BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00022642
         (1523 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desatur...   638   e-180
ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|2...   551   e-176
gb|ADC80921.1| delta-8 sphingolipid desaturase [Vernicia fordii]      550   e-176
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   548   e-175
ref|NP_191717.1| Fatty acid/sphingolipid desaturase [Arabidopsis...   547   e-175

>sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
            Full=Delta(8)-sphingolipid desaturase
            gi|12002282|gb|AAG43277.1|AF133728_1 delta 8-sphingolipid
            desaturase [Borago officinalis]
          Length = 446

 Score =  638 bits (1646), Expect = e-180
 Identities = 302/354 (85%), Positives = 315/354 (88%)
 Frame = -3

Query: 1371 RKLVSEFSKAGLFDKKGHLVIYCISFXXXXXXXXXXXXXCSESFWVHMLSAAMLGMCWIQ 1192
            RKL SEFSKAGLF+KKGH VIYC+SF             CS S WVHMLS AMLGMC+IQ
Sbjct: 93   RKLASEFSKAGLFEKKGHTVIYCLSFIALLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQ 152

Query: 1191 AAYLGHDSGHYNIMTTKGWNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 1012
            AAYLGHDSGHY +M++KG+NKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ
Sbjct: 153  AAYLGHDSGHYTMMSSKGYNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 212

Query: 1011 HLPFFAVSSRFFKSLTSCFYGRELTFDGASRFLVSYQHFTYYPVMIFARINLYVQTFLLL 832
            HLP FAV S FFKSLTS FYGRELTFDG SRFLVSYQHFT Y VMIF RINLYVQTFLLL
Sbjct: 213  HLPVFAVPSSFFKSLTSRFYGRELTFDGLSRFLVSYQHFTIYLVMIFGRINLYVQTFLLL 272

Query: 831  LSTRKVPDRALNIIGILVFWTWFPYLVSCLPNWNERVLFVVTSFSVTALQHVQFTLNHFA 652
             STRKVPDRALNIIGILV+WTWFPYLVSCLPNWNERVLFV+T FSVTALQH+QFTLNHFA
Sbjct: 273  FSTRKVPDRALNIIGILVYWTWFPYLVSCLPNWNERVLFVLTCFSVTALQHIQFTLNHFA 332

Query: 651  GSVYVGPPKGNDWFEKQTAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPI 472
              VYVGPP G +WFEKQ AGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLR ISPI
Sbjct: 333  ADVYVGPPTGTNWFEKQAAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRNISPI 392

Query: 471  IQDYCKKHELPYRSLSFFDANVETLKTLRTAALQARDLTEVPQNLLWEAVNTHG 310
            +QDYCKKH LPYRSLSFFDANV T+KTLRTAALQARDLT VPQNLLWEA NTHG
Sbjct: 393  VQDYCKKHNLPYRSLSFFDANVATIKTLRTAALQARDLTVVPQNLLWEAFNTHG 446



 Score =  105 bits (263), Expect = 2e-20
 Identities = 48/61 (78%), Positives = 53/61 (86%)
 Frame = -1

Query: 1523 QKHNKPGDVWISIQGKVYNVTDWIKEHPGGYVVIMNLAGQDATDAFIAYHPGSLFLNFRR 1344
            +KHN+ GDVWISIQGKVYNVTDWIK+HPGG V IMNLAGQDATDAFIAYHPG+ + N   
Sbjct: 14   EKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFIAYHPGTAWKNLEN 73

Query: 1343 L 1341
            L
Sbjct: 74   L 74


>ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|222844339|gb|EEE81886.1|
            predicted protein [Populus trichocarpa]
          Length = 447

 Score =  551 bits (1420), Expect(2) = e-176
 Identities = 249/355 (70%), Positives = 293/355 (82%), Gaps = 1/355 (0%)
 Frame = -3

Query: 1371 RKLVSEFSKAGLFDKKGHLVIYCISFXXXXXXXXXXXXXCSESFWVHMLSAAMLGMCWIQ 1192
            R+L SEF+K GLF+KKGH+ +Y ++              C +S W H+ SA +LG  WIQ
Sbjct: 93   RRLASEFAKLGLFEKKGHITMYALASIALMFCVVLYGVLCCQSVWAHLGSALVLGFLWIQ 152

Query: 1191 AAYLGHDSGHYNIMTTKGWNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 1012
            +AY+GHDSGHY +M ++G+NKFAQ + GN LTGISIAWWKWTHNAHH+ACNSLDYDPDLQ
Sbjct: 153  SAYIGHDSGHYQVMKSRGYNKFAQFVAGNSLTGISIAWWKWTHNAHHLACNSLDYDPDLQ 212

