BLASTX nr result
ID: Lithospermum22_contig00012565
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00012565
(1814 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 341 e-136
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 341 e-136
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 343 e-136
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 342 e-136
ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222... 328 e-133
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 341 bits (875), Expect(2) = e-136
Identities = 166/296 (56%), Positives = 217/296 (73%)
Frame = +3
Query: 705 EEIENNSTKILLRRLIDVASVPNLADLYIAFSELDLQGIRKKTKGLWQEAWSIWEPIVEE 884
++ ++ K L+R+++ + PNL D Y+ FS LDLQG+ KKTK L S+WE ++ E
Sbjct: 643 DDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRE 702
Query: 885 RRNNIHGNKDFKYQDFVDTLVGQKFTNDGINQILMELFFAGSDTTTSTIEWMMSELIKNP 1064
RR + D QDF++ L+ +++D INQ+ MEL AG+DT++ST+EW M+ELIK+P
Sbjct: 703 RREG--ASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSP 760
Query: 1065 RVMNNLRKELEKEIGQDTSIKESQLLHLPYLQACLKETLRLHPSAPLLLPRRAIEMCQVM 1244
M + +EL +EI D K S L HLPYLQAC+KETLRLHPSAPLLLPRRA C+VM
Sbjct: 761 ESMKKVHEELAREIS-DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVM 819
Query: 1245 NFTIPKNAQVLVNVWAIGRDPIVWEEPLEFKPERFLNSPIDFKGNDFEFLPFGSGRRMCP 1424
N+TIPK++Q+ VN WAIGRDP+ WE+PL FKPERFLNS +DFKGN+ EF+PFG+GRR+CP
Sbjct: 820 NYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICP 879
Query: 1425 GYTMAARQIPLVIALLIQNFDWSLPKEEQPNMLDMKEKFGITLQKQQPLLLIPHTR 1592
G MAAR +PL++A L FDWSLP P+ LDM +KFGI LQK+QPLL+IP R
Sbjct: 880 GLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 935
Score = 172 bits (436), Expect(2) = e-136
Identities = 86/180 (47%), Positives = 123/180 (68%), Gaps = 1/180 (0%)
Frame = +1
Query: 91 IFKNSNKLFSTSSKLPPGPTPWPLIGNIHQLGNMNHVSLANFAKIYGPIMSFKLGHKPLI 270
+ K+S+ L S LPPGP+PWP++GN+ LGNM H+SLA F++ YGP++S +LG + L+
Sbjct: 465 LLKDSSSL--KSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILV 522
Query: 271 VASSPSIAMEILKKQDLVLSGRDAPRAFPYSRKELNQISM-WTEEYNETSKYLRSLCKVE 447
VAS+ S AMEILK D VLSGR P A P E+N++S+ W E N+ K LR++C+ E
Sbjct: 523 VASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAE 582
Query: 448 LFSGKALTSQECLRATKVLEMVEFLKAREGEVVNISQLAFATNFNLLGNILLSKDLINLE 627
LFS K + SQ + KV+EMV F+ +EGEV+ + ++ FAT N L +L+S+D I+ E
Sbjct: 583 LFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFE 642
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 341 bits (875), Expect(2) = e-136
Identities = 166/296 (56%), Positives = 217/296 (73%)
Frame = +3
