BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00006571
         (2064 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]   871   0.0  
ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis v...   870   0.0  
sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=A...   867   0.0  
ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trich...   824   0.0  
ref|XP_002299782.1| predicted protein [Populus trichocarpa] gi|2...   816   0.0  

>emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]
          Length = 558

 Score =  871 bits (2250), Expect = 0.0
 Identities = 412/548 (75%), Positives = 455/548 (83%)
 Frame = +3

Query: 153  AKTRHFKWEVEYMHWAPDCKEGVVMGINGQFPGPTIQARAGDTIHLELTNHLHTEGVVIH 332
            +KTRHFKWEVEYM+W+PDC EGVVMGINGQFPGPTI+A AGDTI +ELTN LHTEGVVIH
Sbjct: 6    SKTRHFKWEVEYMYWSPDCMEGVVMGINGQFPGPTIRAVAGDTIVVELTNRLHTEGVVIH 65

Query: 333  WHGIRQIDTPWADGTASISQCPINPGESFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLI 512
            WHGIRQ  TPWADGTASISQC INPGE+F+YR+KVDKAGTYFYHGHYGMQRSAGLYGSL 
Sbjct: 66   WHGIRQFGTPWADGTASISQCAINPGETFIYRYKVDKAGTYFYHGHYGMQRSAGLYGSLX 125

Query: 513  VDVEEGKKEPFHYDGEFNLLLSDLWHKSAHEQEVDLSSKPMRWIGEPQSLLINGRGQNNC 692
            V+V EG+KEPFHYDGEFNLLLSD WHK + EQEV LSSKP RWIGEPQ+LLINGRGQ NC
Sbjct: 126  VEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWIGEPQTLLINGRGQYNC 185

Query: 693  SLAAGFNNSTLSKCKLRGNEQCAPQILQVLPNKTYRLRVXXXXXXXXXXXXIGNHKMIIV 872
            SLAA   NS+  +C+ RGNEQCAPQIL V P+KTYRLRV            IGNHKM++V
Sbjct: 186  SLAAHVTNSSSPQCQFRGNEQCAPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMV 245

Query: 873  EADGNYIQPFEVDNMDIYSGDSYSVLFTTNQDPSINYWISVSVRGREPKTPQGLTILNYK 1052
            EADGNYIQP  VD++DIYSG+SYSVL  T+QDPS NYWISVSVRGREPKTPQGLTILNY+
Sbjct: 246  EADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSXNYWISVSVRGREPKTPQGLTILNYQ 305

Query: 1053 TTSASKLXXXXXXXXXXWNDYNHSKSFTFKILSQMGSPKPPVRPHRRIVLLNTQNFIDGQ 1232
            TTSASKL          WNDYNHSKSF+ KIL+ MGSPKPP   +RRI+LLNTQN I+G 
Sbjct: 306  TTSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGF 365

Query: 1233 TKWSINNVSLVLPATPLLGSMKYNLKGAFDPKKPPESFSANYDVMXXXXXXXXXXGSLVY 1412
            TKW+INN+SL LP TP LG++K+ L  AFD K PPE+F  +YDVM          G+ VY
Sbjct: 366  TKWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVY 425

Query: 1413 TLRFNTTIDVILQNANALAANVSEIHPWHLHGHDFWVLGYGEGRFKEGDEKKFNLKNPPL 1592
             L F TT+DVILQNANALA  VSEIHPWHLHGHDFWVLGYGEG+F+E D K+FNLKNPPL
Sbjct: 426  MLEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPL 485

Query: 1593 RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVRRIGKIPDEALTCG 1772
            RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGV  +  +P+ AL CG
Sbjct: 486  RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACG 545

Query: 1773 LTAKMFMS 1796
            LT KM MS
Sbjct: 546  LTGKMLMS 553


>ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis vinifera]
          Length = 581

 Score =  870 bits (2249), Expect = 0.0
 Identities = 411/548 (75%), Positives = 456/548 (83%)
 Frame = +3

