BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00014574
         (1645 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              694   0.0  
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   694   0.0  
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   685   0.0  
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   682   0.0  
ref|XP_003539065.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   664   0.0  

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  694 bits (1790), Expect = 0.0
 Identities = 340/421 (80%), Positives = 370/421 (87%), Gaps = 1/421 (0%)
 Frame = -2

Query: 1392 YLCCSMADKRPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1213
            YL CS+ + R LK+AVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW
Sbjct: 78   YLSCSLPNNR-LKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 136

Query: 1212 ETDLNYARAVCDQVDVALEVVHLTDEYWKNVVSYIIAEYRSGRTPNPDVLCNTRIKFGAF 1033
            E DL YA+AVCDQVDV LEVVHLTDEYWKNVVSYII EYR GRTPNPDVLCNTRIKFGAF
Sbjct: 137  EDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 196

Query: 1032 MDAISAMEFDYVASGHYAKVMHSSLLDSDEPSILQLSKDMVKDQTYFLSHLSPEQLKRLI 853
            MDAIS+ EFDYVASGHYA V+HS     D+PS+L+LSKDMVKDQTYFLSHLS  QLKRLI
Sbjct: 197  MDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQLKRLI 256

Query: 852  FPLGSMPKDEVRGLAKALNLPNQDRKDSQGICFLGKIKFSEFVARHIGEEEGPILEAETG 673
            FPLG +PKDEVR LA  L+LPN+DRKDSQGICFLGKIKFSEFVARHIGE+EG ILEAETG
Sbjct: 257  FPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETG 316

Query: 672  DLLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRLFRVGSL 493
            DLLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S+DKRRRLFRVGSL
Sbjct: 317  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSL 376

Query: 492  KWISGFSPAQVNEIQCKVRHGPGFYNCTLT-XXXXXXXXXXXXVQLAEDDQGLAAGQFAA 316
            KWIS   P Q+N++QCKVRHGPGFYNC+ T             VQL+EDDQGLAAGQF A
Sbjct: 377  KWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTA 436

Query: 315  FYKSDTCIGSGVILESWDDQGFPLCARALEMAIMEDKSKLGKPVKIKNKPESISEESDHI 136
            FY+  +CIGSGVILESWDDQGFP+CA+ALE+A MEDKSKLGKPVKIK +PE+  + SD I
Sbjct: 437  FYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQI 496

Query: 135  N 133
            +
Sbjct: 497  D 497


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  694 bits (1790), Expect = 0.0
 Identities = 340/421 (80%), Positives = 370/421 (87%), Gaps = 1/421 (0%)
 Frame = -2

Query: 1392 YLCCSMADKRPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1213
            YL CS+ + R LK+AVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW
Sbjct: 74   YLSCSLPNNR-LKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 132

Query: 1212 ETDLNYARAVCDQVDVALEVVHLTDEYWKNVVSYIIAEYRSGRTPNPDVLCNTRIKFGAF 1033
            E DL YA+AVCDQVDV LEVVHLTDEYWKNVVSYII EYR GRTPNPDVLCNTRIKFGAF
Sbjct: 133  EDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 192

Query: 1032 MDAISAMEFDYVASGHYAKVMHSSLLDSDEPSILQLSKDMVKDQTYFLSHLSPEQLKRLI 853
            MDAIS+ EFDYVASGHYA V+HS     D+PS+L+LSKDMVKDQTYFLSHLS  QLKRLI
Sbjct: 193  MDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQLKRLI 252

Query: 852  FPLGSMPKDEVRGLAKALNLPNQDRKDSQGICFLGKIKFSEFVARHIGEEEGPILEAETG 673
            FPLG +PKDEVR LA  L+LPN+DRKDSQGICFLGKIKFSEFVARHIGE+EG ILEAETG
Sbjct: 253  FPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETG 312

Query: 672  DLLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRLFRVGSL 493
            DLLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S+DKRRRLFRVGSL
Sbjct: 313  DLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSL 372

