BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00020774
         (944 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       296   5e-78
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   294   2e-77
ref|XP_002267397.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   289   8e-76
gb|ADD84651.1| CYP81B36 [Scoparia dulcis]                             289   8e-76
ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   288   1e-75

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  296 bits (758), Expect = 5e-78
 Identities = 148/308 (48%), Positives = 213/308 (69%)
 Frame = -1

Query: 944  VKSTANIKEKASLLDFLPLMRFFGLKNVEPKLKDIQMKRDEFMQRLVEEHRRTMSNDGNE 765
            V+ T  I   +++ DFLP++ + G+K +E K+ +++ KRD+F+Q L+EEHR + S     
Sbjct: 206  VQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHRISRSGGMQT 265

Query: 764  SKKRKSVIEVLLDLHEKDPEYYNDELIRNLILVLLQAGSDTSAITLERVFHHLVNNLNIL 585
             +K  ++I+VLL L E +PEYY DE+I  +I VLL AG+DTSA+T+E     L+NN  ++
Sbjct: 266  KEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVI 325

Query: 584  KQAQSDIDNLISGQDYHLVNESEISKLPLIRNIINETLRLDPAAPLLVPHRSSKECNVGG 405
            K+AQ++IDN +  +   L+NES+++KLP + +II ETLR+ PA PLLVPH SS+EC VGG
Sbjct: 326  KKAQAEIDNNL--EQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESSEECIVGG 383

Query: 404  YQIPSNTILLVNAWGMHHDPTVWEDPEKFKPERFQDYYFDQLKGGNMEFDGSKFISFGSG 225
            Y++PS T+LLVN W +  DP +W +P KFKPER     FD  +G     DG K + FGSG
Sbjct: 384  YKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPER-----FDGFEGTR---DGFKLMPFGSG 435

Query: 224  RRACPGENVAYKVMGLALGSLIQCFDWEKVSEDKILEINKKGNVGAPKVQPLMARCYPRP 45
            RR CPGE +A +V+ LALG+LIQCFDWE+V E+ ++++++   +  PKV PL A+C PR 
Sbjct: 436  RRGCPGEGLAMRVVALALGALIQCFDWERVGEE-MVDMSEGPGLTLPKVHPLEAKCRPRS 494

Query: 44   NTEMLISQ 21
                 ISQ
Sbjct: 495  TALNFISQ 502


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  294 bits (752), Expect = 2e-77
 Identities = 149/309 (48%), Positives = 212/309 (68%)
 Frame = -1

Query: 944  VKSTANIKEKASLLDFLPLMRFFGLKNVEPKLKDIQMKRDEFMQRLVEEHRRTMSNDGNE 765
            V  T  +    +++DFLP++ + GLK  E +L  +Q +R+ F+Q L+EEHRR  SN    
Sbjct: 205  VSETFQLAGATNMVDFLPILGWLGLKGTERRLIKLQKRRESFIQNLIEEHRRKGSNCEG- 263

Query: 764  SKKRKSVIEVLLDLHEKDPEYYNDELIRNLILVLLQAGSDTSAITLERVFHHLVNNLNIL 585
              ++K++IEVLL L E +PEYY DE+IR L+L +L  G+DTSA T+E     L+NN  +L
Sbjct: 264  --RQKTMIEVLLSLQETEPEYYTDEIIRGLMLSMLTGGTDTSAGTMEWALSLLLNNPKVL 321

Query: 584  KQAQSDIDNLISGQDYHLVNESEISKLPLIRNIINETLRLDPAAPLLVPHRSSKECNVGG 405
            K+A  +ID+ + G D  L+ E ++++LP +R+II ETLR+ PA PLLVPH SSKEC+VGG
Sbjct: 322  KKAHQEIDDRL-GHD-RLIEELDLAQLPYLRSIIKETLRMYPAGPLLVPHESSKECSVGG 379

Query: 404  YQIPSNTILLVNAWGMHHDPTVWEDPEKFKPERFQDYYFDQLKGGNMEFDGSKFISFGSG 225
            ++IP  T+LLVN W +  D  +W DP +F+PERF+    D+        DG KF+ FGSG
Sbjct: 380  FRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPERFEGVEGDR--------DGFKFVPFGSG 431

