BLASTX nr result
ID: Lithospermum22_contig00018309
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00018309
(1571 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q70PR7.2|VINSY_RAUSE RecName: Full=Vinorine synthase gi|60594... 263 1e-67
ref|XP_002267341.1| PREDICTED: vinorine synthase [Vitis vinifera... 257 5e-66
ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|2... 245 2e-62
ref|XP_003521961.1| PREDICTED: vinorine synthase-like [Glycine max] 245 3e-62
ref|XP_003538872.1| PREDICTED: vinorine synthase-like [Glycine max] 244 3e-62
>sp|Q70PR7.2|VINSY_RAUSE RecName: Full=Vinorine synthase gi|60594431|pdb|2BGH|A Chain A,
Crystal Structure Of Vinorine Synthase
gi|60594432|pdb|2BGH|B Chain B, Crystal Structure Of
Vinorine Synthase gi|57635335|emb|CAD89104.2| vinorine
synthase [Rauvolfia serpentina]
Length = 421
Score = 263 bits (671), Expect = 1e-67
Identities = 166/425 (39%), Positives = 244/425 (57%), Gaps = 18/425 (4%)
Frame = -2
Query: 1564 MATCIEITSKEMIKPSSPTPPHLRNHKLFLMDQTMPRCYISLIFFFQYDDDSSI--SEKS 1391
MA +E S+E+I PSSPTP L+ +K+ +DQ + C+I I F+ DS++ ++ S
Sbjct: 1 MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS 60
Query: 1390 QQFKESLSRTLTLFYPLAGRIRRNNLSVDCNDEGVEVVHAFVHTQLANVLKEP-KMNELK 1214
Q K+SLS+ LT FYPLAGRI N+ SVDCND GV V A V QL+ ++ ++ +L
Sbjct: 61 QHLKQSLSKVLTHFYPLAGRINVNS-SVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 119
Query: 1213 NLVPEQAQ-----EIGCDIPLAMQINFFDCGGIAIGVCMSHEVADVKSLAIFMHAWAATC 1049
+P A E+ D+PLA++I+FF+CGG AIGV +SH++ADV SLA F++AW ATC
Sbjct: 120 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 179
Query: 1048 RGGEEIXXXXXXXXXXXPLDLSKTSF-----TTDLNLSNKEKLTAKRIVFEKEKLDKLK- 887
RG EI DL+ F T L E + KR VF+KEK+ L+
Sbjct: 180 RGETEI--------VLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALRA 231
Query: 886 EIASRVELKNPSRVEVVSASIWKYFIGQSK---NQGNMFCSYHAIDLRPRMSKPITQNAF 716
+ +S E KN SRV++V A IWK+ I ++ N F A++LR RM+ P+ A
Sbjct: 232 QASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAM 291
Query: 715 GNIVSSVVVAMLMPGDDKSFSNLVGKYKEAVKNVDGDFYLRNAEKGDYQEILDGLEKFVE 536
GNI ++++ A + DK F +L+G + +++ + D E+L G+ E
Sbjct: 292 GNI-ATLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDD---------HNHELLKGMTCLYE 341
Query: 535 LVTKEGVEMFYFSSW-RFPWYELNFGWGKPIYVCPGVYPLKNVVILMSSRCGERVEAWIN 359
