BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00017568
         (1353 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADZ28515.1| flavonoid-3'-hydroxylase [Camellia nitidissima]        529   e-148
gb|AFI71898.1| flavonoid 3'-hydroxylase [Paeonia lactiflora]          528   e-147
gb|ACV74415.1| putative flavonoid-3'-hydroxylase [Camellia sinen...   521   e-145
gb|AEN71546.1| flavanone 3'-hydroxylase [Paeonia suffruticosa]        518   e-144
dbj|BAJ11756.1| flavonoid 3'-hydroxylase [Calystegia pubescens]       514   e-143

>gb|ADZ28515.1| flavonoid-3'-hydroxylase [Camellia nitidissima]
          Length = 518

 Score =  529 bits (1362), Expect = e-148
 Identities = 269/359 (74%), Positives = 301/359 (83%), Gaps = 3/359 (0%)
 Frame = +1

Query: 1    TRALVSADKKA-VNLAQLLNVCTTNALGRVLLGKRVFGDGGGGSDPKADEFKSMVVELME 177
            TRAL SA + A VNL QLLNVCTTNALGRV+LG RVFGDG G  DPKADEFK MVVELM 
Sbjct: 159  TRALASAGQAAAVNLGQLLNVCTTNALGRVMLGHRVFGDGSGSGDPKADEFKEMVVELMV 218

Query: 178  LAGVFNIGDFIPSLQMFDLQGVAGKMKKLHSRFDAFLNTILEEHKGNVIYDNKANNVDFL 357
            LAGVFNIGDF+P+L+  DLQGVA KMKKLH+RFD+FLN ILEEHK N        + D L
Sbjct: 219  LAGVFNIGDFVPALEWLDLQGVASKMKKLHARFDSFLNKILEEHKINYSGSGAQKHTDLL 278

Query: 358  SMLLSSQNE--GEGTKLNDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRNPKLLSQAQK 531
            S L+S +++  GEG KL DIEIKALLL++FTAGTDTSSSTVEW IAELIR+PK+L+QA++
Sbjct: 279  STLISLKDDADGEGGKLTDIEIKALLLDLFTAGTDTSSSTVEWAIAELIRHPKILAQAKQ 338

Query: 532  ELDGVVGPDRLPTESDLLQLPFLTAIVKETFRLHPSTPLSLPRMASEDCEINGYHIPKGS 711
            ELD +VGPDR  TESDL QL FL AI+KETFRLHPSTPLSLPRMAS+ CEINGY IPKGS
Sbjct: 339  ELDSIVGPDRRVTESDLAQLTFLQAIIKETFRLHPSTPLSLPRMASDSCEINGYFIPKGS 398

Query: 712  TLLVNIWAISRDPNAWTDPLVFRPERFMPGGEKANVDLRGNDFETIPFGAGRRICAGMSL 891
            TLLVN+WAI+RDP+AW +PL FRPERF+PGGEK NVD+RGNDFE IPFGAGRRICAGMSL
Sbjct: 399  TLLVNVWAIARDPDAWAEPLEFRPERFLPGGEKPNVDVRGNDFEVIPFGAGRRICAGMSL 458

Query: 892  GIRMVQLLTATLVHAFDWSLPSGEGSEEKLNMEEGYGLTLQRVVPLMVIPRPRLAVLAY 1068
            G+RMVQLLTATLVHAF+W L  G+ S EKL M+E YGLTLQR  PLMV PRPRLA   Y
Sbjct: 459  GLRMVQLLTATLVHAFNWDLADGQ-SAEKLKMDEAYGLTLQRAAPLMVHPRPRLAPHVY 516


>gb|AFI71898.1| flavonoid 3'-hydroxylase [Paeonia lactiflora]
          Length = 507

 Score =  528 bits (1361), Expect = e-147
 Identities = 265/356 (74%), Positives = 299/356 (83%), Gaps = 2/356 (0%)
 Frame = +1

