BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00020847
         (1067 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8L6J1.2|RPO3B_TOBAC RecName: Full=DNA-directed RNA polymeras...   201   3e-49
sp|P69242.1|RPOT3_NICSY RecName: Full=DNA-directed RNA polymeras...   198   2e-48
ref|XP_004162698.1| PREDICTED: DNA-directed RNA polymerase 3, ch...   173   6e-41
ref|XP_004144791.1| PREDICTED: DNA-directed RNA polymerase 3, ch...   171   4e-40
emb|CAC80139.1| DNA-dependent RNA polymerase [Spinacia oleracea]      167   3e-39

>sp|Q8L6J1.2|RPO3B_TOBAC RecName: Full=DNA-directed RNA polymerase 3B, chloroplastic; AltName:
            Full=NictaRpoT3-tom; AltName: Full=T7 bacteriophage-type
            single subunit RNA polymerase 3B; Flags: Precursor
            gi|21425667|emb|CAC95021.2| chloroplast RNA polymerase
            [Nicotiana tabacum]
          Length = 977

 Score =  201 bits (510), Expect = 3e-49
 Identities = 109/204 (53%), Positives = 136/204 (66%), Gaps = 7/204 (3%)
 Frame = +2

Query: 476  LENSSKRLFVQESSEKYTQESCNLQKIPNGVAQKVIENSSKRVFIQDPPWISSLFMKNLY 655
            + N   +  V  S+++  +E  NLQKIPNG   K    S+KRVFIQDPPW+SSLFM +L+
Sbjct: 61   INNIQSQTTVCVSTDENLEELVNLQKIPNGFLNK---ESNKRVFIQDPPWVSSLFMNSLF 117

Query: 656  ------NGVKLEFREMERRK-YNFLRKRQVKAETEAWXXXXXXXXXXXXXXXXKKLAPNL 814
                   GV+ EFRE+ERR+ Y  LR+RQ+KAETEAW                KKLAPNL
Sbjct: 118  VRAKQVQGVRREFREIERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREMCEKKLAPNL 177

Query: 815  PCIKKMFLNWFEPLRVAIEREXXXXXXXXXXXXXXPNIDCLPADKMAVIVMHKLMALLMM 994
            P +KK+ L WFEPLR AIE+E              P+ID LPADKMAVIVMHKLM LLMM
Sbjct: 178  PYVKKLLLGWFEPLRQAIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVMHKLMGLLMM 237

Query: 995  GGKDERSVRVVEAAIQIGVSIEHE 1066
            GGK+ER V+VV+AA+QIG+++E+E
Sbjct: 238  GGKEERCVQVVQAAVQIGMAVENE 261



 Score = 57.0 bits (136), Expect = 8e-06
 Identities = 44/105 (41%), Positives = 63/105 (60%), Gaps = 5/105 (4%)
 Frame = +2

Query: 119 APFTPSPRAQW-SKSNPKKLQ--IKHSNFIFASFSEQIIFSKTSSFSFPHTLKNLPNLHS 289
           A ++PSP +QW ++  PK+    + H N  F   S+    S  S+ SFP TLK LP +H 
Sbjct: 5   ASYSPSPTSQWRTQKLPKRFNFYVIH-NQEFGKLSQS---SSLSTSSFPKTLK-LPVIHM 59

Query: 290 HIHHIKIQP-LHDSTENYTQELCNLQKIPQKML-ENSSKRVFLQD 418
            I++I+ Q  +  ST+   +EL NLQKIP   L + S+KRVF+QD
Sbjct: 60  PINNIQSQTTVCVSTDENLEELVNLQKIPNGFLNKESNKRVFIQD 104