Query: 224  RRACPGENVAYKVMGLALGSLIQCFDWEKVSEDKILEINKKGNVGAPKVQPLMARCYPRP 45
            RR CPGE +A +++GLALGSLIQCFDWE+V E +++++ + G +  PK QPL+A+C PRP
Sbjct: 432  RRGCPGEALAIRIVGLALGSLIQCFDWERVDE-QMVDMTEGGGLTLPKAQPLLAKCRPRP 490

Query: 44   NTEMLISQM 18
                L SQ+
Sbjct: 491  TMVNLFSQL 499


>ref|XP_002267397.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 560

 Score =  289 bits (739), Expect = 8e-76
 Identities = 146/319 (45%), Positives = 212/319 (66%), Gaps = 11/319 (3%)
 Frame = -1

Query: 944  VKSTANIKEKASLLDFLPLMRFFGLKNVEPKLKDIQMKRDEFMQRLVEEHRRTMSN---- 777
            +  T  + +  ++ D+LP++R+ G+K +E  L+++Q KRD FMQ L+EEHR  M+     
Sbjct: 253  IADTLRLGDTTNVGDYLPMLRWLGVKGMEKGLRELQRKRDRFMQSLIEEHRTRMAEEKES 312

Query: 776  -------DGNESKKRKSVIEVLLDLHEKDPEYYNDELIRNLILVLLQAGSDTSAITLERV 618
                   D  E KK+K++IEV+L L EK+P+YY D++IR L+LVLL AG+DT+A T+E  
Sbjct: 313  YSSCSNGDDGEKKKKKTMIEVMLSLQEKEPDYYTDQIIRGLMLVLLGAGTDTTATTIEWT 372

Query: 617  FHHLVNNLNILKQAQSDIDNLISGQDYHLVNESEISKLPLIRNIINETLRLDPAAPLLVP 438
               L+NN + LK+AQ +IDN +     HL+ ES++++LP +  II E+ R+ PA P ++P
Sbjct: 373  LSLLLNNPHALKKAQMEIDNHLGNN--HLIQESDLNQLPYLHCIIKESQRMYPAGP-IIP 429

Query: 437  HRSSKECNVGGYQIPSNTILLVNAWGMHHDPTVWEDPEKFKPERFQDYYFDQLKGGNMEF 258
            H SS EC VGGY+IP  T+LLVN W + +DP VWE+P KF PERF+        G  +E 
Sbjct: 430  HESSGECTVGGYRIPHGTMLLVNLWAIQNDPRVWEEPRKFMPERFE--------GIELEK 481

Query: 257  DGSKFISFGSGRRACPGENVAYKVMGLALGSLIQCFDWEKVSEDKILEINKKGNVGAPKV 78
             G + + FGSGRR CPGE +A +++GL LGSLIQCFDWE V E  ++++++   +  PK 
Sbjct: 482  HGFRLMPFGSGRRGCPGEGLALRMVGLVLGSLIQCFDWESVGEG-MVDMSEGTGLTLPKA 540

Query: 77   QPLMARCYPRPNTEMLISQ 21
            QPL+ RC PRP    L+S+
Sbjct: 541  QPLLVRCRPRPAFVDLLSK 559


>gb|ADD84651.1| CYP81B36 [Scoparia dulcis]
          Length = 502

 Score =  289 bits (739), Expect = 8e-76
 Identities = 149/298 (50%), Positives = 211/298 (70%)
 Frame = -1

Query: 911  SLLDFLPLMRFFGLKNVEPKLKDIQMKRDEFMQRLVEEHRRTMSNDGNESKKRKSVIEVL 732
            S+ DFLP++R+ G+   E  L ++Q KRD FMQ LVEE +R + N   +S    ++IE+L
Sbjct: 217  SMGDFLPIVRWLGIGGAEKGLAELQRKRDGFMQELVEECKRRIRNSSGDSGPM-TMIEML 275

