BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00017699
         (1733 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272864.1| PREDICTED: phospholipase D p1-like [Vitis vi...   959   0.0  
ref|XP_002516974.1| phospholipase d zeta, putative [Ricinus comm...   947   0.0  
emb|CBI22957.3| unnamed protein product [Vitis vinifera]              947   0.0  
ref|XP_002328619.1| predicted protein [Populus trichocarpa] gi|2...   943   0.0  
ref|XP_004150090.1| PREDICTED: phospholipase D p1-like [Cucumis ...   934   0.0  

>ref|XP_002272864.1| PREDICTED: phospholipase D p1-like [Vitis vinifera]
          Length = 1098

 Score =  959 bits (2478), Expect = 0.0
 Identities = 443/575 (77%), Positives = 511/575 (88%)
 Frame = -2

Query: 1729 QLVKKASQVFYLHFALKRRAIVEEIHEKQEQVREWLQYLGILDHPTVLHXXXXXXXXXXD 1550
            +L+KKASQVF+LHFALK+R I+EEI EKQEQV+EWLQ +GI +H  V+H           
Sbjct: 80   RLIKKASQVFFLHFALKKRVIIEEIQEKQEQVKEWLQNIGIGEHTAVVHDDDEPD----- 134

Query: 1549 EEYVPLRNDESVRNRDVPSSAALPVIRPALGRQYSMSDRAKTAMQGYLNHFLGNMDIVNS 1370
            EE VPL +DESV+NRD+PSSAALP+IRPALGRQ S+SDRAK AMQGYLN FLGN+DIVNS
Sbjct: 135  EETVPLHHDESVKNRDIPSSAALPIIRPALGRQNSVSDRAKVAMQGYLNLFLGNLDIVNS 194

Query: 1369 REVCKFLDVSMLSFSPEYGPKLKEEYLLVKHLPKLPSFEEDSECCSCQWFSCCKDQWQKV 1190
            REVCKFL+VS LSFSPEYGPKLKE+Y++VKHLPK+P  ++  +CC C WFSCC D WQKV
Sbjct: 195  REVCKFLEVSKLSFSPEYGPKLKEDYVMVKHLPKIPKEDDTRKCCPCPWFSCCNDNWQKV 254

Query: 1189 WAVLKPGFLAFLIDPFDSKPVDIVVFDVLPSSEGNGENRVALAKEVKDHNPLRHYFRVSC 1010
            WAVLKPGFLA L DPF  +P+DI+VFD+LP+S+GNGE R++LAKE+K+ NPLRH  +V+C
Sbjct: 255  WAVLKPGFLALLEDPFHPQPLDIIVFDLLPASDGNGEGRLSLAKEIKERNPLRHALKVTC 314

Query: 1009 GTRMIKIRTRSKAKAKDWVSAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFVD 830
            G R I++R +S AK KDWV+AINDAGLRPPEGWCHPHRFGSFAPPRGL+EDGS AQWFVD
Sbjct: 315  GNRSIRLRAKSSAKVKDWVAAINDAGLRPPEGWCHPHRFGSFAPPRGLSEDGSLAQWFVD 374

Query: 829  GRAAFEAIALAIEDSKSEIFICGWWLCPDLYLRRPFHIHASSRLDSLLEAKAKQGVQIYI 650
            GRAAFEAIA AIE++KSEIFICGWW+CP+LYLRRPFH HASSRLD+LLEAKAKQGVQIYI
Sbjct: 375  GRAAFEAIASAIEEAKSEIFICGWWVCPELYLRRPFHSHASSRLDALLEAKAKQGVQIYI 434

Query: 649  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYFWSHHEKIVVVDHHICFI 470
            LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFS+GVY WSHHEK+V+VD+ ICFI
Sbjct: 435  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSTGVYLWSHHEKLVIVDYQICFI 494

