BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00016425
         (1461 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI27043.3| unnamed protein product [Vitis vinifera]              634   e-179
ref|XP_002278978.1| PREDICTED: aluminum-activated malate transpo...   634   e-179
ref|XP_002312386.1| predicted protein [Populus trichocarpa] gi|2...   588   e-165
ref|XP_002529137.1| conserved hypothetical protein [Ricinus comm...   587   e-165
ref|XP_004170574.1| PREDICTED: LOW QUALITY PROTEIN: aluminum-act...   582   e-163

>emb|CBI27043.3| unnamed protein product [Vitis vinifera]
          Length = 1070

 Score =  634 bits (1636), Expect = e-179
 Identities = 324/489 (66%), Positives = 380/489 (77%), Gaps = 3/489 (0%)
 Frame = -1

Query: 1458 YQMGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGATL 1279
            ++M + DPRK  FA KMGL+LA VS+ IF KEPL  +SQYSIWAILTVVVVFEFS+GATL
Sbjct: 555  FEMARSDPRKVYFAAKMGLSLAIVSLFIFLKEPLKDVSQYSIWAILTVVVVFEFSVGATL 614

Query: 1278 SKGFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYEY 1099
            SKGFNRALGTFSAGGL+LGIAELS+L G  +EV+++ISIFIAGFCASY KLYP  K YEY
Sbjct: 615  SKGFNRALGTFSAGGLALGIAELSMLTGALEEVIIIISIFIAGFCASYCKLYPEMKPYEY 674

Query: 1098 GFRVFLLTYCIVLVSGA--SHFTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKLV 925
            GFRVFLLT+CIVLVSG+  S F  TA+ RLL IGVGA +CL++N C+ PIWAGEDLHKLV
Sbjct: 675  GFRVFLLTFCIVLVSGSTSSKFLQTALYRLLFIGVGAGICLVVNTCICPIWAGEDLHKLV 734

Query: 924  VKNIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLLG 745
            VKN +GVATSLEGCVN YLQ VEYERIPSKIL YQASDD VY+GYR+ V+STSQE+SLL 
Sbjct: 735  VKNFQGVATSLEGCVNEYLQCVEYERIPSKILTYQASDDPVYNGYRSVVQSTSQEDSLLD 794

Query: 744  FAEWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNGI 565
            FA WEPPHG Y+MF+YPW  YVKVSGALRHCAFMVMAMHGCILSEIQ+  E RQVF + +
Sbjct: 795  FAIWEPPHGHYRMFHYPWKSYVKVSGALRHCAFMVMAMHGCILSEIQAPPEKRQVFSSEL 854

Query: 564  QKVGVEGANVLRLLGEKVEKMEKLSSEDLLETVHEAAEVLQMQIDQKSYLLVKAESWERA 385
            Q+VGVEGA VLR LG KVEKMEKL  +DLL  VHEAAE LQM+ID+ S+LLV   SWE  
Sbjct: 855  QRVGVEGAKVLRELGRKVEKMEKLGQQDLLIEVHEAAEELQMKIDKNSFLLVNFASWEAG 914

Query: 384  KRPKKFEDPEQLQELTENEPKPMMISSLSNLHLKSTQAMRPFDPNNPNMSINVSAQ-WES 208
            + PK++ED E + ++ + E K  +I+SLS   L    A R ++   PNMS++     W S
Sbjct: 915  RLPKEYEDAENILQVKDTELKTPVITSLSETVLDLGSAPRSWNAQTPNMSMDPPMPGWVS 974

Query: 207  TEDMLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLVDAF 28
            +E M ++Q  WPS LS   D+VLNE+E +TYE             LIEFVARLQ LVD+F
Sbjct: 975  SESMFKKQVSWPSGLSFNADLVLNEQESKTYESASSLSLATFTSLLIEFVARLQYLVDSF 1034

Query: 27   TDLSEKAKF 1
             +LSE AKF
Sbjct: 1035 EELSELAKF 1043



 Score =  561 bits (1445), Expect = e-157
 Identities = 290/486 (59%), Positives = 354/486 (72%), Gaps = 2/486 (0%)
 Frame = -1

Query: 1452 MGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGATLSK 1273
            MG+ DPRK IFA+KMGLAL+ VS+LIF+KEP   + QYSIWAILTV+V+FEFSIGAT  K
Sbjct: 1    MGRSDPRKIIFAMKMGLALSLVSLLIFWKEPAD-VGQYSIWAILTVIVMFEFSIGATFIK 59