Query: 705 EEIENNSTKILLRRLIDVASVPNLADLYIAFSELDLQGIRKKTKGLWQEAWSIWEPIVEE 884
++ ++ K L+R+++ + PNL D Y+ FS LDLQG+ KKTK L S+WE ++ E
Sbjct: 210 DDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRE 269
Query: 885 RRNNIHGNKDFKYQDFVDTLVGQKFTNDGINQILMELFFAGSDTTTSTIEWMMSELIKNP 1064
RR + D QDF++ L+ +++D INQ+ MEL AG+DT++ST+EW M+ELIK+P
Sbjct: 270 RREG--ASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSP 327
Query: 1065 RVMNNLRKELEKEIGQDTSIKESQLLHLPYLQACLKETLRLHPSAPLLLPRRAIEMCQVM 1244
M + +EL +EI D K S L HLPYLQAC+KETLRLHPSAPLLLPRRA C+VM
Sbjct: 328 ESMKKVHEELAREIS-DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVM 386
Query: 1245 NFTIPKNAQVLVNVWAIGRDPIVWEEPLEFKPERFLNSPIDFKGNDFEFLPFGSGRRMCP 1424
N+TIPK++Q+ VN WAIGRDP+ WE+PL FKPERFLNS +DFKGN+ EF+PFG+GRR+CP
Sbjct: 387 NYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICP 446
Query: 1425 GYTMAARQIPLVIALLIQNFDWSLPKEEQPNMLDMKEKFGITLQKQQPLLLIPHTR 1592
G MAAR +PL++A L FDWSLP P+ LDM +KFGI LQK+QPLL+IP R
Sbjct: 447 GLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 502
Score = 172 bits (436), Expect(2) = e-136
Identities = 86/180 (47%), Positives = 123/180 (68%), Gaps = 1/180 (0%)
Frame = +1
Query: 91 IFKNSNKLFSTSSKLPPGPTPWPLIGNIHQLGNMNHVSLANFAKIYGPIMSFKLGHKPLI 270
+ K+S+ L S LPPGP+PWP++GN+ LGNM H+SLA F++ YGP++S +LG + L+
Sbjct: 32 LLKDSSSL--KSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILV 89
Query: 271 VASSPSIAMEILKKQDLVLSGRDAPRAFPYSRKELNQISM-WTEEYNETSKYLRSLCKVE 447
VAS+ S AMEILK D VLSGR P A P E+N++S+ W E N+ K LR++C+ E
Sbjct: 90 VASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAE 149
Query: 448 LFSGKALTSQECLRATKVLEMVEFLKAREGEVVNISQLAFATNFNLLGNILLSKDLINLE 627
LFS K + SQ + KV+EMV F+ +EGEV+ + ++ FAT N L +L+S+D I+ E
Sbjct: 150 LFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFE 209
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 343 bits (879), Expect(2) = e-136
Identities = 166/296 (56%), Positives = 218/296 (73%)
Frame = +3
Query: 705 EEIENNSTKILLRRLIDVASVPNLADLYIAFSELDLQGIRKKTKGLWQEAWSIWEPIVEE 884
++ ++ K L+R+++ + PNL D Y+ FS LDLQG+ KKTK L S+WE ++ E
Sbjct: 210 DDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRE 269
Query: 885 RRNNIHGNKDFKYQDFVDTLVGQKFTNDGINQILMELFFAGSDTTTSTIEWMMSELIKNP 1064
RR + D QDF++ L+ +++D INQ+ MEL AG+DT++ST+EW M+ELIK+P
Sbjct: 270 RREG--ASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSP 327
Query: 1065 RVMNNLRKELEKEIGQDTSIKESQLLHLPYLQACLKETLRLHPSAPLLLPRRAIEMCQVM 1244
M + +EL +EI D K S L HLPYLQAC+KETLRLHPSAPLLLPRRA C+VM
Sbjct: 328 ESMKKVHEELAREIS-DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVM 386
Query: 1245 NFTIPKNAQVLVNVWAIGRDPIVWEEPLEFKPERFLNSPIDFKGNDFEFLPFGSGRRMCP 1424