Query: 469  GGLDLCFGRFDSIEHKVGDNPPEIWPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH 290
            GGLDLCFGR+D++EHKVGD+PP +WPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH
Sbjct: 495  GGLDLCFGRYDTLEHKVGDHPPLMWPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH 554

Query: 289  DVHCALWGPPCRDIARHFVQRWNYAKRSKAPNEQAIPLLMPQHHMVIPHYLGETAETEVE 110
            DVHCALWGPPCRD+ARHFVQRWNYAKR+KAPNEQAIPLLMPQ HMVIPHY+G + E EVE
Sbjct: 555  DVHCALWGPPCRDVARHFVQRWNYAKRNKAPNEQAIPLLMPQQHMVIPHYMGRSREMEVE 614

Query: 109  QRDGENEPMNSIQQEQETASTRSSFQDIPLLITQE 5
            +++ EN   +   ++ ++ S+RSSFQDIPLL+ QE
Sbjct: 615  KKNVENNYKDI--KKLDSFSSRSSFQDIPLLLPQE 647


>ref|XP_002516974.1| phospholipase d zeta, putative [Ricinus communis]
            gi|223544062|gb|EEF45588.1| phospholipase d zeta,
            putative [Ricinus communis]
          Length = 1117

 Score =  947 bits (2449), Expect = 0.0
 Identities = 439/576 (76%), Positives = 503/576 (87%)
 Frame = -2

Query: 1729 QLVKKASQVFYLHFALKRRAIVEEIHEKQEQVREWLQYLGILDHPTVLHXXXXXXXXXXD 1550
            QL KKA+QVFYLHFALKRRA  EEIHEKQEQV+EWLQ LGI DH  V+            
Sbjct: 93   QLSKKAAQVFYLHFALKRRAFFEEIHEKQEQVKEWLQNLGIGDHTPVVQDDDDAD----- 147

Query: 1549 EEYVPLRNDESVRNRDVPSSAALPVIRPALGRQYSMSDRAKTAMQGYLNHFLGNMDIVNS 1370
            +E + L N+ES +NR+VPS AALPVIRPALGRQ+SMSDRAK AMQ YLNHFLGN+DIVNS
Sbjct: 148  DETILLHNEESAKNRNVPSRAALPVIRPALGRQHSMSDRAKVAMQEYLNHFLGNLDIVNS 207

Query: 1369 REVCKFLDVSMLSFSPEYGPKLKEEYLLVKHLPKLPSFEEDSECCSCQWFSCCKDQWQKV 1190
            REVCKFL+VS LSFS EYGPKLKE+Y++ +HLP +P+ ++  +CC+C WFSCC D WQKV
Sbjct: 208  REVCKFLEVSKLSFSLEYGPKLKEDYVMARHLPPIPTNDDSGKCCACHWFSCCNDNWQKV 267

Query: 1189 WAVLKPGFLAFLIDPFDSKPVDIVVFDVLPSSEGNGENRVALAKEVKDHNPLRHYFRVSC 1010
            WAVLKPGFLA L DPFD+KP+DI+VFDVLP+S+G+GE R++LA E K+ NPLRH F+V+C
Sbjct: 268  WAVLKPGFLALLADPFDAKPLDIIVFDVLPASDGSGEGRISLAMETKERNPLRHAFKVTC 327

Query: 1009 GTRMIKIRTRSKAKAKDWVSAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFVD 830
            G R IK+RT++ A+ KDWV+AINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWF+D
Sbjct: 328  GVRSIKLRTKTGARVKDWVAAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFID 387

Query: 829  GRAAFEAIALAIEDSKSEIFICGWWLCPDLYLRRPFHIHASSRLDSLLEAKAKQGVQIYI 650
            G AAF+AIA +IED+KSEIFICGWWLCP+LYLRRPFH HASSRLD LLEAKAKQGVQIYI
Sbjct: 388  GMAAFDAIASSIEDAKSEIFICGWWLCPELYLRRPFHAHASSRLDDLLEAKAKQGVQIYI 447

