BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00013493
         (1984 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desatur...   840   0.0  
gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    736   0.0  
ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-li...   721   0.0  
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        719   0.0  
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   717   0.0  

>sp|Q9FR82.1|SLD1_BOROF RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
            Full=Delta(8)-sphingolipid desaturase
            gi|12002282|gb|AAG43277.1|AF133728_1 delta 8-sphingolipid
            desaturase [Borago officinalis]
          Length = 446

 Score =  840 bits (2170), Expect = 0.0
 Identities = 394/446 (88%), Positives = 417/446 (93%)
 Frame = -3

Query: 1565 MESTKKYISLDELEKHNKHEDVWISIEGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 1386
            ME TKKYIS+ ELEKHN+  DVWISI+GKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI
Sbjct: 1    MEGTKKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 60

Query: 1385 AYHPGTAWKYLENLFTGYYLEDYQVSELSRDYRKLASEFSRAGLFEKKGHTVIYCFSFVA 1206
            AYHPGTAWK LENLFTGY+LEDY VSE+S+DYRKLASEFS+AGLFEKKGHTVIYC SF+A
Sbjct: 61   AYHPGTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIA 120

Query: 1205 LLLFGCFYGVLCSDNFWVHMLCGAMLGMCWIQAAYLGHDSGHYTMMSTKGYNKLAQILNG 1026
            LLL GC YGVLCS++ WVHML GAMLGMC+IQAAYLGHDSGHYTMMS+KGYNK AQ+LNG
Sbjct: 121  LLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSKGYNKFAQVLNG 180

Query: 1025 NLLTGISIAWWNWTHNAHHIACNSLDYDPDLQHLPVFAVSPSFFKSLTSVFYGRELTFDG 846
            N LTGISIAWW WTHNAHHIACNSLDYDPDLQHLPVFAV  SFFKSLTS FYGRELTFDG
Sbjct: 181  NCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPVFAVPSSFFKSLTSRFYGRELTFDG 240

Query: 845  LSRFFVSYQHFTYYPVMIFGRVNLYVQTFLLLFSTRKVNDRALNISGILIFWTWFPYLVS 666
            LSRF VSYQHFT Y VMIFGR+NLYVQTFLLLFSTRKV DRALNI GIL++WTWFPYLVS
Sbjct: 241  LSRFLVSYQHFTIYLVMIFGRINLYVQTFLLLFSTRKVPDRALNIIGILVYWTWFPYLVS 300

Query: 665  CLPNWNERVLFIFTSFSVTALQHIQFTLNHFAANVYVGAPTGNDWFEKQTAGTIDISCSS 486
            CLPNWNERVLF+ T FSVTALQHIQFTLNHFAA+VYVG PTG +WFEKQ AGTIDISCSS
Sbjct: 301  CLPNWNERVLFVLTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSS 360

Query: 485  YMDWFFGGLQFQLEHHLFPRMPRCQLRNIAPIIQEYCKKHNLPYRSLSFFDANVATIKTL 306
            +MDWFFGGLQFQLEHHLFPRMPRCQLRNI+PI+Q+YCKKHNLPYRSLSFFDANVATIKTL
Sbjct: 361  WMDWFFGGLQFQLEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTL 420

Query: 305  RTAALQARDLTTVPQNLLWEAVNTHG 228
            RTAALQARDLT VPQNLLWEA NTHG
Sbjct: 421  RTAALQARDLTVVPQNLLWEAFNTHG 446


>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  736 bits (1901), Expect = 0.0
 Identities = 336/443 (75%), Positives = 383/443 (86%), Gaps = 1/443 (0%)
 Frame = -3

Query: 1553 KKYISLDELEKHNKHEDVWISIEGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFIAYHP 1374
            KKYI+ DEL+KHNK  D+WISI+GKVYNVT+W K HPGG++P+MNLAGQD TDAFIA+HP
Sbjct: 24   KKYITSDELKKHNKPNDLWISIQGKVYNVTEWAKIHPGGEIPLMNLAGQDVTDAFIAFHP 83

Query: 1373 GTAWKYLENLFTGYYLEDYQVSELSRDYRKLASEFSRAGLFEKKGHTVIYCFSFVALLLF 1194
            G+AW++L  LFTGY+L+DYQVS +S+DYRKLASEF++AG+FEKKGH VIY   FV+LLL 
Sbjct: 84   GSAWEHLSKLFTGYHLKDYQVSAVSKDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 143

Query: 1193 GCFYGVLCSDNFWVHMLCGAMLGMCWIQAAYLGHDSGHYTMMSTKGYNKLAQILNGNLLT 1014
            GC YGVL S +FWVHML GA+LG+ WIQ AYLGHD+GHY MMST+G+NK A I  GN +T
Sbjct: 144  GCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDAGHYQMMSTRGWNKFAGIFIGNCIT 203

Query: 1013 GISIAWWNWTHNAHHIACNSLDYDPDLQHLPVFAVSPSFFKSLTSVFYGRELTFDGLSRF 834
            GISIAWW WTHNAHHIACNSLDYDPDLQHLP+ AVS   F SLTSVFYGR+LTFD L+RF
Sbjct: 204  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSRLFNSLTSVFYGRQLTFDRLARF 263

Query: 833  FVSYQHFTYYPVMIFGRVNLYVQTFLLLFSTRKVNDRALNISGILIFWTWFPYLVSCLPN 654
            FVSYQH++YYP+M   RVNLY+QT LLL S RKV D+ LNI G L+FWTWFP LVSCLPN
Sbjct: 264  FVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPDKGLNIFGTLVFWTWFPLLVSCLPN 323

Query: 653  WNERVLFIFTSFSVTALQHIQFTLNHFAANVYVGAPTGNDWFEKQTAGTIDISCSSYMDW 474
            W ERV F+  SF VT +QH+QFTLNHFAANVYVG PTGNDWFEKQT GTIDI+CSSYMDW
Sbjct: 324  WPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGPPTGNDWFEKQTNGTIDIACSSYMDW 383

Query: 473  FFGGLQFQLEHHLFPRMPRCQLRNIAPIIQEYCKKHNLPYRSLSFFDANVATIKTLRTAA 294
            FFGGLQFQLEHHLFPR+PRC LR+IAPI++E CKKHNLPY S+SFFDAN+ T+KTLRTAA
Sbjct: 384  FFGGLQFQLEHHLFPRLPRCHLRSIAPIVRELCKKHNLPYTSVSFFDANIMTLKTLRTAA 443

Query: 293  LQARDLTT-VPQNLLWEAVNTHG 228
            LQARDLT   PQNLLWEA NTHG
Sbjct: 444  LQARDLTNPAPQNLLWEAFNTHG 466


>ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
            sativus] gi|449441408|ref|XP_004138474.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
            sativus] gi|449495238|ref|XP_004159774.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
            sativus] gi|449495242|ref|XP_004159775.1| PREDICTED:
            delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
            sativus]
          Length = 447

 Score =  721 bits (1860), Expect = 0.0
 Identities = 334/447 (74%), Positives = 388/447 (86%), Gaps = 1/447 (0%)
 Frame = -3