Query: 492  KWISGFSPAQVNEIQCKVRHGPGFYNCTLT-XXXXXXXXXXXXVQLAEDDQGLAAGQFAA 316
            KWIS   P Q+N++QCKVRHGPGFYNC+ T             VQL+EDDQGLAAGQF A
Sbjct: 373  KWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTA 432

Query: 315  FYKSDTCIGSGVILESWDDQGFPLCARALEMAIMEDKSKLGKPVKIKNKPESISEESDHI 136
            FY+  +CIGSGVILESWDDQGFP+CA+ALE+A MEDKSKLGKPVKIK +PE+  + SD I
Sbjct: 433  FYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQI 492

Query: 135  N 133
            +
Sbjct: 493  D 493


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  685 bits (1767), Expect = 0.0
 Identities = 334/433 (77%), Positives = 367/433 (84%), Gaps = 1/433 (0%)
 Frame = -2

Query: 1404 IEPKYLCCSMADKRPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 1225
            IEP YL C + DK  L++A+L+SGGVDSSVALRLLHAAGH CTAFYLKIWFQEDFENFWS
Sbjct: 56   IEPNYLSCCLPDKNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFWS 115

Query: 1224 ECPWETDLNYARAVCDQVDVALEVVHLTDEYWKNVVSYIIAEYRSGRTPNPDVLCNTRIK 1045
            ECPWE DL YA+AVCDQVD+ LEVVHLTDEYWKNVVSYII EYR GRTPNPDVLCNTRIK
Sbjct: 116  ECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 175

Query: 1044 FGAFMDAISAMEFDYVASGHYAKVMHSSLLDSDEPSILQLSKDMVKDQTYFLSHLSPEQL 865
            FGAFMDAI+ MEFDYVASGHYAKV+H S  + ++PS+L+LSKDMVKDQTYFLSHLS  QL
Sbjct: 176  FGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQL 235

Query: 864  KRLIFPLGSMPKDEVRGLAKALNLPNQDRKDSQGICFLGKIKFSEFVARHIGEEEGPILE 685
            +RLIFPLG + KDEVR LA   NLPN+DRKDSQGICFLGKIKFSEFVARHIGE EG +LE
Sbjct: 236  RRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLE 295

Query: 684  AETGDLLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRLFR 505
            AETGD LGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S DKRRRL R
Sbjct: 296  AETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLR 355

Query: 504  VGSLKWISGFSPAQVNEIQCKVRHGPGFYNCTLT-XXXXXXXXXXXXVQLAEDDQGLAAG 328
            VGSL+W +G  P + +E+QCKVRHGPGFYNC++              VQL EDDQGLAAG
Sbjct: 356  VGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAG 415

Query: 327  QFAAFYKSDTCIGSGVILESWDDQGFPLCARALEMAIMEDKSKLGKPVKIKNKPESISEE 148
            QFAAFYK  TCIGSGVILESW+DQGFP+C RALE+A +EDKS+LGKPVKIK KPE    E
Sbjct: 416  QFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPEIPVRE 475

Query: 147  SDHINQNKDNVKV 109
             D     KD ++V
Sbjct: 476  FD----GKDGIEV 484


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  682 bits (1761), Expect = 0.0
 Identities = 333/417 (79%), Positives = 361/417 (86%), Gaps = 1/417 (0%)
 Frame = -2

Query: 1392 YLCCSMADKRPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 1213
            +L CSM    PLKVAVL+SGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW
Sbjct: 72   FLSCSMPTY-PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPW 130

Query: 1212 ETDLNYARAVCDQVDVALEVVHLTDEYWKNVVSYIIAEYRSGRTPNPDVLCNTRIKFGAF 1033
            + DL YA+AVCDQVDV LEVVHLT+EYW+NVVSYII EYR GRTPNPDVLCNTRIKFGAF
Sbjct: 131  DDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF 190

Query: 1032 MDAISAMEFDYVASGHYAKVMHSSLLDSDEPSILQLSKDMVKDQTYFLSHLSPEQLKRLI 853
            MDAIS MEFDYVASGHYA V+H S    D+PS+L+LSKDMVKDQTYFLSHLS  QLKRL+
Sbjct: 191  MDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLL 250