>sp|P69242.1|RPOT3_NICSY RecName: Full=DNA-directed RNA polymerase 3, chloroplastic; AltName:
            Full=NsRpoT-C; AltName: Full=T7 bacteriophage-type single
            subunit RNA polymerase 3; Flags: Precursor
            gi|59800203|sp|P69243.1|RPO3A_TOBAC RecName:
            Full=DNA-directed RNA polymerase 3A, chloroplastic;
            AltName: Full=NictaRpoT3-syl; AltName: Full=T7
            bacteriophage-type single subunit RNA polymerase 3A;
            Flags: Precursor gi|21425641|emb|CAC82576.3| phage-type
            RNA polymerase [Nicotiana sylvestris]
            gi|21425679|emb|CAC95027.2| plastid RNA polymerase
            [Nicotiana tabacum] gi|24475541|dbj|BAC22693.1| T7
            bacteriophage-type single subunit RNA polymerase
            [Nicotiana sylvestris] gi|24475543|dbj|BAC22694.1| T7
            bacteriophage-type single subunit RNA polymerase
            [Nicotiana sylvestris]
          Length = 977

 Score =  198 bits (504), Expect = 2e-48
 Identities = 109/202 (53%), Positives = 135/202 (66%), Gaps = 7/202 (3%)
 Frame = +2

Query: 482  NSSKRLFVQESSEKYTQESCNLQKIPNGVAQKVIENSSKRVFIQDPPWISSLFMKNLY-- 655
            N   +  V  S+++  +E  NLQKI NGV  K    S+KRVFIQDPPW+SSLFM +L+  
Sbjct: 64   NIQSQTTVCVSTDENLEELVNLQKIANGVLTK---ESNKRVFIQDPPWVSSLFMNSLFVR 120

Query: 656  ----NGVKLEFREMERRK-YNFLRKRQVKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPC 820
                 GV+ EFRE+ERR+ Y  LR+RQ+KAETEAW                KKLAPNLP 
Sbjct: 121  AKQVQGVRREFREIERRRRYAMLRRRQIKAETEAWEQMVEEYRELEREMCEKKLAPNLPY 180

Query: 821  IKKMFLNWFEPLRVAIEREXXXXXXXXXXXXXXPNIDCLPADKMAVIVMHKLMALLMMGG 1000
            +KK+ L WFEPLR AIE+E              P+ID LPADKMAVIVMHKLM LLMMGG
Sbjct: 181  VKKLLLGWFEPLRQAIEKEQNAETTVKHRAAFAPHIDSLPADKMAVIVMHKLMGLLMMGG 240

Query: 1001 KDERSVRVVEAAIQIGVSIEHE 1066
            K+ER V+VV+AA+QIG+++E+E
Sbjct: 241  KEERCVQVVQAAVQIGMAVENE 262


>ref|XP_004162698.1| PREDICTED: DNA-directed RNA polymerase 3, chloroplastic-like [Cucumis
            sativus]
          Length = 975

 Score =  173 bits (439), Expect = 6e-41
 Identities = 98/192 (51%), Positives = 121/192 (63%), Gaps = 4/192 (2%)
 Frame = +2

Query: 503  VQESSEKYTQESCNLQKIPNGVAQKVIENSSKRVFIQDPPWISSLFMKNLYN----GVKL 670
            V E+  +    S NLQ   + + Q     SSKR+FIQDPPWI+SLF+K LY      VKL
Sbjct: 71   VHENFTETLDNSMNLQIPISEIHQITRGESSKRIFIQDPPWIASLFLKGLYKCVQKEVKL 130

Query: 671  EFREMERRKYNFLRKRQVKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPCIKKMFLNWFE 850
            EF E+E+RKYN LR+RQ+KAETEAW                KKLAPNLP +KK+FL WFE
Sbjct: 131  EFMEIEKRKYNLLRRRQIKAETEAWEKMVEEYKELEREMCEKKLAPNLPYVKKLFLGWFE 190

Query: 851  PLRVAIEREXXXXXXXXXXXXXXPNIDCLPADKMAVIVMHKLMALLMMGGKDERSVRVVE 1030
            PL+ AIE E              P+I+ L ADKMAVIVM+K+M LLMM  +D   V+VV+
Sbjct: 191  PLKNAIEEEQKTQRSKKQKAAFAPHIELLSADKMAVIVMYKMMGLLMMAAQD-GCVQVVQ 249

Query: 1031 AAIQIGVSIEHE 1066
            AA+QIG +IE E
Sbjct: 250  AAVQIGGAIEQE 261


>ref|XP_004144791.1| PREDICTED: DNA-directed RNA polymerase 3, chloroplastic-like [Cucumis
            sativus]
          Length = 975