Query: 649  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYFWSHHEKIVVVDHHICFI 470
            LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVY WSHHEK+V+VD+ ICFI
Sbjct: 448  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYLWSHHEKLVIVDYQICFI 507

Query: 469  GGLDLCFGRFDSIEHKVGDNPPEIWPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH 290
            GGLDLCFGR+D+ EH+VGD PP +WPGKDYYNPRESEPNSWEDTMKDELDR KYPRMPWH
Sbjct: 508  GGLDLCFGRYDTREHRVGDCPPFVWPGKDYYNPRESEPNSWEDTMKDELDRKKYPRMPWH 567

Query: 289  DVHCALWGPPCRDIARHFVQRWNYAKRSKAPNEQAIPLLMPQHHMVIPHYLGETAETEVE 110
            DVHCALWGPPCRD+ARHFVQRWNYAKR+KAP E+AIPLLMPQHHMVIPHY G + + EVE
Sbjct: 568  DVHCALWGPPCRDVARHFVQRWNYAKRNKAPYEEAIPLLMPQHHMVIPHYRGSSKDLEVE 627

Query: 109  QRDGENEPMNSIQQEQETASTRSSFQDIPLLITQEA 2
             ++GE++      + +++ S+RSS QDIPLL+ QEA
Sbjct: 628  TKNGEDDSKGI--KREDSFSSRSSLQDIPLLLPQEA 661


>emb|CBI22957.3| unnamed protein product [Vitis vinifera]
          Length = 1121

 Score =  947 bits (2447), Expect = 0.0
 Identities = 443/595 (74%), Positives = 511/595 (85%), Gaps = 20/595 (3%)
 Frame = -2

Query: 1729 QLVKKASQVFYLHFALKRRAIVEEIHEKQEQVREWLQYLGILDHPTVLHXXXXXXXXXXD 1550
            +L+KKASQVF+LHFALK+R I+EEI EKQEQV+EWLQ +GI +H  V+H           
Sbjct: 97   RLIKKASQVFFLHFALKKRVIIEEIQEKQEQVKEWLQNIGIGEHTAVVHDDDEPD----- 151

Query: 1549 EEYVPLRNDESVRNRDVPSSAALPVIRPALGRQYSMSDRAKTAMQGYLNHFLGNMDIVNS 1370
            EE VPL +DESV+NRD+PSSAALP+IRPALGRQ S+SDRAK AMQGYLN FLGN+DIVNS
Sbjct: 152  EETVPLHHDESVKNRDIPSSAALPIIRPALGRQNSVSDRAKVAMQGYLNLFLGNLDIVNS 211

Query: 1369 REVCKFLDVSMLSFSPEYGPKLKEEYLLVKHLPKLPSFEEDSECCSCQWFSCCKDQWQKV 1190
            REVCKFL+VS LSFSPEYGPKLKE+Y++VKHLPK+P  ++  +CC C WFSCC D WQKV
Sbjct: 212  REVCKFLEVSKLSFSPEYGPKLKEDYVMVKHLPKIPKEDDTRKCCPCPWFSCCNDNWQKV 271

Query: 1189 WAVLKPGFLAFLIDPFDSKPVDIVVFDVLPSSEGNGENRVALAKEVKDHNPLRHYFRVSC 1010
            WAVLKPGFLA L DPF  +P+DI+VFD+LP+S+GNGE R++LAKE+K+ NPLRH  +V+C
Sbjct: 272  WAVLKPGFLALLEDPFHPQPLDIIVFDLLPASDGNGEGRLSLAKEIKERNPLRHALKVTC 331

Query: 1009 GTRMIKIRTRSKAKAKDWVSAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFVD 830
            G R I++R +S AK KDWV+AINDAGLRPPEGWCHPHRFGSFAPPRGL+EDGS AQWFVD
Sbjct: 332  GNRSIRLRAKSSAKVKDWVAAINDAGLRPPEGWCHPHRFGSFAPPRGLSEDGSLAQWFVD 391