N+TIPK++Q+ VN WAIGRDP+ WE+PL FKPERFLNS +DFKGN+ EF+PFG+GRR+CP
Sbjct: 387 NYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICP 446
Query: 1425 GYTMAARQIPLVIALLIQNFDWSLPKEEQPNMLDMKEKFGITLQKQQPLLLIPHTR 1592
G MAAR +PL++A L FDWSLP P+ LDM +KFG+TLQK+QPLL+IP R
Sbjct: 447 GLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVR 502
Score = 170 bits (430), Expect(2) = e-136
Identities = 86/180 (47%), Positives = 121/180 (67%), Gaps = 1/180 (0%)
Frame = +1
Query: 91 IFKNSNKLFSTSSKLPPGPTPWPLIGNIHQLGNMNHVSLANFAKIYGPIMSFKLGHKPLI 270
+ K+S+ L S LPPGP+PWP++GN+ LGNM H+SLA F++ YGP++S +LG + L+
Sbjct: 32 LLKDSSSL--KSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILV 89
Query: 271 VASSPSIAMEILKKQDLVLSGRDAPRAFPYSRKELNQISM-WTEEYNETSKYLRSLCKVE 447
VAS+ S AMEILK D VLSGR P A P E+N +S+ W E N K LR++C+ E
Sbjct: 90 VASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAE 149
Query: 448 LFSGKALTSQECLRATKVLEMVEFLKAREGEVVNISQLAFATNFNLLGNILLSKDLINLE 627
LFS K + SQ + KV+EMV F+ +EGEV+ + ++ FAT N L +L+S+D I+ E
Sbjct: 150 LFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFE 209
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 342 bits (878), Expect(2) = e-136
Identities = 166/296 (56%), Positives = 218/296 (73%)
Frame = +3
Query: 705 EEIENNSTKILLRRLIDVASVPNLADLYIAFSELDLQGIRKKTKGLWQEAWSIWEPIVEE 884
++ ++ K L+R+++ + PNL D Y+ FS LDLQG+ KKTK L S+WE ++ E
Sbjct: 774 DDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRE 833
Query: 885 RRNNIHGNKDFKYQDFVDTLVGQKFTNDGINQILMELFFAGSDTTTSTIEWMMSELIKNP 1064
RR + D QDF++ L+ +++D INQ+ MEL AG+DT++ST+EW M+ELIK+P
Sbjct: 834 RREG--ASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSP 891
Query: 1065 RVMNNLRKELEKEIGQDTSIKESQLLHLPYLQACLKETLRLHPSAPLLLPRRAIEMCQVM 1244
M + +EL +EI D K S L HLPYLQAC+KETLRLHPSAPLLLPRRA C+VM
Sbjct: 892 ESMKKVHEELAREIS-DNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVM 950
Query: 1245 NFTIPKNAQVLVNVWAIGRDPIVWEEPLEFKPERFLNSPIDFKGNDFEFLPFGSGRRMCP 1424
N+TIPK++Q+ VN WAIGRDP+ WE+PL FKPERFLNS +DFKGN+ EF+PFG+GRR+CP
Sbjct: 951 NYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICP 1010
Query: 1425 GYTMAARQIPLVIALLIQNFDWSLPKEEQPNMLDMKEKFGITLQKQQPLLLIPHTR 1592
G MAAR +PL++A L FDWSLP P+ LDM +KFG+TLQK+QPLL+IP R
Sbjct: 1011 GLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKKR 1066
Score = 170 bits (430), Expect(2) = e-136
Identities = 86/180 (47%), Positives = 121/180 (67%), Gaps = 1/180 (0%)
Frame = +1
Query: 91 IFKNSNKLFSTSSKLPPGPTPWPLIGNIHQLGNMNHVSLANFAKIYGPIMSFKLGHKPLI 270
+ K+S+ L S LPPGP+PWP++GN+ LGNM H+SLA F++ YGP++S +LG + L+
Sbjct: 596 LLKDSSSL--KSPPLPPGPSPWPILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILV 653
Query: 271 VASSPSIAMEILKKQDLVLSGRDAPRAFPYSRKELNQISM-WTEEYNETSKYLRSLCKVE 447
VAS+ S AMEILK D VLSGR P A P E+N +S+ W E N K LR++C+ E
Sbjct: 654 VASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAE 713
Query: 448 LFSGKALTSQECLRATKVLEMVEFLKAREGEVVNISQLAFATNFNLLGNILLSKDLINLE 627
LFS K + SQ + KV+EMV F+ +EGEV+ + ++ FAT N L +L+S+D I+ E
Sbjct: 714 LFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFE 773
>ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222845316|gb|EEE82863.1|
cytochrome P450 [Populus trichocarpa]
Length = 507
Score = 328 bits (842), Expect(2) = e-133
Identities = 163/301 (54%), Positives = 216/301 (71%), Gaps = 6/301 (1%)
Frame = +3
Query: 708 EIENNSTKILLRRLIDVASVPNLADLYIAFSELDLQGIRKKTKGLWQEAWSIWEPIVEER 887
E E+ +L+ + +AS PN++D Y LDLQG+RKKT L + ++++ E I++ER
Sbjct: 206 ESEDGGMSSVLKDIARLASTPNISDFYPILGPLDLQGLRKKTMELHRRSFNMCEAIIQER 265
Query: 888 RNNIHGNKD----FKYQDFVDTLVGQKFTNDGINQILMELFFAGSDTTTSTIEWMMSELI 1055
R G +D + +DF+D L+ ++D I+ +LMEL AG+DT++STIEW M+ELI
Sbjct: 266 REGGEGKRDGPDASRRRDFLDALILNGSSDDQIDILLMELLSAGTDTSSSTIEWTMAELI 325
Query: 1056 KNPRVMNNLRKELEKEIG--QDTSIKESQLLHLPYLQACLKETLRLHPSAPLLLPRRAIE 1229
KNPR + +++E+ I +DT KES L L YLQAC+KETLRLHP P LLP RAI+
Sbjct: 326 KNPRCLKKVQEEIANVINMNRDTGFKESHLPQLTYLQACVKETLRLHPPGPFLLPHRAID 385
Query: 1230 MCQVMNFTIPKNAQVLVNVWAIGRDPIVWEEPLEFKPERFLNSPIDFKGNDFEFLPFGSG 1409
CQVMN+TIPKN QVLVN WAIGRDP WEEP+ F PERFL+S +DFKGNDFEF+PFGSG
Sbjct: 386 SCQVMNYTIPKNTQVLVNYWAIGRDPKSWEEPVVFNPERFLSSNLDFKGNDFEFIPFGSG 445
Query: 1410 RRMCPGYTMAARQIPLVIALLIQNFDWSLPKEEQPNMLDMKEKFGITLQKQQPLLLIPHT 1589
RR+CPG MAA+ + L+IA LI FDWSLP + P LDM E +G+TL+K+QPLLL+P +
Sbjct: 446 RRICPGLPMAAKHVALIIAYLILFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVPTS 505
Query: 1590 R 1592
+
Sbjct: 506 K 506
Score = 175 bits (444), Expect(2) = e-133
Identities = 87/171 (50%), Positives = 120/171 (70%), Gaps = 1/171 (0%)
Frame = +1
Query: 118 STSSKLPPGPTPWPLIGNIHQLGNMNHVSLANFAKIYGPIMSFKLGHKPLIVASSPSIAM 297
++S PPGP PWP++GNI QLG+ H++L +FAKI+GPI S +LG + ++V SS + A+
Sbjct: 34 NSSPPFPPGPYPWPILGNILQLGDKPHITLTHFAKIHGPIFSLRLGTQLVVVGSSQAAAI 93
Query: 298 EILKKQDLVLSGRDAPRAFPYSRKELNQISM-WTEEYNETSKYLRSLCKVELFSGKALTS 474
ILK D +LSGR P P ELN++S+ W E NE +YLR++CK ELFS KAL S
Sbjct: 94 AILKTHDRILSGRHVPHMAPSKSSELNKLSLGWVVECNERWRYLRTICKSELFSLKALES 153
Query: 475 QECLRATKVLEMVEFLKAREGEVVNISQLAFATNFNLLGNILLSKDLINLE 627
Q C+R K EM+ F+ EG+VV I ++A AT FN+L NIL+S+DL++LE
Sbjct: 154 QACIRERKAKEMIGFINKMEGKVVKIREVATATVFNMLSNILVSRDLVSLE 204