 Score =  171 bits (432), Expect = 4e-40
 Identities = 97/192 (50%), Positives = 120/192 (62%), Gaps = 4/192 (2%)
 Frame = +2

Query: 503  VQESSEKYTQESCNLQKIPNGVAQKVIENSSKRVFIQDPPWISSLFMKNLYN----GVKL 670
            V E+  +    S NLQ   + + Q     SSKR+FIQDPPWI+SLF+K LY      VKL
Sbjct: 71   VHENFTETLDNSMNLQIPISEIHQITRGESSKRIFIQDPPWIASLFLKGLYKCVQKEVKL 130

Query: 671  EFREMERRKYNFLRKRQVKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPCIKKMFLNWFE 850
            EF E+E+RKYN LR+RQ+KAETEAW                KKLAPNLP +KK+FL WFE
Sbjct: 131  EFMEIEKRKYNLLRRRQIKAETEAWEKMVEEYKELEREMCEKKLAPNLPYVKKLFLGWFE 190

Query: 851  PLRVAIEREXXXXXXXXXXXXXXPNIDCLPADKMAVIVMHKLMALLMMGGKDERSVRVVE 1030
            PL+ AIE E              P+I+ L ADKMA IVM+K+M LLMM  +D   V+VV+
Sbjct: 191  PLKNAIEEEQKTQRSKKQKAAFAPHIELLSADKMAGIVMYKMMGLLMMAAQD-GCVQVVQ 249

Query: 1031 AAIQIGVSIEHE 1066
            AA+QIG +IE E
Sbjct: 250  AAVQIGGAIEQE 261


>emb|CAC80139.1| DNA-dependent RNA polymerase [Spinacia oleracea]
          Length = 989

 Score =  167 bits (424), Expect = 3e-39
 Identities = 123/321 (38%), Positives = 163/321 (50%), Gaps = 5/321 (1%)
 Frame = +2

Query: 119  APFTPSPRAQWSKSNPKKLQIKHSNFIFASFSEQIIFSKTSSFSF--PHTLKNLPNLHSH 292
            A F+P P+ Q  K   K L+  H              SKT+ FS   P + K L      
Sbjct: 5    ASFSPIPQVQIQKQWRKPLK-SHKKA-----------SKTTHFSLFIPLSSKQL-----R 47

Query: 293  IHHIKIQPLHDSTENYTQELCNLQKIPQKMLENSSKRVFLQDSSENYTQELCNLQKNSQK 472
            I H  + P   S+ N      +L  +  K+     K  F Q S +            SQ 
Sbjct: 48   IEH-PLFPSSSSSSNSFPSTLSLPSLTPKL----GKTSFFQPSPQ------------SQI 90

Query: 473  MLENSSKRLFVQESSEKYTQESCNLQKIPNGVAQKVIENSSKRVFIQDPPWISSLFMKNL 652
            +L++  + L     +EK      N  KI            S R+F+QDPPW+++LFMK L
Sbjct: 91   ILDSPEEEL-----AEKMDGHFRNSDKI----------KGSSRIFMQDPPWVNTLFMKGL 135

Query: 653  YN---GVKLEFREMERRKYNFLRKRQVKAETEAWXXXXXXXXXXXXXXXXKKLAPNLPCI 823
             +   G++LEF+E+ER KYN LR+RQ+KAETEAW                KKLAPNLP +
Sbjct: 136  VDSNTGLRLEFKEIEREKYNLLRRRQIKAETEAWESMVEEYREFVREMRDKKLAPNLPQV 195

Query: 824  KKMFLNWFEPLRVAIEREXXXXXXXXXXXXXXPNIDCLPADKMAVIVMHKLMALLMMGGK 1003
            K +FL WFEPL+ AIE+E               +ID LPADKMAVIVMHK+M+LLM+ G 
Sbjct: 196  KALFLGWFEPLKKAIEKEQKSQRSRKQKAIYGYHIDMLPADKMAVIVMHKMMSLLMV-GN 254

Query: 1004 DERSVRVVEAAIQIGVSIEHE 1066
            D   VR+V+AA+QIG +IE E
Sbjct: 255  DAGCVRLVQAALQIGTAIEQE 275