Query: 852  FPLGSMPKDEVRGLAKALNLPNQDRKDSQGICFLGKIKFSEFVARHIGEEEGPILEAETG 673
            FPLG +PKDEVR LA   NLPN+DRKDSQGICFLGKIKFSEFVARHIGE+EG ILEAE+G
Sbjct: 251  FPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESG 310

Query: 672  DLLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRLFRVGSL 493
            D LG HRGFWFYTIGQRQGLRLPGGPWYVVEKD+KNNVVFVSRNY+S DKRRR+FRVGSL
Sbjct: 311  DFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSL 370

Query: 492  KWISGFSPAQVNEIQCKVRHGPGFYNCTLT-XXXXXXXXXXXXVQLAEDDQGLAAGQFAA 316
            KW+SGF P Q  E+QCKVRHGP FY+C LT             V L+EDDQGLAAGQFAA
Sbjct: 371  KWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAA 430

Query: 315  FYKSDTCIGSGVILESWDDQGFPLCARALEMAIMEDKSKLGKPVKIKNKPESISEES 145
            FYK  +CIGSGVILESWDDQGFP+C +A+E A MEDKSKLGKP+KIK KPES  +ES
Sbjct: 431  FYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKES 487


>ref|XP_003539065.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Glycine max]
          Length = 488

 Score =  664 bits (1713), Expect = 0.0
 Identities = 320/419 (76%), Positives = 353/419 (84%), Gaps = 1/419 (0%)
 Frame = -2

Query: 1395 KYLCCSMADKRPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECP 1216
            +YL CSM    PL+VAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECP
Sbjct: 50   RYLRCSMPQNSPLRVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECP 109

Query: 1215 WETDLNYARAVCDQVDVALEVVHLTDEYWKNVVSYIIAEYRSGRTPNPDVLCNTRIKFGA 1036
            WE DL YA+ VC+QVDV LEVVHLTDEYW NVVSY+I EY SGRTPNPDVLCNTRIKFGA
Sbjct: 110  WEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVSYLIEEYSSGRTPNPDVLCNTRIKFGA 169

Query: 1035 FMDAISAMEFDYVASGHYAKVMHSSLLDSDEPSILQLSKDMVKDQTYFLSHLSPEQLKRL 856
            F+DAI  M FDYVASGHYA V+H      DEPS+L+LS+DMVKDQTYFLSHLS  QLK+L
Sbjct: 170  FLDAIGGMGFDYVASGHYANVIHPCSDRRDEPSVLELSQDMVKDQTYFLSHLSQSQLKQL 229

Query: 855  IFPLGSMPKDEVRGLAKALNLPNQDRKDSQGICFLGKIKFSEFVARHIGEEEGPILEAET 676
            +FPLG +PKDEVR LA   +LPN+DRKDSQGICFLGKI+FSEFVARHIGE EG ILEAET
Sbjct: 230  LFPLGCIPKDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILEAET 289

Query: 675  GDLLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRLFRVGS 496
            GD LG HRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+S DKRRR+FRVGS
Sbjct: 290  GDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGS 349

Query: 495  LKWISGFSPAQVNEIQCKVRHGPGFYNCTL-TXXXXXXXXXXXXVQLAEDDQGLAAGQFA 319
             KW+SG  P Q  ++QCKVRHGPGFY+C+L              V+++EDDQGLAAGQFA
Sbjct: 350  FKWLSGLPPGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSAVVRISEDDQGLAAGQFA 409

Query: 318  AFYKSDTCIGSGVILESWDDQGFPLCARALEMAIMEDKSKLGKPVKIKNKPESISEESD 142
            AFY+  TCIGSGVILE WDDQ FP+C +ALE+A MEDKSK+G PVKIK KP++  E  D
Sbjct: 410  AFYEGRTCIGSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPVKIKVKPDNPQEVCD 468