BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00014070
         (1963 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265630.1| PREDICTED: ent-kaurenoic acid oxidase 2 [Vit...   732   0.0  
dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]           710   0.0  
gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]       709   0.0  
ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinu...   707   0.0  
ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid ...   706   0.0  

>ref|XP_002265630.1| PREDICTED: ent-kaurenoic acid oxidase 2 [Vitis vinifera]
          Length = 492

 Score =  732 bits (1890), Expect = 0.0
 Identities = 353/491 (71%), Positives = 412/491 (83%)
 Frame = +3

Query: 213  MDLKLNWNMLMVILGGFVTMKWVLKSINMWYYEKYKLGGDKKYSLLPPGDMGWPLIGNMW 392
            M+L + W     ILGG + +KWVL+  N W YE  KLG +K+YSL PPGD+GWPLIGNMW
Sbjct: 1    MELGMIWVAFGAILGGVLGVKWVLRRANSWVYE-VKLG-EKRYSL-PPGDLGWPLIGNMW 57

Query: 393  SFLLAFKSSNPDSFISSFVTRFGQIGLYKTFMFGHPSVIVTTPEACKRALTDDEDFKPGW 572
            SFL AFKS++PDSFISSF+TRFGQ G+YK  MFG+PS+IVT PEACKR LTDD++FKPGW
Sbjct: 58   SFLRAFKSTDPDSFISSFITRFGQTGMYKVLMFGNPSIIVTIPEACKRVLTDDQNFKPGW 117

Query: 573  PSSTLKLIGRKSFIGITYEEHKRLRKLTAAPVNGHEALSMYTKYIEEIVISSLEKWSSMG 752
            P+ST++LIGRKSFIGIT EEHKRLR+LTA PVNGHEALS+Y +YIE+ VIS+L KW++MG
Sbjct: 118  PTSTMELIGRKSFIGITNEEHKRLRRLTATPVNGHEALSIYMQYIEDNVISALNKWAAMG 177

Query: 753  KIEFLTEMRRLTFKIIMYIFLSSESEHVMEALEREYTTLNYGVRAMAINIPGFXXXXXXX 932
            + EFLT +R+LTFKIIMYIFLSSESEHVMEALEREYT+LNYGVR+MAIN+PGF       
Sbjct: 178  EFEFLTALRKLTFKIIMYIFLSSESEHVMEALEREYTSLNYGVRSMAINLPGFAYHKALK 237

Query: 933  XXXXXXXIFQSIVDERRDRRRKNPTGEKKDMMDILLDEQDEKGRKLNDEEIIDVLVMYLN 1112
                   IFQSIV+ERRDR++ N    KKDMMD LLD +DE GRKL+DEEIID+LVMYLN
Sbjct: 238  ARKNLVNIFQSIVNERRDRKKGNSQTMKKDMMDALLDIEDENGRKLSDEEIIDILVMYLN 297

Query: 1113 AGHESSGHITMWATLLLQKHPEFFKRAKAEQEEIVMNRPPGQVGLTLKETRKMEYLSKVI 1292
            AGHESS H+TMWAT+ LQ++PEFF+RAKAEQEEI+  RPP Q  LTLKE R+MEYL KVI
Sbjct: 298  AGHESSAHVTMWATVKLQENPEFFQRAKAEQEEIIRKRPPNQKRLTLKEIREMEYLPKVI 357

Query: 1293 DETLRLVTFSFVVFREAKRDVRLCGYTIPKGWKLLLWFRSVHLDPEIYPSPKEFNPSRWD 1472
            DETLR +TFSFVVFREAK D+ +CGYTIPKGWK+L+WFRS+H DPE YP PKEFNP RWD
Sbjct: 358  DETLRWITFSFVVFREAKADINICGYTIPKGWKVLVWFRSLHFDPETYPDPKEFNPCRWD 417

Query: 1473 ELTPKAGTFLPFGGGSRMCPGNDLAKLEISIFLHYFLLNYELERENNACPVMNLPHSRPK 1652
            + T K GTFLPFG GSR+CPGNDLAKLEIS+FLH+FLLNY+LER N  CP M LPHSRP+
Sbjct: 418  DYTAKPGTFLPFGLGSRLCPGNDLAKLEISVFLHHFLLNYQLERLNPGCPRMYLPHSRPR 477