Query: 1272 GFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYEYGF 1093
            GFNR LGT  AG L+ G AELS+LAG  +EVV+VISIFI GF  SYLKLYP    YEYGF
Sbjct: 60   GFNRGLGTLCAGILAFGFAELSVLAGPCEEVVIVISIFITGFFTSYLKLYPTMAPYEYGF 119

Query: 1092 RVFLLTYCIVLVSG--ASHFTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKLVVK 919
            RVF++TYCI++++G     +    V RL+LI VG  VC I+N+C YPIWAGEDLH LVVK
Sbjct: 120  RVFIMTYCILMMAGNRTREYNQAVVIRLVLIAVGGGVCFIVNICFYPIWAGEDLHSLVVK 179

Query: 918  NIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLLGFA 739
            N KGVATSLEGCVN YL+ V+YER+P KI  +QASDD + +GYR+ VESTS+E +LLGFA
Sbjct: 180  NFKGVATSLEGCVNGYLKCVQYERVPQKIHTHQASDDPLSNGYRSVVESTSREATLLGFA 239

Query: 738  EWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNGIQK 559
             WEPPHGRY+MFNYPW  YVK+SGALRHCAFMVMA+HGCILSEIQ+ +E R VF++ +Q+
Sbjct: 240  IWEPPHGRYRMFNYPWKNYVKLSGALRHCAFMVMALHGCILSEIQAPAERRLVFQSELQR 299

Query: 558  VGVEGANVLRLLGEKVEKMEKLSSEDLLETVHEAAEVLQMQIDQKSYLLVKAESWERAKR 379
            VG EGA VLR L  KVEKMEKLS  D+L+ VHEAAE LQ +IDQ+SYLLV +ESW    R
Sbjct: 300  VGTEGAKVLRELANKVEKMEKLSPGDILKEVHEAAEQLQKKIDQRSYLLVNSESW-LIGR 358

Query: 378  PKKFEDPEQLQELTENEPKPMMISSLSNLHLKSTQAMRPFDPNNPNMSINVSAQWESTED 199
             ++ EDP  L+++ +NE   +   SLS   L+                I     W  + D
Sbjct: 359  TREVEDPVNLEDVKDNENVKLGSKSLSETVLE----------------IRSFLAWPPSGD 402

Query: 198  MLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLVDAFTDL 19
            + R+Q+ WPSR S + D V+ E E+RTYE             LIEFVARLQN+VD+F +L
Sbjct: 403  VFRKQSPWPSRPSFIADAVIREDEIRTYESASALSLATFVSLLIEFVARLQNVVDSFQEL 462

Query: 18   SEKAKF 1
            SEKA+F
Sbjct: 463  SEKAEF 468


>ref|XP_002278978.1| PREDICTED: aluminum-activated malate transporter 4 [Vitis vinifera]
          Length = 583

 Score =  634 bits (1636), Expect = e-179
 Identities = 324/489 (66%), Positives = 380/489 (77%), Gaps = 3/489 (0%)
 Frame = -1

Query: 1458 YQMGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGATL 1279
            ++M + DPRK  FA KMGL+LA VS+ IF KEPL  +SQYSIWAILTVVVVFEFS+GATL
Sbjct: 68   FEMARSDPRKVYFAAKMGLSLAIVSLFIFLKEPLKDVSQYSIWAILTVVVVFEFSVGATL 127

Query: 1278 SKGFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYEY 1099
            SKGFNRALGTFSAGGL+LGIAELS+L G  +EV+++ISIFIAGFCASY KLYP  K YEY
Sbjct: 128  SKGFNRALGTFSAGGLALGIAELSMLTGALEEVIIIISIFIAGFCASYCKLYPEMKPYEY 187

Query: 1098 GFRVFLLTYCIVLVSGA--SHFTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKLV 925
            GFRVFLLT+CIVLVSG+  S F  TA+ RLL IGVGA +CL++N C+ PIWAGEDLHKLV
Sbjct: 188  GFRVFLLTFCIVLVSGSTSSKFLQTALYRLLFIGVGAGICLVVNTCICPIWAGEDLHKLV 247

