BLASTX nr result
ID: Lithospermum22_contig00017961
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00017961
(1449 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum] 639 0.0
gb|ADN92997.2| 12-oxophytodienoate reductase OPR3 [Ipomoea nil] 637 e-180
ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lyc... 636 e-180
pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-... 636 e-180
pdb|2HS8|A Chain A, Crystal Structure Of The Y364f Mutant Of 12-... 634 e-179
>gb|AER51971.1| 12-oxophytodienoate reductase 3 [Solanum tuberosum]
Length = 400
Score = 639 bits (1648), Expect = 0.0
Identities = 305/400 (76%), Positives = 351/400 (87%), Gaps = 1/400 (0%)
Frame = -3
Query: 1288 LKNMPKKSSDQGKQSLFSPYKMGRFDLSHRVVLAPMTRCRAIDSIPQAALAEYYAQRATR 1109
+ N S+ G LFSPYKMG+F+LSHR+VLAPMTRCRA+++IPQAAL EYY QRAT
Sbjct: 1 MANTTSSSAQDGNNPLFSPYKMGKFNLSHRIVLAPMTRCRALNNIPQAALGEYYEQRATA 60
Query: 1108 GGFLITEGTMISPTSAGFPHVPGIFNTEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQ 929
GGFLITEGTMISPTSAGFPHVPGIF EQVE WKK+V+ VHAKGA+IFCQLWHVGRASH+
Sbjct: 61 GGFLITEGTMISPTSAGFPHVPGIFTKEQVEEWKKIVDVVHAKGAVIFCQLWHVGRASHE 120
Query: 928 VYQPGGNAPISSTDKPVSKRWRILMPDASHAIYPKPRPLTIHEIPEVVEHYRQAALNAIE 749
VYQP G APISST+ P+SKRWRILMPD +H IYPKPR + +EI +VVE YR++ALNAIE
Sbjct: 121 VYQPAGAAPISSTEMPISKRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIE 180
Query: 748 AGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLSNRCRFLIKVVRSIVESIGADRVSV 569
AGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSL+NRC+F+ +VV+++V +IGADRV V
Sbjct: 181 AGFDGIEIHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGV 240
Query: 568 RISPAIDHLDAMDSDPLSLGLAVIDRLNDLQAEKGAKLAYLHMTQPRYTAYGQTEAGRHG 389
R+SPAIDHLDAMDS+PLSLGLAV++RLN +Q G+KLAYLH+TQPRY AYGQTEAGR G
Sbjct: 241 RVSPAIDHLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLG 300
Query: 388 SEEEEVRMMRTCRQAYQGTFMASGGFTRQLGIEAVAEGDADLVSYGRVFIANPDLVMRLK 209
SEEEE R+MRT R AYQGTF+ SGG+TR+LGIEAVA+GDADLVSYGR+FI+NPDLVMR+K
Sbjct: 301 SEEEEARLMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIK 360
Query: 208 IDAPLNKYVRGTFYTYDPVVGYTDYPFLSEDPSKIP-SRL 92
++APLNKY R TFYT DPVVGYTDYPFL + S P SRL
Sbjct: 361 LNAPLNKYNRKTFYTQDPVVGYTDYPFLQGNGSNGPLSRL 400
>gb|ADN92997.2| 12-oxophytodienoate reductase OPR3 [Ipomoea nil]
Length = 400
Score = 637 bits (1642), Expect = e-180
Identities = 305/385 (79%), Positives = 346/385 (89%), Gaps = 1/385 (0%)
Frame = -3