Query: 153  AKTRHFKWEVEYMHWAPDCKEGVVMGINGQFPGPTIQARAGDTIHLELTNHLHTEGVVIH 332
            +KTRHFKWEVEYM+W+PDC EGVVMGINGQFPGPTI+A AGDTI +ELTN LHTEGVVIH
Sbjct: 29   SKTRHFKWEVEYMYWSPDCMEGVVMGINGQFPGPTIRAVAGDTIVVELTNRLHTEGVVIH 88

Query: 333  WHGIRQIDTPWADGTASISQCPINPGESFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLI 512
            WHGIRQ  TPWADGTASISQC INPGE+F+YR+KVDKAGTYFYHGHYGMQRSAGLYGSL+
Sbjct: 89   WHGIRQFGTPWADGTASISQCAINPGETFIYRYKVDKAGTYFYHGHYGMQRSAGLYGSLV 148

Query: 513  VDVEEGKKEPFHYDGEFNLLLSDLWHKSAHEQEVDLSSKPMRWIGEPQSLLINGRGQNNC 692
            V+V EG+KEPFHYDGEFNLLLSD WHK + EQEV LSSKP RWIGEPQ+LLINGRGQ NC
Sbjct: 149  VEVGEGQKEPFHYDGEFNLLLSDWWHKGSQEQEVALSSKPFRWIGEPQTLLINGRGQYNC 208

Query: 693  SLAAGFNNSTLSKCKLRGNEQCAPQILQVLPNKTYRLRVXXXXXXXXXXXXIGNHKMIIV 872
            SLAA   NS+  +C+ RGNEQC+PQIL V P+KTYRLRV            IGNHKM++V
Sbjct: 209  SLAAHVTNSSSPQCQFRGNEQCSPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMV 268

Query: 873  EADGNYIQPFEVDNMDIYSGDSYSVLFTTNQDPSINYWISVSVRGREPKTPQGLTILNYK 1052
            EADGNYIQP  VD++DIYSG+SYSVL  T+QDPS NYWISVSVRGREPKTPQGLTILNY+
Sbjct: 269  EADGNYIQPVAVDDLDIYSGESYSVLIHTDQDPSKNYWISVSVRGREPKTPQGLTILNYQ 328

Query: 1053 TTSASKLXXXXXXXXXXWNDYNHSKSFTFKILSQMGSPKPPVRPHRRIVLLNTQNFIDGQ 1232
            TTSASKL          WNDYNHSKSF+ KIL+ MGSPKPP   +RRI+LLNTQN I+G 
Sbjct: 329  TTSASKLPTSTPPVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGF 388

Query: 1233 TKWSINNVSLVLPATPLLGSMKYNLKGAFDPKKPPESFSANYDVMXXXXXXXXXXGSLVY 1412
            TKW+INN+SL LP TP LG++K+ L  AFD K PPE+F  +YDVM          G+ VY
Sbjct: 389  TKWAINNISLTLPPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVY 448

Query: 1413 TLRFNTTIDVILQNANALAANVSEIHPWHLHGHDFWVLGYGEGRFKEGDEKKFNLKNPPL 1592
             L F TT+DVILQNANALA  VSEIHPWHLHGHDFWVLGYGEG+F+E D K+FNLKNPPL
Sbjct: 449  MLEFRTTVDVILQNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPL 508

Query: 1593 RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVRRIGKIPDEALTCG 1772
            RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGV  +  +P+ AL CG
Sbjct: 509  RNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACG 568

Query: 1773 LTAKMFMS 1796
            LT KM MS
Sbjct: 569  LTGKMLMS 576


>sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags:
            Precursor gi|599594|dbj|BAA07734.1| ascorbate oxidase
            precursor [Nicotiana tabacum]
          Length = 578

 Score =  867 bits (2239), Expect = 0.0
 Identities = 412/572 (72%), Positives = 466/572 (81%), Gaps = 3/572 (0%)
 Frame = +3

Query: 99   LSFFLIVFIV---NNSFGAFGAKTRHFKWEVEYMHWAPDCKEGVVMGINGQFPGPTIQAR 269
            L FFL+  I+   ++S     AKTRHFKW+VEY+HW+PD +E VVMGINGQFPGPTI+A+
Sbjct: 7    LFFFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAK 66