Query: 924  VKNIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLLG 745
            VKN +GVATSLEGCVN YLQ VEYERIPSKIL YQASDD VY+GYR+ V+STSQE+SLL 
Sbjct: 248  VKNFQGVATSLEGCVNEYLQCVEYERIPSKILTYQASDDPVYNGYRSVVQSTSQEDSLLD 307

Query: 744  FAEWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNGI 565
            FA WEPPHG Y+MF+YPW  YVKVSGALRHCAFMVMAMHGCILSEIQ+  E RQVF + +
Sbjct: 308  FAIWEPPHGHYRMFHYPWKSYVKVSGALRHCAFMVMAMHGCILSEIQAPPEKRQVFSSEL 367

Query: 564  QKVGVEGANVLRLLGEKVEKMEKLSSEDLLETVHEAAEVLQMQIDQKSYLLVKAESWERA 385
            Q+VGVEGA VLR LG KVEKMEKL  +DLL  VHEAAE LQM+ID+ S+LLV   SWE  
Sbjct: 368  QRVGVEGAKVLRELGRKVEKMEKLGQQDLLIEVHEAAEELQMKIDKNSFLLVNFASWEAG 427

Query: 384  KRPKKFEDPEQLQELTENEPKPMMISSLSNLHLKSTQAMRPFDPNNPNMSINVSAQ-WES 208
            + PK++ED E + ++ + E K  +I+SLS   L    A R ++   PNMS++     W S
Sbjct: 428  RLPKEYEDAENILQVKDTELKTPVITSLSETVLDLGSAPRSWNAQTPNMSMDPPMPGWVS 487

Query: 207  TEDMLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLVDAF 28
            +E M ++Q  WPS LS   D+VLNE+E +TYE             LIEFVARLQ LVD+F
Sbjct: 488  SESMFKKQVSWPSGLSFNADLVLNEQESKTYESASSLSLATFTSLLIEFVARLQYLVDSF 547

Query: 27   TDLSEKAKF 1
             +LSE AKF
Sbjct: 548  EELSELAKF 556


>ref|XP_002312386.1| predicted protein [Populus trichocarpa] gi|222852206|gb|EEE89753.1|
            predicted protein [Populus trichocarpa]
          Length = 544

 Score =  588 bits (1516), Expect = e-165
 Identities = 315/489 (64%), Positives = 371/489 (75%), Gaps = 3/489 (0%)
 Frame = -1

Query: 1458 YQMGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGATL 1279
            Y+MG+ DPRK++FAVKMGL+LA VS++IF KEPL  +SQYSIWAILTVVVVFEFS+GATL
Sbjct: 69   YKMGQADPRKYLFAVKMGLSLALVSLVIFLKEPLKDVSQYSIWAILTVVVVFEFSVGATL 128

Query: 1278 SKGFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYEY 1099
            +KGFNRALGTFSAG L++GIAELSL  G   EV++V+SIFIAGF ASY+KLYP  K YEY
Sbjct: 129  NKGFNRALGTFSAGALAIGIAELSLHVGALGEVLLVVSIFIAGFFASYIKLYPTMKPYEY 188

Query: 1098 GFRVFLLTYCIVLVSGASH-FTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKLVV 922
            GFRVFLLTYCIV VSG+S  F +TAV RLLLI VGAA+CL +N+C++PIWAGEDLHKLVV
Sbjct: 189  GFRVFLLTYCIVTVSGSSSSFFHTAVYRLLLIAVGAAICLAVNICIFPIWAGEDLHKLVV 248

Query: 921  KNIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLLGF 742
            KN  GVA SLEGCVN YLQ VEYERIPSKIL Y+ASDD +YSGYR+AV+STSQEESLL F
Sbjct: 249  KNFNGVANSLEGCVNGYLQCVEYERIPSKILTYEASDDPLYSGYRSAVQSTSQEESLLSF 308

Query: 741  AEWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNGIQ 562
            A WEPPHG Y+ FNYPW  YVK+SG+LRHCAFMVMAMHG ILSEIQ+  E RQVF + +Q
Sbjct: 309  AIWEPPHGPYRSFNYPWKNYVKLSGSLRHCAFMVMAMHGSILSEIQAPPEKRQVFSSELQ 368

