFYY A+NYI LLP P +YLAWP+YW CQGC+LTGVWVIAHECGHHAFSDYQWLDD
Sbjct: 61   AIASLFYYIATNYIPLLPHPFAYLAWPLYWICQGCILTGVWVIAHECGHHAFSDYQWLDD 120

Query: 959  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKRKSGISWFLKYLNNPPGRIL 780
            TVGL+LHS+LLVPYFSWKYSHRRHHSNTGSLERDEVFVPK KS + WF KYLNNPPGR+L
Sbjct: 121  TVGLVLHSALLVPYFSWKYSHRRHHSNTGSLERDEVFVPKVKSNVRWFGKYLNNPPGRVL 180

Query: 779  ILLVQLILGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIFISDAGITAMTYLLY 600
             L+ QL LGWPLYLMFNVSGRPYDRFACH+DP  PIY+DRERLQI+ISDAG+ A+ Y LY
Sbjct: 181  TLVFQLTLGWPLYLMFNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVFAVAYGLY 240

Query: 599  RLVAAKGLSWVFCYYGAPLLIVNGYLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 420
             LVAAKGL+WVFC YG PLL+VNG+LVLITYLQHTHP+LPHYDSSEWDWLRGALATVDRD
Sbjct: 241  LLVAAKGLAWVFCVYGGPLLVVNGFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 419  YGFLNKVFHNITNTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVFKAMYREVK 240
            YG LNKVFH+IT+THVAHHLFSTMPHYHAMEATKAIKPIL EYYQFDGTP+F+AM+RE K
Sbjct: 301  YGILNKVFHHITDTHVAHHLFSTMPHYHAMEATKAIKPILEEYYQFDGTPIFQAMWREAK 360

Query: 239  ECIYVEPDETDQKKGVFWY-NKL 174
            EC+YVEPDE D+ KGVFWY NKL
Sbjct: 361  ECVYVEPDEGDRSKGVFWYKNKL 383


>gb|AFK31315.1| fatty acid desaturase [Camellia oleifera]
          Length = 383

 Score =  686 bits (1769), Expect = 0.0
 Identities = 312/383 (81%), Positives = 343/383 (89%), Gaps = 1/383 (0%)
 Frame = -2

Query: 1319 MGAGGRMSVPSKGKKSKSDVFQRVPCEKPPFTVGDIKKAIPPHCFQRSVLHSFSYVVYDL 1140
            MGAGGRMSVP +GKKS  DV +RVP  KPPFTVG+IKKAIPPHCF+RSVLHSFSYVVYDL
Sbjct: 1    MGAGGRMSVPPEGKKSDRDVIRRVPYSKPPFTVGEIKKAIPPHCFRRSVLHSFSYVVYDL 60

Query: 1139 TIAFLFYYAASNYIHLLPCPLSYLAWPIYWFCQGCVLTGVWVIAHECGHHAFSDYQWLDD 960
             IAFLFYY A+NYIHLLP PLSYLAW +YW CQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61   IIAFLFYYLATNYIHLLPQPLSYLAWLVYWICQGCVLTGVWVVAHECGHHAFSDYQWLDD 120

Query: 959  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKRKSGISWFLKYLNNPPGRIL 780
            TVGL+LHS+LLVPYFSWKYSHRRHHSNT SLERDEVFVPK KS + W+ KYLNNPPGRIL
Sbjct: 121  TVGLVLHSALLVPYFSWKYSHRRHHSNTASLERDEVFVPKLKSSVGWYSKYLNNPPGRIL 180

Query: 779  ILLVQLILGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIFISDAGITAMTYLLY 600
             +++ L LGWPLYLMFNVSGR YDRFACH+DP  PIY+DRERLQI++SDAG+  ++Y+LY
Sbjct: 181  TVVITLTLGWPLYLMFNVSGRHYDRFACHYDPYGPIYSDRERLQIYLSDAGVLGVSYVLY 240

Query: 599  RLVAAKGLSWVFCYYGAPLLIVNGYLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 420
            R    KGL+WV C YG PLLIVNG+LVLIT+LQHTHPALPHYDSSEWDWLRGALAT DRD
Sbjct: 241  RFALVKGLAWVLCLYGGPLLIVNGFLVLITWLQHTHPALPHYDSSEWDWLRGALATCDRD 300

Query: 419  YGFLNKVFHNITNTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVFKAMYREVK 240
            YG LNKVFHNIT+THVAHHLFSTMPHYHAMEATKAIKPILG+YYQ D TPV +A++RE K
Sbjct: 301  YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQCDATPVLRAIWREAK 360

Query: 239  ECIYVEPDETDQKKGVFWY-NKL 174
            ECIYVE DE+DQ KGVFWY NKL
Sbjct: 361  ECIYVENDESDQTKGVFWYKNKL 383


>gb|AAW63041.1| microsomal delta-12 oleate desaturase [Olea europaea]
          Length = 383

 Score =  682 bits (1761), Expect = 0.0
 Identities = 314/380 (82%), Positives = 339/380 (89%)
 Frame = -2

