BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00019869
         (1533 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricin...   525   e-172
emb|CBI31610.3| unnamed protein product [Vitis vinifera]              520   e-171
ref|XP_003544172.1| PREDICTED: folylpolyglutamate synthase, mito...   517   e-171
ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichoc...   529   e-171
ref|XP_003519364.1| PREDICTED: folylpolyglutamate synthase, mito...   512   e-169

>ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricinus communis]
            gi|223536513|gb|EEF38160.1| folylpolyglutamate synthase,
            putative [Ricinus communis]
          Length = 531

 Score =  525 bits (1352), Expect(2) = e-172
 Identities = 259/362 (71%), Positives = 301/362 (83%), Gaps = 1/362 (0%)
 Frame = +1

Query: 124  SYDDAMCCLSSLITKRTRADNNNTGDAFHLLFDYLKILELEEPISQLKVIHVAGTKGKGS 303
            SY++AM  LSSLITKR+RAD +N GD F  LFDYLK+L+LE+ IS++K+IHVAGTKGKGS
Sbjct: 15   SYEEAMDALSSLITKRSRADKSNKGDRFDFLFDYLKMLDLEQAISEMKIIHVAGTKGKGS 74

Query: 304  TCAFTESILRNCGFRTGLFTSPHLIDVRERFRLDGMDICKEKFLAYFWWCWDRLKEKTSD 483
            TC FTESILRNCGFRTGLFTSPHLIDVRERFRLDG DIC+EKFLAYFWWC+DRLKEKT++
Sbjct: 75   TCTFTESILRNCGFRTGLFTSPHLIDVRERFRLDGEDICEEKFLAYFWWCYDRLKEKTTE 134

Query: 484  DIPMPSYFRFLTLLAFKIFAAEQVDVAILEVGLGGKFDATNVVKSPVVCGIASLGYDHME 663
            +IPMP+YFRFL LLAFKIFAAEQVDVAILEVGLGGKFDATNVV++P+VCGI+SLGYDHME
Sbjct: 135  EIPMPTYFRFLALLAFKIFAAEQVDVAILEVGLGGKFDATNVVQTPIVCGISSLGYDHME 194

Query: 664  ILGNTLAQIAGEKAGIFKHGVPAFTVPQPDEAMNVLVEKSSKLDVHLKIAAPLDSSXXXX 843
            ILGNTL +IAGEKAGIFKH +PAFTVPQ DEAM+VL +K+SKLDV L++A PLD S    
Sbjct: 195  ILGNTLGEIAGEKAGIFKHRIPAFTVPQLDEAMHVLEDKASKLDVPLQVAPPLDPSLLNG 254

Query: 844  XXXXXXXXXQYGNAGLALALCSTWLQRTGHAKIKYLENNT-LPEKFIKGLTTAGLQGRGQ 1020
                     QY NAGLA+AL S WLQRTGH +  YLE ++ LPE FI+GLTTA LQGR Q
Sbjct: 255  LKLGLEGEHQYLNAGLAIALSSKWLQRTGHVEFSYLEQSSPLPEHFIRGLTTACLQGRAQ 314

Query: 1021 VVSDEIIETERHGDLVFYLDGAHSPESMDVCANWFSRILKEDYRHISLNTHEHNQSRTSY 1200
            +V D  + TE +GDLVFYLDGAHSPESM+ CA WFS  +K+D +  +LN+  H     +Y
Sbjct: 315  IVPDRCVNTETNGDLVFYLDGAHSPESMEACARWFSLAIKDDNQQHTLNSPPHR----NY 370

Query: 1201 ES 1206
            ES
Sbjct: 371  ES 372



 Score =  107 bits (267), Expect(2) = e-172
 Identities = 52/76 (68%), Positives = 56/76 (73%)
 Frame = +3

Query: 1299 QILLFNCMSVRDPNLLLPHLMSTSAKHGVYFKKAFFVPSMSVYNKVGKAVDSPKDAHVDI 1478
            QILLFNCMSVRDP LLLP LM T A  GVYFKKA FVP+ SVY KVG     P D  VD+
Sbjct: 381  QILLFNCMSVRDPQLLLPRLMKTCASQGVYFKKALFVPNTSVYYKVGSQALPPVDPQVDL 440