Query: 561  KVGVEGANVLRLLGEKVEKMEKLS-SEDLLETVHEAAEVLQMQIDQKSYLLVKAESWERA 385
            +VG EGA VLR LG+KVEKMEKL    D+L  VHEAAE LQM+IDQ SYLLV +ESW   
Sbjct: 369  RVGNEGAKVLRELGKKVEKMEKLGPGVDVLLEVHEAAEELQMKIDQNSYLLVNSESWAAG 428

Query: 384  KRPKKFEDPEQLQELTENEPKPMMISSLSNLHLKSTQAMRPFDPNNPNMSINVS-AQWES 208
            +  K+FEDP   Q L E+E K  +IS LS            +D  N N+S + S  + ++
Sbjct: 429  RPAKEFEDP---QNLLEDESK--LISYLS----------ETWDVKNQNISTSPSMPELKA 473

Query: 207  TEDMLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLVDAF 28
            ++ +  Q   WP RLS  G  ++ E+E + YE             LIEFVARLQNL D F
Sbjct: 474  SDSVFNQPVSWP-RLSFTGGSMIVEQESKVYESASSLSLATFASLLIEFVARLQNLADEF 532

Query: 27   TDLSEKAKF 1
             +LSEKA F
Sbjct: 533  QELSEKANF 541


>ref|XP_002529137.1| conserved hypothetical protein [Ricinus communis]
            gi|223531416|gb|EEF33250.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 574

 Score =  587 bits (1514), Expect = e-165
 Identities = 317/492 (64%), Positives = 373/492 (75%), Gaps = 6/492 (1%)
 Frame = -1

Query: 1458 YQMGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGATL 1279
            Y MG+ DPRK  FAVKMGL+LA VS++IF KEPL  ++QYSIWAILTVVVVFEFS+GATL
Sbjct: 70   YNMGRTDPRKVFFAVKMGLSLALVSLVIFLKEPLKNVNQYSIWAILTVVVVFEFSVGATL 129

Query: 1278 SKGFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYEY 1099
            +KGFNRALGT SAGGL+LGIAELSL AG + EV VVISIFIAGFCASY+KL+P+ K YEY
Sbjct: 130  NKGFNRALGTLSAGGLALGIAELSLFAGNFVEVFVVISIFIAGFCASYIKLHPSMKSYEY 189

Query: 1098 GFRVFLLTYCIVLVSGASH-FTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKLVV 922
            GFRVFLLTYCIV+VSG+S  F  TA  RLLLI VGA + L+IN+CV+PIWAGEDLHKLVV
Sbjct: 190  GFRVFLLTYCIVMVSGSSSTFVETAFYRLLLIAVGAGIGLVINICVFPIWAGEDLHKLVV 249

Query: 921  KNIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLLGF 742
            KN KGVA SLEGCVN YLQ VEYERIPSKIL YQASDD +YSGYR+AV+S+SQEESLL F
Sbjct: 250  KNFKGVAASLEGCVNGYLQCVEYERIPSKILTYQASDDPLYSGYRSAVQSSSQEESLLDF 309

Query: 741  AEWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNGIQ 562
            A WEPPHG YK FNYPW  Y+K+SGALRHCAFMVMAMHGCILSEIQ+ +E RQVF + +Q
Sbjct: 310  AIWEPPHGPYKSFNYPWKNYLKLSGALRHCAFMVMAMHGCILSEIQAPAEKRQVFCSELQ 369

Query: 561  KVGVEGANVLRLLGEKVEKMEKL-SSEDLLETVHEAAEVLQMQIDQKSYLLVKAESWERA 385
            KVG EGA +LR LG +VEKMEKL   +D+L  V EAAE LQ++IDQKSY+LV ++SW   
Sbjct: 370  KVGNEGAKILRELGNRVEKMEKLIPGDDILLEVQEAAEGLQLKIDQKSYILVNSDSWAAE 429

Query: 384  K-RPKKFEDPEQLQELTENEPKPMMISSLSNLHLKSTQAMRPFDPNNPNMSINVS---AQ 217
            + +PK+ EDP  + EL +NE +  +I+ +S             D   PN S+N S   A+
Sbjct: 430  RVQPKELEDPGSINELEDNEGE--VINCIS----------ETLDDQYPNTSMNPSSTQAE 477

Query: 216  WESTEDMLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLV 37
               +E+ML++   WP   S +    L E+E R YE             LIEFVARLQNLV
Sbjct: 478  RMQSENMLKRS--WP---SFIASSRLIEQESRVYESASSLSLATFASLLIEFVARLQNLV 532