Query: 7    ALVSADKKAVNLAQLLNVCTTNALGRVLLGKRVFGDGGGGSDPKADEFKSMVVELMELAG 186
            AL SA    VNL QLLNVCTTNAL RV+LGKRVFGDG GG DPKADEFK MVVE+M LAG
Sbjct: 155  ALTSASNSLVNLGQLLNVCTTNALARVMLGKRVFGDGSGGVDPKADEFKDMVVEVMVLAG 214

Query: 187  VFNIGDFIPSLQMFDLQGVAGKMKKLHSRFDAFLNTILEEHKGNVIYDNKANNVDFLSML 366
            VFN+GDF+P+L+ FDLQGVA KMK LH+RFD+FL  ILEEHK N   D      DFLS L
Sbjct: 215  VFNLGDFVPALERFDLQGVATKMKNLHARFDSFLGNILEEHKMNS--DGVKQQNDFLSKL 272

Query: 367  LSSQNE--GEGTKLNDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRNPKLLSQAQKELD 540
            +S +++  GEG KL DIEIKALLLNMFTAGTDTSSSTVEWGIAELIR+PK+L+QAQ+E+D
Sbjct: 273  ISLKDDVDGEGGKLTDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRHPKILAQAQQEID 332

Query: 541  GVVGPDRLPTESDLLQLPFLTAIVKETFRLHPSTPLSLPRMASEDCEINGYHIPKGSTLL 720
             VVG DRL TE DL  LPFL A+VKETFRLHPSTPLSLPRMAS+ CEINGY+IPKGSTLL
Sbjct: 333  SVVGRDRLVTELDLPNLPFLQAVVKETFRLHPSTPLSLPRMASQSCEINGYYIPKGSTLL 392

Query: 721  VNIWAISRDPNAWTDPLVFRPERFMPGGEKANVDLRGNDFETIPFGAGRRICAGMSLGIR 900
            VN+WAI+RDPN W +PL FRP+RF+PGGEK N+D++GNDFE IPFGAGRRICAGMSLG+R
Sbjct: 393  VNVWAIARDPNVWAEPLEFRPDRFLPGGEKPNIDIKGNDFEVIPFGAGRRICAGMSLGLR 452

Query: 901  MVQLLTATLVHAFDWSLPSGEGSEEKLNMEEGYGLTLQRVVPLMVIPRPRLAVLAY 1068
            MVQLLTATLVHAFDW LP G+   EKL MEE YGLTLQR VPL++ P+PRL+   Y
Sbjct: 453  MVQLLTATLVHAFDWGLPEGQ-IPEKLQMEEAYGLTLQRAVPLVLYPQPRLSSHVY 507


>gb|ACV74415.1| putative flavonoid-3'-hydroxylase [Camellia sinensis]
          Length = 518

 Score =  521 bits (1342), Expect = e-145
 Identities = 267/359 (74%), Positives = 297/359 (82%), Gaps = 3/359 (0%)
 Frame = +1

Query: 1    TRALVSADKKA-VNLAQLLNVCTTNALGRVLLGKRVFGDGGGGSDPKADEFKSMVVELME 177
            TRAL SA + A VNL QLLNVCTTNALGRV+LG RVFGDG G  DPKADEFK MVVELM 
Sbjct: 159  TRALESAGQAAAVNLGQLLNVCTTNALGRVMLGHRVFGDGSGSGDPKADEFKEMVVELMV 218

Query: 178  LAGVFNIGDFIPSLQMFDLQGVAGKMKKLHSRFDAFLNTILEEHKGNVIYDNKANNVDFL 357
            LAGVFNIGDF+P+L+  D QGVA KMKKLH+RFD+FLN ILEEHK N        + D L
Sbjct: 219  LAGVFNIGDFVPALEWLDPQGVASKMKKLHARFDSFLNKILEEHKVNYSSSGAQKHTDLL 278