Query: 1011 HLPFFAVSSRFFKSLTSCFYGRELTFDGASRFLVSYQHFTYYPVMIFARINLYVQTFLLL 832
            H+P FAV+S FF S+ SCFYGR L FD  +RF VSYQH+T+YPVM  AR+NLY+QTFLLL
Sbjct: 213  HIPVFAVNSTFFNSIKSCFYGRYLDFDPVARFFVSYQHWTFYPVMCVARVNLYLQTFLLL 272

Query: 831  LSTRKVPDRALNIIGILVFWTWFPYLVSCLPNWNERVLFVVTSFSVTALQHVQFTLNHFA 652
             S RK PDRALNI+GIL+FWTWFP LVSCLPNW ERV+FV+ SF+VTA+QHVQF LNHFA
Sbjct: 273  FSKRKFPDRALNILGILIFWTWFPLLVSCLPNWPERVMFVLASFAVTAIQHVQFCLNHFA 332

Query: 651  GSVYVGPPKGNDWFEKQTAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPI 472
              VY GPPKGNDWFEKQT GT+DISCSSWMDWF+GGLQFQLEHHLFPRMPRCQLR++SP+
Sbjct: 333  ADVYTGPPKGNDWFEKQTGGTLDISCSSWMDWFYGGLQFQLEHHLFPRMPRCQLRRVSPL 392

Query: 471  IQDYCKKHELPYRSLSFFDANVETLKTLRTAALQARDLTE-VPQNLLWEAVNTHG 310
            +QD CKKH L YRSLSF++ANV T++ LR  A QARDL+  VP+NLLWEAVNTHG
Sbjct: 393  VQDLCKKHNLSYRSLSFWEANVWTIRRLRNVAQQARDLSNPVPKNLLWEAVNTHG 447



 Score = 94.7 bits (234), Expect(2) = e-176
 Identities = 40/53 (75%), Positives = 47/53 (88%)
 Frame = -1

Query: 1523 QKHNKPGDVWISIQGKVYNVTDWIKEHPGGYVVIMNLAGQDATDAFIAYHPGS 1365
            ++HNK GD+WISIQGKVYNV+DW  EHPGG V ++NLAGQD TDAFIAYHPG+
Sbjct: 14   KQHNKEGDLWISIQGKVYNVSDWAYEHPGGDVALLNLAGQDVTDAFIAYHPGT 66


>gb|ADC80921.1| delta-8 sphingolipid desaturase [Vernicia fordii]
          Length = 447

 Score =  550 bits (1417), Expect(2) = e-176
 Identities = 247/355 (69%), Positives = 296/355 (83%), Gaps = 1/355 (0%)
 Frame = -3

Query: 1371 RKLVSEFSKAGLFDKKGHLVIYCISFXXXXXXXXXXXXXCSESFWVHMLSAAMLGMCWIQ 1192
            RKL  EF+K GLFD KGH+ +Y ++                +SFW HM SA  LG+ WI 
Sbjct: 93   RKLNLEFAKLGLFDMKGHVALYSLASVVFLFYIVVYGVLYCQSFWAHMGSAVSLGILWII 152

Query: 1191 AAYLGHDSGHYNIMTTKGWNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 1012
            +AY+GHDSGHY +M +  +NK AQ+L+GNCLTGISIAWWKWTHNAHH+ACNSLDYDPDLQ
Sbjct: 153  SAYVGHDSGHYQVMLSPAFNKLAQLLSGNCLTGISIAWWKWTHNAHHLACNSLDYDPDLQ 212

Query: 1011 HLPFFAVSSRFFKSLTSCFYGRELTFDGASRFLVSYQHFTYYPVMIFARINLYVQTFLLL 832
            H+PFFAVSS  F S+ SCFYGR + FD  +RF VSYQH+T+YPVM  AR+NLY+QT LLL
Sbjct: 213  HIPFFAVSSNLFNSIRSCFYGRNMNFDPLARFFVSYQHWTFYPVMCVARVNLYLQTLLLL 272

Query: 831  LSTRKVPDRALNIIGILVFWTWFPYLVSCLPNWNERVLFVVTSFSVTALQHVQFTLNHFA 652
             S RKVPDRALNI+GILVFWTWFP LVSCLP+W ERV+FV+TSF+VT+LQH+QF LNHF+
Sbjct: 273  FSNRKVPDRALNIMGILVFWTWFPLLVSCLPDWPERVIFVLTSFAVTSLQHIQFCLNHFS 332

