BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00016114
         (774 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACQ91100.1| GSH1 [Solanum lycopersicum]                            378   e-103
ref|NP_001234010.1| glutamate--cysteine ligase, chloroplastic [S...   376   e-102
gb|AFP93564.1| GCS [Cestrum nocturnum]                                375   e-102
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   375   e-102
ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   368   e-100

>gb|ACQ91100.1| GSH1 [Solanum lycopersicum]
          Length = 523

 Score =  378 bits (970), Expect = e-103
 Identities = 186/248 (75%), Positives = 215/248 (86%), Gaps = 1/248 (0%)
 Frame = +2

Query: 32  MSLMSQTGSSYYISNELGQCKGGY-AMPYTAGASKVKEVRFGLCFSSVESSKATQSLYAD 208
           M+LMSQ GSS+ I +E  +C  G+ ++       +++E+ FG+  SS  +S+  Q  Y +
Sbjct: 1   MALMSQAGSSHCIYSEKVRCISGHRSIINNMDMFRMREICFGVDISSRNASRRVQGNYLN 60

Query: 209 GARIGSRRGNKSIVAASPPTEDAVVAADPLTKQDLIDYLASGCKSKEKWRIGTEHEKFGF 388
              +GSRRG+ +IVAASPPTEDAVVAA+PLTK+DL+ YLASGCKSKEKWRIGTEHEKFGF
Sbjct: 61  HIGVGSRRGDLTIVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGF 120

Query: 389 EIGTLQPMKYDQISELLNGISERFEWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPL 568
           E GTL+PMKYDQI++LLNGI+ERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPL
Sbjct: 121 EFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPL 180

Query: 569 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMRNYMPKV 748
           ETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKWG+KDIP+MPKGRYEIMRNYMPKV
Sbjct: 181 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIMRNYMPKV 240

Query: 749 GTLGLDMM 772
           G+LGLDMM
Sbjct: 241 GSLGLDMM 248


>ref|NP_001234010.1| glutamate--cysteine ligase, chloroplastic [Solanum lycopersicum]
           gi|3913791|sp|O22493.1|GSH1_SOLLC RecName:
           Full=Glutamate--cysteine ligase, chloroplastic; AltName:
           Full=Gamma-ECS; Short=GCS; AltName:
           Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
           gi|2407615|gb|AAB71230.1| gamma-glutamylcysteine
           synthetase [Solanum lycopersicum]
          Length = 523

 Score =  376 bits (966), Expect = e-102
 Identities = 185/248 (74%), Positives = 215/248 (86%), Gaps = 1/248 (0%)
 Frame = +2

Query: 32  MSLMSQTGSSYYISNELGQCKGGY-AMPYTAGASKVKEVRFGLCFSSVESSKATQSLYAD 208
           M+LMSQ GSS+ I +E  +C  G+ ++       +++E+ FG+  SS  +S+  Q  Y +
Sbjct: 1   MALMSQAGSSHCIYSEKVRCISGHRSIINNMDMFRMREICFGVDISSRNASRRVQGNYLN 60

Query: 209 GARIGSRRGNKSIVAASPPTEDAVVAADPLTKQDLIDYLASGCKSKEKWRIGTEHEKFGF 388
              +GSRRG+ +IVAASPPTEDAVVAA+PLTK+DL+ YLASGCKSKEKWRIGTEHEKFGF
Sbjct: 61  HIGVGSRRGDLTIVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGF 120

Query: 389 EIGTLQPMKYDQISELLNGISERFEWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPL 568
           E GTL+PMKYDQI++LLNGI+ERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPL
Sbjct: 121 EFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPL 180

Query: 569 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMRNYMPKV 748
           ETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKWG+KDIP+MPKGRYEI+RNYMPKV
Sbjct: 181 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIIRNYMPKV 240

Query: 749 GTLGLDMM 772
           G+LGLDMM
Sbjct: 241 GSLGLDMM 248


>gb|AFP93564.1| GCS [Cestrum nocturnum]
          Length = 518

 Score =  375 bits (964), Expect = e-102
 Identities = 190/247 (76%), Positives = 208/247 (84%)
 Frame = +2

Query: 32  MSLMSQTGSSYYISNELGQCKGGYAMPYTAGASKVKEVRFGLCFSSVESSKATQSLYADG 211
           M+LMSQ GSS+ I  E  +CKGG+ M       K+KE+ FG+  S   SS A Q +    
Sbjct: 1   MALMSQAGSSHCIHPEKIRCKGGHNMEIF----KLKEICFGVHISPRNSSNAKQGICLRR 56