Query: 1565 MESTKKYISLDELEKHNKHEDVWISIEGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 1386
            ME  KKYI+ +EL KHNK  D+WISI+GKVYNVT+W K+HPGGD P +NLAG D TDAFI
Sbjct: 1    MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 1385 AYHPGTAWKYLENLFTGYYLEDYQVSELSRDYRKLASEFSRAGLFEKKGHTVIYCFSFVA 1206
            AYHPGTAW YL+ LFTG++L+D+QVSE+S+DYRKLASEFS+ GLFEKKGH  +Y    VA
Sbjct: 61   AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 1205 LLLFGCFYGVLCSDNFWVHMLCGAMLGMCWIQAAYLGHDSGHYTMMSTKGYNKLAQILNG 1026
            +++F   YGVL S++  VH+ C  +LGM WIQ+AY+GHDSGH  +MS++G+NKLAQIL+G
Sbjct: 121  VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 1025 NLLTGISIAWWNWTHNAHHIACNSLDYDPDLQHLPVFAVSPSFFKSLTSVFYGRELTFDG 846
            N LTGISIAWW WTHNAHHI+CNSLD+DPDLQH+PVFAVS SFF++LTS FYGR+L FD 
Sbjct: 181  NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 845  LSRFFVSYQHFTYYPVMIFGRVNLYVQTFLLLFSTRKVNDRALNISGILIFWTWFPYLVS 666
            L+RFFVSYQHFT+YPVM   RVNL+VQT LLLFSTRKV DRALNI GIL+FWTWFP LVS
Sbjct: 241  LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 665  CLPNWNERVLFIFTSFSVTALQHIQFTLNHFAANVYVGAPTGNDWFEKQTAGTIDISCSS 486
             LPNW ERV+F+  SF+V +LQHIQF LNHF+ANVYVG PTGNDWFEKQ +GT+DISCS+
Sbjct: 301  YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 485  YMDWFFGGLQFQLEHHLFPRMPRCQLRNIAPIIQEYCKKHNLPYRSLSFFDANVATIKTL 306
            +MDWFFGGLQFQLEHHLFPRMPRCQLR I+PI+ E CKKHNLPYRSLSF+DANV TI+TL
Sbjct: 361  WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 305  RTAALQARDLTT-VPQNLLWEAVNTHG 228
            RTAALQARDLT  VP+NLLWEAVNTHG
Sbjct: 421  RTAALQARDLTNPVPKNLLWEAVNTHG 447


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  719 bits (1855), Expect = 0.0
 Identities = 326/443 (73%), Positives = 380/443 (85%), Gaps = 1/443 (0%)
 Frame = -3

Query: 1553 KKYISLDELEKHNKHEDVWISIEGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFIAYHP 1374
            KKYI+ +ELEKHNK  D+WISI+GKVYNVT+W K HPGGD+P++NLAGQD TDAFIA+HP
Sbjct: 27   KKYITSEELEKHNKPTDLWISIQGKVYNVTEWSKIHPGGDIPLLNLAGQDVTDAFIAFHP 86

Query: 1373 GTAWKYLENLFTGYYLEDYQVSELSRDYRKLASEFSRAGLFEKKGHTVIYCFSFVALLLF 1194
            G+AW +L++LFTGY+L+DYQVS++S+DYRKLASEFS+AG+F+KKGH VIY   FV++LL 
Sbjct: 87   GSAWHHLQSLFTGYHLQDYQVSDISKDYRKLASEFSKAGMFDKKGHGVIYSLCFVSILLS 146

Query: 1193 GCFYGVLCSDNFWVHMLCGAMLGMCWIQAAYLGHDSGHYTMMSTKGYNKLAQILNGNLLT 1014
             C YGVLCS +  VHM  GA+LG+ W+Q AYLGHD+GHY MM+T+G+NK A I  GN +T
Sbjct: 147  LCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 206

Query: 1013 GISIAWWNWTHNAHHIACNSLDYDPDLQHLPVFAVSPSFFKSLTSVFYGRELTFDGLSRF 834
            GISIAWW WTHNAHHIACNSLDYDPDLQHLP+ AVS   F+S+TSVFYGR LTFD ++RF
Sbjct: 207  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFESITSVFYGRRLTFDSVARF 266

Query: 833  FVSYQHFTYYPVMIFGRVNLYVQTFLLLFSTRKVNDRALNISGILIFWTWFPYLVSCLPN 654
            F+SYQH+TYYP+M   RVNLY+QT LLL S R++ DR LNI G LIFWTWFP LVSCLPN
Sbjct: 267  FISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRIPDRTLNIIGTLIFWTWFPLLVSCLPN 326

Query: 653  WNERVLFIFTSFSVTALQHIQFTLNHFAANVYVGAPTGNDWFEKQTAGTIDISCSSYMDW 474
            W ERV F+  SF VT +QH+QFTLNHFAA+VYVG P GNDWFEKQTAGTIDISCSSYMDW
Sbjct: 327  WPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGPPRGNDWFEKQTAGTIDISCSSYMDW 386