Query: 270  AGDTIHLELTNHLHTEGVVIHWHGIRQIDTPWADGTASISQCPINPGESFVYRFKVDKAG 449
            AGDT+ + LTN LHTEGVVIHWHGIRQI TPWADGTA+ISQC INPGE+F+YRFKVDKAG
Sbjct: 67   AGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAG 126

Query: 450  TYFYHGHYGMQRSAGLYGSLIVDVEEGKKEPFHYDGEFNLLLSDLWHKSAHEQEVDLSSK 629
            TYFYHGHYGMQRSAGLYGSLIV+V EG+KEPFHYDGEFNLLLSD WHK +HEQEVDLSS 
Sbjct: 127  TYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSSN 186

Query: 630  PMRWIGEPQSLLINGRGQNNCSLAAGFNNSTLSKCKLRGNEQCAPQILQVLPNKTYRLRV 809
            P+RWIGEPQ+LL+NGRGQ NCSLAA F+   L +CKLRG EQ APQIL+V PNK YRLRV
Sbjct: 187  PLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLRV 246

Query: 810  XXXXXXXXXXXXIGNHKMIIVEADGNYIQPFEVDNMDIYSGDSYSVLFTTNQDPSINYWI 989
                        IG HKM++VEADGNY+QPF V +MDIYSG+SYSVLF T+QDP+ NYWI
Sbjct: 247  ASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYWI 306

Query: 990  SVSVRGREPKTPQGLTILNYKTTSASKLXXXXXXXXXXWNDYNHSKSFTFKILSQMGSPK 1169
            S++VRGREPKTPQGLT+LNY   SASK           WNDYNHSKSF+ KI + MGSPK
Sbjct: 307  SINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSPK 366

Query: 1170 PPVRPHRRIVLLNTQNFIDGQTKWSINNVSLVLPATPLLGSMKYNLKGAFDPKKPPESFS 1349
            PP + HRRI+LLNTQN IDG TKW+INNVSLVLP    LGS++Y +  AFD K PP++F 
Sbjct: 367  PPPQNHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGI-NAFDTKPPPDNFP 425

Query: 1350 ANYDVMXXXXXXXXXXGSLVYTLRFNTTIDVILQNANALAANVSEIHPWHLHGHDFWVLG 1529
             +YDV+          G+ VY L+FNTTID+ILQNANALA +VSEIHPWHLHGHDFWVLG
Sbjct: 426  KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGHDFWVLG 485

Query: 1530 YGEGRFKEGDEKKFNLKNPPLRNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMG 1709
            YGEG+F E D KKFNLKNPPLRNTAVIFPFGWTALRFV DNPGVWAFHCHIEPHLHMGMG
Sbjct: 486  YGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPHLHMGMG 545

Query: 1710 VVFAEGVRRIGKIPDEALTCGLTAKMFMSTKN 1805
            V+FAEGV  + KIP EAL CGLT KM MS K+
Sbjct: 546  VIFAEGVHLVKKIPKEALACGLTGKMLMSNKH 577


>ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trichocarpa]
            gi|222849246|gb|EEE86793.1| l-ascorbate oxidase precursor
            [Populus trichocarpa]
          Length = 597

 Score =  824 bits (2129), Expect = 0.0
 Identities = 397/556 (71%), Positives = 449/556 (80%), Gaps = 6/556 (1%)
 Frame = +3

Query: 156  KTR--HF-KWEVEYMHWAPDCKEGVVMGINGQFPGPTIQARAGDTIHLELTNHLHTEGVV 326
            KTR  HF KWEVEYM+W+PD  E VVMGING+FPGPTI+ARAGDT+H+ LTN LHTEGVV
Sbjct: 40   KTRSLHFYKWEVEYMYWSPDGLENVVMGINGKFPGPTIRARAGDTVHVHLTNKLHTEGVV 99