Query: 651  GSVYVGPPKGNDWFEKQTAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPI 472
             +VY+GPP GNDWFEKQT+GT+DISCSSWMDW +GGLQFQLEHHLFPR+PRCQLRK+SP+
Sbjct: 333  ANVYLGPPNGNDWFEKQTSGTLDISCSSWMDWAYGGLQFQLEHHLFPRLPRCQLRKVSPL 392

Query: 471  IQDYCKKHELPYRSLSFFDANVETLKTLRTAALQARDLTE-VPQNLLWEAVNTHG 310
            ++D CKKH LPYRSLSF++ANV T++TLR AALQARD+T  VP+NL+WEAV+THG
Sbjct: 393  VRDLCKKHNLPYRSLSFWEANVWTIRTLRIAALQARDMTNPVPKNLVWEAVHTHG 447



 Score = 95.1 bits (235), Expect(2) = e-176
 Identities = 38/53 (71%), Positives = 49/53 (92%)
 Frame = -1

Query: 1523 QKHNKPGDVWISIQGKVYNVTDWIKEHPGGYVVIMNLAGQDATDAFIAYHPGS 1365
            ++HN+ GD+WISIQGKVYNV+DW+KEHPGG + ++NLAGQD TDAF+AYHPG+
Sbjct: 14   KQHNRHGDLWISIQGKVYNVSDWVKEHPGGDIPLLNLAGQDVTDAFMAYHPGT 66


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  548 bits (1413), Expect(2) = e-175
 Identities = 250/355 (70%), Positives = 293/355 (82%), Gaps = 1/355 (0%)
 Frame = -3

Query: 1371 RKLVSEFSKAGLFDKKGHLVIYCISFXXXXXXXXXXXXXCSESFWVHMLSAAMLGMCWIQ 1192
            RKL +EFSK GLF+KKGH ++Y  S              C +SFWVH+  AA+LG  WIQ
Sbjct: 93   RKLANEFSKLGLFEKKGHGILYTCSIIGFLFMVCIYGVLCVDSFWVHLGCAALLGFLWIQ 152

Query: 1191 AAYLGHDSGHYNIMTTKGWNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 1012
            ++YLGHDSGHY IM++ G+NK AQ+L GNC+TGISIAWWKWTHNAHHIACNSLDYDPDLQ
Sbjct: 153  SSYLGHDSGHYQIMSSPGFNKLAQMLIGNCITGISIAWWKWTHNAHHIACNSLDYDPDLQ 212

Query: 1011 HLPFFAVSSRFFKSLTSCFYGRELTFDGASRFLVSYQHFTYYPVMIFARINLYVQTFLLL 832
            H+P FAVSS  F S+ S FYGR L FD  +RF VSYQH+TYYPVM  ARINL++QT LLL
Sbjct: 213  HIPVFAVSSSLFDSINSVFYGRILKFDSVARFFVSYQHWTYYPVMCVARINLFLQTLLLL 272

Query: 831  LSTRKVPDRALNIIGILVFWTWFPYLVSCLPNWNERVLFVVTSFSVTALQHVQFTLNHFA 652
             S R +PDRALNI+GI VFWTW+P LVSCLPNW ERV+FV+ S +VT++QH+QFTLNHF+
Sbjct: 273  FSGRNIPDRALNIMGICVFWTWYPLLVSCLPNWGERVMFVIVSMAVTSIQHIQFTLNHFS 332

Query: 651  GSVYVGPPKGNDWFEKQTAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPI 472
             +VYVGPP GNDWFEKQT+GTIDISCS+WMDWF GGLQFQLEHHLFPR+PRC LR ISP+
Sbjct: 333  ANVYVGPPCGNDWFEKQTSGTIDISCSAWMDWFHGGLQFQLEHHLFPRLPRCHLRSISPV 392

Query: 471  IQDYCKKHELPYRSLSFFDANVETLKTLRTAALQARDLTE-VPQNLLWEAVNTHG 310
            ++D CKKH LPYRSLSF++ANV T++TLRTAALQARDLT  VP+NL+WEA N HG
Sbjct: 393  VKDLCKKHNLPYRSLSFWEANVSTIRTLRTAALQARDLTNPVPKNLVWEAANIHG 447



 Score = 96.3 bits (238), Expect(2) = e-175
 Identities = 40/53 (75%), Positives = 48/53 (90%)
 Frame = -1