Query: 1267 SSDQGKQ-SLFSPYKMGRFDLSHRVVLAPMTRCRAIDSIPQAALAEYYAQRATRGGFLIT 1091
S DQG SLFSPYKMGRF+LSHRVVLAP TRCRAI+ IPQAALAEYY+QRAT+GGFLIT
Sbjct: 6 SLDQGSTPSLFSPYKMGRFNLSHRVVLAPCTRCRAINGIPQAALAEYYSQRATQGGFLIT 65
Query: 1090 EGTMISPTSAGFPHVPGIFNTEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQPGG 911
EGTMI PTSAGFPHVPGIF EQVEAWKKVV+AVHAKGAIIFCQLWHVGRASHQVYQPGG
Sbjct: 66 EGTMIDPTSAGFPHVPGIFTKEQVEAWKKVVDAVHAKGAIIFCQLWHVGRASHQVYQPGG 125
Query: 910 NAPISSTDKPVSKRWRILMPDASHAIYPKPRPLTIHEIPEVVEHYRQAALNAIEAGFDGI 731
+APISSTDKP+SKRWRILMPD SH IYPKPR + + +I VVE+YR AA+NAIEAGFDG+
Sbjct: 126 SAPISSTDKPISKRWRILMPDGSHGIYPKPRAVGVDDIANVVEYYRIAAINAIEAGFDGV 185
Query: 730 EIHGAHGYLIDQFLKDGINDRTDEYGGSLSNRCRFLIKVVRSIVESIGADRVSVRISPAI 551
EIHGAHGYLIDQFLKDGIN+R DEYGGSL+NRC+F+++VV+++ +IGADR+ VRISPAI
Sbjct: 186 EIHGAHGYLIDQFLKDGINNRKDEYGGSLANRCKFIMQVVQAVATAIGADRLGVRISPAI 245
Query: 550 DHLDAMDSDPLSLGLAVIDRLNDLQAEKGAKLAYLHMTQPRYTAYGQTEAGRHGSEEEEV 371
DHLDAMDS PLSLGLAV++RLN LQ + KLAYLH+TQPRY AYGQTE+G+ GSEEEE
Sbjct: 246 DHLDAMDSSPLSLGLAVVERLNKLQVDIRTKLAYLHVTQPRYVAYGQTESGKQGSEEEEA 305
Query: 370 RMMRTCRQAYQGTFMASGGFTRQLGIEAVAEGDADLVSYGRVFIANPDLVMRLKIDAPLN 191
R+M T R YQGTF+ SGG+TRQLGIEA+A+GDADLVSYGR+FIANPDLV+R K+DAPLN
Sbjct: 306 RLMLTLRNTYQGTFICSGGYTRQLGIEAIAQGDADLVSYGRLFIANPDLVLRFKLDAPLN 365
Query: 190 KYVRGTFYTYDPVVGYTDYPFLSED 116
KY R TFYT DPVVGYTDYPFL+++
Sbjct: 366 KYNRKTFYTQDPVVGYTDYPFLNKE 390
>ref|NP_001233873.1| 12-oxophytodienoate reductase 3 [Solanum lycopersicum]
gi|62900706|sp|Q9FEW9.1|OPR3_SOLLC RecName:
Full=12-oxophytodienoate reductase 3; AltName:
Full=12-oxophytodienoate-10,11-reductase 3;
Short=OPDA-reductase 3; AltName: Full=LeOPR3
gi|12056507|emb|CAC21424.1| 12-oxophytodienoate reductase
3 [Solanum lycopersicum]
Length = 396
Score = 636 bits (1640), Expect = e-180
Identities = 304/393 (77%), Positives = 348/393 (88%), Gaps = 1/393 (0%)
Frame = -3
Query: 1267 SSDQGKQSLFSPYKMGRFDLSHRVVLAPMTRCRAIDSIPQAALAEYYAQRATRGGFLITE 1088
S+ G LFSPYKMG+F+LSHRVVLAPMTRCRA+++IPQAAL EYY QRAT GGFLITE
Sbjct: 4 SAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITE 63
Query: 1087 GTMISPTSAGFPHVPGIFNTEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQPGGN 908
GTMISPTSAGFPHVPGIF EQV WKK+V+ VHAKGA+IFCQLWHVGRASH+VYQP G
Sbjct: 64 GTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGA 123
Query: 907 APISSTDKPVSKRWRILMPDASHAIYPKPRPLTIHEIPEVVEHYRQAALNAIEAGFDGIE 728