Query: 358  SMLLSSQNE--GEGTKLNDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRNPKLLSQAQK 531
            S L+S +++  GEG KL DIEIKALLL++FTAGTDTSSSTVEW IAELIR  K+L+QA++
Sbjct: 279  STLISLKDDADGEGGKLTDIEIKALLLDLFTAGTDTSSSTVEWAIAELIRLSKILAQAKQ 338

Query: 532  ELDGVVGPDRLPTESDLLQLPFLTAIVKETFRLHPSTPLSLPRMASEDCEINGYHIPKGS 711
            ELD +VGPDR  TESDL QL FL AI+KETFRLHPSTPLSLPRMAS+ CEINGY IPKGS
Sbjct: 339  ELDSIVGPDRRVTESDLAQLTFLQAIIKETFRLHPSTPLSLPRMASDSCEINGYFIPKGS 398

Query: 712  TLLVNIWAISRDPNAWTDPLVFRPERFMPGGEKANVDLRGNDFETIPFGAGRRICAGMSL 891
            TLLVN+WAI+RDP+AW +PL FRPERF+PGGEK N D+RGNDFE IPFGAGRRICAGMSL
Sbjct: 399  TLLVNVWAIARDPDAWAEPLEFRPERFLPGGEKPNADVRGNDFEVIPFGAGRRICAGMSL 458

Query: 892  GIRMVQLLTATLVHAFDWSLPSGEGSEEKLNMEEGYGLTLQRVVPLMVIPRPRLAVLAY 1068
            G+RMVQLLTATLVHAFDW L  G+ S EKLNM+E YGLTLQR  PLMV P PRLA   Y
Sbjct: 459  GLRMVQLLTATLVHAFDWDLADGQ-STEKLNMDEAYGLTLQRAAPLMVHPWPRLAPHVY 516


>gb|AEN71546.1| flavanone 3'-hydroxylase [Paeonia suffruticosa]
          Length = 512

 Score =  518 bits (1334), Expect = e-144
 Identities = 262/358 (73%), Positives = 299/358 (83%), Gaps = 2/358 (0%)
 Frame = +1

Query: 1    TRALVSADKKAVNLAQLLNVCTTNALGRVLLGKRVFGDGGGGSDPKADEFKSMVVELMEL 180
            T AL SA    VNL QLLNVCTTNAL RV+LGKRVFGDG GG+DPKADEFK MVVE+M L
Sbjct: 158  THALASARDSLVNLGQLLNVCTTNALARVMLGKRVFGDGSGGADPKADEFKGMVVEVMVL 217

Query: 181  AGVFNIGDFIPSLQMFDLQGVAGKMKKLHSRFDAFLNTILEEHKGNVIYDNKANNVDFLS 360
            AGVF++GDFIP+L+ FDLQGVA KMK LH+RFD+FL  ILEEHK N   D      DFLS
Sbjct: 218  AGVFDLGDFIPALERFDLQGVATKMKNLHARFDSFLGNILEEHKMN--RDGVKQQNDFLS 275

Query: 361  MLLSSQNE--GEGTKLNDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRNPKLLSQAQKE 534
             L+S +N+  GEG KL DIEIKALLLNMFTAGTDTSSSTVEW IAELIR+PK+L+QAQ+E
Sbjct: 276  KLISLKNDVDGEGGKLTDIEIKALLLNMFTAGTDTSSSTVEWAIAELIRHPKILAQAQQE 335

Query: 535  LDGVVGPDRLPTESDLLQLPFLTAIVKETFRLHPSTPLSLPRMASEDCEINGYHIPKGST 714
            +D VVG DRL  E DL  LPFL A+VKETFRLHPSTPLSLPRMAS+ CEINGY+IPKGST
Sbjct: 336  IDSVVGRDRLVIELDLPNLPFLQAVVKETFRLHPSTPLSLPRMASQSCEINGYYIPKGST 395