Query: 1523 QKHNKPGDVWISIQGKVYNVTDWIKEHPGGYVVIMNLAGQDATDAFIAYHPGS 1365
            ++H+KPGD+WISIQGKVYNVTDW K HPGG + ++NLAGQD TDAFIAYHPG+
Sbjct: 14   KQHDKPGDLWISIQGKVYNVTDWAKVHPGGDIPLLNLAGQDVTDAFIAYHPGT 66


>ref|NP_191717.1| Fatty acid/sphingolipid desaturase [Arabidopsis thaliana]
            gi|16226517|gb|AAL16189.1|AF428420_1 AT3g61580/F2A19_180
            [Arabidopsis thaliana] gi|3819710|emb|CAA11858.1| delta-8
            sphingolipid desaturase [Arabidopsis thaliana]
            gi|6850849|emb|CAB71088.1| delta-8 sphingolipid
            desaturase [Arabidopsis thaliana]
            gi|23306384|gb|AAN17419.1| delta-8 sphingolipid
            desaturase [Arabidopsis thaliana]
            gi|28059272|gb|AAO30042.1| delta-8 sphingolipid
            desaturase [Arabidopsis thaliana]
            gi|332646705|gb|AEE80226.1| Fatty acid/sphingolipid
            desaturase [Arabidopsis thaliana]
          Length = 449

 Score =  547 bits (1409), Expect(2) = e-175
 Identities = 247/355 (69%), Positives = 294/355 (82%), Gaps = 1/355 (0%)
 Frame = -3

Query: 1371 RKLVSEFSKAGLFDKKGHLVIYCISFXXXXXXXXXXXXXCSESFWVHMLSAAMLGMCWIQ 1192
            R++ +EF K GLF+ KGH+ +Y ++F                S + H ++AA+LG+ WIQ
Sbjct: 95   RRMAAEFRKLGLFENKGHVTLYTLAFVAAMFLGVLYGVLACTSVFAHQIAAALLGLLWIQ 154

Query: 1191 AAYLGHDSGHYNIMTTKGWNKFAQVLNGNCLTGISIAWWKWTHNAHHIACNSLDYDPDLQ 1012
            +AY+GHDSGHY IM+ K +N+FAQ+L+GNCLTGISIAWWKWTHNAHH+ACNSLDYDPDLQ
Sbjct: 155  SAYIGHDSGHYVIMSNKSYNRFAQLLSGNCLTGISIAWWKWTHNAHHLACNSLDYDPDLQ 214

Query: 1011 HLPFFAVSSRFFKSLTSCFYGRELTFDGASRFLVSYQHFTYYPVMIFARINLYVQTFLLL 832
            H+P FAVS++FF SLTS FY R+LTFD  +RFLVSYQHFTYYPVM F RINL++QTFLLL
Sbjct: 215  HIPVFAVSTKFFSSLTSRFYDRKLTFDPVARFLVSYQHFTYYPVMCFGRINLFIQTFLLL 274

Query: 831  LSTRKVPDRALNIIGILVFWTWFPYLVSCLPNWNERVLFVVTSFSVTALQHVQFTLNHFA 652
             S R+VPDRALN  GILVFWTWFP LVSCLPNW ER  FV TSF+VTALQH+QFTLNHFA
Sbjct: 275  FSKREVPDRALNFAGILVFWTWFPLLVSCLPNWPERFFFVFTSFTVTALQHIQFTLNHFA 334

Query: 651  GSVYVGPPKGNDWFEKQTAGTIDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPI 472
              VYVGPP G+DWFEKQ AGTIDISC S+MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+
Sbjct: 335  ADVYVGPPTGSDWFEKQAAGTIDISCRSYMDWFFGGLQFQLEHHLFPRLPRCHLRKVSPV 394

Query: 471  IQDYCKKHELPYRSLSFFDANVETLKTLRTAALQARDLTE-VPQNLLWEAVNTHG 310
            +Q+ CKKH LPYRS+S+F+ANV T+ TL+TAA QARD+   V +NL+WEA+NTHG
Sbjct: 395  VQELCKKHNLPYRSMSWFEANVLTINTLKTAAYQARDVANPVVKNLVWEALNTHG 449



 Score = 95.1 bits (235), Expect(2) = e-175
 Identities = 40/53 (75%), Positives = 47/53 (88%)
 Frame = -1

Query: 1523 QKHNKPGDVWISIQGKVYNVTDWIKEHPGGYVVIMNLAGQDATDAFIAYHPGS 1365
            +KHNK GD+WI+IQGKVYNV+DWIK HPGG  VI+NL GQD TDAFIA+HPG+
Sbjct: 16   KKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIAFHPGT 68