Query: 731  LDLHEKDPEYYNDELIRNLILVLLQAGSDTSAITLERVFHHLVNNLNILKQAQSDIDNLI 552
            L L EK+PEYY D+LIR+L+L LL AG+DT+A T+E V   L+NN +++K+AQ +IDN I
Sbjct: 276  LPLQEKEPEYYTDKLIRSLMLTLLVAGTDTTAGTMEWVVSLLLNNPHVIKKAQVEIDNHI 335

Query: 551  SGQDYHLVNESEISKLPLIRNIINETLRLDPAAPLLVPHRSSKECNVGGYQIPSNTILLV 372
                 HL++ES+I+ LP +R I+NETLRL PA PLLVPH SS++C VGGY++P+ T+LLV
Sbjct: 336  GHG--HLIDESDITDLPYLRCIVNETLRLYPAGPLLVPHESSEQCIVGGYRVPAGTMLLV 393

Query: 371  NAWGMHHDPTVWEDPEKFKPERFQDYYFDQLKGGNMEFDGSKFISFGSGRRACPGENVAY 192
            N W +  DP  W++PEKFKPERF+      L+G     DG KF  FGSGRR CPGE +A 
Sbjct: 394  NLWAIQRDPKYWDEPEKFKPERFEG-----LEGNR---DGFKFSPFGSGRRGCPGEGLAV 445

Query: 191  KVMGLALGSLIQCFDWEKVSEDKILEINKKGNVGAPKVQPLMARCYPRPNTEMLISQM 18
            +++  ++GS+IQCFDWE+V ++ ++++++   +  PK QPLMA C  RP    L+SQ+
Sbjct: 446  RMLASSIGSIIQCFDWERVGKE-LVDMSEGVGLTLPKAQPLMAYCRARPLAAKLLSQI 502


>ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 507

 Score =  288 bits (738), Expect = 1e-75
 Identities = 148/314 (47%), Positives = 212/314 (67%), Gaps = 5/314 (1%)
 Frame = -1

Query: 944  VKSTANIKEKASLLDFLPLMRFFGLKNVEPKLKDIQMKRDEFMQRLVEEHRRTMSNDGNE 765
            ++ T  + +  ++ D+LP++R+ G+K  E  L+++Q KRD FMQ L+EEHR  M+ +   
Sbjct: 205  IEDTFRLGDTTNIGDYLPVLRWLGVKGKEKGLRELQRKRDRFMQGLIEEHRTRMAKESYS 264

Query: 764  SK-----KRKSVIEVLLDLHEKDPEYYNDELIRNLILVLLQAGSDTSAITLERVFHHLVN 600
            S      ++K++IEVLL L EK+ EYY DE+IR L+L LL AG+DT++ TLE     L+N
Sbjct: 265  SSSCRVGEKKTMIEVLLSLQEKEAEYYTDEIIRGLMLALLGAGTDTTSATLEWAMSLLLN 324

Query: 599  NLNILKQAQSDIDNLISGQDYHLVNESEISKLPLIRNIINETLRLDPAAPLLVPHRSSKE 420
            N  +LK+AQ ++DN +     HL+ ES++S+LP +  II ET R+ PA P+ VPH SSKE
Sbjct: 325  NPEVLKKAQMEMDNQLGPN--HLIEESDLSQLPYLHCIIRETQRMYPAGPI-VPHESSKE 381

Query: 419  CNVGGYQIPSNTILLVNAWGMHHDPTVWEDPEKFKPERFQDYYFDQLKGGNMEFDGSKFI 240
            C VGGY IP  T+LLVN WG+ +DP VW++P KF PERF+        G   E  G + +
Sbjct: 382  CMVGGYHIPRGTMLLVNIWGIQNDPKVWKEPRKFLPERFE-------VGLEGEGHGLRLM 434

Query: 239  SFGSGRRACPGENVAYKVMGLALGSLIQCFDWEKVSEDKILEINKKGNVGAPKVQPLMAR 60
             FGSGRR CPGE +A +++GL LGSLIQCFDWE+V E K+ ++++   +  PK QPL+A+
Sbjct: 435  PFGSGRRGCPGEGLAIRMVGLVLGSLIQCFDWERVGEGKV-DMSEGIGLTLPKAQPLLAK 493

Query: 59   CYPRPNTEMLISQM 18
            C PRP    ++SQ+
Sbjct: 494  CRPRPALINVLSQI 507