Query: 212 ARIGSRRGNKSIVAASPPTEDAVVAADPLTKQDLIDYLASGCKSKEKWRIGTEHEKFGFE 391
                RRG  SIVAASPPT+DAVVAA+PLTK+DL+ YLASGCKSKEKWRIGTEHEKFGFE
Sbjct: 57  VGAERRRGRLSIVAASPPTDDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGFE 116

Query: 392 IGTLQPMKYDQISELLNGISERFEWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLE 571
            GTL+PM Y+QI+ELL G+SERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPLE
Sbjct: 117 FGTLRPMNYEQIAELLYGVSERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLE 176

Query: 572 TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMRNYMPKVG 751
           TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWG+KDIPVMPKGRYEIMRNYMPKVG
Sbjct: 177 TLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMPKVG 236

Query: 752 TLGLDMM 772
           TLGLDMM
Sbjct: 237 TLGLDMM 243


>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
           Full=Gamma-ECS; Short=GCS; AltName:
           Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
           gi|111380512|gb|ABD98695.2| chloroplast
           gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  375 bits (962), Expect = e-102
 Identities = 189/248 (76%), Positives = 212/248 (85%), Gaps = 1/248 (0%)
 Frame = +2

Query: 32  MSLMSQTGSSYYISNELGQCKGGYA-MPYTAGASKVKEVRFGLCFSSVESSKATQSLYAD 208
           M+LMSQ GSS+ I +E  +C  G++ +       K+K++ FG   SS  SSK  Q +Y D
Sbjct: 1   MALMSQAGSSHCIYSEKMKCISGHSSITSNMEMLKMKDICFGN-ISSRNSSKPMQGIYLD 59

Query: 209 GARIGSRRGNKSIVAASPPTEDAVVAADPLTKQDLIDYLASGCKSKEKWRIGTEHEKFGF 388
              +  RRG  +IVAASPPTEDAVVAA+PLTK+DL+ YLASGCKSKEKWRIGTEHEKFGF
Sbjct: 60  RVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGF 119

Query: 389 EIGTLQPMKYDQISELLNGISERFEWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPL 568
           E GTL+PMKY+QI+ELLNGI+ERF+WEKVMEGD IIGLKQGKQSISLEPGGQFELSGAPL
Sbjct: 120 EFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPL 179

Query: 569 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMRNYMPKV 748
           ETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKWG+KDIPVMPKGRYEIMRNYMPKV
Sbjct: 180 ETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMPKV 239

Query: 749 GTLGLDMM 772
           G+LGLDMM
Sbjct: 240 GSLGLDMM 247


>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative
           [Ricinus communis] gi|223549699|gb|EEF51187.1|
           Glutamate--cysteine ligase, chloroplast precursor,
           putative [Ricinus communis]
          Length = 526

 Score =  368 bits (945), Expect = e-100
 Identities = 181/251 (72%), Positives = 208/251 (82%), Gaps = 4/251 (1%)
 Frame = +2

Query: 32  MSLMSQTGSSYYISNELGQCKGGY----AMPYTAGASKVKEVRFGLCFSSVESSKATQSL 199
           M ++SQ G S Y+  ++ +CK G      +  +   SK+KE        S  S+K  Q  
Sbjct: 1   MPVISQAGPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLP 60

Query: 200 YADGARIGSRRGNKSIVAASPPTEDAVVAADPLTKQDLIDYLASGCKSKEKWRIGTEHEK 379
            ++ + + SRRGN+ IVAASPP EDAV+A +PLTK+DL+ YLASGCK KEKWRIGTEHEK
Sbjct: 61  SSENSWVRSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEK 120

Query: 380 FGFEIGTLQPMKYDQISELLNGISERFEWEKVMEGDYIIGLKQGKQSISLEPGGQFELSG 559
           FGFE+GTL+PMKY+QI+ELLNGI+ERF+WEKVMEGDYIIGLKQGKQSISLEPGGQFELSG
Sbjct: 121 FGFELGTLRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSG 180

Query: 560 APLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMRNYM 739
           APLETLHQTCAEVNSHLYQVKAV EEMGIGFLGIGFQPKWG+KDIP+MPKGRYEIMRNYM
Sbjct: 181 APLETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYM 240

Query: 740 PKVGTLGLDMM 772
           PKVGTLGLDMM
Sbjct: 241 PKVGTLGLDMM 251