Query: 1319 MGAGGRMSVPSKGKKSKSDVFQRVPCEKPPFTVGDIKKAIPPHCFQRSVLHSFSYVVYDL 1140
            MGAGGRMS+P +GKKSKSDV QRVP  KP F++GDIKKAIPPHCFQRSVL SFSYVVYDL
Sbjct: 1    MGAGGRMSIPPEGKKSKSDVIQRVPQSKPQFSLGDIKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 1139 TIAFLFYYAASNYIHLLPCPLSYLAWPIYWFCQGCVLTGVWVIAHECGHHAFSDYQWLDD 960
            TIA L  Y AS YI  LP PLSY  W +YW CQGCVLTGVWV+AHECGHHAFSDYQWLDD
Sbjct: 61   TIASLLCYVASTYIQFLPNPLSYFGWLLYWICQGCVLTGVWVVAHECGHHAFSDYQWLDD 120

Query: 959  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKRKSGISWFLKYLNNPPGRIL 780
            TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVP+ KSGI W  K LNNPPGR+L
Sbjct: 121  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPRVKSGIRWISKNLNNPPGRVL 180

Query: 779  ILLVQLILGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIFISDAGITAMTYLLY 600
            +L+ QL LGWPLYLMFNVSGR YDRFACH++P SP+Y+DRER QI ISDAG+ A+T  LY
Sbjct: 181  LLVFQLTLGWPLYLMFNVSGRHYDRFACHYNPHSPMYSDRERAQILISDAGVLAVTCGLY 240

Query: 599  RLVAAKGLSWVFCYYGAPLLIVNGYLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 420
            RL AAKGL+WVFC YG PLL+VNG+LVLITYLQHTHP+LPHYDSSEWDWLRGALATVDRD
Sbjct: 241  RLTAAKGLAWVFCVYGGPLLVVNGFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300

Query: 419  YGFLNKVFHNITNTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVFKAMYREVK 240
            +G LNKVFHNIT+THV+HHLFSTMPHYHAMEATKAIKPILG YYQFDGTP+FKAM+RE K
Sbjct: 301  WGILNKVFHNITDTHVSHHLFSTMPHYHAMEATKAIKPILGNYYQFDGTPIFKAMWREAK 360

Query: 239  ECIYVEPDETDQKKGVFWYN 180
            ECIYVE DE DQ KGVFWYN
Sbjct: 361  ECIYVERDEGDQNKGVFWYN 380


>gb|AAN87573.1| delta 12 oleic acid desaturase FAD2 [Vernicia fordii]
          Length = 383

 Score =  675 bits (1741), Expect = 0.0
 Identities = 306/381 (80%), Positives = 337/381 (88%)
 Frame = -2

Query: 1319 MGAGGRMSVPSKGKKSKSDVFQRVPCEKPPFTVGDIKKAIPPHCFQRSVLHSFSYVVYDL 1140
            MGAGGRMSVP   KK +S+V +RVP  KPPFT+G +KKAIPPHCFQRSVL SFSYVVYDL
Sbjct: 1    MGAGGRMSVPPPPKKLESEVLKRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDL 60

Query: 1139 TIAFLFYYAASNYIHLLPCPLSYLAWPIYWFCQGCVLTGVWVIAHECGHHAFSDYQWLDD 960
            T+AF+FYY A+NY HLLP PLSY+AWPIYW  QGCVLTGVWVIAHECGHHAFSDYQ LDD
Sbjct: 61   TVAFIFYYIATNYFHLLPQPLSYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDD 120

Query: 959  TVGLILHSSLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKRKSGISWFLKYLNNPPGRIL 780
             VGL+LHS LLVPYFSWK+SHRRHHSNT SLERDEVFVPK+KS I WF KYLNNPPGR+ 
Sbjct: 121  IVGLVLHSCLLVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLF 180

Query: 779  ILLVQLILGWPLYLMFNVSGRPYDRFACHFDPKSPIYNDRERLQIFISDAGITAMTYLLY 600
             L + L LGWPLYL FNVSGRPYDRFACH+DP  PIY DRER +I+ISDAG+ A+T+ LY
Sbjct: 181  TLTITLTLGWPLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLY 240

Query: 599  RLVAAKGLSWVFCYYGAPLLIVNGYLVLITYLQHTHPALPHYDSSEWDWLRGALATVDRD 420
            RL AAKGL+WV C YG PLLIVN +LV+ITYLQHTHP++PHYDSSEWDWLRGALATVDRD
Sbjct: 241  RLAAAKGLAWVICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRD 300

Query: 419  YGFLNKVFHNITNTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVFKAMYREVK 240
            YG LNKVFHNIT+THVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTP +KAM+RE K
Sbjct: 301  YGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAK 360

Query: 239  ECIYVEPDETDQKKGVFWYNK 177
            ECIYVE D+ D+ KGV+WYNK
Sbjct: 361  ECIYVEADDGDESKGVYWYNK 381