Query: 473  FFGGLQFQLEHHLFPRMPRCQLRNIAPIIQEYCKKHNLPYRSLSFFDANVATIKTLRTAA 294
            FFGGLQFQLEHHLFPR+PRC LR+I+PI+ + CKKHNLPY SLSFFDANV T+KTLRTAA
Sbjct: 387  FFGGLQFQLEHHLFPRLPRCHLRSISPIVMDLCKKHNLPYTSLSFFDANVTTLKTLRTAA 446

Query: 293  LQARDLTT-VPQNLLWEAVNTHG 228
            LQARDL    PQNL+WEA NTHG
Sbjct: 447  LQARDLANGPPQNLVWEAFNTHG 469


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  717 bits (1851), Expect = 0.0
 Identities = 319/447 (71%), Positives = 385/447 (86%), Gaps = 1/447 (0%)
 Frame = -3

Query: 1565 MESTKKYISLDELEKHNKHEDVWISIEGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 1386
            M   +KYIS +EL++H+K  D+WISI+GKVYNVTDW K HPGGD+P++NLAGQD TDAFI
Sbjct: 1    MAGERKYISAEELKQHDKPGDLWISIQGKVYNVTDWAKVHPGGDIPLLNLAGQDVTDAFI 60

Query: 1385 AYHPGTAWKYLENLFTGYYLEDYQVSELSRDYRKLASEFSRAGLFEKKGHTVIYCFSFVA 1206
            AYHPGTAW+YL+  FTGYYL+D++VSE+S+D+RKLA+EFS+ GLFEKKGH ++Y  S + 
Sbjct: 61   AYHPGTAWQYLDKFFTGYYLKDFKVSEVSKDFRKLANEFSKLGLFEKKGHGILYTCSIIG 120

Query: 1205 LLLFGCFYGVLCSDNFWVHMLCGAMLGMCWIQAAYLGHDSGHYTMMSTKGYNKLAQILNG 1026
             L   C YGVLC D+FWVH+ C A+LG  WIQ++YLGHDSGHY +MS+ G+NKLAQ+L G
Sbjct: 121  FLFMVCIYGVLCVDSFWVHLGCAALLGFLWIQSSYLGHDSGHYQIMSSPGFNKLAQMLIG 180

Query: 1025 NLLTGISIAWWNWTHNAHHIACNSLDYDPDLQHLPVFAVSPSFFKSLTSVFYGRELTFDG 846
            N +TGISIAWW WTHNAHHIACNSLDYDPDLQH+PVFAVS S F S+ SVFYGR L FD 
Sbjct: 181  NCITGISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAVSSSLFDSINSVFYGRILKFDS 240

Query: 845  LSRFFVSYQHFTYYPVMIFGRVNLYVQTFLLLFSTRKVNDRALNISGILIFWTWFPYLVS 666
            ++RFFVSYQH+TYYPVM   R+NL++QT LLLFS R + DRALNI GI +FWTW+P LVS
Sbjct: 241  VARFFVSYQHWTYYPVMCVARINLFLQTLLLLFSGRNIPDRALNIMGICVFWTWYPLLVS 300

Query: 665  CLPNWNERVLFIFTSFSVTALQHIQFTLNHFAANVYVGAPTGNDWFEKQTAGTIDISCSS 486
            CLPNW ERV+F+  S +VT++QHIQFTLNHF+ANVYVG P GNDWFEKQT+GTIDISCS+
Sbjct: 301  CLPNWGERVMFVIVSMAVTSIQHIQFTLNHFSANVYVGPPCGNDWFEKQTSGTIDISCSA 360

Query: 485  YMDWFFGGLQFQLEHHLFPRMPRCQLRNIAPIIQEYCKKHNLPYRSLSFFDANVATIKTL 306
            +MDWF GGLQFQLEHHLFPR+PRC LR+I+P++++ CKKHNLPYRSLSF++ANV+TI+TL
Sbjct: 361  WMDWFHGGLQFQLEHHLFPRLPRCHLRSISPVVKDLCKKHNLPYRSLSFWEANVSTIRTL 420

Query: 305  RTAALQARDLTT-VPQNLLWEAVNTHG 228
            RTAALQARDLT  VP+NL+WEA N HG
Sbjct: 421  RTAALQARDLTNPVPKNLVWEAANIHG 447