Query: 1653 DNCLGRVRRVS 1685
            DNCL  VR+V+
Sbjct: 478  DNCLAIVRKVA 488


>dbj|BAG71199.1| ent-kaurenoic acid oxidase [Lactuca sativa]
          Length = 493

 Score =  710 bits (1833), Expect = 0.0
 Identities = 342/480 (71%), Positives = 396/480 (82%)
 Frame = +3

Query: 246  VILGGFVTMKWVLKSINMWYYEKYKLGGDKKYSLLPPGDMGWPLIGNMWSFLLAFKSSNP 425
            + LG  + +KWVLKS+N+W YE+      KK   LPPGDMGWP IGNMWSFL AFKS++P
Sbjct: 15   IFLGLMLILKWVLKSVNIWIYERNL--EKKKRKCLPPGDMGWPFIGNMWSFLRAFKSNDP 72

Query: 426  DSFISSFVTRFGQIGLYKTFMFGHPSVIVTTPEACKRALTDDEDFKPGWPSSTLKLIGRK 605
            D+FIS+F+ RFG  GLYK+FMFG PS+IVT PEAC++ L DDE F+PGWP++T +LIG+K
Sbjct: 73   DAFISNFLRRFGSRGLYKSFMFGSPSIIVTVPEACRKVLFDDEAFQPGWPTATRELIGKK 132

Query: 606  SFIGITYEEHKRLRKLTAAPVNGHEALSMYTKYIEEIVISSLEKWSSMGKIEFLTEMRRL 785
            SFIGI+YE+HKRLRKLTAAPVNGHEALS Y +YIE  V+ +LEKWS MG+IEFLT +R+L
Sbjct: 133  SFIGISYEDHKRLRKLTAAPVNGHEALSTYMQYIETNVVLALEKWSKMGRIEFLTYLRKL 192

Query: 786  TFKIIMYIFLSSESEHVMEALEREYTTLNYGVRAMAINIPGFXXXXXXXXXXXXXXIFQS 965
            TF+IIM+IFLS+ESEHVM+ALE+EYT LNYGVRAMAINIPGF              I Q+
Sbjct: 193  TFQIIMHIFLSTESEHVMDALEKEYTVLNYGVRAMAINIPGFAYYNALKARKNLVAILQT 252

Query: 966  IVDERRDRRRKNPTGEKKDMMDILLDEQDEKGRKLNDEEIIDVLVMYLNAGHESSGHITM 1145
            +V ERR +R  N    KKDM+D LLD  DEKGRKL+DEEIID LVMYLNAGHESSGHITM
Sbjct: 253  VVYERRKKREANEGMSKKDMLDALLDTTDEKGRKLDDEEIIDTLVMYLNAGHESSGHITM 312

Query: 1146 WATLLLQKHPEFFKRAKAEQEEIVMNRPPGQVGLTLKETRKMEYLSKVIDETLRLVTFSF 1325
            W+T+LLQ HPEFF  AK EQE IV N PP Q GLTLKE R+MEYLSKVIDETLRLVTFS 
Sbjct: 313  WSTILLQAHPEFFHIAKEEQERIVKNMPPTQEGLTLKEYRQMEYLSKVIDETLRLVTFSL 372

Query: 1326 VVFREAKRDVRLCGYTIPKGWKLLLWFRSVHLDPEIYPSPKEFNPSRWDELTPKAGTFLP 1505
            + FREAK+DV + GY IPKGWK+LLWFRSVH +PE+YP PKEFNPSRWD+L PK GTFLP
Sbjct: 373  MTFREAKKDVDIKGYFIPKGWKVLLWFRSVHHNPELYPQPKEFNPSRWDDLVPKPGTFLP 432

Query: 1506 FGGGSRMCPGNDLAKLEISIFLHYFLLNYELERENNACPVMNLPHSRPKDNCLGRVRRVS 1685
            FG GSR+CPGNDLAKLEI+IFLH+FL+NYE EREN  CP+M LPHSRPKDNC+GRVRRVS
Sbjct: 433  FGAGSRLCPGNDLAKLEIAIFLHHFLVNYEFERENPECPIMYLPHSRPKDNCVGRVRRVS 492


>gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]
          Length = 492

 Score =  709 bits (1830), Expect = 0.0
 Identities = 341/489 (69%), Positives = 405/489 (82%)
 Frame = +3

Query: 213  MDLKLNWNMLMVILGGFVTMKWVLKSINMWYYEKYKLGGDKKYSLLPPGDMGWPLIGNMW 392
            M+L    N+L+ I G  V +KWV+K+ N W YE     G+K+YSL PPGD+GWP IGNMW
Sbjct: 1    MELGPICNVLLCIFGVLVVLKWVVKNANWWLYETQL--GEKQYSL-PPGDLGWPFIGNMW 57

Query: 393  SFLLAFKSSNPDSFISSFVTRFGQIGLYKTFMFGHPSVIVTTPEACKRALTDDEDFKPGW 572
            SFL AFKS +PDSF+SSFV+RFG+ G+YK FMFG+PSVIVT+PEAC+R L+DD+ FKPGW
Sbjct: 58   SFLSAFKSKDPDSFVSSFVSRFGRTGIYKVFMFGNPSVIVTSPEACRRVLSDDDCFKPGW 117

Query: 573  PSSTLKLIGRKSFIGITYEEHKRLRKLTAAPVNGHEALSMYTKYIEEIVISSLEKWSSMG 752
            P ST+ LIG+KSFIGI++EEHKRLR+LTAAPVNG+EALSMYTKYIEEIV SSL+KW++MG
Sbjct: 118  PKSTVALIGKKSFIGISFEEHKRLRRLTAAPVNGYEALSMYTKYIEEIVTSSLDKWTTMG 177

Query: 753  KIEFLTEMRRLTFKIIMYIFLSSESEHVMEALEREYTTLNYGVRAMAINIPGFXXXXXXX 932
            +IEFLTE+R+LTF+IIMYIFLSSESE VM ALEREYT LNYGVRAMAINIPGF       
Sbjct: 178  EIEFLTELRKLTFRIIMYIFLSSESEPVMTALEREYTALNYGVRAMAINIPGFAYHKALK 237

Query: 933  XXXXXXXIFQSIVDERRDRRRKNPTGEKKDMMDILLDEQDEKGRKLNDEEIIDVLVMYLN 1112
                    FQSIVD+RR++++ +   +KKDMMD L+D +DE GRKL DEEIID+LVMYLN
Sbjct: 238  ARKNLVATFQSIVDDRRNQKKVSVPTKKKDMMDALIDVKDENGRKLTDEEIIDILVMYLN 297

Query: 1113 AGHESSGHITMWATLLLQKHPEFFKRAKAEQEEIVMNRPPGQVGLTLKETRKMEYLSKVI 1292
            AGHESSGH  MWAT+ L +HPE+ ++AK EQEEIV  R P Q GLTLKE R+MEYLSKVI
Sbjct: 298  AGHESSGHTAMWATIFLNQHPEYLQKAKKEQEEIVRKRLPEQKGLTLKEIREMEYLSKVI 357

Query: 1293 DETLRLVTFSFVVFREAKRDVRLCGYTIPKGWKLLLWFRSVHLDPEIYPSPKEFNPSRWD 1472
            DETLR++TFS  VFREAK+DV + GYTIPKGW++L+WFRSVHLDPEIY +PKEF PSRWD
Sbjct: 358  DETLRVITFSLTVFREAKQDVNIAGYTIPKGWRVLVWFRSVHLDPEIYENPKEFKPSRWD 417

Query: 1473 ELTPKAGTFLPFGGGSRMCPGNDLAKLEISIFLHYFLLNYELERENNACPVMNLPHSRPK 1652
              TPKAG FLPFG G+R+CPGNDLAKLEISIFLH+FLLNY+LER N   P M LPH+RP 
Sbjct: 418  NFTPKAGAFLPFGAGTRLCPGNDLAKLEISIFLHHFLLNYQLERLNPGSPSMYLPHTRPV 477