Query: 829  GRAAFEAIALAIEDSKSEIFICGWWLCPDLYLRRPFHIHASSRLDSLLEAKAKQGVQIYI 650
            GRAAFEAIA AIE++KSEIFICGWW+CP+LYLRRPFH HASSRLD+LLEAKAKQGVQIYI
Sbjct: 392  GRAAFEAIASAIEEAKSEIFICGWWVCPELYLRRPFHSHASSRLDALLEAKAKQGVQIYI 451

Query: 649  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYFWSHHEKIVVVDHHICFI 470
            LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFS+GVY WSHHEK+V+VD+ ICFI
Sbjct: 452  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSTGVYLWSHHEKLVIVDYQICFI 511

Query: 469  GGLDLCFGRFDSIEHKVGDNPPEIWPGKDYYNPR--------------------ESEPNS 350
            GGLDLCFGR+D++EHKVGD+PP +WPGKDYYNPR                    ESEPNS
Sbjct: 512  GGLDLCFGRYDTLEHKVGDHPPLMWPGKDYYNPRQFKLRLSFPCKHMGDKLHSLESEPNS 571

Query: 349  WEDTMKDELDRGKYPRMPWHDVHCALWGPPCRDIARHFVQRWNYAKRSKAPNEQAIPLLM 170
            WEDTMKDELDRGKYPRMPWHDVHCALWGPPCRD+ARHFVQRWNYAKR+KAPNEQAIPLLM
Sbjct: 572  WEDTMKDELDRGKYPRMPWHDVHCALWGPPCRDVARHFVQRWNYAKRNKAPNEQAIPLLM 631

Query: 169  PQHHMVIPHYLGETAETEVEQRDGENEPMNSIQQEQETASTRSSFQDIPLLITQE 5
            PQ HMVIPHY+G + E EVE+++ EN   +   ++ ++ S+RSSFQDIPLL+ QE
Sbjct: 632  PQQHMVIPHYMGRSREMEVEKKNVENNYKDI--KKLDSFSSRSSFQDIPLLLPQE 684


>ref|XP_002328619.1| predicted protein [Populus trichocarpa] gi|222838601|gb|EEE76966.1|
            predicted protein [Populus trichocarpa]
          Length = 1096

 Score =  943 bits (2438), Expect = 0.0
 Identities = 441/576 (76%), Positives = 503/576 (87%)
 Frame = -2

Query: 1729 QLVKKASQVFYLHFALKRRAIVEEIHEKQEQVREWLQYLGILDHPTVLHXXXXXXXXXXD 1550
            +L+KKA+QVFYLHFALK+R   EEI EKQEQV+EWLQ LGI DH  +++           
Sbjct: 73   RLLKKAAQVFYLHFALKKRVFFEEILEKQEQVKEWLQNLGIGDHTPMVNDDDDAD----- 127

Query: 1549 EEYVPLRNDESVRNRDVPSSAALPVIRPALGRQYSMSDRAKTAMQGYLNHFLGNMDIVNS 1370
            +E +PL +DES +NRDVPSSAALPVIRPALGRQ SMSDRAK  MQ YLNHFLGNMDIVNS
Sbjct: 128  DETIPLHHDESAKNRDVPSSAALPVIRPALGRQNSMSDRAKVTMQQYLNHFLGNMDIVNS 187

Query: 1369 REVCKFLDVSMLSFSPEYGPKLKEEYLLVKHLPKLPSFEEDSECCSCQWFSCCKDQWQKV 1190
            REVCKFL+VS LSFSPEYGPKLKEEY++VKHLP++   ++  +CC+C WFSCC D WQKV
Sbjct: 188  REVCKFLEVSKLSFSPEYGPKLKEEYVMVKHLPRIVKDDDSRKCCACSWFSCCNDNWQKV 247