L E E+ F+SW R +Y+L+FGWGKP+ C +P +N +LM +R G+ VEAW+
Sbjct: 342 L---EPQELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLP 398
Query: 358 MLESD 344
M E +
Sbjct: 399 MAEDE 403
>ref|XP_002267341.1| PREDICTED: vinorine synthase [Vitis vinifera]
gi|297738335|emb|CBI27536.3| unnamed protein product
[Vitis vinifera]
Length = 431
Score = 257 bits (657), Expect = 5e-66
Identities = 158/417 (37%), Positives = 239/417 (57%), Gaps = 14/417 (3%)
Frame = -2
Query: 1552 IEITSKEMIKPSSPTPPHLRNHKLFLMDQTMPRCYISLIFFFQYDDDSSIS--EKSQQFK 1379
+E+ S + IKPSSPTP HLR+ +L +DQ + +I +I F+ D D + E+S K
Sbjct: 6 VEVISTDTIKPSSPTPAHLRHFQLSFLDQVLSPIFIPIILFYPMDGDVKVDTIERSIWLK 65
Query: 1378 ESLSRTLTLFYPLAGRIRRNNLSVDCNDEGVEVVHAFVHTQLANVLKEPKMNELKNLVPE 1199
++LS TL FYPLAGR++ +NL +DC D+GV A V QL+ + P +L VP
Sbjct: 66 KTLSMTLVQFYPLAGRVK-DNLFIDCTDQGVPYFEAQVKCQLSEFICNPDPMQLSKFVPH 124
Query: 1198 QAQEIGCDIPLAMQINFFDCGGIAIGVCMSHEVADVKSLAIFMHAWAATCRGGEE-IXXX 1022
+I DI LA+Q+N F CGGIAIGVC+SH+VAD S+ F++ WAA R +
Sbjct: 125 ALDDI-VDIVLAIQVNIFKCGGIAIGVCISHKVADASSVVTFVNGWAAVARRDTHMVCPQ 183
Query: 1021 XXXXXXXXPLDLSKTSFTTDLNLSNKEKLTAKRIVFEKEKLDKLKEIASRVEL-----KN 857
P++LS + +T + KEK+ KR VF + L+E + +
Sbjct: 184 FGLANLFPPINLSGFNPSTGM---TKEKILTKRFVFSASSVAALREKYADQSTTEDLPRR 240
Query: 856 PSRVEVVSASIWKYFIGQSKNQGN---MFCSYHAIDLRPRMSKPITQNAFGNIVSSVVVA 686
P+R+E +SA IW F+ + + ++ HA++LR RM P+ +N+FGNI+ +
Sbjct: 241 PTRIEALSALIWSRFVAVTHGMADPKKIYTILHAVNLRTRMDPPLPENSFGNIIWFAITR 300
Query: 685 MLMPGDDKSFSN-LVGKYKEAVKNVDGDFYLRNAEKGDYQEILDGLEKFVELVTKEGVEM 509
+ G + + LV + +EA++ +DGD Y++ +G + L+ L++ E VTK +E
Sbjct: 301 PCIDGMKEGKRHALVNQMREAIEKIDGD-YIKKLPEG--IKHLNFLKERAERVTKGEIES 357
Query: 508 FYFSSW-RFPWYELNFGWGKPIYVCPGVYPLKNVVILM-SSRCGERVEAWINMLESD 344
F F+S+ RFP YE +FGWGKP +V G P KN+VI + SS +EAWIN++E D
Sbjct: 358 FRFTSFCRFPVYEADFGWGKPKWVGSGSLPFKNLVIFIDSSGTNGGIEAWINLVEED 414
>ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|222864679|gb|EEF01810.1|
predicted protein [Populus trichocarpa]
Length = 430
Score = 245 bits (626), Expect = 2e-62
Identities = 151/415 (36%), Positives = 230/415 (55%), Gaps = 12/415 (2%)
Frame = -2
Query: 1552 IEITSKEMIKPSSPTPPHLRNHKLFLMDQTMPRCYISLIFFFQYDDDSSIS--EKSQQFK 1379
IE+ S E+IKPSSPTP HL +++L +DQ P Y L+ F+ D D I+ EK Q K
Sbjct: 5 IEVISNEIIKPSSPTPDHLCHYQLSFLDQISPPTYNPLLLFYPADGDVKINNIEKPNQLK 64
Query: 1378 ESLSRTLTLFYPLAGRIRRNNLSVDCNDEGVEVVHAFVHTQLANVLKEPKMNELKNLVPE 1199
+SLS L L+YPLAGRI+ +NL V+CNDEG+ A V +L V++ P+ +EL L+P
Sbjct: 65 QSLSEVLNLYYPLAGRIK-DNLFVECNDEGIPFFQAEVKCRLPQVVENPEPSELNKLIPF 123
Query: 1198 QAQEIGCDIPLAMQINFFDCGGIAIGVCMSHEVADVKSLAIFMHAWAATCRGGEEIXXXX 1019
+ ++PL +Q N F+CGGI IG+C+SH+V D SL F+ WAAT RG +
Sbjct: 124 ALDDAE-ELPLGIQYNIFECGGIVIGLCISHKVGDASSLFTFIKYWAATARGEADHISRP 182
Query: 1018 XXXXXXXPLDLSKTSFTTDLNLSNKEKLTAKRIVFEKEKLDKLKEIASRV--ELKN---P 854
++ + F ++ KE + KR VF ++ LKE S L+N P
Sbjct: 183 EFISATLFPPINISGFKPATGIT-KEDVVTKRFVFRSSSIELLKEKCSPASGSLENQRPP 241
Query: 853 SRVEVVSASIWKYFIGQSKNQG---NMFCSYHAIDLRPRMSKPITQNAFGNIVSSVVVAM 683
SRVE +S IW+ F SK + ++ HA++LR RM P+ + +FGN +A
Sbjct: 242 SRVEALSVFIWQRFTAASKVESRPERIYSMVHAVNLRSRMEPPLPEYSFGNYCR---IAF 298
Query: 682 LMPGDD--KSFSNLVGKYKEAVKNVDGDFYLRNAEKGDYQEILDGLEKFVELVTKEGVEM 509
+P D + NL + ++++ VD + Y++ +KG+ + + E+ + E V +
Sbjct: 299 TIPSIDTGEENYNLASQIRDSIGKVDKE-YVKKLQKGN-EHLGFMKEQAARFLRGEVVPL 356
Query: 508 FYFSSWRFPWYELNFGWGKPIYVCPGVYPLKNVVILMSSRCGERVEAWINMLESD 344
+ S RFP YE +FGW KPI+V KN+V+ M + G+ +EA +++ E D
Sbjct: 357 NFTSLCRFPLYEADFGWAKPIWVGSPSLTFKNLVVFMDTASGDGIEALVHLKEED 411
>ref|XP_003521961.1| PREDICTED: vinorine synthase-like [Glycine max]
Length = 433
Score = 245 bits (625), Expect = 3e-62
Identities = 145/421 (34%), Positives = 240/421 (57%), Gaps = 14/421 (3%)
Frame = -2
Query: 1564 MATCIEITSKEMIKPSSPTPPHLRNHKLFLMDQTMPRCYISLIFFFQYDDDSSISEKSQQ 1385
MA +EI SK+ IKPSSPTP HL++ KL L+DQ P Y+ ++ F+ + DD S +
Sbjct: 1 MAVKVEIVSKDTIKPSSPTPNHLQHFKLSLLDQLAPPFYVPILLFYSFSDD-DFKTISHK 59
Query: 1384 FKESLSRTLTLFYPLAGRIRRNNLSVDCNDEGVEVVHAFVHTQLANVLKEPKMNELKNLV 1205
K SLS+ LTL++P G +R N+ +V+CNDEG+ + V +L+NV+K P ++E+ L
Sbjct: 60 LKASLSQVLTLYHPFCGTLRGNS-AVECNDEGILYTESRVSVELSNVVKNPHLHEINELF 118
Query: 1204 P----EQAQEI--GCDIPLAMQINFFDCGGIAIGVCMSHEVADVKSLAIFMHAWAATCRG 1043
P A+E G ++ +A+Q+N F CGG+A+GVC SH++AD + A F+ AWAAT R
Sbjct: 119 PFDPYNPARETLEGRNM-MAVQLNQFKCGGVALGVCFSHKIADASTAASFLSAWAATSR- 176
Query: 1042 GEEIXXXXXXXXXXXPLDLSKTSFTTDL--NLSNKEKLTAKRIVFEKEKLDKLKEIASRV 869
E+ L + D+ + + + KR VF + KL++
Sbjct: 177 KEDNNKVVPPQMEEGALLFPPRNIEMDMTRGMVGDKDIVTKRFVFNDSNISKLRQKMGCF 236