Query: 327  IHWHGIRQIDTPWADGTASISQCPINPGESFVYRFKVDKAGTYFYHGHYGMQRSAGLYGS 506
            IHWHGIRQ  TPWADGTASISQC INPGESF YRF VD+AGTYFYHGHYGMQRSAGLYGS
Sbjct: 100  IHWHGIRQKGTPWADGTASISQCAINPGESFDYRFTVDRAGTYFYHGHYGMQRSAGLYGS 159

Query: 507  LIVDVEEGKKEPFHYDGEFNLLLSDLWHKSAHEQEVDLSSKPMRWIGEPQSLLINGRGQN 686
            LIVDV EG+KEPFHYDGEF+LLLSD WH+SAH QEV LSS+PMRWIGEPQ+LL+NGRGQ 
Sbjct: 160  LIVDVAEGEKEPFHYDGEFDLLLSDWWHESAHHQEVGLSSRPMRWIGEPQTLLVNGRGQY 219

Query: 687  NCSLAAGF-NNSTLSKCKLRGNEQCAPQILQVLPNKTYRLRVXXXXXXXXXXXXIGNHKM 863
             CSLAA + NNS+LS+C + G+EQ AP IL V PNKTYR+R+            IGNHKM
Sbjct: 220  GCSLAAHYSNNSSLSQCNVTGHEQWAPYILHVDPNKTYRIRLSSTTALASLNLAIGNHKM 279

Query: 864  IIVEADGNYIQPFEVDNMDIYSGDSYSVLFTTNQDPSINYWISVSVRGREPKTPQGLTIL 1043
            ++VEADGNY+QPFE D++DIYSG+SYSVL  T+QDPS NYWIS  VRGR+P+TPQ LTIL
Sbjct: 280  LVVEADGNYLQPFETDDLDIYSGESYSVLLKTSQDPSQNYWISFGVRGRKPQTPQALTIL 339

Query: 1044 NYKTTSASKLXXXXXXXXXXWNDYNHSKSFTFKI--LSQMGSPKPPVRPHRRIVLLNTQN 1217
            NYKT SASK           W+DY HSK+FT K+  L     PKPP   HRRI+LLNTQN
Sbjct: 340  NYKTNSASKFPLSPPPVTPRWDDYAHSKAFTNKVKALDHKTIPKPPSTYHRRIILLNTQN 399

Query: 1218 FIDGQTKWSINNVSLVLPATPLLGSMKYNLKGAFDPKKPPESFSANYDVMXXXXXXXXXX 1397
             ++G TKWSINNVSL LPATP LGS+++ L+  FD  KPPESF   YDVM          
Sbjct: 400  KMNGYTKWSINNVSLSLPATPYLGSIRFGLQNGFDQTKPPESFPVQYDVMKPPGNPNTTT 459

Query: 1398 GSLVYTLRFNTTIDVILQNANALAANVSEIHPWHLHGHDFWVLGYGEGRFKEGDEKKFNL 1577
            G+ VY L + +T+DVILQNANALA NVSEIHPWHLHGHDFWVLGYGEG+F + DEKKFN+
Sbjct: 460  GNGVYMLSYYSTVDVILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFTKDDEKKFNM 519

Query: 1578 KNPPLRNTAVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVRRIGKIPDE 1757
            KNPP RN+AVIFP+GWTALRFVADNPGVWAFHCHIEPHLHMGMGVV AEGV+R+ KIP E
Sbjct: 520  KNPPYRNSAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVLAEGVQRLPKIPKE 579

Query: 1758 ALTCGLTAKMFMSTKN 1805
            AL+CGLT K FM+  N
Sbjct: 580  ALSCGLTGKKFMTGNN 595


>ref|XP_002299782.1| predicted protein [Populus trichocarpa] gi|222847040|gb|EEE84587.1|
            predicted protein [Populus trichocarpa]
          Length = 566

 Score =  816 bits (2109), Expect = 0.0
 Identities = 388/545 (71%), Positives = 438/545 (80%), Gaps = 2/545 (0%)
 Frame = +3