Query: 1653 DNCLGRVRR 1679
            DNCL R+++
Sbjct: 478  DNCLARIKK 486


>ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinus communis]
            gi|223534199|gb|EEF35915.1| Ent-kaurenoic acid oxidase,
            putative [Ricinus communis]
          Length = 492

 Score =  707 bits (1824), Expect = 0.0
 Identities = 339/492 (68%), Positives = 406/492 (82%), Gaps = 2/492 (0%)
 Frame = +3

Query: 213  MDLKLNWNMLMVILGGFVTMKWVLKSINMWYYEKYKLGGDKKYSLLPPGDMGWPLIGNMW 392
            M++   W +L+ I GGF  +KW+LK +N W YE     G+ +YSL PPGD+GWP IGNMW
Sbjct: 1    MEMGFVWVVLIWISGGFWCLKWILKRVNCWLYENQL--GEMQYSL-PPGDLGWPFIGNMW 57

Query: 393  SFLLAFKSSNPDSFISSFVTRFGQIGLYKTFMFGHPSVIVTTPEACKRALTDDEDFKPGW 572
            SFL AFKS++PDSF+ +F  R+G  G+YK FMFG PSV+VTT EACKR LTDD+ FKPGW
Sbjct: 58   SFLRAFKSNDPDSFMRNFTARYGSGGIYKAFMFGKPSVLVTTSEACKRVLTDDDAFKPGW 117

Query: 573  PSSTLKLIGRKSFIGITYEEHKRLRKLTAAPVNGHEALSMYTKYIEEIVISSLEKWSSMG 752
            PSST +LIG+KSFIGI+YEEHKRLR+LTA+PVNGHEALS+Y  YIE+ V S+LEKWS+MG
Sbjct: 118  PSSTTELIGKKSFIGISYEEHKRLRRLTASPVNGHEALSVYMHYIEDKVKSALEKWSTMG 177

Query: 753  KIEFLTEMRRLTFKIIMYIFLSSESEHVMEALEREYTTLNYGVRAMAINIPGFXXXXXXX 932
            +I+FLT++R+LTF+IIMYIFLSSES  VMEALEREYTTLNYGVRAMAIN+PGF       
Sbjct: 178  EIQFLTQLRKLTFRIIMYIFLSSESHSVMEALEREYTTLNYGVRAMAINLPGFAYYKALK 237

Query: 933  XXXXXXXIFQSIVDERRDRRR--KNPTGEKKDMMDILLDEQDEKGRKLNDEEIIDVLVMY 1106
                   + Q +VD RR++++  +  + +KKDMMD LLD +DEKGRKL+DEEI+DVL+MY
Sbjct: 238  ARKNLVAVLQFVVDARRNQKKGAEPNSSKKKDMMDALLDVEDEKGRKLSDEEIVDVLLMY 297

Query: 1107 LNAGHESSGHITMWATLLLQKHPEFFKRAKAEQEEIVMNRPPGQVGLTLKETRKMEYLSK 1286
            LNAGHESSGHITMWAT+ LQ+HPEF ++A+ EQEEI+  RPP Q GLTLKE R MEYLSK
Sbjct: 298  LNAGHESSGHITMWATVFLQEHPEFLQKARQEQEEIIKRRPPTQKGLTLKEVRDMEYLSK 357

Query: 1287 VIDETLRLVTFSFVVFREAKRDVRLCGYTIPKGWKLLLWFRSVHLDPEIYPSPKEFNPSR 1466
            VIDETLRL+TFS VVFREAK +V + GY IPKGWK+L+WFRSVHLDPEIYP+P+EFNPSR
Sbjct: 358  VIDETLRLITFSLVVFREAKTNVNISGYVIPKGWKILVWFRSVHLDPEIYPNPREFNPSR 417

Query: 1467 WDELTPKAGTFLPFGGGSRMCPGNDLAKLEISIFLHYFLLNYELERENNACPVMNLPHSR 1646
            WD  T KAGTFLPFG GSRMCPGNDLAKLEI+IFLH+FLLNYELER N    +M LPHSR
Sbjct: 418  WDNHTAKAGTFLPFGAGSRMCPGNDLAKLEIAIFLHHFLLNYELERLNPGSSMMYLPHSR 477