Query: 1479 SWQLTLQRVWENLKSG 1526
            SWQ TLQR+WE+L  G
Sbjct: 441  SWQFTLQRLWESLVRG 456


>emb|CBI31610.3| unnamed protein product [Vitis vinifera]
          Length = 618

 Score =  520 bits (1339), Expect(2) = e-171
 Identities = 255/382 (66%), Positives = 303/382 (79%), Gaps = 1/382 (0%)
 Frame = +1

Query: 109  ESRTMSYDDAMCCLSSLITKRTRADNNNTGDAFHLLFDYLKILELEEPISQLKVIHVAGT 288
            ES    Y + +  LSSLITKR+R D +N    F +LFDYLK+L+L+EPIS++K+IHVAGT
Sbjct: 91   ESTVSPYQEVLDALSSLITKRSRGDKSNKRVLFDVLFDYLKMLDLDEPISKMKIIHVAGT 150

Query: 289  KGKGSTCAFTESILRNCGFRTGLFTSPHLIDVRERFRLDGMDICKEKFLAYFWWCWDRLK 468
            KGKGSTC FTESILRNCGFRTGLFTSPHLIDVRERFRLDG++IC+EKFLAYFWWCWDRLK
Sbjct: 151  KGKGSTCTFTESILRNCGFRTGLFTSPHLIDVRERFRLDGVEICEEKFLAYFWWCWDRLK 210

Query: 469  EKTSDDIPMPSYFRFLTLLAFKIFAAEQVDVAILEVGLGGKFDATNVVKSPVVCGIASLG 648
            EKT +D+PMP++F F+ LLAFKIFAAEQVDV ILEVGLGG+ DATNVV+ P+VCGI SLG
Sbjct: 211  EKTDEDVPMPTFFHFIALLAFKIFAAEQVDVVILEVGLGGRLDATNVVQEPIVCGITSLG 270

Query: 649  YDHMEILGNTLAQIAGEKAGIFKHGVPAFTVPQPDEAMNVLVEKSSKLDVHLKIAAPLDS 828
            YDHMEILG+TL +IAGEKAGIFK GVPAFTVPQP+EAM VL  K+S+LDV L++  P+D+
Sbjct: 271  YDHMEILGHTLGEIAGEKAGIFKRGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDA 330

Query: 829  SXXXXXXXXXXXXXQYGNAGLALALCSTWLQRTGHAKIKYLE-NNTLPEKFIKGLTTAGL 1005
            S             QY NAGLA+ALCSTWLQRT H +I YLE  ++LPE+FIKGLTTA L
Sbjct: 331  SLLNGLKLGLEGEHQYLNAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASL 390

Query: 1006 QGRGQVVSDEIIETERHGDLVFYLDGAHSPESMDVCANWFSRILKEDYRHISLNTHEHNQ 1185
            QGR Q+V D  I  E  GDLVFYLDGAHSPESM++CA WFS  +KED +  +LN    + 
Sbjct: 391  QGRAQIVPDRCINIESPGDLVFYLDGAHSPESMEICARWFSLAIKEDDQQPTLNYQPQDD 450

Query: 1186 SRTSYESVPIDHNYTSKKKSEQ 1251
              +S E V  D++ TS+K + Q
Sbjct: 451  VGSSRELVQKDYHGTSRKNTTQ 472



 Score =  110 bits (276), Expect(2) = e-171
 Identities = 52/76 (68%), Positives = 62/76 (81%)
 Frame = +3

Query: 1299 QILLFNCMSVRDPNLLLPHLMSTSAKHGVYFKKAFFVPSMSVYNKVGKAVDSPKDAHVDI 1478
            QILLFNCMSVRDP+LLLPHLM+T A HGV+FK+A FVP++SVYN+VG       D  VD+
Sbjct: 472  QILLFNCMSVRDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLTDLQVDL 531

Query: 1479 SWQLTLQRVWENLKSG 1526
            SWQLTLQR+WE+L  G
Sbjct: 532  SWQLTLQRLWESLIHG 547


>ref|XP_003544172.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Glycine
            max]
          Length = 548

 Score =  517 bits (1332), Expect(2) = e-171
 Identities = 252/362 (69%), Positives = 302/362 (83%), Gaps = 1/362 (0%)
 Frame = +1

Query: 127  YDDAMCCLSSLITKRTRADNNNTGDAFHLLFDYLKILELEEPISQLKVIHVAGTKGKGST 306
            Y++AM  LSSLIT+RTRAD++N GD F LL+DYLK+L+LEEPI+ +K+IHVAGTKGKGST
Sbjct: 19   YEEAMEALSSLITRRTRADDSNVGDQFSLLYDYLKMLDLEEPIANMKIIHVAGTKGKGST 78

Query: 307  CAFTESILRNCGFRTGLFTSPHLIDVRERFRLDGMDICKEKFLAYFWWCWDRLKEKTSDD 486
            C FTESILRNCGFRTGLFTSPHLID+RERFRLDGM+IC+EKFLAYFWWC+DRLKEKT D+
Sbjct: 79   CTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGMEICEEKFLAYFWWCYDRLKEKTDDN 138

Query: 487  IPMPSYFRFLTLLAFKIFAAEQVDVAILEVGLGGKFDATNVVKSPVVCGIASLGYDHMEI 666
            IPMP+YFRFL L+AFKIFAAEQVDVAI+EVGLGGK+DATNVV+SP+VCGI SLGYDHMEI
Sbjct: 139  IPMPTYFRFLALVAFKIFAAEQVDVAIMEVGLGGKYDATNVVQSPIVCGITSLGYDHMEI 198

Query: 667  LGNTLAQIAGEKAGIFKHGVPAFTVPQPDEAMNVLVEKSSKLDVHLKIAAPLDSSXXXXX 846
            LGNTL +IAGEKAGIFK+ +PAFTVPQPDEAM+VL EK+S L+V L++  PLD+      
Sbjct: 199  LGNTLGEIAGEKAGIFKNRIPAFTVPQPDEAMHVLEEKASLLNVPLQVVTPLDAKLLNGL 258

Query: 847  XXXXXXXXQYGNAGLALALCSTWLQRTGHAKIKYLE-NNTLPEKFIKGLTTAGLQGRGQV 1023
                    QY NAGLA+ALC TWL+RTGH    Y+E  +TLPE+F+KGLT+A LQGR Q+
Sbjct: 259  RLGLEGEHQYLNAGLAIALCFTWLKRTGHVGDIYMEQTDTLPEQFVKGLTSASLQGRAQI 318

Query: 1024 VSDEIIETERHGDLVFYLDGAHSPESMDVCANWFSRILKEDYRHISLNTHEHNQSRTSYE 1203
            V D+ I++ER  +LVF+LDGAHSPESM+VCA WFS  +KE     +L   + N S+ S+E
Sbjct: 319  VPDQHIKSERSNELVFFLDGAHSPESMEVCARWFSLAIKEYNPDQTLFHQQLNNSKLSHE 378

Query: 1204 SV 1209
             V
Sbjct: 379  VV 380



 Score =  113 bits (283), Expect(2) = e-171
 Identities = 52/75 (69%), Positives = 60/75 (80%)
 Frame = +3

Query: 1302 ILLFNCMSVRDPNLLLPHLMSTSAKHGVYFKKAFFVPSMSVYNKVGKAVDSPKDAHVDIS 1481
            ILLFNC++VRDP LLLP LM T A HGVYFKKA FVPS+SV+NKVG    +P D+ VD+S
Sbjct: 397  ILLFNCLTVRDPQLLLPRLMKTCADHGVYFKKALFVPSLSVFNKVGPQAFTPTDSKVDLS 456

Query: 1482 WQLTLQRVWENLKSG 1526
            WQ +LQRVWENL  G
Sbjct: 457  WQFSLQRVWENLMQG 471


>ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222851012|gb|EEE88559.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 539

 Score =  529 bits (1362), Expect(2) = e-171
 Identities = 261/376 (69%), Positives = 307/376 (81%), Gaps = 1/376 (0%)
 Frame = +1

Query: 127  YDDAMCCLSSLITKRTRADNNNTGDAFHLLFDYLKILELEEPISQLKVIHVAGTKGKGST 306
            Y++A+  LSSLITKR+RAD +N GD F +LFDYLK   LEE IS++K+IHVAGTKGKGST
Sbjct: 17   YEEALDALSSLITKRSRADKSNNGDRFDVLFDYLK---LEEAISEMKIIHVAGTKGKGST 73

Query: 307  CAFTESILRNCGFRTGLFTSPHLIDVRERFRLDGMDICKEKFLAYFWWCWDRLKEKTSDD 486
            C FTESILRNCGFRTGLFTSPHLIDVRERFRLDG DIC+EKFLAYFWWC+DRLKEK+++D
Sbjct: 74   CTFTESILRNCGFRTGLFTSPHLIDVRERFRLDGKDICEEKFLAYFWWCYDRLKEKSTED 133

Query: 487  IPMPSYFRFLTLLAFKIFAAEQVDVAILEVGLGGKFDATNVVKSPVVCGIASLGYDHMEI 666
            +PMP+YFRFL LLAFKIF+AEQVDVAILEVGLGGKFDATNVV+ P+VCG+ASLGYDHMEI
Sbjct: 134  VPMPTYFRFLALLAFKIFSAEQVDVAILEVGLGGKFDATNVVQKPIVCGVASLGYDHMEI 193

Query: 667  LGNTLAQIAGEKAGIFKHGVPAFTVPQPDEAMNVLVEKSSKLDVHLKIAAPLDSSXXXXX 846
            LGNTLAQIAGEKAGIFK G+PAFTVPQPDEAMNVL EK+S+L+VHL++A PLD+      
Sbjct: 194  LGNTLAQIAGEKAGIFKDGIPAFTVPQPDEAMNVLEEKASELNVHLQVAEPLDAKLLNGL 253

Query: 847  XXXXXXXXQYGNAGLALALCSTWLQRTGHAKIKYLEN-NTLPEKFIKGLTTAGLQGRGQV 1023
                    QY NAGLA+AL  TWLQRTGH +  ++E  ++LPE+FIKGLTTA LQGR Q+
Sbjct: 254  KLRLEGEHQYLNAGLAIALSFTWLQRTGHHEFTHMEQASSLPEQFIKGLTTASLQGRAQI 313

Query: 1024 VSDEIIETERHGDLVFYLDGAHSPESMDVCANWFSRILKEDYRHISLNTHEHNQSRTSYE 1203
            V+D+ I  E +GDLVFYLDGAHSPESM++CA WFS  +KED +  + N    N S ++ E
Sbjct: 314  VTDQYINAESYGDLVFYLDGAHSPESMEMCARWFSLAIKEDSQKRTFNYPPQNNSESTIE 373

Query: 1204 SVPIDHNYTSKKKSEQ 1251
             V   H+    K S Q
Sbjct: 374  LVQEHHDERYGKSSMQ 389



 Score =  101 bits (252), Expect(2) = e-171
 Identities = 49/73 (67%), Positives = 53/73 (72%)
 Frame = +3

Query: 1299 QILLFNCMSVRDPNLLLPHLMSTSAKHGVYFKKAFFVPSMSVYNKVGKAVDSPKDAHVDI 1478
            QILLFNCMSVRDP LLLP LM   A HGVYFKKA FV + SVY KVG     P D+  ++
Sbjct: 389  QILLFNCMSVRDPQLLLPPLMKACASHGVYFKKALFVANTSVYYKVGSHALPPTDSQFNL 448

Query: 1479 SWQLTLQRVWENL 1517
            SWQ  LQRVWENL
Sbjct: 449  SWQFALQRVWENL 461


>ref|XP_003519364.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Glycine
            max]
          Length = 542

 Score =  512 bits (1318), Expect(2) = e-169
 Identities = 253/367 (68%), Positives = 302/367 (82%), Gaps = 1/367 (0%)
 Frame = +1

Query: 127  YDDAMCCLSSLITKRTRADNNNTGDAFHLLFDYLKILELEEPISQLKVIHVAGTKGKGST 306
            Y++AM  LSSLIT+RTRAD++N GD F LL+DYLK+LELEEPI+ +KVIHVAGTKGKGST
Sbjct: 19   YEEAMEALSSLITRRTRADDSNVGDQFSLLYDYLKMLELEEPIANMKVIHVAGTKGKGST 78

Query: 307  CAFTESILRNCGFRTGLFTSPHLIDVRERFRLDGMDICKEKFLAYFWWCWDRLKEKTSDD 486
            C FTESILRNCGFRTGLFTSPHLID+RERFRLDGM+IC+EKFLAYFWWC+DRLKEKT D+
Sbjct: 79   CTFTESILRNCGFRTGLFTSPHLIDIRERFRLDGMEICEEKFLAYFWWCYDRLKEKTDDN 138

Query: 487  IPMPSYFRFLTLLAFKIFAAEQVDVAILEVGLGGKFDATNVVKSPVVCGIASLGYDHMEI 666
            IPMP+YFRFL LLAFKIFAAEQVDVAI+EVGLGGK+DATN V++P+VCGI SLGYDHMEI
Sbjct: 139  IPMPTYFRFLALLAFKIFAAEQVDVAIMEVGLGGKYDATN-VQAPIVCGITSLGYDHMEI 197

Query: 667  LGNTLAQIAGEKAGIFKHGVPAFTVPQPDEAMNVLVEKSSKLDVHLKIAAPLDSSXXXXX 846
            LGNTL +IAGEKAGIFK+ +PAFTVPQPDEAM+VL EK+S+L+V L++  PLD+      
Sbjct: 198  LGNTLGEIAGEKAGIFKNRIPAFTVPQPDEAMHVLEEKASQLNVPLQVLTPLDAKLLNGL 257

Query: 847  XXXXXXXXQYGNAGLALALCSTWLQRTGH-AKIKYLENNTLPEKFIKGLTTAGLQGRGQV 1023
                    QY NAGLA+ALC TWL+RTGH   I   + +TLPE+FIKGLT+A LQGR Q+
Sbjct: 258  RLGLEGEHQYLNAGLAIALCFTWLKRTGHVGDINLEQTDTLPEQFIKGLTSASLQGRAQI 317

Query: 1024 VSDEIIETERHGDLVFYLDGAHSPESMDVCANWFSRILKEDYRHISLNTHEHNQSRTSYE 1203
            V D+ I++ER  +LVF+LDGAHSPESM+VCA WFS  +KE     +L   + N S+  +E
Sbjct: 318  VPDQHIKSERSNELVFFLDGAHSPESMEVCARWFSLAIKEYNPDQTLFHQQLNNSKLPHE 377

Query: 1204 SVPIDHN 1224
             V   H+
Sbjct: 378  VVKSTHH 384



 Score =  113 bits (282), Expect(2) = e-169
 Identities = 52/75 (69%), Positives = 59/75 (78%)
 Frame = +3

Query: 1302 ILLFNCMSVRDPNLLLPHLMSTSAKHGVYFKKAFFVPSMSVYNKVGKAVDSPKDAHVDIS 1481
            ILLFNC++VRDP LLLP LM T A HGVYFKKA FVPS+SV+NKVG    +P D+ VD+S
Sbjct: 396  ILLFNCLTVRDPQLLLPQLMKTCADHGVYFKKALFVPSLSVFNKVGPQAFTPADSKVDLS 455

Query: 1482 WQLTLQRVWENLKSG 1526
            WQ  LQRVWENL  G
Sbjct: 456  WQFNLQRVWENLMQG 470