Query: 168  FKWEVEYMHWAPDCKEGVVMGINGQFPGPTIQARAGDTIHLELTNHLHTEGVVIHWHGIR 347
            +KWEVEYM+W+PD  E VVMGINGQFPGPTI+ARAGD +H++LTN LHTEGVVIHWHGIR
Sbjct: 17   YKWEVEYMYWSPDGIENVVMGINGQFPGPTIRARAGDIVHVQLTNKLHTEGVVIHWHGIR 76

Query: 348  QIDTPWADGTASISQCPINPGESFVYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVDVEE 527
            Q  TPWADGTASISQC INPGE+F YRF VD+AGTYFYHGHYGMQRSAGLYGS+IVDV E
Sbjct: 77   QKGTPWADGTASISQCVINPGETFDYRFTVDRAGTYFYHGHYGMQRSAGLYGSMIVDVAE 136

Query: 528  GKKEPFHYDGEFNLLLSDLWHKSAHEQEVDLSSKPMRWIGEPQSLLINGRGQNNCSLAAG 707
            G+KEPFHYDGEF+LLLSD WHKS H QEV LSS+PMRWI EPQ+LLINGRGQ  CSLAA 
Sbjct: 137  GEKEPFHYDGEFDLLLSDWWHKSVHHQEVGLSSRPMRWINEPQALLINGRGQYGCSLAAH 196

Query: 708  FNNSTLSKCKLRGNEQCAPQILQVLPNKTYRLRVXXXXXXXXXXXXIGNHKMIIVEADGN 887
              NS+ S+C + G+EQ AP IL V PNKTYR+R+            IGNHKM++VEADGN
Sbjct: 197  HGNSSFSQCNITGHEQWAPYILHVDPNKTYRIRIASTTALASLNLAIGNHKMMVVEADGN 256

Query: 888  YIQPFEVDNMDIYSGDSYSVLFTTNQDPSINYWISVSVRGREPKTPQGLTILNYKTTSAS 1067
            Y+QPFE +++DIYSG+SYSVL  T+QD S NYWIS  VRGREP TPQ LTILNYKT SAS
Sbjct: 257  YLQPFETNDLDIYSGESYSVLLKTSQDSSQNYWISFGVRGREPHTPQALTILNYKTNSAS 316

Query: 1068 KLXXXXXXXXXXWNDYNHSKSFTFKI--LSQMGSPKPPVRPHRRIVLLNTQNFIDGQTKW 1241
            KL          W+DY HSK+F+ KI  L     PKP    HRRIVLLNTQN ++G TKW
Sbjct: 317  KLPLSPPPVTPRWDDYAHSKAFSNKIKALDHKSIPKPSPTYHRRIVLLNTQNKMNGYTKW 376

Query: 1242 SINNVSLVLPATPLLGSMKYNLKGAFDPKKPPESFSANYDVMXXXXXXXXXXGSLVYTLR 1421
            SINNVSL +PATP LGS+++ L+GAFD  KPPE F  +YDVM          GS VY L 
Sbjct: 377  SINNVSLSIPATPYLGSIRFGLRGAFDQTKPPEDFPGHYDVMKPPVNPNTTTGSGVYMLG 436

Query: 1422 FNTTIDVILQNANALAANVSEIHPWHLHGHDFWVLGYGEGRFKEGDEKKFNLKNPPLRNT 1601
            + +T+DVILQNANAL  NVSEIHPWHLHGHDFWVLGYGEG+F + DEKKFNLKNPP RNT
Sbjct: 437  YYSTVDVILQNANALTENVSEIHPWHLHGHDFWVLGYGEGKFTKSDEKKFNLKNPPYRNT 496

Query: 1602 AVIFPFGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVRRIGKIPDEALTCGLTA 1781
            AVIFP+GWTALRFVADNPGVWAFHCHIEPHLH+GMGVVFAEGV+ + KIP EAL+CGLT 
Sbjct: 497  AVIFPYGWTALRFVADNPGVWAFHCHIEPHLHLGMGVVFAEGVQHVAKIPKEALSCGLTG 556

Query: 1782 KMFMS 1796
            K FM+
Sbjct: 557  KRFMT 561