Query: 1189 WAVLKPGFLAFLIDPFDSKPVDIVVFDVLPSSEGNGENRVALAKEVKDHNPLRHYFRVSC 1010
            WAVLKPGFLA L DPFD+K +DI+VFDVLP+S+G+GE RV+LA E+K+ NPLRH F+V+C
Sbjct: 248  WAVLKPGFLALLADPFDTKLLDIIVFDVLPASDGSGEGRVSLAAEIKERNPLRHGFKVAC 307

Query: 1009 GTRMIKIRTRSKAKAKDWVSAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFVD 830
            G R I +R+++ A+ KDWV+ INDAGLRPPEGWCHPHRF SFAPPRGL+EDGSQAQWFVD
Sbjct: 308  GNRSIDLRSKNGARVKDWVATINDAGLRPPEGWCHPHRFASFAPPRGLSEDGSQAQWFVD 367

Query: 829  GRAAFEAIALAIEDSKSEIFICGWWLCPDLYLRRPFHIHASSRLDSLLEAKAKQGVQIYI 650
            GRAAFEAIAL+IED+KSEIFICGWWLCP+LYLRRPF  HASSRLDSLLEAKAKQGVQIYI
Sbjct: 368  GRAAFEAIALSIEDAKSEIFICGWWLCPELYLRRPFRAHASSRLDSLLEAKAKQGVQIYI 427

Query: 649  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYFWSHHEKIVVVDHHICFI 470
            LLYKEVALALKINSVYSK KLLSIHENVRVLRYPDHFS+GVY WSHHEK+V+VDH ICFI
Sbjct: 428  LLYKEVALALKINSVYSKTKLLSIHENVRVLRYPDHFSTGVYLWSHHEKLVIVDHQICFI 487

Query: 469  GGLDLCFGRFDSIEHKVGDNPPEIWPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH 290
            GGLDLCFGR+D+ EH+VGD PP++WPGKDYYNPRESEPNSWED MKDELDRGKYPRMPWH
Sbjct: 488  GGLDLCFGRYDTCEHRVGDCPPQVWPGKDYYNPRESEPNSWEDMMKDELDRGKYPRMPWH 547

Query: 289  DVHCALWGPPCRDIARHFVQRWNYAKRSKAPNEQAIPLLMPQHHMVIPHYLGETAETEVE 110
            DVHCALWGPPCRD+ARHFVQRWNYAKRSKAP E+AIPLLMPQ HMVIPHY+G+  E EVE
Sbjct: 548  DVHCALWGPPCRDVARHFVQRWNYAKRSKAPYEEAIPLLMPQQHMVIPHYMGQNREMEVE 607

Query: 109  QRDGENEPMNSIQQEQETASTRSSFQDIPLLITQEA 2
             R G  + +  I++ Q++ S+RSS QDIPLL+ QEA
Sbjct: 608  -RKGIKDDVKGIKR-QDSFSSRSSLQDIPLLLPQEA 641


>ref|XP_004150090.1| PREDICTED: phospholipase D p1-like [Cucumis sativus]
          Length = 1113

 Score =  934 bits (2413), Expect = 0.0
 Identities = 435/576 (75%), Positives = 502/576 (87%)
 Frame = -2

Query: 1729 QLVKKASQVFYLHFALKRRAIVEEIHEKQEQVREWLQYLGILDHPTVLHXXXXXXXXXXD 1550
            +++KKAS VFYLHFALK+RA +EEIHEKQEQV+EWLQ LGI D   V            D
Sbjct: 81   RMLKKASHVFYLHFALKKRAFIEEIHEKQEQVKEWLQNLGIGDQTAV-----PQDEDGPD 135