Query: 36   DAFTDLSEKAKF 1
            DAF +LSE A F
Sbjct: 533  DAFQELSEMANF 544


>ref|XP_004170574.1| PREDICTED: LOW QUALITY PROTEIN: aluminum-activated malate transporter
            9-like [Cucumis sativus]
          Length = 579

 Score =  582 bits (1499), Expect = e-163
 Identities = 293/491 (59%), Positives = 370/491 (75%), Gaps = 4/491 (0%)
 Frame = -1

Query: 1461 AYQMGKKDPRKFIFAVKMGLALAFVSILIFFKEPLSYISQYSIWAILTVVVVFEFSIGAT 1282
            A+QMG  DPRK +F+ KMGLAL  +S+LIFFK+P+  +S+YS+WAILTVVVVFEFSIGAT
Sbjct: 65   AWQMGVSDPRKIVFSAKMGLALTLISLLIFFKQPVEELSRYSVWAILTVVVVFEFSIGAT 124

Query: 1281 LSKGFNRALGTFSAGGLSLGIAELSLLAGKYQEVVVVISIFIAGFCASYLKLYPAFKQYE 1102
            LSKG NR +GT SAGGL+LG+AELS+LAG+++EVVVV SIFI GF A+Y KLYP  K YE
Sbjct: 125  LSKGLNRGIGTLSAGGLALGMAELSVLAGQWEEVVVVTSIFIMGFFATYAKLYPTMKPYE 184

Query: 1101 YGFRVFLLTYCIVLVSG--ASHFTYTAVSRLLLIGVGAAVCLIINVCVYPIWAGEDLHKL 928
            YGFRVFLLTYC ++VSG     F +TAV+R LLI +GA VCL++N+C+YPIWAGEDLH L
Sbjct: 185  YGFRVFLLTYCFIMVSGYRTREFIHTAVTRFLLIALGAGVCLVVNICIYPIWAGEDLHNL 244

Query: 927  VVKNIKGVATSLEGCVNSYLQNVEYERIPSKILIYQASDDVVYSGYRAAVESTSQEESLL 748
            VVKN  GVA SLEGCV+SYL  VEYERIPSKIL YQASDD +Y GYR+A+ES SQEE+L+
Sbjct: 245  VVKNFGGVAASLEGCVDSYLNCVEYERIPSKILTYQASDDPLYKGYRSAMESLSQEETLM 304

Query: 747  GFAEWEPPHGRYKMFNYPWSEYVKVSGALRHCAFMVMAMHGCILSEIQSSSELRQVFKNG 568
            GFA WEPPHGRY+M  YPW  YVKV+GALRHCAF +MA+HGCILSEIQ+S+E RQVF + 
Sbjct: 305  GFAIWEPPHGRYRMLKYPWKNYVKVAGALRHCAFAIMALHGCILSEIQASAERRQVFGSE 364

Query: 567  IQKVGVEGANVLRLLGEKVEKMEKLSSEDLLETVHEAAEVLQMQIDQKSYLLVKAESWER 388
            +++VG EGA VLR LG K++KMEKL S  +L  VH+AAE LQ +ID KSYLLV +ESWE 
Sbjct: 365  LRRVGYEGAKVLRELGNKLKKMEKLDSASILSEVHDAAEELQKKIDAKSYLLVNSESWEI 424

Query: 387  AKRPKKFEDPEQLQELTENEPKPMMISSLSN--LHLKSTQAMRPFDPNNPNMSINVSAQW 214
              RP+    P++L  L + E +     SLS   L L++   ++ +D +  +   ++++  
Sbjct: 425  GNRPEDVGQPQELLNLDDEEIRFREYRSLSEAVLDLRTLPILKSWDDSASSDINSITSTP 484

Query: 213  ESTEDMLRQQTMWPSRLSILGDVVLNEREVRTYEXXXXXXXXXXXXXLIEFVARLQNLVD 34
                 M ++   WP+++S+  + V++E E +TYE             LIEFVARLQNLVD
Sbjct: 485  XPPSKMFKKLGSWPAQVSVKQNGVIHEEESKTYENASALSLATFTSLLIEFVARLQNLVD 544

Query: 33   AFTDLSEKAKF 1
            +F +LSEKAKF
Sbjct: 545  SFDELSEKAKF 555