Query: 1647 PKDNCLGRVRRV 1682
            PKDNCL R++++
Sbjct: 478  PKDNCLARIKKI 489


>ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222862021|gb|EEE99563.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 493

 Score =  706 bits (1823), Expect = 0.0
 Identities = 342/490 (69%), Positives = 401/490 (81%), Gaps = 1/490 (0%)
 Frame = +3

Query: 231  WNMLMVILGGFVTMKWVLKSINMWYYEKYKLGGDKKYSLLPPGDMGWPLIGNMWSFLLAF 410
            W +L VI GG    KW+LK +N W YE     G+K+YSL PPGD+GWP IGNMWSFL AF
Sbjct: 7    WVVLAVIFGGLGVGKWILKKVNWWLYEAQL--GEKQYSL-PPGDLGWPFIGNMWSFLRAF 63

Query: 411  KSSNPDSFISSFVTRFGQIGLYKTFMFGHPSVIVTTPEACKRALTDDEDFKPGWPSSTLK 590
            KSS+PDSF+ +F+ ++G  G+YK FMFG+PSV VTTPEAC+R L++D+ FKPGWP STLK
Sbjct: 64   KSSDPDSFMRTFINKYGDNGIYKAFMFGNPSVFVTTPEACRRVLSNDDAFKPGWPISTLK 123

Query: 591  LIGRKSFIGITYEEHKRLRKLTAAPVNGHEALSMYTKYIEEIVISSLEKWSSMGKIEFLT 770
            LIGRKSFI I+YEEHKRLR+LT+APVNGHEALS+Y  YIEE VI+ LEKW++MGKIEFLT
Sbjct: 124  LIGRKSFIDISYEEHKRLRRLTSAPVNGHEALSVYIPYIEENVIAMLEKWTTMGKIEFLT 183

Query: 771  EMRRLTFKIIMYIFLSSESEHVMEALEREYTTLNYGVRAMAINIPGFXXXXXXXXXXXXX 950
            ++R+LTFKIIMYIFLSSESE VMEALE++YTTLNYGVRAMAIN+PGF             
Sbjct: 184  QVRKLTFKIIMYIFLSSESEVVMEALEKDYTTLNYGVRAMAINLPGFAYYKALKARKRLV 243

Query: 951  XIFQSIVDERRDRRRKNP-TGEKKDMMDILLDEQDEKGRKLNDEEIIDVLVMYLNAGHES 1127
             IFQSIVDERR+ R+ +    +KKDMMD LL  +DE GRKL DEEIIDV++MYLNAGHES
Sbjct: 244  AIFQSIVDERRNLRKNSARNAKKKDMMDSLLGVEDENGRKLTDEEIIDVILMYLNAGHES 303

Query: 1128 SGHITMWATLLLQKHPEFFKRAKAEQEEIVMNRPPGQVGLTLKETRKMEYLSKVIDETLR 1307
            SGHIT WAT+ LQ+HPEF ++AK EQE+IV  RPP Q GL+LKE R+M+YLSKVIDETLR
Sbjct: 304  SGHITTWATIFLQEHPEFLQKAKEEQEQIVKRRPPAQNGLSLKEVREMDYLSKVIDETLR 363

Query: 1308 LVTFSFVVFREAKRDVRLCGYTIPKGWKLLLWFRSVHLDPEIYPSPKEFNPSRWDELTPK 1487
            L+TFS  VFREAK D  + GY IPKGWK+L+WFR+VHLDPEIY +PKEFNPSRWD  TPK
Sbjct: 364  LITFSLTVFREAKTDFSINGYIIPKGWKVLVWFRTVHLDPEIYQNPKEFNPSRWDNYTPK 423

Query: 1488 AGTFLPFGGGSRMCPGNDLAKLEISIFLHYFLLNYELERENNACPVMNLPHSRPKDNCLG 1667
            AGTFLPFG GSR+CPGNDLAKLEISIFLHYFLL+Y LEREN  C  M LPH+RP DNC+ 
Sbjct: 424  AGTFLPFGAGSRLCPGNDLAKLEISIFLHYFLLDYRLERENPECRWMFLPHTRPTDNCVA 483

Query: 1668 RVRRVSPYGV 1697
            R+++VS   V
Sbjct: 484  RIKKVSSTSV 493