Query: 1549 EEYVPLRNDESVRNRDVPSSAALPVIRPALGRQYSMSDRAKTAMQGYLNHFLGNMDIVNS 1370
            +E  PL +DES +NRDVPSSAALP+IRPAL RQ+SMSDRAKTAMQGYLNHFL NMDIVNS
Sbjct: 136  DEAEPLHHDESSKNRDVPSSAALPIIRPALLRQHSMSDRAKTAMQGYLNHFLSNMDIVNS 195

Query: 1369 REVCKFLDVSMLSFSPEYGPKLKEEYLLVKHLPKLPSFEEDSECCSCQWFSCCKDQWQKV 1190
            REVC+FL+VS LSFSPEYGPKLKE+Y++VKHLPK+P  ++  +CC C WF CC D WQKV
Sbjct: 196  REVCRFLEVSKLSFSPEYGPKLKEDYVMVKHLPKIPKQDDSRKCCLCPWFGCCNDNWQKV 255

Query: 1189 WAVLKPGFLAFLIDPFDSKPVDIVVFDVLPSSEGNGENRVALAKEVKDHNPLRHYFRVSC 1010
            WAVLKPGFLA L DPFD++P+DI+VFDVLP+S+GNG+ R++LAKE+++ NPLRH F+V+C
Sbjct: 256  WAVLKPGFLALLGDPFDTQPMDIIVFDVLPTSDGNGDGRLSLAKEIREPNPLRHSFKVAC 315

Query: 1009 GTRMIKIRTRSKAKAKDWVSAINDAGLRPPEGWCHPHRFGSFAPPRGLTEDGSQAQWFVD 830
            G R I+IR ++ +K KDWV+AINDAGLRPPEGWCHPHRFGS+APPRGLT+DGS+AQWF+D
Sbjct: 316  GNRSIRIRAKTGSKVKDWVAAINDAGLRPPEGWCHPHRFGSYAPPRGLTDDGSKAQWFID 375

Query: 829  GRAAFEAIALAIEDSKSEIFICGWWLCPDLYLRRPFHIHASSRLDSLLEAKAKQGVQIYI 650
            G AAFEAIA +IE +KSEIFICGWWLCP+LYLRRPF  +ASSRLD+LLEAKAK+GVQIYI
Sbjct: 376  GLAAFEAIAFSIERAKSEIFICGWWLCPELYLRRPFVSNASSRLDALLEAKAKEGVQIYI 435

Query: 649  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSSGVYFWSHHEKIVVVDHHICFI 470
            LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFS GVY WSHHEK+V+VD+HICFI
Sbjct: 436  LLYKEVALALKINSVYSKRKLLSIHENVRVLRYPDHFSCGVYLWSHHEKLVIVDYHICFI 495

Query: 469  GGLDLCFGRFDSIEHKVGDNPPEIWPGKDYYNPRESEPNSWEDTMKDELDRGKYPRMPWH 290
            GGLDLCFGR+D+ EHKVGD PP +WPGKDYYNPRESEPNSWEDTM+DELDR KYPRMPWH
Sbjct: 496  GGLDLCFGRYDTPEHKVGDCPPSVWPGKDYYNPRESEPNSWEDTMRDELDRKKYPRMPWH 555

Query: 289  DVHCALWGPPCRDIARHFVQRWNYAKRSKAPNEQAIPLLMPQHHMVIPHYLGETAETEVE 110
            DVHCALWGPPCRDIARHFVQRWNYAKR+KAPNEQAIPLLMPQHHMVIPHYL  + E EVE
Sbjct: 556  DVHCALWGPPCRDIARHFVQRWNYAKRNKAPNEQAIPLLMPQHHMVIPHYLWNSRELEVE 615

Query: 109  QRDGENEPMNSIQQEQETASTRSSFQDIPLLITQEA 2
            ++  ++    ++   Q++ S  SSF DIPLL+ QEA
Sbjct: 616  KKSLDDPRETTV---QDSFSRGSSFHDIPLLLPQEA 648