Query: 715  LLVNIWAISRDPNAWTDPLVFRPERFMPGGEKANVDLRGNDFETIPFGAGRRICAGMSLG 894
            LLVN+WAI+RDP+ W +PL FRP+RF+ GGEK NVD++GN+FE +PFGAGRRICAGMSLG
Sbjct: 396  LLVNVWAIARDPDVWAEPLEFRPDRFLLGGEKPNVDIKGNNFEVVPFGAGRRICAGMSLG 455

Query: 895  IRMVQLLTATLVHAFDWSLPSGEGSEEKLNMEEGYGLTLQRVVPLMVIPRPRLAVLAY 1068
            +RMVQLLTATLVHAFDW LP+G+   EKL MEE YGLTLQR VPL++ P+PRL+   Y
Sbjct: 456  LRMVQLLTATLVHAFDWGLPAGQ-IPEKLEMEEAYGLTLQRAVPLVLHPQPRLSSHVY 512


>dbj|BAJ11756.1| flavonoid 3'-hydroxylase [Calystegia pubescens]
          Length = 520

 Score =  514 bits (1325), Expect = e-143
 Identities = 262/357 (73%), Positives = 296/357 (82%), Gaps = 6/357 (1%)
 Frame = +1

Query: 1    TRALVSADKKAVNLAQLLNVCTTNALGRVLLGKRVFGD---GGGGSDPKADEFKSMVVEL 171
            TRAL SA K  V L QLLNVCTTNAL RVLLG++VF D    GG SDPKADEFKSMV E+
Sbjct: 159  TRALASAGKTPVKLGQLLNVCTTNALSRVLLGRKVFNDQTNAGGKSDPKADEFKSMVEEM 218

Query: 172  MELAGVFNIGDFIPSLQMFDLQGVAGKMKKLHSRFDAFLNTILEEHKG-NVIYDNKANNV 348
            M LAG FNIGDFIP+L+  DLQG+ GKMKKLH+RFDAFLNTILEEHK  N + +  + +V
Sbjct: 219  MVLAGTFNIGDFIPALEWLDLQGIVGKMKKLHARFDAFLNTILEEHKVINNVSNKMSQDV 278

Query: 349  DFLSMLLSSQNEGEGT--KLNDIEIKALLLNMFTAGTDTSSSTVEWGIAELIRNPKLLSQ 522
            DFLS ++S ++ G+G   KL D EIKALLLN+FTAGTDTSSSTVEW IAELIRNP +L Q
Sbjct: 279  DFLSTIISLKDNGDGEEEKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAELIRNPIILKQ 338

Query: 523  AQKELDGVVGPDRLPTESDLLQLPFLTAIVKETFRLHPSTPLSLPRMASEDCEINGYHIP 702
            AQ ELD VVGP+R+ TE DL QLPF  AIVKETFRLHPSTPLSLPRMASE+CEINGY IP
Sbjct: 339  AQSELDSVVGPNRVVTEPDLAQLPFTQAIVKETFRLHPSTPLSLPRMASENCEINGYFIP 398

Query: 703  KGSTLLVNIWAISRDPNAWTDPLVFRPERFMPGGEKANVDLRGNDFETIPFGAGRRICAG 882
            KG+TLLVN+WAI+RDPN WTDPL F P RF+PGGEK +VD++GNDFE IPFGAGRRIC+G
Sbjct: 399  KGATLLVNVWAIARDPNVWTDPLEFNPARFLPGGEKPSVDIKGNDFEVIPFGAGRRICSG 458

Query: 883  MSLGIRMVQLLTATLVHAFDWSLPSGEGSEEKLNMEEGYGLTLQRVVPLMVIPRPRL 1053
            MSLG+RMV LL ATL+H+FDW L SG+ S E LNMEE YGLTLQR VPLMV P+PRL
Sbjct: 459  MSLGLRMVHLLIATLIHSFDWDLASGQ-SIETLNMEEAYGLTLQRAVPLMVHPKPRL 514