APISST+KP+S RWRILMPD +H IYPKPR + +EI +VVE YR++ALNAIEAGFDGIE
Sbjct: 124 APISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIE 183
Query: 727 IHGAHGYLIDQFLKDGINDRTDEYGGSLSNRCRFLIKVVRSIVESIGADRVSVRISPAID 548
IHGAHGYLIDQFLKDGINDRTDEYGGSL+NRC+F+ +VV+++V +IGADRV VR+SPAID
Sbjct: 184 IHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAID 243
Query: 547 HLDAMDSDPLSLGLAVIDRLNDLQAEKGAKLAYLHMTQPRYTAYGQTEAGRHGSEEEEVR 368
HLDAMDS+PLSLGLAV++RLN +Q G+KLAYLH+TQPRY AYGQTEAGR GSEEEE R
Sbjct: 244 HLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEAR 303
Query: 367 MMRTCRQAYQGTFMASGGFTRQLGIEAVAEGDADLVSYGRVFIANPDLVMRLKIDAPLNK 188
+MRT R AYQGTF+ SGG+TR+LGIEAVA+GDADLVSYGR+FI+NPDLVMR+K++APLNK
Sbjct: 304 LMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNK 363
Query: 187 YVRGTFYTYDPVVGYTDYPFLSEDPSKIP-SRL 92
Y R TFYT DPVVGYTDYPFL + S P SRL
Sbjct: 364 YNRKTFYTQDPVVGYTDYPFLQGNGSNGPLSRL 396
>pdb|2HS6|A Chain A, Crystal Structure Of The E291k Mutant Of 12-
Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|116667815|pdb|2HS6|B Chain B, Crystal Structure Of The
E291k Mutant Of 12- Oxophytodienoate Reductase 3 (Opr3)
From Tomato gi|116667818|pdb|2HSA|B Chain B, Crystal
Structure Of 12-Oxophytodienoate Reductase 3 (Opr3) From
Tomato gi|116667819|pdb|2HSA|A Chain A, Crystal Structure
Of 12-Oxophytodienoate Reductase 3 (Opr3) From Tomato
gi|256599757|pdb|3HGS|A Chain A, Crystal Structure Of
Tomato Opr3 In Complex With Phb gi|256599758|pdb|3HGS|B
Chain B, Crystal Structure Of Tomato Opr3 In Complex With
Phb
Length = 402
Score = 636 bits (1640), Expect = e-180
Identities = 304/393 (77%), Positives = 348/393 (88%), Gaps = 1/393 (0%)
Frame = -3
Query: 1267 SSDQGKQSLFSPYKMGRFDLSHRVVLAPMTRCRAIDSIPQAALAEYYAQRATRGGFLITE 1088
S+ G LFSPYKMG+F+LSHRVVLAPMTRCRA+++IPQAAL EYY QRAT GGFLITE
Sbjct: 10 SAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITE 69
Query: 1087 GTMISPTSAGFPHVPGIFNTEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQPGGN 908
GTMISPTSAGFPHVPGIF EQV WKK+V+ VHAKGA+IFCQLWHVGRASH+VYQP G
Sbjct: 70 GTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGA 129
Query: 907 APISSTDKPVSKRWRILMPDASHAIYPKPRPLTIHEIPEVVEHYRQAALNAIEAGFDGIE 728
APISST+KP+S RWRILMPD +H IYPKPR + +EI +VVE YR++ALNAIEAGFDGIE
Sbjct: 130 APISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIE 189
Query: 727 IHGAHGYLIDQFLKDGINDRTDEYGGSLSNRCRFLIKVVRSIVESIGADRVSVRISPAID 548
IHGAHGYLIDQFLKDGINDRTDEYGGSL+NRC+F+ +VV+++V +IGADRV VR+SPAID
Sbjct: 190 IHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAID 249
Query: 547 HLDAMDSDPLSLGLAVIDRLNDLQAEKGAKLAYLHMTQPRYTAYGQTEAGRHGSEEEEVR 368
HLDAMDS+PLSLGLAV++RLN +Q G+KLAYLH+TQPRY AYGQTEAGR GSEEEE R
Sbjct: 250 HLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEAR 309
Query: 367 MMRTCRQAYQGTFMASGGFTRQLGIEAVAEGDADLVSYGRVFIANPDLVMRLKIDAPLNK 188
+MRT R AYQGTF+ SGG+TR+LGIEAVA+GDADLVSYGR+FI+NPDLVMR+K++APLNK
Sbjct: 310 LMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNK 369
Query: 187 YVRGTFYTYDPVVGYTDYPFLSEDPSKIP-SRL 92
Y R TFYT DPVVGYTDYPFL + S P SRL
Sbjct: 370 YNRKTFYTQDPVVGYTDYPFLQGNGSNGPLSRL 402
>pdb|2HS8|A Chain A, Crystal Structure Of The Y364f Mutant Of 12-
Oxophytodienoate Reductase 3 From Tomato
gi|116667817|pdb|2HS8|B Chain B, Crystal Structure Of The
Y364f Mutant Of 12- Oxophytodienoate Reductase 3 From
Tomato
Length = 402
Score = 634 bits (1636), Expect = e-179
Identities = 303/393 (77%), Positives = 348/393 (88%), Gaps = 1/393 (0%)
Frame = -3
Query: 1267 SSDQGKQSLFSPYKMGRFDLSHRVVLAPMTRCRAIDSIPQAALAEYYAQRATRGGFLITE 1088
S+ G LFSPYKMG+F+LSHRVVLAPMTRCRA+++IPQAAL EYY QRAT GGFLITE
Sbjct: 10 SAQDGNNPLFSPYKMGKFNLSHRVVLAPMTRCRALNNIPQAALGEYYEQRATAGGFLITE 69
Query: 1087 GTMISPTSAGFPHVPGIFNTEQVEAWKKVVEAVHAKGAIIFCQLWHVGRASHQVYQPGGN 908
GTMISPTSAGFPHVPGIF EQV WKK+V+ VHAKGA+IFCQLWHVGRASH+VYQP G
Sbjct: 70 GTMISPTSAGFPHVPGIFTKEQVREWKKIVDVVHAKGAVIFCQLWHVGRASHEVYQPAGA 129
Query: 907 APISSTDKPVSKRWRILMPDASHAIYPKPRPLTIHEIPEVVEHYRQAALNAIEAGFDGIE 728
APISST+KP+S RWRILMPD +H IYPKPR + +EI +VVE YR++ALNAIEAGFDGIE
Sbjct: 130 APISSTEKPISNRWRILMPDGTHGIYPKPRAIGTYEISQVVEDYRRSALNAIEAGFDGIE 189
Query: 727 IHGAHGYLIDQFLKDGINDRTDEYGGSLSNRCRFLIKVVRSIVESIGADRVSVRISPAID 548
IHGAHGYLIDQFLKDGINDRTDEYGGSL+NRC+F+ +VV+++V +IGADRV VR+SPAID
Sbjct: 190 IHGAHGYLIDQFLKDGINDRTDEYGGSLANRCKFITQVVQAVVSAIGADRVGVRVSPAID 249
Query: 547 HLDAMDSDPLSLGLAVIDRLNDLQAEKGAKLAYLHMTQPRYTAYGQTEAGRHGSEEEEVR 368
HLDAMDS+PLSLGLAV++RLN +Q G+KLAYLH+TQPRY AYGQTEAGR GSEEEE R
Sbjct: 250 HLDAMDSNPLSLGLAVVERLNKIQLHSGSKLAYLHVTQPRYVAYGQTEAGRLGSEEEEAR 309
Query: 367 MMRTCRQAYQGTFMASGGFTRQLGIEAVAEGDADLVSYGRVFIANPDLVMRLKIDAPLNK 188
+MRT R AYQGTF+ SGG+TR+LGIEAVA+GDADLVSYGR+FI+NPDLVMR+K++APLNK
Sbjct: 310 LMRTLRNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNK 369
Query: 187 YVRGTFYTYDPVVGYTDYPFLSEDPSKIP-SRL 92
+ R TFYT DPVVGYTDYPFL + S P SRL
Sbjct: 370 FNRKTFYTQDPVVGYTDYPFLQGNGSNGPLSRL 402