Query: 868 ELKNPSRVEVVSASIWKYFIGQSKN---QGNMFCSY--HAIDLRPRMSKPITQNAFGNIV 704
NP+RVE V+A IWK + +K +G S HA+++R R+ ++ GN+
Sbjct: 237 NF-NPTRVEAVTALIWKSSLEAAKERSAEGRFPASMISHAVNIRHRIMASSKHHSIGNLW 295
Query: 703 SSVVVAMLMPGDDKSFSNLVGKYKEAVKNVDGDFYLRNAEKGDYQEILDGLEKFVELVTK 524
V ++ ++ +L + ++ + VDG+ Y+ + ++ ++++ L++ + ++
Sbjct: 296 QQAVSQLVEVEEEMGLCDLAERVRKTTREVDGN-YVAKLQGLEFYKVIESLKEARIMASE 354
Query: 523 EGVEMFYFSSW-RFPWYELNFGWGKPIYVCPGVYPLKNVVILMSSRCGERVEAWINMLES 347
+GV + FSSW RF +YE++FGWGKP YV P+KNVVILM ++ G+ +EAW+ + S
Sbjct: 355 KGVPCYSFSSWVRFGFYEVDFGWGKPTYVRTIGVPIKNVVILMGTKDGDGLEAWVTLTTS 414
Query: 346 D 344
+
Sbjct: 415 N 415
>ref|XP_003538872.1| PREDICTED: vinorine synthase-like [Glycine max]
Length = 433
Score = 244 bits (624), Expect = 3e-62
Identities = 154/418 (36%), Positives = 232/418 (55%), Gaps = 15/418 (3%)
Frame = -2
Query: 1552 IEITSKEMIKPSSPTPPHLRNHKLFLMDQTMPRCYISLIFFFQYDDDSS------ISEKS 1391
+E+ SKE++KPSSPTP HLR++ L L+D P+ S+++FF ++ S+ S
Sbjct: 5 VEVISKELVKPSSPTPNHLRHYNLSLLDHLTPQLNNSMVYFFAANNVSNQFLNTCTENAS 64
Query: 1390 QQFKESLSRTLTLFYPLAGRIRRNNLSVDCNDEGVEVVHAFVHTQLANVLKEPKMNELKN 1211
K+SLS LT +YPLAGR+ + ++CNDEG + A V +L +V++ P NE+ N
Sbjct: 65 NHLKKSLSEALTHYYPLAGRLV-DKAFIECNDEGALYLEAKVRCKLNDVVESPIPNEVTN 123
Query: 1210 LVPEQAQEIGCDIPLAMQINFFDCGGIAIGVCMSHEVADVKSLAIFMHAWAATCRGGEEI 1031
L+P +I D PL +Q+N F+CGGIAIG CMSH++AD S +F+ WAA R EI
Sbjct: 124 LLPFGMDDI-VDTPLGVQLNVFECGGIAIGACMSHKIADAMSFFVFIQTWAAIARDYNEI 182
Query: 1030 XXXXXXXXXXXPLDLSKTSFTTDLNLSNKEKLTAKRI-VFEKEKLDKLK-EIASRVEL-K 860
P D+ D N + + T RI VF+ +D LK + A ++ L K
Sbjct: 183 KTHFVSASLFPPRDIP----WYDPNKTITKPNTVSRIFVFDASVIDGLKAKYAEKMALQK 238
Query: 859 NPSRVEVVSASIWKYFIGQSK----NQGNMFCSYHAIDLRPRMSKPITQNAFGNIVSSVV 692
PSRVE +S IW F+ ++ + H ++LR RM P+ +AFGN +V
Sbjct: 239 PPSRVEALSTFIWTRFMASTQVAASESSKFYVVAHTVNLRSRMDPPLPAHAFGNYYRAVK 298
Query: 691 VAMLMPGDDKSFS-NLVGKYKEAVKNVDGDFYLRNAEKGDYQEIL-DGLEKFVELVTKEG 518
+ DDK LV K +E ++ +D ++ L+ E +Y L + L +F E + E
Sbjct: 299 AFPSL--DDKGECYELVEKLREEIRKIDNEYILKLQEGSEYLSSLREDLRRF-ENIKGEI 355
Query: 517 VEMFYFSSWRFPWYELNFGWGKPIYVCPGVYPLKNVVILMSSRCGERVEAWINMLESD 344
V + + RFP Y+ +FGWGKPI+ CP + +KNVV+ ++ G +EA I+M+E D
Sbjct: 356 VPFTFTALCRFPVYDADFGWGKPIWACPPAWKVKNVVVFTDTKFGGGIEAHISMMEED 413